IVF0001843.1 (mRNA) Melon (IVF77) v1

Overview


Name	IVF0001843.1
Type	mRNA
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	GDSL esterase/lipase At5g03980-like
Location	chr05: 23053657 .. 23055095 (-)
Sequence length	1065
RNA-Seq Expression	IVF0001843.1
Synteny	IVF0001843.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCGACGCTATATACCAACTCGGCGACTCAATCTCCGACACCGGAAATCTCATTCGTGAAAACCCCAACACTCCTTTTCTCATCTTCCCTATGGTCAATCTTTCTTCAACAATCCCACCGGTCGATGCTCCAACGGTCTACTTATGCTTGATTTCTTTGGTAAGCATGACCTTCCATGTATATTCTATACATGTTTTTAGCTACTGTTTTCATATGTCGCGAACTAATTATAATAACGATGACTCGATGTATTTGTAGCTTTAGATGCTGGACTTCCTTTGGTGAGTCCTTACTTGAATAAAGATGGTTCGATGGATCATGGAGTCAACTTCGCAGTGGCTGGTTCGACAGCTTTACCTTCTCAATATCTTTTTACAAATTACAAAATTTTATCTCCAGTTACCAACTCTTCTCTCGACCATCAACTTGATTGGATGTTCTCTCATTTCAACTCTATTTGTCACAATCAAAGAGGTGACTGATTAATTAACATACTAGCTACTTACATTTACATATACTAAGATTAATTTGTGTTTCGATGATGTAATGTTTTGATGTTTTCATCTATATTTATGCGTAGATTGCAACGAGAAATTAAGAAGTGCTTTGTTCTTGGTGGGTGAGATTGGTGGCAATGACTATAACTATGCATTATTCCAAGGCAAAACTATTGAAGAGGCCAAAGACATGGTACCTGATGTTGTTCAAACCATTAAGAGTGCCGTTGAGGTATATATATGTTCATTTTGTCTAACAATATAACATAGATGATATATATCCCATATATGAATGCATGAGATGATCACTGTCTTTCTTTTGCTTGTACCTTTGTAGAAAGTAATTAGCTACGGTGCTACTCGAGTTGTTGTTCCTGGAAACTTTCCAATTGGATGCTTCCCTATCTATCTTACTGGATTCCATACTAACGATACATCCGCTTACGACGAGCTTCACTGTTTAAAGGATTTGAATGGTTTGGCAACCTATCATAACAATCAAATCAAGCAAACTATTGAAGTACTCAAGAAAGAGAATCCACATACCGTTATCGTATATGGTGATTATTACAATGCATTTCTATGGATTATTCGTCATGCTTTCGTTCTAGGTACGTAATTTTTTCTCTCCTAGCTACTAACTCTCGATTAAAACATTTCTCAATCTAATTATAATTTTGATCTACTTAATTACTTATATAAATATGTAAAAAACGTACAGGATTTGATGAAGGATCATTGCAAAAATCATGCTGTGGGATTGGAGGAGAGTACAGGTTTAACCTGATGCAAATGTGTGGAGTAGAGGGAGTTGAAGTTTGCCCAAATCCTAATGAACATATCAGTTGGGATGGAGTCCATTTGACACAAAGCACTTACAAGTTCATGACACATTGGCTCATCCATGACATTTTTCCTAAATTGCATTGCATTGTTTAG

mRNA sequence

ATGTTCGACGCTATATACCAACTCGGCGACTCAATCTCCGACACCGGAAATCTCATTCGTGAAAACCCCAACACTCCTTTTCTCATCTTCCCTATGGTCAATCTTTCTTCAACAATCCCACCGGTCGATGCTCCAACGGTCTACTTATGCTTGATTTCTTTGGTAAGCATGACCTTCCATGTATATTCTATACATGTTTTTAGCTACTCTTTAGATGCTGGACTTCCTTTGGTGAGTCCTTACTTGAATAAAGATGGTTCGATGGATCATGGAGTCAACTTCGCAGTGGCTGGTTCGACAGCTTTACCTTCTCAATATCTTTTTACAAATTACAAAATTTTATCTCCAGTTACCAACTCTTCTCTCGACCATCAACTTGATTGGATGTTCTCTCATTTCAACTCTATTTGTCACAATCAAAGAGATTGCAACGAGAAATTAAGAAGTGCTTTGTTCTTGGTGGGTGAGATTGGTGGCAATGACTATAACTATGCATTATTCCAAGGCAAAACTATTGAAGAGGCCAAAGACATGGTACCTGATGTTGTTCAAACCATTAAGAGTGCCGTTGAGAAAGTAATTAGCTACGGTGCTACTCGAGTTGTTGTTCCTGGAAACTTTCCAATTGGATGCTTCCCTATCTATCTTACTGGATTCCATACTAACGATACATCCGCTTACGACGAGCTTCACTGTTTAAAGGATTTGAATGGTTTGGCAACCTATCATAACAATCAAATCAAGCAAACTATTGAAGTACTCAAGAAAGAGAATCCACATACCGTTATCGTATATGGTGATTATTACAATGCATTTCTATGGATTATTCGTCATGCTTTCGTTCTAGGATTTGATGAAGGATCATTGCAAAAATCATGCTGTGGGATTGGAGGAGAGTACAGGTTTAACCTGATGCAAATGTGTGGAGTAGAGGGAGTTGAAGTTTGCCCAAATCCTAATGAACATATCAGTTGGGATGGAGTCCATTTGACACAAAGCACTTACAAGTTCATGACACATTGGCTCATCCATGACATTTTTCCTAAATTGCATTGCATTGTTTAG

