HG10020354.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10020354.1 vs. NCBI nr
Match: XP_038903919.1 (protein NRT1/ PTR FAMILY 5.6-like [Benincasa hispida])

HSP 1 Score: 1059.7 bits (2739), Expect = 9.4e-306
Identity = 543/595 (91.26%), Postives = 566/595 (95.13%), Query Frame = 0

Query: 1   MKTD-QLRKERELLGDEDEPK-NKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIE 60
           MK D QL  +R+L   ED+PK NKDNQ WVYDSSVDHKGNLPLRASTGVWKSSLFIIAIE
Sbjct: 1   MKPDHQLGNKRKLF--EDDPKLNKDNQTWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIE 60

Query: 61  FSERLSYFGIATSLVIYLTRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFST 120
           FSERLSYFGIATSLVIYLTRV+HEDLKTAARNVNYWTGVTTLMPLLGGFLADAY GRFST
Sbjct: 61  FSERLSYFGIATSLVIYLTRVLHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYFGRFST 120

Query: 121 VLLSTIIYLLGLSLLTMSTMVPSLKPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPS 180
           VL+STIIYLLGLSLLTMST+VPSLKPCGSETCEEPRK+HEILFFTAIYLISIGTGGHKPS
Sbjct: 121 VLISTIIYLLGLSLLTMSTLVPSLKPCGSETCEEPRKLHEILFFTAIYLISIGTGGHKPS 180

Query: 181 LESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTS 240
           LESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQ+ VGWGMAGVIL+S
Sbjct: 181 LESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQDRVGWGMAGVILSS 240

Query: 241 VMALSLAIFLLGRSVYRFRAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKF 300
           VMA+SLAIFLLGR VYRFRAPLGSPLTPLLQVIVAAFR RNL YPPHSSHLYE+Q++DKF
Sbjct: 241 VMAISLAIFLLGRPVYRFRAPLGSPLTPLLQVIVAAFRKRNLDYPPHSSHLYEVQSSDKF 300

Query: 301 QGRLLSHTKNLKFLDKAAIIEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLP 360
           QGRLL HTKNL FLDKAAIIE+ + NSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLP
Sbjct: 301 QGRLLFHTKNLTFLDKAAIIED-TDNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLP 360

Query: 361 FGICVAQASTFFVKQSGTLDRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKT 420
           F ICVAQASTFFVKQ GTLDRK+GN FI+PASSMYC AAAGMILSVAIYDK+LVPLLRKT
Sbjct: 361 FSICVAQASTFFVKQCGTLDRKIGNKFIIPASSMYCSAAAGMILSVAIYDKLLVPLLRKT 420

Query: 421 TGNERGISILQRIGIGMIFSFTTMVVAALVERKRLSQTADA--MSVFWLAPQFFIIGIGD 480
           TGNERGISILQRIGIGMIFSFTTMVVAALVERKRL +TA+A  MSVFWLAPQFFIIGIGD
Sbjct: 421 TGNERGISILQRIGIGMIFSFTTMVVAALVERKRLIRTAEAMSMSVFWLAPQFFIIGIGD 480

Query: 481 GFALVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGE 540
           GFALVGLQEYFYDQVPDSMRSLGIAFYLS+NGAANFLSSLLIT+ DRITKKSSGKSWFGE
Sbjct: 481 GFALVGLQEYFYDQVPDSMRSLGIAFYLSINGAANFLSSLLITIADRITKKSSGKSWFGE 540

Query: 541 DLNSSRLDNFYWLIAGIVAVDLCVYVLLARRYTYKSVQKTTVADCYDGKGPHATS 592
           DLNSSRLDNFYWLIA IVAVDLCVYV LARRYTYKSVQKTTVADCYDGKGP A+S
Sbjct: 541 DLNSSRLDNFYWLIAAIVAVDLCVYVFLARRYTYKSVQKTTVADCYDGKGPDASS 592

BLAST of HG10020354.1 vs. NCBI nr
Match: KAA0044126.1 (protein NRT1/ PTR FAMILY 5.6 [Cucumis melo var. makuwa])

HSP 1 Score: 1036.2 bits (2678), Expect = 1.1e-298
Identity = 516/577 (89.43%), Postives = 546/577 (94.63%), Query Frame = 0

Query: 17  DEPKNKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYL 76
           D+   +D+QIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYL
Sbjct: 4   DQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYL 63

Query: 77  TRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMS 136
           TRV+HEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVL+ST+IYLLGLSLLTMS
Sbjct: 64  TRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLISTVIYLLGLSLLTMS 123

Query: 137 TMVPSLKPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERK 196
           T+VPSLKPC  ETC+EPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERK
Sbjct: 124 TLVPSLKPCDGETCQEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERK 183

Query: 197 QKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRF 256
           QKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGM GVILTSVMA+SLAIFLLGR VYR+
Sbjct: 184 QKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRY 243

Query: 257 RAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQGRLLSHTKNLKFLDKAA 316
           RAP GSPLTPLLQVI+AAFR R L YPPHSS L+E+Q+ DKFQGRLLSHTKNL+FLDKAA
Sbjct: 244 RAPSGSPLTPLLQVIIAAFRKRKLDYPPHSSQLHEVQSGDKFQGRLLSHTKNLRFLDKAA 303

Query: 317 IIEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGT 376
           I+EER GNS+ K+GAWRLATVTRVEELKLVLNMIPIWITSLPF ICVAQASTFF+KQ GT
Sbjct: 304 IVEER-GNSKEKQGAWRLATVTRVEELKLVLNMIPIWITSLPFSICVAQASTFFIKQCGT 363

Query: 377 LDRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMI 436
           LDRK+GN F++P SSMYCLAAAGMI+SVAIYDK+LVPLLRKTTGNERGISILQRIGIGM+
Sbjct: 364 LDRKIGNTFVIPVSSMYCLAAAGMIISVAIYDKLLVPLLRKTTGNERGISILQRIGIGMV 423

Query: 437 FSFTTMVVAALVERKRLSQTADAMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMR 496
           FSFTTMVV+ALVERKRLS T   MSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMR
Sbjct: 424 FSFTTMVVSALVERKRLSHTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMR 483

Query: 497 SLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNSSRLDNFYWLIAGIVAV 556
           SLGIAFYLSVNGAANFLSS +IT+VDRITKKSSGKSWFG+DLNSSRLDNFYWLIAGIVAV
Sbjct: 484 SLGIAFYLSVNGAANFLSSFIITIVDRITKKSSGKSWFGDDLNSSRLDNFYWLIAGIVAV 543

Query: 557 DLCVYVLLARRYTYKSVQKTTVADCYDGKGPHATSPV 594
           DLCVYV LA RYTYKSVQKT V DCYD KGP+A S V
Sbjct: 544 DLCVYVFLAHRYTYKSVQKTAVGDCYDDKGPNADSAV 579

BLAST of HG10020354.1 vs. NCBI nr
Match: XP_008442499.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.6 [Cucumis melo])

HSP 1 Score: 1035.0 bits (2675), Expect = 2.5e-298
Identity = 522/594 (87.88%), Postives = 554/594 (93.27%), Query Frame = 0

Query: 1   MKT-DQLRKERELLGDEDEPKNKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEF 60
           MKT +QL K +      D+   +D+QIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEF
Sbjct: 1   MKTNNQLEKMK-----RDQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEF 60

Query: 61  SERLSYFGIATSLVIYLTRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTV 120
           SERLSYFGIATSLVIYLTRV+HEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTV
Sbjct: 61  SERLSYFGIATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTV 120

Query: 121 LLSTIIYLLGLSLLTMSTMVPSLKPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSL 180
           L+ST+IYLLGLSLLTMST+VPSLKPC  ETC+EPRKVHEILFFTAIYLISIGTGGHKPSL
Sbjct: 121 LISTVIYLLGLSLLTMSTLVPSLKPCDGETCQEPRKVHEILFFTAIYLISIGTGGHKPSL 180

Query: 181 ESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSV 240
           ESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGM GVILTSV
Sbjct: 181 ESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSV 240

Query: 241 MALSLAIFLLGRSVYRFRAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQ 300
           MA+SLAIFLLGR VYR+RAP GSPLTPLLQVI+AAFR R L YPPHSS L+E+Q+ DKFQ
Sbjct: 241 MAISLAIFLLGRPVYRYRAPSGSPLTPLLQVIIAAFRKRKLDYPPHSSQLHEVQSGDKFQ 300

