HG10019409.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10019409.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Methyltransferase
Location	Chr04: 21496580 .. 21499470 (-)
Sequence length	786
RNA-Seq Expression	HG10019409.1
Synteny	HG10019409.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAAAGCTGGAGCGTTGGATCTTGCATCGGGCCTTGGCGGCAAGTTGGAAAAGAGCGAGGTCCTTTCCGCCGTTGAAAAGTAAAACATTCCCTCTCTCTTTCTACCCCCCAGATTGGTTTTTGCTTGGTTAATGGACTTATAACTTTAAAATCATGTATCTGCTTTTGTATGTGGTTGTGTTTTGGGCTGTTCAGAGAAACTAAACGAGCTAGCTCATTCTCTTTTAGAGACATCTAGTTTGAATTCTACGTTTAAGCTTCTGCATGTTCTGTTTTTTTCTTGTTGTGCAATTGGGTTATGACCTCTTGATCTGTTTTTGTTAATTTTAGATGTGGATGGTTTGAATGAAAACAGGGCATTCTAGGATTGTGTACAAATTACAATGTTGTGCGCAGTGACATGTAGTAGGAGTGGAAATATTGTGTAGAATTTCTTGAATTGGATTAAGCTTCAGCCTGATTTGTTGTATTTGAATTTTGGTTTGTTGTATCTTTGTTATTATGAGCTCATTCTGTTCTGAAAGTGTTGCATTGTTGGATCAGGTATGAGAAGTACCATGTCTGTTATGGAGGTGAAGAGGAGGAGAGAAAAGCTAACTACACTGACATGGTAAAAGAAGATTGCTGTTCTCATTGTCTTTCTTTTTGATGTTCTCATAAAGTAGAATCACCCTCTTTGTCATTTTTATATGGTCGTGTATTATGTATTACTACAGGTTAACAAATACTATGATCTTGTTACAAGCTTTTACGAGTTCGGTTGGGGTGAGTCATTCCATTTTGCACCTAGGTATGACGTACTATCTTCCTCCTTCTGCACTCATCAGACCAGAATGTGTCTCTGTTTTTTGCAATCTCGAATCTAAACATGCATATTTTCCAATCTAGATGGAAAGGTGAATCTCTGCGGGAGAGTATTAAGAGGCACGAGCACTTCCTTGCTTTGCAATTAGGTTTGAAACCTGGATATAAGGTTTGTGTTTTTGAGTTTTTCTATACAATGATGGAGAAATCTTCTTCATCCATCTAGATGGAAAGGTGAATCTCTGCGGGAGTTATTAAGAGGCACGAGCACTTCCTTGCTTTGCAATTAGGTTTGAAACCTGGATATAAGGTTTGTGTTTTTGAGTTTTGCTATACAATGATGGAGAAATCTTCTTCATCCGTTTCAAATTATGGAGAATAACTTTGCCTATATTTTTAGGTGTTGGATGTTTGATGTGGAATGGTGACCCACTTAGAGAAATTGCGAGATTTAGGTGTGATTCACTAGCTAACTTTGAGTCTTCTTTTTTCCTTTGATGATTGATGCTAAAATAAATATCACGCTACCTACCCTGAATTGGAATTATAATAATTCTAACAACTGCATTGTTAACGGTCTCAATTCAACACTTTGATGCTGGATCGAGTATATATAGTTTTATACCTTTTGTTTTATCTTTATGTTTATGGTTTGTATCTCATATCATATGACACTCTGTTGCAGTTCTACTTCAGTTACTGGATTAAACACCAATGATTACCAGATTTCAAGAGGAAAGGTAGTATTTTTTTTTCCTAATCCTTTGATGAAGAATGTCAGGAACATGTTTTCTTAGAAATATCTTACTACTGGTCGTAAGATTTTGTTGATTTTCCTTTCTTACCTTCTTCATCGAGTCATTTGACATAATTATTATTCTGCTATTATTATGTTCTGATCAAGACTTGCAGTAAGATAACTGTAATTTTTGCTTTTACTTCGCCAGGAATTGAATCGCGTTGCAGAGTGGGCCAAAACTTGTGACTTTGTCAAGGTTGGTTTTTTGTAGATTACGTGTAGTCTTTCAAAACTTTGTTTGTTTACTATCTTAATTTGTTTGTATTTCTTTTAGAAGATGAAAGAAAGACGAACGGTTATGTTTCAAAGGTTAATACTTTATTCATTTTCTCCATGCTGCAGGCCGACTTCATGAAAATGCCGTTTCCTGACATTCATTGATTCAATATATGCAATTGAAGCTACTTGTCATGCACCAGACGCAGTAAGCCTTAAAATTACATTCTGCAGTGTTAATTTCAGTTTACAGTTTACAGCAATAATTGTTGATCTAGCATGAGTATCATCTGCGAGATTATATGTGTGTTAGCCATCTTGGTTTGACTTTTTTTTCCTGTCTTGCAGTATGGGTGCTATAAGGAGATTTATAGAGTACTAAAGCCTGGCCAACATTTTGCTGCTTATGAATGGTGTATGACTGATGCTTTTGATCCAAATAATCAAGAACATCAAAAGATAAAGGTACCCGGCTTCATCCCTTCTATTTAGCAAATTCTTTGATTGTACTACACTGATTGATCCTATACGAATTACATTGACCAAGTCCCAATCGTGCAGGCAGAAATTGAGATCGGTGATGGTCTTCCAGATATCAGGTTGACAGGAAAATGCCTTGAAGCTTTAAAACAAGCAGGCTTTGAGGTTAGTTGGAAAATAACCTCACATTATTAGTTTCAATTATTGTTGTAGCTTTTATCCTTGAACTATCATTAAAAGCATTGATCTCTCATATCCATGTGCACATGACATCAGGTTGTTTGGGAGAAAGATCTTGCTGCAAATTCGCCTCTTCCTTGGTACTTGCCTTTAGACAAAAGCCATTTCTCACTGAGTAGCTTCCGTCTAACAGCCCTTGGTCGTTTCATTACTAAAAATATGGTAAGCTTTATCTGATGTGACCTTGACGAGTCGTCTTTTCTCCACAATGAAACGTGAAGGAGAGAACCTTATTTCTTTTTGTAGGTCAAAGCACTGGAGTTCATTCGACTCGCCCCCAAGGGTAGCCAAAGAGTTCAAGACTTTCTAGAGAAAGCTGCTGAAGGGCTAGTTGAGGGTGGAAAGTGA

mRNA sequence

ATGTCGAAAGCTGGAGCGTTGGATCTTGCATCGGGCCTTGGCGGCAAGTTGGAAAAGAGCGAGGTCCTTTCCGCCGTTGAAAAGTATGAGAAGTACCATGTCTGTTATGGAGGTGAAGAGGAGGAGAGAAAAGCTAACTACACTGACATGGTTAACAAATACTATGATCTTGTTACAAGCTTTTACGAGTTCGGTTGGGGTGAGTCATTCCATTTTGCACCTAGATGGAAAGGTGAATCTCTGCGGGAGAGTATTAAGAGGCACGAGCACTTCCTTGCTTTGCAATTAGGTTTGAAACCTGGATATAAGGAATTGAATCGCGTTGCAGAGTGGGCCAAAACTTGTGACTTTGTCAAGTATGGGTGCTATAAGGAGATTTATAGAGTACTAAAGCCTGGCCAACATTTTGCTGCTTATGAATGGTGTATGACTGATGCTTTTGATCCAAATAATCAAGAACATCAAAAGATAAAGGCAGAAATTGAGATCGGTGATGGTCTTCCAGATATCAGGTTGACAGGAAAATGCCTTGAAGCTTTAAAACAAGCAGGCTTTGAGGTTGTTTGGGAGAAAGATCTTGCTGCAAATTCGCCTCTTCCTTGGTACTTGCCTTTAGACAAAAGCCATTTCTCACTGAGTAGCTTCCGTCTAACAGCCCTTGGTCGTTTCATTACTAAAAATATGGTCAAAGCACTGGAGTTCATTCGACTCGCCCCCAAGGGTAGCCAAAGAGTTCAAGACTTTCTAGAGAAAGCTGCTGAAGGGCTAGTTGAGGGTGGAAAGTGA

Coding sequence (CDS)

ATGTCGAAAGCTGGAGCGTTGGATCTTGCATCGGGCCTTGGCGGCAAGTTGGAAAAGAGCGAGGTCCTTTCCGCCGTTGAAAAGTATGAGAAGTACCATGTCTGTTATGGAGGTGAAGAGGAGGAGAGAAAAGCTAACTACACTGACATGGTTAACAAATACTATGATCTTGTTACAAGCTTTTACGAGTTCGGTTGGGGTGAGTCATTCCATTTTGCACCTAGATGGAAAGGTGAATCTCTGCGGGAGAGTATTAAGAGGCACGAGCACTTCCTTGCTTTGCAATTAGGTTTGAAACCTGGATATAAGGAATTGAATCGCGTTGCAGAGTGGGCCAAAACTTGTGACTTTGTCAAGTATGGGTGCTATAAGGAGATTTATAGAGTACTAAAGCCTGGCCAACATTTTGCTGCTTATGAATGGTGTATGACTGATGCTTTTGATCCAAATAATCAAGAACATCAAAAGATAAAGGCAGAAATTGAGATCGGTGATGGTCTTCCAGATATCAGGTTGACAGGAAAATGCCTTGAAGCTTTAAAACAAGCAGGCTTTGAGGTTGTTTGGGAGAAAGATCTTGCTGCAAATTCGCCTCTTCCTTGGTACTTGCCTTTAGACAAAAGCCATTTCTCACTGAGTAGCTTCCGTCTAACAGCCCTTGGTCGTTTCATTACTAAAAATATGGTCAAAGCACTGGAGTTCATTCGACTCGCCCCCAAGGGTAGCCAAAGAGTTCAAGACTTTCTAGAGAAAGCTGCTGAAGGGCTAGTTGAGGGTGGAAAGTGA

Protein sequence

MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGYKELNRVAEWAKTCDFVKYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGKCLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFIRLAPKGSQRVQDFLEKAAEGLVEGGK

Homology

[Close]

BLAST of HG10019409.1 vs. NCBI nr
Match: XP_038903118.1 (cycloartenol-C-24-methyltransferase isoform X1 [Benincasa hispida] >XP_038903119.1 cycloartenol-C-24-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 492.3 bits (1266), Expect = 2.6e-135
Identity = 249/326 (76.38%), Postives = 257/326 (78.83%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MSKAGALDLASGLGGKL+K+EVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS
Sbjct: 1   MSKAGALDLASGLGGKLDKNEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY                  
Sbjct: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGYKVLDVGCGIGGPLREIAR 120

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA+  KTCDF+K                        
Sbjct: 121 FSSTSVTGLNNNEYQISRGKELNRVAKVDKTCDFIKADFMKMPFPDNSFDAVYAIEATCH 180

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
               YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK
Sbjct: 181 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           CLEALK+AGFE++WEKDLA NSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVK LEF+
Sbjct: 241 CLEALKEAGFEILWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKVLEFV 300

[Close]

BLAST of HG10019409.1 vs. NCBI nr
Match: XP_008461816.1 (PREDICTED: cycloartenol-C-24-methyltransferase [Cucumis melo] >XP_008461817.1 PREDICTED: cycloartenol-C-24-methyltransferase [Cucumis melo])

HSP 1 Score: 491.5 bits (1264), Expect = 4.5e-135
Identity = 252/326 (77.30%), Postives = 255/326 (78.22%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MSK GALDLASGLGGKLEK++VLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS
Sbjct: 1   MSKTGALDLASGLGGKLEKNDVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQL LKPGY                  
Sbjct: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLDLKPGYKVLDVGCGIGGPLREIAR 120

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA+  KTCDFVK                        
Sbjct: 121 FSYTSVTGLNNNEYQISRGKELNRVAKVDKTCDFVKADFMKMPFPDNSFDAVYAIEATCH 180

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
               YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK
Sbjct: 181 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           CLEALKQAGFEVVWEKDLA NSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVK LEFI
Sbjct: 241 CLEALKQAGFEVVWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKVLEFI 300

[Close]

BLAST of HG10019409.1 vs. NCBI nr
Match: XP_004149721.1 (cycloartenol-C-24-methyltransferase [Cucumis sativus])

HSP 1 Score: 491.5 bits (1264), Expect = 4.5e-135
Identity = 251/326 (76.99%), Postives = 256/326 (78.53%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MSK GALDLASGLGGKLEK+EVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS
Sbjct: 1   MSKTGALDLASGLGGKLEKNEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQL LKPGY                  
Sbjct: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLDLKPGYKVLDVGCGIGGPLREIAR 120

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA+  +TCDFVK                        
Sbjct: 121 FSYTSVTGLNNNEYQISRGKELNRVAKVDRTCDFVKADFMKMPFPDNSFDAVYAIEATCH 180

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
               YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIR+TGK
Sbjct: 181 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRMTGK 240

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           CLEALKQAGFEV+WEKDLA NSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI
Sbjct: 241 CLEALKQAGFEVIWEKDLAENSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 300

[Close]

BLAST of HG10019409.1 vs. NCBI nr
Match: KAE8650927.1 (hypothetical protein Csa_000930 [Cucumis sativus])

HSP 1 Score: 491.5 bits (1264), Expect = 4.5e-135
Identity = 251/326 (76.99%), Postives = 256/326 (78.53%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MSK GALDLASGLGGKLEK+EVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS
Sbjct: 77  MSKTGALDLASGLGGKLEKNEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 136

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQL LKPGY                  
Sbjct: 137 FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLDLKPGYKVLDVGCGIGGPLREIAR 196

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA+  +TCDFVK                        
Sbjct: 197 FSYTSVTGLNNNEYQISRGKELNRVAKVDRTCDFVKADFMKMPFPDNSFDAVYAIEATCH 256

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
               YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIR+TGK
Sbjct: 257 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRMTGK 316

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           CLEALKQAGFEV+WEKDLA NSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI
Sbjct: 317 CLEALKQAGFEVIWEKDLAENSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 376

[Close]

BLAST of HG10019409.1 vs. NCBI nr
Match: KAA0048385.1 (cycloartenol-C-24-methyltransferase [Cucumis melo var. makuwa] >TYJ99862.1 cycloartenol-C-24-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 491.5 bits (1264), Expect = 4.5e-135
Identity = 252/326 (77.30%), Postives = 255/326 (78.22%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MSK GALDLASGLGGKLEK++VLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS
Sbjct: 1   MSKTGALDLASGLGGKLEKNDVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQL LKPGY                  
Sbjct: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLDLKPGYKVLDVGCGIGGPLREIAR 120

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA+  KTCDFVK                        
Sbjct: 121 FSYTSVTGLNNNEYQISRGKELNRVAKVDKTCDFVKADFMKMPFPDNSFDAVYAIEATCH 180

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
               YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK
Sbjct: 181 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           CLEALKQAGFEVVWEKDLA NSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVK LEFI
Sbjct: 241 CLEALKQAGFEVVWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKVLEFI 300

[Close]

BLAST of HG10019409.1 vs. ExPASy Swiss-Prot
Match: Q9LM02 (Cycloartenol-C-24-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=SMT1 PE=1 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 4.0e-118
Identity = 215/320 (67.19%), Postives = 239/320 (74.69%), Query Frame = 0


Query: 7   LDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGW 66
           +DLAS LGGK++KS+VL+AVEKYE+YHV +GG EEERKANYTDMVNKYYDL TSFYE+GW
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGW 60

Query: 67  GESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------------ 126
           GESFHFA RWKGESLRESIKRHEHFLALQLG++PG                         
Sbjct: 61  GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVV 120

Query: 127 -------------KELNRVAEWAKTCDFVK----------------------------YG 186
                        KELNR+A   KTC+FVK                            YG
Sbjct: 121 TGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYG 180

Query: 187 CYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGKCLEALK 246
           CYKEIYRVLKPGQ FAAYEWCMTDAFDP+N EHQKIK EIEIGDGLPDIRLT KCLEALK
Sbjct: 181 CYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALK 240

Query: 247 QAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFIRLAPKG 262
           QAGFEV+WEKDLA +SP+PWYLPLDK+HFSLSSFRLTA+GRFITKNMVK LE+IRLAP+G
Sbjct: 241 QAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQG 300

[Close]

BLAST of HG10019409.1 vs. ExPASy Swiss-Prot
Match: Q6ZIX2 (Cycloartenol-C-24-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Smt1-1 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 2.4e-107
Identity = 198/326 (60.74%), Postives = 229/326 (70.25%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MS++GA+DLASGLGGK+ K EV SAV++YEKYH  YGG+EE RK+NYTDMVNKYYDL TS
Sbjct: 1   MSRSGAMDLASGLGGKITKDEVKSAVDEYEKYHGYYGGKEEARKSNYTDMVNKYYDLATS 60

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYE+GWGESFHFA RW GESLRESIKRHEHFLALQLG+KPG                   
Sbjct: 61  FYEYGWGESFHFAHRWNGESLRESIKRHEHFLALQLGVKPGMKVLDVGCGIGGPLREIAK 120

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA  + TCDFVK                        
Sbjct: 121 FSLASVTGLNNNEYQITRGKELNRVAGVSGTCDFVKADFMKMPFSDNTFDAVYAIEATCH 180

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
                GCYKEIYRVLKPGQ FA YEWC+TD ++PNN  H++IK EIE+G+GLPDIR T +
Sbjct: 181 APDPVGCYKEIYRVLKPGQCFAVYEWCITDHYEPNNATHKRIKDEIELGNGLPDIRSTQQ 240

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           CL+A K AGFEV+W+KDLA +SP+PWYLPLD S FSLSSFRLT +GR IT+ MVKALE++
Sbjct: 241 CLQAAKDAGFEVIWDKDLAEDSPVPWYLPLDPSRFSLSSFRLTTVGRAITRTMVKALEYV 300

[Close]

BLAST of HG10019409.1 vs. ExPASy Swiss-Prot
Match: Q54I98 (Probable cycloartenol-C-24-methyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=smt1 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.5e-48
Identity = 110/286 (38.46%), Postives = 144/286 (50.35%), Query Frame = 0


Query: 41  EERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKP 100
           + RK NYT MVN +YDL T FYEFGWG+SFHFA R K ES   SI RHE ++A QLGL P
Sbjct: 52  QARKNNYTHMVNTFYDLATDFYEFGWGQSFHFATRHKYESFEASIARHEMYMAHQLGLFP 111

Query: 101 GY-------------------------------------KELNRVAEWAKTCDFVK---- 160
           G                                      K LN  A  +  C F+K    
Sbjct: 112 GMKVIDIGCGVGGPMRTIARFSGANVVGLNNNEYQIQRGKRLNESAGLSHLCSFIKADFM 171

Query: 161 ------------------------YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQ 220
                                    G YKE++R++KPG  F  YEW MT+ F+P +    
Sbjct: 172 HVPVEDNTYDCAYQIEATCHAPDLVGLYKEVFRIVKPGGLFGGYEWIMTNKFNPEDPVEV 231

Query: 221 KIKAEIEIGDGLPDIRLTGKCLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSF 262
            IK +IE+G+GLPD+    + + A K AGFEV+   D+A  S LPWYLPL  S  S++ F
Sbjct: 232 NIKKQIELGNGLPDLVKPAEIINAAKAAGFEVITAFDVAETSELPWYLPL-SSGVSITGF 291

[Close]

BLAST of HG10019409.1 vs. ExPASy Swiss-Prot
Match: O74198 (Sterol 24-C-methyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ERG6 PE=3 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.0e-44
Identity = 116/328 (35.37%), Postives = 160/328 (48.78%), Query Frame = 0


Query: 11  SGLGGKLEKSEVLSAVEKYEKYHVCYGG----EEEERKANYTDMVNKYYDLVTSFYEFGW 70
           +GL   + KS+  ++V     +    GG    +EE+R  +Y+ + + YY+LVT FYE+GW
Sbjct: 29  TGLSALIAKSKDAASVAAEGYFKHWDGGISKDDEEKRLNDYSQLTHHYYNLVTDFYEYGW 88

Query: 71  GESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGYK----------------------- 130
           G SFHF+  +KGE+ R++  RHEHFLA ++ L    K                       
Sbjct: 89  GSSFHFSRYYKGEAFRQATARHEHFLAHKMNLNENMKVLDVGCGVGGPGREITRFTDCEI 148

Query: 131 --------ELNRVAEWAK----------------TCDF------------------VKYG 190
                   ++ R   +AK                  DF                  V  G
Sbjct: 149 VGLNNNDYQIERANHYAKKYHLDHKLSYVKGDFMQMDFEPESFDAVYAIEATVHAPVLEG 208

Query: 191 CYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGKCLEALK 250
            Y EIY+VLKPG  F  YEW MTD +D  N+EH+KI   IE+GDG+P +       +ALK
Sbjct: 209 VYSEIYKVLKPGGVFGVYEWVMTDKYDETNEEHRKIAYGIEVGDGIPKMYSRKVAEQALK 268

Query: 251 QAGFEVVWEKDLA-ANSPLPWYLPLDKS-------HFSLSSFRLTALGRFITKNMVKALE 262
             GFE+ ++KDLA  +  +PWY PL             L+ FR + +GRFIT   V  +E
Sbjct: 269 NVGFEIEYQKDLADVDDEIPWYYPLSGDLKFCQTFGDYLTVFRTSRIGRFITTESVGLME 328

[Close]

BLAST of HG10019409.1 vs. ExPASy Swiss-Prot
Match: Q875K1 (Sterol 24-C-methyltransferase OS=Clavispora lusitaniae (strain ATCC 42720) OX=306902 GN=ERG6 PE=3 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.9e-43
Identity = 110/296 (37.16%), Postives = 149/296 (50.34%), Query Frame = 0


Query: 39  EEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQL-- 98
           +E++R  +Y+ +   YY+LVT FYE+GWG SFHF+  +KGE+ R++  RHEHFLA ++  
Sbjct: 61  DEKKRLDDYSSLTKNYYNLVTDFYEYGWGSSFHFSRYYKGEAFRQATARHEHFLAHKMNI 120

Query: 99  -----------------------------GLKPGYKELNRVAEWAK-------------- 158
                                        GL     ++ R   +AK              
Sbjct: 121 NENMRVLDVGCGVGGPGREICRFTDCTIVGLNNNDYQVERAQYYAKKYKLDDKLSYVKGD 180

Query: 159 --TCDF------------------VKYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQE 218
               DF                  V  G Y EIY+VLKPG  F  YEW MTD +D NN+E
Sbjct: 181 FMQMDFEPESFDAVYAIEATVHAPVLEGVYSEIYKVLKPGGVFGVYEWVMTDKYDENNEE 240

Query: 219 HQKIKAEIEIGDGLPDIRLTGKCLEALKQAGFEVVWEKDLA-ANSPLPWYLPLD---KSH 262
           H+KI   IE+GDG+P +       +ALK  GF++ +EKDLA  N  +PWY PL    K  
Sbjct: 241 HRKIAYGIEVGDGIPKMYKREVAEQALKNVGFDIEYEKDLADVNDEIPWYYPLAGEWKYV 300

[Close]

BLAST of HG10019409.1 vs. ExPASy TrEMBL
Match: A0A1S3CFL1 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103500326 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.2e-135
Identity = 252/326 (77.30%), Postives = 255/326 (78.22%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MSK GALDLASGLGGKLEK++VLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS
Sbjct: 1   MSKTGALDLASGLGGKLEKNDVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQL LKPGY                  
Sbjct: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLDLKPGYKVLDVGCGIGGPLREIAR 120

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA+  KTCDFVK                        
Sbjct: 121 FSYTSVTGLNNNEYQISRGKELNRVAKVDKTCDFVKADFMKMPFPDNSFDAVYAIEATCH 180

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
               YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK
Sbjct: 181 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           CLEALKQAGFEVVWEKDLA NSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVK LEFI
Sbjct: 241 CLEALKQAGFEVVWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKVLEFI 300

[Close]

BLAST of HG10019409.1 vs. ExPASy TrEMBL
Match: A0A5D3BJD2 (Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold474G00030 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.2e-135
Identity = 252/326 (77.30%), Postives = 255/326 (78.22%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MSK GALDLASGLGGKLEK++VLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS
Sbjct: 1   MSKTGALDLASGLGGKLEKNDVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQL LKPGY                  
Sbjct: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLDLKPGYKVLDVGCGIGGPLREIAR 120

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA+  KTCDFVK                        
Sbjct: 121 FSYTSVTGLNNNEYQISRGKELNRVAKVDKTCDFVKADFMKMPFPDNSFDAVYAIEATCH 180

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
               YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK
Sbjct: 181 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           CLEALKQAGFEVVWEKDLA NSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVK LEFI
Sbjct: 241 CLEALKQAGFEVVWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKVLEFI 300

[Close]

BLAST of HG10019409.1 vs. ExPASy TrEMBL
Match: A0A6J1DGP7 (Methyltransferase OS=Momordica charantia OX=3673 GN=LOC111020326 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 7.7e-133
Identity = 246/326 (75.46%), Postives = 253/326 (77.61%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MSKAGALDLASGLGGKL+K EVLSAVEKYEKYHVCYGGEEEERKANY+DMVNKYYDLVTS
Sbjct: 1   MSKAGALDLASGLGGKLDKGEVLSAVEKYEKYHVCYGGEEEERKANYSDMVNKYYDLVTS 60

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYEFGWGESFHFAPRW GESLRESIKRHEHFLALQLGLKPG                   
Sbjct: 61  FYEFGWGESFHFAPRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIAR 120

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA+   TCDFVK                        
Sbjct: 121 FSSTSVTGLNNNEYQISRGKELNRVAKVHNTCDFVKADFMKMPFPDNSFDAVYAIEATCH 180

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
               YGCYKEIYRVLKPGQHFAAYEWCMTD+FDPNNQEHQKIKAEIEIGDGLPDIR TGK
Sbjct: 181 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDSFDPNNQEHQKIKAEIEIGDGLPDIRSTGK 240

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           C+EALKQAGFEV+WEKDLA NSPLPWYLPLDKSHFSLSSFRLTA+GRFITKNMVKALEFI
Sbjct: 241 CIEALKQAGFEVIWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFI 300

[Close]

BLAST of HG10019409.1 vs. ExPASy TrEMBL
Match: A0A6J1F616 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111442447 PE=3 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.0e-132
Identity = 246/326 (75.46%), Postives = 254/326 (77.91%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MSKAGALDLASGLGGKL+K+EVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS
Sbjct: 42  MSKAGALDLASGLGGKLDKNEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 101

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYEFGWGESFHFAPRW GESLRESIKRHEHFLALQL LKPG+                  
Sbjct: 102 FYEFGWGESFHFAPRWNGESLRESIKRHEHFLALQLDLKPGHKVLDVGCGIGGPLREIAR 161

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA+  KTCDFVK                        
Sbjct: 162 FSYTSVTGLNNNEYQISRGKELNRVAKVDKTCDFVKADFMKMPFPDNSFDAVYAIEATCH 221

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
               YGCYKEIYRVLKPGQHFAAYEWCMTDAFD NNQEHQKIKAEIEIGDGLPDIRLTGK
Sbjct: 222 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDSNNQEHQKIKAEIEIGDGLPDIRLTGK 281

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           CLEALKQAGFEVVWE+DLA NSP+PWYLPLDKSHFSLSSFRLTA+GRFITKNMVK LEF+
Sbjct: 282 CLEALKQAGFEVVWERDLAVNSPVPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKVLEFV 341

[Close]

BLAST of HG10019409.1 vs. ExPASy TrEMBL
Match: A0A6J1FB93 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111442447 PE=3 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.0e-132
Identity = 246/326 (75.46%), Postives = 254/326 (77.91%), Query Frame = 0


Query: 1   MSKAGALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60
           MSKAGALDLASGLGGKL+K+EVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS
Sbjct: 1   MSKAGALDLASGLGGKLDKNEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------ 120
           FYEFGWGESFHFAPRW GESLRESIKRHEHFLALQL LKPG+                  
Sbjct: 61  FYEFGWGESFHFAPRWNGESLRESIKRHEHFLALQLDLKPGHKVLDVGCGIGGPLREIAR 120

Query: 121 -------------------KELNRVAEWAKTCDFVK------------------------ 180
                              KELNRVA+  KTCDFVK                        
Sbjct: 121 FSYTSVTGLNNNEYQISRGKELNRVAKVDKTCDFVKADFMKMPFPDNSFDAVYAIEATCH 180

Query: 181 ----YGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240
               YGCYKEIYRVLKPGQHFAAYEWCMTDAFD NNQEHQKIKAEIEIGDGLPDIRLTGK
Sbjct: 181 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDSNNQEHQKIKAEIEIGDGLPDIRLTGK 240

Query: 241 CLEALKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 262
           CLEALKQAGFEVVWE+DLA NSP+PWYLPLDKSHFSLSSFRLTA+GRFITKNMVK LEF+
Sbjct: 241 CLEALKQAGFEVVWERDLAVNSPVPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKVLEFV 300

[Close]

BLAST of HG10019409.1 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 425.6 bits (1093), Expect = 2.8e-119
Identity = 215/320 (67.19%), Postives = 239/320 (74.69%), Query Frame = 0


Query: 7   LDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGW 66
           +DLAS LGGK++KS+VL+AVEKYE+YHV +GG EEERKANYTDMVNKYYDL TSFYE+GW
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGW 60

Query: 67  GESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------------ 126
           GESFHFA RWKGESLRESIKRHEHFLALQLG++PG                         
Sbjct: 61  GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVV 120

Query: 127 -------------KELNRVAEWAKTCDFVK----------------------------YG 186
                        KELNR+A   KTC+FVK                            YG
Sbjct: 121 TGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYG 180

Query: 187 CYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGKCLEALK 246
           CYKEIYRVLKPGQ FAAYEWCMTDAFDP+N EHQKIK EIEIGDGLPDIRLT KCLEALK
Sbjct: 181 CYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALK 240

Query: 247 QAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFIRLAPKG 262
           QAGFEV+WEKDLA +SP+PWYLPLDK+HFSLSSFRLTA+GRFITKNMVK LE+IRLAP+G
Sbjct: 241 QAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQG 300

[Close]

BLAST of HG10019409.1 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 425.6 bits (1093), Expect = 2.8e-119
Identity = 215/320 (67.19%), Postives = 239/320 (74.69%), Query Frame = 0


Query: 7   LDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGW 66
           +DLAS LGGK++KS+VL+AVEKYE+YHV +GG EEERKANYTDMVNKYYDL TSFYE+GW
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGW 60

Query: 67  GESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGY------------------------ 126
           GESFHFA RWKGESLRESIKRHEHFLALQLG++PG                         
Sbjct: 61  GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVV 120

Query: 127 -------------KELNRVAEWAKTCDFVK----------------------------YG 186
                        KELNR+A   KTC+FVK                            YG
Sbjct: 121 TGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYG 180

Query: 187 CYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGKCLEALK 246
           CYKEIYRVLKPGQ FAAYEWCMTDAFDP+N EHQKIK EIEIGDGLPDIRLT KCLEALK
Sbjct: 181 CYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALK 240

Query: 247 QAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFIRLAPKG 262
           QAGFEV+WEKDLA +SP+PWYLPLDK+HFSLSSFRLTA+GRFITKNMVK LE+IRLAP+G
Sbjct: 241 QAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQG 300

[Close]

BLAST of HG10019409.1 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 122.9 bits (307), Expect = 3.9e-28
Identity = 92/322 (28.57%), Postives = 139/322 (43.17%), Query Frame = 0


Query: 6   ALDLASGLGGKLEKSEVLSAVEKYEKYHVCYGGEEE-ERKANYTDMVNKYYDLVTSFYEF 65
           A+DL+   GG +   +V    + Y++Y   +   +E E      D V+ +Y+LVT  YE+
Sbjct: 34  AVDLS---GGSISAEKV---QDNYKQYWSFFRRPKEIETAEKVPDFVDTFYNLVTDIYEW 93

Query: 66  GWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGYKEL------------------- 125
           GWG+SFHF+P   G+S +++ + HE      + +KPG K L                   
Sbjct: 94  GWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRA 153

Query: 126 ------------------NRVAEWAKTCDFV--------------------KYGC----- 185
                             N+ A     C+ V                    +  C     
Sbjct: 154 NVVGITINEYQVNRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKL 213

Query: 186 ---YKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGKCLEA 245
              Y EIYRVLKPG  + +YEW  T+ F   + EH ++   IE GD LP +R      E 
Sbjct: 214 EEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAET 273

Query: 246 LKQAGFEVVWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFIRLAP 262
            K+ GFE+V EKDLA+    PW+  L          ++  L  +    +V+ L  + +AP
Sbjct: 274 AKKVGFEIVKEKDLASPPAEPWWTRL----------KMGRLAYWRNHIVVQILSAVGVAP 333

[Close]

BLAST of HG10019409.1 vs. TAIR 10
Match: AT1G76090.1 (sterol methyltransferase 3 )

HSP 1 Score: 115.5 bits (288), Expect = 6.2e-26
Identity = 86/314 (27.39%), Postives = 131/314 (41.72%), Query Frame = 0


Query: 14  GGKLEKSEVLSAVEKYEKYHVCYGGEEE-ERKANYTDMVNKYYDLVTSFYEFGWGESFHF 73
           GG +   +V    + Y +Y   +   +E E      D V+ +Y+LVT  YE+GWG+SFHF
Sbjct: 39  GGSISAEKV---KDNYNQYWSFFRKPKEIESAEKVPDFVDTFYNLVTDIYEWGWGQSFHF 98

Query: 74  APRWKGESLRESIKRHEHFLALQLGLKPGYKEL--------------------------- 133
           +P   G+S +++ + HE      + +KPG K L                           
Sbjct: 99  SPHVPGKSDKDATRIHEEMAVDLIKVKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITIN 158

Query: 134 ----------NRVAEWAKTCDFV--------------------KYGC--------YKEIY 193
                     N+ A     C+ V                    +  C        Y EI+
Sbjct: 159 EYQVQRAKLHNKKAGLDSLCNVVCGNFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIF 218

Query: 194 RVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGKCLEALKQAGFEV 253
           RV+KPG  F +YEW  T+ +  +++EH+ +   IE GD LP +R         K+ GFEV
Sbjct: 219 RVMKPGSLFVSYEWVTTEKYRDDDEEHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEV 278

Query: 254 VWEKDLAANSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFIRLAPKGSQRVQD 262
           V EKDLA     PW+          +  ++  +  +    +V  L  I +APKG+  V  
Sbjct: 279 VKEKDLAKPPSKPWW----------NRLKMGRIAYWRNHVVVVILSAIGVAPKGTVDVHK 338

The following BLAST results are available for this feature:

BLAST of HG10019409.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903118.1	2.6e-135	76.38	cycloartenol-C-24-methyltransferase isoform X1 [Benincasa hispida] >XP_038903119...	[more]
XP_008461816.1	4.5e-135	77.30	PREDICTED: cycloartenol-C-24-methyltransferase [Cucumis melo] >XP_008461817.1 PR...	[more]
XP_004149721.1	4.5e-135	76.99	cycloartenol-C-24-methyltransferase [Cucumis sativus]	[more]
KAE8650927.1	4.5e-135	76.99	hypothetical protein Csa_000930 [Cucumis sativus]	[more]
KAA0048385.1	4.5e-135	77.30	cycloartenol-C-24-methyltransferase [Cucumis melo var. makuwa] >TYJ99862.1 cyclo...	[more]

BLAST of HG10019409.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LM02	4.0e-118	67.19	Cycloartenol-C-24-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=SMT1 PE=1...	[more]
Q6ZIX2	2.4e-107	60.74	Cycloartenol-C-24-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q54I98	2.5e-48	38.46	Probable cycloartenol-C-24-methyltransferase 1 OS=Dictyostelium discoideum OX=44...	[more]
O74198	1.0e-44	35.37	Sterol 24-C-methyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]
Q875K1	1.9e-43	37.16	Sterol 24-C-methyltransferase OS=Clavispora lusitaniae (strain ATCC 42720) OX=30...	[more]

BLAST of HG10019409.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CFL1	2.2e-135	77.30	Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103500326 PE=3 SV=1	[more]
A0A5D3BJD2	2.2e-135	77.30	Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold474G00...	[more]
A0A6J1DGP7	7.7e-133	75.46	Methyltransferase OS=Momordica charantia OX=3673 GN=LOC111020326 PE=3 SV=1	[more]
A0A6J1F616	1.0e-132	75.46	Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111442447 PE=3 SV=1	[more]
A0A6J1FB93	1.0e-132	75.46	Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111442447 PE=3 SV=1	[more]

BLAST of HG10019409.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G13710.1	2.8e-119	67.19	sterol methyltransferase 1	[more]
AT5G13710.2	2.8e-119	67.19	sterol methyltransferase 1	[more]
AT1G20330.1	3.9e-28	28.57	sterol methyltransferase 2	[more]
AT1G76090.1	6.2e-26	27.39	sterol methyltransferase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013705	Sterol methyltransferase C-terminal	PFAM	PF08498	Sterol_MT_C	coord: 213..261 e-value: 4.1E-18 score: 65.3
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 45..202 e-value: 4.1E-10 score: 41.5
None	No IPR available	PANTHER	PTHR44068	ZGC:194242	coord: 1..105 coord: 119..261
None	No IPR available	PANTHER	PTHR44068:SF3	METHYLTRANSFERASE	coord: 119..261
None	No IPR available	PANTHER	PTHR44068:SF3	METHYLTRANSFERASE	coord: 1..105
IPR030384	SAM-dependent methyltransferase SMT-type	PROSITE	PS51685	SAM_MT_ERG6_SMT	coord: 54..101 score: 21.652853
IPR030384	SAM-dependent methyltransferase SMT-type	PROSITE	PS51685	SAM_MT_ERG6_SMT	coord: 119..261 score: 48.496326
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 53..102
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 52..207

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10019409	HG10019409	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10019409.1-cds	HG10019409.1-cds-Chr04:21496580..21496681	CDS
HG10019409.1-cds	HG10019409.1-cds-Chr04:21496766..21496891	CDS
HG10019409.1-cds	HG10019409.1-cds-Chr04:21497003..21497086	CDS
HG10019409.1-cds	HG10019409.1-cds-Chr04:21497183..21497299	CDS
HG10019409.1-cds	HG10019409.1-cds-Chr04:21497668..21497715	CDS
HG10019409.1-cds	HG10019409.1-cds-Chr04:21498494..21498578	CDS
HG10019409.1-cds	HG10019409.1-cds-Chr04:21498677..21498750	CDS
HG10019409.1-cds	HG10019409.1-cds-Chr04:21498857..21498923	CDS
HG10019409.1-cds	HG10019409.1-cds-Chr04:21499388..21499470	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10019409.1	HG10019409.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006694	steroid biosynthetic process
biological_process	GO:0032259	methylation
biological_process	GO:0016126	sterol biosynthetic process
cellular_component	GO:0005783	endoplasmic reticulum
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003838	sterol 24-C-methyltransferase activity