HG10020340.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10020340.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	tropinone reductase homolog
Location	Chr04: 30990408 .. 30992040 (+)
Sequence length	810
RNA-Seq Expression	HG10020340.1
Synteny	HG10020340.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACACCAGAAACCAAAGATGGTCTCTCAAAGGGATGACCGCTCTCGTCACCGGCGGCAGCCGTGGCATTGGGTACTCCTAATTTTCAATTACTACTTTTTTTTTTTCGATTGAATTCATGTAATGTCATTTCCTCTGTTCAGGCGAGCCACGGTGGAAGAACTTGCAGAATTTGGGGCCAGCATTCACACGTGCTGTCGGAGCCAGGAAGATTTGGAGAAATGTTTGGAAGAATGGGAAGAAATGGGTTTGAAAGTGAGTGGGTCTGTATGCAATGTGGAAAGCAGAGAACAGAGGAAGAAGCTTATGGAAACGGTGTCGTCTCTGTTCAATGGCAGCCTCAACATTCTGGTAATTAACAAAAAAAAAGGAAATAATGAAAATAATGGAGTTTTGAGGGTGTTGATTTTGATTGTTTGAATTAGGTGAATAATGCTGGGAGAACGTCGTCGTTGAAGATGGCGACAGAAGTAACAGAGGAAGAGATTTCGGGCGTAATGAGTACAAATTTTGAAGGGTCGTTCCATATTAGCCAACTTGCATATCCATTGCTGAAAGCTTCTGGTAATGGAAGCATCGTGTTCATATCTTCTGTTGCAGGCTTCATGTCCCTTCCTTACTCTACTCCTTATGCTGCCTCCAAAGGTAACCGAAATATTTCTTCACCATTACCTTTCTGCAACGAAAAAGGGAAAAGACAACAGCTTCCTACGCTTTCAAAATAATTATTGGCAATTGTATATGCACGTGACATGAAGTAAAAATAGTGTTTGATAAATATTAATATAAAAAATACTTAATGCATCTTTTAATACCTATGTTTGTTTATCTCATCAAATTATCTAATTATAATTTGTCAAAGAGTAAATTAAAAAGTGATTTTACATAACTTAATATATAGACAAAGTTACCAAATAACTTAAATGGTTAAAATTCTTTTACAATGGCCTTTTACTCTCATATAGGCAATTCAAAACAAGACCTAGCCAATAAATTTTGTAAATTATTTAATAAATTCTTAAGTTTTCAATTTTGTATATAGAAAATTTCTAAAAGATACTTTTTAATATTCACAAATCTATTAATGAAACATTAAGTTAGAATTTTAGTTCCATCATAAAATTGAAATTTGTATCTTTAAACTGATAGACCAAAGTTGTAATTTGAAGTGAAGATATTTTGATGTGTGTATTTTATAAATGGATTTGTTGATGAAGCTGCCATAAATCAAATGACAAAGAACTTGGCATGCGAATGGGCGAAAGATAACATCCGTGCAAACGCAGTGGCTCCATGGTTCATCAGAACTCGCTTTGTGGAGCCTCCCAATGTACAAATTTTATTCATTATATTTATATCAACGTCTCTTTCAAAATTAATTCATATATATATATATATATATATACAAGTTTATTATATATTACTAATTTTTGCAGGATGATCCAATACATGTAAAAACAATGGAGCACGTATTGTCAATAACGCCTCTTAAACGCGCTGGAGAGCCACATGAAGTTTCGTCAATGGTTGCATTTCTTTGTCTTCCAGCTGCGTCTTATATTACTGGACAACTTTTTGTTATTGATGGTGGCCACACTGTTAAGGCTTTCCCCATCCCCGATCTCTGA

mRNA sequence

ATGGCCAACACCAGAAACCAAAGATGGTCTCTCAAAGGGATGACCGCTCTCGTCACCGGCGGCAGCCGTGGCATTGGGCGAGCCACGGTGGAAGAACTTGCAGAATTTGGGGCCAGCATTCACACGTGCTGTCGGAGCCAGGAAGATTTGGAGAAATGTTTGGAAGAATGGGAAGAAATGGGTTTGAAAGTGAGTGGGTCTGTATGCAATGTGGAAAGCAGAGAACAGAGGAAGAAGCTTATGGAAACGGTGTCGTCTCTGTTCAATGGCAGCCTCAACATTCTGGTGAATAATGCTGGGAGAACGTCGTCGTTGAAGATGGCGACAGAAGTAACAGAGGAAGAGATTTCGGGCGTAATGAGTACAAATTTTGAAGGGTCGTTCCATATTAGCCAACTTGCATATCCATTGCTGAAAGCTTCTGGTAATGGAAGCATCGTGTTCATATCTTCTGTTGCAGGCTTCATGTCCCTTCCTTACTCTACTCCTTATGCTGCCTCCAAAGCTGCCATAAATCAAATGACAAAGAACTTGGCATGCGAATGGGCGAAAGATAACATCCGTGCAAACGCAGTGGCTCCATGGTTCATCAGAACTCGCTTTGTGGAGCCTCCCAATGATGATCCAATACATGTAAAAACAATGGAGCACGTATTGTCAATAACGCCTCTTAAACGCGCTGGAGAGCCACATGAAGTTTCGTCAATGGTTGCATTTCTTTGTCTTCCAGCTGCGTCTTATATTACTGGACAACTTTTTGTTATTGATGGTGGCCACACTGTTAAGGCTTTCCCCATCCCCGATCTCTGA