Query: 301 GRLLSHTKNLKFLDKAAIIEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPF 360
           GRLLSHTKNL+FLDKAAI+EER GNS+ K+GAWRLATVTRVEELKLVLNMIPIWITSLPF
Sbjct: 301 GRLLSHTKNLRFLDKAAIVEER-GNSKEKQGAWRLATVTRVEELKLVLNMIPIWITSLPF 360

Query: 361 GICVAQASTFFVKQSGTLDRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTT 420
            ICVAQASTFF+KQ GTLDRK+GN F++P SSMYCLAAAGMI+SVAIYDK+LVPLLRKTT
Sbjct: 361 SICVAQASTFFIKQCGTLDRKIGNTFVIPVSSMYCLAAAGMIISVAIYDKLLVPLLRKTT 420

Query: 421 GNERGISILQRIGIGMIFSFTTMVVAALVERKRLSQTADAMSVFWLAPQFFIIGIGDGFA 480
           GNERGISILQRIGIGM+FSFTTMVV+ALVERKRLS T   MSVFWLAPQFFIIGIGDGFA
Sbjct: 421 GNERGISILQRIGIGMVFSFTTMVVSALVERKRLSHTTHPMSVFWLAPQFFIIGIGDGFA 480

Query: 481 LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLN 540
           LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSS +IT+VDRITKKSSGKSWFG+DLN
Sbjct: 481 LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFIITIVDRITKKSSGKSWFGDDLN 540

Query: 541 SSRLDNFYWLIAGIVAVDLCVYVLLARRYTYKSVQKTTVADCYDGKGPHATSPV 594
           SSRLDNFYWLIAGIVAVDLCVYV LA RYTYKSVQKT V DCYD KGP+A S V
Sbjct: 541 SSRLDNFYWLIAGIVAVDLCVYVFLAHRYTYKSVQKTAVGDCYDDKGPNADSAV 588

BLAST of HG10020354.1 vs. NCBI nr
Match: XP_031737503.1 (protein NRT1/ PTR FAMILY 5.6 [Cucumis sativus] >KAE8650995.1 hypothetical protein Csa_001365 [Cucumis sativus])

HSP 1 Score: 1029.2 bits (2660), Expect = 1.4e-296
Identity = 520/594 (87.54%), Postives = 555/594 (93.43%), Query Frame = 0

Query: 1   MKT-DQLRKERELLGDEDEPKNKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEF 60
           MKT +QL K +      D+   +D+QIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEF
Sbjct: 1   MKTNNQLEKIK-----RDQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEF 60

Query: 61  SERLSYFGIATSLVIYLTRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTV 120
           SERLSYFGIATSLVIYLT+V+HEDLKTAARNVNYWTGVTTLMPL GGFLADAYLGRFSTV
Sbjct: 61  SERLSYFGIATSLVIYLTKVIHEDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTV 120

Query: 121 LLSTIIYLLGLSLLTMSTMVPSLKPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSL 180
           L+ST+IYLLGLSLLT+ST+VPSLKPCG ETCEEPRKVHEILFFTAIYLISIGTGGHKPSL
Sbjct: 121 LISTVIYLLGLSLLTLSTLVPSLKPCGGETCEEPRKVHEILFFTAIYLISIGTGGHKPSL 180

Query: 181 ESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSV 240
           ESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGM GVILTSV
Sbjct: 181 ESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSV 240

Query: 241 MALSLAIFLLGRSVYRFRAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQ 300
           MA+SLAIFLLGR VYRFRAPLGSPLTPLLQVIVAAFRNR L YPP+SS L+E+Q+ DKFQ
Sbjct: 241 MAISLAIFLLGRPVYRFRAPLGSPLTPLLQVIVAAFRNRKLGYPPNSSQLHEVQSGDKFQ 300

Query: 301 GRLLSHTKNLKFLDKAAIIEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPF 360
           GRLLSHTKNLKFLDKAAI++ER  NSE K+GAWRL+TVTRVEELKLVLNMIPIWITSLPF
Sbjct: 301 GRLLSHTKNLKFLDKAAIVDERD-NSEEKQGAWRLSTVTRVEELKLVLNMIPIWITSLPF 360

Query: 361 GICVAQASTFFVKQSGTLDRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTT 420
           GICVAQASTFFVKQ GTLDRK+GNNF++PASSM+CL+A GMI+SVAIYDK+LVPLLRK T
Sbjct: 361 GICVAQASTFFVKQCGTLDRKIGNNFVIPASSMFCLSAIGMIISVAIYDKLLVPLLRKIT 420

Query: 421 GNERGISILQRIGIGMIFSFTTMVVAALVERKRLSQTADAMSVFWLAPQFFIIGIGDGFA 480
           GNERGISILQRIGIGM+FSFTTMVV+ALVERKRL  T   M+VFWLAPQFFIIGIGDGFA
Sbjct: 421 GNERGISILQRIGIGMVFSFTTMVVSALVERKRLDHTTHPMNVFWLAPQFFIIGIGDGFA 480

Query: 481 LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLN 540
           LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANF SS LIT+VD+ITKKSSGKSWFG+DLN
Sbjct: 481 LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFFSSFLITIVDQITKKSSGKSWFGDDLN 540

Query: 541 SSRLDNFYWLIAGIVAVDLCVYVLLARRYTYKSVQKTTVADCYDGKGPHATSPV 594
           SSRLDNFYWLIAGIVAVDLCVYV LARRYTYKSVQKTTVADC+D KG   +S V
Sbjct: 541 SSRLDNFYWLIAGIVAVDLCVYVFLARRYTYKSVQKTTVADCHDVKGRDVSSAV 588

BLAST of HG10020354.1 vs. NCBI nr
Match: XP_022145543.1 (protein NRT1/ PTR FAMILY 5.6-like isoform X1 [Momordica charantia])

HSP 1 Score: 963.0 bits (2488), Expect = 1.2e-276
Identity = 485/596 (81.38%), Postives = 535/596 (89.77%), Query Frame = 0

Query: 6   LRKERELLGDEDEPK-------NKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIE 65
           ++ ERE   D+ + K       N  N   V+DSSVDHKG +PLRASTGVWK+SLFIIAIE
Sbjct: 1   MKTEREEFTDQPKNKIHHNNNNNNHNHDLVHDSSVDHKGRIPLRASTGVWKASLFIIAIE 60

Query: 66  FSERLSYFGIATSLVIYLTRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFST 125
           FSERLSYFGIATSLVIYLT+V+ +DLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFST
Sbjct: 61  FSERLSYFGIATSLVIYLTKVIRQDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFST 120

Query: 126 VLLSTIIYLLGLSLLTMSTMVPSLKPCGSE-TCEEPRKVHEILFFTAIYLISIGTGGHKP 185
           VL STI+YLLGLSLLT+ST+VPSLK CG+E TC++PRK+HE+LFFTAIY IS+GTGGHKP
Sbjct: 121 VLASTIVYLLGLSLLTVSTLVPSLKACGAEITCDQPRKIHEVLFFTAIYFISVGTGGHKP 180

Query: 186 SLESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILT 245
           SLESFGADQFDDDH EERKQKMSFFNWWNSGLCAGVI GVTLIVYVQ+HVGWG++G ILT
Sbjct: 181 SLESFGADQFDDDHAEERKQKMSFFNWWNSGLCAGVILGVTLIVYVQDHVGWGVSGAILT 240

Query: 246 SVMALSLAIFLLGRSVYRFRAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDK 305
           SVMA+SLAIFLLGR VYRFRAPLGSPLTPLLQV+VAAFRNRNL YPPH SHLYELQ+T  
Sbjct: 241 SVMAISLAIFLLGRPVYRFRAPLGSPLTPLLQVLVAAFRNRNLPYPPHPSHLYELQSTHN 300

Query: 306 FQGRLLSHTKNLKFLDKAAIIEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSL 365
            QGRLLSHTK LKFLDKAAIIEE +GNS GK+ AWRLATVTRVEELKL+LNMIPIWITSL
Sbjct: 301 IQGRLLSHTKKLKFLDKAAIIEE-TGNSSGKQSAWRLATVTRVEELKLILNMIPIWITSL 360

Query: 366 PFGICVAQASTFFVKQSGTLDRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRK 425
           PFGICVAQ STFF+KQ  TLDRK+GN+FI+PASSM+CLAAAGMI+SVAIYDK++VP+LRK
Sbjct: 361 PFGICVAQTSTFFIKQCLTLDRKIGNSFIMPASSMFCLAAAGMIVSVAIYDKVIVPILRK 420

Query: 426 TTGNERGISILQRIGIGMIFSFTTMVVAALVERKRLSQTAD-------AMSVFWLAPQFF 485
           TTGNERGISILQRIGIGM+FS T+M VAA+VERKRL    D        MSVFWLAPQF 
Sbjct: 421 TTGNERGISILQRIGIGMVFSLTSMAVAAVVERKRLDVVKDNPTKGSMEMSVFWLAPQFL 480

Query: 486 IIGIGDGFALVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSG 545
           IIGI DGFALVGLQEYFYDQVPDSMRS+GIA YLSVNGAANF+SSLLIT+VDRITKKS+ 
Sbjct: 481 IIGIADGFALVGLQEYFYDQVPDSMRSIGIALYLSVNGAANFVSSLLITVVDRITKKSND 540

Query: 546 KSWFGEDLNSSRLDNFYWLIAGIVAVDLCVYVLLARRYTYKSVQKTTVADCYDGKG 587
           KSWFG+DLNSSRLDNFYWLIA +VAV+LCVYV LARRY+YK++QKTTVADCYDG G
Sbjct: 541 KSWFGDDLNSSRLDNFYWLIAAVVAVNLCVYVFLARRYSYKTLQKTTVADCYDGDG 595

BLAST of HG10020354.1 vs. ExPASy Swiss-Prot
Match: P0CI03 (Protein NRT1/ PTR FAMILY 5.6 OS=Arabidopsis thaliana OX=3702 GN=NPF5.6 PE=2 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 3.0e-214
Identity = 360/554 (64.98%), Postives = 449/554 (81.05%), Query Frame = 0

Query: 23  DNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVMHE 82
           D Q WV DSS+D +G +PLRA TG W+++LFIIAIEFSERLSYFG+AT+LV+YLT ++++
Sbjct: 17  DQQKWVLDSSLDSRGRVPLRARTGAWRAALFIIAIEFSERLSYFGLATNLVVYLTTILNQ 76

Query: 83  DLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTMVPSL 142
           DLK A RNVNYW+GVTTLMPLLGGF+ADAYLGR++TVL++T IYL+GL LLTMS  +P L
Sbjct: 77  DLKMAIRNVNYWSGVTTLMPLLGGFIADAYLGRYATVLVATTIYLMGLVLLTMSWFIPGL 136

Query: 143 KPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERKQKMSFF 202
           KPC  E C EPRK HE+ FF AIYLISIGTGGHKPSLESFGADQFDDDH EERK KMSFF
Sbjct: 137 KPCHQEVCVEPRKAHEVAFFIAIYLISIGTGGHKPSLESFGADQFDDDHVEERKMKMSFF 196

Query: 203 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRFRAPLGS 262
           NWWN  LCAG++  VT + Y+++ VGWG+AG+ILT VMA+SL IF +G+  YR+R P GS
Sbjct: 197 NWWNVSLCAGILTAVTAVAYIEDRVGWGVAGIILTVVMAISLIIFFIGKPFYRYRTPSGS 256

Query: 263 PLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQGRLLSHTKNLKFLDKAAIIEERS 322
           PLTP+LQV VAA   RNL YP   S L+E+  T+   GRLL HT++LKFLDKAAIIE+++
Sbjct: 257 PLTPILQVFVAAIAKRNLPYPSDPSLLHEVSKTEFTSGRLLCHTEHLKFLDKAAIIEDKN 316

Query: 323 GNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGTLDRKLG 382
             +  K+  WRL T+T+VEE KL++N+IPIW ++L FGIC  QASTFF+KQ+ T+DR +G
Sbjct: 317 PLALEKQSPWRLLTLTKVEETKLIINVIPIWFSTLAFGICATQASTFFIKQAITMDRHIG 376

Query: 383 NNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMIFSFTTM 442
             F +P +SM+ L A  +I+S+ +Y+K+LVPLLR  T N+RGI+ILQRIG GMIFS  TM
Sbjct: 377 -GFTVPPASMFTLTALTLIISLTVYEKLLVPLLRSITRNQRGINILQRIGTGMIFSLITM 436

Query: 443 VVAALVERKRLSQTAD--AMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMRSLGI 502
           ++AALVE++RL +T +   MSV WLAPQF +IG  D F LVGLQEYFY QVPDSMRSLGI
Sbjct: 437 IIAALVEKQRLDRTNNNKPMSVIWLAPQFMVIGFADAFTLVGLQEYFYHQVPDSMRSLGI 496

Query: 503 AFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNSSRLDNFYWLIAGIVAVDLCV 562
           AFYLSV GAA+FL++LLIT VD + +  SGKSWFG+DLNSSRLD FYW +AG++A ++CV
Sbjct: 497 AFYLSVIGAASFLNNLLITAVDTLAENFSGKSWFGKDLNSSRLDRFYWFLAGVIAANICV 556

Query: 563 YVLLARRYTYKSVQ 575
           +V++A+R  YKSVQ
Sbjct: 557 FVIVAKRCPYKSVQ 569

BLAST of HG10020354.1 vs. ExPASy Swiss-Prot
Match: Q9M331 (Protein NRT1/ PTR FAMILY 5.7 OS=Arabidopsis thaliana OX=3702 GN=NPF5.7 PE=2 SV=2)

HSP 1 Score: 737.3 bits (1902), Expect = 1.4e-211
Identity = 357/583 (61.23%), Postives = 457/583 (78.39%), Query Frame = 0

Query: 9   ERELLGDEDEPKNKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGI 68
           E   +  E +    D Q WV DSS D +G +PLRA TG W+++LFII IEFSERLSYFGI
Sbjct: 2   EHNKVDTEPQDSYDDQQKWVLDSSTDSRGEIPLRAQTGAWRAALFIIGIEFSERLSYFGI 61

Query: 69  ATSLVIYLTRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLL 128
           +T+LV+YLT ++H+DLK A +N NYW+GVTTLMPLLGGF+ADAYLGR+ TVLL+T IYL+
Sbjct: 62  STNLVVYLTTILHQDLKMAVKNTNYWSGVTTLMPLLGGFVADAYLGRYGTVLLATTIYLM 121

Query: 129 GLSLLTMSTMVPSLKPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFD 188
           GL LLT+S  +P LK C  + C EPRK HEI FF AIYLISIGTGGHKPSLESFGADQF+
Sbjct: 122 GLILLTLSWFIPGLKACHEDMCVEPRKAHEIAFFIAIYLISIGTGGHKPSLESFGADQFE 181

Query: 189 DDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFL 248
           D HPEERK KMS+FNWWN+GLCAG++  VT+IVY+++ +GWG+A +ILT VMA S  IF 
Sbjct: 182 DGHPEERKMKMSYFNWWNAGLCAGILTAVTVIVYIEDRIGWGVASIILTIVMATSFFIFR 241

Query: 249 LGRSVYRFRAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQGRLLSHTKN 308
           +G+  YR+RAP GSPLTP+LQV VAA   RNL  P  SS L+EL   +  +GRLLS +KN
Sbjct: 242 IGKPFYRYRAPSGSPLTPMLQVFVAAIAKRNLPCPSDSSLLHELTNEEYTKGRLLSSSKN 301

Query: 309 LKFLDKAAIIEERSGNSEG-KEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQAS 368
           LKFLDKAA+IE+R+ N++  K+  WRLATVT+VEE+KL++NMIPIW  +L FG+C  Q+S
Sbjct: 302 LKFLDKAAVIEDRNENTKAEKQSPWRLATVTKVEEVKLLINMIPIWFFTLAFGVCATQSS 361

Query: 369 TFFVKQSGTLDRKL-GNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGIS 428
           T F+KQ+  +DR + G +FI+P +S++ L A  +I++V IY+K+LVPLLR+ TGNERGIS
Sbjct: 362 TLFIKQAIIMDRHITGTSFIVPPASLFSLIALSIIITVTIYEKLLVPLLRRATGNERGIS 421

Query: 429 ILQRIGIGMIFSFTTMVVAALVERKRLSQTAD-------AMSVFWLAPQFFIIGIGDGFA 488
           ILQRIG+GM+FS   M++AAL+E+KRL    +        +S  WLAPQF ++G+ D F 
Sbjct: 422 ILQRIGVGMVFSLFAMIIAALIEKKRLDYAKEHHMNKTMTLSAIWLAPQFLVLGVADAFT 481

Query: 489 LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLN 548
           LVGLQEYFYDQVPDSMRSLGIAFYLSV GAA+F+++LLIT+ D + ++ SGK WFG+DLN
Sbjct: 482 LVGLQEYFYDQVPDSMRSLGIAFYLSVLGAASFVNNLLITVSDHLAEEISGKGWFGKDLN 541

Query: 549 SSRLDNFYWLIAGIVAVDLCVYVLLARRYTYKSVQKT--TVAD 581
           SSRLD FYW++A + A ++C +V++A RYTYK+VQ +   VAD
Sbjct: 542 SSRLDRFYWMLAALTAANICCFVIVAMRYTYKTVQPSLAVVAD 584

BLAST of HG10020354.1 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 4.9e-124
Identity = 237/561 (42.25%), Postives = 340/561 (60.61%), Query Frame = 0

Query: 25  QIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVMHEDL 84
           +++  D SVD  GN PL+  TG WK+  FI+  E  ERL+Y+GIA +L+ YLT  +H+  
Sbjct: 22  KLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGN 81

Query: 85  KTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTMVPSLKP 144
            +AA NV  W G   L PL+G  LADAY GR+ T+   + IY +G+S LT+S  VP+LKP
Sbjct: 82  VSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKP 141

Query: 145 --CGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERKQKMSFF 204
             C  + C         +FF  +YLI++GTGG KP + SFGADQFDD    ER +K SFF
Sbjct: 142 AECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFF 201

Query: 205 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRFRAPLGS 264
           NW+   +  G +   +L+V++QE+ GWG+   I T  M L++A F  G  +YRF+ P GS
Sbjct: 202 NWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGS 261

Query: 265 PLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDK--FQGRLLSHTKNLKFLDKAAIIEE 324
           P+T + QV+VA+FR  ++  P  ++ LYE Q  +      R + HT + ++LDKAA+I E
Sbjct: 262 PITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISE 321

Query: 325 RSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGTLDRK 384
               S     +WRL TVT+VEELK+++ M PIW + + F    AQ ST FV+Q   ++ K
Sbjct: 322 EESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCK 381

Query: 385 LGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMIFSFT 444
           +G +F LP +++     A +I+ V +YD+ +VPL RK TG ++G + +QR+GIG+  S  
Sbjct: 382 IG-SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVL 441

Query: 445 TMVVAALVERKRLSQTAD----------AMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQ 504
            M  AA+VE  RL    D           +SV W  PQ+FI+G  + F  +G  E+FYDQ
Sbjct: 442 CMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQ 501

Query: 505 VPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNSSRLDNFYWLI 564
            PD+MRSL  A  L  N   N+LSSL++T+V   T ++  + W  ++LNS  LD F+WL+
Sbjct: 502 SPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFWLL 561

Query: 565 AGIVAVDLCVYVLLARRYTYK 572
           AG+  V++ VY   A RY  K
Sbjct: 562 AGLSLVNMAVYFFSAARYKQK 581

BLAST of HG10020354.1 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 444.1 bits (1141), Expect = 2.4e-123
Identity = 239/566 (42.23%), Postives = 347/566 (61.31%), Query Frame = 0

Query: 25  QIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVMHEDL 84
           +++  D +VD +G   L + TG W++  F++  E  ER++++GIA++LV YLT+ +HED 
Sbjct: 5   KVYTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDT 64

Query: 85  KTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTMVPSLKP 144
            ++ RNVN W+G   + P+ G ++AD+Y+GRF T   S++IY+LG+ LLTM+  V SL+P
Sbjct: 65  ISSVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLRP 124

Query: 145 -CGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERKQKMSFFN 204
            C +  C +   +    F+ ++Y I+IG GG KP++ +FGADQFD    EE+KQK+SFFN
Sbjct: 125 TCENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVSFFN 184

Query: 205 WWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRFRAPLGSP 264
           WW      G +F    +VY+QE++GWG+   I T  + +SL +F +G   YR +      
Sbjct: 185 WWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIKTDN 244

Query: 265 LT-PLLQVIVAAFRNRNLAYPPHSSHLYELQT-TDKFQGR-LLSHTKNLKFLDKAAIIEE 324
           L   L+QV +AAF+NR L  P     LYEL +   K  G+  + HT   +FLDKAAI   
Sbjct: 245 LAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAI--- 304

Query: 325 RSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGTLDRK 384
                  K  +    TVT+VE  K VL +I IW+ +L      AQ +T FVKQ  TLDRK
Sbjct: 305 -------KTSSRVPCTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRK 364

Query: 385 LGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMIFSFT 444
           +G+NF +PA+S+       M+LSV +YD+  VP +RK TGN RGI++LQR+G+G      
Sbjct: 365 IGSNFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIV 424

Query: 445 TMVVAALVERKRLSQTAD----------AMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQ 504
            + +A+ VE KR+    +           MS+FWL PQ+ ++GIGD F  +GL E+FYDQ
Sbjct: 425 AIAIASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQ 484

Query: 505 VPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNSSRLDNFYWLI 564
            P+ M+SLG  F+ S  G  NFL+S L+TM+D+IT K  GKSW G +LN SRLD +Y  +
Sbjct: 485 SPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFL 544

Query: 565 AGIVAVDLCVYVLLARRYTYKSVQKT 577
             I  V++ ++V  A +Y YKS   T
Sbjct: 545 VVISIVNMGLFVWAASKYVYKSDDDT 560

BLAST of HG10020354.1 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 4.6e-122
Identity = 226/563 (40.14%), Postives = 342/563 (60.75%), Query Frame = 0

Query: 22  KDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVMH 81
           ++  ++  D +VD   N   +  TG WK+  FI+  E  ERL+Y+G+ T+LV YL   ++
Sbjct: 2   EEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLN 61

Query: 82  EDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTMVPS 141
           +   TAA NV  W+G   + PL+G F+ADAYLGR+ T+     IY+ G++LLT+S  VP 
Sbjct: 62  QGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPG 121

Query: 142 LKP--CGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERKQKM 201
           LKP  C ++TC  P      +FF A+Y+I++GTGG KP + SFGADQFD++   E+ +K 
Sbjct: 122 LKPGNCNADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 181

Query: 202 SFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRFRAP 261
           SFFNW+   +  G +   T++V++Q +VGWG    + T  M +++  F  G   YR + P
Sbjct: 182 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 241

Query: 262 LGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTD-KFQG-RLLSHTKNLKFLDKAAI 321
            GSPLT + QVIVAAFR  ++  P   S L+E    +   +G R L HT NLKF DKAA+
Sbjct: 242 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 301

Query: 322 IEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGTL 381
             +     +G+   WRL +VT+VEELK ++ ++P+W T + F    +Q ST FV Q  T+
Sbjct: 302 ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 361

Query: 382 DRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMIF 441
           D+ +G NF +P++S+       ++    +YD+ ++PL RK T NERG + LQR+GIG++ 
Sbjct: 362 DQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVV 421

Query: 442 SFTTMVVAALVERKRL---------SQTADAMSVFWLAPQFFIIGIGDGFALVGLQEYFY 501
           S   M+ A ++E  RL          Q    MS+FW  PQ+ +IG  + F  +G  E+FY
Sbjct: 422 SIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFY 481

Query: 502 DQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNSSRLDNFYW 561
           DQ PD+MRSL  A  L+     N+LS++L+T+V +ITKK+    W  ++LN   LD F++
Sbjct: 482 DQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFY 541

Query: 562 LIAGIVAVDLCVYVLLARRYTYK 572
           L+A +  ++  VY+ +++RY YK
Sbjct: 542 LLATLSFLNFLVYLWISKRYKYK 563

BLAST of HG10020354.1 vs. ExPASy TrEMBL
Match: A0A5A7TPU6 (Protein NRT1/ PTR FAMILY 5.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004790 PE=3 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 5.4e-299
Identity = 516/577 (89.43%), Postives = 546/577 (94.63%), Query Frame = 0

Query: 17  DEPKNKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYL 76
           D+   +D+QIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYL
Sbjct: 4   DQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYL 63

Query: 77  TRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMS 136
           TRV+HEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVL+ST+IYLLGLSLLTMS
Sbjct: 64  TRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLISTVIYLLGLSLLTMS 123

Query: 137 TMVPSLKPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERK 196
           T+VPSLKPC  ETC+EPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERK
Sbjct: 124 TLVPSLKPCDGETCQEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERK 183

Query: 197 QKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRF 256
           QKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGM GVILTSVMA+SLAIFLLGR VYR+
Sbjct: 184 QKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRY 243

Query: 257 RAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQGRLLSHTKNLKFLDKAA 316
           RAP GSPLTPLLQVI+AAFR R L YPPHSS L+E+Q+ DKFQGRLLSHTKNL+FLDKAA
Sbjct: 244 RAPSGSPLTPLLQVIIAAFRKRKLDYPPHSSQLHEVQSGDKFQGRLLSHTKNLRFLDKAA 303

Query: 317 IIEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGT 376
           I+EER GNS+ K+GAWRLATVTRVEELKLVLNMIPIWITSLPF ICVAQASTFF+KQ GT
Sbjct: 304 IVEER-GNSKEKQGAWRLATVTRVEELKLVLNMIPIWITSLPFSICVAQASTFFIKQCGT 363

Query: 377 LDRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMI 436
           LDRK+GN F++P SSMYCLAAAGMI+SVAIYDK+LVPLLRKTTGNERGISILQRIGIGM+
Sbjct: 364 LDRKIGNTFVIPVSSMYCLAAAGMIISVAIYDKLLVPLLRKTTGNERGISILQRIGIGMV 423

Query: 437 FSFTTMVVAALVERKRLSQTADAMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMR 496
           FSFTTMVV+ALVERKRLS T   MSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMR
Sbjct: 424 FSFTTMVVSALVERKRLSHTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMR 483

Query: 497 SLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNSSRLDNFYWLIAGIVAV 556
           SLGIAFYLSVNGAANFLSS +IT+VDRITKKSSGKSWFG+DLNSSRLDNFYWLIAGIVAV
Sbjct: 484 SLGIAFYLSVNGAANFLSSFIITIVDRITKKSSGKSWFGDDLNSSRLDNFYWLIAGIVAV 543

Query: 557 DLCVYVLLARRYTYKSVQKTTVADCYDGKGPHATSPV 594
           DLCVYV LA RYTYKSVQKT V DCYD KGP+A S V
Sbjct: 544 DLCVYVFLAHRYTYKSVQKTAVGDCYDDKGPNADSAV 579

BLAST of HG10020354.1 vs. ExPASy TrEMBL
Match: A0A1S3B6K9 (protein NRT1/ PTR FAMILY 5.6 OS=Cucumis melo OX=3656 GN=LOC103486351 PE=3 SV=1)

HSP 1 Score: 1035.0 bits (2675), Expect = 1.2e-298
Identity = 522/594 (87.88%), Postives = 554/594 (93.27%), Query Frame = 0

Query: 1   MKT-DQLRKERELLGDEDEPKNKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEF 60
           MKT +QL K +      D+   +D+QIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEF
Sbjct: 1   MKTNNQLEKMK-----RDQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEF 60

Query: 61  SERLSYFGIATSLVIYLTRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTV 120
           SERLSYFGIATSLVIYLTRV+HEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTV
Sbjct: 61  SERLSYFGIATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTV 120

Query: 121 LLSTIIYLLGLSLLTMSTMVPSLKPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSL 180
           L+ST+IYLLGLSLLTMST+VPSLKPC  ETC+EPRKVHEILFFTAIYLISIGTGGHKPSL
Sbjct: 121 LISTVIYLLGLSLLTMSTLVPSLKPCDGETCQEPRKVHEILFFTAIYLISIGTGGHKPSL 180

Query: 181 ESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSV 240
           ESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGM GVILTSV
Sbjct: 181 ESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSV 240

Query: 241 MALSLAIFLLGRSVYRFRAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQ 300
           MA+SLAIFLLGR VYR+RAP GSPLTPLLQVI+AAFR R L YPPHSS L+E+Q+ DKFQ
Sbjct: 241 MAISLAIFLLGRPVYRYRAPSGSPLTPLLQVIIAAFRKRKLDYPPHSSQLHEVQSGDKFQ 300

Query: 301 GRLLSHTKNLKFLDKAAIIEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPF 360
           GRLLSHTKNL+FLDKAAI+EER GNS+ K+GAWRLATVTRVEELKLVLNMIPIWITSLPF
Sbjct: 301 GRLLSHTKNLRFLDKAAIVEER-GNSKEKQGAWRLATVTRVEELKLVLNMIPIWITSLPF 360

Query: 361 GICVAQASTFFVKQSGTLDRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTT 420
            ICVAQASTFF+KQ GTLDRK+GN F++P SSMYCLAAAGMI+SVAIYDK+LVPLLRKTT
Sbjct: 361 SICVAQASTFFIKQCGTLDRKIGNTFVIPVSSMYCLAAAGMIISVAIYDKLLVPLLRKTT 420

Query: 421 GNERGISILQRIGIGMIFSFTTMVVAALVERKRLSQTADAMSVFWLAPQFFIIGIGDGFA 480
           GNERGISILQRIGIGM+FSFTTMVV+ALVERKRLS T   MSVFWLAPQFFIIGIGDGFA
Sbjct: 421 GNERGISILQRIGIGMVFSFTTMVVSALVERKRLSHTTHPMSVFWLAPQFFIIGIGDGFA 480

Query: 481 LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLN 540
           LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSS +IT+VDRITKKSSGKSWFG+DLN
Sbjct: 481 LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFIITIVDRITKKSSGKSWFGDDLN 540

Query: 541 SSRLDNFYWLIAGIVAVDLCVYVLLARRYTYKSVQKTTVADCYDGKGPHATSPV 594
           SSRLDNFYWLIAGIVAVDLCVYV LA RYTYKSVQKT V DCYD KGP+A S V
Sbjct: 541 SSRLDNFYWLIAGIVAVDLCVYVFLAHRYTYKSVQKTAVGDCYDDKGPNADSAV 588

BLAST of HG10020354.1 vs. ExPASy TrEMBL
Match: A0A6J1CW84 (protein NRT1/ PTR FAMILY 5.6-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014970 PE=3 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 5.8e-277
Identity = 485/596 (81.38%), Postives = 535/596 (89.77%), Query Frame = 0

Query: 6   LRKERELLGDEDEPK-------NKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIE 65
           ++ ERE   D+ + K       N  N   V+DSSVDHKG +PLRASTGVWK+SLFIIAIE
Sbjct: 1   MKTEREEFTDQPKNKIHHNNNNNNHNHDLVHDSSVDHKGRIPLRASTGVWKASLFIIAIE 60

Query: 66  FSERLSYFGIATSLVIYLTRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFST 125
           FSERLSYFGIATSLVIYLT+V+ +DLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFST
Sbjct: 61  FSERLSYFGIATSLVIYLTKVIRQDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFST 120

Query: 126 VLLSTIIYLLGLSLLTMSTMVPSLKPCGSE-TCEEPRKVHEILFFTAIYLISIGTGGHKP 185
           VL STI+YLLGLSLLT+ST+VPSLK CG+E TC++PRK+HE+LFFTAIY IS+GTGGHKP
Sbjct: 121 VLASTIVYLLGLSLLTVSTLVPSLKACGAEITCDQPRKIHEVLFFTAIYFISVGTGGHKP 180

Query: 186 SLESFGADQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILT 245
           SLESFGADQFDDDH EERKQKMSFFNWWNSGLCAGVI GVTLIVYVQ+HVGWG++G ILT
Sbjct: 181 SLESFGADQFDDDHAEERKQKMSFFNWWNSGLCAGVILGVTLIVYVQDHVGWGVSGAILT 240

Query: 246 SVMALSLAIFLLGRSVYRFRAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDK 305
           SVMA+SLAIFLLGR VYRFRAPLGSPLTPLLQV+VAAFRNRNL YPPH SHLYELQ+T  
Sbjct: 241 SVMAISLAIFLLGRPVYRFRAPLGSPLTPLLQVLVAAFRNRNLPYPPHPSHLYELQSTHN 300

Query: 306 FQGRLLSHTKNLKFLDKAAIIEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSL 365
            QGRLLSHTK LKFLDKAAIIEE +GNS GK+ AWRLATVTRVEELKL+LNMIPIWITSL
Sbjct: 301 IQGRLLSHTKKLKFLDKAAIIEE-TGNSSGKQSAWRLATVTRVEELKLILNMIPIWITSL 360

Query: 366 PFGICVAQASTFFVKQSGTLDRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRK 425
           PFGICVAQ STFF+KQ  TLDRK+GN+FI+PASSM+CLAAAGMI+SVAIYDK++VP+LRK
Sbjct: 361 PFGICVAQTSTFFIKQCLTLDRKIGNSFIMPASSMFCLAAAGMIVSVAIYDKVIVPILRK 420

Query: 426 TTGNERGISILQRIGIGMIFSFTTMVVAALVERKRLSQTAD-------AMSVFWLAPQFF 485
           TTGNERGISILQRIGIGM+FS T+M VAA+VERKRL    D        MSVFWLAPQF 
Sbjct: 421 TTGNERGISILQRIGIGMVFSLTSMAVAAVVERKRLDVVKDNPTKGSMEMSVFWLAPQFL 480

Query: 486 IIGIGDGFALVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSG 545
           IIGI DGFALVGLQEYFYDQVPDSMRS+GIA YLSVNGAANF+SSLLIT+VDRITKKS+ 
Sbjct: 481 IIGIADGFALVGLQEYFYDQVPDSMRSIGIALYLSVNGAANFVSSLLITVVDRITKKSND 540

Query: 546 KSWFGEDLNSSRLDNFYWLIAGIVAVDLCVYVLLARRYTYKSVQKTTVADCYDGKG 587
           KSWFG+DLNSSRLDNFYWLIA +VAV+LCVYV LARRY+YK++QKTTVADCYDG G
Sbjct: 541 KSWFGDDLNSSRLDNFYWLIAAVVAVNLCVYVFLARRYSYKTLQKTTVADCYDGDG 595

BLAST of HG10020354.1 vs. ExPASy TrEMBL
Match: A0A6J1J2X0 (protein NRT1/ PTR FAMILY 5.6-like OS=Cucurbita maxima OX=3661 GN=LOC111482191 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 3.4e-269
Identity = 468/593 (78.92%), Postives = 527/593 (88.87%), Query Frame = 0

Query: 10  RELLGDEDEP-KNKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGI 69
           RE     DEP ++  +Q  V+DSSVDHKG LPLRASTGVW+SSLFIIAIEFSERLSYFGI
Sbjct: 5   REAAEFADEPDQHTHDQNLVHDSSVDHKGKLPLRASTGVWRSSLFIIAIEFSERLSYFGI 64

Query: 70  ATSLVIYLTRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLL 129
           +TSL+IYLT+ MHEDLKTAA NVNYWTGVTTLMPLLGGFLADAYLGR+STV+LST++YLL
Sbjct: 65  STSLIIYLTKAMHEDLKTAATNVNYWTGVTTLMPLLGGFLADAYLGRYSTVILSTVVYLL 124

Query: 130 GLSLLTMSTMVPSLKPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFD 189
           GLSL+T+ST+VP LK CGSE C +PRKVHE+LFFTAIY IS+GTGGHKPSLESFGADQFD
Sbjct: 125 GLSLMTLSTLVPRLKACGSEICNKPRKVHEVLFFTAIYFISVGTGGHKPSLESFGADQFD 184

Query: 190 DDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFL 249
           D+HP+ERKQKMSFFNWWNSGLCAG+I GVTLIVYV++H+GWG+AGVILTS+MALSLA+FL
Sbjct: 185 DNHPDERKQKMSFFNWWNSGLCAGIILGVTLIVYVEDHMGWGIAGVILTSIMALSLAVFL 244

Query: 250 LGRSVYRFRAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQGRLLSHTKN 309
            G SVYR+R PLGSPLTPLLQV VAAFRNRNL YPPHSSHLYE+QT D+FQ RLLSHTK 
Sbjct: 245 FGSSVYRYRVPLGSPLTPLLQVAVAAFRNRNLPYPPHSSHLYEVQTADRFQARLLSHTKK 304

Query: 310 LKFLDKAAIIEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQAST 369
           LKFLDKAAIIEE  GNS+GK+G WRLATVTRVEELKL+LNMIPIWI SLPF I VAQ+ST
Sbjct: 305 LKFLDKAAIIEE-GGNSDGKQGPWRLATVTRVEELKLILNMIPIWIVSLPFAIVVAQSST 364

Query: 370 FFVKQSGTLDRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISIL 429
           FFVKQ   +DRK+G++FI+PASSM+CLAA GMI+ V IYD++LVP LRKTTGNERGI+IL
Sbjct: 365 FFVKQCVIMDRKIGSSFIMPASSMFCLAALGMIIFVVIYDRLLVPFLRKTTGNERGITIL 424

Query: 430 QRIGIGMIFSFTTMVVAALVERKRL--------SQTADAMSVFWLAPQFFIIGIGDGFAL 489
           QRIGIGM+FSFTTM+VAA+VERKR+          +A AMSVFWLAPQF IIGIGD FAL
Sbjct: 425 QRIGIGMVFSFTTMIVAAVVERKRVGLVKNDPTGGSAGAMSVFWLAPQFIIIGIGDAFAL 484

Query: 490 VGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNS 549
           VGLQEYFYDQVPDSMRSLGIAFYLSV+GAANF+SS LIT+VD IT+KS G+SWFGEDLN+
Sbjct: 485 VGLQEYFYDQVPDSMRSLGIAFYLSVSGAANFVSSWLITIVDNITRKSGGQSWFGEDLNT 544

Query: 550 SRLDNFYWLIAGIVAVDLCVYVLLARRYTYKSVQKTTVADCYDGKGPHATSPV 594
           SRLDNFY+L+A IVAVDLCVYV+LARRYTYKSVQKTTV  C D   P  +S V
Sbjct: 545 SRLDNFYFLLALIVAVDLCVYVILARRYTYKSVQKTTVGACSDADAPDTSSAV 596

BLAST of HG10020354.1 vs. ExPASy TrEMBL
Match: A0A6J1FT20 (protein NRT1/ PTR FAMILY 5.6-like OS=Cucurbita moschata OX=3662 GN=LOC111447038 PE=3 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 6.4e-268
Identity = 472/578 (81.66%), Postives = 519/578 (89.79%), Query Frame = 0

Query: 18  EPKNK-DNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYL 77
           E +N+ D+QI V+DSSVDHK  LPLRASTGVWK+SLFIIAIEFSERLSYFGIATSLVIYL
Sbjct: 4   EAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYL 63

Query: 78  TRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMS 137
           TRV+ +DLKTAARNVNYWTGVTTLMPL GGFLADAYLGRFSTVL+STI+YL GLSLLT+S
Sbjct: 64  TRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLS 123

Query: 138 TMVPSLKPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERK 197
           T+VPSLK CGSE C+EPRK+HE+LFFTAIYLIS+GTGGHKPSLESFGADQFDDDH EERK
Sbjct: 124 TLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERK 183

Query: 198 QKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRF 257
           QKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMA SLAIFL+GR VYR+
Sbjct: 184 QKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRY 243

Query: 258 RAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQGRLLSHTKNLKFLDKAA 317
           RAPLGSPLTPLLQV+VAAF  RNL YP +S+HLYE+Q TDK  GRLL+HT  LKFLDKAA
Sbjct: 244 RAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLKFLDKAA 303

Query: 318 IIEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGT 377
           I+ E S NS GK+  WRLATVTRVEELKLVLNMIPIWI S+PFGICVAQ  TFF+KQ  T
Sbjct: 304 IVGETS-NSAGKQSPWRLATVTRVEELKLVLNMIPIWIASIPFGICVAQTYTFFIKQCAT 363

Query: 378 LDRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMI 437
           LDRK+G  FI+PASSM+CLAA GMI+SVAIYD++LVP+LRKTTGNERGI+ILQRIGIGMI
Sbjct: 364 LDRKIGKTFIIPASSMFCLAAVGMIISVAIYDRLLVPILRKTTGNERGITILQRIGIGMI 423

Query: 438 FSFTTMVVAALVERKRL-------SQTADAMSVFWLAPQFFIIGIGDGFALVGLQEYFYD 497
           FSF  M VA LVERKRL       ++ + AMSVFWL PQF IIGI DGFALVGLQEYFYD
Sbjct: 424 FSFMAMSVAGLVERKRLGVVKGSPTKGSGAMSVFWLGPQFLIIGIADGFALVGLQEYFYD 483

Query: 498 QVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSS-GKSWFGEDLNSSRLDNFYW 557
           QVPDSMRSLGIAFYLSVNGAANF+SSLLIT VDRIT KSS GKSWFG++LN+SRLD+FYW
Sbjct: 484 QVPDSMRSLGIAFYLSVNGAANFVSSLLITAVDRITGKSSGGKSWFGDNLNTSRLDHFYW 543

Query: 558 LIAGIVAVDLCVYVLLARRYTYKSVQKTTVADCYDGKG 587
           LIAGIVAV+LC +VL AR+Y YKSVQ+TTVADC+DG G
Sbjct: 544 LIAGIVAVNLCFFVLFARQYAYKSVQRTTVADCFDGDG 580

BLAST of HG10020354.1 vs. TAIR 10
Match: AT2G37900.1 (Major facilitator superfamily protein )

HSP 1 Score: 746.1 bits (1925), Expect = 2.1e-215
Identity = 360/554 (64.98%), Postives = 449/554 (81.05%), Query Frame = 0

Query: 23  DNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVMHE 82
           D Q WV DSS+D +G +PLRA TG W+++LFIIAIEFSERLSYFG+AT+LV+YLT ++++
Sbjct: 17  DQQKWVLDSSLDSRGRVPLRARTGAWRAALFIIAIEFSERLSYFGLATNLVVYLTTILNQ 76

Query: 83  DLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTMVPSL 142
           DLK A RNVNYW+GVTTLMPLLGGF+ADAYLGR++TVL++T IYL+GL LLTMS  +P L
Sbjct: 77  DLKMAIRNVNYWSGVTTLMPLLGGFIADAYLGRYATVLVATTIYLMGLVLLTMSWFIPGL 136

Query: 143 KPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERKQKMSFF 202
           KPC  E C EPRK HE+ FF AIYLISIGTGGHKPSLESFGADQFDDDH EERK KMSFF
Sbjct: 137 KPCHQEVCVEPRKAHEVAFFIAIYLISIGTGGHKPSLESFGADQFDDDHVEERKMKMSFF 196

Query: 203 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRFRAPLGS 262
           NWWN  LCAG++  VT + Y+++ VGWG+AG+ILT VMA+SL IF +G+  YR+R P GS
Sbjct: 197 NWWNVSLCAGILTAVTAVAYIEDRVGWGVAGIILTVVMAISLIIFFIGKPFYRYRTPSGS 256

Query: 263 PLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQGRLLSHTKNLKFLDKAAIIEERS 322
           PLTP+LQV VAA   RNL YP   S L+E+  T+   GRLL HT++LKFLDKAAIIE+++
Sbjct: 257 PLTPILQVFVAAIAKRNLPYPSDPSLLHEVSKTEFTSGRLLCHTEHLKFLDKAAIIEDKN 316

Query: 323 GNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGTLDRKLG 382
             +  K+  WRL T+T+VEE KL++N+IPIW ++L FGIC  QASTFF+KQ+ T+DR +G
Sbjct: 317 PLALEKQSPWRLLTLTKVEETKLIINVIPIWFSTLAFGICATQASTFFIKQAITMDRHIG 376

Query: 383 NNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMIFSFTTM 442
             F +P +SM+ L A  +I+S+ +Y+K+LVPLLR  T N+RGI+ILQRIG GMIFS  TM
Sbjct: 377 -GFTVPPASMFTLTALTLIISLTVYEKLLVPLLRSITRNQRGINILQRIGTGMIFSLITM 436

Query: 443 VVAALVERKRLSQTAD--AMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMRSLGI 502
           ++AALVE++RL +T +   MSV WLAPQF +IG  D F LVGLQEYFY QVPDSMRSLGI
Sbjct: 437 IIAALVEKQRLDRTNNNKPMSVIWLAPQFMVIGFADAFTLVGLQEYFYHQVPDSMRSLGI 496

Query: 503 AFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNSSRLDNFYWLIAGIVAVDLCV 562
           AFYLSV GAA+FL++LLIT VD + +  SGKSWFG+DLNSSRLD FYW +AG++A ++CV
Sbjct: 497 AFYLSVIGAASFLNNLLITAVDTLAENFSGKSWFGKDLNSSRLDRFYWFLAGVIAANICV 556

Query: 563 YVLLARRYTYKSVQ 575
           +V++A+R  YKSVQ
Sbjct: 557 FVIVAKRCPYKSVQ 569

BLAST of HG10020354.1 vs. TAIR 10
Match: AT3G53960.1 (Major facilitator superfamily protein )

HSP 1 Score: 737.3 bits (1902), Expect = 9.9e-213
Identity = 357/583 (61.23%), Postives = 457/583 (78.39%), Query Frame = 0

Query: 9   ERELLGDEDEPKNKDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGI 68
           E   +  E +    D Q WV DSS D +G +PLRA TG W+++LFII IEFSERLSYFGI
Sbjct: 2   EHNKVDTEPQDSYDDQQKWVLDSSTDSRGEIPLRAQTGAWRAALFIIGIEFSERLSYFGI 61

Query: 69  ATSLVIYLTRVMHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLL 128
           +T+LV+YLT ++H+DLK A +N NYW+GVTTLMPLLGGF+ADAYLGR+ TVLL+T IYL+
Sbjct: 62  STNLVVYLTTILHQDLKMAVKNTNYWSGVTTLMPLLGGFVADAYLGRYGTVLLATTIYLM 121

Query: 129 GLSLLTMSTMVPSLKPCGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFD 188
           GL LLT+S  +P LK C  + C EPRK HEI FF AIYLISIGTGGHKPSLESFGADQF+
Sbjct: 122 GLILLTLSWFIPGLKACHEDMCVEPRKAHEIAFFIAIYLISIGTGGHKPSLESFGADQFE 181

Query: 189 DDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFL 248
           D HPEERK KMS+FNWWN+GLCAG++  VT+IVY+++ +GWG+A +ILT VMA S  IF 
Sbjct: 182 DGHPEERKMKMSYFNWWNAGLCAGILTAVTVIVYIEDRIGWGVASIILTIVMATSFFIFR 241

Query: 249 LGRSVYRFRAPLGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDKFQGRLLSHTKN 308
           +G+  YR+RAP GSPLTP+LQV VAA   RNL  P  SS L+EL   +  +GRLLS +KN
Sbjct: 242 IGKPFYRYRAPSGSPLTPMLQVFVAAIAKRNLPCPSDSSLLHELTNEEYTKGRLLSSSKN 301

Query: 309 LKFLDKAAIIEERSGNSEG-KEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQAS 368
           LKFLDKAA+IE+R+ N++  K+  WRLATVT+VEE+KL++NMIPIW  +L FG+C  Q+S
Sbjct: 302 LKFLDKAAVIEDRNENTKAEKQSPWRLATVTKVEEVKLLINMIPIWFFTLAFGVCATQSS 361

Query: 369 TFFVKQSGTLDRKL-GNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGIS 428
           T F+KQ+  +DR + G +FI+P +S++ L A  +I++V IY+K+LVPLLR+ TGNERGIS
Sbjct: 362 TLFIKQAIIMDRHITGTSFIVPPASLFSLIALSIIITVTIYEKLLVPLLRRATGNERGIS 421

Query: 429 ILQRIGIGMIFSFTTMVVAALVERKRLSQTAD-------AMSVFWLAPQFFIIGIGDGFA 488
           ILQRIG+GM+FS   M++AAL+E+KRL    +        +S  WLAPQF ++G+ D F 
Sbjct: 422 ILQRIGVGMVFSLFAMIIAALIEKKRLDYAKEHHMNKTMTLSAIWLAPQFLVLGVADAFT 481

Query: 489 LVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLN 548
           LVGLQEYFYDQVPDSMRSLGIAFYLSV GAA+F+++LLIT+ D + ++ SGK WFG+DLN
Sbjct: 482 LVGLQEYFYDQVPDSMRSLGIAFYLSVLGAASFVNNLLITVSDHLAEEISGKGWFGKDLN 541

Query: 549 SSRLDNFYWLIAGIVAVDLCVYVLLARRYTYKSVQKT--TVAD 581
           SSRLD FYW++A + A ++C +V++A RYTYK+VQ +   VAD
Sbjct: 542 SSRLDRFYWMLAALTAANICCFVIVAMRYTYKTVQPSLAVVAD 584

BLAST of HG10020354.1 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 446.4 bits (1147), Expect = 3.5e-125
Identity = 237/561 (42.25%), Postives = 340/561 (60.61%), Query Frame = 0

Query: 25  QIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVMHEDL 84
           +++  D SVD  GN PL+  TG WK+  FI+  E  ERL+Y+GIA +L+ YLT  +H+  
Sbjct: 22  KLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGN 81

Query: 85  KTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTMVPSLKP 144
            +AA NV  W G   L PL+G  LADAY GR+ T+   + IY +G+S LT+S  VP+LKP
Sbjct: 82  VSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKP 141

Query: 145 --CGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERKQKMSFF 204
             C  + C         +FF  +YLI++GTGG KP + SFGADQFDD    ER +K SFF
Sbjct: 142 AECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFF 201

Query: 205 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRFRAPLGS 264
           NW+   +  G +   +L+V++QE+ GWG+   I T  M L++A F  G  +YRF+ P GS
Sbjct: 202 NWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGS 261

Query: 265 PLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTDK--FQGRLLSHTKNLKFLDKAAIIEE 324
           P+T + QV+VA+FR  ++  P  ++ LYE Q  +      R + HT + ++LDKAA+I E
Sbjct: 262 PITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISE 321

Query: 325 RSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGTLDRK 384
               S     +WRL TVT+VEELK+++ M PIW + + F    AQ ST FV+Q   ++ K
Sbjct: 322 EESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCK 381

Query: 385 LGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMIFSFT 444
           +G +F LP +++     A +I+ V +YD+ +VPL RK TG ++G + +QR+GIG+  S  
Sbjct: 382 IG-SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVL 441

Query: 445 TMVVAALVERKRLSQTAD----------AMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQ 504
            M  AA+VE  RL    D           +SV W  PQ+FI+G  + F  +G  E+FYDQ
Sbjct: 442 CMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQ 501

Query: 505 VPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNSSRLDNFYWLI 564
            PD+MRSL  A  L  N   N+LSSL++T+V   T ++  + W  ++LNS  LD F+WL+
Sbjct: 502 SPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFWLL 561

Query: 565 AGIVAVDLCVYVLLARRYTYK 572
           AG+  V++ VY   A RY  K
Sbjct: 562 AGLSLVNMAVYFFSAARYKQK 581

BLAST of HG10020354.1 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 444.1 bits (1141), Expect = 1.7e-124
Identity = 239/566 (42.23%), Postives = 347/566 (61.31%), Query Frame = 0

Query: 25  QIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVMHEDL 84
           +++  D +VD +G   L + TG W++  F++  E  ER++++GIA++LV YLT+ +HED 
Sbjct: 5   KVYTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDT 64

Query: 85  KTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTMVPSLKP 144
            ++ RNVN W+G   + P+ G ++AD+Y+GRF T   S++IY+LG+ LLTM+  V SL+P
Sbjct: 65  ISSVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLRP 124

Query: 145 -CGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERKQKMSFFN 204
            C +  C +   +    F+ ++Y I+IG GG KP++ +FGADQFD    EE+KQK+SFFN
Sbjct: 125 TCENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVSFFN 184

Query: 205 WWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRFRAPLGSP 264
           WW      G +F    +VY+QE++GWG+   I T  + +SL +F +G   YR +      
Sbjct: 185 WWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIKTDN 244

Query: 265 LT-PLLQVIVAAFRNRNLAYPPHSSHLYELQT-TDKFQGR-LLSHTKNLKFLDKAAIIEE 324
           L   L+QV +AAF+NR L  P     LYEL +   K  G+  + HT   +FLDKAAI   
Sbjct: 245 LAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAI--- 304

Query: 325 RSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGTLDRK 384
                  K  +    TVT+VE  K VL +I IW+ +L      AQ +T FVKQ  TLDRK
Sbjct: 305 -------KTSSRVPCTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRK 364

Query: 385 LGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMIFSFT 444
           +G+NF +PA+S+       M+LSV +YD+  VP +RK TGN RGI++LQR+G+G      
Sbjct: 365 IGSNFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIV 424

Query: 445 TMVVAALVERKRLSQTAD----------AMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQ 504
            + +A+ VE KR+    +           MS+FWL PQ+ ++GIGD F  +GL E+FYDQ
Sbjct: 425 AIAIASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQ 484

Query: 505 VPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNSSRLDNFYWLI 564
            P+ M+SLG  F+ S  G  NFL+S L+TM+D+IT K  GKSW G +LN SRLD +Y  +
Sbjct: 485 SPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFL 544

Query: 565 AGIVAVDLCVYVLLARRYTYKSVQKT 577
             I  V++ ++V  A +Y YKS   T
Sbjct: 545 VVISIVNMGLFVWAASKYVYKSDDDT 560

BLAST of HG10020354.1 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 439.9 bits (1130), Expect = 3.3e-123
Identity = 226/563 (40.14%), Postives = 342/563 (60.75%), Query Frame = 0

Query: 22  KDNQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVMH 81
           ++  ++  D +VD   N   +  TG WK+  FI+  E  ERL+Y+G+ T+LV YL   ++
Sbjct: 2   EEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLN 61

Query: 82  EDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTMVPS 141
           +   TAA NV  W+G   + PL+G F+ADAYLGR+ T+     IY+ G++LLT+S  VP 
Sbjct: 62  QGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPG 121

Query: 142 LKP--CGSETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPEERKQKM 201
           LKP  C ++TC  P      +FF A+Y+I++GTGG KP + SFGADQFD++   E+ +K 
Sbjct: 122 LKPGNCNADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 181

Query: 202 SFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMALSLAIFLLGRSVYRFRAP 261
           SFFNW+   +  G +   T++V++Q +VGWG    + T  M +++  F  G   YR + P
Sbjct: 182 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 241

Query: 262 LGSPLTPLLQVIVAAFRNRNLAYPPHSSHLYELQTTD-KFQG-RLLSHTKNLKFLDKAAI 321
            GSPLT + QVIVAAFR  ++  P   S L+E    +   +G R L HT NLKF DKAA+
Sbjct: 242 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 301

Query: 322 IEERSGNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFGICVAQASTFFVKQSGTL 381
             +     +G+   WRL +VT+VEELK ++ ++P+W T + F    +Q ST FV Q  T+
Sbjct: 302 ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 361

Query: 382 DRKLGNNFILPASSMYCLAAAGMILSVAIYDKILVPLLRKTTGNERGISILQRIGIGMIF 441
           D+ +G NF +P++S+       ++    +YD+ ++PL RK T NERG + LQR+GIG++ 
Sbjct: 362 DQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVV 421

Query: 442 SFTTMVVAALVERKRL---------SQTADAMSVFWLAPQFFIIGIGDGFALVGLQEYFY 501
           S   M+ A ++E  RL          Q    MS+FW  PQ+ +IG  + F  +G  E+FY
Sbjct: 422 SIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFY 481

Query: 502 DQVPDSMRSLGIAFYLSVNGAANFLSSLLITMVDRITKKSSGKSWFGEDLNSSRLDNFYW 561
           DQ PD+MRSL  A  L+     N+LS++L+T+V +ITKK+    W  ++LN   LD F++
Sbjct: 482 DQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFY 541

Query: 562 LIAGIVAVDLCVYVLLARRYTYK 572
           L+A +  ++  VY+ +++RY YK
Sbjct: 542 LLATLSFLNFLVYLWISKRYKYK 563