Lag0003228.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0003228.1 vs. NCBI nr
Match: XP_022148051.1 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 [Momordica charantia])

HSP 1 Score: 620.5 bits (1599), Expect = 1.0e-173
Identity = 318/474 (67.09%), Postives = 358/474 (75.53%), Query Frame = 0

Query: 1   MATCDGSRTKLVLLIAFLFFNNIVAEREWVSDATAEQSGKRWALLVAGSNGYDNYRHQAD 60
           MATC  + TK VLLIA L F++I+A+RE V  A+ +Q GKRWA+LVAGS+GY+NYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 61  VCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKHV 120
           VCHAYQILRKGGL D+NIIVFMYDDIAF+PSNPRPGV+INKPDGVDVYQGVPKDYTG+HV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 121 NAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLID 180
           N+INFYAVILGN+SALTGGSGKV++S  HDHIFIYYTDHG+AG+LGMPEGDY+Y+KDL++
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 181 VLKKNMKPKVTK-------AC--------------------------------------- 240
           VLK+  + K  K       AC                                       
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 241 ---------------------DKHDLSKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNKS 300
                                DKHDLSKETL QQY  VRRRTLVDKFGYGSHVMLYGNKS
Sbjct: 241 PPTDYDTCLGDLYSIAWMEDSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKS 300

Query: 301 IGNSFLNIYIGNSPDN-NYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASLIHYWYKFQ 360
           IGN+ L+ YIG +PDN NY S VQS   I+ PSKLL     + AVSQ DASLIHYW+KFQ
Sbjct: 301 IGNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLL----YSNAVSQRDASLIHYWHKFQ 360

Query: 361 NAPFGSREKMQAREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRRQGQSLIDD 407
            APFGSREK +AR+QLEDEILNRRH D SI HI +LLFG A SSEVLNNVR+QGQSL+DD
Sbjct: 361 KAPFGSREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDD 420

BLAST of Lag0003228.1 vs. NCBI nr
Match: XP_038880044.1 (vacuolar-processing enzyme-like [Benincasa hispida])

HSP 1 Score: 619.8 bits (1597), Expect = 1.7e-173
Identity = 320/476 (67.23%), Postives = 354/476 (74.37%), Query Frame = 0

Query: 1   MATCDGSRTK-LVLLIAFLFFNNIVAEREWVSDATAEQSGKRWALLVAGSNGYDNYRHQA 60
           M TC+ S TK LV+LI F+FFNNI+A++EW+SDATA Q+GKRWALLVAGSNGY+NYRHQA
Sbjct: 1   MTTCNRSPTKLLVILIIFVFFNNIIAKKEWISDATANQTGKRWALLVAGSNGYENYRHQA 60

Query: 61  DVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKH 120
           DVCHAYQIL+KGG+ D+NIIVFMYDDIAFHP+NPR G+IINKP G DVYQGVPKDYTGK 
Sbjct: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPNNPRQGIIINKPGGPDVYQGVPKDYTGKQ 120

Query: 121 VNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLI 180
           VNA+NFYA + GNKSALTGGSGKVI+SG HDHIFIYY+DHGAAGMLGMPE DY++  DLI
Sbjct: 121 VNAVNFYAALSGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPEDDYVFVNDLI 180

Query: 181 DVLKKNMKPKVTK-------AC-------------------------------------- 240
           D LK+  K K  K       AC                                      
Sbjct: 181 DALKRKHKAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATEDSWATYCPKESR 240

Query: 241 -----------------------DKHDLSKETLNQQYHVVRRRTLVDKFGYGSHVMLYGN 300
                                  DKHDLSKETLNQQYH VRRRTLVDK G GSHVMLYGN
Sbjct: 241 GPPPKDYNTCLGDLYSVAWMEDSDKHDLSKETLNQQYHAVRRRTLVDKSGVGSHVMLYGN 300

Query: 301 KSIGNSFLNIYIGNSPD-NNYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASLIHYWYK 360
           + IG  FLN +IGNSPD NNY S VQS H ISSPSK   LSSN   VSQ DASLI+Y YK
Sbjct: 301 RRIGTDFLNTFIGNSPDYNNYKSSVQSIHTISSPSK---LSSN--GVSQRDASLIYYQYK 360

Query: 361 FQNAPFGSREKMQAREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRRQGQSLI 407
           FQ AP GSREKM+AREQLEDEIL RRHAD SIN+IG+LLFG A SS+VLN VR QGQSL+
Sbjct: 361 FQKAPIGSREKMEAREQLEDEILKRRHADNSINNIGKLLFGQARSSKVLNKVREQGQSLV 420

BLAST of Lag0003228.1 vs. NCBI nr
Match: XP_022148043.1 (vacuolar-processing enzyme-like isoform X1 [Momordica charantia])

HSP 1 Score: 607.4 bits (1565), Expect = 8.7e-170
Identity = 318/508 (62.60%), Postives = 358/508 (70.47%), Query Frame = 0

