IVF0021004.2 (mRNA) Melon (IVF77) v1

Overview


Name	IVF0021004.2
Type	mRNA
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	calcium-binding allergen Bet v 3
Location	chr04: 32176072 .. 32176670 (-)
Sequence length	549
RNA-Seq Expression	IVF0021004.2
Synteny	IVF0021004.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCGGTGGCCTCTGATGTTCCCCGACCCCGGCTGACGAAGAAACCCTCTTCTTCGTCTTCGTCGTTCAGGCTTCGGTGCCCGAGCCTGAACTCCCTTCGCCTGCGTCGCATATTGACATGTTCGACAAGAACGGGGACGGGATGATAACGGCGGAGGAGCTGGGTCAAGCTCTGGGGCTTCTTGGGTTGGATGCTGATGAATCGGAGCTGGATTCGACGGTCAACTCCTTCATAAAACCGGGAAATGTGGGTCTTAGGTTCGAAGACTTCGCAGCCCTGCACCAGTCGCTAAATGACACGTATTTTGCGGGAATGGTGGAGGATGAGGATGAGAAGAGGGAAACAAGTATGTCGTCGATGTCGCAGGAAGAATCGGATCTATCGGAGGCATTCAGGGTATTCGACGAGGATGGAGATGGCTACATATCCGCCACCGAACTCCAAGTGGTTCTAAGAAAGCTGAGATTGGCGGAGGGGAATGAGATCGACAGGGTTCAGAAGATGATTTTGTCGGTTGACCGCAATCAGGATGGCCGGGTTGATTTCTTCGAATTCAAGGACATGATGCGCAGTGTTATCGTCCGGAGTTGTTGA

mRNA sequence

ATGGAAGCGGTGGCCTCTGATGTTCCCCGACCCCGGCTGACGAAGAAACCCTCTTCTTCGTCTTCGTCGTTCAGGCTTCGGTGCCCGAGCCTGAACTCCCTTCGCCTGCGTCGCATATTGACATCTCTGGGGCTTCTTGGGTTGGATGCTGATGAATCGGAGCTGGATTCGACGGTCAACTCCTTCATAAAACCGGGAAATGTGGGTCTTAGGTTCGAAGACTTCGCAGCCCTGCACCAGTCGCTAAATGACACGTATTTTGCGGGAATGGTGGAGGATGAGGATGAGAAGAGGGAAACAAGTATGTCGTCGATGTCGCAGGAAGAATCGGATCTATCGGAGGCATTCAGGGTATTCGACGAGGATGGAGATGGCTACATATCCGCCACCGAACTCCAAGTGGTTCTAAGAAAGCTGAGATTGGCGGAGGGGAATGAGATCGACAGGGTTCAGAAGATGATTTTGTCGGTTGACCGCAATCAGGATGGCCGGGTTGATTTCTTCGAATTCAAGGACATGATGCGCAGTGTTATCGTCCGGAGTTGTTGA

Coding sequence (CDS)

ATGGAAGCGGTGGCCTCTGATGTTCCCCGACCCCGGCTGACGAAGAAACCCTCTTCTTCGTCTTCGTCGTTCAGGCTTCGGTGCCCGAGCCTGAACTCCCTTCGCCTGCGTCGCATATTGACATCTCTGGGGCTTCTTGGGTTGGATGCTGATGAATCGGAGCTGGATTCGACGGTCAACTCCTTCATAAAACCGGGAAATGTGGGTCTTAGGTTCGAAGACTTCGCAGCCCTGCACCAGTCGCTAAATGACACGTATTTTGCGGGAATGGTGGAGGATGAGGATGAGAAGAGGGAAACAAGTATGTCGTCGATGTCGCAGGAAGAATCGGATCTATCGGAGGCATTCAGGGTATTCGACGAGGATGGAGATGGCTACATATCCGCCACCGAACTCCAAGTGGTTCTAAGAAAGCTGAGATTGGCGGAGGGGAATGAGATCGACAGGGTTCAGAAGATGATTTTGTCGGTTGACCGCAATCAGGATGGCCGGGTTGATTTCTTCGAATTCAAGGACATGATGCGCAGTGTTATCGTCCGGAGTTGTTGA

Protein sequence

MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRILTSLGLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSVIVRSC

Homology

[Close]

BLAST of IVF0021004.2 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.9e-50
Identity = 114/185 (61.62%), Postives = 136/185 (73.51%), Query Frame = 0


Query: 15  KKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSLGLLGLDADESELDS 74
           +K S S+SSFRLR  SLN+LRLRRI                   +L LLGL+ D SEL+S
Sbjct: 20  RKRSLSNSSFRLRSESLNTLRLRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELES 79

Query: 75  TVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMSS-MSQEESDLSEAF 134
           TV SF + GN+GL+FEDF +LHQSLND+YFA   EDED+  E    S +SQEE+D    F
Sbjct: 80  TVKSFTREGNIGLQFEDFISLHQSLNDSYFAYGGEDEDDNEEDMRKSILSQEEADSFGGF 139

Query: 135 RVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRS 182
           +VFDEDGDGYISA ELQ+VL KL  +EG+EIDRV+KMI+SVD N+DGRVDFFEFKDMMRS
Sbjct: 140 KVFDEDGDGYISARELQMVLGKLGFSEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMMRS 199

[Close]

BLAST of IVF0021004.2 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 4.8e-46
Identity = 104/187 (55.61%), Postives = 137/187 (73.26%), Query Frame = 0


Query: 15  KKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSLGLLGLDADESELDS 74
           KK +  SSSFRLR PSLN+LRL+RI                   +L  LGL+AD S+L S
Sbjct: 9   KKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKS 68

Query: 75  TVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMV---EDEDEKRETSMSSMSQEESDLSE 134
           TV S+I+PGN GL F+DF++LH++L+D++F G     E+ED+      SS ++ ESDL+E
Sbjct: 69  TVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDP-----SSAAENESDLAE 128

Query: 135 AFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMM 182
           AF+VFDE+GDG+ISA ELQ VL+KL L EG E++RV+KMI+SVDRNQDGRVDFFEFK+MM
Sbjct: 129 AFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 188

[Close]

BLAST of IVF0021004.2 vs. ExPASy Swiss-Prot
Match: Q09011 (Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 5.3e-45
Identity = 108/184 (58.70%), Postives = 125/184 (67.93%), Query Frame = 0


Query: 15  KKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSLGLLGLDADESELDS 74
           K   SSSSSFRLR PSLNS+RLRRI                   +L LLGLDAD SE++S
Sbjct: 20  KLKPSSSSSFRLRSPSLNSIRLRRIFDVFDRNHDCLISVEELSQALNLLGLDADLSEIES 79

Query: 75  TVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMSSMSQEESDLSEAFR 134
            V   IKP N GLRFEDF  LH+SLND +F    ED    +       +Q+ESDL EAF 
Sbjct: 80  MVKLHIKPENTGLRFEDFETLHRSLNDVFFGSKCED----KLGLNPDPAQDESDLKEAFD 139

Query: 135 VFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSV 182
           VFDE+GDG+ISA ELQVVL KL L EG+EIDRV+ MI SV+++ DGRVDFFEFKDMMR+V
Sbjct: 140 VFDENGDGFISAKELQVVLEKLGLPEGSEIDRVEMMISSVEQDHDGRVDFFEFKDMMRTV 199

[Close]

BLAST of IVF0021004.2 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 1.9e-42
Identity = 103/184 (55.98%), Postives = 125/184 (67.93%), Query Frame = 0


Query: 15  KKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSLGLLGLDADESELDS 74
           KK S  SSSFRLR PSLN+LRL R+                   +L  LGLDAD S+L S
Sbjct: 8   KKLSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKS 67

Query: 75  TVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMSSMSQEESDLSEAFR 134
           TV+SFIKP   GLRF+DFAALH++L++++F G         E S    S  ESDL EAF 
Sbjct: 68  TVDSFIKPDKTGLRFDDFAALHKTLDESFFGG---------EGSCCDGS-PESDLEEAFN 127

Query: 135 VFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSV 182
           VFDEDGDG+ISA ELQ VL+KL L E  EI++V+KMI+SVD N DGRVDFFEFK+MM++V
Sbjct: 128 VFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQTV 181

[Close]

BLAST of IVF0021004.2 vs. ExPASy Swiss-Prot
Match: Q10LX4 (Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 GN=CML27 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 4.2e-34
Identity = 91/187 (48.66%), Postives = 115/187 (61.50%), Query Frame = 0


Query: 10  RPRLTKKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSLGLLGLDADE 69
           +P L++KP   S SFRLR  SLN+LRLRR+                  ++L  LGL AD 
Sbjct: 9   KPSLSRKP---SPSFRLRNGSLNALRLRRVFDLFDRNGDGEITLDEMASALDALGLGADR 68

Query: 70  SELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMSSMSQEESDL 129
           + L++TV  +I  G  GLRF DF ALH++L D  F G VE+E    E        +E D+
Sbjct: 69  AGLEATVGGYIPAGAAGLRFGDFEALHRALGDALF-GPVEEE----EPGKQGEDDDEGDM 128

Query: 130 SEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKD 180
            EAFRVFDEDGDG+ISA ELQ VL+KL L E   +  VQ+MI +VDR+ DGRVDF EFK 
Sbjct: 129 KEAFRVFDEDGDGFISAAELQAVLKKLGLPEARNLATVQEMICNVDRDCDGRVDFGEFKC 187

[Close]

BLAST of IVF0021004.2 vs. ExPASy TrEMBL
Match: A0A5A7TQ56 (Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00090 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.4e-87
Identity = 179/199 (89.95%), Postives = 180/199 (90.45%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSL 60
           MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRI                   +L
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS 120
           GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS 120

Query: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180
           SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG
Sbjct: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180

Query: 181 RVDFFEFKDMMRSVIVRSC 183
           RVDFFEFKDMMRSVIVRSC
Sbjct: 181 RVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of IVF0021004.2 vs. ExPASy TrEMBL
Match: A0A1S3B620 (calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.4e-87
Identity = 179/199 (89.95%), Postives = 180/199 (90.45%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSL 60
           MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRI                   +L
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS 120
           GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS 120

Query: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180
           SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG
Sbjct: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180

Query: 181 RVDFFEFKDMMRSVIVRSC 183
           RVDFFEFKDMMRSVIVRSC
Sbjct: 181 RVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of IVF0021004.2 vs. ExPASy TrEMBL
Match: A0A0A0LA10 (Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.3e-83
Identity = 175/201 (87.06%), Postives = 177/201 (88.06%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSL 60
           MEAVASDVPRPRLTKKPSS SSSFRLRCPSLNSLRLRRI                   +L
Sbjct: 1   MEAVASDVPRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMV--EDEDEKRETS 120
           GLLGLDADESELDSTVNSFI+PGN GLRFEDFAALHQSLNDTYF GMV  EDEDEKRETS
Sbjct: 61  GLLGLDADESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFGGMVEDEDEDEKRETS 120

Query: 121 MSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ 180
           MSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ
Sbjct: 121 MSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ 180

Query: 181 DGRVDFFEFKDMMRSVIVRSC 183
           DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 DGRVDFFEFKDMMRSVIVRSC 201

[Close]

BLAST of IVF0021004.2 vs. ExPASy TrEMBL
Match: A0A6J1J521 (calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC111481430 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.2e-76
Identity = 162/202 (80.20%), Postives = 172/202 (85.15%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSL 60
           MEA+ASDVPRPRLTKKPSSSS SFRLRCPSLNSLRLRRI                   +L
Sbjct: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDE---DEKRET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQSLNDTYF   +EDE   +EKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 183
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of IVF0021004.2 vs. ExPASy TrEMBL
Match: A0A6J1F9I1 (calcium-binding allergen Bet v 3-like OS=Cucurbita moschata OX=3662 GN=LOC111442046 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.6e-76
Identity = 163/202 (80.69%), Postives = 171/202 (84.65%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSL 60
           MEAVASDVPRPRLTKKPSSSS SFRLRCPSLNSLRLRRI                   +L
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDE---DEKRET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQ LNDTYF   VEDE   +EKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQFLNDTYFREPVEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 183
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of IVF0021004.2 vs. NCBI nr
Match: XP_008442311.1 (PREDICTED: calcium-binding allergen Bet v 3 [Cucumis melo] >KAA0044236.1 calcium-binding allergen Bet v 3 [Cucumis melo var. makuwa] >TYK24898.1 calcium-binding allergen Bet v 3 [Cucumis melo var. makuwa])

HSP 1 Score: 332 bits (850), Expect = 8.23e-114
Identity = 179/199 (89.95%), Postives = 180/199 (90.45%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRILT-----------------SL 60
           MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRI                   +L
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS 120
           GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS 120

Query: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180
           SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG
Sbjct: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180

Query: 181 RVDFFEFKDMMRSVIVRSC 182
           RVDFFEFKDMMRSVIVRSC
Sbjct: 181 RVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of IVF0021004.2 vs. NCBI nr
Match: XP_004137668.1 (calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein Csa_002212 [Cucumis sativus])

HSP 1 Score: 319 bits (817), Expect = 9.44e-109
Identity = 175/201 (87.06%), Postives = 177/201 (88.06%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRILT-----------------SL 60
           MEAVASDVPRPRLTKKPSS SSSFRLRCPSLNSLRLRRI                   +L
Sbjct: 1   MEAVASDVPRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDE--KRETS 120
           GLLGLDADESELDSTVNSFI+PGN GLRFEDFAALHQSLNDTYF GMVEDEDE  KRETS
Sbjct: 61  GLLGLDADESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFGGMVEDEDEDEKRETS 120

Query: 121 MSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ 180
           MSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ
Sbjct: 121 MSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ 180

Query: 181 DGRVDFFEFKDMMRSVIVRSC 182
           DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 DGRVDFFEFKDMMRSVIVRSC 201

[Close]

BLAST of IVF0021004.2 vs. NCBI nr
Match: XP_038905011.1 (calcium-binding protein CML42 [Benincasa hispida])

HSP 1 Score: 303 bits (777), Expect = 1.17e-102
Identity = 166/201 (82.59%), Postives = 171/201 (85.07%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRILT-----------------SL 60
           MEAV SDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRI                   +L
Sbjct: 1   MEAVPSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTKELEQAL 60

Query: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDE--KRETS 120
           GLLGLD DES+LD TV SF++PGN GLRFEDFAALHQ LNDTY  GMVEDEDE  KRETS
Sbjct: 61  GLLGLDVDESDLDLTVKSFVQPGNAGLRFEDFAALHQFLNDTYLEGMVEDEDEEEKRETS 120

Query: 121 MSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ 180
           MSSMSQEESDL+EAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ
Sbjct: 121 MSSMSQEESDLTEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ 180

Query: 181 DGRVDFFEFKDMMRSVIVRSC 182
           DG VDFFEFKDMMRSVIVRSC
Sbjct: 181 DGLVDFFEFKDMMRSVIVRSC 201

[Close]

BLAST of IVF0021004.2 vs. NCBI nr
Match: KAG6580420.1 (Calcium-binding allergen Bet v 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 298 bits (764), Expect = 1.16e-100
Identity = 164/202 (81.19%), Postives = 172/202 (85.15%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRILT-----------------SL 60
           MEAVASDVPRPRLTKKPSSSS SFRLRCPSLNSLRLRRI                   +L
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEK---RET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQSLNDTYF   VEDE+EK   RET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFREPVEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 182
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of IVF0021004.2 vs. NCBI nr
Match: XP_022982604.1 (calcium-binding allergen Bet v 3-like [Cucurbita maxima])

HSP 1 Score: 296 bits (759), Expect = 6.70e-100
Identity = 162/202 (80.20%), Postives = 172/202 (85.15%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRILT-----------------SL 60
           MEA+ASDVPRPRLTKKPSSSS SFRLRCPSLNSLRLRRI                   +L
Sbjct: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEK---RET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQSLNDTYF   +EDE+EK   RET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 182
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of IVF0021004.2 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 185.7 bits (470), Expect = 3.4e-47
Identity = 104/187 (55.61%), Postives = 137/187 (73.26%), Query Frame = 0


Query: 15  KKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSLGLLGLDADESELDS 74
           KK +  SSSFRLR PSLN+LRL+RI                   +L  LGL+AD S+L S
Sbjct: 9   KKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKS 68

Query: 75  TVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMV---EDEDEKRETSMSSMSQEESDLSE 134
           TV S+I+PGN GL F+DF++LH++L+D++F G     E+ED+      SS ++ ESDL+E
Sbjct: 69  TVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDP-----SSAAENESDLAE 128

Query: 135 AFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMM 182
           AF+VFDE+GDG+ISA ELQ VL+KL L EG E++RV+KMI+SVDRNQDGRVDFFEFK+MM
Sbjct: 129 AFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 188

[Close]

BLAST of IVF0021004.2 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 173.7 bits (439), Expect = 1.3e-43
Identity = 103/184 (55.98%), Postives = 125/184 (67.93%), Query Frame = 0


Query: 15  KKPSSSSSSFRLRCPSLNSLRLRRIL-----------------TSLGLLGLDADESELDS 74
           KK S  SSSFRLR PSLN+LRL R+                   +L  LGLDAD S+L S
Sbjct: 8   KKLSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKS 67

Query: 75  TVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMSSMSQEESDLSEAFR 134
           TV+SFIKP   GLRF+DFAALH++L++++F G         E S    S  ESDL EAF 
Sbjct: 68  TVDSFIKPDKTGLRFDDFAALHKTLDESFFGG---------EGSCCDGS-PESDLEEAFN 127

Query: 135 VFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSV 182
           VFDEDGDG+ISA ELQ VL+KL L E  EI++V+KMI+SVD N DGRVDFFEFK+MM++V
Sbjct: 128 VFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQTV 181

[Close]

BLAST of IVF0021004.2 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 80.9 bits (198), Expect = 1.2e-15
Identity = 48/130 (36.92%), Postives = 72/130 (55.38%), Query Frame = 0


Query: 46  LGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMSSM 105
           LG+   E EL   +      G+  +  E+F  L++++       MVEDEDE         
Sbjct: 33  LGIIIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTI-------MVEDEDE--------- 92

Query: 106 SQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRV 165
              E D+ EAF VFD +GDG+I+  EL+ VL  L L +G  ++  +KMI+ VD + DGRV
Sbjct: 93  -VGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRV 145

Query: 166 DFFEFKDMMR 176
           ++ EF+ MM+
Sbjct: 153 NYMEFRQMMK 145

[Close]

BLAST of IVF0021004.2 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 75.1 bits (183), Expect = 6.5e-14
Identity = 45/130 (34.62%), Postives = 73/130 (56.15%), Query Frame = 0


Query: 46  LGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMSSM 105
           +G+   E+E++  +      G+  +  ++F +L+Q         MVE++           
Sbjct: 33  VGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQE--------MVEEK----------- 92

Query: 106 SQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRV 165
            +EE D+ EAFRVFD++GDG+I+  EL+ VL  + L +G  ++  +KMI  VD + DG V
Sbjct: 93  -EEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDGDGMV 142

Query: 166 DFFEFKDMMR 176
           +F EFK MMR
Sbjct: 153 NFKEFKQMMR 142

[Close]

BLAST of IVF0021004.2 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 74.7 bits (182), Expect = 8.5e-14
Identity = 46/139 (33.09%), Postives = 75/139 (53.96%), Query Frame = 0


Query: 37  RRILTSLGLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDE 96
           + +  +L  LG+   + EL   +      G+  +  ++F  L++++ D        +EDE
Sbjct: 24  KELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGELYKTIMD--------EEDE 83

Query: 97  KRETSMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILS 156
                      EE D+ EAF VFD++GDG+I+  EL+ VL  L L +G  +D  +KMI  
Sbjct: 84  -----------EEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKK 143

Query: 157 VDRNQDGRVDFFEFKDMMR 176
           VD + DGRV++ EF+ MM+
Sbjct: 144 VDVDGDGRVNYKEFRQMMK 143

The following BLAST results are available for this feature:

BLAST of IVF0021004.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43187	1.9e-50	61.62	Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1	[more]
Q9SVG9	4.8e-46	55.61	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q09011	5.3e-45	58.70	Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q9FI19	1.9e-42	55.98	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q10LX4	4.2e-34	48.66	Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of IVF0021004.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TQ56	3.4e-87	89.95	Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B620	3.4e-87	89.95	calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV...	[more]
A0A0A0LA10	2.3e-83	87.06	Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=...	[more]
A0A6J1J521	1.2e-76	80.20	calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC11148143...	[more]
A0A6J1F9I1	1.6e-76	80.69	calcium-binding allergen Bet v 3-like OS=Cucurbita moschata OX=3662 GN=LOC111442...	[more]

BLAST of IVF0021004.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442311.1	8.23e-114	89.95	PREDICTED: calcium-binding allergen Bet v 3 [Cucumis melo] >KAA0044236.1 calcium...	[more]
XP_004137668.1	9.44e-109	87.06	calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein...	[more]
XP_038905011.1	1.17e-102	82.59	calcium-binding protein CML42 [Benincasa hispida]	[more]
KAG6580420.1	1.16e-100	81.19	Calcium-binding allergen Bet v 3, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022982604.1	6.70e-100	80.20	calcium-binding allergen Bet v 3-like [Cucurbita maxima]	[more]

BLAST of IVF0021004.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20780.1	3.4e-47	55.61	calmodulin like 42	[more]
AT5G44460.1	1.3e-43	55.98	calmodulin like 43	[more]
AT4G03290.1	1.2e-15	36.92	EF hand calcium-binding protein family	[more]
AT4G12860.1	6.5e-14	34.62	EF hand calcium-binding protein family	[more]
AT1G05990.1	8.5e-14	33.09	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 111..139 e-value: 2.8E-6 score: 36.9 coord: 149..177 e-value: 9.0E-6 score: 35.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 110..175 e-value: 3.2E-13 score: 49.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 145..180 score: 13.592857
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 107..142 score: 14.90394
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 111..175 e-value: 6.07704E-17 score: 69.1137
None	No IPR available	GENE3D	1.10.238.10		coord: 28..177 e-value: 1.4E-22 score: 82.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR23050:SF356	CALCIUM BINDING	coord: 38..181
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 38..181
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 158..170
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 120..132
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 36..175

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
IVF0021004	IVF0021004	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
IVF0021004.2-exon	IVF0021004.2-exon-chr04:32176072..32176496	exon
IVF0021004.2-exon	IVF0021004.2-exon-chr04:32176547..32176670	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
IVF0021004.2-cds	IVF0021004.2-cds-chr04:32176072..32176496	CDS
IVF0021004.2-cds	IVF0021004.2-cds-chr04:32176547..32176670	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
IVF0021004.2	IVF0021004.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding