HG10019442.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10019442.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	thioredoxin-like protein Clot
Location	Chr04: 21910409 .. 21910720 (-)
Sequence length	312
RNA-Seq Expression	HG10019442.1
Synteny	HG10019442.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAATCATCAATTCTCTCAAGAGATCTTAACTTCTGTTTGGTTTTTGTGTCAGACTGTGTGAGAGCTGAACCAGTCATCTACAAAAAACTGGAAGCAGCTTCAGATGATATCGCACTTTTGAGAGCCTATGTTGGAGATAGACCAACATGGAGGAATCCCCAGCATCCATGGAGAATGGATTCTAGGTTCAAGCTTACTGGAGTTCCAACGTTGGTTCGATGGGAGGAGAACGATAAAATCAGTGGTCGCCTCGAAGACCATGAAGCTCACGTGGACAACAAACTTGATGCCCTTATTGCTGGTAAATAA

mRNA sequence

ATGCAATCATCAATTCTCTCAAGAGATCTTAACTTCTGTTTGGTTTTTGTGTCAGACTGTGTGAGAGCTGAACCAGTCATCTACAAAAAACTGGAAGCAGCTTCAGATGATATCGCACTTTTGAGAGCCTATGTTGGAGATAGACCAACATGGAGGAATCCCCAGCATCCATGGAGAATGGATTCTAGGTTCAAGCTTACTGGAGTTCCAACGTTGGTTCGATGGGAGGAGAACGATAAAATCAGTGGTCGCCTCGAAGACCATGAAGCTCACGTGGACAACAAACTTGATGCCCTTATTGCTGGTAAATAA

Coding sequence (CDS)

ATGCAATCATCAATTCTCTCAAGAGATCTTAACTTCTGTTTGGTTTTTGTGTCAGACTGTGTGAGAGCTGAACCAGTCATCTACAAAAAACTGGAAGCAGCTTCAGATGATATCGCACTTTTGAGAGCCTATGTTGGAGATAGACCAACATGGAGGAATCCCCAGCATCCATGGAGAATGGATTCTAGGTTCAAGCTTACTGGAGTTCCAACGTTGGTTCGATGGGAGGAGAACGATAAAATCAGTGGTCGCCTCGAAGACCATGAAGCTCACGTGGACAACAAACTTGATGCCCTTATTGCTGGTAAATAA

Protein sequence

MQSSILSRDLNFCLVFVSDCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEENDKISGRLEDHEAHVDNKLDALIAGK

Homology

[Close]

BLAST of HG10019442.1 vs. NCBI nr
Match: XP_011651794.1 (thioredoxin-like protein Clot isoform X2 [Cucumis sativus])

HSP 1 Score: 178.3 bits (451), Expect = 3.3e-41
Identity = 81/85 (95.29%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 19  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEEN 78
           DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWR+D RFKLTGVPTLVRWEEN
Sbjct: 49  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRVDPRFKLTGVPTLVRWEEN 108

Query: 79  DKISGRLEDHEAHVDNKLDALIAGK 104
           DKISGRLEDHEAHV+NK+DALIAGK
Sbjct: 109 DKISGRLEDHEAHVENKIDALIAGK 133

[Close]

BLAST of HG10019442.1 vs. NCBI nr
Match: KAE8650938.1 (hypothetical protein Csa_001510 [Cucumis sativus])

HSP 1 Score: 177.6 bits (449), Expect = 5.7e-41
Identity = 81/90 (90.00%), Postives = 86/90 (95.56%), Query Frame = 0


Query: 14  LVFVSDCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLV 73
           +  +S CVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWR+D RFKLTGVPTLV
Sbjct: 46  ICLISYCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRVDPRFKLTGVPTLV 105

Query: 74  RWEENDKISGRLEDHEAHVDNKLDALIAGK 104
           RWEENDKISGRLEDHEAHV+NK+DALIAGK
Sbjct: 106 RWEENDKISGRLEDHEAHVENKIDALIAGK 135

[Close]

BLAST of HG10019442.1 vs. NCBI nr
Match: XP_016902770.1 (PREDICTED: thioredoxin-like protein Clot isoform X2 [Cucumis melo])

HSP 1 Score: 176.4 bits (446), Expect = 1.3e-40
Identity = 80/85 (94.12%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 19  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEEN 78
           DCVRAEPVIYKKLEAASDD ALLRAYVGDRPTWRNPQHPWR+D RFKLTGVPTLVRWEEN
Sbjct: 49  DCVRAEPVIYKKLEAASDDTALLRAYVGDRPTWRNPQHPWRVDCRFKLTGVPTLVRWEEN 108

Query: 79  DKISGRLEDHEAHVDNKLDALIAGK 104
           DKISGRLEDHEAHV+NK+DALIAGK
Sbjct: 109 DKISGRLEDHEAHVENKIDALIAGK 133

[Close]

BLAST of HG10019442.1 vs. NCBI nr
Match: KAA0048313.1 (nuclear envelope integral membrane protein 1 isoform X1 [Cucumis melo var. makuwa] >TYK10658.1 nuclear envelope integral membrane protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 174.1 bits (440), Expect = 6.3e-40
Identity = 79/84 (94.05%), Postives = 82/84 (97.62%), Query Frame = 0


Query: 20  CVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEEND 79
           CVRAEPVIYKKLEAASDD ALLRAYVGDRPTWRNPQHPWR+D RFKLTGVPTLVRWEEND
Sbjct: 489 CVRAEPVIYKKLEAASDDTALLRAYVGDRPTWRNPQHPWRVDCRFKLTGVPTLVRWEEND 548

Query: 80  KISGRLEDHEAHVDNKLDALIAGK 104
           KISGRLEDHEAHV+NK+DALIAGK
Sbjct: 549 KISGRLEDHEAHVENKIDALIAGK 572

[Close]

BLAST of HG10019442.1 vs. NCBI nr
Match: KAG6581598.1 (O-fucosyltransferase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 171.4 bits (433), Expect = 4.1e-39
Identity = 79/97 (81.44%), Postives = 87/97 (89.69%), Query Frame = 0


Query: 5   ILSRDLNFCLVFVSDCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRF 64
           +  +D +  L +  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNP+HPWR+DSRF
Sbjct: 630 LADKDPSTSLSWCPDCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPRHPWRVDSRF 689

Query: 65  KLTGVPTLVRWEENDKISGRLEDHEAHVDNKLDALIA 102
           KLTGVPTLVRWE NDKISGRLEDHEAHV+ K+DALIA
Sbjct: 690 KLTGVPTLVRWEANDKISGRLEDHEAHVEKKIDALIA 726

[Close]

BLAST of HG10019442.1 vs. ExPASy Swiss-Prot
Match: Q9FMN4 (Thioredoxin-like protein Clot OS=Arabidopsis thaliana OX=3702 GN=At5g42850 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 1.5e-28
Identity = 55/83 (66.27%), Postives = 67/83 (80.72%), Query Frame = 0


Query: 19  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEEN 78
           DCVRAEPVIYK LE   +++ L+RAY GDRPTWR P HPWR+DSRFKLTGVPTLVRW + 
Sbjct: 50  DCVRAEPVIYKTLEEFPEEVKLIRAYAGDRPTWRTPAHPWRVDSRFKLTGVPTLVRW-DG 109

Query: 79  DKISGRLEDHEAHVDNKLDALIA 102
           D + GRLEDH+AH+ + +  L+A
Sbjct: 110 DSVKGRLEDHQAHLPHLILPLLA 131

[Close]

BLAST of HG10019442.1 vs. ExPASy Swiss-Prot
Match: Q5Z9Z3 (Thioredoxin-like protein Clot OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0320000 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 5.9e-25
Identity = 54/100 (54.00%), Postives = 71/100 (71.00%), Query Frame = 0


Query: 5   ILSRDLNFCLVFVSDCVRAEPVIYKKLEAAS----DDIALLRAYVGDRPTWRNPQHPWRM 64
           +  RD +    +  DC  AEPVIY ++EAA+     D+ LLRAYVGD+PTWR+P HPWR 
Sbjct: 37  LADRDASSNQTWCPDCNVAEPVIYDRVEAAAKGKEKDVVLLRAYVGDKPTWRDPAHPWRA 96

Query: 65  DSRFKLTGVPTLVRWEENDKISGRLEDHEAHVDNKLDALI 101
           D RF+LTGVPTL+RW EN   + RL D EAH+ +K+DA++
Sbjct: 97  DPRFRLTGVPTLIRW-ENGAAAARLGDDEAHLADKVDAVV 135

[Close]

BLAST of HG10019442.1 vs. ExPASy Swiss-Prot
Match: Q6DBT3 (Thioredoxin domain-containing protein 17 OS=Danio rerio OX=7955 GN=txndc17 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.8e-06
Identity = 24/63 (38.10%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 19  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEEN 78
           DCV+AEPV+  +L    +    +   VGDRP W++  + ++     KLTGVPTL+R+   
Sbjct: 45  DCVKAEPVVRAELPHLPEGTVFIYCQVGDRPYWKDSNNDFK--KTLKLTGVPTLLRYGTP 104

Query: 79  DKI 82
            K+
Sbjct: 105 QKL 105

[Close]

BLAST of HG10019442.1 vs. ExPASy TrEMBL
Match: A0A0A0LEX6 (DUF953 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G701560 PE=4 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.6e-41
Identity = 81/85 (95.29%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 19  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEEN 78
           DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWR+D RFKLTGVPTLVRWEEN
Sbjct: 49  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRVDPRFKLTGVPTLVRWEEN 108

Query: 79  DKISGRLEDHEAHVDNKLDALIAGK 104
           DKISGRLEDHEAHV+NK+DALIAGK
Sbjct: 109 DKISGRLEDHEAHVENKIDALIAGK 133

[Close]

BLAST of HG10019442.1 vs. ExPASy TrEMBL
Match: A0A1S4E462 (thioredoxin-like protein Clot isoform X2 OS=Cucumis melo OX=3656 GN=LOC103500299 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 6.1e-41
Identity = 80/85 (94.12%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 19  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEEN 78
           DCVRAEPVIYKKLEAASDD ALLRAYVGDRPTWRNPQHPWR+D RFKLTGVPTLVRWEEN
Sbjct: 49  DCVRAEPVIYKKLEAASDDTALLRAYVGDRPTWRNPQHPWRVDCRFKLTGVPTLVRWEEN 108

Query: 79  DKISGRLEDHEAHVDNKLDALIAGK 104
           DKISGRLEDHEAHV+NK+DALIAGK
Sbjct: 109 DKISGRLEDHEAHVENKIDALIAGK 133

[Close]

BLAST of HG10019442.1 vs. ExPASy TrEMBL
Match: A0A5D3CHE8 (Nuclear envelope integral membrane protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00200 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.0e-40
Identity = 79/84 (94.05%), Postives = 82/84 (97.62%), Query Frame = 0


Query: 20  CVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEEND 79
           CVRAEPVIYKKLEAASDD ALLRAYVGDRPTWRNPQHPWR+D RFKLTGVPTLVRWEEND
Sbjct: 489 CVRAEPVIYKKLEAASDDTALLRAYVGDRPTWRNPQHPWRVDCRFKLTGVPTLVRWEEND 548

Query: 80  KISGRLEDHEAHVDNKLDALIAGK 104
           KISGRLEDHEAHV+NK+DALIAGK
Sbjct: 549 KISGRLEDHEAHVENKIDALIAGK 572

[Close]

BLAST of HG10019442.1 vs. ExPASy TrEMBL
Match: A0A6J1J7Z3 (thioredoxin-like protein Clot OS=Cucurbita maxima OX=3661 GN=LOC111482184 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.0e-39
Identity = 79/97 (81.44%), Postives = 87/97 (89.69%), Query Frame = 0


Query: 5   ILSRDLNFCLVFVSDCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRF 64
           +  +D +  L +  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNP+HPWR+DSRF
Sbjct: 35  LADKDPSTSLSWCPDCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPRHPWRVDSRF 94

Query: 65  KLTGVPTLVRWEENDKISGRLEDHEAHVDNKLDALIA 102
           KLTGVPTLVRWE NDKISGRLEDHEAHV+ K+DALIA
Sbjct: 95  KLTGVPTLVRWEANDKISGRLEDHEAHVEKKIDALIA 131

[Close]

BLAST of HG10019442.1 vs. ExPASy TrEMBL
Match: A0A6J1F6J0 (thioredoxin-like protein Clot OS=Cucurbita moschata OX=3662 GN=LOC111441330 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.0e-39
Identity = 79/97 (81.44%), Postives = 87/97 (89.69%), Query Frame = 0


Query: 5   ILSRDLNFCLVFVSDCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRF 64
           +  +D +  L +  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNP+HPWR+DSRF
Sbjct: 35  LADKDPSTSLSWCPDCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPRHPWRVDSRF 94

Query: 65  KLTGVPTLVRWEENDKISGRLEDHEAHVDNKLDALIA 102
           KLTGVPTLVRWE NDKISGRLEDHEAHV+ K+DALIA
Sbjct: 95  KLTGVPTLVRWEANDKISGRLEDHEAHVEKKIDALIA 131

[Close]

BLAST of HG10019442.1 vs. TAIR 10
Match: AT5G42850.1 (Thioredoxin superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 1.1e-29
Identity = 55/83 (66.27%), Postives = 67/83 (80.72%), Query Frame = 0


Query: 19  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEEN 78
           DCVRAEPVIYK LE   +++ L+RAY GDRPTWR P HPWR+DSRFKLTGVPTLVRW + 
Sbjct: 50  DCVRAEPVIYKTLEEFPEEVKLIRAYAGDRPTWRTPAHPWRVDSRFKLTGVPTLVRW-DG 109

Query: 79  DKISGRLEDHEAHVDNKLDALIA 102
           D + GRLEDH+AH+ + +  L+A
Sbjct: 110 DSVKGRLEDHQAHLPHLILPLLA 131

[Close]

BLAST of HG10019442.1 vs. TAIR 10
Match: AT5G42850.2 (Thioredoxin superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 1.1e-29
Identity = 55/83 (66.27%), Postives = 67/83 (80.72%), Query Frame = 0


Query: 19  DCVRAEPVIYKKLEAASDDIALLRAYVGDRPTWRNPQHPWRMDSRFKLTGVPTLVRWEEN 78
           DCVRAEPVIYK LE   +++ L+RAY GDRPTWR P HPWR+DSRFKLTGVPTLVRW + 
Sbjct: 50  DCVRAEPVIYKTLEEFPEEVKLIRAYAGDRPTWRTPAHPWRVDSRFKLTGVPTLVRW-DG 109

Query: 79  DKISGRLEDHEAHVDNKLDALIA 102
           D + GRLEDH+AH+ + +  L+A
Sbjct: 110 DSVKGRLEDHQAHLPHLILPLLA 131

The following BLAST results are available for this feature:

BLAST of HG10019442.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651794.1	3.3e-41	95.29	thioredoxin-like protein Clot isoform X2 [Cucumis sativus]	[more]
KAE8650938.1	5.7e-41	90.00	hypothetical protein Csa_001510 [Cucumis sativus]	[more]
XP_016902770.1	1.3e-40	94.12	PREDICTED: thioredoxin-like protein Clot isoform X2 [Cucumis melo]	[more]
KAA0048313.1	6.3e-40	94.05	nuclear envelope integral membrane protein 1 isoform X1 [Cucumis melo var. makuw...	[more]
KAG6581598.1	4.1e-39	81.44	O-fucosyltransferase 20, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of HG10019442.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FMN4	1.5e-28	66.27	Thioredoxin-like protein Clot OS=Arabidopsis thaliana OX=3702 GN=At5g42850 PE=2 ...	[more]
Q5Z9Z3	5.9e-25	54.00	Thioredoxin-like protein Clot OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0...	[more]
Q6DBT3	2.8e-06	38.10	Thioredoxin domain-containing protein 17 OS=Danio rerio OX=7955 GN=txndc17 PE=2 ...	[more]

BLAST of HG10019442.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LEX6	1.6e-41	95.29	DUF953 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G701560 PE=4...	[more]
A0A1S4E462	6.1e-41	94.12	thioredoxin-like protein Clot isoform X2 OS=Cucumis melo OX=3656 GN=LOC103500299...	[more]
A0A5D3CHE8	3.0e-40	94.05	Nuclear envelope integral membrane protein 1 isoform X1 OS=Cucumis melo var. mak...	[more]
A0A6J1J7Z3	2.0e-39	81.44	thioredoxin-like protein Clot OS=Cucurbita maxima OX=3661 GN=LOC111482184 PE=4 S...	[more]
A0A6J1F6J0	2.0e-39	81.44	thioredoxin-like protein Clot OS=Cucurbita moschata OX=3662 GN=LOC111441330 PE=4...	[more]

BLAST of HG10019442.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42850.1	1.1e-29	66.27	Thioredoxin superfamily protein	[more]
AT5G42850.2	1.1e-29	66.27	Thioredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010357	Thioredoxin domain-containing protein 17-like domain	PFAM	PF06110	DUF953	coord: 18..98 e-value: 2.9E-26 score: 91.4
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 8..102 e-value: 8.5E-22 score: 79.4
None	No IPR available	PANTHER	PTHR12452:SF0	THIOREDOXIN DOMAIN-CONTAINING PROTEIN 17	coord: 16..101
IPR045108	Thioredoxin domain-containing protein 17-like	PANTHER	PTHR12452	42-9-9 PROTEIN-RELATED	coord: 16..101
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 19..87

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10019442	HG10019442	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10019442.1-cds	HG10019442.1-cds-Chr04:21910409..21910720	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10019442.1	HG10019442.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098869	cellular oxidant detoxification
cellular_component	GO:0005829	cytosol
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005637	nuclear inner membrane
molecular_function	GO:0004601	peroxidase activity
molecular_function	GO:0047134	protein-disulfide reductase (NAD(P)) activity