HG10022772.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10022772.1 vs. NCBI nr
Match: XP_038899717.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 653.3 bits (1684), Expect = 1.1e-183
Identity = 301/329 (91.49%), Postives = 316/329 (96.05%), Query Frame = 0

Query: 1   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLAL 60
           MEEASKE PKEMVMKSPPRLF+RKKWTKFDRNVAFYFL+LHLLCILAPFYFSWTC  LAL
Sbjct: 1   MEEASKEGPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLILHLLCILAPFYFSWTCLFLAL 60

Query: 61  GLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120
           GL+ LTG  I+VSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHR+VD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRYVD 120

Query: 121 SERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRD 180
           SERDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNNF+KVERN FMFYMKHG+PDNV D
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGHPDNVTD 180

Query: 181 LEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWG 240
           LEKQRFYRFIHKTYWLHP+ALSVLL+AIGGVPFIIWGMCVRFV +L +TSMVNSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSVLLYAIGGVPFIIWGMCVRFVFSLHVTSMVNSVCHIWG 240

Query: 241 KQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGV 300
           KQPWNTGDLSKNNWMVA+LTFGEGWHNNHHAFEYSARHGHEWWQID+GWY IKFLQVIG+
Sbjct: 241 KQPWNTGDLSKNNWMVAILTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLQVIGL 300

Query: 301 ATNVKLPSEKQKLAALNKSKPTFLKPMIN 330
           ATNVKLPSEKQKLAALNKSKPTFLKPMI+
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPTFLKPMID 329

BLAST of HG10022772.1 vs. NCBI nr
Match: XP_008451258.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 643.7 bits (1659), Expect = 8.9e-181
Identity = 297/329 (90.27%), Postives = 311/329 (94.53%), Query Frame = 0

Query: 1   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLAL 60
           MEEASKEE KEMVM SP RLFKRKKWTKFD+NVAFYFLLLHLLCILAPFYFSWTC  L+L
Sbjct: 1   MEEASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSL 60

Query: 61  GLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120
           GLANLTG  I+VSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRD 180
           SERDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNNF+KVERN FMFYMKHGY DNV D
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTD 180

Query: 181 LEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWG 240
           LEKQRFYRFIHKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV++L  TSM+NSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWG 240

Query: 241 KQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGV 300
            QPWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQIDYGWY IKFLQVIG 
Sbjct: 241 NQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGF 300

Query: 301 ATNVKLPSEKQKLAALNKSKPTFLKPMIN 330
           ATNVKLPSEKQK AALNKSKPTFLKPMI+
Sbjct: 301 ATNVKLPSEKQKWAALNKSKPTFLKPMID 329

BLAST of HG10022772.1 vs. NCBI nr
Match: XP_011659261.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KAE8646293.1 hypothetical protein Csa_016268 [Cucumis sativus])

HSP 1 Score: 638.6 bits (1646), Expect = 2.9e-179
Identity = 294/329 (89.36%), Postives = 312/329 (94.83%), Query Frame = 0

Query: 1   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLAL 60
           M+E SKE+PKEMVMKSPPRLF+RKKWTKFDRNVAFYFL LHLLCILAPFYFSWTC LLAL
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60

Query: 61  GLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120
           GL+ LTG  I+VSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRD 180
           S RDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNN++KVERN+ MFYMKHGYPDNV D
Sbjct: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180

Query: 181 LEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWG 240
           LEKQRFYRF+HKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV +L +TSMVNSVCHIWG
Sbjct: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240

Query: 241 KQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGV 300
            QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWY I+FLQVIG+
Sbjct: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300

Query: 301 ATNVKLPSEKQKLAALNKSKPTFLKPMIN 330
           ATNVKLPSEKQKLAALNKSKP FLK MI+
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 329

BLAST of HG10022772.1 vs. NCBI nr
Match: XP_011659770.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus])

HSP 1 Score: 632.5 bits (1630), Expect = 2.1e-177
Identity = 292/330 (88.48%), Postives = 314/330 (95.15%), Query Frame = 0

Query: 1   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAP-FYFSWTCFLLA 60
           ME ASKE+PKEMVM SP RLFKRKKWTKFD+NVAFYFLLLHLL ILAP FYFSW C LL+
Sbjct: 1   MEGASKEDPKEMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60

