HG10019859.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10019859.1 vs. NCBI nr
Match: XP_038903971.1 (protein NRT1/ PTR FAMILY 3.1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1066.6 bits (2757), Expect = 7.5e-308
Identity = 545/603 (90.38%), Postives = 558/603 (92.54%), Query Frame = 0

Query: 1   MEIESLRASVVDEKILRDEENDEKEEIMKMKKKKLGGVKTMPFILGNEVCDRFASSGFHA 60
           MEIESLR S VDEKILRDEE DEKEEI   KKK+LGGVKTMPFILGNEVCDRFASSGFHA
Sbjct: 1   MEIESLRVS-VDEKILRDEEKDEKEEI--KKKKQLGGVKTMPFILGNEVCDRFASSGFHA 60

Query: 61  NIITYLTQDLNMPLVPASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGM 120
           NIITYLTQDLNMPLVPASNILTNF+ATSSFTSLIGALIADSF GRFWTIT ASIIYELGM
Sbjct: 61  NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFTGRFWTITVASIIYELGM 120

Query: 121 ITITISAILPSLHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQF 180
            TITISAILPSLHPPPCPTQLNCTQASG QLM LYL+LLLTSLGAGGIRPCVVAFAADQF
Sbjct: 121 ATITISAILPSLHPPPCPTQLNCTQASGMQLMVLYLSLLLTSLGAGGIRPCVVAFAADQF 180

Query: 181 DMTKVGVAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAF 240
           DMTKVG+AGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAF
Sbjct: 181 DMTKVGIAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 240

Query: 241 VVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPHDSNLL------------------ 300
           VVGSPLYNKLKP GSPLVRLAQVVVAA KNRKA+LP+DS LL                  
Sbjct: 241 VVGSPLYNKLKPGGSPLVRLAQVVVAAVKNRKAILPNDSKLLYRNHKLDAAIAVQGRLVH 300

Query: 301 -----WLDKAAVTTTPNLITDPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
                WLDKAAV TTP+  TD PNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 301 TDQFKWLDKAAVITTPDSTTDQPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360

Query: 361 HSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTNNPSGIT 420
           HSFTIQQARTMNRHLTPTFQIPPATLSIFGI+SMLTGL+LYDRLLVPFAKKLTNNPSGIT
Sbjct: 361 HSFTIQQARTMNRHLTPTFQIPPATLSIFGIISMLTGLILYDRLLVPFAKKLTNNPSGIT 420

Query: 421 CLQRMGVGFAINILATLVSSIIEIKRKKVAANHGLLDDPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFAINILATLVSSI+EIKRKKVAA HGLLD+PTATIPFSVFWLVPQFWLHGIA
Sbjct: 421 CLQRMGVGFAINILATLVSSIVEIKRKKVAALHGLLDNPTATIPFSVFWLVPQFWLHGIA 480

Query: 481 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYVGTLMVYLVHKYTGEEHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNY+GTLMVYLVHKYTGEEHNWLPDR
Sbjct: 481 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYIGTLMVYLVHKYTGEEHNWLPDR 540

Query: 541 NLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKIIENNNGDQEGEGCEET-I 580
           NLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKIIENNNGDQE E CEET I
Sbjct: 541 NLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKIIENNNGDQEEERCEETII 600

BLAST of HG10019859.1 vs. NCBI nr
Match: NP_001267552.1 (probable nitrite transporter At1g68570-like [Cucumis sativus] >KGN59416.1 hypothetical protein Csa_000876 [Cucumis sativus] >CAA93316.2 nitrite transporter [Cucumis sativus])

HSP 1 Score: 1065.8 bits (2755), Expect = 1.3e-307
Identity = 544/605 (89.92%), Postives = 564/605 (93.22%), Query Frame = 0

Query: 1   MEIESLRASVVDEKILRDEENDEKEEI-MKMK-KKKLGGVKTMPFILGNEVCDRFASSGF 60
           MEIESLR S VDEK+L+DEE +EKEE+ MKMK K+KLGGVKTMPFILGNEVCDRFASSGF
Sbjct: 1   MEIESLRVS-VDEKVLKDEEKNEKEEMKMKMKMKRKLGGVKTMPFILGNEVCDRFASSGF 60

Query: 61  HANIITYLTQDLNMPLVPASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYEL 120
           H+NIITYLTQDLNMPLVPASNILTNF+ATSSFTSLIGALIADSFAGRFWTIT ASIIYEL
Sbjct: 61  HSNIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYEL 120

Query: 121 GMITITISAILPSLHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAAD 180
           GM+TITISAILPSLHPPPCPTQ+NCTQASGTQLM LYLALLLTSLGAGGIRPCVVAFAAD
Sbjct: 121 GMVTITISAILPSLHPPPCPTQINCTQASGTQLMILYLALLLTSLGAGGIRPCVVAFAAD 180

Query: 181 QFDMTKVGVAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVV 240
           QFDMTKVG+AGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVV
Sbjct: 181 QFDMTKVGIAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVV 240

Query: 241 AFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPHDSNLL---------------- 300
           AFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLP DS LL                
Sbjct: 241 AFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPDDSKLLYRNHELDAAIAIQGRL 300

Query: 301 -------WLDKAAVTTTPNLITDPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASS 360
                  WLDKAAV T+P+   +PPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASS
Sbjct: 301 VHTDQFKWLDKAAVITSPDSTANPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASS 360

Query: 361 HQHSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTNNPSG 420
           HQHSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLT+NPSG
Sbjct: 361 HQHSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTHNPSG 420

Query: 421 ITCLQRMGVGFAINILATLVSSIIEIKRKKVAANHGLLDDPTATIPFSVFWLVPQFWLHG 480
           ITCLQRMGVGFAINILATLVSSI+EIKRKKVAANHGLLD+PTATIPFSVFWLVPQFWLHG
Sbjct: 421 ITCLQRMGVGFAINILATLVSSIVEIKRKKVAANHGLLDNPTATIPFSVFWLVPQFWLHG 480

Query: 481 IAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYVGTLMVYLVHKYTGEEHNWLP 540
           IAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNY+GTLMVYLVHKYTGEEHNWLP
Sbjct: 481 IAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYIGTLMVYLVHKYTGEEHNWLP 540

Query: 541 DRNLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKI-IENNNGDQEGEGCEE 580
           DRNLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKI IEN +GDQE E CE+
Sbjct: 541 DRNLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKINIENKDGDQEWERCED 600

BLAST of HG10019859.1 vs. NCBI nr
Match: XP_016899751.1 (PREDICTED: protein NRT1/ PTR FAMILY 3.1-like [Cucumis melo])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.6e-289
Identity = 503/560 (89.82%), Postives = 520/560 (92.86%), Query Frame = 0

Query: 44  ILGNEVCDRFASSGFHANIITYLTQDLNMPLVPASNILTNFSATSSFTSLIGALIADSFA 103
           I+GNEVCDRFASSGFH+NIITYLTQDLNMPLVPASNILTNF+ATSSFTSLIGALIADSFA
Sbjct: 12  IVGNEVCDRFASSGFHSNIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFA 71

Query: 104 GRFWTITFASIIYELGMITITISAILPSLHPPPCPTQLNCTQASGTQLMFLYLALLLTSL 163
           GRFWTIT ASIIYELGM+TITISAILPSLHPPPCPTQ+NC QASGTQLMFLYLALLLTSL
Sbjct: 72  GRFWTITIASIIYELGMVTITISAILPSLHPPPCPTQINCIQASGTQLMFLYLALLLTSL 131

Query: 164 GAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWG 223
           GAGGIRPCVVAFAADQFDMTKVG+AGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWG
Sbjct: 132 GAGGIRPCVVAFAADQFDMTKVGIAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWG 191

Query: 224 WGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPHDSNLL- 283
           WGFG+PTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLP DS LL 
Sbjct: 192 WGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPDDSELLY 251

Query: 284 ----------------------WLDKAAVTTTPNLITDPPNLWRLATVHRVEELKSIIRM 343
                                 WLDKAAV TTP+   +PPNLWRLATVHRVEELKSIIRM
Sbjct: 252 RNHELDAAIAVQGKLVHTDQFKWLDKAAVITTPDSTANPPNLWRLATVHRVEELKSIIRM 311

Query: 344 LPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDR 403
           LPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA+LSIFGILSMLTGLVLYDR
Sbjct: 312 LPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPASLSIFGILSMLTGLVLYDR 371

Query: 404 LLVPFAKKLTNNPSGITCLQRMGVGFAINILATLVSSIIEIKRKKVAANHGLLDDPTATI 463
           LLVPFAKKLT+NP GITCLQRMGVGFAINILATLVSS +EIKRKKVAANHGLLD+PTATI
Sbjct: 372 LLVPFAKKLTHNPLGITCLQRMGVGFAINILATLVSSAVEIKRKKVAANHGLLDNPTATI 431

Query: 464 PFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYVGTLMV 523
           PFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWLAIS GNY+GTLMV
Sbjct: 432 PFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISAGNYIGTLMV 491

Query: 524 YLVHKYTGEEHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEK-I 580
           YLVHKYTGEEHNWLPDRNLNRGRLEYYYWLVSGIQV+NLVYYVICAWFYTYKPLEEEK I
Sbjct: 492 YLVHKYTGEEHNWLPDRNLNRGRLEYYYWLVSGIQVINLVYYVICAWFYTYKPLEEEKTI 551

BLAST of HG10019859.1 vs. NCBI nr
Match: XP_022971207.1 (protein NRT1/ PTR FAMILY 3.1-like [Cucurbita maxima])

HSP 1 Score: 978.0 bits (2527), Expect = 3.5e-281
Identity = 499/604 (82.62%), Postives = 531/604 (87.91%), Query Frame = 0

Query: 1   MEIESLRASVVDEKILRDEENDEKEEIMKMKKKKLGGVKTMPFILGNEVCDRFASSGFHA 60
           ME+ESL  S   EK +      EK+E+   K++KLGGVKTMPFILGNE+CDRFAS+GFHA
Sbjct: 5   MEMESLSVSADAEKGMA-----EKQEMETRKERKLGGVKTMPFILGNEICDRFASTGFHA 64

Query: 61  NIITYLTQDLNMPLVPASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGM 120
           NIITYLTQDLNMPLVPASNILTNF+ATSSFT+LIGALIADSFAGRFWTITFASIIYELGM
Sbjct: 65  NIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGM 124

Query: 121 ITITISAILPSLHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQF 180
            TITISAI+P LHPPPCPTQLNC QAS TQL   YLALLLTSLGAGGIRPCVVAFAADQF
Sbjct: 125 ATITISAIIPRLHPPPCPTQLNCIQASSTQLTVFYLALLLTSLGAGGIRPCVVAFAADQF 184

Query: 181 DMTKVGVAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAF 240
           DMTKVGVAGRTWNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAF
Sbjct: 185 DMTKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 244

Query: 241 VVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPHDSNLL------------------ 300
           VVGSPLYNKLKPSGSPLVRLAQVVVAA K RKA LP D  LL                  
Sbjct: 245 VVGSPLYNKLKPSGSPLVRLAQVVVAAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVH 304

Query: 301 -----WLDKAAVTTTPNLITDPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
                WLDKAAV TT    +DPPNLWRLATVHR+EELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 305 TDQFKWLDKAAVITTDESTSDPPNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQ 364

Query: 361 HSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTNNPSGIT 420
           HSFT+QQARTMNRHLTPTF+IPPA+LSIFGILSMLTGLVLY+RLLVPFA++ T NPSGIT
Sbjct: 365 HSFTVQQARTMNRHLTPTFEIPPASLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGIT 424

Query: 421 CLQRMGVGFAINILATLVSSIIEIKRKKVAANHGLLDDPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFAINILATLVSS+IEI+RKKVAA H LLDDPTATIPFSVFWLVPQFWLHGIA
Sbjct: 425 CLQRMGVGFAINILATLVSSLIEIRRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIA 484

Query: 481 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYVGTLMVYLVHKYTGEEHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRSTATALYWLAIS+GNYVGTLMV LVHKY+G+EHNWLPDR
Sbjct: 485 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDR 544

Query: 541 NLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKIIEN--NNGDQEGEGCEET 580
           NLNRGRLE YYWLVSGIQV+NLVYYV+CAWFYTYKPLEEEK  E   ++     +GCE+T
Sbjct: 545 NLNRGRLERYYWLVSGIQVLNLVYYVVCAWFYTYKPLEEEKDDEGEPSSSTLSKDGCEDT 602

BLAST of HG10019859.1 vs. NCBI nr
Match: KAG6596175.1 (Protein NRT1/ PTR FAMILY 3.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 974.5 bits (2518), Expect = 3.9e-280
Identity = 496/604 (82.12%), Postives = 531/604 (87.91%), Query Frame = 0

Query: 1   MEIESLRASVVDEKILRDEENDEKEEIMKMKKKKLGGVKTMPFILGNEVCDRFASSGFHA 60
           ME+ESL     D     ++   EK+E+   K++KLGGVKTMPFILGNE+CDRFAS+GFHA
Sbjct: 5   MEMESLSVGADD----AEKGMAEKQEMETKKERKLGGVKTMPFILGNEICDRFASTGFHA 64

Query: 61  NIITYLTQDLNMPLVPASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGM 120
           NIITYLTQDLNMPLVPASNILTNF+ATSSFT+LIGALIADSFAGRFWTITFASIIYELGM
Sbjct: 65  NIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGM 124

Query: 121 ITITISAILPSLHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQF 180
            T+TISAI+P LHPPPCPTQLNC QAS TQL  LYLALLLTSLGAGGIRPCVVAFAADQF
Sbjct: 125 ATVTISAIIPRLHPPPCPTQLNCIQASTTQLTVLYLALLLTSLGAGGIRPCVVAFAADQF 184

Query: 181 DMTKVGVAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAF 240
           DMTKVGVAGRTWNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAF
Sbjct: 185 DMTKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 244

Query: 241 VVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPHDSNLL------------------ 300
           VVGSPLYNKLKPSGSPLVRLAQVVVAA K RKA LP D  LL                  
Sbjct: 245 VVGSPLYNKLKPSGSPLVRLAQVVVAAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVH 304

Query: 301 -----WLDKAAVTTTPNLITDPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
                WLDKAAV TT    +DPPNLWRLATVHR+EELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 305 TDQFKWLDKAAVITTDESTSDPPNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQ 364

Query: 361 HSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTNNPSGIT 420
           HSFT+QQARTMNRHLTPTF+IPPA+LSIFGILSMLTGLVLY+RLLVPFA++ T NPSGIT
Sbjct: 365 HSFTVQQARTMNRHLTPTFEIPPASLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGIT 424

Query: 421 CLQRMGVGFAINILATLVSSIIEIKRKKVAANHGLLDDPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFAINILATLVSS+IEI+RKKVAA H LLDDPTATIPFSVFWLVPQFWLHGIA
Sbjct: 425 CLQRMGVGFAINILATLVSSLIEIRRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIA 484

Query: 481 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYVGTLMVYLVHKYTGEEHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRSTATALYWLAIS+GNYVGTLMV LVHKY+G+EHNWLPDR
Sbjct: 485 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDR 544

Query: 541 NLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKIIEN--NNGDQEGEGCEET 580
           NLNRGRLE YYWLVSGIQV+NLVYYV+CAWFYTYKPLEEEK  E   ++     +GC++T
Sbjct: 545 NLNRGRLERYYWLVSGIQVLNLVYYVVCAWFYTYKPLEEEKDDEGELSSSTLSKDGCQDT 603

BLAST of HG10019859.1 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.6e-183
Identity = 324/583 (55.57%), Postives = 422/583 (72.38%), Query Frame = 0

Query: 13  EKILRDEENDEKEEIMKMKKKKLGGVKTMPFILGNEVCDRFASSGFHANIITYLTQDLNM 72
           E+  +++ ++E++++     +  GG+ TMPFI  NE+C++ A  GFHAN+I+YLT  L++
Sbjct: 2   EEQSKNKISEEEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHL 61

Query: 73  PLVPASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSL 132
           PL  A+N LTNF+ TSS T L+GA IADSFAGRFWTITFASIIY++GM  +TISAI+P+L
Sbjct: 62  PLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTL 121

Query: 133 HPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTW 192
            PPPC  +  C  A   QL  LY+ALLL +LG+GGIRPCVVAF ADQFD +      +TW
Sbjct: 122 RPPPCKGEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTW 181

Query: 193 NFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKP 252
           N+FNWYYFCMG A L A+TV+V+IQDNVGWG G GIPT+AM LSV+AFV G  LY  L P
Sbjct: 182 NYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVP 241

Query: 253 SGSPLVRLAQVVVAAFKNRKAVLPHDSNLLW-----------------------LDKAAV 312
           +GSP  RL QV VAAF+ RK  +  D +LL+                       LDKAA+
Sbjct: 242 AGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAI 301

Query: 313 TTTPNLIT--DPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQART 372
            T  + +     PN WRL+TVHRVEELKS+IRM PI A+GILL+TA + Q +F++QQA+T
Sbjct: 302 VTEEDNLKPGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKT 361

Query: 373 MNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFA 432
           MNRHLT +FQIP  ++S+F  ++MLT ++ YDR+ V  A+K T    GIT L RMG+GF 
Sbjct: 362 MNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFV 421

Query: 433 INILATLVSSIIEIKRKKVAANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLE 492
           I+I+ATLV+  +E+KRK VA  HGLLD P   +P S  WL+PQ+ LHG+AE FMS+GHLE
Sbjct: 422 ISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLE 481

Query: 493 FMYDQSPESLRSTATALYWLAISVGNYVGTLMVYLVHKYTG--EEHNWLPDRNLNRGRLE 552
           F YDQ+PES+RSTATAL+W+AIS+GNYV TL+V LVHK++   +  NWLPD NLNRGRLE
Sbjct: 482 FFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLE 541

Query: 553 YYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKIIENNNGDQE 569
           Y+YWL++ +Q +NLVYY+ CA  YTYKP++     E+++  +E
Sbjct: 542 YFYWLITVLQAVNLVYYLWCAKIYTYKPVQVHHSKEDSSPVKE 584

BLAST of HG10019859.1 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 4.7e-119
Identity = 235/547 (42.96%), Postives = 333/547 (60.88%), Query Frame = 0

Query: 32  KKKLGGVKTMPFILGNEVCDRFASSGFHANIITYLTQDLNMPLVPASNILTNFSATSSFT 91
           K K G  K   FILG E C+R A  G   N+I YL + +NM  V AS  ++N+S T   T
Sbjct: 23  KNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSNWSGTCYAT 82

Query: 92  SLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPPPCPTQLNCTQASGTQL 151
            LIGA IAD++ GR+WTI    +IY  GM  +TISA +P L  P C  +  C   +G Q 
Sbjct: 83  PLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTCSGE-TCHATAG-QT 142

Query: 152 MFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGMATLTALT 211
              ++AL L +LG GGI+PCV +F ADQFD T         +FFNW+YF + +  + A +
Sbjct: 143 AITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVINVGAMIASS 202

Query: 212 VVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNR 271
           V+V+IQ NVGWGWG G+PT+AMA++VV F  GS  Y   KP GSPL R+ QV+VA+ +  
Sbjct: 203 VLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVASCRKS 262

Query: 272 KAVLPHDSNLLW-----------------------LDKAAV-TTTPNLITDPPNLWRLAT 331
           K  +P D +LL+                        DKAAV T + N      + W+L T
Sbjct: 263 KVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNKGAAKSSSWKLCT 322

Query: 332 VHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPATLSIFG 391
           V +VEELK++IR+LPIWA GI+  +  S   +  + Q  T+++H+ P F+IP A+LS+F 
Sbjct: 323 VTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSASLSLFD 382

Query: 392 ILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFAINILATLVSSIIEIKRKKVA 451
            LS+L    +YD+L+VPFA+K T +  G T LQR+G+G  I+I + + + I+E+ R    
Sbjct: 383 TLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEVARLNYV 442

Query: 452 ANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWL 511
             H L ++   TIP ++FW VPQ++L G AEVF  +G LEF YDQ+P+++RS  +AL   
Sbjct: 443 QTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLT 502

Query: 512 AISVGNYVGTLMVYLVHKYT--GEEHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVYYVIC 553
           AI+ GNY+ T +V LV K T  G    W+  +NLN G L+Y++WL++G+  +N + Y+  
Sbjct: 503 AIAFGNYLSTFLVTLVTKVTRSGGRPGWIA-KNLNNGHLDYFFWLLAGLSFLNFLVYLWI 562

BLAST of HG10019859.1 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 3.1e-115
Identity = 223/547 (40.77%), Postives = 322/547 (58.87%), Query Frame = 0

Query: 32  KKKLGGVKTMPFILGNEVCDRFASSGFHANIITYLTQDLNMPLVPASNILTNFSATSSFT 91
           K+K G  K   FILGNE C+R A  G   N++ YL   LN     A+N +TN+S T   T
Sbjct: 22  KEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSGTCYIT 81

Query: 92  SLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPPPCPTQLNCTQASGTQL 151
            LIGA IAD++ GR+WTI     IY  GM  +T+SA +P L P  C    +    + +Q 
Sbjct: 82  PLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNC--NADTCHPNSSQT 141

Query: 152 MFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGMATLTALT 211
              ++AL + +LG GGI+PCV +F ADQFD        +  +FFNW+YF + +  L A T
Sbjct: 142 AVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGALIAAT 201

Query: 212 VVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNR 271
           V+V+IQ NVGWGWGFG+PT+AM ++V  F  GS  Y   +P GSPL R+ QV+VAAF+  
Sbjct: 202 VLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKI 261

Query: 272 KAVLP-----------------------HDSNLLWLDKAAVTTTPNLITD-PPNLWRLAT 331
              +P                       H  NL + DKAAV +  + I D   N WRL +
Sbjct: 262 SVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGEVNPWRLCS 321

Query: 332 VHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPATLSIFG 391
           V +VEELKSII +LP+WA GI+  T  S   +  + Q  TM++H+   F+IP A+LS+F 
Sbjct: 322 VTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSASLSLFD 381

Query: 392 ILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFAINILATLVSSIIEIKRKKVA 451
            +S+L    +YD+ ++P A+K T N  G T LQRMG+G  ++I A + + ++E+ R    
Sbjct: 382 TVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVVRLDYV 441

Query: 452 ANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWL 511
             H   D     I  S+FW +PQ+ L G AEVF  +G LEF YDQ+P+++RS  +AL   
Sbjct: 442 KTHNAYDQ--KQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLT 501

Query: 512 AISVGNYVGTLMVYLVHKYTGE--EHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVYYVIC 553
            +++GNY+ T++V +V K T +  +  W+PD NLNRG L+Y+++L++ +  +N + Y+  
Sbjct: 502 TVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGHLDYFFYLLATLSFLNFLVYLWI 561

BLAST of HG10019859.1 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.6e-114
Identity = 227/547 (41.50%), Postives = 329/547 (60.15%), Query Frame = 0

Query: 32  KKKLGGVKTMPFILGNEVCDRFASSGFHANIITYLTQDLNMPLVPASNILTNFSATSSFT 91
           K+K G  K  PFILGNE C+R A  G   N+ITYLT  L+   V A+  +T +  T   T
Sbjct: 39  KEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLT 98

Query: 92  SLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPPPCPTQLNCTQASGTQL 151
            LIGA++AD++ GR+WTI   S IY +GM  +T+SA +P+L P  C     C  A+  Q 
Sbjct: 99  PLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECIGDF-CPSATPAQY 158

Query: 152 MFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGMATLTALT 211
              +  L L +LG GGI+PCV +F ADQFD T      R  +FFNW+YF + +  L + +
Sbjct: 159 AMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSSS 218

Query: 212 VVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNR 271
           ++V+IQ+N GWG GFGIPT+ M L++ +F  G+PLY   KP GSP+ R++QVVVA+F+  
Sbjct: 219 LLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRKS 278

Query: 272 KAVLPHDSNLL-----------------------WLDKAAVTTTPNLIT-DPPNLWRLAT 331
              +P D+ LL                       +LDKAAV +     + D  N WRL T
Sbjct: 279 SVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEEESKSGDYSNSWRLCT 338

Query: 332 VHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPATLSIFG 391
           V +VEELK +IRM PIWA+GI+     +   +  +QQ R MN  +  +FQ+PPA L  F 
Sbjct: 339 VTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKI-GSFQLPPAALGTFD 398

Query: 392 ILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFAINILATLVSSIIEIKRKKVA 451
             S++  + LYDR +VP A+K T    G T +QRMG+G  +++L    ++I+EI R  +A
Sbjct: 399 TASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMA 458

Query: 452 ANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWL 511
            + GL++   A +P SV W +PQ+++ G AEVF  +G LEF YDQSP+++RS  +AL  L
Sbjct: 459 NDLGLVES-GAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALL 518

Query: 512 AISVGNYVGTLMVYLVHKYT--GEEHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVYYVIC 553
             ++GNY+ +L++ LV  +T    +  W+ D NLN G L+Y++WL++G+ ++N+  Y   
Sbjct: 519 TNALGNYLSSLILTLVTYFTTRNGQEGWISD-NLNSGHLDYFFWLLAGLSLVNMAVYFFS 578

BLAST of HG10019859.1 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.5e-112
Identity = 237/558 (42.47%), Postives = 337/558 (60.39%), Query Frame = 0

Query: 32  KKKLGGVKTMPFILGNEVCDRFASSGFHANIITYLTQDLNMPLVPASNILTNFSATSSFT 91
           +KK GG + + FILGNE  +R  S G  AN + YLT+  ++  V A+N++  +S  ++ T
Sbjct: 49  EKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLT 108

Query: 92  SLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPPPCPTQ--LNCTQASGT 151
            L+GA I+D++ GRF TI FAS    LG+ITIT++A  P LHP  C +Q  L+C   +  
Sbjct: 109 PLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKL 168

Query: 152 QLMFLYLALLLTSLGAGGIRPCVVAFAADQFDM-TKVGVAGRTWNFFNWYYFCMGMATLT 211
           Q+  L L L   S+G+GGIRPC + F  DQFD  T+ GV G   +FFNWYY    +  + 
Sbjct: 169 QIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKG-VASFFNWYYMTFTVVLII 228

Query: 212 ALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAF 271
             TVVVYIQD V W  GF IPT  MAL+VV F  G   Y  +KP GS    +AQV+VAA 
Sbjct: 229 TQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAAR 288

Query: 272 KNRKAVLP----------------------HDSN-LLWLDKAAVTTTPNLITD--PPNLW 331
           K RK  LP                      H SN    LDKAAV    +L  +  P + W
Sbjct: 289 KKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEGDLTPEGPPADKW 348

Query: 332 RLATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPATL 391
           RL +V  VEE+K +IR++PIW+AGI+ + A + Q +FT+ QA  M+R+L P F+IP  +L
Sbjct: 349 RLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSL 408

Query: 392 SIFGILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFAINILATLVSSIIEIKR 451
           S+  +L++   L  YDR+ VPF +++T + SGIT LQR+G G    I + +V+ I+E  R
Sbjct: 409 SVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMR 468

Query: 452 KKVAANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATA 511
           +  + N G   DPT   P SVFWL PQ  L G+ E F  +G +EF   Q PE +RS A +
Sbjct: 469 RIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANS 528

Query: 512 LYWLAISVGNYVGTLMVYLVHKYTG--EEHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVY 557
           L+ L+ +  +Y+ + +V +VHK++G  +  +WL ++NLN G+L+Y+Y+L++ + V+NLVY
Sbjct: 529 LFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWL-NKNLNAGKLDYFYYLIAVLGVVNLVY 588

BLAST of HG10019859.1 vs. ExPASy TrEMBL
Match: Q96400 (Nitrite transporter OS=Cucumis sativus OX=3659 GN=NiTR1 PE=2 SV=2)

HSP 1 Score: 1065.8 bits (2755), Expect = 6.2e-308
Identity = 544/605 (89.92%), Postives = 564/605 (93.22%), Query Frame = 0

Query: 1   MEIESLRASVVDEKILRDEENDEKEEI-MKMK-KKKLGGVKTMPFILGNEVCDRFASSGF 60
           MEIESLR S VDEK+L+DEE +EKEE+ MKMK K+KLGGVKTMPFILGNEVCDRFASSGF
Sbjct: 1   MEIESLRVS-VDEKVLKDEEKNEKEEMKMKMKMKRKLGGVKTMPFILGNEVCDRFASSGF 60

Query: 61  HANIITYLTQDLNMPLVPASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYEL 120
           H+NIITYLTQDLNMPLVPASNILTNF+ATSSFTSLIGALIADSFAGRFWTIT ASIIYEL
Sbjct: 61  HSNIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYEL 120

Query: 121 GMITITISAILPSLHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAAD 180
           GM+TITISAILPSLHPPPCPTQ+NCTQASGTQLM LYLALLLTSLGAGGIRPCVVAFAAD
Sbjct: 121 GMVTITISAILPSLHPPPCPTQINCTQASGTQLMILYLALLLTSLGAGGIRPCVVAFAAD 180

Query: 181 QFDMTKVGVAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVV 240
           QFDMTKVG+AGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVV
Sbjct: 181 QFDMTKVGIAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVV 240

Query: 241 AFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPHDSNLL---------------- 300
           AFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLP DS LL                
Sbjct: 241 AFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPDDSKLLYRNHELDAAIAIQGRL 300

Query: 301 -------WLDKAAVTTTPNLITDPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASS 360
                  WLDKAAV T+P+   +PPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASS
Sbjct: 301 VHTDQFKWLDKAAVITSPDSTANPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASS 360

Query: 361 HQHSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTNNPSG 420
           HQHSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLT+NPSG
Sbjct: 361 HQHSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTHNPSG 420

Query: 421 ITCLQRMGVGFAINILATLVSSIIEIKRKKVAANHGLLDDPTATIPFSVFWLVPQFWLHG 480
           ITCLQRMGVGFAINILATLVSSI+EIKRKKVAANHGLLD+PTATIPFSVFWLVPQFWLHG
Sbjct: 421 ITCLQRMGVGFAINILATLVSSIVEIKRKKVAANHGLLDNPTATIPFSVFWLVPQFWLHG 480

Query: 481 IAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYVGTLMVYLVHKYTGEEHNWLP 540
           IAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNY+GTLMVYLVHKYTGEEHNWLP
Sbjct: 481 IAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYIGTLMVYLVHKYTGEEHNWLP 540

Query: 541 DRNLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKI-IENNNGDQEGEGCEE 580
           DRNLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKI IEN +GDQE E CE+
Sbjct: 541 DRNLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKINIENKDGDQEWERCED 600

BLAST of HG10019859.1 vs. ExPASy TrEMBL
Match: A0A1S4DUU1 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo OX=3656 GN=LOC103487163 PE=3 SV=1)

HSP 1 Score: 1004.2 bits (2595), Expect = 2.2e-289
Identity = 503/560 (89.82%), Postives = 520/560 (92.86%), Query Frame = 0

Query: 44  ILGNEVCDRFASSGFHANIITYLTQDLNMPLVPASNILTNFSATSSFTSLIGALIADSFA 103
           I+GNEVCDRFASSGFH+NIITYLTQDLNMPLVPASNILTNF+ATSSFTSLIGALIADSFA
Sbjct: 12  IVGNEVCDRFASSGFHSNIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFA 71

Query: 104 GRFWTITFASIIYELGMITITISAILPSLHPPPCPTQLNCTQASGTQLMFLYLALLLTSL 163
           GRFWTIT ASIIYELGM+TITISAILPSLHPPPCPTQ+NC QASGTQLMFLYLALLLTSL
Sbjct: 72  GRFWTITIASIIYELGMVTITISAILPSLHPPPCPTQINCIQASGTQLMFLYLALLLTSL 131

Query: 164 GAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWG 223
           GAGGIRPCVVAFAADQFDMTKVG+AGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWG
Sbjct: 132 GAGGIRPCVVAFAADQFDMTKVGIAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWG 191

Query: 224 WGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPHDSNLL- 283
           WGFG+PTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLP DS LL 
Sbjct: 192 WGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPDDSELLY 251

Query: 284 ----------------------WLDKAAVTTTPNLITDPPNLWRLATVHRVEELKSIIRM 343
                                 WLDKAAV TTP+   +PPNLWRLATVHRVEELKSIIRM
Sbjct: 252 RNHELDAAIAVQGKLVHTDQFKWLDKAAVITTPDSTANPPNLWRLATVHRVEELKSIIRM 311

Query: 344 LPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDR 403
           LPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA+LSIFGILSMLTGLVLYDR
Sbjct: 312 LPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPASLSIFGILSMLTGLVLYDR 371

Query: 404 LLVPFAKKLTNNPSGITCLQRMGVGFAINILATLVSSIIEIKRKKVAANHGLLDDPTATI 463
           LLVPFAKKLT+NP GITCLQRMGVGFAINILATLVSS +EIKRKKVAANHGLLD+PTATI
Sbjct: 372 LLVPFAKKLTHNPLGITCLQRMGVGFAINILATLVSSAVEIKRKKVAANHGLLDNPTATI 431

Query: 464 PFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYVGTLMV 523
           PFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWLAIS GNY+GTLMV
Sbjct: 432 PFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISAGNYIGTLMV 491

Query: 524 YLVHKYTGEEHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEK-I 580
           YLVHKYTGEEHNWLPDRNLNRGRLEYYYWLVSGIQV+NLVYYVICAWFYTYKPLEEEK I
Sbjct: 492 YLVHKYTGEEHNWLPDRNLNRGRLEYYYWLVSGIQVINLVYYVICAWFYTYKPLEEEKTI 551

BLAST of HG10019859.1 vs. ExPASy TrEMBL
Match: A0A6J1I1C5 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita maxima OX=3661 GN=LOC111469999 PE=3 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 1.7e-281
Identity = 499/604 (82.62%), Postives = 531/604 (87.91%), Query Frame = 0

Query: 1   MEIESLRASVVDEKILRDEENDEKEEIMKMKKKKLGGVKTMPFILGNEVCDRFASSGFHA 60
           ME+ESL  S   EK +      EK+E+   K++KLGGVKTMPFILGNE+CDRFAS+GFHA
Sbjct: 5   MEMESLSVSADAEKGMA-----EKQEMETRKERKLGGVKTMPFILGNEICDRFASTGFHA 64

Query: 61  NIITYLTQDLNMPLVPASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGM 120
           NIITYLTQDLNMPLVPASNILTNF+ATSSFT+LIGALIADSFAGRFWTITFASIIYELGM
Sbjct: 65  NIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGM 124

Query: 121 ITITISAILPSLHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQF 180
            TITISAI+P LHPPPCPTQLNC QAS TQL   YLALLLTSLGAGGIRPCVVAFAADQF
Sbjct: 125 ATITISAIIPRLHPPPCPTQLNCIQASSTQLTVFYLALLLTSLGAGGIRPCVVAFAADQF 184

Query: 181 DMTKVGVAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAF 240
           DMTKVGVAGRTWNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAF
Sbjct: 185 DMTKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 244

Query: 241 VVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPHDSNLL------------------ 300
           VVGSPLYNKLKPSGSPLVRLAQVVVAA K RKA LP D  LL                  
Sbjct: 245 VVGSPLYNKLKPSGSPLVRLAQVVVAAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVH 304

Query: 301 -----WLDKAAVTTTPNLITDPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
                WLDKAAV TT    +DPPNLWRLATVHR+EELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 305 TDQFKWLDKAAVITTDESTSDPPNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQ 364

Query: 361 HSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTNNPSGIT 420
           HSFT+QQARTMNRHLTPTF+IPPA+LSIFGILSMLTGLVLY+RLLVPFA++ T NPSGIT
Sbjct: 365 HSFTVQQARTMNRHLTPTFEIPPASLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGIT 424

Query: 421 CLQRMGVGFAINILATLVSSIIEIKRKKVAANHGLLDDPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFAINILATLVSS+IEI+RKKVAA H LLDDPTATIPFSVFWLVPQFWLHGIA
Sbjct: 425 CLQRMGVGFAINILATLVSSLIEIRRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIA 484

Query: 481 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYVGTLMVYLVHKYTGEEHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRSTATALYWLAIS+GNYVGTLMV LVHKY+G+EHNWLPDR
Sbjct: 485 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDR 544

Query: 541 NLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKIIEN--NNGDQEGEGCEET 580
           NLNRGRLE YYWLVSGIQV+NLVYYV+CAWFYTYKPLEEEK  E   ++     +GCE+T
Sbjct: 545 NLNRGRLERYYWLVSGIQVLNLVYYVVCAWFYTYKPLEEEKDDEGEPSSSTLSKDGCEDT 602

BLAST of HG10019859.1 vs. ExPASy TrEMBL
Match: A0A6J1FLW3 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita moschata OX=3662 GN=LOC111446603 PE=3 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.6e-279
Identity = 493/599 (82.30%), Postives = 524/599 (87.48%), Query Frame = 0

Query: 1   MEIESLRASVVDEKILRDEENDEKEEIMKMKKKKLGGVKTMPFILGNEVCDRFASSGFHA 60
           ME  S+RA   ++ +       EK+E+   K++KLGGVKTMPFILGNE+CDRFAS+GFHA
Sbjct: 7   MESSSVRADDAEKGMA------EKQEMETKKERKLGGVKTMPFILGNEICDRFASTGFHA 66

Query: 61  NIITYLTQDLNMPLVPASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGM 120
           NIITYLTQDLNMPLVPASNILTNF+ATSSFT+LIGALIADSFAGRFWTITFASIIYELGM
Sbjct: 67  NIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGM 126

Query: 121 ITITISAILPSLHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQF 180
            TITISAI+P LHPPPCPTQLNC QAS TQL  LYLALLLTSLGAGGIRPCVVAFAADQF
Sbjct: 127 ATITISAIIPRLHPPPCPTQLNCIQASTTQLTVLYLALLLTSLGAGGIRPCVVAFAADQF 186

Query: 181 DMTKVGVAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAF 240
           DMTKVGVAGRTWNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAF
Sbjct: 187 DMTKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 246

Query: 241 VVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPHDSNLL------------------ 300
           VVGSPLYNKLKPSGSPLVRLAQVVV A K RKA LP D  LL                  
Sbjct: 247 VVGSPLYNKLKPSGSPLVRLAQVVVTAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVH 306

Query: 301 -----WLDKAAVTTTPNLITDPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
                WLDKAAV TT    +DPPNLWRLATVHR+EELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 307 TNQFKWLDKAAVITTDESTSDPPNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQ 366

Query: 361 HSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTNNPSGIT 420
           HSFT+QQARTMNRHLTPTF+IPPA+LSIFGILSMLTGLVLY+RLLVPFA++ T NPSGIT
Sbjct: 367 HSFTVQQARTMNRHLTPTFEIPPASLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGIT 426

Query: 421 CLQRMGVGFAINILATLVSSIIEIKRKKVAANHGLLDDPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFAINILATLVSS+IEI+RKKVAA H LLDDPTATIPFSVFWLVPQFWLHGIA
Sbjct: 427 CLQRMGVGFAINILATLVSSLIEIRRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIA 486

Query: 481 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYVGTLMVYLVHKYTGEEHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRSTATALYWLAIS+GNYVGTLMV LVHKY+G+EHNWLPDR
Sbjct: 487 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDR 546

Query: 541 NLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKIIENNNGDQEGEGCEETI 577
           NLNRGRLE YYWLVSGIQV+NLVYYV+CAWFYTYKPLEEEK       D EGE    T+
Sbjct: 547 NLNRGRLERYYWLVSGIQVLNLVYYVVCAWFYTYKPLEEEK-------DDEGEPSSSTL 592

BLAST of HG10019859.1 vs. ExPASy TrEMBL
Match: A0A5A7UI29 (Protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006470 PE=3 SV=1)

HSP 1 Score: 948.7 bits (2451), Expect = 1.1e-272
Identity = 477/532 (89.66%), Postives = 492/532 (92.48%), Query Frame = 0

Query: 72  MPLVPASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPS 131
           MPLVPASNILTNF+ATSSFTSLIGALIADSFAGRFWTIT ASIIYELGM+TITISAILPS
Sbjct: 1   MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYELGMVTITISAILPS 60

Query: 132 LHPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRT 191
           LHPPPCPTQ+NC QASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVG+AGRT
Sbjct: 61  LHPPPCPTQINCIQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRT 120

Query: 192 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLK 251
           WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFG+PTIAMALSVVAFVVGSPLYNKLK
Sbjct: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 180

Query: 252 PSGSPLVRLAQVVVAAFKNRKAVLPHDSNLL-----------------------WLDKAA 311
           PSGSPLVRLAQVVVAAFKNRKAVLP DS LL                       WLDKAA
Sbjct: 181 PSGSPLVRLAQVVVAAFKNRKAVLPDDSELLYRNHELDAAIAVQGKLVHTDQFKWLDKAA 240

Query: 312 VTTTPNLITDPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTM 371
           V TTP+   +PPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTM
Sbjct: 241 VITTPDSTANPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTM 300

Query: 372 NRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFAI 431
           NRHLTPTFQIPPA+LSIFGILSMLTGLVLYDRLLVPFAKKLT+NP GITCLQRMGVGFAI
Sbjct: 301 NRHLTPTFQIPPASLSIFGILSMLTGLVLYDRLLVPFAKKLTHNPLGITCLQRMGVGFAI 360

Query: 432 NILATLVSSIIEIKRKKVAANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEF 491
           NILATLVSS +EIKRKKVAANHGLLD+PTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEF
Sbjct: 361 NILATLVSSAVEIKRKKVAANHGLLDNPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEF 420

Query: 492 MYDQSPESLRSTATALYWLAISVGNYVGTLMVYLVHKYTGEEHNWLPDRNLNRGRLEYYY 551
           MYDQSPESLRSTATALYWLAIS GNY+GTLMVYLVHKYTGEEHNWLPDRNLNRGRLEYYY
Sbjct: 421 MYDQSPESLRSTATALYWLAISAGNYIGTLMVYLVHKYTGEEHNWLPDRNLNRGRLEYYY 480

Query: 552 WLVSGIQVMNLVYYVICAWFYTYKPLEEEK-IIENNNGDQEGEGCEETIKTA 580
           WLVSGIQV+NLVYYVICAWFYTYKPLEEEK IIEN + DQEGE CE+T K A
Sbjct: 481 WLVSGIQVINLVYYVICAWFYTYKPLEEEKTIIENKDSDQEGERCEDTSKIA 532

BLAST of HG10019859.1 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 644.0 bits (1660), Expect = 1.1e-184
Identity = 324/583 (55.57%), Postives = 422/583 (72.38%), Query Frame = 0

Query: 13  EKILRDEENDEKEEIMKMKKKKLGGVKTMPFILGNEVCDRFASSGFHANIITYLTQDLNM 72
           E+  +++ ++E++++     +  GG+ TMPFI  NE+C++ A  GFHAN+I+YLT  L++
Sbjct: 2   EEQSKNKISEEEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHL 61

Query: 73  PLVPASNILTNFSATSSFTSLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSL 132
           PL  A+N LTNF+ TSS T L+GA IADSFAGRFWTITFASIIY++GM  +TISAI+P+L
Sbjct: 62  PLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTL 121

Query: 133 HPPPCPTQLNCTQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTW 192
            PPPC  +  C  A   QL  LY+ALLL +LG+GGIRPCVVAF ADQFD +      +TW
Sbjct: 122 RPPPCKGEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTW 181

Query: 193 NFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKP 252
           N+FNWYYFCMG A L A+TV+V+IQDNVGWG G GIPT+AM LSV+AFV G  LY  L P
Sbjct: 182 NYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVP 241

Query: 253 SGSPLVRLAQVVVAAFKNRKAVLPHDSNLLW-----------------------LDKAAV 312
           +GSP  RL QV VAAF+ RK  +  D +LL+                       LDKAA+
Sbjct: 242 AGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAI 301

Query: 313 TTTPNLIT--DPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQART 372
            T  + +     PN WRL+TVHRVEELKS+IRM PI A+GILL+TA + Q +F++QQA+T
Sbjct: 302 VTEEDNLKPGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKT 361

Query: 373 MNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFA 432
           MNRHLT +FQIP  ++S+F  ++MLT ++ YDR+ V  A+K T    GIT L RMG+GF 
Sbjct: 362 MNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFV 421

Query: 433 INILATLVSSIIEIKRKKVAANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLE 492
           I+I+ATLV+  +E+KRK VA  HGLLD P   +P S  WL+PQ+ LHG+AE FMS+GHLE
Sbjct: 422 ISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLE 481

Query: 493 FMYDQSPESLRSTATALYWLAISVGNYVGTLMVYLVHKYTG--EEHNWLPDRNLNRGRLE 552
           F YDQ+PES+RSTATAL+W+AIS+GNYV TL+V LVHK++   +  NWLPD NLNRGRLE
Sbjct: 482 FFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLE 541

Query: 553 YYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKIIENNNGDQE 569
           Y+YWL++ +Q +NLVYY+ CA  YTYKP++     E+++  +E
Sbjct: 542 YFYWLITVLQAVNLVYYLWCAKIYTYKPVQVHHSKEDSSPVKE 584

BLAST of HG10019859.1 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 429.9 bits (1104), Expect = 3.3e-120
Identity = 235/547 (42.96%), Postives = 333/547 (60.88%), Query Frame = 0

Query: 32  KKKLGGVKTMPFILGNEVCDRFASSGFHANIITYLTQDLNMPLVPASNILTNFSATSSFT 91
           K K G  K   FILG E C+R A  G   N+I YL + +NM  V AS  ++N+S T   T
Sbjct: 23  KNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSNWSGTCYAT 82

Query: 92  SLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPPPCPTQLNCTQASGTQL 151
            LIGA IAD++ GR+WTI    +IY  GM  +TISA +P L  P C  +  C   +G Q 
Sbjct: 83  PLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTCSGE-TCHATAG-QT 142

Query: 152 MFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGMATLTALT 211
              ++AL L +LG GGI+PCV +F ADQFD T         +FFNW+YF + +  + A +
Sbjct: 143 AITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVINVGAMIASS 202

Query: 212 VVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNR 271
           V+V+IQ NVGWGWG G+PT+AMA++VV F  GS  Y   KP GSPL R+ QV+VA+ +  
Sbjct: 203 VLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVASCRKS 262

Query: 272 KAVLPHDSNLLW-----------------------LDKAAV-TTTPNLITDPPNLWRLAT 331
           K  +P D +LL+                        DKAAV T + N      + W+L T
Sbjct: 263 KVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNKGAAKSSSWKLCT 322

Query: 332 VHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPATLSIFG 391
           V +VEELK++IR+LPIWA GI+  +  S   +  + Q  T+++H+ P F+IP A+LS+F 
Sbjct: 323 VTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSASLSLFD 382

Query: 392 ILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFAINILATLVSSIIEIKRKKVA 451
            LS+L    +YD+L+VPFA+K T +  G T LQR+G+G  I+I + + + I+E+ R    
Sbjct: 383 TLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEVARLNYV 442

Query: 452 ANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWL 511
             H L ++   TIP ++FW VPQ++L G AEVF  +G LEF YDQ+P+++RS  +AL   
Sbjct: 443 QTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLT 502

Query: 512 AISVGNYVGTLMVYLVHKYT--GEEHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVYYVIC 553
           AI+ GNY+ T +V LV K T  G    W+  +NLN G L+Y++WL++G+  +N + Y+  
Sbjct: 503 AIAFGNYLSTFLVTLVTKVTRSGGRPGWIA-KNLNNGHLDYFFWLLAGLSFLNFLVYLWI 562

BLAST of HG10019859.1 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 417.2 bits (1071), Expect = 2.2e-116
Identity = 223/547 (40.77%), Postives = 322/547 (58.87%), Query Frame = 0

Query: 32  KKKLGGVKTMPFILGNEVCDRFASSGFHANIITYLTQDLNMPLVPASNILTNFSATSSFT 91
           K+K G  K   FILGNE C+R A  G   N++ YL   LN     A+N +TN+S T   T
Sbjct: 22  KEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSGTCYIT 81

Query: 92  SLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPPPCPTQLNCTQASGTQL 151
            LIGA IAD++ GR+WTI     IY  GM  +T+SA +P L P  C    +    + +Q 
Sbjct: 82  PLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNC--NADTCHPNSSQT 141

Query: 152 MFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGMATLTALT 211
              ++AL + +LG GGI+PCV +F ADQFD        +  +FFNW+YF + +  L A T
Sbjct: 142 AVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGALIAAT 201

Query: 212 VVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNR 271
           V+V+IQ NVGWGWGFG+PT+AM ++V  F  GS  Y   +P GSPL R+ QV+VAAF+  
Sbjct: 202 VLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKI 261

Query: 272 KAVLP-----------------------HDSNLLWLDKAAVTTTPNLITD-PPNLWRLAT 331
              +P                       H  NL + DKAAV +  + I D   N WRL +
Sbjct: 262 SVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGEVNPWRLCS 321

Query: 332 VHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPATLSIFG 391
           V +VEELKSII +LP+WA GI+  T  S   +  + Q  TM++H+   F+IP A+LS+F 
Sbjct: 322 VTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSASLSLFD 381

Query: 392 ILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFAINILATLVSSIIEIKRKKVA 451
            +S+L    +YD+ ++P A+K T N  G T LQRMG+G  ++I A + + ++E+ R    
Sbjct: 382 TVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVVRLDYV 441

Query: 452 ANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWL 511
             H   D     I  S+FW +PQ+ L G AEVF  +G LEF YDQ+P+++RS  +AL   
Sbjct: 442 KTHNAYDQ--KQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLT 501

Query: 512 AISVGNYVGTLMVYLVHKYTGE--EHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVYYVIC 553
            +++GNY+ T++V +V K T +  +  W+PD NLNRG L+Y+++L++ +  +N + Y+  
Sbjct: 502 TVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGHLDYFFYLLATLSFLNFLVYLWI 561

BLAST of HG10019859.1 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 414.1 bits (1063), Expect = 1.9e-115
Identity = 227/547 (41.50%), Postives = 329/547 (60.15%), Query Frame = 0

Query: 32  KKKLGGVKTMPFILGNEVCDRFASSGFHANIITYLTQDLNMPLVPASNILTNFSATSSFT 91
           K+K G  K  PFILGNE C+R A  G   N+ITYLT  L+   V A+  +T +  T   T
Sbjct: 39  KEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLT 98

Query: 92  SLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPPPCPTQLNCTQASGTQL 151
            LIGA++AD++ GR+WTI   S IY +GM  +T+SA +P+L P  C     C  A+  Q 
Sbjct: 99  PLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECIGDF-CPSATPAQY 158

Query: 152 MFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGMATLTALT 211
              +  L L +LG GGI+PCV +F ADQFD T      R  +FFNW+YF + +  L + +
Sbjct: 159 AMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSSS 218

Query: 212 VVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNR 271
           ++V+IQ+N GWG GFGIPT+ M L++ +F  G+PLY   KP GSP+ R++QVVVA+F+  
Sbjct: 219 LLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRKS 278

Query: 272 KAVLPHDSNLL-----------------------WLDKAAVTTTPNLIT-DPPNLWRLAT 331
              +P D+ LL                       +LDKAAV +     + D  N WRL T
Sbjct: 279 SVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEEESKSGDYSNSWRLCT 338

Query: 332 VHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPATLSIFG 391
           V +VEELK +IRM PIWA+GI+     +   +  +QQ R MN  +  +FQ+PPA L  F 
Sbjct: 339 VTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKI-GSFQLPPAALGTFD 398

Query: 392 ILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFAINILATLVSSIIEIKRKKVA 451
             S++  + LYDR +VP A+K T    G T +QRMG+G  +++L    ++I+EI R  +A
Sbjct: 399 TASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMA 458

Query: 452 ANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWL 511
            + GL++   A +P SV W +PQ+++ G AEVF  +G LEF YDQSP+++RS  +AL  L
Sbjct: 459 NDLGLVES-GAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALL 518

Query: 512 AISVGNYVGTLMVYLVHKYT--GEEHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVYYVIC 553
             ++GNY+ +L++ LV  +T    +  W+ D NLN G L+Y++WL++G+ ++N+  Y   
Sbjct: 519 TNALGNYLSSLILTLVTYFTTRNGQEGWISD-NLNSGHLDYFFWLLAGLSLVNMAVYFFS 578

BLAST of HG10019859.1 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 407.5 bits (1046), Expect = 1.8e-113
Identity = 237/558 (42.47%), Postives = 337/558 (60.39%), Query Frame = 0

Query: 32  KKKLGGVKTMPFILGNEVCDRFASSGFHANIITYLTQDLNMPLVPASNILTNFSATSSFT 91
           +KK GG + + FILGNE  +R  S G  AN + YLT+  ++  V A+N++  +S  ++ T
Sbjct: 49  EKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLT 108

Query: 92  SLIGALIADSFAGRFWTITFASIIYELGMITITISAILPSLHPPPCPTQ--LNCTQASGT 151
            L+GA I+D++ GRF TI FAS    LG+ITIT++A  P LHP  C +Q  L+C   +  
Sbjct: 109 PLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKL 168

Query: 152 QLMFLYLALLLTSLGAGGIRPCVVAFAADQFDM-TKVGVAGRTWNFFNWYYFCMGMATLT 211
           Q+  L L L   S+G+GGIRPC + F  DQFD  T+ GV G   +FFNWYY    +  + 
Sbjct: 169 QIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKG-VASFFNWYYMTFTVVLII 228

Query: 212 ALTVVVYIQDNVGWGWGFGIPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAF 271
             TVVVYIQD V W  GF IPT  MAL+VV F  G   Y  +KP GS    +AQV+VAA 
Sbjct: 229 TQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAAR 288

Query: 272 KNRKAVLP----------------------HDSN-LLWLDKAAVTTTPNLITD--PPNLW 331
           K RK  LP                      H SN    LDKAAV    +L  +  P + W
Sbjct: 289 KKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEGDLTPEGPPADKW 348

Query: 332 RLATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPATL 391
           RL +V  VEE+K +IR++PIW+AGI+ + A + Q +FT+ QA  M+R+L P F+IP  +L
Sbjct: 349 RLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSL 408

Query: 392 SIFGILSMLTGLVLYDRLLVPFAKKLTNNPSGITCLQRMGVGFAINILATLVSSIIEIKR 451
           S+  +L++   L  YDR+ VPF +++T + SGIT LQR+G G    I + +V+ I+E  R
Sbjct: 409 SVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMR 468

Query: 452 KKVAANHGLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATA 511
           +  + N G   DPT   P SVFWL PQ  L G+ E F  +G +EF   Q PE +RS A +
Sbjct: 469 RIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANS 528

Query: 512 LYWLAISVGNYVGTLMVYLVHKYTG--EEHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVY 557
           L+ L+ +  +Y+ + +V +VHK++G  +  +WL ++NLN G+L+Y+Y+L++ + V+NLVY
Sbjct: 529 LFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWL-NKNLNAGKLDYFYYLIAVLGVVNLVY 588