HG10023306.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023306.1 vs. NCBI nr
Match: XP_038896850.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Benincasa hispida])

HSP 1 Score: 612.5 bits (1578), Expect = 2.1e-171
Identity = 294/313 (93.93%), Postives = 299/313 (95.53%), Query Frame = 0

Query: 1   MGSETEGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEVLK 60
           MGSET GLPVINFSG  VK EG  EWRR SR+VREALEEYGCFIAEY+GVS+ELRGEV+K
Sbjct: 1   MGSETGGLPVINFSGNGVKTEGGDEWRRRSREVREALEEYGCFIAEYDGVSRELRGEVMK 60

Query: 61  AVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMWPSG 120
           AVKALFELPIE KKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGV SFTHLMWPSG
Sbjct: 61  AVKALFELPIERKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVQSFTHLMWPSG 120

Query: 121 NHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQSPNP 180
           NHHFS TLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAP S NP
Sbjct: 121 NHHFSTTLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPHSSNP 180

Query: 181 TLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSNDRI 240
           TLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVD SSPS+FVVIAGDAFKAWSNDRI
Sbjct: 181 TLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDNSSPSTFVVIAGDAFKAWSNDRI 240

Query: 241 ESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEG 300
           ESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQ HPLRYKPFDHIGLLHFFRSKEG
Sbjct: 241 ESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQTHPLRYKPFDHIGLLHFFRSKEG 300

Query: 301 FNSKSPLKDYCAL 314
           FNSKSPLKDYCAL
Sbjct: 301 FNSKSPLKDYCAL 313

BLAST of HG10023306.1 vs. NCBI nr
Match: XP_004136074.1 (2-oxoglutarate-dependent dioxygenase AOP2 isoform X1 [Cucumis sativus] >KAE8646455.1 hypothetical protein Csa_015846 [Cucumis sativus])

HSP 1 Score: 577.8 bits (1488), Expect = 5.7e-161
Identity = 282/317 (88.96%), Postives = 296/317 (93.38%), Query Frame = 0

Query: 1   MGSETE---GLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGE 60
           MGSET     LPVINFS EKVKAEG  EWRRTS+ VREA+EEYGCFIA+YEGVSKELR E
Sbjct: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60

Query: 61  VLKAVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMW 120
           VLKAVK LFELPIE KK NKYEKPFNGYVGELRTLPLHESLGIDDATNF+ V SF+HLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120

Query: 121 -PSGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQ 180
            P GNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKY+ SHADSITYLLR+LK+KAPQ
Sbjct: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180

Query: 181 SPNPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWS 240
           SP+PTLCLVDHTDKSFTTM+YQDHINGLE+KTK+GQWIQVD SSPSSF+VIAGDA KAWS
Sbjct: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS 240

Query: 241 NDRIESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 300
           NDRIESPTHKV IRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR
Sbjct: 241 NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 300

Query: 301 SKEGFNSKSPLKDYCAL 314
           SKEGFNSKSPLKDYCA+
Sbjct: 301 SKEGFNSKSPLKDYCAI 317

BLAST of HG10023306.1 vs. NCBI nr
Match: XP_023548444.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562.8 bits (1449), Expect = 1.9e-156
Identity = 270/318 (84.91%), Postives = 289/318 (90.88%), Query Frame = 0

Query: 1   MGSETE-----GLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELR 60
           MGSET+     GLPVINFSG+ VKAEG   WRR S +VREALEEYGCFIAEYEG+S ELR
Sbjct: 1   MGSETDPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  GEVLKAVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHL 120
            EV KAV+ALF+LPIETKKKN+YEKPFNGYVGELRTLPLHESLGIDDATN HGV +FTHL
Sbjct: 61  VEVFKAVEALFDLPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIHGVQAFTHL 120

Query: 121 MWPSGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAP 180
           MWP+GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKYH++HA SITYLLR LKNK P
Sbjct: 121 MWPNGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKTP 180

Query: 181 QSPNPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAW 240
            S NPTLCLVDHTDKSFTTM+YQDH NGLEV+TK G+WIQVD SSPS+FVVIAGDAFKAW
Sbjct: 181 HSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKAW 240

Query: 241 SNDRIESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SNDRIESPTHKV IRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLHFF
Sbjct: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFF 300

Query: 301 RSKEGFNSKSPLKDYCAL 314
           RSKEGF SKSPL+DYCAL
Sbjct: 301 RSKEGFKSKSPLQDYCAL 318

BLAST of HG10023306.1 vs. NCBI nr
Match: XP_022953974.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita moschata])

HSP 1 Score: 560.8 bits (1444), Expect = 7.3e-156
Identity = 271/318 (85.22%), Postives = 288/318 (90.57%), Query Frame = 0

Query: 1   MGSET-----EGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELR 60
           MGSET      GLPVINFSG+ VKAEG   WRR S +VREALEEYGCFIAEYEG+S ELR
Sbjct: 1   MGSETGPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  GEVLKAVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHL 120
            EV KAV+ALFELPIETKKKN+YEKPFNGYVGELRTLPLHESLGIDDATN  GV +FTHL
Sbjct: 61  VEVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTHL 120

Query: 121 MWPSGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAP 180
           MWP+GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKYH++HA SITYLLR LKNKAP
Sbjct: 121 MWPNGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKAP 180

Query: 181 QSPNPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAW 240
            S NPTLCLVDHTDKSFTTM+YQDH NGLEV+TK G+WIQVD SSPS+FVVIAGDAFKAW
Sbjct: 181 HSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKAW 240

Query: 241 SNDRIESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SNDRIESPTHKV IRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLHFF
Sbjct: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFF 300

Query: 301 RSKEGFNSKSPLKDYCAL 314
           RSKEGF SKSPL+DYCAL
Sbjct: 301 RSKEGFKSKSPLQDYCAL 318

BLAST of HG10023306.1 vs. NCBI nr
Match: XP_022991572.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita maxima])

HSP 1 Score: 557.0 bits (1434), Expect = 1.0e-154
Identity = 269/318 (84.59%), Postives = 288/318 (90.57%), Query Frame = 0

Query: 1   MGSET-----EGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELR 60
           MGSET      GLPVINFSG+ VKAEG   WRR S +VREALEEYGCFIAEYEG+S ELR
Sbjct: 1   MGSETGPRMDAGLPVINFSGDDVKAEGGEVWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  GEVLKAVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHL 120
            +V KAV+ALFELPIETKKKN+YEKPFNGYVGELRTLPLHESLGIDDATN  GV +FT+L
Sbjct: 61  VQVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTNL 120

Query: 121 MWPSGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAP 180
           MWP+GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKYH++HA SITYLLR LKNKAP
Sbjct: 121 MWPNGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKAP 180

Query: 181 QSPNPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAW 240
            S NPTLCLVDHTDKSFTTM+YQDH NGLEV+TK G+WIQVD SSPS+FVVIAGDAFKAW
Sbjct: 181 HSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKAW 240

Query: 241 SNDRIESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SNDRIESPTHKV IRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLHFF
Sbjct: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFF 300

Query: 301 RSKEGFNSKSPLKDYCAL 314
           RSKEGF SKSPL+DYCAL
Sbjct: 301 RSKEGFKSKSPLQDYCAL 318

BLAST of HG10023306.1 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.7e-62
Identity = 132/313 (42.17%), Postives = 191/313 (61.02%), Query Frame = 0

Query: 8   LPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEVLKAVKALFE 67
           LPVI+FS + +K  GS +W      V +ALE+YGCF A ++ +S EL   V +A++ LFE
Sbjct: 14  LPVIDFSDQNLK-PGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFE 73

Query: 68  LPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMWPS-GNHHFSA 127
           LPI TK++N   KPF+GY+       L+ESLGIDDA     V+ FT  +WP  GN   S 
Sbjct: 74  LPIPTKQRNVSSKPFHGYLCH----NLYESLGIDDANVLEKVNDFTQQLWPDHGNKSISE 133

Query: 128 TLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAP-----QSPNPT 187
           T+H F++   ELD MV +MI ES+G+E Y   H +S  YL R++K  +P           
Sbjct: 134 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEETK 193

Query: 188 LCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSNDRIE 247
           L L  HTDK+  T+L+Q  ++GLEVKTK  +WI+V   S  S +V+ GD+  A  N R+ 
Sbjct: 194 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVK-PSQDSVLVMVGDSLCALLNGRLH 253

Query: 248 SPTHKVMIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKE 307
           SP H+V++ G + RYS  +F    +  I++ P+ELVD+ HP  +KPF++   LHFF+++ 
Sbjct: 254 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 313

Query: 308 GFNSKSPLKDYCA 313
           G  ++S L  + A
Sbjct: 314 GRIAQSALHAFAA 320

BLAST of HG10023306.1 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.4e-62
Identity = 131/314 (41.72%), Postives = 192/314 (61.15%), Query Frame = 0

Query: 8   LPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEVLKAVKALFE 67
           LPVI+FS + +K  GS +W   +  V +ALE+YGCF A ++ +S EL   V +A++ LFE
Sbjct: 14  LPVIDFSDQNLK-PGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 73

Query: 68  LPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMWPS-GNHHFSA 127
           LPI TK++N   KPF+GY+       L+ESLGI+DA     V+ FT  +WP  GN   S 
Sbjct: 74  LPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 133

Query: 128 TLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAP------QSPNP 187
           T+H F++   ELD MV +MI ES+G+E Y   H +S  YL R++K  +P           
Sbjct: 134 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEET 193

Query: 188 TLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSNDRI 247
            L L  HTDK+  T+L+Q  ++GLEVKTK  +WI+V   S  S +V+ GD+  A  N R+
Sbjct: 194 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVK-PSQDSVLVMVGDSLCALLNGRL 253

Query: 248 ESPTHKVMIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSK 307
            SP H+V++ G + RYS  +F    +  I++ P+ELVD+ HP  +KPF++   LHFF+++
Sbjct: 254 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 313

Query: 308 EGFNSKSPLKDYCA 313
            G  ++S L  + A
Sbjct: 314 AGRIAQSALHAFAA 321

BLAST of HG10023306.1 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.6e-60
Identity = 128/300 (42.67%), Postives = 186/300 (62.00%), Query Frame = 0

Query: 8   LPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEVLKAVKALFE 67
           LPVI+FS + +K  GS +W   +  V +ALE+YGCF A ++ +S EL   V +A++ LFE
Sbjct: 7   LPVIDFSDQNLK-PGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 66

Query: 68  LPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMWPS-GNHHFSA 127
           LPI TK++N   K F+GY+       L+ESLGI+DA     V+ FT  +WP  GN   S 
Sbjct: 67  LPIPTKQRNVSSKLFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 126

Query: 128 TLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAP----QSPNPTL 187
           T+H F++   ELD MV +MI ES+G+EKY   H +S  YL R++K  +P          L
Sbjct: 127 TIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETKL 186

Query: 188 CLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSNDRIES 247
            L  HTDK+  T+L+Q  ++GLEVKTK  +WI+V   S  S +V+ GD+  A  N R+ S
Sbjct: 187 GLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVK-PSQDSVLVMVGDSLCALLNGRLHS 246

Query: 248 PTHKVMIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEG 301
           P H+V++ G + RYS  +F    +  I++ P+ELVD+ HP  +KPF++   LHFF+++ G
Sbjct: 247 PYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEAG 300

BLAST of HG10023306.1 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.9e-58
Identity = 120/306 (39.22%), Postives = 177/306 (57.84%), Query Frame = 0

Query: 8   LPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEVLKAVKALFE 67
           LPVI+ S + +K  G+  W     +VREALEEYGCF A  + VS EL+  V      L  
Sbjct: 9   LPVIDLSMKNLK-PGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELLN 68

Query: 68  LPIETKKKNKYEKPFNGYVGELRTLP-LHESLGIDDATNFHGVHSFTHLMWPSGNHHFSA 127
           LP+ETK  N  +  ++G+     ++P L+E +G+   T+   V  FT+LMWP GN  F  
Sbjct: 69  LPLETKMLNGNKPEYDGFT----SIPNLNEGMGVGRITDLEKVERFTNLMWPEGNKDFCE 128

Query: 128 TLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQSPNPTLCLVD 187
           T++S+ K   E+D ++  M+FES+G+EK+  S  +S  YLL  ++ + P     +  L  
Sbjct: 129 TVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALSL 188

Query: 188 HTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSNDRIESPTHK 247
           H DKS  T++ Q+ + GLE +TK G+WI   + +  + +V+ GD F AWSN R+ SP H+
Sbjct: 189 HKDKSILTIVNQNDVKGLEFETKDGEWI---LPTADNHIVLLGDCFMAWSNGRLHSPLHR 248

Query: 248 VMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNSKSP 307
           V +  N+ R S + F F   I+E P ELVD+ HPL + PF+   LL +  +KEG  +   
Sbjct: 249 VTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAKAVCD 306

Query: 308 LKDYCA 313
           LK Y A
Sbjct: 309 LKQYKA 306

BLAST of HG10023306.1 vs. ExPASy Swiss-Prot
Match: Q9ZTA1 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.4e-53
Identity = 138/413 (33.41%), Postives = 193/413 (46.73%), Query Frame = 0

Query: 1   MGSETEGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEVLK 60
           MGS +  LP+I  S + +K  GS +W +    VR+ALE+YGCF A+ + VS EL+G VLK
Sbjct: 1   MGSCSPQLPLICLSDQTLK-PGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQGSVLK 60

Query: 61  AVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMWPSG 120
           A++ LF LP E K++N   KPF GY   L    L ES GI DA      H FT  +WP G
Sbjct: 61  AMQELFALPTEAKQRNVCPKPFTGY---LSHNGLSESFGIKDANILEKAHEFTQQLWPEG 120

Query: 121 NHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQSPNP 180
           N   S  +  +A+   ELD MV ++I ESYG+E +   H +S  Y +R++K  A    + 
Sbjct: 121 NKSISKMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDI 180

Query: 181 T----------------------------------------------------------- 240
           T                                                           
Sbjct: 181 TAAVGANVDNGANDNADGDANVNDDGASIGVKVNVDVGDDVNDNDSVNIGVGVDINVETN 240

Query: 241 -------------------------------------LCLVDHTDKSFTTMLYQDHINGL 300
                                                L L  HTDKS T ++YQ  I+GL
Sbjct: 241 VNGDLDAEANGDATAWVVGAVSGNASVGAKEANVDAELGLPSHTDKSLTGIIYQHQIDGL 300

Query: 301 EVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSNDRIESPTHKVMI-RGNEDRYSLAMFG- 315
           EVKTK G+WI+V   +P++ +VIAGDA  A  N RI SP H+V +    + RY+ A+F  
Sbjct: 301 EVKTKEGKWIRVK-PAPNTVIVIAGDALCALMNGRIPSPYHRVRVTEKKKTRYAAALFSN 360

BLAST of HG10023306.1 vs. ExPASy TrEMBL
Match: A0A6J1GRH7 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita moschata OX=3662 GN=LOC111456372 PE=4 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.5e-156
Identity = 271/318 (85.22%), Postives = 288/318 (90.57%), Query Frame = 0

Query: 1   MGSET-----EGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELR 60
           MGSET      GLPVINFSG+ VKAEG   WRR S +VREALEEYGCFIAEYEG+S ELR
Sbjct: 1   MGSETGPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  GEVLKAVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHL 120
            EV KAV+ALFELPIETKKKN+YEKPFNGYVGELRTLPLHESLGIDDATN  GV +FTHL
Sbjct: 61  VEVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTHL 120

Query: 121 MWPSGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAP 180
           MWP+GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKYH++HA SITYLLR LKNKAP
Sbjct: 121 MWPNGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKAP 180

Query: 181 QSPNPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAW 240
            S NPTLCLVDHTDKSFTTM+YQDH NGLEV+TK G+WIQVD SSPS+FVVIAGDAFKAW
Sbjct: 181 HSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKAW 240

Query: 241 SNDRIESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SNDRIESPTHKV IRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLHFF
Sbjct: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFF 300

Query: 301 RSKEGFNSKSPLKDYCAL 314
           RSKEGF SKSPL+DYCAL
Sbjct: 301 RSKEGFKSKSPLQDYCAL 318

BLAST of HG10023306.1 vs. ExPASy TrEMBL
Match: A0A6J1JM78 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita maxima OX=3661 GN=LOC111488145 PE=4 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.1e-155
Identity = 269/318 (84.59%), Postives = 288/318 (90.57%), Query Frame = 0

Query: 1   MGSET-----EGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELR 60
           MGSET      GLPVINFSG+ VKAEG   WRR S +VREALEEYGCFIAEYEG+S ELR
Sbjct: 1   MGSETGPRMDAGLPVINFSGDDVKAEGGEVWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  GEVLKAVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHL 120
            +V KAV+ALFELPIETKKKN+YEKPFNGYVGELRTLPLHESLGIDDATN  GV +FT+L
Sbjct: 61  VQVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTNL 120

Query: 121 MWPSGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAP 180
           MWP+GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKYH++HA SITYLLR LKNKAP
Sbjct: 121 MWPNGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKAP 180

Query: 181 QSPNPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAW 240
            S NPTLCLVDHTDKSFTTM+YQDH NGLEV+TK G+WIQVD SSPS+FVVIAGDAFKAW
Sbjct: 181 HSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKAW 240

Query: 241 SNDRIESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SNDRIESPTHKV IRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLHFF
Sbjct: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFF 300

Query: 301 RSKEGFNSKSPLKDYCAL 314
           RSKEGF SKSPL+DYCAL
Sbjct: 301 RSKEGFKSKSPLQDYCAL 318

BLAST of HG10023306.1 vs. ExPASy TrEMBL
Match: A0A6J1DBW9 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111019607 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 8.4e-150
Identity = 254/307 (82.74%), Postives = 280/307 (91.21%), Query Frame = 0

Query: 7   GLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEVLKAVKALF 66
           GLPVIN SG+ ++  G   W+ T +KVREA+EEYGCF+AEY GVS ELR +V KA++ LF
Sbjct: 30  GLPVINLSGDDLR-PGGERWKTTGKKVREAVEEYGCFMAEYNGVSPELREQVFKALEQLF 89

Query: 67  ELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMWPSGNHHFSA 126
           +LP+E K KNKYEKPFNGYVG+LRTLPLHESLGIDDAT FHGVHSFT+LMWPSGNHHFSA
Sbjct: 90  DLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSA 149

Query: 127 TLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQSPNPTLCLVD 186
           TLHSFAKIAE LDKMVTKMIFESYGVEKY+ SHA SITYLLR+LKNKAPQS NP+LCLVD
Sbjct: 150 TLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVD 209

Query: 187 HTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSNDRIESPTHK 246
           HTDKSFTTMLYQDHINGLEVKTK GQWI+V+ISSPS+F+VIAGDAFKAWSN RIESPTHK
Sbjct: 210 HTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHK 269

Query: 247 VMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNSKSP 306
           VM RGNEDRYS+ MFGFSNG VEVPKELVDQNHPL+YKPF+HIGLL FF+S+EGFNSKSP
Sbjct: 270 VMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSP 329

Query: 307 LKDYCAL 314
           LKDYCAL
Sbjct: 330 LKDYCAL 335

BLAST of HG10023306.1 vs. ExPASy TrEMBL
Match: A0A1S3CDV1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.5e-143
Identity = 252/315 (80.00%), Postives = 275/315 (87.30%), Query Frame = 0

Query: 1   MGSET--EGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEV 60
           MGSET  E LPVINFS EKVKAEGS EWRRTS+KVREALEEYGCFIA+YEGV KELR EV
Sbjct: 1   MGSETMEERLPVINFSDEKVKAEGSVEWRRTSKKVREALEEYGCFIAKYEGVGKELREEV 60

Query: 61  LKAVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMWP 120
           LKAVK LFELPIE KK NKYEKPFNGYVGELRTLPLHESLGIDDATNF+GV SF+HLMWP
Sbjct: 61  LKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNGVQSFSHLMWP 120

Query: 121 SGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQSP 180
            GNHHFSATLHSFAK AEELDKMVTKMIFESYGVEKY+ SHADSITYLLRILKNKAPQSP
Sbjct: 121 EGNHHFSATLHSFAKRAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRILKNKAPQSP 180

Query: 181 NPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSND 240
           +PTLCLVDHTDKSFTTM+YQDHINGLE+KTK+GQWIQVD SSPSSFVVIAGDAFKAWSN+
Sbjct: 181 DPTLCLVDHTDKSFTTMIYQDHINGLELKTKNGQWIQVDNSSPSSFVVIAGDAFKAWSNE 240

Query: 241 RIESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSK 300
           RI S  H+V++RG E RYS+ MF F NG +EVP+  VD  +PLRYKPF+H   L + ++ 
Sbjct: 241 RIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKAN 300

Query: 301 EGFNSKSPLKDYCAL 314
              N+ S +KDYC L
Sbjct: 301 ASHNTISRIKDYCGL 315

BLAST of HG10023306.1 vs. ExPASy TrEMBL
Match: A0A5D3CGE3 (Putative inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002390 PE=4 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 3.6e-116
Identity = 212/235 (90.21%), Postives = 221/235 (94.04%), Query Frame = 0

Query: 1   MGSET--EGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEV 60
           MGSET  E LPVINFS EKVKAEGS EWRRTS+KVREALEEYGCFIA+YEGV KELR EV
Sbjct: 1   MGSETMEERLPVINFSDEKVKAEGSVEWRRTSKKVREALEEYGCFIAKYEGVGKELREEV 60

Query: 61  LKAVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMWP 120
           LKAVK LFELPIE KK NKYEKPFNGYVGELRTLPLHESLGIDDATNF+GV SF+HLMWP
Sbjct: 61  LKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNGVQSFSHLMWP 120

Query: 121 SGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQSP 180
            GNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKY+ SHADSITYLLRILKNKAPQSP
Sbjct: 121 EGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRILKNKAPQSP 180

Query: 181 NPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFK 234
           +PTLCLVDHTDKSFTTM+YQDHINGLE+KTK+GQWIQVD SSPSSFVVIAGDAFK
Sbjct: 181 DPTLCLVDHTDKSFTTMIYQDHINGLELKTKNGQWIQVDNSSPSSFVVIAGDAFK 235

BLAST of HG10023306.1 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 4.5e-103
Identity = 168/305 (55.08%), Postives = 228/305 (74.75%), Query Frame = 0

Query: 8   LPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSK-ELRGEVLKAVKALF 67
           +P ++FS E +K  G+  W  T   +R+ALEEYGCFI + +  +  +L   V  ++  LF
Sbjct: 5   IPTLDFSREDLK-PGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLF 64

Query: 68  ELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMWPSGNHHFSA 127
           +LP +TK KNKY+KP NGYVG++  LPLHESLGID+AT+     SFT LMWP GN HFS 
Sbjct: 65  DLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNEHFSE 124

Query: 128 TLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQSPNPTLCLVD 187
            L+ +A+ A ELD+MVT+M+F+SY VEKY+  + +S TYLLR+LKN+AP + NPTL  V 
Sbjct: 125 CLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGFVT 184

Query: 188 HTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSNDRIESPTHK 247
           HTDKSFTT+L+QD +NGLE++T+ G+ I +++SSPS F+V+AGDA  AWSNDR+ SP H+
Sbjct: 185 HTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPRHQ 244

Query: 248 VMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNSKSP 307
           V++ G  DRYSL MF F+NG ++VP+EL+D  HPL YKPFDH+GLL F+R+  G+ S+ P
Sbjct: 245 VLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSECP 304

Query: 308 LKDYC 312
           +K YC
Sbjct: 305 IKAYC 308

BLAST of HG10023306.1 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 326.2 bits (835), Expect = 2.8e-89
Identity = 148/310 (47.74%), Postives = 211/310 (68.06%), Query Frame = 0

Query: 4   ETEGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEVLKAVK 63
           +T  +P+++ + ++     +  WR  SR+  EALEEYGCF+A Y+GV+++L   +  A +
Sbjct: 6   KTTKVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAE 65

Query: 64  ALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLMWPSGNHH 123
            LF+LP ETKKKN  EKP++GYVG++  +PLHE LG+D  TN      FTHLMWP GN  
Sbjct: 66  ELFDLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDR 125

Query: 124 FSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQSPNPTLC 183
           F  T+H+F+    ELD++V +MIFE+YGVEK+++SH  S TYLL+ LK  AP        
Sbjct: 126 FCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISMPA 185

Query: 184 LVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSNDRIESP 243
              HTDK+F ++L+Q+ +NGLEVK+K G+WI + +  P S+VV+AGD    WSNDRI S 
Sbjct: 186 FPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQL-PPKSYVVMAGDISMGWSNDRIRSC 245

Query: 244 THKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNS 303
            H+V + G++ RY+L +F F   +V +P+ELVD  HPL YKPFD+I L++F+ +KEG  +
Sbjct: 246 EHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREA 305

Query: 304 KSPLKDYCAL 314
            S LK YC +
Sbjct: 306 NSTLKAYCGI 314

BLAST of HG10023306.1 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 281.2 bits (718), Expect = 1.0e-75
Identity = 144/319 (45.14%), Postives = 202/319 (63.32%), Query Frame = 0

Query: 1   MGSETE----GLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRG 60
           MGSET      LPVI+FS + +K  G  EW  T   V++AL++YG F A ++ +  ELR 
Sbjct: 1   MGSETPLLPLRLPVIDFSNKNLK-PGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK 60

Query: 61  EVLKAVKALFELPIETKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVHSFTHLM 120
            V  A++ LF+LP++TK +N  +KPF+GYVG+   +PL+ES+GIDD+     V +FT  +
Sbjct: 61  SVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKL 120

Query: 121 WPSGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQ 180
           WP GN  FS T+ SF+K   ELD  + +MI ES+G++KY   H  S  YLLR++K K P 
Sbjct: 121 WPQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPD 180

Query: 181 SPNPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWS 240
           +    + L  HTDK+  T+LYQ+H+ GLEV+TK   WI+V   +  SF V+ GD+  A  
Sbjct: 181 TEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVK-PTQDSFTVMIGDSLYALL 240

Query: 241 NDRIESPTHKVMIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLHF 300
           N R+ SP H+VM+ G E RYSL +F    +  IV  P ELVD+ HP  +KPFDH+  L F
Sbjct: 241 NGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQF 300

Query: 301 FRSKEGFNSKSPLKDYCAL 314
           + ++ G  S+S LK YC +
Sbjct: 301 YYTEAGQRSQSALKTYCGI 317

BLAST of HG10023306.1 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-69
Identity = 134/317 (42.27%), Postives = 194/317 (61.20%), Query Frame = 0

Query: 4   ETEGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEVLKAVK 63
           E + LP+++FS +K+   G+  W  T   VR A+E  G F+AE+ GVS +LR  +L  +K
Sbjct: 5   EEQCLPILDFSSDKL-VRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMK 64

Query: 64  ALFELPIETKKKNKYEKPFNGYVG-ELRTLPLHESLGIDDATNFHGVHSFTHLMWPSGNH 123
            ++ LP + K KN+  K  +GY+   +    +HESLGID AT       F+ L+WP GN 
Sbjct: 65  EMYYLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWPQGND 124

Query: 124 HFSATLHSFAKIAEELDKMVTKMIFESYGVE--KYHKSHADSITYLLRILKNKAPQSPNP 183
            F  T H +A    ELD+ V +M++ESYG++  K+  SH++S  YLLR+L  +  Q+   
Sbjct: 125 PFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEA 184

Query: 184 TLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDISSPSSFVVIAGDAFKAWSNDRI 243
               V HTDKSF ++L+Q+H+ GL++KT  GQW+  +  SP+ FVV++G    AWSNDRI
Sbjct: 185 NTGFVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFN-PSPTRFVVLSGMGLTAWSNDRI 244

Query: 244 ESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEG 303
           ++  HKV++  +E RYSL  F F  G +  P+ELVD  HPLRY PF+H GLL F+ S   
Sbjct: 245 KACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYIN 304

Query: 304 FNSKSP---LKDYCALS 315
              KS    L+ YC ++
Sbjct: 305 SLKKSSEDLLQIYCGVT 319

BLAST of HG10023306.1 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 253.8 bits (647), Expect = 1.8e-67
Identity = 133/319 (41.69%), Postives = 190/319 (59.56%), Query Frame = 0

Query: 2   GSETEGLPVINFSGEKVKAEGSGEWRRTSRKVREALEEYGCFIAEYEGVSKELRGEVLKA 61
           G+ET  LP+IN S + +K + +  W  T   VREA+E +G F+AEY      L   +L+A
Sbjct: 3   GNETRELPIINLSDKNLK-QNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEA 62

Query: 62  VKALFELPIETKKKNKYEKPFNGYVGELRT-LPLHESLGIDDATNFHGVHSFTHLMWP-- 121
            K L +LP+E K KN+  K  +GY+  +    P+HE LGID   +      F+ LMWP  
Sbjct: 63  AKELLDLPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPDD 122

Query: 122 -SGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPQS 181
              N  F  T+H++AK+  EL+++V +M+FESY VEKY + +     YLLR+LK +   +
Sbjct: 123 HDDNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRLPN 182

Query: 182 PNPTLCLVDHTDKSFTTMLYQDHINGLEVKT-KHGQWIQVDISSPSSFVVIAGDAFKAWS 241
             P    + HTDKSF ++L+Q+HI GL +K+ K   W      SP+ FVVIAGDA  AWS
Sbjct: 183 GEPNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHF-TPSPTRFVVIAGDAIMAWS 242

Query: 242 NDRIESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 301
           NDRI++  HKV +   E RYSL  F F  G++  P+E+VD++HPL Y PF H GLL ++ 
Sbjct: 243 NDRIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYE 302

Query: 302 SKEGF--NSKSPLKDYCAL 314
           + E      ++  K YC +
Sbjct: 303 TLEVHLKAHRTMTKAYCGI 319