Query: 1   MATCDGSRTKLVLLIAFLFFNNIVAEREWVSDATAEQSGKRWALLVAGSNGYDNYRHQAD 60
           MATC  + TK VLLIA L F++I+A+RE V  A+ +Q GKRWA+LVAGS+GY+NYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 61  VCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKHV 120
           VCHAYQILRKGGL D+NIIVFMYDDIAF+PSNPRPGV+INKPDGVDVYQGVPKDYTG+HV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 121 NAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLID 180
           N+INFYAVILGN+SALTGGSGKV++S  HDHIFIYYTDHG+AG+LGMPEGDY+Y+KDL++
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 181 VLKKNMKPKVTK-------AC--------------------------------------- 240
           VLK+  + K  K       AC                                       
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 241 -------------------------------------------------------DKHDL 300
                                                                  DKHDL
Sbjct: 241 PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL 300

Query: 301 SKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNKSIGNSFLNIYIGNSPDN-NYASFVQSA 360
           SKETL QQY  VRRRTLVDKFGYGSHVMLYGNKSIGN+ L+ YIG +PDN NY S VQS 
Sbjct: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360

Query: 361 HNISSPSKLLSLSSNNAAVSQHDASLIHYWYKFQNAPFGSREKMQAREQLEDEILNRRHA 407
             I+ PSKLL     + AVSQ DASLIHYW+KFQ APFGSREK +AR+QLEDEILNRRH 
Sbjct: 361 DTIAPPSKLL----YSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHV 420

BLAST of Lag0003228.1 vs. NCBI nr
Match: TYK28710.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa])

HSP 1 Score: 590.1 bits (1520), Expect = 1.4e-164
Identity = 308/466 (66.09%), Postives = 341/466 (73.18%), Query Frame = 0

Query: 1   MATCDGSRTK-LVLLIAFLFFNNIVAEREWVSDATAEQSGKRWALLVAGSNGYDNYRHQA 60
           M TCD + TK L++LI FL FNNI+A+ EW+SDAT +Q+GK WALL+AGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKH 120
                      GG+ D+NIIVFMYDDIAFHPSNPRPG+IINKPDG DVY GVPKDYTGKH
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLI 180
           VNA+NFYA ILGNKSALTGGSGKVI+SG  DHIFIYY+DHGAAGMLGMPEGDYI+  DLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKKNMKPK-------VTKAC-------------------------------------- 240
           DVLK+  K K         +AC                                      
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 -------------DKHDLSKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNKSIGNSFLNI 300
                        DKHDLSKETLNQQYH VRRRT VDK GYGSHVMLYGNK IGN FL+ 
Sbjct: 241 RSPKDYNTCLGDFDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDT 300

Query: 301 YIGNSPD-NNYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASLIHYWYKFQNAPFGSRE 360
           ++GNSPD +NY   VQS   ISS SK   LSSN  +VSQ DASLIHY YKFQ APFGSRE
Sbjct: 301 FLGNSPDYDNYKYSVQSPQTISSLSK---LSSN--SVSQRDASLIHYQYKFQEAPFGSRE 360

Query: 361 KMQAREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRRQGQSLIDDWGCFRTFV 407
           KM+AREQLEDEI  RRHAD SIN IG+LLFG A SSE+L+NVR QGQSL+DDW CF+TFV
Sbjct: 361 KMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFV 420

BLAST of Lag0003228.1 vs. NCBI nr
Match: KAA0046547.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa])

HSP 1 Score: 586.6 bits (1511), Expect = 1.6e-163
Identity = 308/475 (64.84%), Postives = 341/475 (71.79%), Query Frame = 0

Query: 1   MATCDGSRTK-LVLLIAFLFFNNIVAEREWVSDATAEQSGKRWALLVAGSNGYDNYRHQA 60
           M TCD + TK L++LI FL FNNI+A+ EW+SDAT +Q+GK WALL+AGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKH 120
                      GG+ D+NIIVFMYDDIAFHPSNPRPG+IINKPDG DVY GVPKDYTGKH
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLI 180
           VNA+NFYA ILGNKSALTGGSGKVI+SG  DHIFIYY+DHGAAGMLGMPEGDYI+  DLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKKNMKPK-------VTKAC-------------------------------------- 240
           DVLK+  K K         +AC                                      
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 ----------------------DKHDLSKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNK 300
                                 DKHDLSKETLNQQYH VRRRT VDK GYGSHVMLYGNK
Sbjct: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300

Query: 301 SIGNSFLNIYIGNSPD-NNYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASLIHYWYKF 360
            IGN FL+ ++GNSPD +NY   VQS   ISS SK   LSSN  +VSQ DASLIHY YKF
Sbjct: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSK---LSSN--SVSQRDASLIHYQYKF 360

Query: 361 QNAPFGSREKMQAREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRRQGQSLID 407
           Q APFGSREKM+AREQLEDEI  RRHAD SIN IG+LLFG A SSE+L+NVR QGQSL+D
Sbjct: 361 QEAPFGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVD 420

BLAST of Lag0003228.1 vs. ExPASy Swiss-Prot
Match: P49044 (Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.2e-105
Identity = 205/431 (47.56%), Postives = 261/431 (60.56%), Query Frame = 0

Query: 39  GKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVI 98
           G RWA+L+AGSNGY NYRHQ+DVCHAYQ+LRKGG  ++NIIVFMYDDIA +  NPRPGVI
Sbjct: 54  GTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDIASNEENPRPGVI 113

Query: 99  INKPDGVDVYQGVPKDYTGKHVNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTD 158
           INKPDG DVY GVPKDYTG  V+A NFYA +LGNKSALTGGSGKV++SG +DHIF+YYTD
Sbjct: 114 INKPDGDDVYAGVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHIFVYYTD 173

