HG10019397.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10019397.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	Chr04: 21369047 .. 21370102 (+)
Sequence length	1056
RNA-Seq Expression	HG10019397.1
Synteny	HG10019397.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGAAGAAATCATCAACTTCACCAAGGTCTGGTTAATCGTCTGCATCTCTCTCTGTTACTCCCACGCCGTCGGAAAACTCCTCCCTCCCGGCGCCGGCCGGTTCCTCGCCATTGCCCCCGTCGTCGGTCTCTTCTTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCGCCACCTCCTTCTTCGTCGCCTGGCTCGCAAACTTCAAGCTCCTCCTCTTCGCGGCCGGCGACGGCCCTCTCTCCACCACCCCGCCGCTCCCTCTCCGCCGATTCTTGGCTCTGTCTTGCTTGCCGATCAAGATCCCCCAAAACCCCTCGGAGAAATCGAGCCCTAATAATCGCACCAAAACCGTAAACGAAAAACCCAAATCGCTTCTTAACTACTCGATTAAAGCCCTAATCGTGGCATTGATGGTGAAATTGTACGATTACTCTGAGTTTATTCACCCGAAAGTTGTTTTATTCATTTATAGTTGGCACGTTTACTTTCTTCTTGAGATTATTCTGGCGATTACGCAGTTTTTCGGTCGGAATCTTCTCGGAATCGAGCTGGAGCCGCAGTTTCACGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAACCGACCGTTTACAAGCCGACGGTGAAGATCGCTTCTAAGGTAGTGGGGCGGATGTGGGCGCCGTTGCCGGCGGTGATGGCGACGTTTTTGGTGTCGGCAATGATGCACGAACTGATATTTTACTATTTGGGGCGAATGAGGCCCAACTGGGAGATAACGTGGTTCTTTGTAATCCATGGAATTGCTTTGACGGTGGAGATTCTGGTGAAGAAGGTGTTGGGTGAGAAGCTGCGGATACCGGCGGCAGTGTCGGTGCCGGTGACTCTGGCTTTCATCTTCTCCACTGCGATTTGGTTGTTTTTTCCACAGTTCGTGAGGTTGAAGTTGGATGTGAGGGCGTTTGAAGAATACGCCGCGTTGGTCAGTTTTGTGAAGAAAGTCGCTTTCTCATTGGTCGGTTGA

mRNA sequence

ATGGAGGAAGAAATCATCAACTTCACCAAGGTCTGGTTAATCGTCTGCATCTCTCTCTGTTACTCCCACGCCGTCGGAAAACTCCTCCCTCCCGGCGCCGGCCGGTTCCTCGCCATTGCCCCCGTCGTCGGTCTCTTCTTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCGCCACCTCCTTCTTCGTCGCCTGGCTCGCAAACTTCAAGCTCCTCCTCTTCGCGGCCGGCGACGGCCCTCTCTCCACCACCCCGCCGCTCCCTCTCCGCCGATTCTTGGCTCTGTCTTGCTTGCCGATCAAGATCCCCCAAAACCCCTCGGAGAAATCGAGCCCTAATAATCGCACCAAAACCGTAAACGAAAAACCCAAATCGCTTCTTAACTACTCGATTAAAGCCCTAATCGTGGCATTGATGGTGAAATTGTACGATTACTCTGAGTTTATTCACCCGAAAGTTGTTTTATTCATTTATAGTTGGCACGTTTACTTTCTTCTTGAGATTATTCTGGCGATTACGCAGTTTTTCGGTCGGAATCTTCTCGGAATCGAGCTGGAGCCGCAGTTTCACGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAACCGACCGTTTACAAGCCGACGGTGAAGATCGCTTCTAAGGTAGTGGGGCGGATGTGGGCGCCGTTGCCGGCGGTGATGGCGACGTTTTTGGTGTCGGCAATGATGCACGAACTGATATTTTACTATTTGGGGCGAATGAGGCCCAACTGGGAGATAACGTGGTTCTTTGTAATCCATGGAATTGCTTTGACGGTGGAGATTCTGGTGAAGAAGGTGTTGGGTGAGAAGCTGCGGATACCGGCGGCAGTGTCGGTGCCGGTGACTCTGGCTTTCATCTTCTCCACTGCGATTTGGTTGTTTTTTCCACAGTTCGTGAGGTTGAAGTTGGATGTGAGGGCGTTTGAAGAATACGCCGCGTTGGTCAGTTTTGTGAAGAAAGTCGCTTTCTCATTGGTCGGTTGA

Coding sequence (CDS)

ATGGAGGAAGAAATCATCAACTTCACCAAGGTCTGGTTAATCGTCTGCATCTCTCTCTGTTACTCCCACGCCGTCGGAAAACTCCTCCCTCCCGGCGCCGGCCGGTTCCTCGCCATTGCCCCCGTCGTCGGTCTCTTCTTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCGCCACCTCCTTCTTCGTCGCCTGGCTCGCAAACTTCAAGCTCCTCCTCTTCGCGGCCGGCGACGGCCCTCTCTCCACCACCCCGCCGCTCCCTCTCCGCCGATTCTTGGCTCTGTCTTGCTTGCCGATCAAGATCCCCCAAAACCCCTCGGAGAAATCGAGCCCTAATAATCGCACCAAAACCGTAAACGAAAAACCCAAATCGCTTCTTAACTACTCGATTAAAGCCCTAATCGTGGCATTGATGGTGAAATTGTACGATTACTCTGAGTTTATTCACCCGAAAGTTGTTTTATTCATTTATAGTTGGCACGTTTACTTTCTTCTTGAGATTATTCTGGCGATTACGCAGTTTTTCGGTCGGAATCTTCTCGGAATCGAGCTGGAGCCGCAGTTTCACGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAACCGACCGTTTACAAGCCGACGGTGAAGATCGCTTCTAAGGTAGTGGGGCGGATGTGGGCGCCGTTGCCGGCGGTGATGGCGACGTTTTTGGTGTCGGCAATGATGCACGAACTGATATTTTACTATTTGGGGCGAATGAGGCCCAACTGGGAGATAACGTGGTTCTTTGTAATCCATGGAATTGCTTTGACGGTGGAGATTCTGGTGAAGAAGGTGTTGGGTGAGAAGCTGCGGATACCGGCGGCAGTGTCGGTGCCGGTGACTCTGGCTTTCATCTTCTCCACTGCGATTTGGTTGTTTTTTCCACAGTTCGTGAGGTTGAAGTTGGATGTGAGGGCGTTTGAAGAATACGCCGCGTTGGTCAGTTTTGTGAAGAAAGTCGCTTTCTCATTGGTCGGTTGA

Protein sequence

MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGGATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTKTVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFGRNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPLPAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAAVSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLVG

Homology

[Close]

BLAST of HG10019397.1 vs. NCBI nr
Match: XP_023527814.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 611.3 bits (1575), Expect = 5.2e-171
Identity = 304/350 (86.86%), Postives = 327/350 (93.43%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           ME EIINFT VWLIVC+SLCY H  GK+LPPGAGRFLAIAPVV LFFLLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           AT+FF+AWLANFKLLLFAAG+GPLS TPPLPLRRFLALSCLPIKI QNPSEK SP N  +
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
           T+N++PKS LNY IKALIVALMVKLYDYSEFIHPKV+LFIYSWHVYF LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
           R LLGIELEPQFHDPY+STSLQDFWGRRWNLMATNILQPT+YKPTVKIA+KVVGR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAA 300
           P VMATFLVSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEILVKKVL EK R+PAA
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLV 351
           VSVPVTL FIFSTAIWLFFPQF+RLK+DV+AFEEYAALVSF+KK+AF LV
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of HG10019397.1 vs. NCBI nr
Match: XP_022982638.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 606.3 bits (1562), Expect = 1.7e-169
Identity = 303/350 (86.57%), Postives = 326/350 (93.14%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           ME EIINFT VWLIVC+SLCY H  GK+L PGAGRFLAI+PVV LFFLLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           AT+FF+AWLANFKLLLFAAG+GPLS TPPLPLRRFLALSCLPIKI QNPSEK SP N  +
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
           T+N++PKS LNY IKALIVALMVKLYDYSEFIHPKV+LFIYSWHVYF LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
           R LLGIELEPQFHDPY+STSLQDFWGRRWNLMATNILQPTVYKPTVKIA+KVVGR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAA 300
           P VMATFLVSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEILVKKVL EK R+PAA
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLV 351
           VSVPVTL FIFSTAIWLFFPQF+RLK+DV+AFEEYAALVSF+KK+AF LV
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLV 350

[Close]

BLAST of HG10019397.1 vs. NCBI nr
Match: XP_022934183.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 606.3 bits (1562), Expect = 1.7e-169
Identity = 300/350 (85.71%), Postives = 325/350 (92.86%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           ME E+INF  VWLIVC+SLCY H  GK+LPPGAGRFLAIAPVV LFFLLPLQLTS+HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           +T+FF+AWLANFKLLLF AG+GPLS TPPLPLRRFLALSCLPIKI QNPSEK SP N  +
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
           T+N++PKS LNY IKALIVALMVKLYDYSEFIHPKV+LFIYSWHVYF LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
           R LLGIELEPQFHDPY+STSLQDFWGRRWNLMATNILQPT+YKPTVKIA+KVVGR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAA 300
           P VMATFLVSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEILVKKVL EK R+PAA
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLV 351
           VSVPVTL FIFSTAIWLFFPQF+RLK+DV+AFEEYAALVSF+KK+AF LV
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of HG10019397.1 vs. NCBI nr
Match: XP_022948970.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 599.7 bits (1545), Expect = 1.6e-167
Identity = 304/351 (86.61%), Postives = 321/351 (91.45%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           ME EI+NF KVWL VCISLCY HA GKLL PG GRFLA+AP+V LFFLLPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           +TSFF+AWLANFKLLLFAAGDGPLS  PPLPLRRFLALSCLPIKI QN  EKS PNN   
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
               KPKS LNYSIKA+IVALMVKLYDYSEFIHPKVVLFIYSWH+YFLLEIILAITQFFG
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
           RN+LGIELEPQF DPY+STSLQDFWGRRWNLMATNILQPTVYKP+VKIA+KV+GR WAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAA 300
           PAVMATFLVSAMMHELIFYY+GRMRPNWE TWFFVIHG+ALTVEI+VKKVL EK R+P A
Sbjct: 241 PAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLVG 352
           VSVPVTLAFIF TAIWLFFPQFVRLKLDVRAFEEYAALV+FVK VAFSLVG
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of HG10019397.1 vs. NCBI nr
Match: XP_022998466.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 599.4 bits (1544), Expect = 2.1e-167
Identity = 302/351 (86.04%), Postives = 321/351 (91.45%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           ME EI+NF KVWL VCISLCY HA GKL+PPGAGRFLA+AP+V LFF LPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           +TSFF+AWLANFKLLLF AGDGPLS  PPLPLRRFLALSCLPIKI QN  EKS PNN   
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
               KPKS LNYSIKA+IVALMVKLYDYSEFIHPKVVLFIYSWH+YFLLEIILAITQFFG
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
           RN+LGIELEPQF DPY+STSLQDFWGRRWNLMATNILQPTVYKP+VKIA+KV+GR WAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAA 300
           PAVMATFLVSAMMHELIF+Y+GRMRPNWE TWFFVIHG+ALTVEI+VKKVL EK R+P A
Sbjct: 241 PAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLVG 352
           VSVPVTLAFIF TAIWLFFPQFVRLKLDVRAFEEYAALV+FVK VAFSLVG
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of HG10019397.1 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-85
Identity = 170/340 (50.00%), Postives = 236/340 (69.41%), Query Frame = 0


Query: 7   NFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGGATSFFV 66
           +F K W +V ISLCY+  + KL+P G  R +   PV  +FF++P  + S+HL G T+FF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFAAGDGPLSTT-PPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTKTVNEK 126
           AWLANFKLLLFA G GPLS+   PL L  FLA+SCLPIKI  +P    + ++   T  E 
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGST--EG 122

Query: 127 PKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFGRNLLG 186
           P   L Y+IKA+ V L++K Y+YS  +  KVVL +Y+ H+YF LEIILA T    R +  
Sbjct: 123 P---LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSD 182

Query: 187 IELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPLPAVMA 246
           +ELEPQF+ PY +TSLQDFWGRRWNLM T IL+PTVY+P++++ S V+G  ++ + A   
Sbjct: 183 LELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFG 242

Query: 247 TFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAAVSVPV 306
           TF+VS +MHELIF+Y+GR+RP+W++ WFF+I+G   TVEI +KK +  + R P A+S  +
Sbjct: 243 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 302

Query: 307 TLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKV 346
           TL F+  TA+WLF P+F R  +  +A +EYAA+ +F  +V
Sbjct: 303 TLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

[Close]

BLAST of HG10019397.1 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 5.4e-70
Identity = 145/352 (41.19%), Postives = 219/352 (62.22%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLP-PGAGRFLAIAPVVGLFFLLPLQLTSIHLG 60
           +E+E+  F++VW+    + CY   V  + P  GA R L + PVV LF  LPL+L+S HLG
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRT 120
           G T+ ++ WLANFKLLLFA   GPLS  P L L  F++ + LPIK   +PS     N R 
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPLS-NPSLSLLHFISTTLLPIKFRDDPSNDHEKNKR- 122

Query: 121 KTVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFF 180
            T++ + + ++ +  K +  A ++K+Y++ + +   V+  +Y +H Y   EI LA +   
Sbjct: 123 -TLSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVI 182

Query: 181 GRNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAP 240
            R  LG++L PQF++PY +TSLQDFWGRRWNLM ++IL  T Y+P  ++ S+ V   W  
Sbjct: 183 ARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEV 242

Query: 241 LPAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPA 300
             A++  F VS +MHE+ F+YL R RP+WE+T FFV+HG+   VE++VKK +  K+R+  
Sbjct: 243 AGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRR 302

Query: 301 AVSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLVG 352
            VS  +T+ F+  T  WLF PQ VR  +D++  +EY  + ++ +K    L+G
Sbjct: 303 EVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLLG 351

[Close]

BLAST of HG10019397.1 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 3.9e-68
Identity = 145/346 (41.91%), Postives = 209/346 (60.40%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           M+EE+ N  KVW+   IS+ Y + +   +  GA RFL+++PV+ LF +LPL  +S+HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
            T+FF+ WLANFKL+LF+   GPL    P    RF   +C PIK+ QNP          K
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNP----------K 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
           + N  PK  L ++IK  I A+++ LY Y + + P ++L +Y  H+Y  +EIIL   +   
Sbjct: 121 SQNHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
              LG +LEPQ + PY +TSLQDFWGRRWNLM   IL+P VY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKK--VLGEKLRIP 300
           P ++A F+VS ++HEL+F+YL R  P  E+T FFV+HG+   VE+ VKK   + ++ R+ 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 AAVSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKK 345
             VS  +T+ F+F T  WLF PQ  R  +  R   E   LV F+K+
Sbjct: 301 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of HG10019397.1 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-63
Identity = 139/350 (39.71%), Postives = 202/350 (57.71%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           MEEE+++  KVW+   IS+ Y +     +  G  R L++ PV  LF +LPL ++S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           +T+FF++WLANFKL+LF+   GPL   P   L RF+  +C PIK+ QNP          K
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNP----------K 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
             N+ PK    +++K     +++ +Y+Y + + P V+L +YS H+Y   EI+LA  +   
Sbjct: 121 PQNQMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLL 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
              L  +LEP F++PY STSLQDFWGRRWNLM   IL+P VY P  ++A + +    A  
Sbjct: 181 SISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKV-LGEKLRIPA 300
             V A+FLVS ++HELIF+Y  R  P  E+T FFV+HG+    E   K+  L  + ++  
Sbjct: 241 LGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQ 300

Query: 301 AVSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSL 350
            VS  +T+ F+  T  WLFFP   R  +  R  +E    + FVK   F L
Sbjct: 301 MVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of HG10019397.1 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 6.4e-63
Identity = 141/344 (40.99%), Postives = 205/344 (59.59%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           MEE+  N  +VW+   ISL Y + +   L  G  R L+I PV  LF +LPL L+ +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
            +  F++WLANFKLLLFA  +GPL   PP  L RF+  +CLPIKI Q+PS  + PN   K
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPNLHPK 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
            +   PK +L  ++K L++ +++ +Y+Y + +   VVL +Y  H+Y  +E++L       
Sbjct: 121 PM---PKWVL--AVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVV 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
             LLG  +EP F++PY +TSLQDFW RRWNLM + +L+ TV+ P  +   +++    A  
Sbjct: 181 STLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIP-A 300
             VMA+F VS +MHEL+++Y+ R  P WE+T FFV+HG A   EI VK+   + LR P  
Sbjct: 241 AGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT--QWLRPPHR 300

Query: 301 AVSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVK 344
           AVS  V L F+  T +WLF  Q +R  +  +A  E   ++   K
Sbjct: 301 AVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

[Close]

BLAST of HG10019397.1 vs. ExPASy TrEMBL
Match: A0A6J1J5C4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481453 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.1e-170
Identity = 303/350 (86.57%), Postives = 326/350 (93.14%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           ME EIINFT VWLIVC+SLCY H  GK+L PGAGRFLAI+PVV LFFLLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           AT+FF+AWLANFKLLLFAAG+GPLS TPPLPLRRFLALSCLPIKI QNPSEK SP N  +
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
           T+N++PKS LNY IKALIVALMVKLYDYSEFIHPKV+LFIYSWHVYF LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
           R LLGIELEPQFHDPY+STSLQDFWGRRWNLMATNILQPTVYKPTVKIA+KVVGR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAA 300
           P VMATFLVSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEILVKKVL EK R+PAA
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLV 351
           VSVPVTL FIFSTAIWLFFPQF+RLK+DV+AFEEYAALVSF+KK+AF LV
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLV 350

[Close]

BLAST of HG10019397.1 vs. ExPASy TrEMBL
Match: A0A6J1F6Y6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441427 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.1e-170
Identity = 300/350 (85.71%), Postives = 325/350 (92.86%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           ME E+INF  VWLIVC+SLCY H  GK+LPPGAGRFLAIAPVV LFFLLPLQLTS+HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           +T+FF+AWLANFKLLLF AG+GPLS TPPLPLRRFLALSCLPIKI QNPSEK SP N  +
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
           T+N++PKS LNY IKALIVALMVKLYDYSEFIHPKV+LFIYSWHVYF LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
           R LLGIELEPQFHDPY+STSLQDFWGRRWNLMATNILQPT+YKPTVKIA+KVVGR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAA 300
           P VMATFLVSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEILVKKVL EK R+PAA
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLV 351
           VSVPVTL FIFSTAIWLFFPQF+RLK+DV+AFEEYAALVSF+KK+AF LV
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of HG10019397.1 vs. ExPASy TrEMBL
Match: A0A6J1GBE5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452458 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.6e-168
Identity = 304/351 (86.61%), Postives = 321/351 (91.45%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           ME EI+NF KVWL VCISLCY HA GKLL PG GRFLA+AP+V LFFLLPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           +TSFF+AWLANFKLLLFAAGDGPLS  PPLPLRRFLALSCLPIKI QN  EKS PNN   
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
               KPKS LNYSIKA+IVALMVKLYDYSEFIHPKVVLFIYSWH+YFLLEIILAITQFFG
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
           RN+LGIELEPQF DPY+STSLQDFWGRRWNLMATNILQPTVYKP+VKIA+KV+GR WAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAA 300
           PAVMATFLVSAMMHELIFYY+GRMRPNWE TWFFVIHG+ALTVEI+VKKVL EK R+P A
Sbjct: 241 PAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLVG 352
           VSVPVTLAFIF TAIWLFFPQFVRLKLDVRAFEEYAALV+FVK VAFSLVG
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of HG10019397.1 vs. ExPASy TrEMBL
Match: A0A6J1KAA2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493091 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.9e-168
Identity = 302/351 (86.04%), Postives = 321/351 (91.45%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           ME EI+NF KVWL VCISLCY HA GKL+PPGAGRFLA+AP+V LFF LPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           +TSFF+AWLANFKLLLF AGDGPLS  PPLPLRRFLALSCLPIKI QN  EKS PNN   
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
               KPKS LNYSIKA+IVALMVKLYDYSEFIHPKVVLFIYSWH+YFLLEIILAITQFFG
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
           RN+LGIELEPQF DPY+STSLQDFWGRRWNLMATNILQPTVYKP+VKIA+KV+GR WAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAA 300
           PAVMATFLVSAMMHELIF+Y+GRMRPNWE TWFFVIHG+ALTVEI+VKKVL EK R+P A
Sbjct: 241 PAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLVG 352
           VSVPVTLAFIF TAIWLFFPQFVRLKLDVRAFEEYAALV+FVK VAFSLVG
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of HG10019397.1 vs. ExPASy TrEMBL
Match: A0A0A0L9S2 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 3.9e-164
Identity = 299/352 (84.94%), Postives = 317/352 (90.06%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           ME EIIN TKVWL V ISL Y HA  KLL PG  RFLAIAPVV +FFLLPLQLTSIH GG
Sbjct: 28  MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 87

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSP-NNRT 120
           AT FFV WLA FKLLL  AGDGPLSTTPPLPL RF+A+SCLPIKI +NPSEKS+P NNR 
Sbjct: 88  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 147

Query: 121 KTVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFF 180
           +T+NEKPKS LNYSI+ALIV LMVKLYDYS F HPK+VLFIYSWHVY LLEIILAITQFF
Sbjct: 148 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 207

Query: 181 GRNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAP 240
           GRNLLGIELEPQF DPYYSTSLQDFWGRRWNLMATNILQP VYKPTVKIA++V+GRMWAP
Sbjct: 208 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 267

Query: 241 LPAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPA 300
           LP VMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEI++KK+L EK R+PA
Sbjct: 268 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 327

Query: 301 AVSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSLVG 352
            VSVPVTL FIFSTAIWLFFPQFVRL+LDVRAFEEYAAL +FVKKVAFSLVG
Sbjct: 328 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLVG 379

[Close]

BLAST of HG10019397.1 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 318.2 bits (814), Expect = 8.5e-87
Identity = 170/340 (50.00%), Postives = 236/340 (69.41%), Query Frame = 0


Query: 7   NFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGGATSFFV 66
           +F K W +V ISLCY+  + KL+P G  R +   PV  +FF++P  + S+HL G T+FF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFAAGDGPLSTT-PPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTKTVNEK 126
           AWLANFKLLLFA G GPLS+   PL L  FLA+SCLPIKI  +P    + ++   T  E 
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGST--EG 122

Query: 127 PKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFGRNLLG 186
           P   L Y+IKA+ V L++K Y+YS  +  KVVL +Y+ H+YF LEIILA T    R +  
Sbjct: 123 P---LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSD 182

Query: 187 IELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPLPAVMA 246
           +ELEPQF+ PY +TSLQDFWGRRWNLM T IL+PTVY+P++++ S V+G  ++ + A   
Sbjct: 183 LELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFG 242

Query: 247 TFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIPAAVSVPV 306
           TF+VS +MHELIF+Y+GR+RP+W++ WFF+I+G   TVEI +KK +  + R P A+S  +
Sbjct: 243 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 302

Query: 307 TLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKV 346
           TL F+  TA+WLF P+F R  +  +A +EYAA+ +F  +V
Sbjct: 303 TLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

[Close]

BLAST of HG10019397.1 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.8e-69
Identity = 145/346 (41.91%), Postives = 209/346 (60.40%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           M+EE+ N  KVW+   IS+ Y + +   +  GA RFL+++PV+ LF +LPL  +S+HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
            T+FF+ WLANFKL+LF+   GPL    P    RF   +C PIK+ QNP          K
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNP----------K 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
           + N  PK  L ++IK  I A+++ LY Y + + P ++L +Y  H+Y  +EIIL   +   
Sbjct: 121 SQNHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
              LG +LEPQ + PY +TSLQDFWGRRWNLM   IL+P VY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKK--VLGEKLRIP 300
           P ++A F+VS ++HEL+F+YL R  P  E+T FFV+HG+   VE+ VKK   + ++ R+ 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 AAVSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKK 345
             VS  +T+ F+F T  WLF PQ  R  +  R   E   LV F+K+
Sbjct: 301 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of HG10019397.1 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.6e-64
Identity = 139/350 (39.71%), Postives = 202/350 (57.71%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           MEEE+++  KVW+   IS+ Y +     +  G  R L++ PV  LF +LPL ++S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
           +T+FF++WLANFKL+LF+   GPL   P   L RF+  +C PIK+ QNP          K
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNP----------K 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
             N+ PK    +++K     +++ +Y+Y + + P V+L +YS H+Y   EI+LA  +   
Sbjct: 121 PQNQMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLL 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
              L  +LEP F++PY STSLQDFWGRRWNLM   IL+P VY P  ++A + +    A  
Sbjct: 181 SISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKV-LGEKLRIPA 300
             V A+FLVS ++HELIF+Y  R  P  E+T FFV+HG+    E   K+  L  + ++  
Sbjct: 241 LGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQ 300

Query: 301 AVSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVKKVAFSL 350
            VS  +T+ F+  T  WLFFP   R  +  R  +E    + FVK   F L
Sbjct: 301 MVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of HG10019397.1 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 242.7 bits (618), Expect = 4.5e-64
Identity = 141/344 (40.99%), Postives = 205/344 (59.59%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           MEE+  N  +VW+   ISL Y + +   L  G  R L+I PV  LF +LPL L+ +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKIPQNPSEKSSPNNRTK 120
            +  F++WLANFKLLLFA  +GPL   PP  L RF+  +CLPIKI Q+PS  + PN   K
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPNLHPK 120

Query: 121 TVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQFFG 180
            +   PK +L  ++K L++ +++ +Y+Y + +   VVL +Y  H+Y  +E++L       
Sbjct: 121 PM---PKWVL--AVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVV 180

Query: 181 RNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWAPL 240
             LLG  +EP F++PY +TSLQDFW RRWNLM + +L+ TV+ P  +   +++    A  
Sbjct: 181 STLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKKVLGEKLRIP-A 300
             VMA+F VS +MHEL+++Y+ R  P WE+T FFV+HG A   EI VK+   + LR P  
Sbjct: 241 AGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT--QWLRPPHR 300

Query: 301 AVSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVK 344
           AVS  V L F+  T +WLF  Q +R  +  +A  E   ++   K
Sbjct: 301 AVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

[Close]

BLAST of HG10019397.1 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 235.3 bits (599), Expect = 7.3e-62
Identity = 133/347 (38.33%), Postives = 201/347 (57.93%), Query Frame = 0


Query: 1   MEEEIINFTKVWLIVCISLCYSHAVGKLLPPGAGRFLAIAPVVGLFFLLPLQLTSIHLGG 60
           MEEE+ NF K+W+   IS+ Y + +   +  G  R L++ PV  LF + PL  + +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  ATSFFVAWLANFKLLLFAAGDGPLSTTPPLP--LRRFLALSCLPIKIPQNPSEKSSPNNR 120
             +FF++WLANFKL+LF+   GPLS   PLP  L RF+ ++C PIK  QNP+        
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNPN-------- 120

Query: 121 TKTVNEKPKSLLNYSIKALIVALMVKLYDYSEFIHPKVVLFIYSWHVYFLLEIILAITQF 180
              +      +  ++IK +I  +++++Y+Y +++ P ++L   S H++  LEI+  + + 
Sbjct: 121 ---IQNYKIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKA 180

Query: 181 FGRNLLGIELEPQFHDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVVGRMWA 240
                LG +LEPQ ++PY +TSLQDFWGRRWNLM   IL+P VY P  ++A + V    A
Sbjct: 181 LVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQA 240

Query: 241 PLPAVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEILVKK--VLGEKLR 300
               V A FLVS  +HE++F+YL R  P  E+TWFF++HG+    E+ VKK   +    R
Sbjct: 241 MFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWR 300

Query: 301 IPAAVSVPVTLAFIFSTAIWLFFPQFVRLKLDVRAFEEYAALVSFVK 344
           +   VS  +T+ F+  T+ W FFP  +R  +  R   E    + FVK
Sbjct: 301 VSPTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

The following BLAST results are available for this feature:

BLAST of HG10019397.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023527814.1	5.2e-171	86.86	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982638.1	1.7e-169	86.57	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_022934183.1	1.7e-169	85.71	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]
XP_022948970.1	1.6e-167	86.61	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acy...	[more]
XP_022998466.1	2.1e-167	86.04	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]

BLAST of HG10019397.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	1.2e-85	50.00	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	5.4e-70	41.19	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ76	3.9e-68	41.91	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ75	2.2e-63	39.71	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	6.4e-63	40.99	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]

BLAST of HG10019397.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J5C4	8.1e-170	86.57	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F6Y6	8.1e-170	85.71	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1GBE5	7.6e-168	86.61	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KAA2	9.9e-168	86.04	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A0A0L9S2	3.9e-164	84.94	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=...	[more]

BLAST of HG10019397.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	8.5e-87	50.00	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	2.8e-69	41.91	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	1.6e-64	39.71	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	4.5e-64	40.99	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	7.3e-62	38.33	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 189..275 e-value: 3.7E-16 score: 59.2
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..351 e-value: 2.6E-118 score: 393.1
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 2..346
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..346

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10019397	HG10019397	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10019397.1-cds	HG10019397.1-cds-Chr04:21369047..21370102	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10019397.1	HG10019397.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity