HG10019364.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10019364.1 vs. NCBI nr
Match: XP_038905655.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 904.0 bits (2335), Expect = 5.4e-259
Identity = 437/488 (89.55%), Postives = 455/488 (93.24%), Query Frame = 0

Query: 1   MYFIGSFSSFSFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQPPSPSPS 60
           MYF G FSS +FCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHH+H FTISQPPSP P+
Sbjct: 1   MYFRG-FSSLAFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHNH-FTISQPPSPPPT 60

Query: 61  PSSEPTGPPDDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDND 120
                + P DDDNGGS+SINST IFDVR+FGAIGDGT DDT AFKMAWDTACQ D+D   
Sbjct: 61  -----SPPDDDDNGGSSSINSTGIFDVRNFGAIGDGTADDTMAFKMAWDTACQRDDD--- 120

Query: 121 IDNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVF 180
            D++YAVIL+PY FSFMIQSTIFTGPCKNG+ FQV GT+MAPDGPDAWPKSYSA QWLVF
Sbjct: 121 -DDNYAVILIPYGFSFMIQSTIFTGPCKNGVEFQVGGTLMAPDGPDAWPKSYSAHQWLVF 180

Query: 181 YRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRVEGV 240
           YRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKG TM+  CDSPIAIRFFMSS L VEG+
Sbjct: 181 YRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGRTMSEPCDSPIAIRFFMSSNLTVEGL 240

Query: 241 RIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCIS 300
           RIKDSPQFHFRFDNCKDV IDGIHITAPALSPNTDGIHIENTN VQIFNS VANGDDCIS
Sbjct: 241 RIKDSPQFHFRFDNCKDVIIDGIHITAPALSPNTDGIHIENTNGVQIFNSEVANGDDCIS 300

Query: 301 IGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG 360
           IGSGSYNVDI+NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG
Sbjct: 301 IGSGSYNVDIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG 360

Query: 361 YGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIRSPP 420
           YGAVRGVSFNNINM+NVRNPIIIDQFYCLNKGCLNQTSAV+VSDITYVDIKGTYDIRSPP
Sbjct: 361 YGAVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDITYVDIKGTYDIRSPP 420

Query: 421 MHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESSSRSI 480
           MHFGCSDSMPCMNLTLANIELFPSKGE+LLDPFCWNAYGNLQTLTIPPVACLLESSSRS 
Sbjct: 421 MHFGCSDSMPCMNLTLANIELFPSKGEVLLDPFCWNAYGNLQTLTIPPVACLLESSSRSF 477

Query: 481 LESEKDYC 489
           LESEKDYC
Sbjct: 481 LESEKDYC 477

BLAST of HG10019364.1 vs. NCBI nr
Match: TYK20501.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 899.8 bits (2324), Expect = 1.0e-257
Identity = 436/499 (87.37%), Postives = 459/499 (91.98%), Query Frame = 0

Query: 1   MYFIGS-FSSFSFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQP----- 60
           MY  G   SSF+FCITFIIFFFCLLFPV+GRWHNHAKKHKQHSHHH H FTISQP     
Sbjct: 1   MYNFGRLISSFTFCITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYH-FTISQPPSPFP 60

Query: 61  -----PSPSPSPSSEPTGPPDDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWD 120
                PSPSPSPSSEP  PP+DD G ++SINST IFDVRHFGAIGDGT DDTAAFKMAWD
Sbjct: 61  SPSPSPSPSPSPSSEPQSPPEDDGGPASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWD 120

Query: 121 TACQWDNDDNDIDNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWP 180
           TACQ ++DD   DN+Y VILVPY FSFMIQSTIFTGPC+NGIVFQVDGT+MAPDGPDAWP
Sbjct: 121 TACQRNDDD---DNNYGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWP 180

Query: 181 KSYSARQWLVFYRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRF 240
           K YSA QWLVFYRVYNMSL+GNGLIDGRGQKWWDLPCKPH   KG TMTG CDSPIAIRF
Sbjct: 181 KGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWWDLPCKPH---KGKTMTGPCDSPIAIRF 240

Query: 241 FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFN 300
           FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYID +HIT+PALSPNTDGIHIENTN VQIFN
Sbjct: 241 FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFN 300

Query: 301 SVVANGDDCISIGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD 360
           S+VANGDDCISIGSGSYNV+I NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD
Sbjct: 301 SIVANGDDCISIGSGSYNVEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD 360

Query: 361 NGVRIKTWQGGYGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVD 420
           NGVRIKTWQGGYGAVRGVSFNNINM+NVRNPIIIDQFYCLNKGCLNQTSAV+VSDI+Y+D
Sbjct: 361 NGVRIKTWQGGYGAVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYID 420

Query: 421 IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPV 480
           IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKG+ILLDPFCWNAYGNLQTLTIPP+
Sbjct: 421 IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPI 480

Query: 481 ACLLESSSRSILESEKDYC 489
           +CLLE+SSRS LESEKDYC
Sbjct: 481 SCLLETSSRSFLESEKDYC 492

BLAST of HG10019364.1 vs. NCBI nr
Match: XP_031737994.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN58717.2 hypothetical protein Csa_000831 [Cucumis sativus])

HSP 1 Score: 896.3 bits (2315), Expect = 1.1e-256
Identity = 431/491 (87.78%), Postives = 460/491 (93.69%), Query Frame = 0

Query: 1   MYFIGS-FSSFSFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQPPSPSP 60
           MY  G   SSF+F +TFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDH FTISQP  PSP
Sbjct: 1   MYNFGRLISSFAFSMTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDH-FTISQP--PSP 60

Query: 61  SPSSEPTGPPDDDNGG--STSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDND 120
           SPSSEP G P +DNGG  S+SINSTSIFDVRHFGAIGDGT DDTAAFKMAWDTACQ ++D
Sbjct: 61  SPSSEPAGSPPEDNGGPSSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDD 120

Query: 121 DNDIDNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQW 180
           DN  ++DY VILVPY FSFMIQSTIFTGPCKNG+VFQVDGT+MAPDGPDAWPK YSA QW
Sbjct: 121 DN--NDDYGVILVPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQW 180

Query: 181 LVFYRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRV 240
           LVFYRVYNMSL+GNGLIDGRGQKWWDLPCKPH   KG TMTG CDSPIAIRFFMSS+LRV
Sbjct: 181 LVFYRVYNMSLQGNGLIDGRGQKWWDLPCKPH---KGKTMTGPCDSPIAIRFFMSSELRV 240

Query: 241 EGVRIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDD 300
           EGVRIKDSPQFHFRFDNCKDVYID +HIT+PALSPNTDGIHIENTN VQIFNS+VANGDD
Sbjct: 241 EGVRIKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDD 300

Query: 301 CISIGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTW 360
           C+SIGSGS+NV+I+NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTW
Sbjct: 301 CVSIGSGSFNVEIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTW 360

Query: 361 QGGYGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIR 420
           QGG+G VRGVSFNNINM+NVRNPIIIDQFYCLNKGCLNQTSAV+VSDI+Y+DIKGTYDIR
Sbjct: 361 QGGFGTVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIR 420

Query: 421 SPPMHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESSS 480
           SPPMHFGCSDSMPCMNLTLANIELFPS+G+ILLDPFCWNAYGNLQTLTIPP++CLLE+SS
Sbjct: 421 SPPMHFGCSDSMPCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSS 480

Query: 481 RSILESEKDYC 489
           RS LESEKDYC
Sbjct: 481 RSFLESEKDYC 483

BLAST of HG10019364.1 vs. NCBI nr
Match: KAA0038107.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 873.2 bits (2255), Expect = 1.0e-249
Identity = 426/499 (85.37%), Postives = 449/499 (89.98%), Query Frame = 0

Query: 1   MYFIGS-FSSFSFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQP----- 60
           MY  G   SSF+FCITFIIFFFCLLFPV+GRWHNHAKKHKQHSHHH H FTISQP     
Sbjct: 1   MYNFGRLISSFTFCITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYH-FTISQPPSPFP 60

Query: 61  -----PSPSPSPSSEPTGPPDDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWD 120
                PSPSPSPSSEP  PP+DD G ++SINST IFDVRHFGAIGDGT DDTAAFKMAWD
Sbjct: 61  SPSPSPSPSPSPSSEPQSPPEDDGGPASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWD 120

Query: 121 TACQWDNDDNDIDNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWP 180
           TACQ ++DD   DN+Y VILVPY FSFMIQSTIFTGPC+NGIVFQVDGT+MAPDGPDAWP
Sbjct: 121 TACQRNDDD---DNNYGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWP 180

Query: 181 KSYSARQWLVFYRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRF 240
           K YSA QWLVFYRVYNMSL+GNGLIDGRGQKWWDLPCKPHK               AIRF
Sbjct: 181 KGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHKSKSVSFFWQ------AIRF 240

Query: 241 FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFN 300
           FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYID +HIT+PALSPNTDGIHIENTN VQIFN
Sbjct: 241 FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFN 300

Query: 301 SVVANGDDCISIGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD 360
           S+VANGDDCISIGSGSYNV+I NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD
Sbjct: 301 SIVANGDDCISIGSGSYNVEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD 360

Query: 361 NGVRIKTWQGGYGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVD 420
           NGVRIKTWQGGYGAVRGVSFNNINM+NVRNPIIIDQFYCLNKGCLNQTSAV+VSDI+Y+D
Sbjct: 361 NGVRIKTWQGGYGAVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYID 420

Query: 421 IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPV 480
           IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKG+ILLDPFCWNAYGNLQTLTIPP+
Sbjct: 421 IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPI 480

Query: 481 ACLLESSSRSILESEKDYC 489
           +CLLE+SSRS LESEKDYC
Sbjct: 481 SCLLETSSRSFLESEKDYC 489

BLAST of HG10019364.1 vs. NCBI nr
Match: XP_022935453.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 846.3 bits (2185), Expect = 1.3e-241
Identity = 410/488 (84.02%), Postives = 438/488 (89.75%), Query Frame = 0

Query: 7   FSSFSFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQPPSPSP------S 66
           F SF+ CIT IIFF    FPVHGRWHNHAK HK HSHHH H FTISQPPSPSP      S
Sbjct: 5   FCSFALCITLIIFF----FPVHGRWHNHAKNHK-HSHHHPH-FTISQPPSPSPSPSPPLS 64

Query: 67  PSSEPTGPPDDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDND 126
           PS EP  PPDD++GGS S N+T  FDVRH+GAIGDGTTDDT AFKMAWDTACQ DND   
Sbjct: 65  PSPEPANPPDDNDGGS-SFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDND--- 124

Query: 127 IDNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVF 186
              DYAVILVPY FSFMIQSTIFTGPCK+GIVFQVDGT +APDGPDAWPKSYSA QWLVF
Sbjct: 125 ---DYAVILVPYGFSFMIQSTIFTGPCKHGIVFQVDGTFIAPDGPDAWPKSYSAHQWLVF 184

Query: 187 YRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRVEGV 246
           YRV NMSLRGNG+IDGRGQKWWDLPCKPH+G KG T  G CDSPIAIRFFMSS L VEG+
Sbjct: 185 YRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGL 244

Query: 247 RIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCIS 306
           RIKDSPQFHFRFDNC+DVYID + ITAPA SPNTDGIHIENTN VQI NSVVANGDDCIS
Sbjct: 245 RIKDSPQFHFRFDNCRDVYIDSLTITAPAWSPNTDGIHIENTNGVQILNSVVANGDDCIS 304

Query: 307 IGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG 366
           IGSGSYNVDI+NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG
Sbjct: 305 IGSGSYNVDIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG 364

Query: 367 YGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIRSPP 426
           +GAVRGVSFNNI+M+NVRNPIIIDQFYCLNKGCLNQTSAV+VSDI+Y+DIKG+YDIRSPP
Sbjct: 365 FGAVRGVSFNNIHMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGSYDIRSPP 424

Query: 427 MHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESSSRSI 486
           MHFGCSDSMPC+NLTLANIELFPSKG+I LDPFCWNAYG+LQTLTIPP+ CLLE+SSRSI
Sbjct: 425 MHFGCSDSMPCVNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASSRSI 479

Query: 487 LESEKDYC 489
           LE+++DYC
Sbjct: 485 LETQRDYC 479

BLAST of HG10019364.1 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.3e-146
Identity = 247/466 (53.00%), Postives = 326/466 (69.96%), Query Frame = 0

Query: 17  IIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQPPSPSP------SPSSEPTGPPD 76
           I+  + +   + G + N A+  + H H   H  T   PP P P       P   P+ P  
Sbjct: 7   ILVIYIISTVLFGHFTNVARA-RYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYP 66

Query: 77  DDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDNDIDNDYAVILV 136
             +      +S  +FDV  FGA+GDG+ DDTAAF+ AW  AC  ++          V+L 
Sbjct: 67  SPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVES---------GVVLA 126

Query: 137 PYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVFYRVYNMSLRG 196
           P    F I STIF+GPCK G+VFQ+DG +M PDGP+ WP+  +  QWLVFYR+   +  G
Sbjct: 127 PEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSG 186

Query: 197 NGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRVEGVRIKDSPQFHF 256
            G ++G GQKWWDLPCKPH+G  G + +G C SP  IRFFMS+ + V+G+RI++SPQFH 
Sbjct: 187 KGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHM 246

Query: 257 RFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCISIGSGSYNVDI 316
           +FD C+ V I+ I I++P LSPNTDGIH+ NT +V I+NSVV+NGDDCISIG+G  +VDI
Sbjct: 247 KFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDI 306

Query: 317 KNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFN 376
           + +TCGP HGISIGSLG HNS+ACVSNITVR+++I+ SDNG+R+KTWQGG G+V  + F 
Sbjct: 307 QGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFE 366

Query: 377 NINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIRSPPMHFGCSDSMP 436
           NI MENV N II+DQ+YC +K C N+TSAV V D+ Y +IKGTYD+RSPP+HF CSD++ 
Sbjct: 367 NIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVA 426

Query: 437 CMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESS 477
           C N+T++ +EL P +GE++ DPFCWNAYG  +TLTIPP+ CLL+ S
Sbjct: 427 CTNITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGS 462

BLAST of HG10019364.1 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 2.7e-91
Identity = 173/404 (42.82%), Postives = 237/404 (58.66%), Query Frame = 0

Query: 76  STSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDNDIDNDYAVILVPYDFS 135
           ST   S  +  V +FGA G+G TDDT AF  AW TAC               ILVP +++
Sbjct: 44  STRPRSERLLHVGNFGAKGNGVTDDTKAFADAWKTACS--------SKVKTRILVPENYT 103

Query: 136 FMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVFYRVYNMSLRGNGLID 195
            +++    +GPCK  +  Q+ GT++AP+ PD W +  + R+WL F+ +  +++ G G ++
Sbjct: 104 CLLRPIDLSGPCKARLTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVN 163

Query: 196 GRGQKWWDLPCKPHKGVKGGTMTGAC-DSPIAIRFFMSSKLRVEGVRIKDSPQFHFRFDN 255
           G GQ+WW+  CK +        +  C  +P A+ F     +RVE + + DS Q H    +
Sbjct: 164 GMGQEWWERSCKHNH-------SNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTS 223

Query: 256 CKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCISIGSGSYNVDIKNIT 315
           C+ V I G+ + APA SPNTDGIHI  +  + I N+ V+ GDDCISI   S  + I NI 
Sbjct: 224 CRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNII 283

Query: 316 CGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFNNINM 375
           CGPGHGISIGSLG   S   V +ITV  +II  + NGVRIKTWQGG G V  + F NI M
Sbjct: 284 CGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKM 343

Query: 376 ENVRNPIIIDQFYC-LNKGCLNQTSAVLVSDITYVDIKGTYDIRSPPMHFGCSDSMPCMN 435
            NV NPIIIDQ+YC   K C NQTSA+ + +I++V ++GT       +   CSDS PC N
Sbjct: 344 NNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRN 403

Query: 436 LTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESSS 478
           + L +I+L PS G+   + FCW AYG+      PP  CL + +S
Sbjct: 404 ILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYPP-PCLSDDTS 429

BLAST of HG10019364.1 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 4.5e-83
Identity = 168/410 (40.98%), Postives = 239/410 (58.29%), Query Frame = 0

Query: 80  NSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDNDIDNDYAVILVPYDFSFMIQ 139
           ++ +IF+V  +GA+GDG  D T AF  AW  AC+            A++LVP +  F++ 
Sbjct: 54  DAINIFNVEKYGAVGDGKHDCTEAFSTAWQAACK---------KPSAMLLVPGNKKFVVN 113

Query: 140 STIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVFYRVYNMSLRGNGLIDGRGQ 199
           +  F GPC+    F+VDG + A   P +W  +   R WL F ++   +L G G+IDG+G+
Sbjct: 114 NLFFNGPCQPHFTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGK 173

Query: 200 KWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRVEGVRIKDSPQFHFRFDNCKDVY 259
           +WW   C   K V G  +    D P AI+F  S+ L ++G+++ +SP+FH  F NC+ V 
Sbjct: 174 QWWAGQC---KWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVK 233

Query: 260 IDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCISIGSGSYNVDIKNITCGPGH 319
           I GI ITAP  SPNTDGI I  +    +  + +  GDDC++IG+GS N+ I+++ CGPGH
Sbjct: 234 IIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGH 293

Query: 320 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFNNINMENVRN 379
           GISIGSLG  NSRA VS + V  +    + NG+RIKTWQGG G    + + N+ M N  N
Sbjct: 294 GISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSEN 353

Query: 380 PIIIDQFYCLN-KGCLNQTSAVLVSDITYVDIKGTYDIRSPPMHFGCSDSMPCMNLTLAN 439
           PI+I+QFYC +   C NQ SAV + D+TY +I+GT    +  +   CSDSMPC ++ L++
Sbjct: 354 PILINQFYCTSASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSD 413

Query: 440 IELFPSKGEI---LLDPFCWNAYGNLQTLTIPPVACLLESSSRSILESEK 486
           I L  + G+I   L D    NA G      IP    L  S+ R   +S K
Sbjct: 414 ISLKLTSGKIASCLND----NANGYFSGHVIPACKNLSPSAKRKESKSHK 443

BLAST of HG10019364.1 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.6e-80
Identity = 153/369 (41.46%), Postives = 221/369 (59.89%), Query Frame = 0

Query: 80  NSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDNDIDNDYAVILVPYDFSFMIQ 139
           ++ ++F+V  +GA+GDG  D T AF   W+ AC+            AV+LVP +  F + 
Sbjct: 54  DAATVFNVEQYGAVGDGKHDSTEAFATTWNAACK---------KASAVLLVPANKKFFVN 113

Query: 140 STIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVFYRVYNMSLRGNGLIDGRGQ 199
           + +F GPC+  + F+VDGT++A   P  W  S   + WL F ++ + +L G G+IDG+GQ
Sbjct: 114 NLVFRGPCQPHLSFKVDGTIVAQPDPARWKNS---KIWLQFAQLTDFNLMGTGVIDGQGQ 173

Query: 200 KWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRVEGVRIKDSPQFHFRFDNCKDVY 259
           +WW   CK    V G T+    + P AI+   S  + V+ + + +SP+FH  F  C+ V 
Sbjct: 174 QWWAGQCKV---VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVK 233

Query: 260 IDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCISIGSGSYNVDIKNITCGPGH 319
           I G+ I AP  SPNTDGI I  +    I   V+  GDDCI+IG+GS N+ IK++ CGPGH
Sbjct: 234 IQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGH 293

Query: 320 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFNNINMENVRN 379
           GISIGSLG  NSRA VS++ V  +    + NG+RIKTWQGG G    +++ N+ M N  N
Sbjct: 294 GISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSEN 353

Query: 380 PIIIDQFYCLN-KGCLNQTSAVLVSDITYVDIKGTYDIRSPPMHFGCSDSMPCMNLTLAN 439
           PI+I+QFYC +   C NQ SAV +  +TY +I GT    +  +   CSDS+PC  + L+N
Sbjct: 354 PILINQFYCTSASACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSN 406

Query: 440 IELFPSKGE 448
           + L  + G+
Sbjct: 414 VSLKLTSGK 406

BLAST of HG10019364.1 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 6.1e-80
Identity = 161/376 (42.82%), Postives = 218/376 (57.98%), Query Frame = 0

Query: 74  GGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDNDIDNDYAVILVPYD 133
           GG  +   T I  V  FGA GDG TDDT AF+ AW  AC          +  +V++VP +
Sbjct: 75  GGPETSPDTDI-SVDDFGARGDG-TDDTKAFEKAWKDAC----------SSGSVLIVPEN 134

Query: 134 FSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVFYRVYNMSLRGNGL 193
            +++++   F+GPCK+ +  ++ GT+ A      W   ++ ++W+ F  + N++L G G 
Sbjct: 135 KNYLLKQITFSGPCKSDLRVKIRGTIEASSDQSDW-VGHNRKRWIEFEDISNLTLEGGGT 194

Query: 194 IDGRGQKWWDLPCKPHKGVKGGTMTGACDS-PIAIRFFMSSKLRVEGVRIKDSPQFHFRF 253
            +G G+ WWD  CK  K +        C S P A+ F     L V  + IKDS + H  F
Sbjct: 195 SNGNGETWWDSSCKRKKSL-------PCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSF 254

Query: 254 DNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCISIGSGSYNVDIKN 313
           D C+DV    + +TAP  SPNTDGIHI  T  + + NSV+  GDDCISI SGS  V   N
Sbjct: 255 DKCQDVIASNLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATN 314

Query: 314 ITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFNNI 373
           ITCGPGHGISIGSLG+ NS A VS + V    +  + NG+RIKTWQGG G+ + + F NI
Sbjct: 315 ITCGPGHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNI 374

Query: 374 NMENVRNPIIIDQFYCLNKG-CLNQTSAVLVSDITYVDIKGTYDIRSPPMHFGCSDSMPC 433
            M NV NPIIIDQ+YC +K  C  Q SAV VS++ Y++I+GT       + F CS S PC
Sbjct: 375 VMHNVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGT-SASEVAVKFDCSKSSPC 429

Query: 434 MNLTLANIELFPSKGE 448
               + NI L  + G+
Sbjct: 435 QGYIVGNINLVGNGGK 429

BLAST of HG10019364.1 vs. ExPASy TrEMBL
Match: A0A5D3DA93 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00750 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 5.0e-258
Identity = 436/499 (87.37%), Postives = 459/499 (91.98%), Query Frame = 0

Query: 1   MYFIGS-FSSFSFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQP----- 60
           MY  G   SSF+FCITFIIFFFCLLFPV+GRWHNHAKKHKQHSHHH H FTISQP     
Sbjct: 1   MYNFGRLISSFTFCITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYH-FTISQPPSPFP 60

Query: 61  -----PSPSPSPSSEPTGPPDDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWD 120
                PSPSPSPSSEP  PP+DD G ++SINST IFDVRHFGAIGDGT DDTAAFKMAWD
Sbjct: 61  SPSPSPSPSPSPSSEPQSPPEDDGGPASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWD 120

Query: 121 TACQWDNDDNDIDNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWP 180
           TACQ ++DD   DN+Y VILVPY FSFMIQSTIFTGPC+NGIVFQVDGT+MAPDGPDAWP
Sbjct: 121 TACQRNDDD---DNNYGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWP 180

Query: 181 KSYSARQWLVFYRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRF 240
           K YSA QWLVFYRVYNMSL+GNGLIDGRGQKWWDLPCKPH   KG TMTG CDSPIAIRF
Sbjct: 181 KGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWWDLPCKPH---KGKTMTGPCDSPIAIRF 240

Query: 241 FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFN 300
           FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYID +HIT+PALSPNTDGIHIENTN VQIFN
Sbjct: 241 FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFN 300

Query: 301 SVVANGDDCISIGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD 360
           S+VANGDDCISIGSGSYNV+I NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD
Sbjct: 301 SIVANGDDCISIGSGSYNVEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD 360

Query: 361 NGVRIKTWQGGYGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVD 420
           NGVRIKTWQGGYGAVRGVSFNNINM+NVRNPIIIDQFYCLNKGCLNQTSAV+VSDI+Y+D
Sbjct: 361 NGVRIKTWQGGYGAVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYID 420

Query: 421 IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPV 480
           IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKG+ILLDPFCWNAYGNLQTLTIPP+
Sbjct: 421 IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPI 480

Query: 481 ACLLESSSRSILESEKDYC 489
           +CLLE+SSRS LESEKDYC
Sbjct: 481 SCLLETSSRSFLESEKDYC 492

BLAST of HG10019364.1 vs. ExPASy TrEMBL
Match: A0A0A0LEQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.8e-255
Identity = 424/477 (88.89%), Postives = 452/477 (94.76%), Query Frame = 0

Query: 14  ITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQPPSPSPSPSSEPTGPPDDDN 73
           +TFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDH FTISQP  PSPSPSSEP G P +DN
Sbjct: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDH-FTISQP--PSPSPSSEPAGSPPEDN 60

Query: 74  GG--STSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDNDIDNDYAVILVP 133
           GG  S+SINSTSIFDVRHFGAIGDGT DDTAAFKMAWDTACQ ++DDN  ++DY VILVP
Sbjct: 61  GGPSSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDN--NDDYGVILVP 120

Query: 134 YDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVFYRVYNMSLRGN 193
           Y FSFMIQSTIFTGPCKNG+VFQVDGT+MAPDGPDAWPK YSA QWLVFYRVYNMSL+GN
Sbjct: 121 YGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGN 180

Query: 194 GLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRVEGVRIKDSPQFHFR 253
           GLIDGRGQKWWDLPCKPH   KG TMTG CDSPIAIRFFMSS+LRVEGVRIKDSPQFHFR
Sbjct: 181 GLIDGRGQKWWDLPCKPH---KGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFR 240

Query: 254 FDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCISIGSGSYNVDIK 313
           FDNCKDVYID +HIT+PALSPNTDGIHIENTN VQIFNS+VANGDDC+SIGSGS+NV+I+
Sbjct: 241 FDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIR 300

Query: 314 NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFNN 373
           NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G VRGVSFNN
Sbjct: 301 NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNN 360

Query: 374 INMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIRSPPMHFGCSDSMPC 433
           INM+NVRNPIIIDQFYCLNKGCLNQTSAV+VSDI+Y+DIKGTYDIRSPPMHFGCSDSMPC
Sbjct: 361 INMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPC 420

Query: 434 MNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESSSRSILESEKDYC 489
           MNLTLANIELFPS+G+ILLDPFCWNAYGNLQTLTIPP++CLLE+SSRS LESEKDYC
Sbjct: 421 MNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC 469

BLAST of HG10019364.1 vs. ExPASy TrEMBL
Match: A0A5A7T924 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003270 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 5.0e-250
Identity = 426/499 (85.37%), Postives = 449/499 (89.98%), Query Frame = 0

Query: 1   MYFIGS-FSSFSFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQP----- 60
           MY  G   SSF+FCITFIIFFFCLLFPV+GRWHNHAKKHKQHSHHH H FTISQP     
Sbjct: 1   MYNFGRLISSFTFCITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYH-FTISQPPSPFP 60

Query: 61  -----PSPSPSPSSEPTGPPDDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWD 120
                PSPSPSPSSEP  PP+DD G ++SINST IFDVRHFGAIGDGT DDTAAFKMAWD
Sbjct: 61  SPSPSPSPSPSPSSEPQSPPEDDGGPASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWD 120

Query: 121 TACQWDNDDNDIDNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWP 180
           TACQ ++DD   DN+Y VILVPY FSFMIQSTIFTGPC+NGIVFQVDGT+MAPDGPDAWP
Sbjct: 121 TACQRNDDD---DNNYGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWP 180

Query: 181 KSYSARQWLVFYRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRF 240
           K YSA QWLVFYRVYNMSL+GNGLIDGRGQKWWDLPCKPHK               AIRF
Sbjct: 181 KGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHKSKSVSFFWQ------AIRF 240

Query: 241 FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFN 300
           FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYID +HIT+PALSPNTDGIHIENTN VQIFN
Sbjct: 241 FMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFN 300

Query: 301 SVVANGDDCISIGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD 360
           S+VANGDDCISIGSGSYNV+I NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD
Sbjct: 301 SIVANGDDCISIGSGSYNVEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSD 360

Query: 361 NGVRIKTWQGGYGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVD 420
           NGVRIKTWQGGYGAVRGVSFNNINM+NVRNPIIIDQFYCLNKGCLNQTSAV+VSDI+Y+D
Sbjct: 361 NGVRIKTWQGGYGAVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYID 420

Query: 421 IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPV 480
           IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKG+ILLDPFCWNAYGNLQTLTIPP+
Sbjct: 421 IKGTYDIRSPPMHFGCSDSMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPI 480

Query: 481 ACLLESSSRSILESEKDYC 489
           +CLLE+SSRS LESEKDYC
Sbjct: 481 SCLLETSSRSFLESEKDYC 489

BLAST of HG10019364.1 vs. ExPASy TrEMBL
Match: A0A6J1FAK7 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 6.5e-242
Identity = 410/488 (84.02%), Postives = 438/488 (89.75%), Query Frame = 0

Query: 7   FSSFSFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQPPSPSP------S 66
           F SF+ CIT IIFF    FPVHGRWHNHAK HK HSHHH H FTISQPPSPSP      S
Sbjct: 5   FCSFALCITLIIFF----FPVHGRWHNHAKNHK-HSHHHPH-FTISQPPSPSPSPSPPLS 64

Query: 67  PSSEPTGPPDDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDND 126
           PS EP  PPDD++GGS S N+T  FDVRH+GAIGDGTTDDT AFKMAWDTACQ DND   
Sbjct: 65  PSPEPANPPDDNDGGS-SFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDND--- 124

Query: 127 IDNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVF 186
              DYAVILVPY FSFMIQSTIFTGPCK+GIVFQVDGT +APDGPDAWPKSYSA QWLVF
Sbjct: 125 ---DYAVILVPYGFSFMIQSTIFTGPCKHGIVFQVDGTFIAPDGPDAWPKSYSAHQWLVF 184

Query: 187 YRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRVEGV 246
           YRV NMSLRGNG+IDGRGQKWWDLPCKPH+G KG T  G CDSPIAIRFFMSS L VEG+
Sbjct: 185 YRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGL 244

Query: 247 RIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCIS 306
           RIKDSPQFHFRFDNC+DVYID + ITAPA SPNTDGIHIENTN VQI NSVVANGDDCIS
Sbjct: 245 RIKDSPQFHFRFDNCRDVYIDSLTITAPAWSPNTDGIHIENTNGVQILNSVVANGDDCIS 304

Query: 307 IGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG 366
           IGSGSYNVDI+NITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG
Sbjct: 305 IGSGSYNVDIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG 364

Query: 367 YGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIRSPP 426
           +GAVRGVSFNNI+M+NVRNPIIIDQFYCLNKGCLNQTSAV+VSDI+Y+DIKG+YDIRSPP
Sbjct: 365 FGAVRGVSFNNIHMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGSYDIRSPP 424

Query: 427 MHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESSSRSI 486
           MHFGCSDSMPC+NLTLANIELFPSKG+I LDPFCWNAYG+LQTLTIPP+ CLLE+SSRSI
Sbjct: 425 MHFGCSDSMPCVNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASSRSI 479

Query: 487 LESEKDYC 489
           LE+++DYC
Sbjct: 485 LETQRDYC 479

BLAST of HG10019364.1 vs. ExPASy TrEMBL
Match: A0A6J1J721 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481948 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.6e-238
Identity = 404/490 (82.45%), Postives = 440/490 (89.80%), Query Frame = 0

Query: 9   SFSFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQPPSP----------S 68
           SF+ CI  IIFF    FPVHGRWHNHA  HK HSHHH H+FTISQPPSP          S
Sbjct: 7   SFALCIILIIFF----FPVHGRWHNHA-NHK-HSHHH-HHFTISQPPSPSTSASASTSTS 66

Query: 69  PSPSSEPTGPPDDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDD 128
           PSPS EP  PPDD++GGS S NST  FDVRH+GAIGDGTTDDT AFKMAWDTACQ D+ +
Sbjct: 67  PSPSPEPANPPDDNDGGS-SFNSTGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDDKN 126

Query: 129 NDIDNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWL 188
           N+ DN +AVILVPY FSFMIQSTIF+GPCK+G+VFQVDGT++APDGPD WPKSYSA QWL
Sbjct: 127 NNNDN-HAVILVPYGFSFMIQSTIFSGPCKHGVVFQVDGTLIAPDGPDTWPKSYSAHQWL 186

Query: 189 VFYRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRVE 248
           VFYRV NMSLRGNG+IDGRGQKWWDLPCKPH+G KG T  G CDSPIAIRFFMSS L VE
Sbjct: 187 VFYRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGSTKAGPCDSPIAIRFFMSSNLSVE 246

Query: 249 GVRIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDC 308
           G+RIKDSPQFHFRFDNC+DVYID ++ITAPALSPNTDGIHIENTN VQI NSVVANGDDC
Sbjct: 247 GLRIKDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVANGDDC 306

Query: 309 ISIGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQ 368
           ISIGSGSYNVDI+NITCGPGHGISIGSLGNHNS ACVSNITVRDSIIKVSDNGVRIKTWQ
Sbjct: 307 ISIGSGSYNVDIQNITCGPGHGISIGSLGNHNSLACVSNITVRDSIIKVSDNGVRIKTWQ 366

Query: 369 GGYGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIRS 428
           GG+GAVRGVSFNNI+M+NVRNPIIIDQFYCLNKGCLNQTSAV+VSDI+Y+DIKG+YDIRS
Sbjct: 367 GGFGAVRGVSFNNIHMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGSYDIRS 426

Query: 429 PPMHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESSSR 488
           PPMHFGCSDSMPC+NLTLANIELFPSKG+I LDPFCWNAYG+LQTLTIPP+ CLLE+SSR
Sbjct: 427 PPMHFGCSDSMPCVNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASSR 486

BLAST of HG10019364.1 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 637.5 bits (1643), Expect = 8.8e-183
Identity = 305/489 (62.37%), Postives = 380/489 (77.71%), Query Frame = 0

Query: 16  FIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDH------YFTISQPPSPSPSPSS------ 75
           F+     L+     R H+H +KHK ++H+H          +ISQPP+P P P        
Sbjct: 14  FMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSL 73

Query: 76  --EPTGPPDDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDNDI 135
              P+  P DDN G        I++VR +GA+GDG TDDT AFK AWD++C   N++N+ 
Sbjct: 74  PPSPSDDPADDNNG--------IYNVRKYGAVGDGETDDTEAFKTAWDSSC---NNENNT 133

Query: 136 DNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVFY 195
           D   +V+LVPY ++FMIQSTIFTGPC++   FQVDGT++ PDGP++WP + S RQWLVFY
Sbjct: 134 D---SVLLVPYGYTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFY 193

Query: 196 RVYNMSLRGNGLIDGRGQKWWDLPCKPHKGV-KGGTMTGACDSPIAIRFFMSSKLRVEGV 255
           RV  M+L+G G+IDGRGQKWWDLPCKPH+ V K   +TG CDSPIA+RFFMSS LRVEG+
Sbjct: 194 RVNGMALKGEGVIDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGL 253

Query: 256 RIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCIS 315
           +IK+SPQFHFRFD C+ V+++ +HITAP LSPNTDGIHIEN+N+V I+NS+++NGDDC+S
Sbjct: 254 QIKNSPQFHFRFDGCQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVS 313

Query: 316 IGSGSYNVDIKNITCGP-GHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQG 375
           IGSGSY+VDI+N+TCGP GHGISIGSLGNHNSRACVSNITVRDS+IK SDNGVRIKTWQG
Sbjct: 314 IGSGSYDVDIRNLTCGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQG 373

Query: 376 GYGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIRSP 435
           G G+V GV+FNNI++++VRNPIIIDQ+YC+ K C N+TSAV VSDI Y  IKGTYDIRSP
Sbjct: 374 GSGSVSGVTFNNIHVDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSP 433

Query: 436 PMHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESSSRS 489
           PMHFGCSD++PC NLTL++IEL P+KGEI+LDPFCWNAYG  + L+IPPV CL+    + 
Sbjct: 434 PMHFGCSDAVPCTNLTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKG 488

BLAST of HG10019364.1 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 555.1 bits (1429), Expect = 5.8e-158
Identity = 256/421 (60.81%), Postives = 327/421 (77.67%), Query Frame = 0

Query: 55  PSPSPSPSSEPTGPPDDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQW 114
           PSP+P+P+       D+DN   T      +FDV  FGAIGD +TDDT+AFKMAWD AC  
Sbjct: 29  PSPAPNPAYN-----DNDNIAPT------VFDVTSFGAIGDCSTDDTSAFKMAWDAACMS 88

Query: 115 DNDDNDIDNDYAVILVPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSA 174
               +      A++LVPY F F+++ T F GPC+  +V Q+DG +++PDGP +WP +Y  
Sbjct: 89  TGPKS------ALLLVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQ- 148

Query: 175 RQWLVFYRVYNMSLRGNGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSK 234
           RQW++FYRV  +S++G+G+I+GRGQKWW+LPCKPHKG+ G T TG CDSP+AIR F SSK
Sbjct: 149 RQWMMFYRVNGLSIQGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSK 208

Query: 235 LRVEGVRIKDSPQFHFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVAN 294
           +R++G+   +S QFH RFDNC DV +D + I APA SPNTDGIHIENT+ VQI NS+++N
Sbjct: 209 VRIQGINFMNSAQFHVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISN 268

Query: 295 GDDCISIGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRI 354
           GDDCISIG+G +NVDIKN+TCGP HGISIGSLG HNS+A VSNITV +S I  SDNGVRI
Sbjct: 269 GDDCISIGAGCFNVDIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRI 328

Query: 355 KTWQGGYGAVRGVSFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTY 414
           KTWQGG G+V  + F+NI M NVRNPI+IDQ+YC    C NQTSAV++SD+ Y +IKGTY
Sbjct: 329 KTWQGGSGSVSRIVFSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTY 388

Query: 415 DIRSPPMHFGCSDSMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLE 474
           D+RSPP+HFGCSDS+PC NLTL  ++LFPSKG+ L +PFCWNAYG+++ +T+PPV CLL+
Sbjct: 389 DLRSPPIHFGCSDSVPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLD 430

Query: 475 S 476
           +
Sbjct: 449 A 430

BLAST of HG10019364.1 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 551.2 bits (1419), Expect = 8.3e-157
Identity = 266/481 (55.30%), Postives = 343/481 (71.31%), Query Frame = 0

Query: 13  CIT---FIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQPPSPSPSPSSEPTGPP 72
           CIT    II F  L +    R H+ A                SQPPSPSP+P+      P
Sbjct: 11  CITTLILIITFSLLSYGTEARLHHQA----------------SQPPSPSPNPND-----P 70

Query: 73  DDDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDNDIDNDYAVIL 132
                 S  ++   ++DVR +GA+G+G  DDT +FK AWD+AC      N+ +N  +V+ 
Sbjct: 71  SKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSAC-----SNNKNNTASVLH 130

Query: 133 VPYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVFYRVYNMSLR 192
           VPY F+FMI+STIFTGPC++   FQVDGT++  DGP +WP S + RQWL FYR+  M+L+
Sbjct: 131 VPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQ 190

Query: 193 GNGLIDGRGQKWWDLPCKPH-KGVKGGTMTGACDSPIAIRFFMSSKLRVEGVRIKDSPQF 252
           G G+IDGRGQ WWDLPCKPH + V    + G C+SP A+RFFMSS + V+G+ IK+SPQ 
Sbjct: 191 GAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFMSSNVIVKGLSIKNSPQV 250

Query: 253 HFRFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCISIGSGSYNV 312
           H + D C  V+I+ + I +P  SPNTDGIHIEN+N+V+I+NSV++NGDDC+SIG G+Y++
Sbjct: 251 HLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSVISNGDDCVSIGPGAYDI 310

Query: 313 DIKNITCGP-GHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGV 372
           DI+NITCGP GHGISIGSLG  NS ACVSN+TVRDS IK S+NGVRIKTWQGG G+V GV
Sbjct: 311 DIRNITCGPGGHGISIGSLGEKNSHACVSNVTVRDSFIKFSENGVRIKTWQGGSGSVSGV 370

Query: 373 SFNNINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIRSPPMHFGCSD 432
           +F+NI+++ VRNPIIIDQ+YC  K C N+TSAV V+DI Y  IKGTYDIRSPPMHFGCS+
Sbjct: 371 TFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYDIRSPPMHFGCSN 430

Query: 433 SMPCMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESSSRSILESEKDY 489
           ++PC NLTL+NIEL PSK +I++ PFCWNAYG     ++P ++CL  + S S+L      
Sbjct: 431 NVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLKSNPSTSLLSGLSGR 465

BLAST of HG10019364.1 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 521.2 bits (1341), Expect = 9.2e-148
Identity = 247/466 (53.00%), Postives = 326/466 (69.96%), Query Frame = 0

Query: 17  IIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHYFTISQPPSPSP------SPSSEPTGPPD 76
           I+  + +   + G + N A+  + H H   H  T   PP P P       P   P+ P  
Sbjct: 7   ILVIYIISTVLFGHFTNVARA-RYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYP 66

Query: 77  DDNGGSTSINSTSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDNDIDNDYAVILV 136
             +      +S  +FDV  FGA+GDG+ DDTAAF+ AW  AC  ++          V+L 
Sbjct: 67  SPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVES---------GVVLA 126

Query: 137 PYDFSFMIQSTIFTGPCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVFYRVYNMSLRG 196
           P    F I STIF+GPCK G+VFQ+DG +M PDGP+ WP+  +  QWLVFYR+   +  G
Sbjct: 127 PEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSG 186

Query: 197 NGLIDGRGQKWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRVEGVRIKDSPQFHF 256
            G ++G GQKWWDLPCKPH+G  G + +G C SP  IRFFMS+ + V+G+RI++SPQFH 
Sbjct: 187 KGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHM 246

Query: 257 RFDNCKDVYIDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCISIGSGSYNVDI 316
           +FD C+ V I+ I I++P LSPNTDGIH+ NT +V I+NSVV+NGDDCISIG+G  +VDI
Sbjct: 247 KFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDI 306

Query: 317 KNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFN 376
           + +TCGP HGISIGSLG HNS+ACVSNITVR+++I+ SDNG+R+KTWQGG G+V  + F 
Sbjct: 307 QGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFE 366

Query: 377 NINMENVRNPIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIRSPPMHFGCSDSMP 436
           NI MENV N II+DQ+YC +K C N+TSAV V D+ Y +IKGTYD+RSPP+HF CSD++ 
Sbjct: 367 NIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVA 426

Query: 437 CMNLTLANIELFPSKGEILLDPFCWNAYGNLQTLTIPPVACLLESS 477
           C N+T++ +EL P +GE++ DPFCWNAYG  +TLTIPP+ CLL+ S
Sbjct: 427 CTNITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGS 462

BLAST of HG10019364.1 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 371.7 bits (953), Expect = 9.0e-103
Identity = 187/411 (45.50%), Postives = 258/411 (62.77%), Query Frame = 0

Query: 81  STSIFDVRHFGAIGDGTTDDTAAFKMAWDTACQWDNDDNDIDNDYAVILVPYDFSFMIQS 140
           S+   +V   GA GDGT+DDT AF+ AW  AC+            + +LVP   +F++  
Sbjct: 47  SSGTINVLDHGAKGDGTSDDTKAFEDAWQVACK---------VAASTLLVPSGSTFLVGP 106

Query: 141 TIFTG-PCKNGIVFQVDGTVMAPDGPDAWPKSYSARQWLVFYRVYNMSLRGNGLIDGRGQ 200
             F G  CK  IVFQ++G ++AP    AW       QW+ F  +  ++++G G+IDGRG 
Sbjct: 107 VSFLGKECKEKIVFQLEGKIIAPTSASAW--GSGLLQWIEFKALQGITIKGKGIIDGRGS 166

Query: 201 KWWDLPCKPHKGVKGGTMTGACDSPIAIRFFMSSKLRVEGVRIKDSPQFHFRFDNCKDVY 260
            WW+        + G  M      P A+RF+ S+ + V G+ I++SPQ H +FDNC  + 
Sbjct: 167 VWWN-------DMMGTKMPRT--KPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQ 226

Query: 261 IDGIHITAPALSPNTDGIHIENTNAVQIFNSVVANGDDCISIGSGSYNVDIKNITCGPGH 320
           +     ++P  SPNTDGIH++N+    I+ S +A GDDCISI +G  N++I ++ CGPGH
Sbjct: 227 VSDFTTSSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGH 286

Query: 321 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFNNINMENVRN 380
           GISIG LG  N++ACVSNITVRD  +  + NGVRIK+WQGG G+V+ V F+NI + NV N
Sbjct: 287 GISIGGLGKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVAN 346

Query: 381 PIIIDQFYCLNKGCLNQTSAVLVSDITYVDIKGTYDIRSPPMHFGCSDSMPCMNLTLANI 440
           PIIIDQ+YC   GC N+TSAV VS+I Y++IKGTY     P+ F CSDS+PC  ++L+ I
Sbjct: 347 PIIIDQYYCDGGGCHNETSAVAVSNINYINIKGTY--TKEPVRFACSDSLPCTGISLSTI 406

Query: 441 ELFPSKGEI-LLDPFCWNAYGNLQTLTIPPVACL-LESSSRSILESEKDYC 489
           EL P+ G+   LDPFCW A+G L+T T+PP+ CL  E S  +   S  D C
Sbjct: 407 ELKPATGKASSLDPFCWKAHGELKTKTLPPIQCLKTEKSPEAASRSNNDAC 435