Coding sequence (CDS)

ATGGCCAACACCAGAAACCAAAGATGGTCTCTCAAAGGGATGACCGCTCTCGTCACCGGCGGCAGCCGTGGCATTGGGCGAGCCACGGTGGAAGAACTTGCAGAATTTGGGGCCAGCATTCACACGTGCTGTCGGAGCCAGGAAGATTTGGAGAAATGTTTGGAAGAATGGGAAGAAATGGGTTTGAAAGTGAGTGGGTCTGTATGCAATGTGGAAAGCAGAGAACAGAGGAAGAAGCTTATGGAAACGGTGTCGTCTCTGTTCAATGGCAGCCTCAACATTCTGGTGAATAATGCTGGGAGAACGTCGTCGTTGAAGATGGCGACAGAAGTAACAGAGGAAGAGATTTCGGGCGTAATGAGTACAAATTTTGAAGGGTCGTTCCATATTAGCCAACTTGCATATCCATTGCTGAAAGCTTCTGGTAATGGAAGCATCGTGTTCATATCTTCTGTTGCAGGCTTCATGTCCCTTCCTTACTCTACTCCTTATGCTGCCTCCAAAGCTGCCATAAATCAAATGACAAAGAACTTGGCATGCGAATGGGCGAAAGATAACATCCGTGCAAACGCAGTGGCTCCATGGTTCATCAGAACTCGCTTTGTGGAGCCTCCCAATGATGATCCAATACATGTAAAAACAATGGAGCACGTATTGTCAATAACGCCTCTTAAACGCGCTGGAGAGCCACATGAAGTTTCGTCAATGGTTGCATTTCTTTGTCTTCCAGCTGCGTCTTATATTACTGGACAACTTTTTGTTATTGATGGTGGCCACACTGTTAAGGCTTTCCCCATCCCCGATCTCTGA

Protein sequence

MANTRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEMGLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVMSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLACEWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAFLCLPAASYITGQLFVIDGGHTVKAFPIPDL

Homology

[Close]

BLAST of HG10020340.1 vs. NCBI nr
Match: XP_038904663.1 (tropinone reductase homolog [Benincasa hispida])

HSP 1 Score: 483.4 bits (1243), Expect = 1.3e-132
Identity = 239/269 (88.85%), Postives = 255/269 (94.80%), Query Frame = 0


Query: 1   MANTRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEM 60
           MAN RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGA++HTCCRSQE+L+KCL+EWEEM
Sbjct: 1   MANNRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGATVHTCCRSQENLDKCLKEWEEM 60

Query: 61  GLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVM 120
           GLKVSGSVC+V+SREQRKKLMETVSSLFNGSLNILVNNAG TS LK A EVTEEEI  VM
Sbjct: 61  GLKVSGSVCDVQSREQRKKLMETVSSLFNGSLNILVNNAGTTSELKTAAEVTEEEIWSVM 120

Query: 121 STNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLAC 180
           +TNFE SFH SQLAYPLLKASGNG+IVFISSV+G M+LPYSTPYAASKAAINQMTKNLAC
Sbjct: 121 NTNFEASFHSSQLAYPLLKASGNGNIVFISSVSGLMALPYSTPYAASKAAINQMTKNLAC 180

Query: 181 EWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAFL 240
           EWAKDNIR NAVAPW IRTRFVEPPNDDPI+VK+ME +LS+TPLKRAGEPHEVSSMVAFL
Sbjct: 181 EWAKDNIRVNAVAPWIIRTRFVEPPNDDPIYVKSMEQLLSVTPLKRAGEPHEVSSMVAFL 240

Query: 241 CLPAASYITGQLFVIDGGHTVKAFPIPDL 270
           CLPAASYITGQLFVIDGGHTVKA+PI DL
Sbjct: 241 CLPAASYITGQLFVIDGGHTVKAYPISDL 269

[Close]

BLAST of HG10020340.1 vs. NCBI nr
Match: XP_008442511.1 (PREDICTED: tropinone reductase homolog [Cucumis melo] >KAA0044114.1 tropinone reductase-like protein [Cucumis melo var. makuwa] >TYK25024.1 tropinone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 469.5 bits (1207), Expect = 1.9e-128
Identity = 234/270 (86.67%), Postives = 250/270 (92.59%), Query Frame = 0


Query: 1   MANTRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEM 60
           MAN RNQRWSLKG TALVTGGSRGIGRATVEELAEFGA +HTCCRSQEDL+KCL+EWEEM
Sbjct: 1   MANIRNQRWSLKGKTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEEM 60