Query: 159 HGAAGMLGMPEGDYIYSKDLIDVLKKNMKPKVTK-------ACDK--------------- 218
           HG  G+LGMP G Y+Y+ DL +VLKK       K       AC+                
Sbjct: 174 HGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPDDLNIY 233

Query: 219 ---------------------------------------------HDLSKETLNQQYHVV 278
                                                        H+L  E+L QQY +V
Sbjct: 234 ATTASNAEESSWGYYCPGDKPPPPPEYSTCLGDLYSIAWMEDSEVHNLQTESLQQQYKLV 293

Query: 279 RRRTLVDKFGYGSHVMLYGNKSIGNSFLNIYIGNSPDNNYASFVQSAHNISSPSKLLSLS 338
           + RT+ +   YGSHVM YG+  +  + L  Y+G +P N+  SFV    N       L L 
Sbjct: 294 KNRTISEP--YGSHVMEYGDIGLSKNDLYQYLGTNPANDNNSFVDETEN------SLKLR 353

Query: 339 SNNAAVSQHDASLIHYWYKFQNAPFGSREKMQAREQLEDEILNRRHADYSINHIGELLFG 398
           + +AAV+Q DA LIH+W KF+ AP GS +K +A +Q+ + + +R+H D S+  IG+LLFG
Sbjct: 354 TPSAAVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVLEAMSHRKHIDNSVKLIGQLLFG 413

Query: 399 HANSSEVLNNVRRQGQSLIDDWGCFRTFVKTYEKHCKRLSRYGMKYTRALANICNAGITI 403
               +E+L+ VR  G  L+D+W C +T VKT+E HC  LS+YGMK+ R+ ANICNAGI  
Sbjct: 414 IEKGTELLDVVRPAGSPLVDNWDCLKTMVKTFETHCGSLSQYGMKHMRSFANICNAGIPN 473

BLAST of Lag0003228.1 vs. ExPASy Swiss-Prot
Match: O24325 (Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 3.5e-105
Identity = 213/479 (44.47%), Postives = 276/479 (57.62%), Query Frame = 0

Query: 1   MATCDGSRTKLVLLIAFLFFNNIVAEREWVSDATAEQS---------GKRWALLVAGSNG 60
           MAT   + + L LL+ FL    + A R+ V D     S         G RWA+L AGS+G
Sbjct: 1   MATTTATTSLLALLLLFL-VALVSAGRDLVGDFLRLPSDSGNGDNVHGTRWAILFAGSSG 60

Query: 61  YDNYRHQADVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGV 120
           Y NYRHQAD+CHAYQ+LRKGGL D+NIIVFMYDDIAF+  NPR GVIIN P+G +VY+GV
Sbjct: 61  YWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVIINSPNGDEVYKGV 120

Query: 121 PKDYTGKHVNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGD 180
           PKDYTG+ V A NFYA +LG+KS LTGGSGKV+NSG +DHIFI+Y+DHG  G+LG P G 
Sbjct: 121 PKDYTGEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGP 180

Query: 181 YIYSKDLIDVLKKNMKPKVTK-------AC------------------------------ 240
           YIY+ DL +VLKK       K       AC                              
Sbjct: 181 YIYASDLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVYATTASNADESSWG 240

Query: 241 ------------------------------DKHDLSKETLNQQYHVVRRRTLVDKFGYGS 300
                                         D+H+L  ETL+QQY +V+ RT+     YGS
Sbjct: 241 TYCPGEDPSPPPEYSTCLGDLYSVAWMEDSDRHNLRTETLHQQYKLVKERTISGGLYYGS 300

Query: 301 HVMLYGNKSIGNSFLNIYIGNSPDNNYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASL 360
           HVM YG+  +    L  Y+G  P N   +FV             SL S++ AV+Q DA L
Sbjct: 301 HVMQYGDVGLSKDILFHYLGTDPANENLTFVDEN----------SLWSSSKAVNQRDADL 360

Query: 361 IHYWYKFQNAPFGSREKMQAREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRR 404
           +H+W KF+ AP GS +K +AR+Q+ + + +R H D S+  +G+LLFG   + E+LN VR 
Sbjct: 361 VHFWDKFRKAPEGSPKKNEARKQVLEVMSHRMHIDDSVELVGKLLFGIEKAPELLNAVRP 420

BLAST of Lag0003228.1 vs. ExPASy Swiss-Prot
Match: P49043 (Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.1e-103
Identity = 203/431 (47.10%), Postives = 261/431 (60.56%), Query Frame = 0

Query: 39  GKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVI 98
           G RWA+L+AGSNG+ NYRHQAD+CHAYQ+LRKGGL D+NIIVFMYDDIAF+  NPRPGVI
Sbjct: 58  GTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNEENPRPGVI 117

Query: 99  INKPDGVDVYQGVPKDYTGKHVNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTD 158
           IN P G DVY+GVPKDYTG+ V    F+AV+LGNK+ALTGGSGKV++SG +DHIFI+Y+D
Sbjct: 118 INHPHGDDVYKGVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVVDSGPNDHIFIFYSD 177

Query: 159 HGAAGMLGMPEGDYIYSKDLIDVLKK-----NMKPKV--TKAC----------------- 218
           HG  G+LGMP   YIY+ +LIDVLKK     N K  V   +AC                 
Sbjct: 178 HGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESGSIFEGLLLEGLNIY 237

Query: 219 -------------------------------------------DKHDLSKETLNQQYHVV 278
                                                      D H+L  ETL+QQY +V
Sbjct: 238 ATTASNAEESSWGTYCPGEIPGPPPEYSTCLGDLYSIAWMEDSDIHNLRTETLHQQYELV 297

Query: 279 RRRTLVDKFGYGSHVMLYGNKSIGNSFLNIYIGNSPDNNYASFVQSAHNISSPSKLLSLS 338
           + RT      YGSHVM YG+  +  + L  Y+G +P N+  +FV             SL 
Sbjct: 298 KTRT-ASYNSYGSHVMQYGDIGLSKNNLFTYLGTNPANDNYTFVDEN----------SLR 357

Query: 339 SNNAAVSQHDASLIHYWYKFQNAPFGSREKMQAREQLEDEILNRRHADYSINHIGELLFG 398
             + AV+Q DA L+H+W K++ AP G+  K +A++Q  + + +R H D+SI  IG+LLFG
Sbjct: 358 PASKAVNQRDADLLHFWDKYRKAPEGTPRKAEAQKQFFEAMSHRMHVDHSIKLIGKLLFG 417

Query: 399 HANSSEVLNNVRRQGQSLIDDWGCFRTFVKTYEKHCKRLSRYGMKYTRALANICNAGITI 403
                E+LN VR  GQ L+DDWGC ++ V+T+E HC  LS+YGMK+ R+LANICN GI  
Sbjct: 418 IEKGPEILNTVRPAGQPLVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGK 477

BLAST of Lag0003228.1 vs. ExPASy Swiss-Prot
Match: Q39119 (Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g32940 PE=1 SV=2)

HSP 1 Score: 374.4 bits (960), Expect = 1.6e-102
Identity = 194/432 (44.91%), Postives = 261/432 (60.42%), Query Frame = 0

Query: 38  SGKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGV 97
           SG RWA+LVAGS+GY NYRHQAD+CHAYQ+LRKGGL ++NI+VFMYDDIA +  NPRPG 
Sbjct: 56  SGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGT 115

Query: 98  IINKPDGVDVYQGVPKDYTGKHVNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYT 157
           IIN P G DVYQGVPKDYTG  VN  N +AVILG+K+A+ GGSGKV++SG +DHIFI+Y+
Sbjct: 116 IINSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYS 175

Query: 158 DHGAAGMLGMPEGDYIYSKDLIDVLKKNMKPKVTK-------ACDK-------------- 217
           DHG  G+LGMP   Y+Y+ DL DVLKK       K       AC+               
Sbjct: 176 DHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNI 235

Query: 218 ----------------------------------------------HDLSKETLNQQYHV 277
                                                         H+L  ETL+QQY +
Sbjct: 236 YATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYEL 295

Query: 278 VRRRTLVDKFGYGSHVMLYGNKSIGNSFLNIYIGNSPDNNYASFVQSAHNISSPSKLLSL 337
           V+RRT    + YGSHVM YG+  I    L++Y+G +P N+  +F   A+++  PS++   
Sbjct: 296 VKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFA-DANSLKPPSRV--- 355

Query: 338 SSNNAAVSQHDASLIHYWYKFQNAPFGSREKMQAREQLEDEILNRRHADYSINHIGELLF 397
                  +Q DA L+H+W K++ AP GS  K +A++Q+ + + +R H D S+  +G++LF
Sbjct: 356 ------TNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILF 415

Query: 398 GHANSSEVLNNVRRQGQSLIDDWGCFRTFVKTYEKHCKRLSRYGMKYTRALANICNAGIT 403
           G +   EVLN VR  GQ L+DDW C +  V+ +E+HC  LS+YG+K+ R+ ANICNAGI 
Sbjct: 416 GISRGPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQ 475

BLAST of Lag0003228.1 vs. ExPASy Swiss-Prot
Match: P49047 (Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g25940 PE=2 SV=2)

HSP 1 Score: 359.8 bits (922), Expect = 4.2e-98
Identity = 188/434 (43.32%), Postives = 260/434 (59.91%), Query Frame = 0

Query: 36  EQSGKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRP 95
           +    +WA+LVAGS+GY NYRHQADVCHAYQ+L+KGG+ ++NI+VFMYDDIA +  NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 96  GVIINKPDGVDVYQGVPKDYTGKHVNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIY 155
           GVIIN P+G DVY GVPKDYTG  VN  N  AVILGNK+AL GGSGKV++SG +DHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 156 YTDHGAAGMLGMPEGDYIYSKDLIDVLKKN------------------------------ 215
           Y+DHG  G+LGMP    +Y+ DL DVLKK                               
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 216 ------------------------MKPKVTKAC-------------DKHDLSKETLNQQY 275
                                     P   + C             +KH+L  ETL++QY
Sbjct: 219 NIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQY 278

Query: 276 HVVRRRTLVDKFGYGSHVMLYGNKSIGNSFLNIYIGNSPDNNYASFVQSAHNISSPSKLL 335
            +V++RT      YGSHVM +G+  +    L +++G +P +   +FV + ++I  PS++ 
Sbjct: 279 ELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFV-NENSIRPPSRV- 338

Query: 336 SLSSNNAAVSQHDASLIHYWYKFQNAPFGSREKMQAREQLEDEILNRRHADYSINHIGEL 395
                    +Q DA L+H+W+K+Q AP GS  K++A++Q+ + + +R H D SI  IG L
Sbjct: 339 --------TNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGIL 398

Query: 396 LFGHANSSEVLNNVRRQGQSLIDDWGCFRTFVKTYEKHCKRLSRYGMKYTRALANICNAG 403
           LFG      VLN VR  G+ L+DDW C ++ V+ +E+HC  LS+YG+K+ R++AN+CNAG
Sbjct: 399 LFG-LEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAG 458

BLAST of Lag0003228.1 vs. ExPASy TrEMBL
Match: A0A6J1D475 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 4.8e-174
Identity = 318/474 (67.09%), Postives = 358/474 (75.53%), Query Frame = 0

Query: 1   MATCDGSRTKLVLLIAFLFFNNIVAEREWVSDATAEQSGKRWALLVAGSNGYDNYRHQAD 60
           MATC  + TK VLLIA L F++I+A+RE V  A+ +Q GKRWA+LVAGS+GY+NYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 61  VCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKHV 120
           VCHAYQILRKGGL D+NIIVFMYDDIAF+PSNPRPGV+INKPDGVDVYQGVPKDYTG+HV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 121 NAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLID 180
           N+INFYAVILGN+SALTGGSGKV++S  HDHIFIYYTDHG+AG+LGMPEGDY+Y+KDL++
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 181 VLKKNMKPKVTK-------AC--------------------------------------- 240
           VLK+  + K  K       AC                                       
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 241 ---------------------DKHDLSKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNKS 300
                                DKHDLSKETL QQY  VRRRTLVDKFGYGSHVMLYGNKS
Sbjct: 241 PPTDYDTCLGDLYSIAWMEDSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKS 300

Query: 301 IGNSFLNIYIGNSPDN-NYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASLIHYWYKFQ 360
           IGN+ L+ YIG +PDN NY S VQS   I+ PSKLL     + AVSQ DASLIHYW+KFQ
Sbjct: 301 IGNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLL----YSNAVSQRDASLIHYWHKFQ 360

Query: 361 NAPFGSREKMQAREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRRQGQSLIDD 407
            APFGSREK +AR+QLEDEILNRRH D SI HI +LLFG A SSEVLNNVR+QGQSL+DD
Sbjct: 361 KAPFGSREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDD 420

BLAST of Lag0003228.1 vs. ExPASy TrEMBL
Match: A0A6J1D2Z4 (vacuolar-processing enzyme-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 4.2e-170
Identity = 318/508 (62.60%), Postives = 358/508 (70.47%), Query Frame = 0

Query: 1   MATCDGSRTKLVLLIAFLFFNNIVAEREWVSDATAEQSGKRWALLVAGSNGYDNYRHQAD 60
           MATC  + TK VLLIA L F++I+A+RE V  A+ +Q GKRWA+LVAGS+GY+NYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 61  VCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKHV 120
           VCHAYQILRKGGL D+NIIVFMYDDIAF+PSNPRPGV+INKPDGVDVYQGVPKDYTG+HV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 121 NAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLID 180
           N+INFYAVILGN+SALTGGSGKV++S  HDHIFIYYTDHG+AG+LGMPEGDY+Y+KDL++
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 181 VLKKNMKPKVTK-------AC--------------------------------------- 240
           VLK+  + K  K       AC                                       
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 241 -------------------------------------------------------DKHDL 300
                                                                  DKHDL
Sbjct: 241 PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL 300

Query: 301 SKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNKSIGNSFLNIYIGNSPDN-NYASFVQSA 360
           SKETL QQY  VRRRTLVDKFGYGSHVMLYGNKSIGN+ L+ YIG +PDN NY S VQS 
Sbjct: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360

Query: 361 HNISSPSKLLSLSSNNAAVSQHDASLIHYWYKFQNAPFGSREKMQAREQLEDEILNRRHA 407
             I+ PSKLL     + AVSQ DASLIHYW+KFQ APFGSREK +AR+QLEDEILNRRH 
Sbjct: 361 DTIAPPSKLL----YSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHV 420

BLAST of Lag0003228.1 vs. ExPASy TrEMBL
Match: A0A5D3DZP1 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00260 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 7.0e-165
Identity = 308/466 (66.09%), Postives = 341/466 (73.18%), Query Frame = 0

Query: 1   MATCDGSRTK-LVLLIAFLFFNNIVAEREWVSDATAEQSGKRWALLVAGSNGYDNYRHQA 60
           M TCD + TK L++LI FL FNNI+A+ EW+SDAT +Q+GK WALL+AGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKH 120
                      GG+ D+NIIVFMYDDIAFHPSNPRPG+IINKPDG DVY GVPKDYTGKH
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLI 180
           VNA+NFYA ILGNKSALTGGSGKVI+SG  DHIFIYY+DHGAAGMLGMPEGDYI+  DLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKKNMKPK-------VTKAC-------------------------------------- 240
           DVLK+  K K         +AC                                      
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 -------------DKHDLSKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNKSIGNSFLNI 300
                        DKHDLSKETLNQQYH VRRRT VDK GYGSHVMLYGNK IGN FL+ 
Sbjct: 241 RSPKDYNTCLGDFDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDT 300

Query: 301 YIGNSPD-NNYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASLIHYWYKFQNAPFGSRE 360
           ++GNSPD +NY   VQS   ISS SK   LSSN  +VSQ DASLIHY YKFQ APFGSRE
Sbjct: 301 FLGNSPDYDNYKYSVQSPQTISSLSK---LSSN--SVSQRDASLIHYQYKFQEAPFGSRE 360

Query: 361 KMQAREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRRQGQSLIDDWGCFRTFV 407
           KM+AREQLEDEI  RRHAD SIN IG+LLFG A SSE+L+NVR QGQSL+DDW CF+TFV
Sbjct: 361 KMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFV 420

BLAST of Lag0003228.1 vs. ExPASy TrEMBL
Match: A0A5A7TXA7 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00680 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.7e-164
Identity = 308/475 (64.84%), Postives = 341/475 (71.79%), Query Frame = 0

Query: 1   MATCDGSRTK-LVLLIAFLFFNNIVAEREWVSDATAEQSGKRWALLVAGSNGYDNYRHQA 60
           M TCD + TK L++LI FL FNNI+A+ EW+SDAT +Q+GK WALL+AGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKH 120
                      GG+ D+NIIVFMYDDIAFHPSNPRPG+IINKPDG DVY GVPKDYTGKH
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLI 180
           VNA+NFYA ILGNKSALTGGSGKVI+SG  DHIFIYY+DHGAAGMLGMPEGDYI+  DLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKKNMKPK-------VTKAC-------------------------------------- 240
           DVLK+  K K         +AC                                      
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 ----------------------DKHDLSKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNK 300
                                 DKHDLSKETLNQQYH VRRRT VDK GYGSHVMLYGNK
Sbjct: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300

Query: 301 SIGNSFLNIYIGNSPD-NNYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASLIHYWYKF 360
            IGN FL+ ++GNSPD +NY   VQS   ISS SK   LSSN  +VSQ DASLIHY YKF
Sbjct: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSK---LSSN--SVSQRDASLIHYQYKF 360

Query: 361 QNAPFGSREKMQAREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRRQGQSLID 407
           Q APFGSREKM+AREQLEDEI  RRHAD SIN IG+LLFG A SSE+L+NVR QGQSL+D
Sbjct: 361 QEAPFGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVD 420

BLAST of Lag0003228.1 vs. ExPASy TrEMBL
Match: A0A6J1HBF5 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=LOC111461846 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.9e-162
Identity = 298/463 (64.36%), Postives = 334/463 (72.14%), Query Frame = 0

Query: 12  VLLIAFLFFNNIVAEREWVSDATAEQSGKRWALLVAGSNGYDNYRHQADVCHAYQILRKG 71
           +LLIA LFFN+I+A+++WVS A  +Q GKRWALLVAGSNGYDNYRHQADVCHAYQILRK 
Sbjct: 29  LLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRK- 88

Query: 72  GLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKHVNAINFYAVILG 131
           G+ D+NIIVFMYDDIA HP NPRPG+IINKP G DVYQGVPKDYTGK VN++NFYA ILG
Sbjct: 89  GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILG 148

Query: 132 NKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLIDVLKKNMKPKVT 191
           NKS +TGGSGKVI+SG +DHIFIYYTDHGAAGMLGMP GDYIYS DL+DVLK+  + K  
Sbjct: 149 NKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSY 208

Query: 192 K-------AC-------------------------------------------------- 251
           K       AC                                                  
Sbjct: 209 KSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGD 268

Query: 252 ----------DKHDLSKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNKSIGNSFLNIYIG 311
                     DKHD SKETLNQQYH VRRRT VDK GYGSHVMLYGN+SIGN+FLN Y G
Sbjct: 269 LYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIGNNFLNTYFG 328

Query: 312 N-SPDNNYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASLIHYWYKFQNAPFGSREKMQ 371
           N S DNN  S V+S H ISS S           VSQ DASL+HYWYKFQNAP+GSREKM+
Sbjct: 329 NTSDDNNNESSVKSTHIISSLSN---------GVSQRDASLLHYWYKFQNAPYGSREKME 388

Query: 372 AREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRRQGQSLIDDWGCFRTFVKTY 407
           A E+LEDEIL+RRHAD S+N IGELLFG A SS VLN +R  GQS +DDW CF+ +VK Y
Sbjct: 389 ASERLEDEILSRRHADNSVNQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 448

BLAST of Lag0003228.1 vs. TAIR 10
Match: AT4G32940.1 (gamma vacuolar processing enzyme )

HSP 1 Score: 374.4 bits (960), Expect = 1.2e-103
Identity = 194/432 (44.91%), Postives = 261/432 (60.42%), Query Frame = 0

Query: 38  SGKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRPGV 97
           SG RWA+LVAGS+GY NYRHQAD+CHAYQ+LRKGGL ++NI+VFMYDDIA +  NPRPG 
Sbjct: 56  SGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGT 115

Query: 98  IINKPDGVDVYQGVPKDYTGKHVNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIYYT 157
           IIN P G DVYQGVPKDYTG  VN  N +AVILG+K+A+ GGSGKV++SG +DHIFI+Y+
Sbjct: 116 IINSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYS 175

Query: 158 DHGAAGMLGMPEGDYIYSKDLIDVLKKNMKPKVTK-------ACDK-------------- 217
           DHG  G+LGMP   Y+Y+ DL DVLKK       K       AC+               
Sbjct: 176 DHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNI 235

Query: 218 ----------------------------------------------HDLSKETLNQQYHV 277
                                                         H+L  ETL+QQY +
Sbjct: 236 YATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYEL 295

Query: 278 VRRRTLVDKFGYGSHVMLYGNKSIGNSFLNIYIGNSPDNNYASFVQSAHNISSPSKLLSL 337
           V+RRT    + YGSHVM YG+  I    L++Y+G +P N+  +F   A+++  PS++   
Sbjct: 296 VKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFA-DANSLKPPSRV--- 355

Query: 338 SSNNAAVSQHDASLIHYWYKFQNAPFGSREKMQAREQLEDEILNRRHADYSINHIGELLF 397
                  +Q DA L+H+W K++ AP GS  K +A++Q+ + + +R H D S+  +G++LF
Sbjct: 356 ------TNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILF 415

Query: 398 GHANSSEVLNNVRRQGQSLIDDWGCFRTFVKTYEKHCKRLSRYGMKYTRALANICNAGIT 403
           G +   EVLN VR  GQ L+DDW C +  V+ +E+HC  LS+YG+K+ R+ ANICNAGI 
Sbjct: 416 GISRGPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQ 475

BLAST of Lag0003228.1 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 359.8 bits (922), Expect = 3.0e-99
Identity = 188/434 (43.32%), Postives = 260/434 (59.91%), Query Frame = 0

Query: 36  EQSGKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLSDDNIIVFMYDDIAFHPSNPRP 95
           +    +WA+LVAGS+GY NYRHQADVCHAYQ+L+KGG+ ++NI+VFMYDDIA +  NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 96  GVIINKPDGVDVYQGVPKDYTGKHVNAINFYAVILGNKSALTGGSGKVINSGSHDHIFIY 155
           GVIIN P+G DVY GVPKDYTG  VN  N  AVILGNK+AL GGSGKV++SG +DHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 156 YTDHGAAGMLGMPEGDYIYSKDLIDVLKKN------------------------------ 215
           Y+DHG  G+LGMP    +Y+ DL DVLKK                               
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 216 ------------------------MKPKVTKAC-------------DKHDLSKETLNQQY 275
                                     P   + C             +KH+L  ETL++QY
Sbjct: 219 NIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQY 278

Query: 276 HVVRRRTLVDKFGYGSHVMLYGNKSIGNSFLNIYIGNSPDNNYASFVQSAHNISSPSKLL 335
            +V++RT      YGSHVM +G+  +    L +++G +P +   +FV + ++I  PS++ 
Sbjct: 279 ELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFV-NENSIRPPSRV- 338

Query: 336 SLSSNNAAVSQHDASLIHYWYKFQNAPFGSREKMQAREQLEDEILNRRHADYSINHIGEL 395
                    +Q DA L+H+W+K+Q AP GS  K++A++Q+ + + +R H D SI  IG L
Sbjct: 339 --------TNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGIL 398

Query: 396 LFGHANSSEVLNNVRRQGQSLIDDWGCFRTFVKTYEKHCKRLSRYGMKYTRALANICNAG 403
           LFG      VLN VR  G+ L+DDW C ++ V+ +E+HC  LS+YG+K+ R++AN+CNAG
Sbjct: 399 LFG-LEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAG 458

BLAST of Lag0003228.1 vs. TAIR 10
Match: AT3G20210.1 (delta vacuolar processing enzyme )

HSP 1 Score: 353.2 bits (905), Expect = 2.8e-97
Identity = 203/464 (43.75%), Postives = 259/464 (55.82%), Query Frame = 0

Query: 11  LVLLIAFLFFNNIVAEREWVSDATAEQS-----GKRWALLVAGSNGYDNYRHQADVCHAY 70
           LV L A L F+    + + ++D   E S     G RWA+LVAGSN Y NYRHQAD+CHAY
Sbjct: 11  LVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAY 70

Query: 71  QILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKHVNAINF 130
           QILRKGGL D+NIIVFMYDDIAF   NPRPGVIINKPDG DVY+GVPKDYT + VN  NF
Sbjct: 71  QILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNF 130

Query: 131 YAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLIDVLKKN 190
           Y V+LGN+S +TGG+GKV+ SG +D+IFIYY DHGA G++ MP GD + +KD  +VL+K 
Sbjct: 131 YNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKM 190

Query: 191 MKPK-------VTKAC-------------------------------------------- 250
            K K         +AC                                            
Sbjct: 191 HKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEI 250

Query: 251 ----------------DKHDLSKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNKSIGNSF 310
                           D HD+SKETL QQYHVV+RR   D     SHV  +G + +   +
Sbjct: 251 GTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSD-VPETSHVCRFGTEKMLKDY 310

Query: 311 LNIYIGNSPDNNYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASLIHYWYKFQNAPFGS 370
           L+ YIG +P+N+  +F +S    SSP       SN+  V+  D  L++   K Q AP GS
Sbjct: 311 LSSYIGRNPENDNFTFTES---FSSP------ISNSGLVNPRDIPLLYLQRKIQKAPMGS 370

Query: 371 REKMQAREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRRQGQSLIDDWGCFRT 403
            E  +A+++L DE  +R+  D SI  I  L     N   +L + R  GQ L+DDW CF+T
Sbjct: 371 LESKEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKT 430

BLAST of Lag0003228.1 vs. TAIR 10
Match: AT3G20210.2 (delta vacuolar processing enzyme )

HSP 1 Score: 350.1 bits (897), Expect = 2.3e-96
Identity = 202/462 (43.72%), Postives = 258/462 (55.84%), Query Frame = 0

Query: 11  LVLLIAFLFFNNIVAEREWVSDATAEQS-----GKRWALLVAGSNGYDNYRHQADVCHAY 70
           LV L A L F+    + + ++D   E S     G RWA+LVAGSN Y NYRHQAD+CHAY
Sbjct: 11  LVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAY 70

Query: 71  QILRKGGLSDDNIIVFMYDDIAFHPSNPRPGVIINKPDGVDVYQGVPKDYTGKHVNAINF 130
           QILRKGGL D+NIIVFMYDDIAF   NPRPGVIINKPDG DVY+GVPKDYT + VN  NF
Sbjct: 71  QILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNF 130

Query: 131 YAVILGNKSALTGGSGKVINSGSHDHIFIYYTDHGAAGMLGMPEGDYIYSKDLIDVLKKN 190
           Y V+LGN+S +TGG+GKV+ SG +D+IFIYY DHGA G++ MP GD + +KD  +VL+K 
Sbjct: 131 YNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKM 190

Query: 191 MKPK-------VTKAC-------------------------------------------- 250
            K K         +AC                                            
Sbjct: 191 HKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEI 250

Query: 251 ----------------DKHDLSKETLNQQYHVVRRRTLVDKFGYGSHVMLYGNKSIGNSF 310
                           D HD+SKETL QQYHVV+RR   D     SHV  +G + +   +
Sbjct: 251 GTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSD-VPETSHVCRFGTEKMLKDY 310

Query: 311 LNIYIGNSPDNNYASFVQSAHNISSPSKLLSLSSNNAAVSQHDASLIHYWYKFQNAPFGS 370
           L+ YIG +P+N+  +F +S    SSP       SN+  V+  D  L++   K Q AP GS
Sbjct: 311 LSSYIGRNPENDNFTFTES---FSSP------ISNSGLVNPRDIPLLYLQRKIQKAPMGS 370

Query: 371 REKMQAREQLEDEILNRRHADYSINHIGELLFGHANSSEVLNNVRRQGQSLIDDWGCFRT 401
            E  +A+++L DE  +R+  D SI  I  L     N   +L + R  GQ L+DDW CF+T
Sbjct: 371 LESKEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKT 430

BLAST of Lag0003228.1 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 337.8 bits (865), Expect = 1.2e-92
Identity = 187/442 (42.31%), Postives = 246/442 (55.66%), Query Frame = 0

Query: 31  SDATAEQSGKRWALLVAGSNGYDNYRHQADVCHAYQILRKGGLSDDNIIVFMYDDIAFHP 90
           +D   +  G RWA+LVAGS+GY NYRHQADVCHAYQILRKGGL ++NI+V MYDDIA HP
Sbjct: 41  ADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHP 100

Query: 91  SNPRPGVIINKPDGVDVYQGVPKDYTGKHVNAINFYAVILGNKSALTGGSGKVINSGSHD 150
            NPRPG +IN PDG DVY GVPKDYTG  V A NFYAV+LG++ A+ GGSGKVI S  +D
Sbjct: 101 LNPRPGTLINHPDGDDVYAGVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPND 160

Query: 151 HIFIYYTDHGAAGMLGMPEGDYIYSKDLIDVLKK------------------------NM 210
           HIF+YY DHG  G+LGMP   +IY+ D I+ LKK                         +
Sbjct: 161 HIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGI 220

Query: 211 KPK-----VTKA--------------------------------------CDKHDLSKET 270
            PK     VT A                                       + H+L KET
Sbjct: 221 MPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSETHNLKKET 280

Query: 271 LNQQYHVVRRRTL-VDKFGYGSHVMLYGNKSIGNSFLNIYIGNSPDNNYASFVQSAHNIS 330
           + QQYH V+ RT   + +  GSHVM YGN SI +  L +Y G  P             ++
Sbjct: 281 IKQQYHTVKMRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGFDP-----------ATVN 340

Query: 331 SPSKLLSLSSNNAAVSQHDASLIHYWYKFQNAPFGSREKMQAREQLEDEILNRRHADYSI 390
            P   L + S    V+Q DA L+  W+ ++ +  GSR+K    ++L +   +R+H D S+
Sbjct: 341 LPLNELPVKSKIGVVNQRDADLLFLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLDASV 400

Query: 391 NHIGELLFGHANSSEVLNNVRRQGQSLIDDWGCFRTFVKTYEKHCKRLSRYGMKYTRALA 405
             I  +LFG   +  VLN VR  G  L+DDW C ++ V+ +E+HC  L++YGMK+ RA A
Sbjct: 401 ELIATILFG--PTMNVLNLVREPGLPLVDDWECLKSMVRVFEEHCGSLTQYGMKHMRAFA 460