Coding sequence (CDS)

ATGTTCGACGCTATATACCAACTCGGCGACTCAATCTCCGACACCGGAAATCTCATTCGTGAAAACCCCAACACTCCTTTTCTCATCTTCCCTATGGTCAATCTTTCTTCAACAATCCCACCGGTCGATGCTCCAACGGTCTACTTATGCTTGATTTCTTTGGTAAGCATGACCTTCCATGTATATTCTATACATGTTTTTAGCTACTCTTTAGATGCTGGACTTCCTTTGGTGAGTCCTTACTTGAATAAAGATGGTTCGATGGATCATGGAGTCAACTTCGCAGTGGCTGGTTCGACAGCTTTACCTTCTCAATATCTTTTTACAAATTACAAAATTTTATCTCCAGTTACCAACTCTTCTCTCGACCATCAACTTGATTGGATGTTCTCTCATTTCAACTCTATTTGTCACAATCAAAGAGATTGCAACGAGAAATTAAGAAGTGCTTTGTTCTTGGTGGGTGAGATTGGTGGCAATGACTATAACTATGCATTATTCCAAGGCAAAACTATTGAAGAGGCCAAAGACATGGTACCTGATGTTGTTCAAACCATTAAGAGTGCCGTTGAGAAAGTAATTAGCTACGGTGCTACTCGAGTTGTTGTTCCTGGAAACTTTCCAATTGGATGCTTCCCTATCTATCTTACTGGATTCCATACTAACGATACATCCGCTTACGACGAGCTTCACTGTTTAAAGGATTTGAATGGTTTGGCAACCTATCATAACAATCAAATCAAGCAAACTATTGAAGTACTCAAGAAAGAGAATCCACATACCGTTATCGTATATGGTGATTATTACAATGCATTTCTATGGATTATTCGTCATGCTTTCGTTCTAGGATTTGATGAAGGATCATTGCAAAAATCATGCTGTGGGATTGGAGGAGAGTACAGGTTTAACCTGATGCAAATGTGTGGAGTAGAGGGAGTTGAAGTTTGCCCAAATCCTAATGAACATATCAGTTGGGATGGAGTCCATTTGACACAAAGCACTTACAAGTTCATGACACATTGGCTCATCCATGACATTTTTCCTAAATTGCATTGCATTGTTTAG

Protein sequence

MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFHVYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNSSLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLATYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEYRFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV

Homology

[Close]

BLAST of IVF0001843.1 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 5.3e-73
Identity = 151/351 (43.02%), Postives = 192/351 (54.70%), Query Frame = 0


Query: 3   DAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFHVY 62
           ++IYQ GDSISDTGNLIR  P                                       
Sbjct: 28  NSIYQFGDSISDTGNLIRNGP--------------------------------------- 87

Query: 63  SIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNSSL 122
                     A  P   P   ++ ++   VNF V+GSTAL S + F+   +  P TN+ L
Sbjct: 88  ----------ASSPTPKPLPQREHNV--FVNFGVSGSTALNSSF-FSERNLHVPATNTPL 147

Query: 123 DHQLDWMFSHFNSICH-NQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVPD 182
             QL W   H  S CH +  DC   L+ +LF+VGEIGGNDYNY  FQGK +EE +  +P 
Sbjct: 148 SMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNYGFFQGKPMEEIRSYIPH 207

Query: 183 VVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLAT 242
           VV  I +A  +VI  GA  VVVPGNFP+GCFPIYLT F   DT  YD+  CL  LN  A 
Sbjct: 208 VVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDYDDNGCLTHLNEFAM 267

Query: 243 YHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEYR 302
            HNNQ+++ I  L+KE P   IVYGDYYNAF +++R      FD+    KSCCG GG Y 
Sbjct: 268 DHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKSVALKSCCGTGGAYN 320

Query: 303 FNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHC 353
           ++  +  G  GV VC NP++ ISWDGVHLTQ  Y+FM+ +L + I  ++ C
Sbjct: 328 YDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQILSQIKC 320

[Close]

BLAST of IVF0001843.1 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 7.1e-70
Identity = 143/351 (40.74%), Postives = 202/351 (57.55%), Query Frame = 0