Query: 61  GLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRT-SSLKMATEVTEEEISGV 120
           G +VSGSVC+V+S+EQRKKLMETVSSLFNG+LNILVNNAGRT SSLK   EVTEEEIS V
Sbjct: 61  GFEVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEEISSV 120

Query: 121 MSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLA 180
           MSTNFE S H SQLAYPLLKASGNGSIVFISSV+G M+LP+STPYAASKAAINQMTKNLA
Sbjct: 121 MSTNFEASLHFSQLAYPLLKASGNGSIVFISSVSGLMALPFSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAF 240
           CEWAKDNIR NAVAPW I+TRFVE  NDDP+HVK ME +LS+TPLKRAGEPHEVSSMVAF
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRFVESSNDDPMHVKGMEQILSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAFPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKA+PI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 270

[Close]

BLAST of HG10020340.1 vs. NCBI nr
Match: XP_004137777.1 (tropinone reductase homolog [Cucumis sativus] >KGN58842.1 hypothetical protein Csa_002580 [Cucumis sativus])

HSP 1 Score: 464.9 bits (1195), Expect = 4.6e-127
Identity = 231/270 (85.56%), Postives = 249/270 (92.22%), Query Frame = 0


Query: 1   MANTRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEM 60
           MAN RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGA +HTCCRSQEDL+KCL+EWE M
Sbjct: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60

Query: 61  GLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRT-SSLKMATEVTEEEISGV 120
           G KVSGSVC+V+S+EQRKKLMETVSSLFNG+LNILVNNAGRT SSLK   EVTEE+IS V
Sbjct: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120

Query: 121 MSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLA 180
           MSTNFE SFH SQLAYPLLKASGNGSIVFISSV+G  +LP+STPYAASKAAINQ+TKNLA
Sbjct: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180

Query: 181 CEWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAF 240
           CEWAKDNIR NAVAPW I+TR VE  NDDP+HVK +E +LS+TPLKRAGEPHEVSSMV F
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAFPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKA+PIPDL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270

[Close]

BLAST of HG10020340.1 vs. NCBI nr
Match: XP_022971600.1 (tropinone reductase homolog [Cucurbita maxima])

HSP 1 Score: 450.7 bits (1158), Expect = 9.0e-123
Identity = 225/269 (83.64%), Postives = 246/269 (91.45%), Query Frame = 0


Query: 1   MANT-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEE 60
           MANT R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA +HTC RSQ DL+KCL+EWEE
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGAIVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGV 120
           MGL VSGSVC+V SREQRK+LMETVSSLFNGSLNILVNNAG TS+ K + EVTEEE+S V
Sbjct: 61  MGLNVSGSVCDVHSREQRKRLMETVSSLFNGSLNILVNNAG-TSAYKSSLEVTEEELSSV 120

Query: 121 MSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLA 180
           MSTNFE SFH SQLA+PL+KASGNGSIVF+SSVAG MSLPYSTPYAASKAAINQ+T+NLA
Sbjct: 121 MSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNLA 180

Query: 181 CEWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAF 240
           CEWAKDNIR NAVAPW IRTRFV+PP DDPIHV+ M H+LS+TPLKRAGEPHEVSSMVAF
Sbjct: 181 CEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAFPIPD 269
           LCLPAASYITGQ+F IDGGHTV AFPIPD
Sbjct: 241 LCLPAASYITGQVFCIDGGHTVTAFPIPD 268

[Close]

BLAST of HG10020340.1 vs. NCBI nr
Match: XP_022941371.1 (tropinone reductase homolog [Cucurbita moschata])

HSP 1 Score: 446.8 bits (1148), Expect = 1.3e-121
Identity = 221/269 (82.16%), Postives = 246/269 (91.45%), Query Frame = 0


Query: 1   MANT-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEE 60
           MANT R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA++HTC RSQ DL+KCL+EWEE
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGV 120
           MG  VSGSVC+V SREQR +L+ETVSSLFNGSLNILVNNAG TS+ K + EVTEEE+S V
Sbjct: 61  MGFNVSGSVCDVHSREQRMQLIETVSSLFNGSLNILVNNAG-TSAYKSSLEVTEEELSSV 120

Query: 121 MSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLA 180
           MSTNFE +FH SQLA+PL+KASGNGSIVF+SSVAG MSLPYSTPYAASKAAINQ+T+NLA
Sbjct: 121 MSTNFEAAFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNLA 180

Query: 181 CEWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAF 240
           CEWAKDNIR NAVAPW IRTRFV+PP DDPIHV+ M H+LS+TPLKRAGEPHEVSSMVAF
Sbjct: 181 CEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAFPIPD 269
           LCLPAASYITGQ+F IDGGHTVKAFP+PD
Sbjct: 241 LCLPAASYITGQVFCIDGGHTVKAFPVPD 268

[Close]

BLAST of HG10020340.1 vs. ExPASy Swiss-Prot
Match: P50165 (Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 5.4e-86
Identity = 160/259 (61.78%), Postives = 198/259 (76.45%), Query Frame = 0


