HG10021665.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10021665.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Two-pore potassium channel 1-like
Location	Chr05: 13466505 .. 13467687 (-)
Sequence length	1038
RNA-Seq Expression	HG10021665.1
Synteny	HG10021665.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTCTGGTGATGTTGAGCAATCCTTGCTTCCTAAGCCGTCCGATTCTGATAAGATGAATTCTCTCCTGAGAAGAAAATCTAACCGTCATGGTAGTTTCTCACATTCAGTAGAAAATAATAATCAACCACAAAATTATGATGCAGTGTCTCATCAACGTATTGCTGTTTCACACGTTAGTTTTAGGAAAGTATTTGTTCTTCTGGCAACTTATTTGGGAGGAGGCACGTTTTGTTTTTTCCTTGTTCGGGATCAGATCACTGGCAAGAAAACAAATGGGATTGTTGATGCCATTTACTTTTGTGTTGTGACAATGACCACCGTTGGGTATGGTGATCTTGTTCCTAATAGCACAGTTGCGAAACTACTTGCATGTGCTTATGTCTTCACTGGAATGACTCTTGGTGGGATGATTCTTAGCAAGGCAGCCGATTACATTGTGGAAAAGCAGGAAATTCTTCTGGTTAAGGCCATGTACATGAGGAAAAAGATCAGCTCCAGTGAGATCCTTCAGGAATCTGAGGCTCACAAATTGAAATACAAATTCATTATGACTGGAATCCTCCTTTGGGCTCTTGTCGTTGTTGGAACTCTTTTCTTGACTGTGGTTGAGAAGCTAGAGTTCACAGATGCCTTTTATTGCGTTTGTTCCACAATCACGACTCTTGGCTATGGAGATGAGAGTTTCTCTACAACAGCTGGTCGTGTTTTTGCTGTCATTTGGATAATGAGCAGTACCATCTGCCTAGCACAGTTCTTTCTGTACCTTGCAGAATTACACACAGAAAGAAGACAGGAATCACTGGTAAATTGGGTTCTCAGCCGAAGCCTCACGTACTCGGATCTTGAAGAAGCAGACCTTGATCATGATAAAGTAGTAAGGTCAGCCTGATTTACGTTCATATTTCTTTTATCCTTGCGTTCCACTGGATTTATTTTCCATTACCCATGGCTTATCAATGACTAATCCTATTTTGATTCAATTCTTGCATGTATCATGGGGCTTATTGGCAATCTGCTTTATCAGTGCTGCAGAATTTGTTATATATAAACTGAAGGAAATGGGGAAGATCAATCAGGAAGACGTTTCTCCCATTCTCGACACTTTTAAGAAGCTTGACATTGATCAATCAGGATGTTTAACAGAAGCTGATATAGTAACCTCATCGTCGTTGGCTTAA

mRNA sequence

ATGGACTCTGGTGATGTTGAGCAATCCTTGCTTCCTAAGCCGTCCGATTCTGATAAGATGAATTCTCTCCTGAGAAGAAAATCTAACCGTCATGGTAGTTTCTCACATTCAGTAGAAAATAATAATCAACCACAAAATTATGATGCAGTGTCTCATCAACGTATTGCTGTTTCACACGTTAGTTTTAGGAAAGTATTTGTTCTTCTGGCAACTTATTTGGGAGGAGGCACGTTTTGTTTTTTCCTTGTTCGGGATCAGATCACTGGCAAGAAAACAAATGGGATTGTTGATGCCATTTACTTTTGTGTTGTGACAATGACCACCGTTGGGTATGGTGATCTTGTTCCTAATAGCACAGTTGCGAAACTACTTGCATGTGCTTATGTCTTCACTGGAATGACTCTTGGTGGGATGATTCTTAGCAAGGCAGCCGATTACATTGTGGAAAAGCAGGAAATTCTTCTGGTTAAGGCCATGTACATGAGGAAAAAGATCAGCTCCAGTGAGATCCTTCAGGAATCTGAGGCTCACAAATTGAAATACAAATTCATTATGACTGGAATCCTCCTTTGGGCTCTTGTCGTTGTTGGAACTCTTTTCTTGACTGTGGTTGAGAAGCTAGAGTTCACAGATGCCTTTTATTGCGTTTGTTCCACAATCACGACTCTTGGCTATGGAGATGAGAGTTTCTCTACAACAGCTGGTCGTGTTTTTGCTGTCATTTGGATAATGAGCAGTACCATCTGCCTAGCACAGTTCTTTCTGTACCTTGCAGAATTACACACAGAAAGAAGACAGGAATCACTGGTAAATTGGGTTCTCAGCCGAAGCCTCACGTACTCGGATCTTGAAGAAGCAGACCTTGATCATGATAAAGTAGTAAGTGCTGCAGAATTTGTTATATATAAACTGAAGGAAATGGGGAAGATCAATCAGGAAGACGTTTCTCCCATTCTCGACACTTTTAAGAAGCTTGACATTGATCAATCAGGATGTTTAACAGAAGCTGATATAGTAACCTCATCGTCGTTGGCTTAA

Coding sequence (CDS)

ATGGACTCTGGTGATGTTGAGCAATCCTTGCTTCCTAAGCCGTCCGATTCTGATAAGATGAATTCTCTCCTGAGAAGAAAATCTAACCGTCATGGTAGTTTCTCACATTCAGTAGAAAATAATAATCAACCACAAAATTATGATGCAGTGTCTCATCAACGTATTGCTGTTTCACACGTTAGTTTTAGGAAAGTATTTGTTCTTCTGGCAACTTATTTGGGAGGAGGCACGTTTTGTTTTTTCCTTGTTCGGGATCAGATCACTGGCAAGAAAACAAATGGGATTGTTGATGCCATTTACTTTTGTGTTGTGACAATGACCACCGTTGGGTATGGTGATCTTGTTCCTAATAGCACAGTTGCGAAACTACTTGCATGTGCTTATGTCTTCACTGGAATGACTCTTGGTGGGATGATTCTTAGCAAGGCAGCCGATTACATTGTGGAAAAGCAGGAAATTCTTCTGGTTAAGGCCATGTACATGAGGAAAAAGATCAGCTCCAGTGAGATCCTTCAGGAATCTGAGGCTCACAAATTGAAATACAAATTCATTATGACTGGAATCCTCCTTTGGGCTCTTGTCGTTGTTGGAACTCTTTTCTTGACTGTGGTTGAGAAGCTAGAGTTCACAGATGCCTTTTATTGCGTTTGTTCCACAATCACGACTCTTGGCTATGGAGATGAGAGTTTCTCTACAACAGCTGGTCGTGTTTTTGCTGTCATTTGGATAATGAGCAGTACCATCTGCCTAGCACAGTTCTTTCTGTACCTTGCAGAATTACACACAGAAAGAAGACAGGAATCACTGGTAAATTGGGTTCTCAGCCGAAGCCTCACGTACTCGGATCTTGAAGAAGCAGACCTTGATCATGATAAAGTAGTAAGTGCTGCAGAATTTGTTATATATAAACTGAAGGAAATGGGGAAGATCAATCAGGAAGACGTTTCTCCCATTCTCGACACTTTTAAGAAGCTTGACATTGATCAATCAGGATGTTTAACAGAAGCTGATATAGTAACCTCATCGTCGTTGGCTTAA

Protein sequence

MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHVSFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTVAKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLKYKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAVIWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFVIYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTSSSLA

Homology

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BLAST of HG10021665.1 vs. NCBI nr
Match: XP_038877797.1 (two-pore potassium channel 1-like [Benincasa hispida])

HSP 1 Score: 614.4 bits (1583), Expect = 6.1e-172
Identity = 323/341 (94.72%), Postives = 326/341 (95.60%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHV 60
           MDSGDVEQSLLPK SDSDK NSL RRKSNRHGSFSHSVENNNQP NYD VSHQRIAVS V
Sbjct: 1   MDSGDVEQSLLPKLSDSDKRNSLQRRKSNRHGSFSHSVENNNQPHNYDVVSHQRIAVSQV 60

Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFRKVFVLLA YLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNS V
Sbjct: 61  SFRKVFVLLAIYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIV 120

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLAC YVFTGMTLGGMILSKAADYIVEKQEILLVKAMYM KKISSSEILQESEA+KLK
Sbjct: 121 AKLLACVYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMGKKISSSEILQESEANKLK 180

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           +KFIMTGILLW LVVVGTL LTVVEKLEF DAFYCVCSTITTLGYGDESFSTTAGRVFAV
Sbjct: 181 HKFIMTGILLWGLVVVGTLILTVVEKLEFMDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
           IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTY DLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYKDLEEADLDHDKVVSAAEFV 300

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTS 342
           +YKLKEMGKINQEDVSPILDTFKKLDIDQSG LTEADIVTS
Sbjct: 301 LYKLKEMGKINQEDVSPILDTFKKLDIDQSGSLTEADIVTS 341

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BLAST of HG10021665.1 vs. NCBI nr
Match: XP_004139602.1 (two-pore potassium channel 1 [Cucumis sativus] >KGN64997.1 hypothetical protein Csa_022708 [Cucumis sativus])

HSP 1 Score: 613.6 bits (1581), Expect = 1.0e-171
Identity = 321/342 (93.86%), Postives = 328/342 (95.91%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHV 60
           MDS D EQSLLPK SDSDK NSLLRRKSNRHGSFSHSVENNNQPQNYD VSHQRIAVS V
Sbjct: 1   MDSDDFEQSLLPKLSDSDKKNSLLRRKSNRHGSFSHSVENNNQPQNYDVVSHQRIAVSQV 60

Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNG+VD+IYFCVVTMTTVGYGDLVP+S V
Sbjct: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGVVDSIYFCVVTMTTVGYGDLVPDSMV 120

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLAC YVFTGMTLGGMILSKAADYIVEKQEILLVKAM MRKKISSSEILQESEA+KLK
Sbjct: 121 AKLLACVYVFTGMTLGGMILSKAADYIVEKQEILLVKAMCMRKKISSSEILQESEANKLK 180

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YKFIMTGILLWAL+VVG LFLTVVE LEFTDAFYCVCSTITTLGYGD+SFSTTAGRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGILFLTVVENLEFTDAFYCVCSTITTLGYGDQSFSTTAGRVFAV 240

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
           IWIMS TICLAQFFLYLAEL+TERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSGTICLAQFFLYLAELYTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTSS 343
           IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIV  S
Sbjct: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVVIS 342

[Close]

BLAST of HG10021665.1 vs. NCBI nr
Match: XP_016903168.1 (PREDICTED: LOW QUALITY PROTEIN: two-pore potassium channel 1-like [Cucumis melo])

HSP 1 Score: 597.4 bits (1539), Expect = 7.7e-167
Identity = 314/341 (92.08%), Postives = 326/341 (95.60%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHV 60
           MDSGDVEQSLL K  DSDK NSLLRRKSNRHGSFSHSVE NN+PQNYD V+HQR+AVS +
Sbjct: 1   MDSGDVEQSLLLKLFDSDKRNSLLRRKSNRHGSFSHSVE-NNKPQNYD-VAHQRVAVSQI 60

Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFRKVFVLLATYLGGGTFCF LVRDQITGKKTNG+VD+IYFCVVTMTTVGYGDLVPNS V
Sbjct: 61  SFRKVFVLLATYLGGGTFCFXLVRDQITGKKTNGVVDSIYFCVVTMTTVGYGDLVPNSMV 120

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLAC YVFTGMTLGGMILSKAADYIVEKQEILLVKAM MRKKISSSEILQESE +KLK
Sbjct: 121 AKLLACVYVFTGMTLGGMILSKAADYIVEKQEILLVKAMRMRKKISSSEILQESEENKLK 180

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YKFIMTGILLWAL+VVGTLFLTVVE LEFTDAFYCVCSTITTLGYGD+SFSTTAGRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGTLFLTVVENLEFTDAFYCVCSTITTLGYGDQSFSTTAGRVFAV 240

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
           IWIMS TICLAQFFLYLAELHTERRQ+SL+NWVL+RSLTYSDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSGTICLAQFFLYLAELHTERRQDSLINWVLNRSLTYSDLEEADLDHDKVVSAAEFV 300

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTS 342
           IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIV S
Sbjct: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVIS 339

[Close]

BLAST of HG10021665.1 vs. NCBI nr
Match: XP_022142571.1 (two-pore potassium channel 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 558.5 bits (1438), Expect = 4.0e-155
Identity = 292/341 (85.63%), Postives = 311/341 (91.20%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHV 60
           MD GDVEQSLLP  +DS K N+L RRKS R GSFS  VENNNQPQ  D VSHQ      +
Sbjct: 1   MDLGDVEQSLLPNLADSGKRNALQRRKSARRGSFSQLVENNNQPQICDEVSHQSTIAQQI 60

Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFR+VFVLLATYLGGGT CFFLV+DQI+GKKTNGIVDA+YFCVVTMTTVGYGDLVP+S V
Sbjct: 61  SFRQVFVLLATYLGGGTLCFFLVQDQISGKKTNGIVDALYFCVVTMTTVGYGDLVPSSLV 120

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVK MYMR  ISSSEI+QESEAHKLK
Sbjct: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKTMYMRNNISSSEIVQESEAHKLK 180

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YKFIMTGILLWAL+VVGTLFLT VEKLEF DAFYCVCSTITTLGYGDESFST+AGRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGTLFLTAVEKLEFMDAFYCVCSTITTLGYGDESFSTSAGRVFAV 240

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
           IWI+SSTICLAQFFLYLAEL TERRQ+SLVNWVL+R+LT+ DLEEADLDHDKVVSAAEFV
Sbjct: 241 IWILSSTICLAQFFLYLAELRTERRQKSLVNWVLNRNLTHLDLEEADLDHDKVVSAAEFV 300

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTS 342
           +YKLKEMGKI QED+SPILDTFKKLDIDQSG LTEAD+VTS
Sbjct: 301 LYKLKEMGKICQEDISPILDTFKKLDIDQSGSLTEADLVTS 341

[Close]

BLAST of HG10021665.1 vs. NCBI nr
Match: XP_022142569.1 (two-pore potassium channel 1-like isoform X1 [Momordica charantia] >XP_022142570.1 two-pore potassium channel 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 558.1 bits (1437), Expect = 5.2e-155
Identity = 292/341 (85.63%), Postives = 311/341 (91.20%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHV 60
           MD GDVEQSLLP  +DS K N+L RRKS R GSFS  VENNNQPQ  D VSHQ      +
Sbjct: 1   MDLGDVEQSLLPNLADSGKRNALQRRKSARSGSFSQLVENNNQPQICDEVSHQSTIAQQI 60

Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFR+VFVLLATYLGGGT CFFLV+DQI+GKKTNGIVDA+YFCVVTMTTVGYGDLVP+S V
Sbjct: 61  SFRQVFVLLATYLGGGTLCFFLVQDQISGKKTNGIVDALYFCVVTMTTVGYGDLVPSSLV 120

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVK MYMR  ISSSEI+QESEAHKLK
Sbjct: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKTMYMRNNISSSEIVQESEAHKLK 180

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YKFIMTGILLWAL+VVGTLFLT VEKLEF DAFYCVCSTITTLGYGDESFST+AGRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGTLFLTAVEKLEFMDAFYCVCSTITTLGYGDESFSTSAGRVFAV 240

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
           IWI+SSTICLAQFFLYLAEL TERRQ+SLVNWVL+R+LT+ DLEEADLDHDKVVSAAEFV
Sbjct: 241 IWILSSTICLAQFFLYLAELRTERRQKSLVNWVLNRNLTHLDLEEADLDHDKVVSAAEFV 300

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTS 342
           +YKLKEMGKI QED+SPILDTFKKLDIDQSG LTEAD+VTS
Sbjct: 301 LYKLKEMGKICQEDISPILDTFKKLDIDQSGSLTEADLVTS 341

[Close]

BLAST of HG10021665.1 vs. ExPASy Swiss-Prot
Match: Q8LBL1 (Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2)

HSP 1 Score: 352.8 bits (904), Expect = 4.3e-96
Identity = 172/283 (60.78%), Postives = 232/283 (81.98%), Query Frame = 0


Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           + R+V + LA YL  GT CF+LVRDQI+G KT+G+VDA+YFC+VTMTTVGYGDLVPNS+ 
Sbjct: 75  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 134

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           ++LLACA+VF+GM L G +LS+AADY+VEKQE LLV+A ++R+    ++IL+E   +KL+
Sbjct: 135 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLR 194

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YK   T ++L  L +VGT+FL +VEK+    AFYCVCST+TTLGYGD+SF++ AGR+FAV
Sbjct: 195 YKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAV 254

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
            WI++S+ICLAQFFLY+AEL+TE +Q +LV WVL+R +T +DLE ADLD D VV AAEF+
Sbjct: 255 FWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFI 314

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTSSS 344
           +YKLKEMGKI+++D+S I+D F++LD D+SG LT +DIV + +
Sbjct: 315 VYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQT 357

[Close]

BLAST of HG10021665.1 vs. ExPASy Swiss-Prot
Match: Q850M0 (Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE=1 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.3e-87
Identity = 177/350 (50.57%), Postives = 237/350 (67.71%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVEN------NNQPQNYDAVSHQR 60
           MD   ++QSLL     +D  N L R+ S     F               P+   +V  + 
Sbjct: 1   MDDNSIQQSLL-----ADNPNVLQRKPSEGVNRFRRCRSTPSTDPLQGPPEKGSSVKAKE 60

Query: 61  I-AVSHVSFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGD 120
           +      SFR V +LL  YL  G   F+ V D+I+GK+TN ++DA+YFCVVTMTTVGYGD
Sbjct: 61  LFKEMRPSFRLVGLLLFIYLLVGVLAFYAVMDEISGKRTNRVLDALYFCVVTMTTVGYGD 120

Query: 121 LVPNSTVAKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQE 180
           LVPN+   KLLACA+VF GM +  + +SK ADY+VEKQE+L  KA++   K   +++L+ 
Sbjct: 121 LVPNNDTTKLLACAFVFMGMAVVALFVSKVADYLVEKQEVLFFKALHTNLKGGETKMLRA 180

Query: 181 SEAHKLKYKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTT 240
            E +++KYKF    +LL   ++ GT+FL  VEKL   D+FYCVC+TITTLGYGD+SFS+ 
Sbjct: 181 IETNRIKYKFYTNALLLVLSIISGTVFLWKVEKLSLVDSFYCVCATITTLGYGDKSFSSK 240

Query: 241 AGRVFAVIWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKV 300
            GRVFAV WI++STI +AQFF+YLAE++TERRQ+ L NWVL+R +T  DLE ADLD D+ 
Sbjct: 241 LGRVFAVFWIITSTIIMAQFFMYLAEIYTERRQKMLANWVLTRKMTKMDLEAADLDDDRQ 300

Query: 301 VSAAEFVIYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTSSS 344
           V AAEFV+YKLKE+GKINQE++S  L+ F+KLD+D SG L+  D+  + S
Sbjct: 301 VGAAEFVVYKLKELGKINQEEISSFLEEFEKLDVDHSGTLSPYDLTLAQS 345

[Close]

BLAST of HG10021665.1 vs. ExPASy Swiss-Prot
Match: Q8LIN5 (Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.1e-78
Identity = 158/281 (56.23%), Postives = 195/281 (69.40%), Query Frame = 0


Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFR V +LL  YL  GT  F+L  D ++G +T   +DA+YFCVVTMTTVGYGDLVP S  
Sbjct: 64  SFRLVGLLLVAYLLLGTIAFYLAMDHMSGTRTTRALDALYFCVVTMTTVGYGDLVPASDA 123

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLACA+VF G+ + G  LSKAADY+VEKQE LL +A++      S  +++  E +K++
Sbjct: 124 AKLLACAFVFAGVAVVGTFLSKAADYLVEKQEALLFRALH------SHTMVRAMEMNKVR 183

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YK    G+LL A V  GT+ L  VE +   DAFYCVC+T+TTLGYGD SFS+  GR FAV
Sbjct: 184 YKLYTAGLLLVAAVASGTVVLWKVEGMRAVDAFYCVCATVTTLGYGDRSFSSEGGRAFAV 243

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
            WI  ST+ +A FFLY AEL+TERRQ  L  WVL R  T  DLE ADLD D  V AA+FV
Sbjct: 244 AWITVSTVVVALFFLYAAELYTERRQRELARWVLRRRTTNMDLEAADLDGDHRVGAADFV 303

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTS 342
           +YKLKE+GKI+QED+S  LD F  LD D SG L+ AD+  +
Sbjct: 304 LYKLKELGKISQEDISEFLDEFDNLDADHSGTLSPADLAAA 338

[Close]

BLAST of HG10021665.1 vs. ExPASy Swiss-Prot
Match: Q69TN4 (Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE=3 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.6e-45
Identity = 104/285 (36.49%), Postives = 169/285 (59.30%), Query Frame = 0


Query: 66  FVLLATYLGGGTFCFFLVRDQITGK--KTNGIVDAIYFCVVTMTTVGYGDLVPNSTVAKL 125
           F+ L  YL  G   +  +    T     T+ + DA+YFC+VT+ T+GYGD+ P +  AKL
Sbjct: 159 FLFLLAYLAMGVTFYAALPGNFTSSAGPTHPVADALYFCIVTLCTIGYGDITPATPAAKL 218

Query: 126 LACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAM----YMRKKISSSEILQESEAHKL 185
            + ++V  G     ++LS    Y+++ QE LL+ A+     +RK   +     +    ++
Sbjct: 219 FSISFVLIGFGFVDILLSGMVSYVLDLQEHLLITALKNPRSVRKHRHNYIFDLKKGRMRV 278

Query: 186 KYKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFA 245
           + K  +   ++   V VG   L  VE L + DA Y    ++TT+GYGD +F T AGR+FA
Sbjct: 279 RMKVALALTVVAICVGVGAAVLKRVENLGWLDAVYLAVMSVTTVGYGDHAFRTLAGRLFA 338

Query: 246 VIWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEF 305
             W++ ST+ +A+ FLYLAE+  ++R  ++ NWVLSR +T S+   AD+D++  V+ +EF
Sbjct: 339 SAWLLVSTLAVARAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGYVTKSEF 398

Query: 306 VIYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTSSSL 345
           V+YKLKEMGKI+++D+  I D F+++D    G +T +D++ S  L
Sbjct: 399 VVYKLKEMGKISEKDIMMICDQFQRMDSGNCGKITLSDLLESHQL 443

[Close]

BLAST of HG10021665.1 vs. ExPASy Swiss-Prot
Match: Q9SVV6 (Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 103/284 (36.27%), Postives = 163/284 (57.39%), Query Frame = 0


Query: 63  RKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTVAK 122
           R+ F LL  YL  G   ++L RD     +T+ +VD +YFC+VTM T+GYGD+ PNS V K
Sbjct: 151 RQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSVVTK 210

Query: 123 LLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMR---KKISSSEILQESEAHKL 182
           L +  +V  G     ++LS    Y+++ QE  ++ +   R   +K  S  I  +    ++
Sbjct: 211 LFSIMFVLVGFGFIDILLSGMVSYVLDLQESYMLDSAKRRDEPEKRRSYIIDVKKGRMRI 270

Query: 183 KYKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFA 242
           + K  +   ++   + VG   +  +E++ + D+FY    ++TT+GYGD +F T  GR+FA
Sbjct: 271 RLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGRLFA 330

Query: 243 VIWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEF 302
            IW++ ST+ +A+ FLYLAE   ++R       VL  +++ S    AD+D++  VS AE+
Sbjct: 331 AIWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSKAEY 390

Query: 303 VIYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTSSS 344
           VIYKLKEM KI  +D+ PI   F KLD   +G +T  D++   S
Sbjct: 391 VIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDLLEGGS 434

[Close]

BLAST of HG10021665.1 vs. ExPASy TrEMBL
Match: A0A0A0LW31 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G173170 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 5.0e-172
Identity = 321/342 (93.86%), Postives = 328/342 (95.91%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHV 60
           MDS D EQSLLPK SDSDK NSLLRRKSNRHGSFSHSVENNNQPQNYD VSHQRIAVS V
Sbjct: 1   MDSDDFEQSLLPKLSDSDKKNSLLRRKSNRHGSFSHSVENNNQPQNYDVVSHQRIAVSQV 60

Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNG+VD+IYFCVVTMTTVGYGDLVP+S V
Sbjct: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGVVDSIYFCVVTMTTVGYGDLVPDSMV 120

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLAC YVFTGMTLGGMILSKAADYIVEKQEILLVKAM MRKKISSSEILQESEA+KLK
Sbjct: 121 AKLLACVYVFTGMTLGGMILSKAADYIVEKQEILLVKAMCMRKKISSSEILQESEANKLK 180

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YKFIMTGILLWAL+VVG LFLTVVE LEFTDAFYCVCSTITTLGYGD+SFSTTAGRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGILFLTVVENLEFTDAFYCVCSTITTLGYGDQSFSTTAGRVFAV 240

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
           IWIMS TICLAQFFLYLAEL+TERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSGTICLAQFFLYLAELYTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTSS 343
           IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIV  S
Sbjct: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVVIS 342

[Close]

BLAST of HG10021665.1 vs. ExPASy TrEMBL
Match: A0A1S4E4K8 (LOW QUALITY PROTEIN: two-pore potassium channel 1-like OS=Cucumis melo OX=3656 GN=LOC103502205 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.7e-167
Identity = 314/341 (92.08%), Postives = 326/341 (95.60%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHV 60
           MDSGDVEQSLL K  DSDK NSLLRRKSNRHGSFSHSVE NN+PQNYD V+HQR+AVS +
Sbjct: 1   MDSGDVEQSLLLKLFDSDKRNSLLRRKSNRHGSFSHSVE-NNKPQNYD-VAHQRVAVSQI 60

Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFRKVFVLLATYLGGGTFCF LVRDQITGKKTNG+VD+IYFCVVTMTTVGYGDLVPNS V
Sbjct: 61  SFRKVFVLLATYLGGGTFCFXLVRDQITGKKTNGVVDSIYFCVVTMTTVGYGDLVPNSMV 120

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLAC YVFTGMTLGGMILSKAADYIVEKQEILLVKAM MRKKISSSEILQESE +KLK
Sbjct: 121 AKLLACVYVFTGMTLGGMILSKAADYIVEKQEILLVKAMRMRKKISSSEILQESEENKLK 180

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YKFIMTGILLWAL+VVGTLFLTVVE LEFTDAFYCVCSTITTLGYGD+SFSTTAGRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGTLFLTVVENLEFTDAFYCVCSTITTLGYGDQSFSTTAGRVFAV 240

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
           IWIMS TICLAQFFLYLAELHTERRQ+SL+NWVL+RSLTYSDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSGTICLAQFFLYLAELHTERRQDSLINWVLNRSLTYSDLEEADLDHDKVVSAAEFV 300

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTS 342
           IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIV S
Sbjct: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVIS 339

[Close]

BLAST of HG10021665.1 vs. ExPASy TrEMBL
Match: A0A6J1CMK6 (two-pore potassium channel 1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012651 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.9e-155
Identity = 292/341 (85.63%), Postives = 311/341 (91.20%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHV 60
           MD GDVEQSLLP  +DS K N+L RRKS R GSFS  VENNNQPQ  D VSHQ      +
Sbjct: 1   MDLGDVEQSLLPNLADSGKRNALQRRKSARRGSFSQLVENNNQPQICDEVSHQSTIAQQI 60

Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFR+VFVLLATYLGGGT CFFLV+DQI+GKKTNGIVDA+YFCVVTMTTVGYGDLVP+S V
Sbjct: 61  SFRQVFVLLATYLGGGTLCFFLVQDQISGKKTNGIVDALYFCVVTMTTVGYGDLVPSSLV 120

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVK MYMR  ISSSEI+QESEAHKLK
Sbjct: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKTMYMRNNISSSEIVQESEAHKLK 180

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YKFIMTGILLWAL+VVGTLFLT VEKLEF DAFYCVCSTITTLGYGDESFST+AGRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGTLFLTAVEKLEFMDAFYCVCSTITTLGYGDESFSTSAGRVFAV 240

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
           IWI+SSTICLAQFFLYLAEL TERRQ+SLVNWVL+R+LT+ DLEEADLDHDKVVSAAEFV
Sbjct: 241 IWILSSTICLAQFFLYLAELRTERRQKSLVNWVLNRNLTHLDLEEADLDHDKVVSAAEFV 300

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTS 342
           +YKLKEMGKI QED+SPILDTFKKLDIDQSG LTEAD+VTS
Sbjct: 301 LYKLKEMGKICQEDISPILDTFKKLDIDQSGSLTEADLVTS 341

[Close]

BLAST of HG10021665.1 vs. ExPASy TrEMBL
Match: A0A6J1CLA7 (two-pore potassium channel 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012651 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.5e-155
Identity = 292/341 (85.63%), Postives = 311/341 (91.20%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHV 60
           MD GDVEQSLLP  +DS K N+L RRKS R GSFS  VENNNQPQ  D VSHQ      +
Sbjct: 1   MDLGDVEQSLLPNLADSGKRNALQRRKSARSGSFSQLVENNNQPQICDEVSHQSTIAQQI 60

Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFR+VFVLLATYLGGGT CFFLV+DQI+GKKTNGIVDA+YFCVVTMTTVGYGDLVP+S V
Sbjct: 61  SFRQVFVLLATYLGGGTLCFFLVQDQISGKKTNGIVDALYFCVVTMTTVGYGDLVPSSLV 120

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVK MYMR  ISSSEI+QESEAHKLK
Sbjct: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKTMYMRNNISSSEIVQESEAHKLK 180

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YKFIMTGILLWAL+VVGTLFLT VEKLEF DAFYCVCSTITTLGYGDESFST+AGRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGTLFLTAVEKLEFMDAFYCVCSTITTLGYGDESFSTSAGRVFAV 240

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
           IWI+SSTICLAQFFLYLAEL TERRQ+SLVNWVL+R+LT+ DLEEADLDHDKVVSAAEFV
Sbjct: 241 IWILSSTICLAQFFLYLAELRTERRQKSLVNWVLNRNLTHLDLEEADLDHDKVVSAAEFV 300

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTS 342
           +YKLKEMGKI QED+SPILDTFKKLDIDQSG LTEAD+VTS
Sbjct: 301 LYKLKEMGKICQEDISPILDTFKKLDIDQSGSLTEADLVTS 341

[Close]

BLAST of HG10021665.1 vs. ExPASy TrEMBL
Match: A0A6J1HTF8 (two-pore potassium channel 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466596 PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.9e-151
Identity = 286/341 (83.87%), Postives = 308/341 (90.32%), Query Frame = 0


Query: 1   MDSGDVEQSLLPKPSDSDKMNSLLRRKSNRHGSFSHSVENNNQPQNYDAVSHQRIAVSHV 60
           M+SGDVEQSLLPK SDS+K NSL RRKS  H  FS S+E+N+QP+N D  SHQR+ V  V
Sbjct: 1   MESGDVEQSLLPKLSDSNKRNSLQRRKSIHHEIFSRSLEHNDQPRNGDEASHQRVVVKQV 60

Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           SFRKVFV+LATYLGGGT CFFLV DQI GKKTNGIVDAIYFCVVTMTTVGYGDLVPNS  
Sbjct: 61  SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 120

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           AKLLACAYVFTG+TLGGM+LS+AADYIVEKQEILLVKA++M KKISS E+L+ES AHKLK
Sbjct: 121 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEILLVKAIFMSKKISSGELLRESVAHKLK 180

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YK +MTGI+LW LVVVGTLFL +VEK EFTDAFYCVCSTITTLGYGD+SFSTT GRVFAV
Sbjct: 181 YKILMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 240

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
           IWIMSSTICLAQFFLYLAEL  ERRQ+SLVN VLSR LTYSDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTYSDLEEADLDHDKVVSAAEFV 300

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTS 342
           +YKLKEMGKINQEDVSPILDTF KLDIDQSG LTEAD+VTS
Sbjct: 301 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 341

[Close]

BLAST of HG10021665.1 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 352.8 bits (904), Expect = 3.1e-97
Identity = 172/283 (60.78%), Postives = 232/283 (81.98%), Query Frame = 0


Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           + R+V + LA YL  GT CF+LVRDQI+G KT+G+VDA+YFC+VTMTTVGYGDLVPNS+ 
Sbjct: 75  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 134

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           ++LLACA+VF+GM L G +LS+AADY+VEKQE LLV+A ++R+    ++IL+E   +KL+
Sbjct: 135 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLR 194

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YK   T ++L  L +VGT+FL +VEK+    AFYCVCST+TTLGYGD+SF++ AGR+FAV
Sbjct: 195 YKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAV 254

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
            WI++S+ICLAQFFLY+AEL+TE +Q +LV WVL+R +T +DLE ADLD D VV AAEF+
Sbjct: 255 FWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFI 314

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTSSS 344
           +YKLKEMGKI+++D+S I+D F++LD D+SG LT +DIV + +
Sbjct: 315 VYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQT 357

[Close]

BLAST of HG10021665.1 vs. TAIR 10
Match: AT5G55630.2 (Outward rectifying potassium channel protein )

HSP 1 Score: 352.8 bits (904), Expect = 3.1e-97
Identity = 172/283 (60.78%), Postives = 232/283 (81.98%), Query Frame = 0


Query: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTV 120
           + R+V + LA YL  GT CF+LVRDQI+G KT+G+VDA+YFC+VTMTTVGYGDLVPNS+ 
Sbjct: 75  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 134

Query: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRKKISSSEILQESEAHKLK 180
           ++LLACA+VF+GM L G +LS+AADY+VEKQE LLV+A ++R+    ++IL+E   +KL+
Sbjct: 135 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLR 194

Query: 181 YKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAV 240
           YK   T ++L  L +VGT+FL +VEK+    AFYCVCST+TTLGYGD+SF++ AGR+FAV
Sbjct: 195 YKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAV 254

Query: 241 IWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300
            WI++S+ICLAQFFLY+AEL+TE +Q +LV WVL+R +T +DLE ADLD D VV AAEF+
Sbjct: 255 FWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFI 314

Query: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTSSS 344
           +YKLKEMGKI+++D+S I+D F++LD D+SG LT +DIV + +
Sbjct: 315 VYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQT 357

[Close]

BLAST of HG10021665.1 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 181.8 bits (460), Expect = 9.4e-46
Identity = 103/284 (36.27%), Postives = 163/284 (57.39%), Query Frame = 0


Query: 63  RKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSTVAK 122
           R+ F LL  YL  G   ++L RD     +T+ +VD +YFC+VTM T+GYGD+ PNS V K
Sbjct: 151 RQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSVVTK 210

Query: 123 LLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMR---KKISSSEILQESEAHKL 182
           L +  +V  G     ++LS    Y+++ QE  ++ +   R   +K  S  I  +    ++
Sbjct: 211 LFSIMFVLVGFGFIDILLSGMVSYVLDLQESYMLDSAKRRDEPEKRRSYIIDVKKGRMRI 270

Query: 183 KYKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFA 242
           + K  +   ++   + VG   +  +E++ + D+FY    ++TT+GYGD +F T  GR+FA
Sbjct: 271 RLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGRLFA 330

Query: 243 VIWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEF 302
            IW++ ST+ +A+ FLYLAE   ++R       VL  +++ S    AD+D++  VS AE+
Sbjct: 331 AIWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSKAEY 390

Query: 303 VIYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIVTSSS 344
           VIYKLKEM KI  +D+ PI   F KLD   +G +T  D++   S
Sbjct: 391 VIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDLLEGGS 434

[Close]

BLAST of HG10021665.1 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 178.3 bits (451), Expect = 1.0e-44
Identity = 112/318 (35.22%), Postives = 182/318 (57.23%), Query Frame = 0


Query: 36  HSVENNNQPQNYDAVSHQRIAVSHVSFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGI 95
           +++ + N P+  D  S  +  V+     +   LL  YL  G   ++L RD    K+T+ +
Sbjct: 126 NNISHPNDPKT-DQQSDSKTIVN-----QAVALLVVYLSLGVLIYWLNRDSYNVKQTHPV 185

Query: 96  VDAIYFCVVTMTTVGYGDLVPNSTVAKLLACAYVFTGMTLGGMILSKAADYIVEKQEILL 155
           VDA+YFC+VTM T+GYGD+ P+S V KL +  +V  G     ++LS    Y+++ QE  +
Sbjct: 186 VDALYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYM 245

Query: 156 VKA-------MYMRKKISSSEILQESEAHKLKYKFIMTGILLWALVV---VGTLFLTVVE 215
           ++        +  R K+ S  I  +    +++ K    G+ L  +V+    G L +  VE
Sbjct: 246 LETARNESLNLNDRDKVRSYIIDVKKGRMRIRLK---VGLALGVVVLCLGFGVLIMHFVE 305

Query: 216 KLEFTDAFYCVCSTITTLGYGDESFSTTAGRVFAVIWIMSSTICLAQFFLYLAELHTERR 275
           K+ + D+FY    ++TT+GYGD +F+T AGR+ A +W++ ST+ +A+  L+LAE   ++R
Sbjct: 306 KIGWLDSFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKR 365

Query: 276 QESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFVIYKLKEMGKINQEDVSPILDTFKKL 335
                  VL  S++ S   +AD+D +  VS AEFVIYKLK+M KI ++D++PI   F KL
Sbjct: 366 NRERAKKVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKL 425

Query: 336 DIDQSGCLTEADIVTSSS 344
           D   SG +T  D++ SS+
Sbjct: 426 DRTNSGRITLLDLLESST 434

[Close]

BLAST of HG10021665.1 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 176.4 bits (446), Expect = 3.9e-44
Identity = 114/350 (32.57%), Postives = 192/350 (54.86%), Query Frame = 0


Query: 5   DVEQSLL----PKPSDSDKMNSLLRRKSNRHGS-FSHSVENNNQPQNYDAVSHQRIAVSH 64
           D+EQS+      + SDSD+ N  L +    H S  + ++      +     + +   VS 
Sbjct: 55  DLEQSVQDDKEDQDSDSDETNRFLSQTRPLHRSRTAPAMVIIKDLRTKPPETKKPSPVSK 114

Query: 65  VSFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGIVDAIYFCVVTMTTVGYGDLVPNST 124
              R+   LL  YL  G   +   RD  +G +T+ +VDA+YFC+VTM T+GYGD+ P + 
Sbjct: 115 SIIRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTP 174

Query: 125 VAKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKAMYMRK--------KISSSEIL 184
             K+ A  +V  G     ++LS   +Y+++ QE +++  +  R+        + S+ + +
Sbjct: 175 WTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYI 234

Query: 185 QESE--AHKLKYKFIMTGILLWALVVVGTLFLTVVEKLEFTDAFYCVCSTITTLGYGDES 244
            + E    +++ K  +   ++   + VG L L  VE+L F D+ Y    ++TT+GYGD +
Sbjct: 235 IDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRA 294

Query: 245 FSTTAGRVFAVIWIMSSTICLAQFFLYLAELHTERRQESLVNWVLSRSLTYSDLEEADLD 304
           F T  GR+FA +W++ ST+ +A+ FLYLAE   +RR    V   L+R +T  DL +AD  
Sbjct: 295 FKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTY 354

Query: 305 HDKVVSAAEFVIYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIV 340
               +S +E+++ KLKEMGKI Q+D+  ++  F+KLD +Q G +T  D++
Sbjct: 355 QHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDLL 404

The following BLAST results are available for this feature:

BLAST of HG10021665.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877797.1	6.1e-172	94.72	two-pore potassium channel 1-like [Benincasa hispida]	[more]
XP_004139602.1	1.0e-171	93.86	two-pore potassium channel 1 [Cucumis sativus] >KGN64997.1 hypothetical protein ...	[more]
XP_016903168.1	7.7e-167	92.08	PREDICTED: LOW QUALITY PROTEIN: two-pore potassium channel 1-like [Cucumis melo]	[more]
XP_022142571.1	4.0e-155	85.63	two-pore potassium channel 1-like isoform X2 [Momordica charantia]	[more]
XP_022142569.1	5.2e-155	85.63	two-pore potassium channel 1-like isoform X1 [Momordica charantia] >XP_022142570...	[more]

BLAST of HG10021665.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LBL1	4.3e-96	60.78	Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2	[more]
Q850M0	1.3e-87	50.57	Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE...	[more]
Q8LIN5	3.1e-78	56.23	Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE...	[more]
Q69TN4	1.6e-45	36.49	Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE...	[more]
Q9SVV6	1.3e-44	36.27	Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1	[more]

BLAST of HG10021665.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LW31	5.0e-172	93.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G173170 PE=3 SV=1	[more]
A0A1S4E4K8	3.7e-167	92.08	LOW QUALITY PROTEIN: two-pore potassium channel 1-like OS=Cucumis melo OX=3656 G...	[more]
A0A6J1CMK6	1.9e-155	85.63	two-pore potassium channel 1-like isoform X2 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1CLA7	2.5e-155	85.63	two-pore potassium channel 1-like isoform X1 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1HTF8	2.9e-151	83.87	two-pore potassium channel 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of HG10021665.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55630.1	3.1e-97	60.78	Outward rectifying potassium channel protein	[more]
AT5G55630.2	3.1e-97	60.78	Outward rectifying potassium channel protein	[more]
AT4G18160.1	9.4e-46	36.27	Ca2+ activated outward rectifying K+ channel 6	[more]
AT5G46370.1	1.0e-44	35.22	Ca2+ activated outward rectifying K+ channel 2	[more]
AT4G01840.1	3.9e-44	32.57	Ca2+ activated outward rectifying K+ channel 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003280	Two pore domain potassium channel	PRINTS	PR01333	2POREKCHANEL	coord: 221..230 score: 45.77 coord: 104..132 score: 31.83
IPR003280	Two pore domain potassium channel	PANTHER	PTHR11003	POTASSIUM CHANNEL, SUBFAMILY K	coord: 20..339
None	No IPR available	GENE3D	1.10.238.10		coord: 282..343 e-value: 1.8E-5 score: 26.2
None	No IPR available	GENE3D	1.10.287.70		coord: 157..273 e-value: 2.8E-16 score: 61.1
None	No IPR available	GENE3D	1.10.287.70		coord: 56..153 e-value: 1.6E-20 score: 74.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR11003:SF271	OUTWARD RECTIFYING POTASSIUM CHANNEL PROTEIN	coord: 20..339
None	No IPR available	SUPERFAMILY	81324	Voltage-gated potassium channels	coord: 58..162
None	No IPR available	SUPERFAMILY	81324	Voltage-gated potassium channels	coord: 178..276
IPR013099	Potassium channel domain	PFAM	PF07885	Ion_trans_2	coord: 190..259 e-value: 4.9E-12 score: 45.6 coord: 69..148 e-value: 1.6E-15 score: 56.8
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 326..338
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 272..339

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10021665	HG10021665	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10021665.1-cds	HG10021665.1-cds-Chr05:13466505..13466658	CDS
HG10021665.1-cds	HG10021665.1-cds-Chr05:13466804..13467687	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10021665.1	HG10021665.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071805	potassium ion transmembrane transport
biological_process	GO:0030322	stabilization of membrane potential
cellular_component	GO:0016020	membrane
cellular_component	GO:0005887	integral component of plasma membrane
cellular_component	GO:0009705	plant-type vacuole membrane
molecular_function	GO:0005267	potassium channel activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0022841	potassium ion leak channel activity