HG10019395.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10019395.1 vs. NCBI nr
Match: XP_038905685.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 604.7 bits (1558), Expect = 4.9e-169
Identity = 310/355 (87.32%), Postives = 325/355 (91.55%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           MAAEIRIFLQTLFLVSSSLCFCF+IRNSIPNGF KF LILPFFFLFLYIPLQFQ+IH QG
Sbjct: 1   MAAEIRIFLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQSIHFQG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
           SIGFFIGWL SFKLLLFAFGKGPLCS AASSSL  FLAVGSLPIEIPDH+PA N S   P
Sbjct: 61  SIGFFIGWLGSFKLLLFAFGKGPLCSAAASSSLRHFLAVGSLPIEIPDHKPAANHSHSNP 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGV 180
           VLPLSFS+KLGLLILT  AIYFKN  H NA LIFFC VIYLLLEIL+GGAAAL KA LGV
Sbjct: 121 VLPLSFSSKLGLLILTIVAIYFKNLFHPNAILIFFCFVIYLLLEILLGGAAALAKATLGV 180

Query: 181 DLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTAT 240
           +LLPYFDEPYLSDSLQDFWGRRWNLMTSRILRL VYDPCRK+T+GIVGRKPAAM+A+TAT
Sbjct: 181 ELLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLVVYDPCRKITIGIVGRKPAAMMAMTAT 240

Query: 241 FAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLT 300
           FAVSG+MHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEM IKSAVGGRFRLPG+VSA LT
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSAVGGRFRLPGVVSAALT 300

Query: 301 IGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQLITPLKFISFQLK 356
           IGFVMTTSFWLFFPQFL+ K+DVRMFEE+AALGAFVKSVT QLITPL  ISF  K
Sbjct: 301 IGFVMTTSFWLFFPQFLKLKSDVRMFEEYAALGAFVKSVTGQLITPLN-ISFHFK 354

BLAST of HG10019395.1 vs. NCBI nr
Match: XP_022982637.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 555.8 bits (1431), Expect = 2.6e-154
Identity = 282/355 (79.44%), Postives = 314/355 (88.45%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           MAAEI  F+QTLFL+SSSLC CFSIRNSIPNGF KF  +LPFFFLFLYIPLQFQTIHL+G
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
           ++GFFIGWL SFKLLLFAFGKGPLCS A SSSL RFLAV SLPI+IP+H+P  NRSDP P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGV 180
           +LPLSF+AKLG LILTFAAIYFKN  + N  LIFFCL+IYLLLEILIGGA A+ +A LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 DLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTAT 240
           +LLP+FDEPYLSDSLQDFWGRRWNL++SRILR AV+DPCRKL   +VG+KPA +IAV AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLT 300
           FAVSGVMHELIYFYMGRLAPTWEV+ FFL+HGVCLAAEM +KSAVG RFR P +VS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 IGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQLITPLKFISFQLK 356
           +GFVM TSFWLFFPQ LRFKADVRM EE+AALGAFVK+VTA+LI PL FISFQ+K
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGPL-FISFQVK 354

BLAST of HG10019395.1 vs. NCBI nr
Match: XP_022934181.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6581558.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 553.1 bits (1424), Expect = 1.7e-153
Identity = 282/355 (79.44%), Postives = 312/355 (87.89%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           MAAEI  F+QTLFL++SSLC CFSIRNSIPNGF KF  +LPFFFLFLYIPLQFQTIHL+G
Sbjct: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
           ++GFFIGWL SFKLLLFAFGKGPLC  A SSSL RFLAV  LPI+IP+H+   NRSDP P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGV 180
           +LPLSF+AKLG LILTFAAIYFKN  + N  LIFFCL+IYLLLEILIGGA AL +A LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 DLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTAT 240
           +LLP+FDEPYLSDSLQDFWGRRWNL++SRILR AV+DPCRKL   +VG+KPAA+IAV AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240

Query: 241 FAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLT 300
           FAVSGVMHELIYFYMGRL PTWEV+ FFL+HGVCLAAEM +KSAVGGRFR P +VS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300

Query: 301 IGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQLITPLKFISFQLK 356
           IGFVM TSFWLFFPQ LRFKADVRM EE+AALGAFVKSVTA+LI PL FISFQ+K
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL-FISFQVK 354

BLAST of HG10019395.1 vs. NCBI nr
Match: KAG7018062.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 553.1 bits (1424), Expect = 1.7e-153
Identity = 282/355 (79.44%), Postives = 312/355 (87.89%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           MAAEI  F+QTLFL++SSLC CFSIRNSIPNGF KF  +LPFFFLFLYIPLQFQTIHL+G
Sbjct: 188 MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 247

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
           ++GFFIGWL SFKLLLFAFGKGPLC  A SSSL RFLAV  LPI+IP+H+   NRSDP P
Sbjct: 248 TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 307

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGV 180
           +LPLSF+AKLG LILTFAAIYFKN  + N  LIFFCL+IYLLLEILIGGA AL +A LGV
Sbjct: 308 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 367

Query: 181 DLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTAT 240
           +LLP+FDEPYLSDSLQDFWGRRWNL++SRILR AV+DPCRKL   +VG+KPAA+IAV AT
Sbjct: 368 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 427

Query: 241 FAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLT 300
           FAVSGVMHELIYFYMGRL PTWEV+ FFL+HGVCLAAEM +KSAVGGRFR P +VS  LT
Sbjct: 428 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 487

Query: 301 IGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQLITPLKFISFQLK 356
           IGFVM TSFWLFFPQ LRFKADVRM EE+AALGAFVKSVTA+LI PL FISFQ+K
Sbjct: 488 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL-FISFQVK 541

BLAST of HG10019395.1 vs. NCBI nr
Match: XP_023528035.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 552.4 bits (1422), Expect = 2.9e-153
Identity = 280/355 (78.87%), Postives = 312/355 (87.89%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           MAAEI  F+QTLFL+S+SLC CFSIRNSIPNGF KF  +LPFFFLFLYIPLQFQTIHL+G
Sbjct: 1   MAAEIHAFIQTLFLISTSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
           ++GFFIGWL SFKLLLFAFGKGPLCS A SSSL RFLAV SLPI+IP+H+P  +RSDP P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPDRSDPDP 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGV 180
           +LPLSF+AKLG LILTFAAIYFKN  + N  LI FCL+IYLLLEILIGGA AL +A LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLILFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 DLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTAT 240
           + LP+FDEPYLSDSLQDFWGRRWNL++SRILR AV+DPCRKL    VG+KPAA+IAV AT
Sbjct: 181 EFLPFFDEPYLSDSLQDFWGRRWNLVSSRILRSAVFDPCRKLAGTFVGKKPAAIIAVMAT 240

Query: 241 FAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLT 300
           FAVSGVMHELIYFYMGRL PTWEV+ FFL+HGVCLAAEM +KSAVGGRFR P +VS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGLLT 300

Query: 301 IGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQLITPLKFISFQLK 356
           IGFVM TSFWLFFPQ LRFKADVRM EE+A+LGAFVKSVTA++I PL FISFQ+K
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYASLGAFVKSVTAEMIAPL-FISFQVK 354

BLAST of HG10019395.1 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.7e-68
Identity = 145/338 (42.90%), Postives = 200/338 (59.17%), Query Frame = 0

Query: 8   FLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQGSIGFFIG 67
           F++   LV  SLC+ F I   +P G  +  L  P F +F  +P    ++HL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIP-DHEPAKNRSDPGPVL-PLS 127
           WLA+FKLLLFA G+GPL S     SL  FLAV  LPI+I    +P K  S  G    PL 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 FSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGVDLLPY 187
           ++ K   ++L   A  +   L +   L  + + IY  LEI++   AA V+A   ++L P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 188 FDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTATFAVSG 247
           F++PYL+ SLQDFWGRRWNLM + ILR  VY+P  +L   ++G   + ++A   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 248 VMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLTIGFVM 307
           +MHELI+FYMGRL P W++  FFL++G C   E+ IK  + GR+R P  +S  LT+ FVM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 TTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQL 344
            T+ WLF P+F R     +  +E+AA+GAF   V  +L
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKL 340

BLAST of HG10019395.1 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 8.2e-58
Identity = 134/335 (40.00%), Postives = 186/335 (55.52%), Query Frame = 0

Query: 4   EIRIFLQTLFLVSSSLCFCFSIRNSIPN-GFFKFFLILPFFFLFLYIPLQFQTIHLQGSI 63
           E++ F +      ++ C+C  +    P+ G  +  L+LP   LF+++PL+  + HL G  
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 64  GFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEI-----PDHEPAKNRSD 123
             ++ WLA+FKLLLFAF  GPL +   S SL  F++   LPI+       DHE  K    
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPLSN--PSLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLS 125

Query: 124 PGPVLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAA 183
                 + F AKL           F+  L      + +C   YL  EI +  +A + +A 
Sbjct: 126 FEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARAT 185

Query: 184 LGVDLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAV 243
           LG+DL P F+EPYL+ SLQDFWGRRWNLM S IL L  Y P R++    V  +     A+
Sbjct: 186 LGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGAM 245

Query: 244 TATFAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSA 303
              F VSG+MHE+ +FY+ R  P+WEVT FF+LHGVC A EM +K AV G+ RL   VS 
Sbjct: 246 LVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVSG 305

Query: 304 PLTIGFVMTTSFWLFFPQFLRFKADVRMFEEHAAL 333
            LT+GFVM T  WLF PQ +R   D++  +E+  +
Sbjct: 306 ALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVM 338

BLAST of HG10019395.1 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 7.7e-56
Identity = 134/338 (39.64%), Postives = 189/338 (55.92%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           M  E+   ++       S+ +C+     I +G F+   +LP   LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
           S  FF+ WLA+FKL+LF+F +GPL      S+L RF+     PI++  +   +N+     
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKPQNQ----- 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGV 180
           +    F+ KL    +      +K H+     L+ + L IYL  EIL+     L+  +L  
Sbjct: 121 MPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWC 180

Query: 181 DLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTAT 240
           DL P+F+EPYLS SLQDFWGRRWNLM   ILR AVY P R++    +    A  + V A+
Sbjct: 181 DLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFAS 240

Query: 241 FAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIK-SAVGGRFRLPGIVSAPL 300
           F VSGV+HELI+FY  R +PT EVT FF+LHGVC AAE   K + +  R+++  +VS  L
Sbjct: 241 FLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLL 300

Query: 301 TIGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVK 338
           T+GFV+ T  WLFFP   R     R+ +E      FVK
Sbjct: 301 TVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

BLAST of HG10019395.1 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.2e-53
Identity = 129/317 (40.69%), Postives = 182/317 (57.41%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           M  E++ F++       S+ +C+ +   I  G F+   +LP   LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
            + FF+ WLA+FKL+LF+F +GPL  +    +L RF+ +   PI     +P +N +    
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPI-----KPQQNPNIQNY 120

Query: 121 VLPL-SFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALG 180
            +P+  F+ K+ + ++      +K +L     L+F  L I+L LEI+     ALV   LG
Sbjct: 121 KIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLG 180

Query: 181 VDLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTA 240
            DL P  +EPYL+ SLQDFWGRRWNLM   ILR AVY P R++    V    A  + V A
Sbjct: 181 CDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFA 240

Query: 241 TFAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGR--FRLPGIVSA 300
            F VSG +HE+++FY+ R  PT EVT FFLLHGVC  AE+ +K +   R  +R+   VS 
Sbjct: 241 AFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSR 300

Query: 301 PLTIGFVMTTSFWLFFP 315
            LT+GFV+ TS W FFP
Sbjct: 301 LLTVGFVVVTSGWFFFP 310

BLAST of HG10019395.1 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.3e-51
Identity = 126/332 (37.95%), Postives = 186/332 (56.02%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           M  E+R  ++       S+ +C+ I + I  G  +   +LP F +FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
             GFF  WLA+FKL LFAF + PL  +   S+L RF      PI+I +  P+ NR    P
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKI-NKNPSSNRIHNKP 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNH----LHQNAYLIFFCLVIYLLLEILIGGAAALVKA 180
           +     + K  LLI +F    ++N+    L + A+L  F + +YL  E+++    AL+  
Sbjct: 121 MSKWVLAFK--LLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSM 180

Query: 181 ALGVDLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIA 240
            LG ++ P F++PYL+ SLQ+FW RRWNLM   +LR AV+ P ++    ++G   A    
Sbjct: 181 LLGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAG 240

Query: 241 VTATFAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVS 300
           + ATF VSG+MHELIYFY+ R +PTWEVTCFFLLHGV    E+ +K           +  
Sbjct: 241 MLATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKRMRWLPTPRRAVSG 300

Query: 301 APLTIGFVMTTSFWLFFPQFLRFKADVRMFEE 329
             +T+ F++ T+ WLF+PQ LR     R+  E
Sbjct: 301 LAITV-FLLVTAGWLFYPQMLRNDVHKRVISE 326

BLAST of HG10019395.1 vs. ExPASy TrEMBL
Match: A0A6J1IX47 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481451 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.3e-154
Identity = 282/355 (79.44%), Postives = 314/355 (88.45%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           MAAEI  F+QTLFL+SSSLC CFSIRNSIPNGF KF  +LPFFFLFLYIPLQFQTIHL+G
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
           ++GFFIGWL SFKLLLFAFGKGPLCS A SSSL RFLAV SLPI+IP+H+P  NRSDP P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGV 180
           +LPLSF+AKLG LILTFAAIYFKN  + N  LIFFCL+IYLLLEILIGGA A+ +A LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 DLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTAT 240
           +LLP+FDEPYLSDSLQDFWGRRWNL++SRILR AV+DPCRKL   +VG+KPA +IAV AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLT 300
           FAVSGVMHELIYFYMGRLAPTWEV+ FFL+HGVCLAAEM +KSAVG RFR P +VS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 IGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQLITPLKFISFQLK 356
           +GFVM TSFWLFFPQ LRFKADVRM EE+AALGAFVK+VTA+LI PL FISFQ+K
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGPL-FISFQVK 354

BLAST of HG10019395.1 vs. ExPASy TrEMBL
Match: A0A6J1F144 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441426 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 8.3e-154
Identity = 282/355 (79.44%), Postives = 312/355 (87.89%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           MAAEI  F+QTLFL++SSLC CFSIRNSIPNGF KF  +LPFFFLFLYIPLQFQTIHL+G
Sbjct: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
           ++GFFIGWL SFKLLLFAFGKGPLC  A SSSL RFLAV  LPI+IP+H+   NRSDP P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGV 180
           +LPLSF+AKLG LILTFAAIYFKN  + N  LIFFCL+IYLLLEILIGGA AL +A LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 DLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTAT 240
           +LLP+FDEPYLSDSLQDFWGRRWNL++SRILR AV+DPCRKL   +VG+KPAA+IAV AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240

Query: 241 FAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLT 300
           FAVSGVMHELIYFYMGRL PTWEV+ FFL+HGVCLAAEM +KSAVGGRFR P +VS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300

Query: 301 IGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQLITPLKFISFQLK 356
           IGFVM TSFWLFFPQ LRFKADVRM EE+AALGAFVKSVTA+LI PL FISFQ+K
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL-FISFQVK 354

BLAST of HG10019395.1 vs. ExPASy TrEMBL
Match: A0A0A0LES4 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684670 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.1e-149
Identity = 281/349 (80.52%), Postives = 301/349 (86.25%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           MAAEIRIFLQTL LVSSSL FCFSIRNSIPNGF +F LILPFF LFLYIPLQFQTIHLQG
Sbjct: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
           SIGFFIGWLASFKLLLFAFGKGPLCS AASSSL RFLA+GSLPIEIP      NRS P P
Sbjct: 61  SIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQD---TNRSHPNP 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGV 180
           +LP ++  KL LLILT  AIYFKN+LH   YLI FC VIY LLEILIGGAAA VKA LGV
Sbjct: 121 ILPPTYFIKLLLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGV 180

Query: 181 DLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTAT 240
           +LLPYFDEPYLS SLQDFWG+RWNL+TSRILRLAVYDPCRKLTVGIVG+K +AM+A+TAT
Sbjct: 181 ELLPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMVALTAT 240

Query: 241 FAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLT 300
           FAVSG+MHELIYFYMGRLAPTWEVTCFFLLHGV L+AEM +KSAV GR ++P I+S   T
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFT 300

Query: 301 IGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQLITPLKF 350
             FVMTTS WLFFPQF R K DVRM EEHAALGAFVK+VT QLITP KF
Sbjct: 301 WWFVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKF 346

BLAST of HG10019395.1 vs. ExPASy TrEMBL
Match: A0A5D3BDP7 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold939G00060 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.6e-149
Identity = 278/355 (78.31%), Postives = 306/355 (86.20%), Query Frame = 0

Query: 2   AAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQGS 61
           AAEIRIFLQTLFLVSSSL FCFSIRNSIPNGF KF  +LPFFFLFLY+PLQFQT+HLQG 
Sbjct: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62

Query: 62  IGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGPV 121
            GFFIGWLASFKLLLFAFGKGPL S AASSSL RFLA+GSLPIEIP      N SDP P+
Sbjct: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQD---TNPSDPNPI 122

Query: 122 LPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGVD 181
           LP ++  KLGLLILTF A+YF+ +LH N YLI FC +IY LLEILIGGAAA VKA LGV+
Sbjct: 123 LPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVE 182

Query: 182 LLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTATF 241
           LLPYFDEPYLS SLQDFWG+RWNLM SRILRLAVYDPCRKLT+GIVG+KP+A++A+TATF
Sbjct: 183 LLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATF 242

Query: 242 AVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLTI 301
           AVSG+MHELIYFYMGR+APTWEVTCFFLLHGV L+AEM +KSAVGGRFR+P IVS   T 
Sbjct: 243 AVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTW 302

Query: 302 GFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQLITPLKF-ISFQLK 356
            FVMTTS WLFFPQF R K +VRMFEEHAAL AFVK+VT QLITP KF + F +K
Sbjct: 303 WFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 354

BLAST of HG10019395.1 vs. ExPASy TrEMBL
Match: A0A1S3CFI3 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC103500342 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.6e-149
Identity = 278/355 (78.31%), Postives = 306/355 (86.20%), Query Frame = 0

Query: 2   AAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQGS 61
           AAEIRIFLQTLFLVSSSL FCFSIRNSIPNGF KF  +LPFFFLFLY+PLQFQT+HLQG 
Sbjct: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62

Query: 62  IGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGPV 121
            GFFIGWLASFKLLLFAFGKGPL S AASSSL RFLA+GSLPIEIP      N SDP P+
Sbjct: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQD---TNPSDPNPI 122

Query: 122 LPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGVD 181
           LP ++  KLGLLILTF A+YF+ +LH N YLI FC +IY LLEILIGGAAA VKA LGV+
Sbjct: 123 LPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVE 182

Query: 182 LLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTATF 241
           LLPYFDEPYLS SLQDFWG+RWNLM SRILRLAVYDPCRKLT+GIVG+KP+A++A+TATF
Sbjct: 183 LLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATF 242

Query: 242 AVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLTI 301
           AVSG+MHELIYFYMGR+APTWEVTCFFLLHGV L+AEM +KSAVGGRFR+P IVS   T 
Sbjct: 243 AVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTW 302

Query: 302 GFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQLITPLKF-ISFQLK 356
            FVMTTS WLFFPQF R K +VRMFEEHAAL AFVK+VT QLITP KF + F +K
Sbjct: 303 WFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 354

BLAST of HG10019395.1 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 258.8 bits (660), Expect = 6.2e-69
Identity = 145/338 (42.90%), Postives = 200/338 (59.17%), Query Frame = 0

Query: 8   FLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQGSIGFFIG 67
           F++   LV  SLC+ F I   +P G  +  L  P F +F  +P    ++HL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIP-DHEPAKNRSDPGPVL-PLS 127
           WLA+FKLLLFA G+GPL S     SL  FLAV  LPI+I    +P K  S  G    PL 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 FSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGVDLLPY 187
           ++ K   ++L   A  +   L +   L  + + IY  LEI++   AA V+A   ++L P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 188 FDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTATFAVSG 247
           F++PYL+ SLQDFWGRRWNLM + ILR  VY+P  +L   ++G   + ++A   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 248 VMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVSAPLTIGFVM 307
           +MHELI+FYMGRL P W++  FFL++G C   E+ IK  + GR+R P  +S  LT+ FVM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 TTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVTAQL 344
            T+ WLF P+F R     +  +E+AA+GAF   V  +L
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKL 340

BLAST of HG10019395.1 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 219.2 bits (557), Expect = 5.4e-57
Identity = 134/338 (39.64%), Postives = 189/338 (55.92%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           M  E+   ++       S+ +C+     I +G F+   +LP   LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
           S  FF+ WLA+FKL+LF+F +GPL      S+L RF+     PI++  +   +N+     
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKPQNQ----- 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALGV 180
           +    F+ KL    +      +K H+     L+ + L IYL  EIL+     L+  +L  
Sbjct: 121 MPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWC 180

Query: 181 DLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTAT 240
           DL P+F+EPYLS SLQDFWGRRWNLM   ILR AVY P R++    +    A  + V A+
Sbjct: 181 DLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFAS 240

Query: 241 FAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIK-SAVGGRFRLPGIVSAPL 300
           F VSGV+HELI+FY  R +PT EVT FF+LHGVC AAE   K + +  R+++  +VS  L
Sbjct: 241 FLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLL 300

Query: 301 TIGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVK 338
           T+GFV+ T  WLFFP   R     R+ +E      FVK
Sbjct: 301 TVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

BLAST of HG10019395.1 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 211.8 bits (538), Expect = 8.7e-55
Identity = 129/317 (40.69%), Postives = 182/317 (57.41%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           M  E++ F++       S+ +C+ +   I  G F+   +LP   LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
            + FF+ WLA+FKL+LF+F +GPL  +    +L RF+ +   PI     +P +N +    
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPI-----KPQQNPNIQNY 120

Query: 121 VLPL-SFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALG 180
            +P+  F+ K+ + ++      +K +L     L+F  L I+L LEI+     ALV   LG
Sbjct: 121 KIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLG 180

Query: 181 VDLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTA 240
            DL P  +EPYL+ SLQDFWGRRWNLM   ILR AVY P R++    V    A  + V A
Sbjct: 181 CDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFA 240

Query: 241 TFAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGR--FRLPGIVSA 300
            F VSG +HE+++FY+ R  PT EVT FFLLHGVC  AE+ +K +   R  +R+   VS 
Sbjct: 241 AFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSR 300

Query: 301 PLTIGFVMTTSFWLFFP 315
            LT+GFV+ TS W FFP
Sbjct: 301 LLTVGFVVVTSGWFFFP 310

BLAST of HG10019395.1 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.4e-52
Identity = 126/332 (37.95%), Postives = 186/332 (56.02%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           M  E+R  ++       S+ +C+ I + I  G  +   +LP F +FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
             GFF  WLA+FKL LFAF + PL  +   S+L RF      PI+I +  P+ NR    P
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKI-NKNPSSNRIHNKP 120

Query: 121 VLPLSFSAKLGLLILTFAAIYFKNH----LHQNAYLIFFCLVIYLLLEILIGGAAALVKA 180
           +     + K  LLI +F    ++N+    L + A+L  F + +YL  E+++    AL+  
Sbjct: 121 MSKWVLAFK--LLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSM 180

Query: 181 ALGVDLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIA 240
            LG ++ P F++PYL+ SLQ+FW RRWNLM   +LR AV+ P ++    ++G   A    
Sbjct: 181 LLGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAG 240

Query: 241 VTATFAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGIKSAVGGRFRLPGIVS 300
           + ATF VSG+MHELIYFY+ R +PTWEVTCFFLLHGV    E+ +K           +  
Sbjct: 241 MLATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKRMRWLPTPRRAVSG 300

Query: 301 APLTIGFVMTTSFWLFFPQFLRFKADVRMFEE 329
             +T+ F++ T+ WLF+PQ LR     R+  E
Sbjct: 301 LAITV-FLLVTAGWLFYPQMLRNDVHKRVISE 326

BLAST of HG10019395.1 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 199.5 bits (506), Expect = 4.5e-51
Identity = 129/340 (37.94%), Postives = 187/340 (55.00%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLCFCFSIRNSIPNGFFKFFLILPFFFLFLYIPLQFQTIHLQG 60
           M  E++  ++       S+ +C+ I   I +G  +F  + P   LFL +PL F ++HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSTAASSSLCRFLAVGSLPIEIPDHEPAKNRSDPGP 120
              FF+ WLA+FKL+LF+F KGPL      ++  RF      PI++  +  ++N      
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPL--IPIPTNFPRFFCFTCFPIKVQQNPKSQNH----- 120

Query: 121 VLP-LSFSAKLGLLILTFAAIYFKNHLHQNAYLIFFCLVIYLLLEILIGGAAALVKAALG 180
            LP L F+ KL +  +      ++ +L     L  + + +YL +EI++     +V  +LG
Sbjct: 121 -LPKLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLG 180

Query: 181 VDLLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLAVYDPCRKLTVGIVGRKPAAMIAVTA 240
            DL P  ++PYL+ SLQDFWGRRWNLM   ILR AVY P R+++   +    A    + A
Sbjct: 181 CDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILA 240

Query: 241 TFAVSGVMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMGI--KSAVGGRFRLPGIVSA 300
            F VSG++HEL++FY+ R  PT EVT FF+LHGVC A E+ +  K+ V  R+RL   VS 
Sbjct: 241 AFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSR 300

Query: 301 PLTIGFVMTTSFWLFFPQFLRFKADVRMFEEHAALGAFVK 338
            LT+GFV  T  WLF PQ  R     R   E   L  F+K
Sbjct: 301 VLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332