Query: 61  LGLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120
           LGLANLTG AI+VSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV
Sbjct: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120

Query: 121 DSERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVR 180
           DSERDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNN++KVERN+FMFYMKHGYPDNV 
Sbjct: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVT 180

Query: 181 DLEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIW 240
           DLEKQ+FYRFIHKTYWLHP+ALS+LL+A+GG+PFIIWG+CVRFVL+L  TSM+NSVCHIW
Sbjct: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW 240

Query: 241 GKQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIG 300
           G QPWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQID GWY IKFLQVIG
Sbjct: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG 300

Query: 301 VATNVKLPSEKQKLAALNKSKPTFLKPMIN 330
           +ATNVKLPSEKQK+AALNKSKPTFL+PMI+
Sbjct: 301 LATNVKLPSEKQKMAALNKSKPTFLRPMID 330

BLAST of HG10022772.1 vs. NCBI nr
Match: XP_008451259.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 610.9 bits (1574), Expect = 6.4e-171
Identity = 282/328 (85.98%), Postives = 304/328 (92.68%), Query Frame = 0

Query: 3   EASKEEPKEMV-MKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALG 62
           E SKE+PKE++ MKSPP+LF+RKKWTKFDRN AFYFL  HLLCILAPF FSWT  LLALG
Sbjct: 4   EVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLALG 63

Query: 63  LANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 122
           L+ L+G  I+VSYHRNLAHRSFKLPKW EYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS
Sbjct: 64  LSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 123

Query: 123 ERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDL 182
           ERDPHSPIYGFWFSQM W FDSYNLTRKVCPNYFNN++K ERN+FM YMKHGYPDNV DL
Sbjct: 124 ERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPDL 183

Query: 183 EKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGK 242
           EKQ FYRFIHKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV ++ +TSMVNSVCH+WG 
Sbjct: 184 EKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWGN 243

Query: 243 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVA 302
           QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHG EWWQIDYGWYFI+FL+VIG+A
Sbjct: 244 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGLA 303

Query: 303 TNVKLPSEKQKLAALNKSKPTFLKPMIN 330
           TNVK PSEKQKLAALNKSKPTFLKP IN
Sbjct: 304 TNVKFPSEKQKLAALNKSKPTFLKPTIN 331

BLAST of HG10022772.1 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 362.1 bits (928), Expect = 6.8e-99
Identity = 172/302 (56.95%), Postives = 210/302 (69.54%), Query Frame = 0

Query: 12  MVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTG-FAI 71
           +V K    ++  ++W   D       L +HLL +LAPF F+W    +A GL  +TG   I
Sbjct: 84  LVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGI 143

Query: 72  TVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 131
           T+S+HRNL+H++FKLPKWLEY  AY  A A+QG+PIDWVSTHR HH+F DS+RDPHSP+ 
Sbjct: 144 TLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLD 203

Query: 132 GFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFI 191
           GFWFS M W FD+  +T++                       G P+NV DLEKQ FYRF+
Sbjct: 204 GFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFL 263

Query: 192 HKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNTGDLS 251
             TY LHP+AL+V L+A+GG PFI+WGM VR V    IT +VNS CH+WGKQ WNTGDLS
Sbjct: 264 RTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLS 323

Query: 252 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEK 311
           KNNW VA L FGEGWHNNHHAFE+SARHG EWWQ+D  WY +KFLQ IG+AT+VKLPSE 
Sbjct: 324 KNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEA 363

Query: 312 QK 313
           QK
Sbjct: 384 QK 363

BLAST of HG10022772.1 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 323.6 bits (828), Expect = 2.7e-87
Identity = 153/301 (50.83%), Postives = 194/301 (64.45%), Query Frame = 0

Query: 11  EMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTGFAI 70
           E+V+    R F  + WT +D +    F+  HLL +LAPFYFSW  F +   L  + G  I
Sbjct: 76  EVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICI 135

Query: 71  TVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 130
           T+SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  +++RDPHSP  
Sbjct: 136 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 195

Query: 131 GFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFI 190
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRF+
Sbjct: 196 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 255

Query: 191 HKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNTGDLS 250
            +T  LH +A S L +  GG+P ++WG+ +   + L +T +VNSVCHIWG + WNT D S
Sbjct: 256 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 315

Query: 251 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEK 310
           KNNW VA+LT GEGWHNNHHAFE+SARHG EWWQ+D  W  I+FL+ IG+ATNVKLP+E 
Sbjct: 316 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

Query: 311 Q 312
           Q
Sbjct: 376 Q 354

BLAST of HG10022772.1 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.3e-85
Identity = 151/297 (50.84%), Postives = 192/297 (64.65%), Query Frame = 0

Query: 24  KKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTG-FAITVSYHRNLAHRS 83
           +KW   D     +   LH+LC+ AP  FSW  F +   L  + G F  T+S+HRNL HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 84  FKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMMWFFD 143
           FKLPK+LEY  AY+  HA+QGDP+ WVSTHR HH++ D+  DPHSPI GFWF  + W FD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 144 SYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFIHKTYWLHPIALS 203
           S  +  K C  Y                     +N  DL KQ +YRF+ +T+  H    +
Sbjct: 158 SKYIIEK-CGRY---------------------ENAGDLMKQSYYRFLERTFVYHVYLQA 217

Query: 204 VLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNTGDLSKNNWMVALLTFG 263
            LL+  GG PFI+WGM VR +L   ++ +VNSVCH WG +PWNTGDLS NNW +A+LT G
Sbjct: 218 ALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSG 277

Query: 264 EGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSE--KQKLAALN 318
           EGWHNNHHAFEYSARHG EWWQID  WY IK L+ +G+AT++K+PSE  K+K++  N
Sbjct: 278 EGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMSFKN 312

BLAST of HG10022772.1 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.1e-84
Identity = 148/311 (47.59%), Postives = 193/311 (62.06%), Query Frame = 0

Query: 2   EEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALG 61
           E   K    +  M    R    +KW + D   AF  L +H LC+LAPF F+W    +AL 
Sbjct: 10  ENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALI 69

Query: 62  LANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 121
           +  + G  ITVSYHRNLAHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR HH+F DS
Sbjct: 70  VYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDS 129

Query: 122 ERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDL 181
           +RDPHSP  GFWFS ++W FD+  L  K                       G   NV DL
Sbjct: 130 DRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GRRTNVEDL 189

Query: 182 EKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGK 241
           ++Q +Y+F+ +T   H +    LL+  GG+ F+ WGM +   +   +T ++NS+CH+WG 
Sbjct: 190 KRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGS 249

Query: 242 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVA 301
           + W T D S+N W +++ +FGE WHNNHHAFE SAR G EWWQID  WY ++FL++IG+A
Sbjct: 250 RTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLA 298

Query: 302 TNVKLPSEKQK 313
           T+VKLPSE Q+
Sbjct: 310 TDVKLPSESQR 298

BLAST of HG10022772.1 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.1e-84
Identity = 154/296 (52.03%), Postives = 194/296 (65.54%), Query Frame = 0

Query: 22  KRKKWTK-FDRNVAFYFLL---LHLLCILAPFYFSWTCFLLALGLANLTG-FAITVSYHR 81
           K+  W + ++    F+ +L   LH+LC+ APF FSW+ F L+L L  + G F  T+SYHR
Sbjct: 29  KKITWVRVWNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHR 88

Query: 82  NLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQ 141
           NL HRSFKLPK+LEY  AY+  HA+QGDP+ WVSTHR HH+F D+  DPHSPI GFWFS 
Sbjct: 89  NLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSH 148

Query: 142 MMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFIHKTYWL 201
           + W FDS  +  + C  Y                     +N  DL KQ +YRF+ +T+  
Sbjct: 149 IFWLFDSKYILEE-CGRY---------------------ENAGDLLKQSYYRFLERTFVF 208

Query: 202 HPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNTGDLSKNNWMV 261
           H    + LL+  GG PFI+WGM VR V     + +VNSVCH +G Q W+TGDLS NNW +
Sbjct: 209 HVYLQAALLYMFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFI 268

Query: 262 ALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEKQK 313
           A+LT GEGWHNNHHAFEYSARHG EWWQID  WY IK L+ +G+AT+VK+PSE  K
Sbjct: 269 AMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYVIKLLEYLGLATDVKVPSEVHK 302

BLAST of HG10022772.1 vs. ExPASy TrEMBL
Match: A0A1S3BS49 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492608 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 4.3e-181
Identity = 297/329 (90.27%), Postives = 311/329 (94.53%), Query Frame = 0

Query: 1   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLAL 60
           MEEASKEE KEMVM SP RLFKRKKWTKFD+NVAFYFLLLHLLCILAPFYFSWTC  L+L
Sbjct: 1   MEEASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSL 60

Query: 61  GLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120
           GLANLTG  I+VSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRD 180
           SERDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNNF+KVERN FMFYMKHGY DNV D
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTD 180

Query: 181 LEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWG 240
           LEKQRFYRFIHKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV++L  TSM+NSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWG 240

Query: 241 KQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGV 300
            QPWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQIDYGWY IKFLQVIG 
Sbjct: 241 NQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGF 300

Query: 301 ATNVKLPSEKQKLAALNKSKPTFLKPMIN 330
           ATNVKLPSEKQK AALNKSKPTFLKPMI+
Sbjct: 301 ATNVKLPSEKQKWAALNKSKPTFLKPMID 329

BLAST of HG10022772.1 vs. ExPASy TrEMBL
Match: A0A0A0KAB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.1e-180
Identity = 295/329 (89.67%), Postives = 313/329 (95.14%), Query Frame = 0

Query: 1   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLAL 60
           M+E SKE+PKEMVMKSPPRLF+RKKWTKFDRNVAFYFL LHLLCILAPFYFSWTC LLAL
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60

Query: 61  GLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120
           GL+ LTG  I+VSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRD 180
           S RDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNN++KVERN+FMFYMKHGYPDNV D
Sbjct: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180

Query: 181 LEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWG 240
           LEKQ+FYRFIHKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV +L +TSMVNSVCHIWG
Sbjct: 181 LEKQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240

Query: 241 KQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGV 300
            QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWY I+FLQVIG+
Sbjct: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300

Query: 301 ATNVKLPSEKQKLAALNKSKPTFLKPMIN 330
           ATNVKLPSEKQKLAALNKSKP FLK MI+
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 329

BLAST of HG10022772.1 vs. ExPASy TrEMBL
Match: A0A1S3BQG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492609 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.1e-171
Identity = 282/328 (85.98%), Postives = 304/328 (92.68%), Query Frame = 0

Query: 3   EASKEEPKEMV-MKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALG 62
           E SKE+PKE++ MKSPP+LF+RKKWTKFDRN AFYFL  HLLCILAPF FSWT  LLALG
Sbjct: 4   EVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLALG 63

Query: 63  LANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 122
           L+ L+G  I+VSYHRNLAHRSFKLPKW EYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS
Sbjct: 64  LSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 123

Query: 123 ERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDL 182
           ERDPHSPIYGFWFSQM W FDSYNLTRKVCPNYFNN++K ERN+FM YMKHGYPDNV DL
Sbjct: 124 ERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPDL 183

Query: 183 EKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGK 242
           EKQ FYRFIHKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV ++ +TSMVNSVCH+WG 
Sbjct: 184 EKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWGN 243

Query: 243 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVA 302
           QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHG EWWQIDYGWYFI+FL+VIG+A
Sbjct: 244 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGLA 303

Query: 303 TNVKLPSEKQKLAALNKSKPTFLKPMIN 330
           TNVK PSEKQKLAALNKSKPTFLKP IN
Sbjct: 304 TNVKFPSEKQKLAALNKSKPTFLKPTIN 331

BLAST of HG10022772.1 vs. ExPASy TrEMBL
Match: A0A6J1GPG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456275 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.5e-165
Identity = 272/319 (85.27%), Postives = 296/319 (92.79%), Query Frame = 0

Query: 3   EASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGL 62
           EASK+ P   +++SPP LF RKKWTKFDRNVA YFLLLHLLCILAPFYF+WT F L + L
Sbjct: 2   EASKQHPN--MVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTSFFLTIIL 61

Query: 63  ANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122
           ANLTG AI+VSYHRNLAHR+FKLPKWLEYSLAY AAHAIQGDPIDWVSTHRCHHR+VD+E
Sbjct: 62  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 121

Query: 123 RDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLE 182
           RDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYF NFEKVERNMFMF+MKHGYPDNVRDLE
Sbjct: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 181

Query: 183 KQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQ 242
           KQ FYRF+ KTYWLHP+AL+ LL+A+GGVP++IWGMCVRFVL+L ITSMVNSVCHIWG Q
Sbjct: 182 KQWFYRFMRKTYWLHPLALAGLLYAVGGVPYVIWGMCVRFVLSLHITSMVNSVCHIWGTQ 241

Query: 243 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVAT 302
           PWNTGDLS+NNWMVAL+TFGEGWHNNHHAFEYSARHGHEWW+IDYGWY IKFLQVIG+AT
Sbjct: 242 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWEIDYGWYVIKFLQVIGLAT 301

Query: 303 NVKLPSEKQKLAALNKSKP 322
            VKLPS+ QKLAALNKSKP
Sbjct: 302 CVKLPSQTQKLAALNKSKP 318

BLAST of HG10022772.1 vs. ExPASy TrEMBL
Match: A0A6J1JUL6 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488510 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.3e-165
Identity = 270/319 (84.64%), Postives = 294/319 (92.16%), Query Frame = 0

Query: 3   EASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGL 62
           EASK+ P   +++SPP LF RKKWTKFDRNVA YFLLLHLLCILAPFYF+WTCF L + L
Sbjct: 2   EASKQHPN--MVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTCFFLTIIL 61

Query: 63  ANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122
           ANLTG AI+VSYHRNLAHR+FKLPKWLEYSLAY AAHAIQGDPIDWVSTHRCHHR+VD+E
Sbjct: 62  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 121

Query: 123 RDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLE 182
           RDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYF NFEKVERNMFMF+MKHGYPDNVRDLE
Sbjct: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 181

Query: 183 KQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQ 242
           KQ FYRF+ KTYWLHP+AL+ LL+A GG+P++IWGMCVRFVL+L +TSMVNSVCHIWG Q
Sbjct: 182 KQWFYRFMRKTYWLHPLALAGLLYAAGGIPYVIWGMCVRFVLSLHVTSMVNSVCHIWGNQ 241

Query: 243 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVAT 302
           PWNTGDLS+NNWMVAL+TFGEGWHNNHHAFEYSARHGHEWWQIDYGWY IKFLQVIG+AT
Sbjct: 242 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT 301

Query: 303 NVKLPSEKQKLAALNKSKP 322
            VKLPS+   LAALNKSKP
Sbjct: 302 CVKLPSQTHNLAALNKSKP 318

BLAST of HG10022772.1 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 362.1 bits (928), Expect = 4.8e-100
Identity = 172/302 (56.95%), Postives = 210/302 (69.54%), Query Frame = 0

Query: 12  MVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTG-FAI 71
           +V K    ++  ++W   D       L +HLL +LAPF F+W    +A GL  +TG   I
Sbjct: 84  LVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGI 143

Query: 72  TVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 131
           T+S+HRNL+H++FKLPKWLEY  AY  A A+QG+PIDWVSTHR HH+F DS+RDPHSP+ 
Sbjct: 144 TLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLD 203

Query: 132 GFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFI 191
           GFWFS M W FD+  +T++                       G P+NV DLEKQ FYRF+
Sbjct: 204 GFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFL 263

Query: 192 HKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNTGDLS 251
             TY LHP+AL+V L+A+GG PFI+WGM VR V    IT +VNS CH+WGKQ WNTGDLS
Sbjct: 264 RTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLS 323

Query: 252 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEK 311
           KNNW VA L FGEGWHNNHHAFE+SARHG EWWQ+D  WY +KFLQ IG+AT+VKLPSE 
Sbjct: 324 KNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEA 363

Query: 312 QK 313
           QK
Sbjct: 384 QK 363

BLAST of HG10022772.1 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 323.6 bits (828), Expect = 1.9e-88
Identity = 153/301 (50.83%), Postives = 194/301 (64.45%), Query Frame = 0

Query: 11  EMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTGFAI 70
           E+V+    R F  + WT +D +    F+  HLL +LAPFYFSW  F +   L  + G  I
Sbjct: 76  EVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICI 135

Query: 71  TVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 130
           T+SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  +++RDPHSP  
Sbjct: 136 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 195

Query: 131 GFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFI 190
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRF+
Sbjct: 196 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 255

Query: 191 HKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNTGDLS 250
            +T  LH +A S L +  GG+P ++WG+ +   + L +T +VNSVCHIWG + WNT D S
Sbjct: 256 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 315

Query: 251 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEK 310
           KNNW VA+LT GEGWHNNHHAFE+SARHG EWWQ+D  W  I+FL+ IG+ATNVKLP+E 
Sbjct: 316 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

Query: 311 Q 312
           Q
Sbjct: 376 Q 354

BLAST of HG10022772.1 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 312.0 bits (798), Expect = 5.7e-85
Identity = 148/311 (47.59%), Postives = 193/311 (62.06%), Query Frame = 0

Query: 2   EEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALG 61
           E   K    +  M    R    +KW + D   AF  L +H LC+LAPF F+W    +AL 
Sbjct: 10  ENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALI 69

Query: 62  LANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 121
           +  + G  ITVSYHRNLAHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR HH+F DS
Sbjct: 70  VYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDS 129

Query: 122 ERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDL 181
           +RDPHSP  GFWFS ++W FD+  L  K                       G   NV DL
Sbjct: 130 DRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GRRTNVEDL 189

Query: 182 EKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGK 241
           ++Q +Y+F+ +T   H +    LL+  GG+ F+ WGM +   +   +T ++NS+CH+WG 
Sbjct: 190 KRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGS 249

Query: 242 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVA 301
           + W T D S+N W +++ +FGE WHNNHHAFE SAR G EWWQID  WY ++FL++IG+A
Sbjct: 250 RTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLA 298

Query: 302 TNVKLPSEKQK 313
           T+VKLPSE Q+
Sbjct: 310 TDVKLPSESQR 298

BLAST of HG10022772.1 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 311.6 bits (797), Expect = 7.5e-85
Identity = 146/293 (49.83%), Postives = 188/293 (64.16%), Query Frame = 0

Query: 20  LFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTGFAITVSYHRNLA 79
           +F  ++W + D         +H L +LAPFYF+W+   +      + G  ITVSYHRNLA
Sbjct: 30  VFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLA 89

Query: 80  HRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMMW 139
           HRSFK+PKWLEY LAY A  AIQGDPIDWVSTHR HH+F DSERDPHSP  GFWFS ++W
Sbjct: 90  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 149

Query: 140 FFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFIHKTYWLHPI 199
            +DS  L  K                       G   NV DL++Q FYRF+ KT   H +
Sbjct: 150 IYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHIL 209

Query: 200 ALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNTGDLSKNNWMVALL 259
            L   LF +GG+ F+ WGM V   L + +T ++NS+CHIWG + W T D S+N W +++ 
Sbjct: 210 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVF 269

Query: 260 TFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEKQK 313
           +FGE WHNNHHAFE SAR G EWWQID  WY ++F ++IG+AT+VK+P+E Q+
Sbjct: 270 SFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQR 300

BLAST of HG10022772.1 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 307.0 bits (785), Expect = 1.8e-83
Identity = 155/314 (49.36%), Postives = 199/314 (63.38%), Query Frame = 0

Query: 1   MEEASKEE--PKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLL 60
           M E +K++   ++  ++   R +  +KWT+FD   A     +HLLC+LAPF + W  F  
Sbjct: 1   MSETTKDDGSSQKKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRF 60

Query: 61  ALGLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRF 120
            + LA LT   IT SYHRNL HRSFKLPKWLEY  AY A  A+QGDP+DWVS HR HH+F
Sbjct: 61  GIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQF 120

Query: 121 VDSERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNV 180
            DS+RDPHSPI GFWFS ++W FD+ +  R+ C                     G  +NV
Sbjct: 121 TDSDRDPHSPIEGFWFSHVLWIFDT-DYIREKC---------------------GRRNNV 180

Query: 181 RDLEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHI 240
            DL++Q FYRF+ KT  LH +A   L++  GG+P++ W +    V+    T +VNS CHI
Sbjct: 181 MDLKQQWFYRFLKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHI 240

Query: 241 WGKQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVI 300
            G Q W T D S+N W +ALLT GE WHNNHHAFE SARHG EW+Q+D  WY I F Q +
Sbjct: 241 CGSQAWQTNDTSRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQAL 292

Query: 301 GVATNVKLPSEKQK 313
           G+ATNVKLP++ QK
Sbjct: 301 GLATNVKLPTDAQK 292