Query: 2   FDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFHV 61
           FD+IYQLGDS SDTGNLIR  P+ P           T P    PT   C    + + F  
Sbjct: 27  FDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFP--GTPT-GRCSDGRLIIDFIA 86

Query: 62  YSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNSS 121
            +++         LPL++PYL ++ S  HGVNFAVAG+TAL   +L      +S + +S 
Sbjct: 87  TALN---------LPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDI-HSH 146

Query: 122 LDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVPD 181
           L  QL+W  ++  SIC   ++C+ KL++ALF++G IG ND NYA F  +TIEE +  VP 
Sbjct: 147 LSAQLNWFRTYLGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNRTIEEIRAYVPF 206

Query: 182 VVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLAT 241
           + + + +A  ++I  G +RV+VPG FPIGC    L   +       D+L CL  LN L+ 
Sbjct: 207 ITEAVANATREIIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDLGCLSSLNNLSI 266

Query: 242 YHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEYR 301
           Y N+  ++ +  L  E P  VI+Y DYYNA+ ++ R+   LG +  SL K CCGIGG Y 
Sbjct: 267 YFNSLFQRALASLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLLKCCCGIGGPYN 326

Query: 302 FNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHC 353
           ++  + CG  GV VCPNP ++I WDG H TQ+ Y+ +  ++I  I   L C
Sbjct: 327 YDPDRECGSRGVPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKALKC 363

[Close]

BLAST of IVF0001843.1 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 236.9 bits (603), Expect = 3.5e-61
Identity = 131/346 (37.86%), Postives = 197/346 (56.94%), Query Frame = 0


Query: 2   FDAIYQLGDSISDTGNLI-RENPN-TPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTF 61
           F +I   GDSI+DTGNL+   +PN  P   FP    +    P    +    +I  ++   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 62  HVYSIHVFSYSLDAGLPLVSPYLN-KDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVT 121
                         G PLV P+   ++ +   GVNFAVAG+TAL   +L     I S +T
Sbjct: 94  --------------GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFL-EERGIHSTIT 153

Query: 122 NSSLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDM 181
           N SL  QL        ++C +  DC + + +AL L+GEIGGNDYN+ALFQ K ++E +++
Sbjct: 154 NVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEEL 213

Query: 182 VPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDEL-HCLKDLN 241
           VP V+ TI SA+ +++  G    +VPGNFPIG    YLT + T++   YD L  CLK LN
Sbjct: 214 VPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLN 273

Query: 242 GLATYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIG 301
             + Y+N Q+++ +  L+K  PH  I+Y DYYNA L + +     GF    L  +CCG+G
Sbjct: 274 DFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVG 333

Query: 302 GEYRFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLI 344
           G Y FN  + CG  GVE C +P++++++DG+H+T++ Y+ ++  L+
Sbjct: 334 GSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

[Close]

BLAST of IVF0001843.1 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.6e-61
Identity = 128/347 (36.89%), Postives = 191/347 (55.04%), Query Frame = 0


Query: 2   FDAIYQLGDSISDTGNLI--RENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTF 61
           F +I   GDSI+DTGNL+   +    P   FP    +    P         ++  ++   
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVTAFPPYGETFFHHPTGRSCDGRIIMDFIAEF- 88

Query: 62  HVYSIHVFSYSLDAGLPLVSPYL-NKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVT 121
                         GLP V PY  +K+ + D GVNFAVAG+TAL S +L    + + P T
Sbjct: 89  -------------VGLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFL--KKRGIQPHT 148

Query: 122 NSSLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDM 181
           N SL  QL        ++C +  DC + + +AL L+GEIGGNDYN+  F  K ++E +++
Sbjct: 149 NVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEEL 208

Query: 182 VPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYD-ELHCLKDLN 241
           VP V+ +I S + ++I  G    +VPG FPIGC  +YLT + T++   YD    CLK LN
Sbjct: 209 VPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLN 268

Query: 242 GLATYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIG 301
               YH+ ++K  +  L+K  PH  I+Y DYYN+ L I +     GF E     +CCGIG
Sbjct: 269 KFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPF-PACCGIG 328

Query: 302 GEYRFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIH 345
           G Y FN  + CG  GV+ C +P++++ WDGVH+T++ YK++   +++
Sbjct: 329 GPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIADGILN 358

[Close]

BLAST of IVF0001843.1 vs. ExPASy Swiss-Prot
Match: Q9C857 (GDSL esterase/lipase At1g31550 OS=Arabidopsis thaliana OX=3702 GN=At1g31550 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.9e-60
Identity = 131/346 (37.86%), Postives = 192/346 (55.49%), Query Frame = 0


Query: 2   FDAIYQLGDSISDTGNL--IRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTF 61
           F++I   GDSI+DTGNL  + ++ N P   FP    +    P    +    +I  ++   
Sbjct: 34  FESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGETFFHHPTGRFSDGRLIIDFIAEFL 93

Query: 62  HVYSIHVFSYSLDAGLPLVSPYL-NKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVT 121
                         GLP V PY  + +G+ + GVNFAVA +TAL S +L    K      
Sbjct: 94  --------------GLPYVPPYFGSTNGNFEKGVNFAVASATALESSFL--EEKGYHCPH 153

Query: 122 NSSLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDM 181
           N SL  QL        ++C    DC + + +AL L+GEIG NDYN+  FQ + ++E K++
Sbjct: 154 NFSLGVQLKIFKQSLPNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQLRPLDEVKEL 213

Query: 182 VPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDEL-HCLKDLN 241
           VP V+ TI SA+ ++I  G    +VPG FP+GC   +LT   T++   YD L  CLK LN
Sbjct: 214 VPLVISTISSAITELIGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDPLTGCLKWLN 273

Query: 242 GLATYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIG 301
               YH+ Q+++ +  L+K NPH  I+Y DYYNA L + R     GF    L  +CCG+G
Sbjct: 274 KFGEYHSEQLQEELNRLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRHL-SACCGVG 333

Query: 302 GEYRFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLI 344
           G Y FNL + CG  GVE C +P+++++WDG+H+T++ +K M   L+
Sbjct: 334 GPYNFNLSRSCGSVGVEACSDPSKYVAWDGLHMTEAAHKSMADGLV 362

[Close]

BLAST of IVF0001843.1 vs. ExPASy TrEMBL
Match: A0A5A7TWR0 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001010 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.2e-182
Identity = 315/354 (88.98%), Postives = 320/354 (90.40%), Query Frame = 0


Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFH 60
           MFDAIYQLGDSISDTGNLIRENPNTPF   P        P         C   L+ + F 
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNP------TGRCSNGLLMLDF- 60

Query: 61  VYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120
                   ++LDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS
Sbjct: 61  --------FALDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120

Query: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180
           SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP
Sbjct: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180

Query: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240
           DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA
Sbjct: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240

Query: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300
           TY+NNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY
Sbjct: 241 TYYNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300

Query: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 355
           RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV
Sbjct: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 339

[Close]

BLAST of IVF0001843.1 vs. ExPASy TrEMBL
Match: A0A5A7VJA5 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00480 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 6.1e-181
Identity = 314/354 (88.70%), Postives = 318/354 (89.83%), Query Frame = 0


Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFH 60
           MFDAIYQLGDSISDTGNLIRENPNTPF   P        P         C   L+ + F 
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNP------TGRCSNGLLMLDF- 60

Query: 61  VYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120
                   ++LDAGL LVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS
Sbjct: 61  --------FALDAGLSLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120

Query: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180
           SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP
Sbjct: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180

Query: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240
           DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA
Sbjct: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240

Query: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300
           TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDE SLQKSCCGIGGEY
Sbjct: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGEY 300

Query: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 355
           RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV
Sbjct: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 339

[Close]

BLAST of IVF0001843.1 vs. ExPASy TrEMBL
Match: A0A1S4E629 (acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503259 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 3.6e-173
Identity = 300/354 (84.75%), Postives = 308/354 (87.01%), Query Frame = 0


Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFH 60
           MFDAIYQLGDSISDTGNLIRENPNTPF   P        P         C   L+ + F 
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNP------TGRCSNGLLMLDF- 91

Query: 61  VYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120
                   ++LDAGLPLVSPYLNKDG MDHGVNFAVAGSTAL SQYL T YKILSPVTNS
Sbjct: 92  --------FALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNS 151

Query: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180
           SLDHQL+WMFSHFNSICHNQRDCNEKLRSALF VGEIGGNDYNYALFQGKTI+EAKDMVP
Sbjct: 152 SLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVP 211

Query: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240
           DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNG A
Sbjct: 212 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFA 271

Query: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300
           TYHN+QIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHA VLGFDE SLQKSCCGIGG+Y
Sbjct: 272 TYHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDY 331

Query: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 355
            FNLMQMCGV GVE CPNPNEHISWDGVHLTQ TYKFMTHWLIHDIFPKLHCIV
Sbjct: 332 NFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

[Close]

BLAST of IVF0001843.1 vs. ExPASy TrEMBL
Match: A0A5D3CNR6 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold745G00130 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.4e-172
Identity = 299/354 (84.46%), Postives = 308/354 (87.01%), Query Frame = 0


Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFH 60
           MFDAIYQLGDSISDTGNLIRENPNTPF   P        P         C   L+ + F 
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNP------TGRCSNGLLMLDF- 91

Query: 61  VYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120
                   ++LDAGLPLVSPYLNKDG MDHGVNFAVAGSTAL SQYL T YKILSPVTNS
Sbjct: 92  --------FALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNS 151

Query: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180
           SLDHQL+WMFSHFNSICHNQRDCNEKLRSALF VGEIGGNDYNYALFQGKTI+EAKDMVP
Sbjct: 152 SLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVP 211

Query: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240
           DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNG A
Sbjct: 212 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFA 271

Query: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300
           T+HN+QIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHA VLGFDE SLQKSCCGIGG+Y
Sbjct: 272 THHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDY 331

Query: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 355
            FNLMQMCGV GVE CPNPNEHISWDGVHLTQ TYKFMTHWLIHDIFPKLHCIV
Sbjct: 332 NFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

[Close]

BLAST of IVF0001843.1 vs. ExPASy TrEMBL
Match: A0A5A7SSW2 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold219G001390 PE=3 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 3.0e-172
Identity = 299/354 (84.46%), Postives = 307/354 (86.72%), Query Frame = 0


Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFH 60
           MFDAIYQLGDSISDTGNLIRENPNTPF   P        P         C   L+ + F 
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNP------TGRCSNGLLMLDF- 91

Query: 61  VYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120
                   ++LDAGLPLVSPYLNKDG MDHGVNFAVAGSTAL SQYL T YKILSPVTNS
Sbjct: 92  --------FALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNS 151

Query: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180
           SLDHQL+WMFSHFNSICHNQRDCNEKLRSALF VGEIGGNDYNYALFQGKTI+EAKDMVP
Sbjct: 152 SLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVP 211

Query: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240
           DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNG A
Sbjct: 212 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFA 271

Query: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300
           TYHN+QIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHA VLGFDE SLQKSCCGIGG+Y
Sbjct: 272 TYHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDY 331

Query: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 355
            FNLMQMCGV GVE CPNPNEHISWD VHLTQ TYKFMTHWLIHDIFPKLHCIV
Sbjct: 332 NFNLMQMCGVAGVEACPNPNEHISWDRVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

[Close]

BLAST of IVF0001843.1 vs. NCBI nr
Match: KAA0048033.1 (acetylajmalan esterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 645 bits (1663), Expect = 1.70e-232
Identity = 315/354 (88.98%), Postives = 320/354 (90.40%), Query Frame = 0


Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFH 60
           MFDAIYQLGDSISDTGNLIRENPNTPF   P        P         C   L+ + F 
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGR------CSNGLLMLDF- 60

Query: 61  VYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120
                   ++LDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS
Sbjct: 61  --------FALDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120

Query: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180
           SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP
Sbjct: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180

Query: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240
           DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA
Sbjct: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240

Query: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300
           TY+NNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY
Sbjct: 241 TYYNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300

Query: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 354
           RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV
Sbjct: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 339

[Close]

BLAST of IVF0001843.1 vs. NCBI nr
Match: KAA0065761.1 (acetylajmalan esterase-like [Cucumis melo var. makuwa] >TYJ96502.1 acetylajmalan esterase-like [Cucumis melo var. makuwa] >TYK13786.1 acetylajmalan esterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 641 bits (1653), Expect = 5.69e-231
Identity = 314/354 (88.70%), Postives = 318/354 (89.83%), Query Frame = 0


Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFH 60
           MFDAIYQLGDSISDTGNLIRENPNTPF   P        P         C   L+ + F 
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGR------CSNGLLMLDF- 60

Query: 61  VYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120
                   ++LDAGL LVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS
Sbjct: 61  --------FALDAGLSLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120

Query: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180
           SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP
Sbjct: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180

Query: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240
           DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA
Sbjct: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240

Query: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300
           TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDE SLQKSCCGIGGEY
Sbjct: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGEY 300

Query: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 354
           RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV
Sbjct: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 339

[Close]

BLAST of IVF0001843.1 vs. NCBI nr
Match: XP_016903430.1 (PREDICTED: acetylajmalan esterase-like [Cucumis melo])

HSP 1 Score: 615 bits (1586), Expect = 2.91e-220
Identity = 300/354 (84.75%), Postives = 308/354 (87.01%), Query Frame = 0


Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFH 60
           MFDAIYQLGDSISDTGNLIRENPNTPF   P        P         C   L+ + F 
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGR------CSNGLLMLDF- 91

Query: 61  VYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120
                   ++LDAGLPLVSPYLNKDG MDHGVNFAVAGSTAL SQYL T YKILSPVTNS
Sbjct: 92  --------FALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNS 151

Query: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180
           SLDHQL+WMFSHFNSICHNQRDCNEKLRSALF VGEIGGNDYNYALFQGKTI+EAKDMVP
Sbjct: 152 SLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVP 211

Query: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240
           DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNG A
Sbjct: 212 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFA 271

Query: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300
           TYHN+QIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHA VLGFDE SLQKSCCGIGG+Y
Sbjct: 272 TYHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDY 331

Query: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 354
            FNLMQMCGV GVE CPNPNEHISWDGVHLTQ TYKFMTHWLIHDIFPKLHCIV
Sbjct: 332 NFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

[Close]

BLAST of IVF0001843.1 vs. NCBI nr
Match: KAE8646130.1 (hypothetical protein Csa_016115 [Cucumis sativus])

HSP 1 Score: 613 bits (1580), Expect = 7.57e-220
Identity = 298/354 (84.18%), Postives = 309/354 (87.29%), Query Frame = 0


Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFH 60
           MFDAIYQLGDSISDTGNLIRENPNTPF   P        P         C   L+ + F 
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGR------CSNGLLMLDF- 60

Query: 61  VYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120
                    +LDAGLPLVSPYLNKDG MDHGVNFAVAGSTALPSQYL ++YKI+SPVTNS
Sbjct: 61  --------LALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSYKIISPVTNS 120

Query: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180
           SLDHQLDWMFSHFNSICHNQR+CNEKLRSALFLVGEIGGNDYNYALFQGKTI+EAKDMVP
Sbjct: 121 SLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKTIQEAKDMVP 180

Query: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240
           DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLN  A
Sbjct: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFA 240

Query: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300
           TYHN+QIKQ IEVLKKENPH VIVYGDYYNAFLWIIRHAFVLGFDE SLQKSCCGIGG+Y
Sbjct: 241 TYHNDQIKQAIEVLKKENPHAVIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDY 300

Query: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 354
           +FNLMQMCGV GVE CPNPNEHISWDGVHLTQ TYKFMTHWLIHDIFPKLHCIV
Sbjct: 301 KFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 339

[Close]

BLAST of IVF0001843.1 vs. NCBI nr
Match: TYK13517.1 (acetylajmalan esterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 613 bits (1581), Expect = 1.68e-219
Identity = 299/354 (84.46%), Postives = 308/354 (87.01%), Query Frame = 0


Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFH 60
           MFDAIYQLGDSISDTGNLIRENPNTPF   P        P         C   L+ + F 
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGR------CSNGLLMLDF- 91

Query: 61  VYSIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNS 120
                   ++LDAGLPLVSPYLNKDG MDHGVNFAVAGSTAL SQYL T YKILSPVTNS
Sbjct: 92  --------FALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNS 151

Query: 121 SLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVP 180
           SLDHQL+WMFSHFNSICHNQRDCNEKLRSALF VGEIGGNDYNYALFQGKTI+EAKDMVP
Sbjct: 152 SLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVP 211

Query: 181 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLA 240
           DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNG A
Sbjct: 212 DVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFA 271

Query: 241 TYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEY 300
           T+HN+QIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHA VLGFDE SLQKSCCGIGG+Y
Sbjct: 272 THHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDY 331

Query: 301 RFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 354
            FNLMQMCGV GVE CPNPNEHISWDGVHLTQ TYKFMTHWLIHDIFPKLHCIV
Sbjct: 332 NFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

[Close]

BLAST of IVF0001843.1 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.7e-74
Identity = 151/351 (43.02%), Postives = 192/351 (54.70%), Query Frame = 0


Query: 3   DAIYQLGDSISDTGNLIRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTFHVY 62
           ++IYQ GDSISDTGNLIR  P                                       
Sbjct: 28  NSIYQFGDSISDTGNLIRNGP--------------------------------------- 87

Query: 63  SIHVFSYSLDAGLPLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNSSL 122
                     A  P   P   ++ ++   VNF V+GSTAL S + F+   +  P TN+ L
Sbjct: 88  ----------ASSPTPKPLPQREHNV--FVNFGVSGSTALNSSF-FSERNLHVPATNTPL 147

Query: 123 DHQLDWMFSHFNSICH-NQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVPD 182
             QL W   H  S CH +  DC   L+ +LF+VGEIGGNDYNY  FQGK +EE +  +P 
Sbjct: 148 SMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNYGFFQGKPMEEIRSYIPH 207

Query: 183 VVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLAT 242
           VV  I +A  +VI  GA  VVVPGNFP+GCFPIYLT F   DT  YD+  CL  LN  A 
Sbjct: 208 VVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDYDDNGCLTHLNEFAM 267

Query: 243 YHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEYR 302
            HNNQ+++ I  L+KE P   IVYGDYYNAF +++R      FD+    KSCCG GG Y 
Sbjct: 268 DHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKSVALKSCCGTGGAYN 320

Query: 303 FNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHC 353
           ++  +  G  GV VC NP++ ISWDGVHLTQ  Y+FM+ +L + I  ++ C
Sbjct: 328 YDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQILSQIKC 320

[Close]

BLAST of IVF0001843.1 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 236.9 bits (603), Expect = 2.5e-62
Identity = 131/346 (37.86%), Postives = 197/346 (56.94%), Query Frame = 0


Query: 2   FDAIYQLGDSISDTGNLI-RENPN-TPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTF 61
           F +I   GDSI+DTGNL+   +PN  P   FP    +    P    +    +I  ++   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 62  HVYSIHVFSYSLDAGLPLVSPYLN-KDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVT 121
                         G PLV P+   ++ +   GVNFAVAG+TAL   +L     I S +T
Sbjct: 94  --------------GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFL-EERGIHSTIT 153

Query: 122 NSSLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDM 181
           N SL  QL        ++C +  DC + + +AL L+GEIGGNDYN+ALFQ K ++E +++
Sbjct: 154 NVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEEL 213

Query: 182 VPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDEL-HCLKDLN 241
           VP V+ TI SA+ +++  G    +VPGNFPIG    YLT + T++   YD L  CLK LN
Sbjct: 214 VPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLN 273

Query: 242 GLATYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIG 301
             + Y+N Q+++ +  L+K  PH  I+Y DYYNA L + +     GF    L  +CCG+G
Sbjct: 274 DFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVG 333

Query: 302 GEYRFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLI 344
           G Y FN  + CG  GVE C +P++++++DG+H+T++ Y+ ++  L+
Sbjct: 334 GSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

[Close]

BLAST of IVF0001843.1 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 236.5 bits (602), Expect = 3.3e-62
Identity = 128/347 (36.89%), Postives = 191/347 (55.04%), Query Frame = 0


Query: 2   FDAIYQLGDSISDTGNLI--RENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTF 61
           F +I   GDSI+DTGNL+   +    P   FP    +    P         ++  ++   
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVTAFPPYGETFFHHPTGRSCDGRIIMDFIAEF- 88

Query: 62  HVYSIHVFSYSLDAGLPLVSPYL-NKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVT 121
                         GLP V PY  +K+ + D GVNFAVAG+TAL S +L    + + P T
Sbjct: 89  -------------VGLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFL--KKRGIQPHT 148

Query: 122 NSSLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDM 181
           N SL  QL        ++C +  DC + + +AL L+GEIGGNDYN+  F  K ++E +++
Sbjct: 149 NVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEEL 208

Query: 182 VPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYD-ELHCLKDLN 241
           VP V+ +I S + ++I  G    +VPG FPIGC  +YLT + T++   YD    CLK LN
Sbjct: 209 VPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLN 268

Query: 242 GLATYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIG 301
               YH+ ++K  +  L+K  PH  I+Y DYYN+ L I +     GF E     +CCGIG
Sbjct: 269 KFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPF-PACCGIG 328

Query: 302 GEYRFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIH 345
           G Y FN  + CG  GV+ C +P++++ WDGVH+T++ YK++   +++
Sbjct: 329 GPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIADGILN 358

[Close]

BLAST of IVF0001843.1 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.8e-61
Identity = 130/347 (37.46%), Postives = 191/347 (55.04%), Query Frame = 0


Query: 2   FDAIYQLGDSISDTGNL--IRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTF 61
           F +I   GDSI+DTGNL  + +  + P + FP    +    P    +    +I  ++   
Sbjct: 35  FKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENFFHHPTGRFSNGRLIIDFIAEFL 94

Query: 62  HVYSIHVFSYSLDAGLPLVSP-YLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVT 121
                         GLPLV P Y + + + + GVNFAV G+TAL   +L  +  I  P T
Sbjct: 95  --------------GLPLVPPFYGSHNANFEKGVNFAVGGATALERSFL-EDRGIHFPYT 154

Query: 122 NSSLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDM 181
           N SL  QL+       SIC +  DC + + +AL L+GEIGGNDYNYA F  K IEE K++
Sbjct: 155 NVSLGVQLNSFKESLPSICGSPSDCRDMIENALILMGEIGGNDYNYAFFVDKGIEEIKEL 214

Query: 182 VPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDEL-HCLKDLN 241
           +P V+ TI SA+ ++I  G    +VPG FP+GC  +YLT   T++   YD L  CLK LN
Sbjct: 215 MPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSHQTSNMEEYDPLTGCLKWLN 274

Query: 242 GLATYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIG 301
                H  Q++  +  L+K  PH  I+Y DYYNA   + +     GF    L  +CCG G
Sbjct: 275 KFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQEPAKFGFMNRPL-SACCGAG 334

Query: 302 GEYRFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLIH 345
           G Y + + + CG + VE C +P+++++WDGVH+T++ Y+ M   +++
Sbjct: 335 GPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLMAEGILN 365

[Close]

BLAST of IVF0001843.1 vs. TAIR 10
Match: AT1G31550.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.8e-61
Identity = 131/346 (37.86%), Postives = 192/346 (55.49%), Query Frame = 0


Query: 2   FDAIYQLGDSISDTGNL--IRENPNTPFLIFPMVNLSSTIPPVDAPTVYLCLISLVSMTF 61
           F++I   GDSI+DTGNL  + ++ N P   FP    +    P    +    +I  ++   
Sbjct: 34  FESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGETFFHHPTGRFSDGRLIIDFIAEFL 93

Query: 62  HVYSIHVFSYSLDAGLPLVSPYL-NKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVT 121
                         GLP V PY  + +G+ + GVNFAVA +TAL S +L    K      
Sbjct: 94  --------------GLPYVPPYFGSTNGNFEKGVNFAVASATALESSFL--EEKGYHCPH 153

Query: 122 NSSLDHQLDWMFSHFNSICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDM 181
           N SL  QL        ++C    DC + + +AL L+GEIG NDYN+  FQ + ++E K++
Sbjct: 154 NFSLGVQLKIFKQSLPNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQLRPLDEVKEL 213

Query: 182 VPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDEL-HCLKDLN 241
           VP V+ TI SA+ ++I  G    +VPG FP+GC   +LT   T++   YD L  CLK LN
Sbjct: 214 VPLVISTISSAITELIGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDPLTGCLKWLN 273

Query: 242 GLATYHNNQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIG 301
               YH+ Q+++ +  L+K NPH  I+Y DYYNA L + R     GF    L  +CCG+G
Sbjct: 274 KFGEYHSEQLQEELNRLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRHL-SACCGVG 333

Query: 302 GEYRFNLMQMCGVEGVEVCPNPNEHISWDGVHLTQSTYKFMTHWLI 344
           G Y FNL + CG  GVE C +P+++++WDG+H+T++ +K M   L+
Sbjct: 334 GPYNFNLSRSCGSVGVEACSDPSKYVAWDGLHMTEAAHKSMADGLV 362

The following BLAST results are available for this feature:

BLAST of IVF0001843.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZB2	5.3e-73	43.02	GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2...	[more]
Q3MKY2	7.1e-70	40.74	Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1	[more]
Q8RXT9	3.5e-61	37.86	GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2...	[more]
Q94F40	4.6e-61	36.89	GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2...	[more]
Q9C857	3.9e-60	37.86	GDSL esterase/lipase At1g31550 OS=Arabidopsis thaliana OX=3702 GN=At1g31550 PE=2...	[more]

BLAST of IVF0001843.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TWR0	4.2e-182	88.98	Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A5A7VJA5	6.1e-181	88.70	Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S4E629	3.6e-173	84.75	acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503259 PE=3 SV=1	[more]
A0A5D3CNR6	1.4e-172	84.46	Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A5A7SSW2	3.0e-172	84.46	Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]

BLAST of IVF0001843.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0048033.1	1.70e-232	88.98	acetylajmalan esterase-like [Cucumis melo var. makuwa]	[more]
KAA0065761.1	5.69e-231	88.70	acetylajmalan esterase-like [Cucumis melo var. makuwa] >TYJ96502.1 acetylajmalan...	[more]
XP_016903430.1	2.91e-220	84.75	PREDICTED: acetylajmalan esterase-like [Cucumis melo]	[more]
KAE8646130.1	7.57e-220	84.18	hypothetical protein Csa_016115 [Cucumis sativus]	[more]
TYK13517.1	1.68e-219	84.46	acetylajmalan esterase-like [Cucumis melo var. makuwa]	[more]

BLAST of IVF0001843.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03980.1	3.7e-74	43.02	SGNH hydrolase-type esterase superfamily protein	[more]
AT1G28590.1	2.5e-62	37.86	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G28600.1	3.3e-62	36.89	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G28580.1	2.8e-61	37.46	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G31550.2	2.8e-61	37.86	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 5..342 e-value: 4.7E-23 score: 82.8
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 1..346 e-value: 1.2E-56 score: 194.5
None	No IPR available	PANTHER	PTHR22835	ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN	coord: 2..31
None	No IPR available	PANTHER	PTHR22835:SF581	ACETYLAJMALAN ESTERASE-LIKE	coord: 2..31 coord: 70..352
None	No IPR available	PANTHER	PTHR22835	ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN	coord: 70..352
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 5..344

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
IVF0001843	IVF0001843	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
IVF0001843.1-exon	IVF0001843.1-exon-chr05:23053657..23053874	exon
IVF0001843.1-exon	IVF0001843.1-exon-chr05:23053985..23054258	exon
IVF0001843.1-exon	IVF0001843.1-exon-chr05:23054364..23054512	exon
IVF0001843.1-exon	IVF0001843.1-exon-chr05:23054619..23054834	exon
IVF0001843.1-exon	IVF0001843.1-exon-chr05:23054888..23055095	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
IVF0001843.1-cds	IVF0001843.1-cds-chr05:23053657..23053874	CDS
IVF0001843.1-cds	IVF0001843.1-cds-chr05:23053985..23054258	CDS
IVF0001843.1-cds	IVF0001843.1-cds-chr05:23054364..23054512	CDS
IVF0001843.1-cds	IVF0001843.1-cds-chr05:23054619..23054834	CDS
IVF0001843.1-cds	IVF0001843.1-cds-chr05:23054888..23055095	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
IVF0001843.1	IVF0001843.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds