HG10012827.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10012827.1 vs. NCBI nr
Match: KAG6582408.1 (Protein NRT1/ PTR FAMILY 5.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1727.6 bits (4473), Expect = 0.0e+00
Identity = 885/1207 (73.32%), Postives = 1018/1207 (84.34%), Query Frame = 0

Query: 4    AAADQETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIY 63
            A+A +E+G+DDYTKDGTVD KGNPVLRSK G WKACSFI+VYE+ ERM + GI+ NLII+
Sbjct: 3    ASAAEESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNLIIF 62

Query: 64   LTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTL 123
            LT KL+QGT+ ++NNVTNW+GTVWI PILGAY+ADA+LGRYRTF I+S +CL  M LLTL
Sbjct: 63   LTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGLLTL 122

Query: 124  AVSVPSLKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDD 183
            AVS+PSLKPPPCL+ INK NCK AS LQLAVFFG+LY+LA+ +GGTKPNIST+GADQFD+
Sbjct: 123  AVSLPSLKPPPCLD-INKGNCKAASTLQLAVFFGALYMLALGTGGTKPNISTIGADQFDE 182

Query: 184  FDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVV 243
            F PKEKAQKLSFFNWW+FS+F G LFA+TILVYIQDNVGWSLGYG+PTIGL ++ILIFV 
Sbjct: 183  FHPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVA 242

Query: 244  GTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTP 303
            GTPFYRH+LP GSPF +MA+VIVAA  NWRLPLPNDP +L+EL  +              
Sbjct: 243  GTPFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFE-------------- 302

Query: 304  SLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQG 363
               FLNKAAIRRGSSD W+LCTVT+VEETKQM+RMIP++ICTF+PSTM+AQ+HTLFIKQG
Sbjct: 303  --EFLNKAAIRRGSSDSWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLFIKQG 362

Query: 364  TTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIG 423
            TTLDRSIGSHF++PPASL AFVTISMLLS++IYDR+FVK+MQ++TKNPRGIT+LQRMGIG
Sbjct: 363  TTLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKVMQRITKNPRGITLLQRMGIG 422

Query: 424  MICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIA 483
            MI H+L+M +AS+VE+HRL++A +NGS     ++ LPLTIF LLPQF+L GVADAF ++A
Sbjct: 423  MILHVLIMIIASRVERHRLDVARQNGS-----KQELPLTIFTLLPQFMLVGVADAFTEVA 482

Query: 484  SNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHL 543
              EFFYDQAPE+MKSLG+SY MTS+GIGNFLS+F+LS VS IT ++G GWI+NNLN SHL
Sbjct: 483  KIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKRGNGWIMNNLNASHL 542

Query: 544  DYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSK------------ 603
            DY+YA LAV+SA+NFFLFLLISK YVYKAEVS SIK L D+LK KK K            
Sbjct: 543  DYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKALADQLKDKKLKPFVEVRFWTSSP 602

Query: 604  ----------------------------GLQQTDQESMVDDYTKDGTVDLKGKPLLRSKT 663
                                        G    DQES +DDYTKDGTVD KG P LRS T
Sbjct: 603  LLFFPKAQLGLGSFLMGSASTQFLKPINGGSSRDQESGLDDYTKDGTVDRKGNPTLRSNT 662

Query: 664  GAWKACSFIIVYELMEKIMFHGIAANLIIYLTTKLHQGTVTASNNVTNWSGTVWIMPILG 723
            G WKACSFI+VYEL++++MF+GIAANLIIYLTTKL+QGTVTASNNVTNW+GTVWI PI G
Sbjct: 663  GGWKACSFIVVYELIDRMMFNGIAANLIIYLTTKLNQGTVTASNNVTNWTGTVWITPIFG 722

Query: 724  AYIADAHLGRYRTFLISSFIWFTAMSLLTLAVSVPSLKPPPCLEAITKQNCKQASKLQLA 783
            AY+ADAHLG YRTF ISS   F AMSLLT+AVSVPSL+PPPCLE  +K+NCKQASKLQLA
Sbjct: 723  AYVADAHLGCYRTFFISSLASFMAMSLLTVAVSVPSLQPPPCLEP-SKENCKQASKLQLA 782

Query: 784  VFFGSLYLLALASGGTKPNISTMGADQFDDFDPKEKAQKLSFFNWWLFSVFSGFLFASTV 843
            VFFGSLY+LA+ASGGTKPNISTMGADQFDDF PKEK+QKLSFFNWW+FSVFSG LFAST+
Sbjct: 783  VFFGSLYMLAVASGGTKPNISTMGADQFDDFHPKEKSQKLSFFNWWMFSVFSGILFASTI 842

Query: 844  LVYIQDNVGWSLGYGIPTIGIAIAILIFVVGTPFYRHRLPN-GSPFTSMANVIVAAALNW 903
            LVYIQDNVGWS GYGIPTIG+ +AILIFV GTPFYRHRLP+ GSPF  MA VIVAAA NW
Sbjct: 843  LVYIQDNVGWSFGYGIPTIGLGVAILIFVAGTPFYRHRLPSGGSPFIRMARVIVAAARNW 902

Query: 904  RLPLPNHPNQLHELDLHHYSKPGTFKIDSTPSLRFLNKAAIRRDSSDPWRLCTVTEVEET 963
            R+PLPN PNQL+EL++  YS     KIDSTPS RFLNKAA+R  SS PWR CTVT+VEET
Sbjct: 903  RVPLPNDPNQLYELEVQQYS-----KIDSTPSFRFLNKAAVRTGSSHPWRSCTVTQVEET 962

Query: 964  KQMLRMIPILICTFIPNTIMAQTHTLFIKQGTTLNRSIGSHFKIPPASLNVFVTISMLLS 1023
            KQMLRMIPILICTFIP+T++AQ+HTLFIKQGTTL+R+IGSHFK+PPASL  FVTISMLLS
Sbjct: 963  KQMLRMIPILICTFIPSTMVAQSHTLFIKQGTTLDRTIGSHFKVPPASLYAFVTISMLLS 1022

Query: 1024 ILIYDRIFVK-MQRVTKNPRGITMLQRMGIGMICLVLVMTVASRVEKHRLKIVAA---TE 1083
            I+IYDRIFVK MQRVT+NPRGITMLQRMGIGMI  VLVMTVASRVEK RL +  A     
Sbjct: 1023 IVIYDRIFVKIMQRVTRNPRGITMLQRMGIGMIFHVLVMTVASRVEKRRLHVARANGLVR 1082

Query: 1084 NGSSAQVLPLTIFILLPQFILTGFAEAFVQVAVMEFFYDQAPENMKSLGTSYTMTSLGIG 1143
            NG S QVLPL+IF LLPQF+LTG A+A +Q+A +EFFYDQAP++MKSLG+SY MTSLGIG
Sbjct: 1083 NG-SGQVLPLSIFTLLPQFMLTGVADALLQIANVEFFYDQAPKSMKSLGSSYMMTSLGIG 1142

Query: 1144 NFLSSLILSKVSEITKRQGKGWILNNLNASHLDYFYALLAVMSAVNFFLFLLISKLYVYK 1166
            NFLSS +LSKVSEITKR G+GWILNNLNASHLDYFYALLA MS VNFF+FL IS+LYVY+
Sbjct: 1143 NFLSSFVLSKVSEITKRHGEGWILNNLNASHLDYFYALLAAMSGVNFFVFLGISQLYVYR 1180

BLAST of HG10012827.1 vs. NCBI nr
Match: RYR52937.1 (hypothetical protein Ahy_A06g027793 [Arachis hypogaea])

HSP 1 Score: 1378.2 bits (3566), Expect = 0.0e+00
Identity = 679/1193 (56.92%), Postives = 912/1193 (76.45%), Query Frame = 0

Query: 3    GAAADQETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLII 62
            G    +E G +DYT+DGTVD KG PVLRS TG WKACSFI+ YE++ERM + GIA+NL++
Sbjct: 2    GVLLSEEKG-EDYTEDGTVDLKGRPVLRSNTGKWKACSFIVGYEMVERMAYYGIASNLVV 61

Query: 63   YLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLT 122
            YLT +L++GT+ +S NVTNW G VW  P +GAY+ADAYLGRY TF ISS + L+ M LLT
Sbjct: 62   YLTKELHEGTVKSSKNVTNWVGVVWFMPAIGAYIADAYLGRYSTFLISSAIYLLGMCLLT 121

Query: 123  LAVSVPSLKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFD 182
            LAVS+P+LKPPPC +    ++C++A+ LQ+ +FF  LY++A+ +GGTKPNISTMGADQFD
Sbjct: 122  LAVSLPALKPPPCPQ---DKDCQKATSLQVGLFFLGLYIIAVGTGGTKPNISTMGADQFD 181

Query: 183  DFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFV 242
             F+PKEKAQK+SFFNWW+  +  G +F++T+LVYIQDNVGW+LGYGIPT GL  +IL+F+
Sbjct: 182  KFEPKEKAQKISFFNWWVTFILIGTIFSNTVLVYIQDNVGWALGYGIPTGGLLFSILVFL 241

Query: 243  VGTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDST 302
             GTPFYRH+ P+GSP  RM  VIVAA   W+L +P+DP +LYEL ++ Y+ NG  +I  +
Sbjct: 242  FGTPFYRHKSPSGSPLTRMLQVIVAAVRKWKLEVPDDPKELYELTVEEYAINGRNRIYHS 301

Query: 303  PSLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQ 362
            PSL FL+KAAI+   + PW LCT+T+VEETKQM++M+PIM+ T +PST++AQ++TLFIKQ
Sbjct: 302  PSLSFLDKAAIKTKQTQPWMLCTMTQVEETKQMMKMVPIMVTTCMPSTVIAQANTLFIKQ 361

Query: 363  GTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGI 422
            GTTLDRSIG +FK+PPA L AF+ I MLLS++ YDR+ V ++++ TKNPRGIT+LQR+GI
Sbjct: 362  GTTLDRSIGPNFKIPPACLTAFINIFMLLSVVTYDRVLVPLVRRYTKNPRGITLLQRLGI 421

Query: 423  GMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQI 482
            G++ HI++M  A   EK RL++A ++  +L  +  +LPL+IFILLPQF L G+AD F+ +
Sbjct: 422  GLVIHIVIMITACLAEKKRLSVARQH--NLLGQHDILPLSIFILLPQFALAGIADTFVDV 481

Query: 483  ASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG--KGWILNNLNV 542
            A  + FYDQAPE MKSLG+SY   SL IG F S+F++S V+++TKR    KGWIL+NLNV
Sbjct: 482  AKLDLFYDQAPEGMKSLGTSYVFISLSIGTFFSSFLISTVADLTKRNNGQKGWILDNLNV 541

Query: 543  SHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDS----IKLLTDELKKKKSKGLQQT 602
            SHLDY++A LA++SA+NF  FL+ +K +VY  + + +    +++  +         L Q 
Sbjct: 542  SHLDYYFAFLAILSAINFLCFLVAAKFFVYNNDATQASIIGLEMKNNNASSHDKMELNQK 601

Query: 603  DQ--ESMVDDYTKDGTVDLKGKPLLRSKTGAWKACSFIIVYELMEKIMFHGIAANLIIYL 662
            ++    + +DYT+DGTVDLKG+P+LRSKTG WKACSFI+ YE+ E++ ++GIA+NL+ YL
Sbjct: 602  EKAPARLEEDYTQDGTVDLKGRPVLRSKTGKWKACSFIVGYEVFERMAYYGIASNLVQYL 661

Query: 663  TTKLHQGTVTASNNVTNWSGTVWIMPILGAYIADAHLGRYRTFLISSFIWFTAMSLLTLA 722
            T KLH+G V +SNNV+NW G+VW+ P+ GAYIADA+LGRY TFLISS I+   M L+TLA
Sbjct: 662  TEKLHEGIVNSSNNVSNWVGSVWMTPLAGAYIADAYLGRYWTFLISSAIYLLGMVLITLA 721

Query: 723  VSVPSLKPPPCLEAITKQNCKQASKLQLAVFFGSLYLLALASGGTKPNISTMGADQFDDF 782
            VSV +L+PPPC   +   NC +A+KLQL +FF +LY +A+ +GGTKPNISTMGADQFD+F
Sbjct: 722  VSVRALRPPPCPVGVDDANCPRATKLQLGIFFLALYTIAVGTGGTKPNISTMGADQFDEF 781

Query: 783  DPKEKAQKLSFFNWWLFSVFSGFLFASTVLVYIQDNVGWSLGYGIPTIGIAIAILIFVVG 842
            +PKE+  KLSFFNWW+FS+F G LF++T LVYIQ+ V W++GYG+PTIG+A++IL+F+ G
Sbjct: 782  EPKERHHKLSFFNWWMFSIFFGTLFSNTFLVYIQEKVSWTIGYGLPTIGLAVSILVFLFG 841

Query: 843  TPFYRHRLPNGSPFTSMANVIVAAALNWRLPLPNHPNQLHELDLHHYSKPGTFKIDSTPS 902
            TPFYRH+LP+GSP T +  V VAA   W++ +P  P +LHEL +  Y   G  +ID +PS
Sbjct: 842  TPFYRHKLPSGSPITRILQVYVAAFRKWKVHIPGDPKELHELSIEEYVSNGRTRIDHSPS 901

Query: 903  LRFLNKAAIRRDSSDPWRLCTVTEVEETKQMLRMIPILICTFIPNTIMAQTHTLFIKQGT 962
              FL+KAA R D + PW LCTVT+VEETKQM +M+PILI T +P+T++ Q  TLFIKQG 
Sbjct: 902  FSFLDKAATRTDQTSPWMLCTVTQVEETKQMTKMVPILITTLLPSTMLIQATTLFIKQGN 961

Query: 963  TLNRSIGSHFKIPPASLNVFVTISMLLSILIYDRIFVK-MQRVTKNPRGITMLQRMGIGM 1022
            TLNRS+G  F IPPA L  F+TI ML+SI+IYDR+FV  ++R TKNPRGIT+LQR+GIG+
Sbjct: 962  TLNRSMGPDFDIPPACLTSFITIFMLISIVIYDRVFVPVIRRYTKNPRGITLLQRLGIGL 1021

Query: 1023 ICLVLVMTVASRVEKHRLKIVAATENGSSAQVLPLTIFILLPQFILTGFAEAFVQVAVME 1082
            +  ++V+  AS VE+ RL +            LPLTIFILLPQF LTG A+ FV+VA +E
Sbjct: 1022 VIHIIVLITASFVERKRLSVAREHNLLRQHDQLPLTIFILLPQFALTGIADNFVEVAKLE 1081

Query: 1083 FFYDQAPENMKSLGTSYTMTSLGIGNFLSSLILSKVSEITKRQG-KGWILNNLNASHLDY 1142
            FFYDQAPE MKS+GTSY  TSLGIG+FL++ +L+ V+ +TKR G KGW+LNNLN SHLDY
Sbjct: 1082 FFYDQAPEGMKSMGTSYFTTSLGIGSFLATFLLTTVANLTKRNGHKGWVLNNLNVSHLDY 1141

Query: 1143 FYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIRLLTDELKKKKSKASSNSQV 1186
            +YA +A +S +N   FL+++K +VY  +V+     L  E+    S+  SN+++
Sbjct: 1142 YYAFMAGLSFINLLCFLVVAKFFVYNDDVAQKKTGL--EMNTASSQGYSNNRI 1186

BLAST of HG10012827.1 vs. NCBI nr
Match: RDX80312.1 (Protein NRT1/ PTR FAMILY 5.2, partial [Mucuna pruriens])

HSP 1 Score: 1374.0 bits (3555), Expect = 0.0e+00
Identity = 677/1202 (56.32%), Postives = 911/1202 (75.79%), Query Frame = 0

Query: 10   TGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLN 69
            +G +DYT+DGTVD KG PVLRS TG W+ACSFI+ YE+IERM + GIA+NL++YLT KL+
Sbjct: 11   SGREDYTQDGTVDLKGRPVLRSNTGRWRACSFIVGYEMIERMAYYGIASNLVLYLTKKLH 70

Query: 70   QGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPS 129
            +GT+ +SN+VTNW GTVW+ P  GAY+ADAYLGRY TF I+S + L+ M LLTL VS+P+
Sbjct: 71   EGTVKSSNHVTNWVGTVWMMPAAGAYIADAYLGRYSTFVIASAIYLLGMCLLTLTVSLPA 130

Query: 130  LKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEK 189
            LKPPPC   +  ++C++AS LQ+ +FF +LY++A  +GGTKPNISTMGADQFD+F+P+E+
Sbjct: 131  LKPPPCALGVADKDCQRASSLQVGIFFCALYIIAAGTGGTKPNISTMGADQFDEFEPRER 190

Query: 190  AQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYR 249
            +QKLSF+NWW+F++  G +FA T+LVYIQD VG+ LGYGIPTIGL ++IL+F++GTP YR
Sbjct: 191  SQKLSFYNWWVFNILIGTIFAQTLLVYIQDKVGFGLGYGIPTIGLALSILVFLLGTPLYR 250

Query: 250  HRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDL-QHYSKNGSFKIDSTPSLR-- 309
            HRLP+GSP  RM  V VAA   W++ +P+D N+L+EL + ++Y+  G  +I  + SLR  
Sbjct: 251  HRLPSGSPLTRMVQVFVAAMTKWKVHVPDDVNELHELSIEEYYASKGRSRIYHSSSLRLH 310

Query: 310  -----------FLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQS 369
                       FL+KAA++ G +  W LCTVT+VEETKQM++MIPI+I T +PST++AQ+
Sbjct: 311  NNLITLTNVSSFLDKAAVKTGQTSQWMLCTVTQVEETKQMMKMIPILITTCVPSTIIAQT 370

Query: 370  HTLFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGIT 429
             TLFI+QGTTLDR +G HF++PPA L AFV I ML+S++IYDR FV  +++ TK+PRGI+
Sbjct: 371  STLFIRQGTTLDRRMGPHFQIPPACLIAFVNIFMLISVVIYDRFFVPSIRRYTKDPRGIS 430

Query: 430  MLQRMGIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGV 489
            +LQR+GIG++ H+++M  A  VE+ RL +A EN   L ++   +PLTIFILLPQF LTG+
Sbjct: 431  LLQRLGIGLVLHVIIMLTACLVERKRLGVAREN--HLLEQNDTIPLTIFILLPQFALTGI 490

Query: 490  ADAFLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG-KGWI 549
            AD F+ +A  EFFYDQAPE+MKSLG+SYF T+L IGNFLSTF+LS V+++T+R G KGWI
Sbjct: 491  ADTFVDVAKLEFFYDQAPESMKSLGTSYFTTTLSIGNFLSTFLLSTVADLTRRNGHKGWI 550

Query: 550  LNNLNVSHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDS-IKLLTDELKKKKSKGL 609
            L+NLNVS LDY+YA LA++SA+N   F++++KLYVY  +V+ + + L  +    K + G+
Sbjct: 551  LDNLNVSRLDYYYAFLAMLSAINLLCFVVVAKLYVYNVDVTQTKMDLDMNPASSKDNNGI 610

Query: 610  QQTDQE---------------SMVDDYTKDGTVDLKGKPLLRSKTGAWKACSFII----- 669
             Q+  +               S  +DYT+DGTVDL G+PLLRSKTG WKACSFI+     
Sbjct: 611  SQSTPQPDAKLMAVVEEKGPASGNEDYTQDGTVDLMGRPLLRSKTGRWKACSFIVGYEYG 670

Query: 670  ---------VYELMEKIMFHGIAANLIIYLTTKLHQGTVTASNNVTNWSGTVWIMPILGA 729
                      YE+ E++ F+GI +NL++YLT KLH+GTV +SN+V+NW G+VW+MP+ GA
Sbjct: 671  YACVCTKSTGYEVFERMAFYGIQSNLVLYLTKKLHEGTVKSSNHVSNWVGSVWMMPLAGA 730

Query: 730  YIADAHLGRYRTFLISSFIWFTAMSLLTLAVSVPSLKPPPCLEAITKQNCKQASKLQLAV 789
            YIADA+LGRY TF+I+S I+   M LLTLAVS+P L+PPPC +    +NC +AS LQ  +
Sbjct: 731  YIADAYLGRYWTFVIASCIYVLGMCLLTLAVSLPVLRPPPCAQ---DENCPEASSLQYGI 790

Query: 790  FFGSLYLLALASGGTKPNISTMGADQFDDFDPKEKAQKLSFFNWWLFSVFSGFLFASTVL 849
            FF +LY +A+ +GGTKPNISTMGADQFD+F+PKE++ KLSFFNWW FS+F G LFA+T L
Sbjct: 791  FFTALYTIAIGTGGTKPNISTMGADQFDEFEPKERSHKLSFFNWWFFSIFFGTLFANTFL 850

Query: 850  VYIQDNVGWSLGYGIPTIGIAIAILIFVVGTPFYRHRLPNGSPFTSMANVIVAAALNWRL 909
            VYIQD VGW++GYG+PT+G+A+++L+F+VGTP+YRHRLP+GSP T +  V VAA   W+L
Sbjct: 851  VYIQDRVGWTIGYGLPTLGLAVSVLLFLVGTPYYRHRLPSGSPITRVLQVFVAAGRKWKL 910

Query: 910  PLPNHPNQLHELDLHHYSKPGTFKIDSTPSLRFLNKAAIRRDSSDPWRLCTVTEVEETKQ 969
             +P+ P +LHEL +  Y+  G  +ID + SL FLNKAAI+   +  W L TVT+VEETKQ
Sbjct: 911  KVPDDPKELHELSIEEYASSGRSRIDHSSSLSFLNKAAIKSGQTSAWMLSTVTQVEETKQ 970

Query: 970  MLRMIPILICTFIPNTIMAQTHTLFIKQGTTLNRSIGSHFKIPPASLNVFVTISMLLSIL 1029
            M +++PIL+ T IP+T+  QT T+F+KQG TL+R +G HF IPPA L  FVTISML++I+
Sbjct: 971  MTKLMPILLTTIIPSTLYVQTSTIFVKQGATLDRRMGPHFDIPPACLTAFVTISMLITIV 1030

Query: 1030 IYDRIFVKM-QRVTKNPRGITMLQRMGIGMICLVLVMTVASRVEKHRLKIVAATENGSSA 1089
            IYDR+FV + +R TKNPRGITMLQR+GIG++  V VM  A   E+ RL++V         
Sbjct: 1031 IYDRVFVPLIRRYTKNPRGITMLQRLGIGLVLHVTVMITACLAERRRLRVVRENHLFGPH 1090

Query: 1090 QVLPLTIFILLPQFILTGFAEAFVQVAVMEFFYDQAPENMKSLGTSYTMTSLGIGNFLSS 1149
              +PLTIFILLPQ+ L G A+ FV+VA ME FYDQAP  MKSLGT+Y  TSLG+G+FLSS
Sbjct: 1091 DTIPLTIFILLPQYALAGVADNFVEVAKMELFYDQAPYGMKSLGTAYFTTSLGVGSFLSS 1150

Query: 1150 LILSKVSEITKRQG-KGWILNNLNASHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVS 1165
             +LS V+ ITKR G  GW+L+NLN SHLDY+YA +AV+S +N   FL+++K +VY  +V+
Sbjct: 1151 FLLSTVANITKRHGHTGWVLDNLNVSHLDYYYAFMAVLSLLNLLCFLVVAKFFVYNVDVT 1207

BLAST of HG10012827.1 vs. NCBI nr
Match: QCD97765.1 (solute carrier family 15 [Vigna unguiculata])

HSP 1 Score: 1368.2 bits (3540), Expect = 0.0e+00
Identity = 664/1165 (57.00%), Postives = 904/1165 (77.60%), Query Frame = 0

Query: 14   DYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGTL 73
            DYTKDGT+D KG PVLRS TG W+ACSFI+ YE+IERM + GIA+NL++YLT KL++GT+
Sbjct: 17   DYTKDGTLDLKGKPVLRSNTGRWRACSFIVGYEMIERMAYYGIASNLVLYLTKKLHEGTV 76

Query: 74   TASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPSLKPP 133
             +SN+VTNW G VWI P  GAY+ADA+LGRY TF ISS + L+ M LLTLAVS+P L+PP
Sbjct: 77   KSSNHVTNWAGAVWIMPAAGAYIADAFLGRYWTFVISSAIYLLGMCLLTLAVSLPGLRPP 136

Query: 134  PCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKL 193
             C   I  ++C QAS LQ+ +FF +LY++A  +GGTKPNISTMGADQFD+F+PKE++QKL
Sbjct: 137  ACAPGIADQDCPQASSLQVGIFFFALYIIAAGTGGTKPNISTMGADQFDEFEPKERSQKL 196

Query: 194  SFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLP 253
            SF+NWW+F++  G + A T+LVYIQD VG+ LGYGIPTI L V+I +F++GTP YRHRLP
Sbjct: 197  SFYNWWVFNILIGTISAQTLLVYIQDRVGFGLGYGIPTIALAVSIFMFLLGTPLYRHRLP 256

Query: 254  NGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQH-YSKNGSFKIDSTPS-------L 313
            +GSP  RM  V+++A   W++ +P+D N+L+EL ++  Y+  G  +I  T          
Sbjct: 257  SGSPLTRMLQVLLSAVRKWKVHVPHDLNELHELSVEECYASKGRTRIQHTQEYCNLTNVC 316

Query: 314  RFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTT 373
            RFL+KAA++ G + PW LCTVT++EE KQM++M+PI+I T IPST++AQ+ TLFI+QGTT
Sbjct: 317  RFLDKAAVKTGETSPWMLCTVTQIEEAKQMMKMVPILITTCIPSTIIAQTTTLFIRQGTT 376

Query: 374  LDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMI 433
            LDR IG HF++PPA L AFV I ML+S++IYDR+FV  ++  TKNPRGI++LQR+GIG++
Sbjct: 377  LDRRIGPHFEIPPACLIAFVNIFMLISVVIYDRLFVPAIRHYTKNPRGISLLQRLGIGLV 436

Query: 434  CHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASN 493
             H+++M  A  VE+ RL++A E  + L Q  K+ PLTIFILLPQF LTG+AD F+ +A  
Sbjct: 437  LHVIIMLTACFVERKRLSVAREK-NLLGQLDKI-PLTIFILLPQFALTGIADTFVDVAKL 496

Query: 494  EFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG-KGWILNNLNVSHLD 553
            EFFYDQAPE MKSLG+SYF T+L IGNFL++F+LS V+++T R G K WIL+NLN S LD
Sbjct: 497  EFFYDQAPEAMKSLGTSYFTTTLSIGNFLNSFLLSTVADLTHRHGHKSWILDNLNASRLD 556

Query: 554  YFYALLAVMSAVNFFLFLLISKLYVY---KAEVSDSIKLLTDELKKKKSKGLQQTDQESM 613
            Y+YA LA++SA+NFF F+ ++KLYVY   + +++  + +  D  +       ++    + 
Sbjct: 557  YYYAFLALLSAINFFCFVAVAKLYVYNGDETQINKDLDMNPDSPQDNTEISQKEKGPANG 616

Query: 614  VDDYTKDGTVDLKGKPLLRSKTGAWKACSFIIVYELMEKIMFHGIAANLIIYLTTKLHQG 673
             +DYT+DGTVDLKG+P+LR++TG WKACSFI+ YE+ E++ F+GI +NL+IYLT KLH+G
Sbjct: 617  NEDYTQDGTVDLKGRPVLRTETGKWKACSFIVGYEVFERMAFYGIQSNLVIYLTRKLHEG 676

Query: 674  TVTASNNVTNWSGTVWIMPILGAYIADAHLGRYRTFLISSFIWFTAMSLLTLAVSVPSLK 733
            TV +SN+V+NW G+VW+MP+ GAYIADA LGRY+TF+I+S I+   M LLTLAVS+P+L+
Sbjct: 677  TVKSSNDVSNWVGSVWMMPLAGAYIADAFLGRYKTFIIASCIYVAGMCLLTLAVSLPALR 736

Query: 734  PPPCLEAITKQNCKQASKLQLAVFFGSLYLLALASGGTKPNISTMGADQFDDFDPKEKAQ 793
            PP C E    +NC +AS LQ  +FF +LY++A+ +GGTKPNISTMGADQFD+F+PKE++ 
Sbjct: 737  PPQCDEG---ENCPEASSLQYGIFFLALYIIAIGTGGTKPNISTMGADQFDEFEPKERSY 796

Query: 794  KLSFFNWWLFSVFSGFLFASTVLVYIQDNVGWSLGYGIPTIGIAIAILIFVVGTPFYRHR 853
            KLSFFNWW FS+F G LFA+T LV+IQ+ VGW++GYG+PT+G+A+++L+F+VGTPFYRH+
Sbjct: 797  KLSFFNWWFFSIFFGTLFANTFLVFIQERVGWTIGYGLPTLGLAVSVLVFLVGTPFYRHK 856

Query: 854  LPNGSPFTSMANVIVAAALNWRLPLPNHPNQLHELDLHHYSKPGTFKIDSTPSLRFLNKA 913
            LP+GSP T M  V VAA   W+L +P+ P +LHEL +  Y+  G  +ID + SL FL+KA
Sbjct: 857  LPSGSPITRMLQVYVAAVKKWKLRVPDDPKELHELSIEQYASGGRNRIDRSSSLSFLDKA 916

Query: 914  AIRRDSSDPWRLCTVTEVEETKQMLRMIPILICTFIPNTIMAQTHTLFIKQGTTLNRSIG 973
            +I+   + PWRLCTVT+VEETKQM ++IP+L+ T IP+T++ Q  TLF+KQGTTL+R +G
Sbjct: 917  SIKNGQTSPWRLCTVTQVEETKQMTKLIPVLLTTIIPSTLIVQASTLFVKQGTTLDRRMG 976

Query: 974  SHFKIPPASLNVFVTISMLLSILIYDRIFV-KMQRVTKNPRGITMLQRMGIGMICLVLVM 1033
             HF IPPA LN FVTI+ML+++++YDR+FV  ++R TKNPRGITMLQR+GIG++   ++M
Sbjct: 977  PHFHIPPACLNAFVTIAMLITVVLYDRVFVPAIRRYTKNPRGITMLQRLGIGLVLHCIIM 1036

Query: 1034 TVASRVEKHRLKIVAATENGSSAQVLPLTIFILLPQFILTGFAEAFVQVAVMEFFYDQAP 1093
             +A  +E+ RL++       S+   +PLTIFILLPQ+ L G A+ FV+VA ME FYDQAP
Sbjct: 1037 VIACFIERKRLRVARENHLFSAKDTIPLTIFILLPQYALGGVADNFVEVAKMELFYDQAP 1096

Query: 1094 ENMKSLGTSYTMTSLGIGNFLSSLILSKVSEITKRQGK-GWILNNLNASHLDYFYALLAV 1153
            + MKSL TSY  T+LGIG+FLSS +LS V++ITKR G  GWIL+NLN S LDY+YA +AV
Sbjct: 1097 DGMKSLATSYFTTTLGIGSFLSSFLLSTVADITKRNGHGGWILDNLNISRLDYYYAFMAV 1156

Query: 1154 MSAVNFFLFLLISKLYVYKAEVSDS 1165
            +S +N   FL+++K +VY  +V+ +
Sbjct: 1157 LSFLNLLCFLVVAKFFVYNVDVTQT 1176

BLAST of HG10012827.1 vs. NCBI nr
Match: KAF4364826.1 (hypothetical protein G4B88_025545 [Cannabis sativa])

HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 698/1224 (57.03%), Postives = 889/1224 (72.63%), Query Frame = 0

Query: 6    ADQETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLT 65
            A+QE G+DDYT+DGTVD KGNP+LRSK G WKACSF++VYE+ ERM + GI +NLIIYLT
Sbjct: 2    ANQEEGIDDYTEDGTVDLKGNPILRSKRGGWKACSFVVVYEVFERMAYYGIQSNLIIYLT 61

Query: 66   IKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAV 125
             KL+QGT+TASNNVTNW G +W+TPILGAY+ADA+LGRY TF ISS +    M +LTL+V
Sbjct: 62   EKLHQGTVTASNNVTNWIGAIWLTPILGAYIADAHLGRYPTFIISSSIYFTGMVILTLSV 121

Query: 126  SVPSLKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFD 185
            S+PSLKPPPCL+  N  NCK+AS LQLAVF+G+LY LA+ +GGTKPNIST+GADQFDDF 
Sbjct: 122  SIPSLKPPPCLDP-NLNNCKKASTLQLAVFYGALYTLALGTGGTKPNISTIGADQFDDFH 181

Query: 186  PKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGT 245
            PKEK QKLSFFNWW+FS+F G  FA+T+LV++QDN+GW+LGY +PT+GL ++I IF+ GT
Sbjct: 182  PKEKKQKLSFFNWWMFSIFFGTFFANTVLVWLQDNIGWTLGYALPTLGLAISIGIFLSGT 241

Query: 246  PFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSL 305
            PFYRH++P GSPF++MA VI+AA   W++P+P+D N+LYELDL+ Y K G ++ID TPS 
Sbjct: 242  PFYRHKVPTGSPFVKMAQVIIAAMRKWKVPIPHDLNELYELDLEVYEKKGKYRIDPTPS- 301

Query: 306  RFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTT 365
                                                                        
Sbjct: 302  ------------------------------------------------------------ 361

Query: 366  LDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMI 425
                                     LS+++YD  FVKI+QK TKNPRGIT+LQRMGIGMI
Sbjct: 362  -------------------------LSVVLYDWYFVKIIQKWTKNPRGITLLQRMGIGMI 421

Query: 426  CHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASN 485
             HI++M+ AS +E+HRL++A E G  + +    +PL+IFILLPQF+L G+ADAFL++A  
Sbjct: 422  FHIILMSTASLIERHRLSVAREYG--VVENGGQVPLSIFILLPQFVLMGIADAFLEVAKI 481

Query: 486  EFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG-KGWILNNLNVSHLD 545
            EFFYDQAPENMKSLG+SY MT+LG+G+FLS+F+LS VS ITKR G  GWILNNLN SHLD
Sbjct: 482  EFFYDQAPENMKSLGTSYAMTTLGVGSFLSSFLLSTVSNITKRNGHHGWILNNLNDSHLD 541

Query: 546  YFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSI--KLLTDELKKKKSK----------- 605
            Y+YA  A++S VNF  FL ISK YVYKAEVSDSI  + + +E+  K  +           
Sbjct: 542  YYYAFFAMLSFVNFICFLFISKYYVYKAEVSDSIHARCVLEEVNVKFQEFVNAEMAEVIT 601

Query: 606  ------GLQQT--------------------------------DQESMVDDYTKDGTVDL 665
                  GL ++                                ++E  +DDYT+DG+VDL
Sbjct: 602  HPISYLGLGESGAPFNSNFLVMDRAFKEGWCGMAVLARDYIMGNEEEGIDDYTEDGSVDL 661

Query: 666  KGKPLLRSKTGAWKACSFIIVYELMEKIMFHGIAANLIIYLTTKLHQGTVTASNNVTNWS 725
            KG P+ RSK G W+AC+F++VYE+ E++ ++GI +NLIIYL+ KLHQGTVTASNNVTNW 
Sbjct: 662  KGNPVRRSKRGGWRACAFVVVYEVFERMAYYGIQSNLIIYLSKKLHQGTVTASNNVTNWV 721

Query: 726  GTVWIMPILGAYIADAHLGRYRTFLISSFIWFTAMSLLTLAVSVPSLKPPPCLEAITKQN 785
            GTV + P+LGAYIADAHLGRY TF+I+S I+   M +LTL+VS+P LKPP CL++    N
Sbjct: 722  GTVTLTPVLGAYIADAHLGRYWTFIIASIIYLGGMFMLTLSVSIPMLKPPTCLDS-NPNN 781

Query: 786  CKQASKLQLAVFFGSLYLLALASGGTKPNISTMGADQFDDFDPKEKAQKLSFFNWWLFSV 845
            CK+ S LQ+AVFFG+LY LAL +GGTKPNIST+GADQFDDF+PKEK QK+SFFNWW+FS+
Sbjct: 782  CKKPSTLQVAVFFGALYTLALGTGGTKPNISTIGADQFDDFEPKEKKQKISFFNWWMFSI 841

Query: 846  FSGFLFASTVLVYIQDNVGWSLGYGIPTIGIAIAILIFVVGTPFYRHRLPNGSPFTSMAN 905
            F G  FA+TVLV++QDN+GW+LGY +PT+G+AI+I IF+ GTPFYRH++P GSPF  MA 
Sbjct: 842  FFGIFFANTVLVWLQDNIGWTLGYALPTLGLAISIGIFLAGTPFYRHKMPTGSPFVEMAQ 901

Query: 906  VIVAAALNWRLPLPNHPNQLHELDLHHYSKPGTFKIDSTPSLRFLNKAAIRRDSS---DP 965
            VI  A  N + PLP+ PN L+ELD   Y K G ++I  TP+LRFL+KA+++  SS    P
Sbjct: 902  VIFVAMRNRKAPLPHDPNDLYELDSQVYEKKGVYRIYPTPTLRFLSKASVKTGSSSTTSP 961

Query: 966  WRLCTVTEVEETKQMLRMIPILICTFIPNTIMAQTHTLFIKQGTTLNRSIGSHFKIPPAS 1025
            W+LC+VT+VEETKQM+RM+PI + TF+P+ ++AQ +TLFIKQGTTL R IG +F+IPPAS
Sbjct: 962  WKLCSVTKVEETKQMVRMLPIWVATFVPSIVLAQINTLFIKQGTTLQRGIG-NFEIPPAS 1021

Query: 1026 LNVFVTISMLLSILIYDRIFVK-MQRVTKNPRGITMLQRMGIGMICLVLVMTVASRVEKH 1085
            L+ FVT++ML+S+++YD  FVK +Q+ TKNPRGIT+LQRMGIGMI  ++VM VA   E+H
Sbjct: 1022 LSAFVTLTMLISVVLYDWYFVKIIQKWTKNPRGITLLQRMGIGMIFHIIVMFVAFLTERH 1081

Query: 1086 RLKIVAATENGSSAQVLPLTIFILLPQFILTGFAEAFVQVAVMEFFYDQAPENMKSLGTS 1145
            RL +        S   +PL+IFILLPQFI  G A+AF++VA ++FFYDQAPENMKSLG+S
Sbjct: 1082 RLSVAKEQGLVKSGGQVPLSIFILLPQFIFMGIADAFMEVAKIDFFYDQAPENMKSLGSS 1134

Query: 1146 YTMTSLGIGNFLSSLILSKVSEITKRQGKGWILNNLNASHLDYFYALLAVMSAVNFFLFL 1174
            Y MT++ +G FLSS +LS VS ITKR G GWILNNLN SHLDY+YA LA++S VN   FL
Sbjct: 1142 YNMTTVAVGGFLSSFLLSTVSNITKRNGHGWILNNLNDSHLDYYYAFLALLSFVNLICFL 1134

BLAST of HG10012827.1 vs. ExPASy Swiss-Prot
Match: Q9FNL7 (Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 3.9e-213
Identity = 363/580 (62.59%), Postives = 463/580 (79.83%), Query Frame = 0

Query: 8   QETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIK 67
           +E G DDYTKDGTVD +GNPV RS  G WKACSF++VYE+ ERM + GI++NL IY+T K
Sbjct: 4   EEVG-DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMTTK 63

Query: 68  LNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSV 127
           L+QGT+ +SNNVTNW GT W+TPILGAYV DA LGRY TF IS  +    M +LTL+V++
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSVTI 123

Query: 128 PSLKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPK 187
           P +KPP C    N ENC++AS LQLAVFFG+LY LAI +GGTKPNIST+GADQFD FDPK
Sbjct: 124 PGIKPPEC-STTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFDPK 183

Query: 188 EKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPF 247
           EK QKLSFFNWW+FS+F G LFA+T+LVY+QDNVGW+LGYG+PT+GL ++I IF++GTPF
Sbjct: 184 EKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGTPF 243

Query: 248 YRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRF 307
           YRH+LP GSPF +MA VIVA+      P+ +D    +EL    Y + G+F I  TPSLRF
Sbjct: 244 YRHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSLRF 303

Query: 308 LNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLD 367
           L++A+++ G++  W LCT TEVEETKQM+RM+P++  TF+PS M+AQ +TLF+KQGTTLD
Sbjct: 304 LDRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQGTTLD 363

Query: 368 RSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICH 427
           R +   F +PPASL  FVT+SML+SI++YDR+FVKI +K T NPRGIT+LQRMGIG+I H
Sbjct: 364 RKVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIGLIFH 423

Query: 428 ILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEF 487
           IL+M VAS  E++RL +AA++G  + Q    LPLTIF LLPQF+L G+AD+FL++A  EF
Sbjct: 424 ILIMIVASVTERYRLKVAADHG-LIHQTGVKLPLTIFALLPQFVLMGMADSFLEVAKLEF 483

Query: 488 FYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFY 547
           FYDQAPE+MKSLG+SY  TSL IGNF+S+F+LS VSEITK++G+GWILNNLN S LDY+Y
Sbjct: 484 FYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESRLDYYY 543

Query: 548 ALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKK 588
              AV++ VNF LFL++ K YVY+AEV+DS+ +   E+K+
Sbjct: 544 LFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of HG10012827.1 vs. ExPASy Swiss-Prot
Match: Q9FNL8 (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 1.3e-205
Identity = 354/600 (59.00%), Postives = 466/600 (77.67%), Query Frame = 0

Query: 8   QETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIK 67
           +E G DDYTKDGTVD +GN V RS+TG WKACSF++VYE+ ERM + GI++NL+IY+T K
Sbjct: 4   EEVG-DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTTK 63

Query: 68  LNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSV 127
           L+QGT+ +SNNVTNW GT W+TPILGAYVADA+ GRY TF ISS + L+ M+LLTL+VS+
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVSL 123

Query: 128 PSLKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPK 187
           P LKPP C  A N ENC++AS +QLAVFFG+LY LAI +GGTKPNIST+GADQFD+FDPK
Sbjct: 124 PGLKPPKCSTA-NVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPK 183

Query: 188 EKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPF 247
           +K  K SFFNWW+FS+F G  FA+T+LVY+QDNVGW++GYG+ T+GL  +I IF++GT  
Sbjct: 184 DKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRL 243

Query: 248 YRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRF 307
           YRH+LP GSPF +MA VIVA+    R P+ +D  + YEL    Y+   +F I ST SLRF
Sbjct: 244 YRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSLRF 303

Query: 308 LNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLD 367
           LN+A+++ GS+  WRLCT+TEVEETKQM++M+P++  TF+PS M+AQ  TLFIKQGTTLD
Sbjct: 304 LNRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQGTTLD 363

Query: 368 RSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICH 427
           R + ++F +PPASL  F T SML+SI+IYDR+FVK M+K+T NPRGIT+LQRMGIGMI H
Sbjct: 364 RRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIGMILH 423

Query: 428 ILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEF 487
           IL+M +AS  E++RL +AAE+G +  Q    +PL+IF LLPQ++L G+ADAF++IA  EF
Sbjct: 424 ILIMIIASITERYRLKVAAEHGLT-HQTAVPIPLSIFTLLPQYVLMGLADAFIEIAKLEF 483

Query: 488 FYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFY 547
           FYDQAPE+MKSLG+SY  TS+ +G F+S+ +LS VS+ITK+QG+GWI NNLN S LD +Y
Sbjct: 484 FYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESRLDNYY 543

Query: 548 ALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSKGLQQTDQESMVDDYTK 607
              AV++ +NF LFL++ + Y Y+A+V+ S  +              +  + +MVD+Y +
Sbjct: 544 MFFAVLNLLNFILFLVVIRFYEYRADVTQSANV--------------EQKEPNMVDNYNE 586

BLAST of HG10012827.1 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 568.2 bits (1463), Expect = 2.2e-160
Identity = 293/561 (52.23%), Postives = 404/561 (72.01%), Query Frame = 0

Query: 15  YTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGTLT 74
           YT+DGTVD +G PVL SKTG W+ACSF++ YE  ERM F GIA+NL+ YLT +L++ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 75  ASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPSLKPPP 134
           +  NV NW+G VWITPI GAY+AD+Y+GR+ TF  SSL+ ++ M LLT+AV+V SL+ P 
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLR-PT 126

Query: 135 CLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKLS 194
           C   +    C +AS LQ+  F+ SLY +AI +GGTKPNIST GADQFD +  +EK QK+S
Sbjct: 127 CENGV----CNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVS 186

Query: 195 FFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLPN 254
           FFNWW+FS F G LFA+  LVYIQ+N+GW LGYGIPT+GL V++++F +GTPFYRH++  
Sbjct: 187 FFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIK 246

Query: 255 GSPFIR-MANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAAI 314
                + +  V +AA  N +L  P+D  +LYELD  +Y  NG  ++  TP  RFL+KAAI
Sbjct: 247 TDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAI 306

Query: 315 RRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLDRSIGSH 374
           +  S  P   CTVT+VE  K+++ +I I + T IPST+ AQ +TLF+KQGTTLDR IGS+
Sbjct: 307 KTSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGSN 366

Query: 375 FKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICHILVMTV 434
           F++P ASL +FVT+SMLLS+ +YD+ FV  M+K T NPRGIT+LQR+G+G    I+ + +
Sbjct: 367 FQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIAI 426

Query: 435 ASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEFFYDQAP 494
           AS VE  R+ +  E    ++   +V+P++IF LLPQ+ L G+ D F  I   EFFYDQ+P
Sbjct: 427 ASAVEVKRMRVIKE--FHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQSP 486

Query: 495 ENMKSLGSSYFMTSLGIGNFLSTFILSKVSEIT-KRQGKGWILNNLNVSHLDYFYALLAV 554
           E M+SLG+++F + +G+GNFL++F+++ + +IT K  GK WI NNLN S LDY+Y  L V
Sbjct: 487 EEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFLVV 546

Query: 555 MSAVNFFLFLLISKLYVYKAE 574
           +S VN  LF+  +  YVYK++
Sbjct: 547 ISIVNMGLFVWAASKYVYKSD 557

BLAST of HG10012827.1 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.3e-136
Identity = 258/570 (45.26%), Postives = 373/570 (65.44%), Query Frame = 0

Query: 13  DDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGT 72
           D YT+DGTVD   NP  + KTG+WKAC FI+  E  ER+ + G+  NL+ YL  +LNQG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 73  LTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPSLKP 132
            TA+NNVTNW+GT +ITP++GA++ADAYLGRY T      + +  M+LLTL+ SVP LKP
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 133 PPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQK 192
             C    N + C   S  Q AVFF +LY++A+ +GG KP +S+ GADQFD+ D  EK +K
Sbjct: 125 GNC----NADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKK 184

Query: 193 LSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRL 252
            SFFNW+ FS+  G L A+T+LV+IQ NVGW  G+G+PT+ + +A+  F  G+ FYR + 
Sbjct: 185 SSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQR 244

Query: 253 PNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAA 312
           P GSP  R+  VIVAA     + +P D + L+E      +  GS K+  T +L+F +KAA
Sbjct: 245 PGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAA 304

Query: 313 -------IRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTT 372
                  I+ G  +PWRLC+VT+VEE K ++ ++P+     + +T+ +Q  T+F+ QG T
Sbjct: 305 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 364

Query: 373 LDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMI 432
           +D+ +G +F++P ASL  F T+S+L    +YD+  + + +K T+N RG T LQRMGIG++
Sbjct: 365 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 424

Query: 433 CHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASN 492
             I  M  A  +E  RL+    +    + +QK + ++IF  +PQ++L G A+ F  I   
Sbjct: 425 VSIFAMITAGVLEVVRLDYVKTHN---AYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQL 484

Query: 493 EFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGK-GWILNNLNVSHLD 552
           EFFYDQAP+ M+SL S+  +T++ +GN+LST +++ V +ITK+ GK GWI +NLN  HLD
Sbjct: 485 EFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLD 544

Query: 553 YFYALLAVMSAVNFFLFLLISKLYVYKAEV 575
           YF+ LLA +S +NF ++L ISK Y YK  V
Sbjct: 545 YFFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of HG10012827.1 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.6e-129
Identity = 253/565 (44.78%), Postives = 366/565 (64.78%), Query Frame = 0

Query: 15  YTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGTLT 74
           Y +DG+VD  GNP L+ KTG+WKAC FI+  E  ER+ + GIA NLI YLT KL+QG ++
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 75  ASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPSLKPPP 134
           A+ NVT W GT ++TP++GA +ADAY GRY T    S +  + MS LTL+ SVP+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 135 CLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKLS 194
           C+     + C  A+  Q A+FFG LYL+A+ +GG KP +S+ GADQFDD D +E+ +K S
Sbjct: 144 CI----GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKAS 203

Query: 195 FFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLPN 254
           FFNW+ FS+  G L +S++LV+IQ+N GW LG+GIPT+ +G+AI  F  GTP YR + P 
Sbjct: 204 FFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPG 263

Query: 255 GSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAAI- 314
           GSP  R++ V+VA+     + +P D   LYE   ++ +  GS KI+ T   ++L+KAA+ 
Sbjct: 264 GSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVI 323

Query: 315 -----RRGS-SDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLD 374
                + G  S+ WRLCTVT+VEE K ++RM PI     I S + AQ  T+F++QG  ++
Sbjct: 324 SEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMN 383

Query: 375 RSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICH 434
             IGS F++PPA+L  F T S+++ + +YDR  V + +K T   +G T +QRMGIG+   
Sbjct: 384 CKIGS-FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVS 443

Query: 435 ILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEF 494
           +L M  A+ VE  RL++A  N   L +    +P+++   +PQ+ + G A+ F  I   EF
Sbjct: 444 VLCMAAAAIVEIIRLHMA--NDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEF 503

Query: 495 FYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG-KGWILNNLNVSHLDYF 554
           FYDQ+P+ M+SL S+  + +  +GN+LS+ IL+ V+  T R G +GWI +NLN  HLDYF
Sbjct: 504 FYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYF 563

Query: 555 YALLAVMSAVNFFLFLLISKLYVYK 572
           + LLA +S VN  ++   +  Y  K
Sbjct: 564 FWLLAGLSLVNMAVYFFSAARYKQK 581

BLAST of HG10012827.1 vs. ExPASy TrEMBL
Match: A0A445CPT7 (Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_A06g027793 PE=3 SV=1)

HSP 1 Score: 1378.2 bits (3566), Expect = 0.0e+00
Identity = 679/1193 (56.92%), Postives = 912/1193 (76.45%), Query Frame = 0

Query: 3    GAAADQETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLII 62
            G    +E G +DYT+DGTVD KG PVLRS TG WKACSFI+ YE++ERM + GIA+NL++
Sbjct: 2    GVLLSEEKG-EDYTEDGTVDLKGRPVLRSNTGKWKACSFIVGYEMVERMAYYGIASNLVV 61

Query: 63   YLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLT 122
            YLT +L++GT+ +S NVTNW G VW  P +GAY+ADAYLGRY TF ISS + L+ M LLT
Sbjct: 62   YLTKELHEGTVKSSKNVTNWVGVVWFMPAIGAYIADAYLGRYSTFLISSAIYLLGMCLLT 121

Query: 123  LAVSVPSLKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFD 182
            LAVS+P+LKPPPC +    ++C++A+ LQ+ +FF  LY++A+ +GGTKPNISTMGADQFD
Sbjct: 122  LAVSLPALKPPPCPQ---DKDCQKATSLQVGLFFLGLYIIAVGTGGTKPNISTMGADQFD 181

Query: 183  DFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFV 242
             F+PKEKAQK+SFFNWW+  +  G +F++T+LVYIQDNVGW+LGYGIPT GL  +IL+F+
Sbjct: 182  KFEPKEKAQKISFFNWWVTFILIGTIFSNTVLVYIQDNVGWALGYGIPTGGLLFSILVFL 241

Query: 243  VGTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDST 302
             GTPFYRH+ P+GSP  RM  VIVAA   W+L +P+DP +LYEL ++ Y+ NG  +I  +
Sbjct: 242  FGTPFYRHKSPSGSPLTRMLQVIVAAVRKWKLEVPDDPKELYELTVEEYAINGRNRIYHS 301

Query: 303  PSLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQ 362
            PSL FL+KAAI+   + PW LCT+T+VEETKQM++M+PIM+ T +PST++AQ++TLFIKQ
Sbjct: 302  PSLSFLDKAAIKTKQTQPWMLCTMTQVEETKQMMKMVPIMVTTCMPSTVIAQANTLFIKQ 361

Query: 363  GTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGI 422
            GTTLDRSIG +FK+PPA L AF+ I MLLS++ YDR+ V ++++ TKNPRGIT+LQR+GI
Sbjct: 362  GTTLDRSIGPNFKIPPACLTAFINIFMLLSVVTYDRVLVPLVRRYTKNPRGITLLQRLGI 421

Query: 423  GMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQI 482
            G++ HI++M  A   EK RL++A ++  +L  +  +LPL+IFILLPQF L G+AD F+ +
Sbjct: 422  GLVIHIVIMITACLAEKKRLSVARQH--NLLGQHDILPLSIFILLPQFALAGIADTFVDV 481

Query: 483  ASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG--KGWILNNLNV 542
            A  + FYDQAPE MKSLG+SY   SL IG F S+F++S V+++TKR    KGWIL+NLNV
Sbjct: 482  AKLDLFYDQAPEGMKSLGTSYVFISLSIGTFFSSFLISTVADLTKRNNGQKGWILDNLNV 541

Query: 543  SHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDS----IKLLTDELKKKKSKGLQQT 602
            SHLDY++A LA++SA+NF  FL+ +K +VY  + + +    +++  +         L Q 
Sbjct: 542  SHLDYYFAFLAILSAINFLCFLVAAKFFVYNNDATQASIIGLEMKNNNASSHDKMELNQK 601

Query: 603  DQ--ESMVDDYTKDGTVDLKGKPLLRSKTGAWKACSFIIVYELMEKIMFHGIAANLIIYL 662
            ++    + +DYT+DGTVDLKG+P+LRSKTG WKACSFI+ YE+ E++ ++GIA+NL+ YL
Sbjct: 602  EKAPARLEEDYTQDGTVDLKGRPVLRSKTGKWKACSFIVGYEVFERMAYYGIASNLVQYL 661

Query: 663  TTKLHQGTVTASNNVTNWSGTVWIMPILGAYIADAHLGRYRTFLISSFIWFTAMSLLTLA 722
            T KLH+G V +SNNV+NW G+VW+ P+ GAYIADA+LGRY TFLISS I+   M L+TLA
Sbjct: 662  TEKLHEGIVNSSNNVSNWVGSVWMTPLAGAYIADAYLGRYWTFLISSAIYLLGMVLITLA 721

Query: 723  VSVPSLKPPPCLEAITKQNCKQASKLQLAVFFGSLYLLALASGGTKPNISTMGADQFDDF 782
            VSV +L+PPPC   +   NC +A+KLQL +FF +LY +A+ +GGTKPNISTMGADQFD+F
Sbjct: 722  VSVRALRPPPCPVGVDDANCPRATKLQLGIFFLALYTIAVGTGGTKPNISTMGADQFDEF 781

Query: 783  DPKEKAQKLSFFNWWLFSVFSGFLFASTVLVYIQDNVGWSLGYGIPTIGIAIAILIFVVG 842
            +PKE+  KLSFFNWW+FS+F G LF++T LVYIQ+ V W++GYG+PTIG+A++IL+F+ G
Sbjct: 782  EPKERHHKLSFFNWWMFSIFFGTLFSNTFLVYIQEKVSWTIGYGLPTIGLAVSILVFLFG 841

Query: 843  TPFYRHRLPNGSPFTSMANVIVAAALNWRLPLPNHPNQLHELDLHHYSKPGTFKIDSTPS 902
            TPFYRH+LP+GSP T +  V VAA   W++ +P  P +LHEL +  Y   G  +ID +PS
Sbjct: 842  TPFYRHKLPSGSPITRILQVYVAAFRKWKVHIPGDPKELHELSIEEYVSNGRTRIDHSPS 901

Query: 903  LRFLNKAAIRRDSSDPWRLCTVTEVEETKQMLRMIPILICTFIPNTIMAQTHTLFIKQGT 962
              FL+KAA R D + PW LCTVT+VEETKQM +M+PILI T +P+T++ Q  TLFIKQG 
Sbjct: 902  FSFLDKAATRTDQTSPWMLCTVTQVEETKQMTKMVPILITTLLPSTMLIQATTLFIKQGN 961

Query: 963  TLNRSIGSHFKIPPASLNVFVTISMLLSILIYDRIFVK-MQRVTKNPRGITMLQRMGIGM 1022
            TLNRS+G  F IPPA L  F+TI ML+SI+IYDR+FV  ++R TKNPRGIT+LQR+GIG+
Sbjct: 962  TLNRSMGPDFDIPPACLTSFITIFMLISIVIYDRVFVPVIRRYTKNPRGITLLQRLGIGL 1021

Query: 1023 ICLVLVMTVASRVEKHRLKIVAATENGSSAQVLPLTIFILLPQFILTGFAEAFVQVAVME 1082
            +  ++V+  AS VE+ RL +            LPLTIFILLPQF LTG A+ FV+VA +E
Sbjct: 1022 VIHIIVLITASFVERKRLSVAREHNLLRQHDQLPLTIFILLPQFALTGIADNFVEVAKLE 1081

Query: 1083 FFYDQAPENMKSLGTSYTMTSLGIGNFLSSLILSKVSEITKRQG-KGWILNNLNASHLDY 1142
            FFYDQAPE MKS+GTSY  TSLGIG+FL++ +L+ V+ +TKR G KGW+LNNLN SHLDY
Sbjct: 1082 FFYDQAPEGMKSMGTSYFTTSLGIGSFLATFLLTTVANLTKRNGHKGWVLNNLNVSHLDY 1141

Query: 1143 FYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIRLLTDELKKKKSKASSNSQV 1186
            +YA +A +S +N   FL+++K +VY  +V+     L  E+    S+  SN+++
Sbjct: 1142 YYAFMAGLSFINLLCFLVVAKFFVYNDDVAQKKTGL--EMNTASSQGYSNNRI 1186

BLAST of HG10012827.1 vs. ExPASy TrEMBL
Match: A0A371FPY9 (Protein NRT1/ PTR FAMILY 5.2 (Fragment) OS=Mucuna pruriens OX=157652 GN=NPF5.2 PE=3 SV=1)

HSP 1 Score: 1374.0 bits (3555), Expect = 0.0e+00
Identity = 677/1202 (56.32%), Postives = 911/1202 (75.79%), Query Frame = 0

Query: 10   TGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLN 69
            +G +DYT+DGTVD KG PVLRS TG W+ACSFI+ YE+IERM + GIA+NL++YLT KL+
Sbjct: 11   SGREDYTQDGTVDLKGRPVLRSNTGRWRACSFIVGYEMIERMAYYGIASNLVLYLTKKLH 70

Query: 70   QGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPS 129
            +GT+ +SN+VTNW GTVW+ P  GAY+ADAYLGRY TF I+S + L+ M LLTL VS+P+
Sbjct: 71   EGTVKSSNHVTNWVGTVWMMPAAGAYIADAYLGRYSTFVIASAIYLLGMCLLTLTVSLPA 130

Query: 130  LKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEK 189
            LKPPPC   +  ++C++AS LQ+ +FF +LY++A  +GGTKPNISTMGADQFD+F+P+E+
Sbjct: 131  LKPPPCALGVADKDCQRASSLQVGIFFCALYIIAAGTGGTKPNISTMGADQFDEFEPRER 190

Query: 190  AQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYR 249
            +QKLSF+NWW+F++  G +FA T+LVYIQD VG+ LGYGIPTIGL ++IL+F++GTP YR
Sbjct: 191  SQKLSFYNWWVFNILIGTIFAQTLLVYIQDKVGFGLGYGIPTIGLALSILVFLLGTPLYR 250

Query: 250  HRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDL-QHYSKNGSFKIDSTPSLR-- 309
            HRLP+GSP  RM  V VAA   W++ +P+D N+L+EL + ++Y+  G  +I  + SLR  
Sbjct: 251  HRLPSGSPLTRMVQVFVAAMTKWKVHVPDDVNELHELSIEEYYASKGRSRIYHSSSLRLH 310

Query: 310  -----------FLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQS 369
                       FL+KAA++ G +  W LCTVT+VEETKQM++MIPI+I T +PST++AQ+
Sbjct: 311  NNLITLTNVSSFLDKAAVKTGQTSQWMLCTVTQVEETKQMMKMIPILITTCVPSTIIAQT 370

Query: 370  HTLFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGIT 429
             TLFI+QGTTLDR +G HF++PPA L AFV I ML+S++IYDR FV  +++ TK+PRGI+
Sbjct: 371  STLFIRQGTTLDRRMGPHFQIPPACLIAFVNIFMLISVVIYDRFFVPSIRRYTKDPRGIS 430

Query: 430  MLQRMGIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGV 489
            +LQR+GIG++ H+++M  A  VE+ RL +A EN   L ++   +PLTIFILLPQF LTG+
Sbjct: 431  LLQRLGIGLVLHVIIMLTACLVERKRLGVAREN--HLLEQNDTIPLTIFILLPQFALTGI 490

Query: 490  ADAFLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG-KGWI 549
            AD F+ +A  EFFYDQAPE+MKSLG+SYF T+L IGNFLSTF+LS V+++T+R G KGWI
Sbjct: 491  ADTFVDVAKLEFFYDQAPESMKSLGTSYFTTTLSIGNFLSTFLLSTVADLTRRNGHKGWI 550

Query: 550  LNNLNVSHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDS-IKLLTDELKKKKSKGL 609
            L+NLNVS LDY+YA LA++SA+N   F++++KLYVY  +V+ + + L  +    K + G+
Sbjct: 551  LDNLNVSRLDYYYAFLAMLSAINLLCFVVVAKLYVYNVDVTQTKMDLDMNPASSKDNNGI 610

Query: 610  QQTDQE---------------SMVDDYTKDGTVDLKGKPLLRSKTGAWKACSFII----- 669
             Q+  +               S  +DYT+DGTVDL G+PLLRSKTG WKACSFI+     
Sbjct: 611  SQSTPQPDAKLMAVVEEKGPASGNEDYTQDGTVDLMGRPLLRSKTGRWKACSFIVGYEYG 670

Query: 670  ---------VYELMEKIMFHGIAANLIIYLTTKLHQGTVTASNNVTNWSGTVWIMPILGA 729
                      YE+ E++ F+GI +NL++YLT KLH+GTV +SN+V+NW G+VW+MP+ GA
Sbjct: 671  YACVCTKSTGYEVFERMAFYGIQSNLVLYLTKKLHEGTVKSSNHVSNWVGSVWMMPLAGA 730

Query: 730  YIADAHLGRYRTFLISSFIWFTAMSLLTLAVSVPSLKPPPCLEAITKQNCKQASKLQLAV 789
            YIADA+LGRY TF+I+S I+   M LLTLAVS+P L+PPPC +    +NC +AS LQ  +
Sbjct: 731  YIADAYLGRYWTFVIASCIYVLGMCLLTLAVSLPVLRPPPCAQ---DENCPEASSLQYGI 790

Query: 790  FFGSLYLLALASGGTKPNISTMGADQFDDFDPKEKAQKLSFFNWWLFSVFSGFLFASTVL 849
            FF +LY +A+ +GGTKPNISTMGADQFD+F+PKE++ KLSFFNWW FS+F G LFA+T L
Sbjct: 791  FFTALYTIAIGTGGTKPNISTMGADQFDEFEPKERSHKLSFFNWWFFSIFFGTLFANTFL 850

Query: 850  VYIQDNVGWSLGYGIPTIGIAIAILIFVVGTPFYRHRLPNGSPFTSMANVIVAAALNWRL 909
            VYIQD VGW++GYG+PT+G+A+++L+F+VGTP+YRHRLP+GSP T +  V VAA   W+L
Sbjct: 851  VYIQDRVGWTIGYGLPTLGLAVSVLLFLVGTPYYRHRLPSGSPITRVLQVFVAAGRKWKL 910

Query: 910  PLPNHPNQLHELDLHHYSKPGTFKIDSTPSLRFLNKAAIRRDSSDPWRLCTVTEVEETKQ 969
             +P+ P +LHEL +  Y+  G  +ID + SL FLNKAAI+   +  W L TVT+VEETKQ
Sbjct: 911  KVPDDPKELHELSIEEYASSGRSRIDHSSSLSFLNKAAIKSGQTSAWMLSTVTQVEETKQ 970

Query: 970  MLRMIPILICTFIPNTIMAQTHTLFIKQGTTLNRSIGSHFKIPPASLNVFVTISMLLSIL 1029
            M +++PIL+ T IP+T+  QT T+F+KQG TL+R +G HF IPPA L  FVTISML++I+
Sbjct: 971  MTKLMPILLTTIIPSTLYVQTSTIFVKQGATLDRRMGPHFDIPPACLTAFVTISMLITIV 1030

Query: 1030 IYDRIFVKM-QRVTKNPRGITMLQRMGIGMICLVLVMTVASRVEKHRLKIVAATENGSSA 1089
            IYDR+FV + +R TKNPRGITMLQR+GIG++  V VM  A   E+ RL++V         
Sbjct: 1031 IYDRVFVPLIRRYTKNPRGITMLQRLGIGLVLHVTVMITACLAERRRLRVVRENHLFGPH 1090

Query: 1090 QVLPLTIFILLPQFILTGFAEAFVQVAVMEFFYDQAPENMKSLGTSYTMTSLGIGNFLSS 1149
              +PLTIFILLPQ+ L G A+ FV+VA ME FYDQAP  MKSLGT+Y  TSLG+G+FLSS
Sbjct: 1091 DTIPLTIFILLPQYALAGVADNFVEVAKMELFYDQAPYGMKSLGTAYFTTSLGVGSFLSS 1150

Query: 1150 LILSKVSEITKRQG-KGWILNNLNASHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVS 1165
             +LS V+ ITKR G  GW+L+NLN SHLDY+YA +AV+S +N   FL+++K +VY  +V+
Sbjct: 1151 FLLSTVANITKRHGHTGWVLDNLNVSHLDYYYAFMAVLSLLNLLCFLVVAKFFVYNVDVT 1207

BLAST of HG10012827.1 vs. ExPASy TrEMBL
Match: A0A4D6M8Z0 (Solute carrier family 15 OS=Vigna unguiculata OX=3917 GN=DEO72_LG6g2477 PE=3 SV=1)

HSP 1 Score: 1368.2 bits (3540), Expect = 0.0e+00
Identity = 664/1165 (57.00%), Postives = 904/1165 (77.60%), Query Frame = 0

Query: 14   DYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGTL 73
            DYTKDGT+D KG PVLRS TG W+ACSFI+ YE+IERM + GIA+NL++YLT KL++GT+
Sbjct: 17   DYTKDGTLDLKGKPVLRSNTGRWRACSFIVGYEMIERMAYYGIASNLVLYLTKKLHEGTV 76

Query: 74   TASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPSLKPP 133
             +SN+VTNW G VWI P  GAY+ADA+LGRY TF ISS + L+ M LLTLAVS+P L+PP
Sbjct: 77   KSSNHVTNWAGAVWIMPAAGAYIADAFLGRYWTFVISSAIYLLGMCLLTLAVSLPGLRPP 136

Query: 134  PCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKL 193
             C   I  ++C QAS LQ+ +FF +LY++A  +GGTKPNISTMGADQFD+F+PKE++QKL
Sbjct: 137  ACAPGIADQDCPQASSLQVGIFFFALYIIAAGTGGTKPNISTMGADQFDEFEPKERSQKL 196

Query: 194  SFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLP 253
            SF+NWW+F++  G + A T+LVYIQD VG+ LGYGIPTI L V+I +F++GTP YRHRLP
Sbjct: 197  SFYNWWVFNILIGTISAQTLLVYIQDRVGFGLGYGIPTIALAVSIFMFLLGTPLYRHRLP 256

Query: 254  NGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQH-YSKNGSFKIDSTPS-------L 313
            +GSP  RM  V+++A   W++ +P+D N+L+EL ++  Y+  G  +I  T          
Sbjct: 257  SGSPLTRMLQVLLSAVRKWKVHVPHDLNELHELSVEECYASKGRTRIQHTQEYCNLTNVC 316

Query: 314  RFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTT 373
            RFL+KAA++ G + PW LCTVT++EE KQM++M+PI+I T IPST++AQ+ TLFI+QGTT
Sbjct: 317  RFLDKAAVKTGETSPWMLCTVTQIEEAKQMMKMVPILITTCIPSTIIAQTTTLFIRQGTT 376

Query: 374  LDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMI 433
            LDR IG HF++PPA L AFV I ML+S++IYDR+FV  ++  TKNPRGI++LQR+GIG++
Sbjct: 377  LDRRIGPHFEIPPACLIAFVNIFMLISVVIYDRLFVPAIRHYTKNPRGISLLQRLGIGLV 436

Query: 434  CHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASN 493
             H+++M  A  VE+ RL++A E  + L Q  K+ PLTIFILLPQF LTG+AD F+ +A  
Sbjct: 437  LHVIIMLTACFVERKRLSVAREK-NLLGQLDKI-PLTIFILLPQFALTGIADTFVDVAKL 496

Query: 494  EFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG-KGWILNNLNVSHLD 553
            EFFYDQAPE MKSLG+SYF T+L IGNFL++F+LS V+++T R G K WIL+NLN S LD
Sbjct: 497  EFFYDQAPEAMKSLGTSYFTTTLSIGNFLNSFLLSTVADLTHRHGHKSWILDNLNASRLD 556

Query: 554  YFYALLAVMSAVNFFLFLLISKLYVY---KAEVSDSIKLLTDELKKKKSKGLQQTDQESM 613
            Y+YA LA++SA+NFF F+ ++KLYVY   + +++  + +  D  +       ++    + 
Sbjct: 557  YYYAFLALLSAINFFCFVAVAKLYVYNGDETQINKDLDMNPDSPQDNTEISQKEKGPANG 616

Query: 614  VDDYTKDGTVDLKGKPLLRSKTGAWKACSFIIVYELMEKIMFHGIAANLIIYLTTKLHQG 673
             +DYT+DGTVDLKG+P+LR++TG WKACSFI+ YE+ E++ F+GI +NL+IYLT KLH+G
Sbjct: 617  NEDYTQDGTVDLKGRPVLRTETGKWKACSFIVGYEVFERMAFYGIQSNLVIYLTRKLHEG 676

Query: 674  TVTASNNVTNWSGTVWIMPILGAYIADAHLGRYRTFLISSFIWFTAMSLLTLAVSVPSLK 733
            TV +SN+V+NW G+VW+MP+ GAYIADA LGRY+TF+I+S I+   M LLTLAVS+P+L+
Sbjct: 677  TVKSSNDVSNWVGSVWMMPLAGAYIADAFLGRYKTFIIASCIYVAGMCLLTLAVSLPALR 736

Query: 734  PPPCLEAITKQNCKQASKLQLAVFFGSLYLLALASGGTKPNISTMGADQFDDFDPKEKAQ 793
            PP C E    +NC +AS LQ  +FF +LY++A+ +GGTKPNISTMGADQFD+F+PKE++ 
Sbjct: 737  PPQCDEG---ENCPEASSLQYGIFFLALYIIAIGTGGTKPNISTMGADQFDEFEPKERSY 796

Query: 794  KLSFFNWWLFSVFSGFLFASTVLVYIQDNVGWSLGYGIPTIGIAIAILIFVVGTPFYRHR 853
            KLSFFNWW FS+F G LFA+T LV+IQ+ VGW++GYG+PT+G+A+++L+F+VGTPFYRH+
Sbjct: 797  KLSFFNWWFFSIFFGTLFANTFLVFIQERVGWTIGYGLPTLGLAVSVLVFLVGTPFYRHK 856

Query: 854  LPNGSPFTSMANVIVAAALNWRLPLPNHPNQLHELDLHHYSKPGTFKIDSTPSLRFLNKA 913
            LP+GSP T M  V VAA   W+L +P+ P +LHEL +  Y+  G  +ID + SL FL+KA
Sbjct: 857  LPSGSPITRMLQVYVAAVKKWKLRVPDDPKELHELSIEQYASGGRNRIDRSSSLSFLDKA 916

Query: 914  AIRRDSSDPWRLCTVTEVEETKQMLRMIPILICTFIPNTIMAQTHTLFIKQGTTLNRSIG 973
            +I+   + PWRLCTVT+VEETKQM ++IP+L+ T IP+T++ Q  TLF+KQGTTL+R +G
Sbjct: 917  SIKNGQTSPWRLCTVTQVEETKQMTKLIPVLLTTIIPSTLIVQASTLFVKQGTTLDRRMG 976

Query: 974  SHFKIPPASLNVFVTISMLLSILIYDRIFV-KMQRVTKNPRGITMLQRMGIGMICLVLVM 1033
             HF IPPA LN FVTI+ML+++++YDR+FV  ++R TKNPRGITMLQR+GIG++   ++M
Sbjct: 977  PHFHIPPACLNAFVTIAMLITVVLYDRVFVPAIRRYTKNPRGITMLQRLGIGLVLHCIIM 1036

Query: 1034 TVASRVEKHRLKIVAATENGSSAQVLPLTIFILLPQFILTGFAEAFVQVAVMEFFYDQAP 1093
             +A  +E+ RL++       S+   +PLTIFILLPQ+ L G A+ FV+VA ME FYDQAP
Sbjct: 1037 VIACFIERKRLRVARENHLFSAKDTIPLTIFILLPQYALGGVADNFVEVAKMELFYDQAP 1096

Query: 1094 ENMKSLGTSYTMTSLGIGNFLSSLILSKVSEITKRQGK-GWILNNLNASHLDYFYALLAV 1153
            + MKSL TSY  T+LGIG+FLSS +LS V++ITKR G  GWIL+NLN S LDY+YA +AV
Sbjct: 1097 DGMKSLATSYFTTTLGIGSFLSSFLLSTVADITKRNGHGGWILDNLNISRLDYYYAFMAV 1156

Query: 1154 MSAVNFFLFLLISKLYVYKAEVSDS 1165
            +S +N   FL+++K +VY  +V+ +
Sbjct: 1157 LSFLNLLCFLVVAKFFVYNVDVTQT 1176

BLAST of HG10012827.1 vs. ExPASy TrEMBL
Match: A0A7J6F2G0 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_025545 PE=3 SV=1)

HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 698/1224 (57.03%), Postives = 889/1224 (72.63%), Query Frame = 0

Query: 6    ADQETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLT 65
            A+QE G+DDYT+DGTVD KGNP+LRSK G WKACSF++VYE+ ERM + GI +NLIIYLT
Sbjct: 2    ANQEEGIDDYTEDGTVDLKGNPILRSKRGGWKACSFVVVYEVFERMAYYGIQSNLIIYLT 61

Query: 66   IKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAV 125
             KL+QGT+TASNNVTNW G +W+TPILGAY+ADA+LGRY TF ISS +    M +LTL+V
Sbjct: 62   EKLHQGTVTASNNVTNWIGAIWLTPILGAYIADAHLGRYPTFIISSSIYFTGMVILTLSV 121

Query: 126  SVPSLKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFD 185
            S+PSLKPPPCL+  N  NCK+AS LQLAVF+G+LY LA+ +GGTKPNIST+GADQFDDF 
Sbjct: 122  SIPSLKPPPCLDP-NLNNCKKASTLQLAVFYGALYTLALGTGGTKPNISTIGADQFDDFH 181

Query: 186  PKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGT 245
            PKEK QKLSFFNWW+FS+F G  FA+T+LV++QDN+GW+LGY +PT+GL ++I IF+ GT
Sbjct: 182  PKEKKQKLSFFNWWMFSIFFGTFFANTVLVWLQDNIGWTLGYALPTLGLAISIGIFLSGT 241

Query: 246  PFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSL 305
            PFYRH++P GSPF++MA VI+AA   W++P+P+D N+LYELDL+ Y K G ++ID TPS 
Sbjct: 242  PFYRHKVPTGSPFVKMAQVIIAAMRKWKVPIPHDLNELYELDLEVYEKKGKYRIDPTPS- 301

Query: 306  RFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTT 365
                                                                        
Sbjct: 302  ------------------------------------------------------------ 361

Query: 366  LDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMI 425
                                     LS+++YD  FVKI+QK TKNPRGIT+LQRMGIGMI
Sbjct: 362  -------------------------LSVVLYDWYFVKIIQKWTKNPRGITLLQRMGIGMI 421

Query: 426  CHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASN 485
             HI++M+ AS +E+HRL++A E G  + +    +PL+IFILLPQF+L G+ADAFL++A  
Sbjct: 422  FHIILMSTASLIERHRLSVAREYG--VVENGGQVPLSIFILLPQFVLMGIADAFLEVAKI 481

Query: 486  EFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG-KGWILNNLNVSHLD 545
            EFFYDQAPENMKSLG+SY MT+LG+G+FLS+F+LS VS ITKR G  GWILNNLN SHLD
Sbjct: 482  EFFYDQAPENMKSLGTSYAMTTLGVGSFLSSFLLSTVSNITKRNGHHGWILNNLNDSHLD 541

Query: 546  YFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSI--KLLTDELKKKKSK----------- 605
            Y+YA  A++S VNF  FL ISK YVYKAEVSDSI  + + +E+  K  +           
Sbjct: 542  YYYAFFAMLSFVNFICFLFISKYYVYKAEVSDSIHARCVLEEVNVKFQEFVNAEMAEVIT 601

Query: 606  ------GLQQT--------------------------------DQESMVDDYTKDGTVDL 665
                  GL ++                                ++E  +DDYT+DG+VDL
Sbjct: 602  HPISYLGLGESGAPFNSNFLVMDRAFKEGWCGMAVLARDYIMGNEEEGIDDYTEDGSVDL 661

Query: 666  KGKPLLRSKTGAWKACSFIIVYELMEKIMFHGIAANLIIYLTTKLHQGTVTASNNVTNWS 725
            KG P+ RSK G W+AC+F++VYE+ E++ ++GI +NLIIYL+ KLHQGTVTASNNVTNW 
Sbjct: 662  KGNPVRRSKRGGWRACAFVVVYEVFERMAYYGIQSNLIIYLSKKLHQGTVTASNNVTNWV 721

Query: 726  GTVWIMPILGAYIADAHLGRYRTFLISSFIWFTAMSLLTLAVSVPSLKPPPCLEAITKQN 785
            GTV + P+LGAYIADAHLGRY TF+I+S I+   M +LTL+VS+P LKPP CL++    N
Sbjct: 722  GTVTLTPVLGAYIADAHLGRYWTFIIASIIYLGGMFMLTLSVSIPMLKPPTCLDS-NPNN 781

Query: 786  CKQASKLQLAVFFGSLYLLALASGGTKPNISTMGADQFDDFDPKEKAQKLSFFNWWLFSV 845
            CK+ S LQ+AVFFG+LY LAL +GGTKPNIST+GADQFDDF+PKEK QK+SFFNWW+FS+
Sbjct: 782  CKKPSTLQVAVFFGALYTLALGTGGTKPNISTIGADQFDDFEPKEKKQKISFFNWWMFSI 841

Query: 846  FSGFLFASTVLVYIQDNVGWSLGYGIPTIGIAIAILIFVVGTPFYRHRLPNGSPFTSMAN 905
            F G  FA+TVLV++QDN+GW+LGY +PT+G+AI+I IF+ GTPFYRH++P GSPF  MA 
Sbjct: 842  FFGIFFANTVLVWLQDNIGWTLGYALPTLGLAISIGIFLAGTPFYRHKMPTGSPFVEMAQ 901

Query: 906  VIVAAALNWRLPLPNHPNQLHELDLHHYSKPGTFKIDSTPSLRFLNKAAIRRDSS---DP 965
            VI  A  N + PLP+ PN L+ELD   Y K G ++I  TP+LRFL+KA+++  SS    P
Sbjct: 902  VIFVAMRNRKAPLPHDPNDLYELDSQVYEKKGVYRIYPTPTLRFLSKASVKTGSSSTTSP 961

Query: 966  WRLCTVTEVEETKQMLRMIPILICTFIPNTIMAQTHTLFIKQGTTLNRSIGSHFKIPPAS 1025
            W+LC+VT+VEETKQM+RM+PI + TF+P+ ++AQ +TLFIKQGTTL R IG +F+IPPAS
Sbjct: 962  WKLCSVTKVEETKQMVRMLPIWVATFVPSIVLAQINTLFIKQGTTLQRGIG-NFEIPPAS 1021

Query: 1026 LNVFVTISMLLSILIYDRIFVK-MQRVTKNPRGITMLQRMGIGMICLVLVMTVASRVEKH 1085
            L+ FVT++ML+S+++YD  FVK +Q+ TKNPRGIT+LQRMGIGMI  ++VM VA   E+H
Sbjct: 1022 LSAFVTLTMLISVVLYDWYFVKIIQKWTKNPRGITLLQRMGIGMIFHIIVMFVAFLTERH 1081

Query: 1086 RLKIVAATENGSSAQVLPLTIFILLPQFILTGFAEAFVQVAVMEFFYDQAPENMKSLGTS 1145
            RL +        S   +PL+IFILLPQFI  G A+AF++VA ++FFYDQAPENMKSLG+S
Sbjct: 1082 RLSVAKEQGLVKSGGQVPLSIFILLPQFIFMGIADAFMEVAKIDFFYDQAPENMKSLGSS 1134

Query: 1146 YTMTSLGIGNFLSSLILSKVSEITKRQGKGWILNNLNASHLDYFYALLAVMSAVNFFLFL 1174
            Y MT++ +G FLSS +LS VS ITKR G GWILNNLN SHLDY+YA LA++S VN   FL
Sbjct: 1142 YNMTTVAVGGFLSSFLLSTVSNITKRNGHGWILNNLNDSHLDYYYAFLALLSFVNLICFL 1134

BLAST of HG10012827.1 vs. ExPASy TrEMBL
Match: A0A151T5W9 (Peptide transporter PTR3-A OS=Cajanus cajan OX=3821 GN=KK1_016958 PE=3 SV=1)

HSP 1 Score: 1327.8 bits (3435), Expect = 0.0e+00
Identity = 649/1155 (56.19%), Postives = 865/1155 (74.89%), Query Frame = 0

Query: 13   DDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGT 72
            +DYT+DGTVD KG P+LRS TG WKACSFI+ YE+IERM + GIA+NL++YLT KL++GT
Sbjct: 6    EDYTQDGTVDLKGRPILRSNTGRWKACSFIVGYEMIERMAYYGIASNLVLYLTKKLHEGT 65

Query: 73   LTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPSLKP 132
            + +SN+VTNW GTVW+ P  GAY+ADAYLGRY TF I+S + L+ M LLTLAVS+P+L+P
Sbjct: 66   VKSSNHVTNWVGTVWMMPAAGAYIADAYLGRYWTFVIASAIYLLGMCLLTLAVSLPALRP 125

Query: 133  PPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQK 192
            PPC   I  ++C+ AS  Q+ +FF +LY++A  +GGTKPNISTMGADQFD+F+PKE+ QK
Sbjct: 126  PPCASGIADKDCQHASSFQVGIFFFALYIIAAGTGGTKPNISTMGADQFDEFEPKERTQK 185

Query: 193  LSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRL 252
            LSF+NWW+F++  G + A T+LVYIQD VG+ LGYGIPTIGL ++IL+F+ GTP YRHRL
Sbjct: 186  LSFYNWWVFNILIGTISAQTLLVYIQDRVGFGLGYGIPTIGLALSILVFLFGTPLYRHRL 245

Query: 253  PNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAA 312
            P+GSP  RM  V+VAA   W++ +P++ N+L EL ++                 FL+KAA
Sbjct: 246  PSGSPLTRMVQVLVAAMRKWKVKIPDNLNELNELSMED----------------FLDKAA 305

Query: 313  IRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLDRSIGS 372
            ++ G + PW LCTVT+VEETKQM++MIPI+I T IPST +AQ+ TLFI+QGTTLDRS+G 
Sbjct: 306  VKTGQTSPWMLCTVTQVEETKQMMKMIPILITTCIPSTTIAQTSTLFIRQGTTLDRSMGP 365

Query: 373  HFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICHILVMT 432
            HF++PPA L AFV I ML+S++IYDR+FV  +++ T NPRGI++LQR+GIG+  H+++M 
Sbjct: 366  HFEIPPACLIAFVNIFMLISVVIYDRLFVPTIRRYTNNPRGISLLQRLGIGLSLHVIIML 425

Query: 433  VASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEFFYDQA 492
             A  VE+ RL++A EN  +L  +   +PLTIFIL+PQF LTG+AD F+ +A  EFFYDQA
Sbjct: 426  TACLVERKRLSVAREN--NLLDQNDTIPLTIFILIPQFALTGIADTFVDVAKLEFFYDQA 485

Query: 493  PENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG-KGWILNNLNVSHLDYFYALLA 552
            PE MKSLG+SYF T+L IG+FLSTF+LS V+++T+R G KGWIL+NLNVS LDY+YA LA
Sbjct: 486  PEAMKSLGTSYFTTTLSIGSFLSTFLLSTVADLTRRHGHKGWILDNLNVSRLDYYYAFLA 545

Query: 553  VMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSKGLQQTDQESMVDDYTKDGTV 612
             +SA+NF  F++++                                              
Sbjct: 546  TLSAINFLCFVVVA---------------------------------------------- 605

Query: 613  DLKGKPLLRSKTGAWKACSFIIVYELMEKIMFHGIAANLIIYLTTKLHQGTVTASNNVTN 672
                KP+LRSKTG WKACSFI+ YE+ E++ F+GI +NL++YLT KLH+GTVTASNNV+N
Sbjct: 606  ----KPVLRSKTGKWKACSFIVGYEVFERMAFYGIQSNLVLYLTRKLHEGTVTASNNVSN 665

Query: 673  WSGTVWIMPILGAYIADAHLGRYRTFLISSFIWFTAMSLLTLAVSVPSLKPPPCLEAITK 732
            W G VW+MP+ GA+IADA+LGRY TF+ISS I+   M+LLTLAVS+ +L+PPPC++    
Sbjct: 666  WVGAVWMMPLAGAFIADAYLGRYWTFVISSGIYVLGMALLTLAVSLQALRPPPCVD---D 725

Query: 733  QNCKQASKLQLAVFFGSLYLLALASGGTKPNISTMGADQFDDFDPKEKAQKLSFFNWWLF 792
             NC  AS LQ  +FF  LY++A  +GGTKPNISTMGADQFDDF+PKE++ KLSFFNWW F
Sbjct: 726  HNCPHASSLQYGIFFLGLYIIAAGTGGTKPNISTMGADQFDDFEPKERSHKLSFFNWWFF 785

Query: 793  SVFSGFLFASTVLVYIQDNVGWSLGYGIPTIGIAIAILIFVVGTPFYRHRLPNGSPFTSM 852
            S+F G LFA+T L+YIQD VGW++GYG+PT+G+A ++L+F+VGTP+YRH+LP+GSP T M
Sbjct: 786  SIFFGTLFANTFLIYIQDYVGWTIGYGLPTLGLAFSVLVFLVGTPYYRHKLPSGSPITRM 845

Query: 853  ANVIVAAALNWRLPLPNHPNQLHELDLHHYSKPGTFKIDSTPSLRFLNKAAIRRDSSDPW 912
              V VAA   W+L + + P +LHEL +  Y+  G  +ID + S  FL+KAA++   + PW
Sbjct: 846  LQVFVAATRKWKLHVSDDPKELHELSIEEYASNGRNRIDKSSSFSFLDKAAVKTGQTSPW 905

Query: 913  RLCTVTEVEETKQMLRMIPILICTFIPNTIMAQTHTLFIKQGTTLNRSIGSHFKIPPASL 972
             LCTVT+VEETKQM ++IPI++ T +P+T++ QT TLF+KQG TL+RS+G HFKIPPA L
Sbjct: 906  MLCTVTQVEETKQMTKLIPIMLTTIVPSTLIVQTSTLFVKQGATLDRSMGPHFKIPPACL 965

Query: 973  NVFVTISMLLSILIYDRIFV-KMQRVTKNPRGITMLQRMGIGMICLVLVMTVASRVEKHR 1032
              FVT+SML++I++YDR+FV  ++R TKNPRGITMLQR+GIG++  V +M  A   E+ R
Sbjct: 966  TAFVTLSMLITIVMYDRLFVPAIRRYTKNPRGITMLQRLGIGLVLHVTIMVTACFAERKR 1025

Query: 1033 LKIVAATENGSSAQVLPLTIFILLPQFILTGFAEAFVQVAVMEFFYDQAPENMKSLGTSY 1092
            L +    +       +PLTIFILLPQ+ L G A+ FV+VA ME FYDQAPE MKSLGTSY
Sbjct: 1026 LSVAREKDLLEQKDAIPLTIFILLPQYALAGVADNFVEVAKMELFYDQAPEGMKSLGTSY 1085

Query: 1093 TMTSLGIGNFLSSLILSKVSEITKRQG-KGWILNNLNASHLDYFYALLAVMSAVNFFLFL 1152
              T+LGI +FLS+ +LS V++ITKR G KGW+L+NLN SHLDY+YA +A++S +NF  FL
Sbjct: 1086 FTTTLGIASFLSTFLLSTVADITKRNGHKGWVLDNLNVSHLDYYYAFMAILSFLNFLCFL 1089

Query: 1153 LISKLYVYKAEVSDS 1165
            + +K +VY  +V+ +
Sbjct: 1146 VAAKFFVYNVDVTQN 1089

BLAST of HG10012827.1 vs. TAIR 10
Match: AT5G46050.1 (peptide transporter 3 )

HSP 1 Score: 743.4 bits (1918), Expect = 2.8e-214
Identity = 363/580 (62.59%), Postives = 463/580 (79.83%), Query Frame = 0

Query: 8   QETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIK 67
           +E G DDYTKDGTVD +GNPV RS  G WKACSF++VYE+ ERM + GI++NL IY+T K
Sbjct: 4   EEVG-DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMTTK 63

Query: 68  LNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSV 127
           L+QGT+ +SNNVTNW GT W+TPILGAYV DA LGRY TF IS  +    M +LTL+V++
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSVTI 123

Query: 128 PSLKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPK 187
           P +KPP C    N ENC++AS LQLAVFFG+LY LAI +GGTKPNIST+GADQFD FDPK
Sbjct: 124 PGIKPPEC-STTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFDPK 183

Query: 188 EKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPF 247
           EK QKLSFFNWW+FS+F G LFA+T+LVY+QDNVGW+LGYG+PT+GL ++I IF++GTPF
Sbjct: 184 EKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGTPF 243

Query: 248 YRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRF 307
           YRH+LP GSPF +MA VIVA+      P+ +D    +EL    Y + G+F I  TPSLRF
Sbjct: 244 YRHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSLRF 303

Query: 308 LNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLD 367
           L++A+++ G++  W LCT TEVEETKQM+RM+P++  TF+PS M+AQ +TLF+KQGTTLD
Sbjct: 304 LDRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQGTTLD 363

Query: 368 RSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICH 427
           R +   F +PPASL  FVT+SML+SI++YDR+FVKI +K T NPRGIT+LQRMGIG+I H
Sbjct: 364 RKVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIGLIFH 423

Query: 428 ILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEF 487
           IL+M VAS  E++RL +AA++G  + Q    LPLTIF LLPQF+L G+AD+FL++A  EF
Sbjct: 424 ILIMIVASVTERYRLKVAADHG-LIHQTGVKLPLTIFALLPQFVLMGMADSFLEVAKLEF 483

Query: 488 FYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFY 547
           FYDQAPE+MKSLG+SY  TSL IGNF+S+F+LS VSEITK++G+GWILNNLN S LDY+Y
Sbjct: 484 FYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESRLDYYY 543

Query: 548 ALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKK 588
              AV++ VNF LFL++ K YVY+AEV+DS+ +   E+K+
Sbjct: 544 LFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of HG10012827.1 vs. TAIR 10
Match: AT5G46040.1 (Major facilitator superfamily protein )

HSP 1 Score: 718.4 bits (1853), Expect = 9.6e-207
Identity = 354/600 (59.00%), Postives = 466/600 (77.67%), Query Frame = 0

Query: 8   QETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIK 67
           +E G DDYTKDGTVD +GN V RS+TG WKACSF++VYE+ ERM + GI++NL+IY+T K
Sbjct: 4   EEVG-DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTTK 63

Query: 68  LNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSV 127
           L+QGT+ +SNNVTNW GT W+TPILGAYVADA+ GRY TF ISS + L+ M+LLTL+VS+
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVSL 123

Query: 128 PSLKPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPK 187
           P LKPP C  A N ENC++AS +QLAVFFG+LY LAI +GGTKPNIST+GADQFD+FDPK
Sbjct: 124 PGLKPPKCSTA-NVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPK 183

Query: 188 EKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPF 247
           +K  K SFFNWW+FS+F G  FA+T+LVY+QDNVGW++GYG+ T+GL  +I IF++GT  
Sbjct: 184 DKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRL 243

Query: 248 YRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRF 307
           YRH+LP GSPF +MA VIVA+    R P+ +D  + YEL    Y+   +F I ST SLRF
Sbjct: 244 YRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSLRF 303

Query: 308 LNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLD 367
           LN+A+++ GS+  WRLCT+TEVEETKQM++M+P++  TF+PS M+AQ  TLFIKQGTTLD
Sbjct: 304 LNRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQGTTLD 363

Query: 368 RSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICH 427
           R + ++F +PPASL  F T SML+SI+IYDR+FVK M+K+T NPRGIT+LQRMGIGMI H
Sbjct: 364 RRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIGMILH 423

Query: 428 ILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEF 487
           IL+M +AS  E++RL +AAE+G +  Q    +PL+IF LLPQ++L G+ADAF++IA  EF
Sbjct: 424 ILIMIIASITERYRLKVAAEHGLT-HQTAVPIPLSIFTLLPQYVLMGLADAFIEIAKLEF 483

Query: 488 FYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFY 547
           FYDQAPE+MKSLG+SY  TS+ +G F+S+ +LS VS+ITK+QG+GWI NNLN S LD +Y
Sbjct: 484 FYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESRLDNYY 543

Query: 548 ALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSKGLQQTDQESMVDDYTK 607
              AV++ +NF LFL++ + Y Y+A+V+ S  +              +  + +MVD+Y +
Sbjct: 544 MFFAVLNLLNFILFLVVIRFYEYRADVTQSANV--------------EQKEPNMVDNYNE 586

BLAST of HG10012827.1 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 568.2 bits (1463), Expect = 1.6e-161
Identity = 293/561 (52.23%), Postives = 404/561 (72.01%), Query Frame = 0

Query: 15  YTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGTLT 74
           YT+DGTVD +G PVL SKTG W+ACSF++ YE  ERM F GIA+NL+ YLT +L++ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 75  ASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPSLKPPP 134
           +  NV NW+G VWITPI GAY+AD+Y+GR+ TF  SSL+ ++ M LLT+AV+V SL+ P 
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLR-PT 126

Query: 135 CLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKLS 194
           C   +    C +AS LQ+  F+ SLY +AI +GGTKPNIST GADQFD +  +EK QK+S
Sbjct: 127 CENGV----CNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVS 186

Query: 195 FFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLPN 254
           FFNWW+FS F G LFA+  LVYIQ+N+GW LGYGIPT+GL V++++F +GTPFYRH++  
Sbjct: 187 FFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIK 246

Query: 255 GSPFIR-MANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAAI 314
                + +  V +AA  N +L  P+D  +LYELD  +Y  NG  ++  TP  RFL+KAAI
Sbjct: 247 TDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAI 306

Query: 315 RRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLDRSIGSH 374
           +  S  P   CTVT+VE  K+++ +I I + T IPST+ AQ +TLF+KQGTTLDR IGS+
Sbjct: 307 KTSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGSN 366

Query: 375 FKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICHILVMTV 434
           F++P ASL +FVT+SMLLS+ +YD+ FV  M+K T NPRGIT+LQR+G+G    I+ + +
Sbjct: 367 FQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIAI 426

Query: 435 ASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEFFYDQAP 494
           AS VE  R+ +  E    ++   +V+P++IF LLPQ+ L G+ D F  I   EFFYDQ+P
Sbjct: 427 ASAVEVKRMRVIKE--FHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQSP 486

Query: 495 ENMKSLGSSYFMTSLGIGNFLSTFILSKVSEIT-KRQGKGWILNNLNVSHLDYFYALLAV 554
           E M+SLG+++F + +G+GNFL++F+++ + +IT K  GK WI NNLN S LDY+Y  L V
Sbjct: 487 EEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFLVV 546

Query: 555 MSAVNFFLFLLISKLYVYKAE 574
           +S VN  LF+  +  YVYK++
Sbjct: 547 ISIVNMGLFVWAASKYVYKSD 557

BLAST of HG10012827.1 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 488.4 bits (1256), Expect = 1.6e-137
Identity = 258/570 (45.26%), Postives = 373/570 (65.44%), Query Frame = 0

Query: 13  DDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGT 72
           D YT+DGTVD   NP  + KTG+WKAC FI+  E  ER+ + G+  NL+ YL  +LNQG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 73  LTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPSLKP 132
            TA+NNVTNW+GT +ITP++GA++ADAYLGRY T      + +  M+LLTL+ SVP LKP
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 133 PPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQK 192
             C    N + C   S  Q AVFF +LY++A+ +GG KP +S+ GADQFD+ D  EK +K
Sbjct: 125 GNC----NADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKK 184

Query: 193 LSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRL 252
            SFFNW+ FS+  G L A+T+LV+IQ NVGW  G+G+PT+ + +A+  F  G+ FYR + 
Sbjct: 185 SSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQR 244

Query: 253 PNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAA 312
           P GSP  R+  VIVAA     + +P D + L+E      +  GS K+  T +L+F +KAA
Sbjct: 245 PGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAA 304

Query: 313 -------IRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTT 372
                  I+ G  +PWRLC+VT+VEE K ++ ++P+     + +T+ +Q  T+F+ QG T
Sbjct: 305 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 364

Query: 373 LDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMI 432
           +D+ +G +F++P ASL  F T+S+L    +YD+  + + +K T+N RG T LQRMGIG++
Sbjct: 365 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 424

Query: 433 CHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASN 492
             I  M  A  +E  RL+    +    + +QK + ++IF  +PQ++L G A+ F  I   
Sbjct: 425 VSIFAMITAGVLEVVRLDYVKTHN---AYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQL 484

Query: 493 EFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGK-GWILNNLNVSHLD 552
           EFFYDQAP+ M+SL S+  +T++ +GN+LST +++ V +ITK+ GK GWI +NLN  HLD
Sbjct: 485 EFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLD 544

Query: 553 YFYALLAVMSAVNFFLFLLISKLYVYKAEV 575
           YF+ LLA +S +NF ++L ISK Y YK  V
Sbjct: 545 YFFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of HG10012827.1 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 465.7 bits (1197), Expect = 1.1e-130
Identity = 253/565 (44.78%), Postives = 366/565 (64.78%), Query Frame = 0

Query: 15  YTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGTLT 74
           Y +DG+VD  GNP L+ KTG+WKAC FI+  E  ER+ + GIA NLI YLT KL+QG ++
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 75  ASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVAMSLLTLAVSVPSLKPPP 134
           A+ NVT W GT ++TP++GA +ADAY GRY T    S +  + MS LTL+ SVP+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 135 CLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKLS 194
           C+     + C  A+  Q A+FFG LYL+A+ +GG KP +S+ GADQFDD D +E+ +K S
Sbjct: 144 CI----GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKAS 203

Query: 195 FFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLPN 254
           FFNW+ FS+  G L +S++LV+IQ+N GW LG+GIPT+ +G+AI  F  GTP YR + P 
Sbjct: 204 FFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPG 263

Query: 255 GSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAAI- 314
           GSP  R++ V+VA+     + +P D   LYE   ++ +  GS KI+ T   ++L+KAA+ 
Sbjct: 264 GSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVI 323

Query: 315 -----RRGS-SDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLD 374
                + G  S+ WRLCTVT+VEE K ++RM PI     I S + AQ  T+F++QG  ++
Sbjct: 324 SEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMN 383

Query: 375 RSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICH 434
             IGS F++PPA+L  F T S+++ + +YDR  V + +K T   +G T +QRMGIG+   
Sbjct: 384 CKIGS-FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVS 443

Query: 435 ILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEF 494
           +L M  A+ VE  RL++A  N   L +    +P+++   +PQ+ + G A+ F  I   EF
Sbjct: 444 VLCMAAAAIVEIIRLHMA--NDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEF 503

Query: 495 FYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQG-KGWILNNLNVSHLDYF 554
           FYDQ+P+ M+SL S+  + +  +GN+LS+ IL+ V+  T R G +GWI +NLN  HLDYF
Sbjct: 504 FYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYF 563

Query: 555 YALLAVMSAVNFFLFLLISKLYVYK 572
           + LLA +S VN  ++   +  Y  K
Sbjct: 564 FWLLAGLSLVNMAVYFFSAARYKQK 581