Query: 6   NQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEMGLKVS 65
           ++RWSL+GMTALVTGG+RGIG A VEELA FGA ++TC RSQ DL++CLE+W   G KVS
Sbjct: 10  DRRWSLRGMTALVTGGTRGIGYAIVEELANFGAEVYTCSRSQNDLDECLEKWRRKGFKVS 69

Query: 66  GSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVMSTNFE 125
           G VC+V S  QR+ LME+V+S FNG LNIL+NNAG T+  K AT  T E+ S +M TNFE
Sbjct: 70  GPVCDVSSISQRQTLMESVTSSFNGKLNILINNAG-TTIPKEATNFTAEDYSIIMGTNFE 129

Query: 126 GSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLACEWAKD 185
            S+++ QLA+PLLKASGN SIVF SS AG +++P S+ YAASK AINQ+TK+LACEWAKD
Sbjct: 130 ASYNLCQLAHPLLKASGNASIVFNSSAAGVIAVPLSSIYAASKGAINQVTKSLACEWAKD 189

Query: 186 NIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAFLCLPAA 245
           +IR NAVAPW I T  +E     P   K +E ++   P+KRAGEP EVSS+V +LCLP A
Sbjct: 190 SIRVNAVAPWIINTPIIEAACQVPSQKKNIESLIGRAPMKRAGEPSEVSSLVTYLCLPTA 249

Query: 246 SYITGQLFVIDGGHTVKAF 265
           SYITGQ+  +DGG+TV  F
Sbjct: 250 SYITGQIICVDGGYTVNGF 267

[Close]

BLAST of HG10020340.1 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 4.7e-82
Identity = 155/259 (59.85%), Postives = 195/259 (75.29%), Query Frame = 0


Query: 6   NQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEMGLKVS 65
           ++RWSL G TALVTGG+RGIGRA VEELA+FGA +HTC R+QE+L  CL +W+  GL VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 66  GSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVMSTNFE 125
           GSVC+   R+QR+KL++  SS F+G LNIL+NN G T+  K   E + EE + +MSTN E
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVG-TNVRKPTVEYSSEEYAKIMSTNLE 123

Query: 126 GSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLACEWAKD 185
            +FH+SQ+A+PLLKASG GSIVFISSVAG + L   + Y A+K A+NQ+T+NLACEWA D
Sbjct: 124 SAFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASD 183

Query: 186 NIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAFLCLPAA 245
           NIR N VAPW+I+T  VE   +     + +E V+S TPL R GEP EVSS+VAFLCLPA+
Sbjct: 184 NIRTNCVAPWYIKTSLVETLLEKK---EFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPAS 243

Query: 246 SYITGQLFVIDGGHTVKAF 265
           SYITGQ+  +DGG TV  F
Sbjct: 244 SYITGQVISVDGGFTVNGF 258

[Close]

BLAST of HG10020340.1 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 1.8e-81
Identity = 155/264 (58.71%), Postives = 195/264 (73.86%), Query Frame = 0


Query: 1   MANTRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEM 60
           +A T  +RWSL GM+ALVTGG+RGIGRA VEELA  GA +HTC R++ +LE CL +W   
Sbjct: 58  IAITSKERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRS 117

Query: 61  GLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVM 120
           G +V+GSVC+V  R QR+ LMETVSS+F G L+ILVNN G T+  K   E T  E S +M
Sbjct: 118 GFRVAGSVCDVSDRSQREALMETVSSVFEGKLHILVNNVG-TNIRKPMVEFTAGEFSTLM 177

Query: 121 STNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLAC 180
           STNFE  FH+ QLAYPLL+ S  GS+VFISSV+GF+SL   +  +++K AINQ+T++LAC
Sbjct: 178 STNFESVFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLAC 237

Query: 181 EWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAFL 240
           EWAKDNIR NAVAPW+I+T  VE    +    + +E V S+TPL R GEP EVSS VAFL
Sbjct: 238 EWAKDNIRINAVAPWYIKTSMVEQVLSNK---EYLEEVYSVTPLGRLGEPREVSSAVAFL 297

Query: 241 CLPAASYITGQLFVIDGGHTVKAF 265
           CLPA+SYITGQ+  +DGG ++  F
Sbjct: 298 CLPASSYITGQILCVDGGMSINGF 317

[Close]

BLAST of HG10020340.1 vs. ExPASy Swiss-Prot
Match: Q9ZW20 (Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g29370 PE=3 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 5.8e-80
Identity = 154/261 (59.00%), Postives = 196/261 (75.10%), Query Frame = 0


Query: 8   RWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEMGLKVSGS 67
           +WSL+GMTALVTGGS+G+G+A VEELA  GA +HTC R +  L++ L EW+  GL+V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 68  VCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVMSTNFEGS 127
           VC+V SR+QR+KLMETVSSLF G L+ILV N G    LK  TE T EE S +++TN E +
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVPNVG-IGVLKPTTECTAEEFSFIIATNLEST 132

Query: 128 FHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLACEWAKDNI 187
           FH SQLA+PLLKASG+G+IV +SSVAG ++L  ++ Y A+K A+NQ+ +NLACEWA DNI
Sbjct: 133 FHFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDNI 192

Query: 188 RANAVAPWFIRTRFVEPPNDDPIHVKTM-EHVLSITPLKRAGEPHEVSSMVAFLCLPAAS 247
           RAN+V PWFI T    P   D +  K + E V S+TPL+R GE +EVSS+VAFLCLPAAS
Sbjct: 193 RANSVCPWFITT----PSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAAS 252

Query: 248 YITGQLFVIDGGHTVKAFPIP 268
           YITGQ   +DGG T+  F +P
Sbjct: 253 YITGQTICVDGGFTINGFSLP 268

[Close]

BLAST of HG10020340.1 vs. ExPASy Swiss-Prot
Match: P50162 (Tropinone reductase 1 OS=Datura stramonium OX=4076 GN=TR1 PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 5.4e-78
Identity = 150/262 (57.25%), Postives = 198/262 (75.57%), Query Frame = 0


Query: 1   MANTRNQ-RWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEE 60
           M N  N+ RWSLKG TALVTGGS+GIG A VEELA  GA ++TC R++++L++CLE W E
Sbjct: 8   MMNCNNEGRWSLKGTTALVTGGSKGIGYAIVEELAGLGARVYTCSRNEKELDECLEIWRE 67

Query: 61  MGLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGV 120
            GL V GSVC++ SR +R KLM+TV+ +F+G LNILVNNAG     K A + TE++ + +
Sbjct: 68  KGLNVEGSVCDLLSRTERDKLMQTVAHVFDGKLNILVNNAGVVIH-KEAKDFTEKDYNII 127

Query: 121 MSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLA 180
           M TNFE ++H+SQ+AYPLLKAS NG+++F+SS+AGF +LP  + Y+ASK AINQMTK+LA
Sbjct: 128 MGTNFEAAYHLSQIAYPLLKASQNGNVIFLSSIAGFSALPSVSLYSASKGAINQMTKSLA 187

Query: 181 CEWAKDNIRANAVAPWFIRTRFVEPP-NDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVA 240
           CEWAKDNIR N+VAP  I T  VE     +P   + +++ +  TP+ RAG+P EVS+++A
Sbjct: 188 CEWAKDNIRVNSVAPGVILTPLVETAIKKNPHQKEEIDNFIVKTPMGRAGKPQEVSALIA 247

Query: 241 FLCLPAASYITGQLFVIDGGHT 261
           FLC PAASYITGQ+   DGG T
Sbjct: 248 FLCFPAASYITGQIIWADGGFT 268

[Close]

BLAST of HG10020340.1 vs. ExPASy TrEMBL
Match: A0A5D3DN54 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001430 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 9.1e-129
Identity = 234/270 (86.67%), Postives = 250/270 (92.59%), Query Frame = 0


Query: 1   MANTRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEM 60
           MAN RNQRWSLKG TALVTGGSRGIGRATVEELAEFGA +HTCCRSQEDL+KCL+EWEEM
Sbjct: 1   MANIRNQRWSLKGKTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEEM 60

Query: 61  GLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRT-SSLKMATEVTEEEISGV 120
           G +VSGSVC+V+S+EQRKKLMETVSSLFNG+LNILVNNAGRT SSLK   EVTEEEIS V
Sbjct: 61  GFEVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEEISSV 120

Query: 121 MSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLA 180
           MSTNFE S H SQLAYPLLKASGNGSIVFISSV+G M+LP+STPYAASKAAINQMTKNLA
Sbjct: 121 MSTNFEASLHFSQLAYPLLKASGNGSIVFISSVSGLMALPFSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAF 240
           CEWAKDNIR NAVAPW I+TRFVE  NDDP+HVK ME +LS+TPLKRAGEPHEVSSMVAF
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRFVESSNDDPMHVKGMEQILSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAFPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKA+PI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 270

[Close]

BLAST of HG10020340.1 vs. ExPASy TrEMBL
Match: A0A1S3B5V0 (tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486361 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 9.1e-129
Identity = 234/270 (86.67%), Postives = 250/270 (92.59%), Query Frame = 0


Query: 1   MANTRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEM 60
           MAN RNQRWSLKG TALVTGGSRGIGRATVEELAEFGA +HTCCRSQEDL+KCL+EWEEM
Sbjct: 1   MANIRNQRWSLKGKTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEEM 60

Query: 61  GLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRT-SSLKMATEVTEEEISGV 120
           G +VSGSVC+V+S+EQRKKLMETVSSLFNG+LNILVNNAGRT SSLK   EVTEEEIS V
Sbjct: 61  GFEVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEEISSV 120

Query: 121 MSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLA 180
           MSTNFE S H SQLAYPLLKASGNGSIVFISSV+G M+LP+STPYAASKAAINQMTKNLA
Sbjct: 121 MSTNFEASLHFSQLAYPLLKASGNGSIVFISSVSGLMALPFSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAF 240
           CEWAKDNIR NAVAPW I+TRFVE  NDDP+HVK ME +LS+TPLKRAGEPHEVSSMVAF
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRFVESSNDDPMHVKGMEQILSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAFPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKA+PI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 270

[Close]

BLAST of HG10020340.1 vs. ExPASy TrEMBL
Match: A0A0A0LDM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733960 PE=4 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.2e-127
Identity = 231/270 (85.56%), Postives = 249/270 (92.22%), Query Frame = 0


Query: 1   MANTRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEM 60
           MAN RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGA +HTCCRSQEDL+KCL+EWE M
Sbjct: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60

Query: 61  GLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRT-SSLKMATEVTEEEISGV 120
           G KVSGSVC+V+S+EQRKKLMETVSSLFNG+LNILVNNAGRT SSLK   EVTEE+IS V
Sbjct: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120

Query: 121 MSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLA 180
           MSTNFE SFH SQLAYPLLKASGNGSIVFISSV+G  +LP+STPYAASKAAINQ+TKNLA
Sbjct: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180

Query: 181 CEWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAF 240
           CEWAKDNIR NAVAPW I+TR VE  NDDP+HVK +E +LS+TPLKRAGEPHEVSSMV F
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAFPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKA+PIPDL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270

[Close]

BLAST of HG10020340.1 vs. ExPASy TrEMBL
Match: A0A6J1I667 (tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111470275 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.4e-123
Identity = 225/269 (83.64%), Postives = 246/269 (91.45%), Query Frame = 0


Query: 1   MANT-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEE 60
           MANT R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA +HTC RSQ DL+KCL+EWEE
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGAIVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGV 120
           MGL VSGSVC+V SREQRK+LMETVSSLFNGSLNILVNNAG TS+ K + EVTEEE+S V
Sbjct: 61  MGLNVSGSVCDVHSREQRKRLMETVSSLFNGSLNILVNNAG-TSAYKSSLEVTEEELSSV 120

Query: 121 MSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLA 180
           MSTNFE SFH SQLA+PL+KASGNGSIVF+SSVAG MSLPYSTPYAASKAAINQ+T+NLA
Sbjct: 121 MSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNLA 180

Query: 181 CEWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAF 240
           CEWAKDNIR NAVAPW IRTRFV+PP DDPIHV+ M H+LS+TPLKRAGEPHEVSSMVAF
Sbjct: 181 CEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAFPIPD 269
           LCLPAASYITGQ+F IDGGHTV AFPIPD
Sbjct: 241 LCLPAASYITGQVFCIDGGHTVTAFPIPD 268

[Close]

BLAST of HG10020340.1 vs. ExPASy TrEMBL
Match: A0A6J1FKX7 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446681 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 6.3e-122
Identity = 221/269 (82.16%), Postives = 246/269 (91.45%), Query Frame = 0


Query: 1   MANT-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEE 60
           MANT R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA++HTC RSQ DL+KCL+EWEE
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGV 120
           MG  VSGSVC+V SREQR +L+ETVSSLFNGSLNILVNNAG TS+ K + EVTEEE+S V
Sbjct: 61  MGFNVSGSVCDVHSREQRMQLIETVSSLFNGSLNILVNNAG-TSAYKSSLEVTEEELSSV 120

Query: 121 MSTNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLA 180
           MSTNFE +FH SQLA+PL+KASGNGSIVF+SSVAG MSLPYSTPYAASKAAINQ+T+NLA
Sbjct: 121 MSTNFEAAFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNLA 180

Query: 181 CEWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAF 240
           CEWAKDNIR NAVAPW IRTRFV+PP DDPIHV+ M H+LS+TPLKRAGEPHEVSSMVAF
Sbjct: 181 CEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAFPIPD 269
           LCLPAASYITGQ+F IDGGHTVKAFP+PD
Sbjct: 241 LCLPAASYITGQVFCIDGGHTVKAFPVPD 268

[Close]

BLAST of HG10020340.1 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 3.4e-83
Identity = 155/259 (59.85%), Postives = 195/259 (75.29%), Query Frame = 0


Query: 6   NQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEMGLKVS 65
           ++RWSL G TALVTGG+RGIGRA VEELA+FGA +HTC R+QE+L  CL +W+  GL VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 66  GSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVMSTNFE 125
           GSVC+   R+QR+KL++  SS F+G LNIL+NN G T+  K   E + EE + +MSTN E
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVG-TNVRKPTVEYSSEEYAKIMSTNLE 123

Query: 126 GSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLACEWAKD 185
            +FH+SQ+A+PLLKASG GSIVFISSVAG + L   + Y A+K A+NQ+T+NLACEWA D
Sbjct: 124 SAFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASD 183

Query: 186 NIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAFLCLPAA 245
           NIR N VAPW+I+T  VE   +     + +E V+S TPL R GEP EVSS+VAFLCLPA+
Sbjct: 184 NIRTNCVAPWYIKTSLVETLLEKK---EFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPAS 243

Query: 246 SYITGQLFVIDGGHTVKAF 265
           SYITGQ+  +DGG TV  F
Sbjct: 244 SYITGQVISVDGGFTVNGF 258

[Close]

BLAST of HG10020340.1 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 1.3e-82
Identity = 155/264 (58.71%), Postives = 195/264 (73.86%), Query Frame = 0


Query: 1   MANTRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEM 60
           +A T  +RWSL GM+ALVTGG+RGIGRA VEELA  GA +HTC R++ +LE CL +W   
Sbjct: 58  IAITSKERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRS 117

Query: 61  GLKVSGSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVM 120
           G +V+GSVC+V  R QR+ LMETVSS+F G L+ILVNN G T+  K   E T  E S +M
Sbjct: 118 GFRVAGSVCDVSDRSQREALMETVSSVFEGKLHILVNNVG-TNIRKPMVEFTAGEFSTLM 177

Query: 121 STNFEGSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLAC 180
           STNFE  FH+ QLAYPLL+ S  GS+VFISSV+GF+SL   +  +++K AINQ+T++LAC
Sbjct: 178 STNFESVFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLAC 237

Query: 181 EWAKDNIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAFL 240
           EWAKDNIR NAVAPW+I+T  VE    +    + +E V S+TPL R GEP EVSS VAFL
Sbjct: 238 EWAKDNIRINAVAPWYIKTSMVEQVLSNK---EYLEEVYSVTPLGRLGEPREVSSAVAFL 297

Query: 241 CLPAASYITGQLFVIDGGHTVKAF 265
           CLPA+SYITGQ+  +DGG ++  F
Sbjct: 298 CLPASSYITGQILCVDGGMSINGF 317

[Close]

BLAST of HG10020340.1 vs. TAIR 10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 298.9 bits (764), Expect = 4.1e-81
Identity = 154/261 (59.00%), Postives = 196/261 (75.10%), Query Frame = 0


Query: 8   RWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEMGLKVSGS 67
           +WSL+GMTALVTGGS+G+G+A VEELA  GA +HTC R +  L++ L EW+  GL+V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 68  VCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVMSTNFEGS 127
           VC+V SR+QR+KLMETVSSLF G L+ILV N G    LK  TE T EE S +++TN E +
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVPNVG-IGVLKPTTECTAEEFSFIIATNLEST 132

Query: 128 FHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLACEWAKDNI 187
           FH SQLA+PLLKASG+G+IV +SSVAG ++L  ++ Y A+K A+NQ+ +NLACEWA DNI
Sbjct: 133 FHFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDNI 192

Query: 188 RANAVAPWFIRTRFVEPPNDDPIHVKTM-EHVLSITPLKRAGEPHEVSSMVAFLCLPAAS 247
           RAN+V PWFI T    P   D +  K + E V S+TPL+R GE +EVSS+VAFLCLPAAS
Sbjct: 193 RANSVCPWFITT----PSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAAS 252

Query: 248 YITGQLFVIDGGHTVKAFPIP 268
           YITGQ   +DGG T+  F +P
Sbjct: 253 YITGQTICVDGGFTINGFSLP 268

[Close]

BLAST of HG10020340.1 vs. TAIR 10
Match: AT2G29350.1 (senescence-associated gene 13 )

HSP 1 Score: 291.2 bits (744), Expect = 8.5e-79
Identity = 150/259 (57.92%), Postives = 186/259 (71.81%), Query Frame = 0


Query: 6   NQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEMGLKVS 65
           N RWSL GMTALVTGGS+GIG A VEELA  GA +HTC R +  L++ L EW+  G +V+
Sbjct: 10  NSRWSLGGMTALVTGGSKGIGEAVVEELAMLGAKVHTCARDETQLQERLREWQAKGFQVT 69

Query: 66  GSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVMSTNFE 125
            SVC+V SR+QR KLMETVSSL+ G LNILVNN G TS  K  TE T E+ S VM+TN E
Sbjct: 70  TSVCDVSSRDQRVKLMETVSSLYQGKLNILVNNVG-TSIFKPTTEYTAEDFSFVMATNLE 129

Query: 126 GSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLACEWAKD 185
            +FH+SQLA+PLLKASG+GSIV ISS AG + +   + Y A+K A+NQ+ +NLACEWA D
Sbjct: 130 SAFHLSQLAHPLLKASGSGSIVLISSAAGVVHVNVGSIYGATKGAMNQLARNLACEWASD 189

Query: 186 NIRANAVAPWFIRTRFVEPPNDDPIHVKTMEHVLSITPLKRAGEPHEVSSMVAFLCLPAA 245
           NIR N+V PW+I T    P ++D    +  +  +  TP+ R GE +EVS +VAFLCLP+A
Sbjct: 190 NIRTNSVCPWYITT----PLSNDFFDEEFKKEAVRTTPMGRVGEANEVSPLVAFLCLPSA 249

Query: 246 SYITGQLFVIDGGHTVKAF 265
           SYITGQ   +DGG TV  F
Sbjct: 250 SYITGQTICVDGGATVNGF 263

[Close]

BLAST of HG10020340.1 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 1.6e-77
Identity = 149/260 (57.31%), Postives = 189/260 (72.69%), Query Frame = 0


Query: 6   NQRWSLKGMTALVTGGSRGIGRATVEELAEFGASIHTCCRSQEDLEKCLEEWEEMGLKVS 65
           ++RWSL+GM ALVTGG++GIG A VEEL+  GA +HTC R +  L++ L EW+E G +V+
Sbjct: 2   DKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVT 61

Query: 66  GSVCNVESREQRKKLMETVSSLFNGSLNILVNNAGRTSSLKMATEVTEEEISGVMSTNFE 125
            S+C+V  REQR+KLMETVSSLF G LNILVNN G T  LK  TE T EE S +M+TN +
Sbjct: 62  TSICDVSLREQREKLMETVSSLFQGKLNILVNNVG-TLMLKPTTEYTAEEFSFLMATNLD 121

Query: 126 GSFHISQLAYPLLKASGNGSIVFISSVAGFMSLPYSTPYAASKAAINQMTKNLACEWAKD 185
            +FHISQLA+PLLKASG+GSIV +SS+AG + +   + Y A+K A+NQ+ +NLACEWA D
Sbjct: 122 SAFHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASD 181

Query: 186 NIRANAVAPWFIRTRFVEPPNDDPIHVKTM-EHVLSITPLKRAGEPHEVSSMVAFLCLPA 245
           NIR NA+ PW I T    P   D + V+ M +     TP+ R GE +EVS +VAFLCLPA
Sbjct: 182 NIRTNAICPWLITT----PLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPA 241

Query: 246 ASYITGQLFVIDGGHTVKAF 265
           ASYITGQ+  +DGG TV  F
Sbjct: 242 ASYITGQVICVDGGLTVNGF 256

The following BLAST results are available for this feature:

BLAST of HG10020340.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904663.1	1.3e-132	88.85	tropinone reductase homolog [Benincasa hispida]	[more]
XP_008442511.1	1.9e-128	86.67	PREDICTED: tropinone reductase homolog [Cucumis melo] >KAA0044114.1 tropinone re...	[more]
XP_004137777.1	4.6e-127	85.56	tropinone reductase homolog [Cucumis sativus] >KGN58842.1 hypothetical protein C...	[more]
XP_022971600.1	9.0e-123	83.64	tropinone reductase homolog [Cucurbita maxima]	[more]
XP_022941371.1	1.3e-121	82.16	tropinone reductase homolog [Cucurbita moschata]	[more]

BLAST of HG10020340.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50165	5.4e-86	61.78	Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1	[more]
Q9LHT0	4.7e-82	59.85	Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060...	[more]
Q9ZW12	1.8e-81	58.71	Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=...	[more]
Q9ZW20	5.8e-80	59.00	Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g293...	[more]
P50162	5.4e-78	57.25	Tropinone reductase 1 OS=Datura stramonium OX=4076 GN=TR1 PE=1 SV=1	[more]

BLAST of HG10020340.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DN54	9.1e-129	86.67	Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B5V0	9.1e-129	86.67	tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486361 PE=4 SV=1	[more]
A0A0A0LDM3	2.2e-127	85.56	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733960 PE=4 SV=1	[more]
A0A6J1I667	4.4e-123	83.64	tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111470275 PE=4 SV=...	[more]
A0A6J1FKX7	6.3e-122	82.16	tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446681 PE=4 S...	[more]

BLAST of HG10020340.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G06060.1	3.4e-83	59.85	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29260.1	1.3e-82	58.71	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29370.1	4.1e-81	59.00	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29350.1	8.5e-79	57.92	senescence-associated gene 13	[more]
AT2G29290.2	1.6e-77	57.31	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 90..101 score: 50.77 coord: 164..183 score: 40.79 coord: 144..152 score: 53.59
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 185..202 score: 37.25 coord: 90..101 score: 56.39 coord: 164..183 score: 43.35 coord: 223..243 score: 33.77 coord: 15..32 score: 50.99 coord: 138..154 score: 36.73
None	No IPR available	GENE3D	3.40.50.720		coord: 1..264 e-value: 8.4E-84 score: 282.8
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 22..260 e-value: 1.9E-56 score: 191.3
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 17..203 e-value: 8.2E-21 score: 72.3
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 10..263 e-value: 1.7E-14 score: 51.4
IPR045000	Tropinone reductase	PANTHER	PTHR42898	TROPINONE REDUCTASE	coord: 3..264
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 151..179
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 12..261

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10020340	HG10020340	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10020340.1-cds	HG10020340.1-cds-Chr04:30990408..30990484	CDS
HG10020340.1-cds	HG10020340.1-cds-Chr04:30990556..30990763	CDS
HG10020340.1-cds	HG10020340.1-cds-Chr04:30990838..30991057	CDS
HG10020340.1-cds	HG10020340.1-cds-Chr04:30991630..30991742	CDS
HG10020340.1-cds	HG10020340.1-cds-Chr04:30991849..30992040	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10020340.1	HG10020340.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity