HG10010233.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10010233.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Non-specific lipid-transfer protein-like protein
Location	Chr06: 20125918 .. 20127279 (-)
Sequence length	570
RNA-Seq Expression	HG10010233.1
Synteny	HG10010233.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATTCTTAGCCTCTTTTTATCACTCTTGGCCTTGGCTTTGGTGGCTATTCTTCCAGTGAATGGGCAGATGAATGTGCCATGTAGTGCCTCCATGCTCTCAAGCTTCAGCCCTTGCTTGAACTTTGTTACCAACAGCTCTGCCAATGGCACTTCCCCAACTGCCGATTGCTGTAACGCAATCCGGTCACTCGCAAGCGGCGGTCGAGACTGTCTCTGCCTCATCGTCACCGGACGAGTTCCCTTTCAAATACCTATCAATAGGACTCTTGCCATTTCACTTCCTCGCGCTTGTAACTTGCCTGGTGTCCCCCTCCAATGCAATGGTATATACTTTTCTAATTACTCATTAATCATCATAGTTATGGATTTGATCATGTCATTTGTTGATTTGAAACATAATCTTTAGATAATATCATTAGTGTTTTAATGATGTTAAATTTAGTGATTAGTGAATAACACTTGCCTATACTAGTAACTAGAAACTTCTTACTTATTGGCATACAAGCAAATAATGTTTAAGAGATTACTTATTAGAAATTTTCTTACTAATAAAATATATATATATATATATATATATATATATATTTTTAAAAAATAAGCAACAACATATCACTAATTTTATATAACATAAATAATAGACATTTTAATTTATTTAAGAATTGTAAAATGTTTATTTCCTATTTTTTTTTTATGAATTTTTTGCTTTTATAATTTTTCATGACATCAACATTTTTGATCTCAACATTTCAGTCCACATTGACATTTTAAACTTTGTTTTTACAAAAAGTGATACCATTAAAAAATAGAAATGGTATAAAAATAAACACAAAATTTAAAATTAAGAAACTAAAAAGTTTGATTGGATTTGTTATGAAATTACTAAACTGGGTACCCTTGTTTCAGCCGCTACTGCTCCTGTTCCCGCTCCAGGTACTATTTTCTAAACAAACTTCATCTTCTTCCTCAAGTTGATCTTATCATTGAATTTTCCATTTTTTTTCCCTGTTCATGCTCAATTAATAGTTAATGTTCTGAAATTGACAGGCCCAATTCCTTTTGGACCAAGCCTCTCTCCAGAATCTTCACCATCGGGCAGTCCTCAAGGTAATCAATAATCTCACTTCATTCAATCACTCTGCTTTCTGTTCTCCTCTCTAAAACTTGGCTTTTTGCAGCGCCCGAACCCACCACGCCGGCTCTTACTCCATCTTTTCCGGTGGTTCCTGAAGCTGGAGCTCCGTCGGAGACTGGCGGAAGTCGCCCAGTTCTCACTCCATCCTCCGCCGTCCCTTCAAACAGTTTTCCACATCCTTTTATGCTTCTGGGATTCGCATTTGTAATTTTGAAATTCAACTGA

mRNA sequence

ATGAAAATTCTTAGCCTCTTTTTATCACTCTTGGCCTTGGCTTTGGTGGCTATTCTTCCAGTGAATGGGCAGATGAATGTGCCATGTAGTGCCTCCATGCTCTCAAGCTTCAGCCCTTGCTTGAACTTTGTTACCAACAGCTCTGCCAATGGCACTTCCCCAACTGCCGATTGCTGTAACGCAATCCGGTCACTCGCAAGCGGCGGTCGAGACTGTCTCTGCCTCATCGTCACCGGACGAGTTCCCTTTCAAATACCTATCAATAGGACTCTTGCCATTTCACTTCCTCGCGCTTGTAACTTGCCTGGTGTCCCCCTCCAATGCAATGGCCCAATTCCTTTTGGACCAAGCCTCTCTCCAGAATCTTCACCATCGGGCAGTCCTCAAGCGCCCGAACCCACCACGCCGGCTCTTACTCCATCTTTTCCGGTGGTTCCTGAAGCTGGAGCTCCGTCGGAGACTGGCGGAAGTCGCCCAGTTCTCACTCCATCCTCCGCCGTCCCTTCAAACAGTTTTCCACATCCTTTTATGCTTCTGGGATTCGCATTTGTAATTTTGAAATTCAACTGA

Coding sequence (CDS)

ATGAAAATTCTTAGCCTCTTTTTATCACTCTTGGCCTTGGCTTTGGTGGCTATTCTTCCAGTGAATGGGCAGATGAATGTGCCATGTAGTGCCTCCATGCTCTCAAGCTTCAGCCCTTGCTTGAACTTTGTTACCAACAGCTCTGCCAATGGCACTTCCCCAACTGCCGATTGCTGTAACGCAATCCGGTCACTCGCAAGCGGCGGTCGAGACTGTCTCTGCCTCATCGTCACCGGACGAGTTCCCTTTCAAATACCTATCAATAGGACTCTTGCCATTTCACTTCCTCGCGCTTGTAACTTGCCTGGTGTCCCCCTCCAATGCAATGGCCCAATTCCTTTTGGACCAAGCCTCTCTCCAGAATCTTCACCATCGGGCAGTCCTCAAGCGCCCGAACCCACCACGCCGGCTCTTACTCCATCTTTTCCGGTGGTTCCTGAAGCTGGAGCTCCGTCGGAGACTGGCGGAAGTCGCCCAGTTCTCACTCCATCCTCCGCCGTCCCTTCAAACAGTTTTCCACATCCTTTTATGCTTCTGGGATTCGCATTTGTAATTTTGAAATTCAACTGA

Protein sequence

MKILSLFLSLLALALVAILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCNAIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCNGPIPFGPSLSPESSPSGSPQAPEPTTPALTPSFPVVPEAGAPSETGGSRPVLTPSSAVPSNSFPHPFMLLGFAFVILKFN

Homology

[Close]

BLAST of HG10010233.1 vs. NCBI nr
Match: XP_038874253.1 (non-specific lipid transfer protein GPI-anchored 19-like [Benincasa hispida])

HSP 1 Score: 325.5 bits (833), Expect = 3.1e-85
Identity = 174/197 (88.32%), Postives = 179/197 (90.86%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVAILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCN 60
           MK +S FL LLAL LVAILPV+GQMNVPCSASMLSSFSPCLNFVTNSSANGTSPT+DCCN
Sbjct: 1   MKFVSPFLLLLALPLVAILPVHGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTSDCCN 60

Query: 61  AIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN---------GP 120
           AIR LASGGRDCLC IVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN         GP
Sbjct: 61  AIRLLASGGRDCLCQIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCNASSAPVPAPGP 120

Query: 121 IPFGPSLSPESSPSGSPQAPEPTTPALTPSFPVVPEAGAPSETGGSRPVLTPSSAVPSNS 180
           IP GPSL PESSPSGSPQAPEPTTPALTPSFPV PEAGAPSETGGSRPVLTPSSAVPSNS
Sbjct: 121 IPLGPSLPPESSPSGSPQAPEPTTPALTPSFPVDPEAGAPSETGGSRPVLTPSSAVPSNS 180

Query: 181 FPHPFMLLGFAFVILKF 189
           FPHPF+LLGFAFVI+KF
Sbjct: 181 FPHPFLLLGFAFVIMKF 197

[Close]

BLAST of HG10010233.1 vs. NCBI nr
Match: XP_008447160.1 (PREDICTED: non-specific lipid-transfer protein-like protein At2g13820 [Cucumis melo] >KAA0040812.1 non-specific lipid-transfer protein-like protein [Cucumis melo var. makuwa] >TYK17789.1 non-specific lipid-transfer protein-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 295.4 bits (755), Expect = 3.4e-76
Identity = 163/197 (82.74%), Postives = 170/197 (86.29%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVA-ILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCC 60
           MK LS F  LLA ALVA ILPVNGQMN+ CSASM+SSFSPCLNFVTNSSANGTSPTADCC
Sbjct: 1   MKFLSPFFLLLAFALVAIILPVNGQMNMSCSASMVSSFSPCLNFVTNSSANGTSPTADCC 60

Query: 61  NAIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN---------G 120
           NAIRSLASGGRDCLCLIVTG VPFQIPINRTLAISLPRACNLPGVP+QCN          
Sbjct: 61  NAIRSLASGGRDCLCLIVTGGVPFQIPINRTLAISLPRACNLPGVPIQCNAATAPASAPA 120

Query: 121 PIPFGPSLSPESSPSGSPQAPEPTTPALTPSFPVVPEAGAPSETGGSRPVLTPSSAVPSN 180
            IP GPSLSP+ SP+ SPQAPEPTTPALTPS PVVPEA APSETGGSRPVLTPSSAVPSN
Sbjct: 121 SIPLGPSLSPQPSPTASPQAPEPTTPALTPSSPVVPEAEAPSETGGSRPVLTPSSAVPSN 180

Query: 181 SFPHPFMLLGFAFVILK 188
           SFP  F+L+G AFVILK
Sbjct: 181 SFPDSFLLMGLAFVILK 197

[Close]

BLAST of HG10010233.1 vs. NCBI nr
Match: XP_011659016.1 (non-specific lipid-transfer protein-like protein At2g13820 [Cucumis sativus] >KGN44233.1 hypothetical protein Csa_015516 [Cucumis sativus])

HSP 1 Score: 283.1 bits (723), Expect = 1.8e-72
Identity = 156/199 (78.39%), Postives = 167/199 (83.92%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVAIL---PVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTAD 60
           MK +S F  LLA  LVAI+    VNGQMN+ CSASM+S+FSPCLNFVTNSSANGTSPT+D
Sbjct: 1   MKFVSPFFLLLAFTLVAIILPHSVNGQMNMSCSASMVSTFSPCLNFVTNSSANGTSPTSD 60

Query: 61  CCNAIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN-------- 120
           CCNAIRSLASGGRDCLCLIVTG VPFQIPINRTLAISLPRACNLPGVPLQCN        
Sbjct: 61  CCNAIRSLASGGRDCLCLIVTGGVPFQIPINRTLAISLPRACNLPGVPLQCNAATAPASA 120

Query: 121 -GPIPFGPSLSPESSPSGSPQAPEPTTPALTPSFPVVPEAGAPSETGGSRPVLTPSSAVP 180
              IP GP+L P+ SPS SPQAPEPTTPALTPS PVVPEA APSETGGSRPVLTPSS VP
Sbjct: 121 PASIPLGPTLPPQPSPSASPQAPEPTTPALTPSSPVVPEAEAPSETGGSRPVLTPSSDVP 180

Query: 181 SNSFPHPFMLLGFAFVILK 188
           SNSFP+ F+L+G AFVILK
Sbjct: 181 SNSFPNTFLLMGLAFVILK 199

[Close]

BLAST of HG10010233.1 vs. NCBI nr
Match: KAG6574973.1 (hypothetical protein SDJN03_25612, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 278.9 bits (712), Expect = 3.3e-71
Identity = 158/204 (77.45%), Postives = 168/204 (82.35%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVAILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCN 60
           MK+L  FL LLALALVA+LP NGQ N PC+ASMLSSF+PC+NF++NSSANGTSPTADCCN
Sbjct: 1   MKLLGPFLPLLALALVAVLPANGQTNTPCTASMLSSFTPCMNFLSNSSANGTSPTADCCN 60

Query: 61  AIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN---------GP 120
           AIRSLASGGRDCLCLIVTG VPFQIPINRTLAISLPRACNLPGVPLQCN         GP
Sbjct: 61  AIRSLASGGRDCLCLIVTGGVPFQIPINRTLAISLPRACNLPGVPLQCNAAAAPVPAPGP 120

Query: 121 IPFGPSLSPESSPSGSPQAPEPTTPAL-----TPSFPVVPEAGAPSETGGSRPVLTP-SS 180
           I  GPS  PESSPS SPQAPEPTTPAL     TPS P+VP+A AP+ T GSRPVLTP SS
Sbjct: 121 IALGPSFPPESSPSASPQAPEPTTPALTPVAETPSSPLVPDAEAPTGTAGSRPVLTPSSS 180

Query: 181 AVPSNSFPHPFMLLGFAFVILKFN 190
           AV SNSF  PFM LGF FVILKFN
Sbjct: 181 AVRSNSF--PFMFLGFVFVILKFN 202

[Close]

BLAST of HG10010233.1 vs. NCBI nr
Match: XP_023549160.1 (non-specific lipid-transfer protein-like protein At2g13820 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 278.5 bits (711), Expect = 4.3e-71
Identity = 158/204 (77.45%), Postives = 167/204 (81.86%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVAILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCN 60
           MK+L  FL LLALALVA+LP NGQ N PC+ASMLSSF+PC+NF+ NSSANGTSPTADCCN
Sbjct: 1   MKLLGPFLPLLALALVAVLPANGQTNTPCTASMLSSFTPCMNFLANSSANGTSPTADCCN 60

Query: 61  AIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN---------GP 120
           AIRSLASGGRDCLCLIVTG VPFQIPINRTLAISLPRACNLPGVPLQCN         GP
Sbjct: 61  AIRSLASGGRDCLCLIVTGGVPFQIPINRTLAISLPRACNLPGVPLQCNAAAAPVPAPGP 120

Query: 121 IPFGPSLSPESSPSGSPQAPEPTTPAL-----TPSFPVVPEAGAPSETGGSRPVLTP-SS 180
           I  GPS  PESSPS SPQAPEPTTPAL     TPS P+VPEA AP+ T GSRPVLTP SS
Sbjct: 121 IALGPSFPPESSPSASPQAPEPTTPALTPVAETPSSPLVPEAEAPTGTAGSRPVLTPSSS 180

Query: 181 AVPSNSFPHPFMLLGFAFVILKFN 190
           AV SNSF  PF+ LGF FVILKFN
Sbjct: 181 AVRSNSF--PFLFLGFVFVILKFN 202

[Close]

BLAST of HG10010233.1 vs. ExPASy Swiss-Prot
Match: Q9LJ85 (Non-specific lipid transfer protein GPI-anchored 20 OS=Arabidopsis thaliana OX=3702 GN=LTPG20 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.7e-36
Identity = 103/208 (49.52%), Postives = 129/208 (62.02%), Query Frame = 0


Query: 2   KILSLFLSLLALALVAILPVNGQMN--VPCSASMLSSFSPCLNFVTNSSANGTSPTADCC 61
           KI+SL ++++A   V  LP+ GQ      C+ SM+++ SPC+ F+TNSS+NGTSP++DCC
Sbjct: 3   KIISLVVAMIA---VLALPIRGQQQPLSQCTPSMMTTVSPCMGFITNSSSNGTSPSSDCC 62

Query: 62  NAIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCNGPI------- 121
           N++RSL +GG  CLCLIVTG VPF IPINRT A+SLPRACN+P VPLQC   I       
Sbjct: 63  NSLRSLTTGGMGCLCLIVTGTVPFNIPINRTTAVSLPRACNMPRVPLQCQANIAPAAAPG 122

Query: 122 ---PFGPSLSPESSPSGSPQAPEPTTPALTP----SFPVVP--EAGAP-SETGG--SRPV 181
               FGPS+SP   P   P  PEPT  A TP    + P  P  + GAP S+ GG  SRP 
Sbjct: 123 PAATFGPSMSP--GPETDPIVPEPTPAAQTPQSDTTRPFTPSVDGGAPTSDDGGSTSRPS 182

Query: 182 LTPSSAVPSNSFPHPFMLLGFAFVILKF 189
            TPSSA    +     +    A V LKF
Sbjct: 183 ETPSSAY---ALSPSLLFFSIALVALKF 202

[Close]

BLAST of HG10010233.1 vs. ExPASy Swiss-Prot
Match: Q1G2Y5 (Non-specific lipid transfer protein GPI-anchored 21 OS=Arabidopsis thaliana OX=3702 GN=LTPG21 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.6e-30
Identity = 84/180 (46.67%), Postives = 105/180 (58.33%), Query Frame = 0


Query: 24  QMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCNAIRSLASGGRDCLCLIVTGRVPF 83
           Q+N PCS SMLSS + C +F+T     G+ PT+DCC A++SL   G DCLCLIVT  VP 
Sbjct: 28  QINTPCSPSMLSSVTGCTSFLT---GGGSFPTSDCCGALKSLTGTGMDCLCLIVTAGVPI 87

Query: 84  QIPINRTLAISLPRACNLPGVPLQCN---------GPIPFGPSLSP----ESSPSGSPQA 143
            IPINRTLAISLPRAC +PGVP+QC          GP  FGP+ SP     S P GS   
Sbjct: 88  SIPINRTLAISLPRACGIPGVPVQCKASAAPLPTPGPASFGPTTSPTDSQTSDPEGSASF 147

Query: 144 PEPTTP--ALTPSFPVVPEAGAPSETGGSRPVLTPSSAVPSNSFPHPFMLLGFAFVILKF 189
             PT+P  + TP+   +  +G   +  G  P    SS   S+S    ++L  FAF I+KF
Sbjct: 148 RPPTSPTTSQTPNDKDLSGSGNGGDPMGFAPPPPSSSPSSSHSLKLSYLLFAFAFTIIKF 204

[Close]

BLAST of HG10010233.1 vs. ExPASy Swiss-Prot
Match: Q94AX3 (Non-specific lipid transfer protein GPI-anchored 16 OS=Arabidopsis thaliana OX=3702 GN=LTPG16 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.5e-21
Identity = 71/177 (40.11%), Postives = 98/177 (55.37%), Query Frame = 0


Query: 24  QMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCNAIRSLASGGRDCLCLIVTGRVPF 83
           Q++ PC++SM+S+F+PCLNF+T SS    +PTA CC+++++L + G  C CLI+T  VP 
Sbjct: 22  QISTPCTSSMISTFTPCLNFITGSSGGSVTPTAGCCDSLKTLTNTGMGCACLILTANVPL 81

Query: 84  QIP-INRTLAISLPRACNLPGVPLQCN---------GPIPFGPSLSPE---SSPSGSPQA 143
               INRTLA++LPRAC + GVP+QC          G +PF  +  P+    SP  S  A
Sbjct: 82  PTGFINRTLALALPRACKMGGVPIQCQAAGTPLPAPGQVPFLIAPPPQVSAFSPGASKAA 141

Query: 144 PEPTTPALTPSFPVVPEAGAPSETGGSRPVLTPSSAVP-SNSFPHPFMLLGFAFVIL 187
               T A  P  P     G P+   G RPV  P      + S   PF+ L F  +IL
Sbjct: 142 GTTPTQAPAPDTPADGPTG-PTTKSGIRPVDQPMQPTGLAQSSTSPFLPLLFISLIL 197

[Close]

BLAST of HG10010233.1 vs. ExPASy Swiss-Prot
Match: Q7EB72 (Non-specific lipid transfer protein GPI-anchored 15 OS=Arabidopsis thaliana OX=3702 GN=LTPG15 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.2e-09
Identity = 62/183 (33.88%), Postives = 88/183 (48.09%), Query Frame = 0


Query: 13  LALVAIL--PVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCNAIRSLASGGR 72
           +ALVA L      Q +  C  S L++ SPCL+++T    N T+P+  CC+ + S+     
Sbjct: 12  VALVAALWSVTKAQPSSSC-VSTLTTLSPCLSYIT---GNSTTPSQPCCSRLDSVIKSSP 71

Query: 73  DCLCLIVTGRVP-FQIPINRTLAISLPRACNLPGVPL-QCNGPIPFGPSLSPESSPSGSP 132
            C+C  V   +P   + INRT A+ LP ACN+   PL QCN            + P+  P
Sbjct: 72  QCICSAVNSPIPNIGLNINRTQALQLPNACNIQTPPLTQCNA----------ATGPTAQP 131

Query: 133 QAPEPTTPALTPSFPVVPEA--GAPSETGGSRPVLTPSSAVPSNS---FPHPFMLLGFAF 187
            AP PT    TP   + P +  GA S  GG    + PS    S+S    P P   L FA 
Sbjct: 132 PAPSPTEK--TPDVTLTPTSLPGARSGVGGGSKTV-PSVGTGSSSRNVDPLPLHFLMFAV 177

[Close]

BLAST of HG10010233.1 vs. ExPASy Swiss-Prot
Match: Q9LJ86 (Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=3702 GN=LTPG5 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.6e-07
Identity = 55/159 (34.59%), Postives = 85/159 (53.46%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVAILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCN 60
           M++  +FL++  +A+++   V+ Q +  C+ +++ S SPCLN++T    N TSP   CCN
Sbjct: 3   MEMGLVFLTVF-MAVMSSTMVSAQSS--CTNALI-SMSPCLNYIT---GNSTSPNQQCCN 62

Query: 61  AIRSLASGGRDCLCLIVT-GRVPFQIPINRTLAISLPRACNLPGVPL-QCNGPIPFGPSL 120
            +  +     DCLC ++  G     I +N+T A+ LPRACN+   P+ +CN     G S 
Sbjct: 63  QLSRVVQSSPDCLCQVLNGGGSQLGINVNQTQALGLPRACNVQTPPVSRCNTGGGGGGST 122

Query: 121 SPESSPSGSPQAPEPTTPALTPSFPVVPEAGAPSETGGS 158
           S   SP+ SP +  P   + T   PV    G PS  G S
Sbjct: 123 S--DSPAESPNSSGPGNGSKT--VPVGEGDGPPSSDGSS 150

[Close]

BLAST of HG10010233.1 vs. ExPASy TrEMBL
Match: A0A5D3D3P8 (Non-specific lipid-transfer protein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G00330 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.7e-76
Identity = 163/197 (82.74%), Postives = 170/197 (86.29%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVA-ILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCC 60
           MK LS F  LLA ALVA ILPVNGQMN+ CSASM+SSFSPCLNFVTNSSANGTSPTADCC
Sbjct: 1   MKFLSPFFLLLAFALVAIILPVNGQMNMSCSASMVSSFSPCLNFVTNSSANGTSPTADCC 60

Query: 61  NAIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN---------G 120
           NAIRSLASGGRDCLCLIVTG VPFQIPINRTLAISLPRACNLPGVP+QCN          
Sbjct: 61  NAIRSLASGGRDCLCLIVTGGVPFQIPINRTLAISLPRACNLPGVPIQCNAATAPASAPA 120

Query: 121 PIPFGPSLSPESSPSGSPQAPEPTTPALTPSFPVVPEAGAPSETGGSRPVLTPSSAVPSN 180
            IP GPSLSP+ SP+ SPQAPEPTTPALTPS PVVPEA APSETGGSRPVLTPSSAVPSN
Sbjct: 121 SIPLGPSLSPQPSPTASPQAPEPTTPALTPSSPVVPEAEAPSETGGSRPVLTPSSAVPSN 180

Query: 181 SFPHPFMLLGFAFVILK 188
           SFP  F+L+G AFVILK
Sbjct: 181 SFPDSFLLMGLAFVILK 197

[Close]

BLAST of HG10010233.1 vs. ExPASy TrEMBL
Match: A0A1S3BGS3 (non-specific lipid-transfer protein-like protein At2g13820 OS=Cucumis melo OX=3656 GN=LOC103489676 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.7e-76
Identity = 163/197 (82.74%), Postives = 170/197 (86.29%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVA-ILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCC 60
           MK LS F  LLA ALVA ILPVNGQMN+ CSASM+SSFSPCLNFVTNSSANGTSPTADCC
Sbjct: 1   MKFLSPFFLLLAFALVAIILPVNGQMNMSCSASMVSSFSPCLNFVTNSSANGTSPTADCC 60

Query: 61  NAIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN---------G 120
           NAIRSLASGGRDCLCLIVTG VPFQIPINRTLAISLPRACNLPGVP+QCN          
Sbjct: 61  NAIRSLASGGRDCLCLIVTGGVPFQIPINRTLAISLPRACNLPGVPIQCNAATAPASAPA 120

Query: 121 PIPFGPSLSPESSPSGSPQAPEPTTPALTPSFPVVPEAGAPSETGGSRPVLTPSSAVPSN 180
            IP GPSLSP+ SP+ SPQAPEPTTPALTPS PVVPEA APSETGGSRPVLTPSSAVPSN
Sbjct: 121 SIPLGPSLSPQPSPTASPQAPEPTTPALTPSSPVVPEAEAPSETGGSRPVLTPSSAVPSN 180

Query: 181 SFPHPFMLLGFAFVILK 188
           SFP  F+L+G AFVILK
Sbjct: 181 SFPDSFLLMGLAFVILK 197

[Close]

BLAST of HG10010233.1 vs. ExPASy TrEMBL
Match: A0A0A0K3Y9 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G232570 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 8.5e-73
Identity = 156/199 (78.39%), Postives = 167/199 (83.92%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVAIL---PVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTAD 60
           MK +S F  LLA  LVAI+    VNGQMN+ CSASM+S+FSPCLNFVTNSSANGTSPT+D
Sbjct: 1   MKFVSPFFLLLAFTLVAIILPHSVNGQMNMSCSASMVSTFSPCLNFVTNSSANGTSPTSD 60

Query: 61  CCNAIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN-------- 120
           CCNAIRSLASGGRDCLCLIVTG VPFQIPINRTLAISLPRACNLPGVPLQCN        
Sbjct: 61  CCNAIRSLASGGRDCLCLIVTGGVPFQIPINRTLAISLPRACNLPGVPLQCNAATAPASA 120

Query: 121 -GPIPFGPSLSPESSPSGSPQAPEPTTPALTPSFPVVPEAGAPSETGGSRPVLTPSSAVP 180
              IP GP+L P+ SPS SPQAPEPTTPALTPS PVVPEA APSETGGSRPVLTPSS VP
Sbjct: 121 PASIPLGPTLPPQPSPSASPQAPEPTTPALTPSSPVVPEAEAPSETGGSRPVLTPSSDVP 180

Query: 181 SNSFPHPFMLLGFAFVILK 188
           SNSFP+ F+L+G AFVILK
Sbjct: 181 SNSFPNTFLLMGLAFVILK 199

[Close]

BLAST of HG10010233.1 vs. ExPASy TrEMBL
Match: A0A6J1H4D0 (non-specific lipid-transfer protein-like protein At2g13820 OS=Cucurbita moschata OX=3662 GN=LOC111460366 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 2.1e-71
Identity = 158/204 (77.45%), Postives = 167/204 (81.86%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVAILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCN 60
           MK+L  FL LLALALVA+LP NGQ N PC+ASMLSSF+PC+NF+ NSSANGTSPTADCCN
Sbjct: 1   MKLLGPFLPLLALALVAVLPANGQTNTPCTASMLSSFTPCMNFLANSSANGTSPTADCCN 60

Query: 61  AIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN---------GP 120
           AIRSLASGGRDCLCLIVTG VPFQIPINRTLAISLPRACNLPGVPLQCN         GP
Sbjct: 61  AIRSLASGGRDCLCLIVTGGVPFQIPINRTLAISLPRACNLPGVPLQCNAAAAPVPAPGP 120

Query: 121 IPFGPSLSPESSPSGSPQAPEPTTPAL-----TPSFPVVPEAGAPSETGGSRPVLTP-SS 180
           I  GPS  PESSPS SPQAPEPTTPAL     TPS P+VP+A AP+ T GSRPVLTP SS
Sbjct: 121 IALGPSFPPESSPSASPQAPEPTTPALTPVAETPSSPLVPDAEAPTGTAGSRPVLTPSSS 180

Query: 181 AVPSNSFPHPFMLLGFAFVILKFN 190
           AV SNSF  PFM LGF FVILKFN
Sbjct: 181 AVRSNSF--PFMFLGFVFVILKFN 202

[Close]

BLAST of HG10010233.1 vs. ExPASy TrEMBL
Match: A0A6J1KWN9 (non-specific lipid-transfer protein-like protein At2g13820 OS=Cucurbita maxima OX=3661 GN=LOC111499392 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 4.7e-71
Identity = 157/204 (76.96%), Postives = 167/204 (81.86%), Query Frame = 0


Query: 1   MKILSLFLSLLALALVAILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCN 60
           MK+L  FL LLALALVA+LP NGQ N PC+ASMLSSF+PC+NF+ NSSANGTSPTADCCN
Sbjct: 1   MKLLGPFLPLLALALVAVLPANGQTNAPCTASMLSSFTPCMNFLANSSANGTSPTADCCN 60

Query: 61  AIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCN---------GP 120
           AIRSLASGGRDCLCLIVTG VPFQIPINRTLAISLPRACNLPGVPLQCN         GP
Sbjct: 61  AIRSLASGGRDCLCLIVTGGVPFQIPINRTLAISLPRACNLPGVPLQCNAAAAPVPAPGP 120

Query: 121 IPFGPSLSPESSPSGSPQAPEPTTPAL-----TPSFPVVPEAGAPSETGGSRPVLTP-SS 180
           I  GPS  PESSPS SPQAPEPTTPAL     TPS P+VPEA +P+ T GSRPVLTP SS
Sbjct: 121 IALGPSFPPESSPSASPQAPEPTTPALTPVAETPSSPLVPEAESPTGTAGSRPVLTPSSS 180

Query: 181 AVPSNSFPHPFMLLGFAFVILKFN 190
           AV SNSF  PF+ LGF FVILKFN
Sbjct: 181 AVRSNSF--PFLFLGFVFVILKFN 202

[Close]

BLAST of HG10010233.1 vs. TAIR 10
Match: AT3G22620.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.0e-37
Identity = 103/208 (49.52%), Postives = 129/208 (62.02%), Query Frame = 0


Query: 2   KILSLFLSLLALALVAILPVNGQMN--VPCSASMLSSFSPCLNFVTNSSANGTSPTADCC 61
           KI+SL ++++A   V  LP+ GQ      C+ SM+++ SPC+ F+TNSS+NGTSP++DCC
Sbjct: 3   KIISLVVAMIA---VLALPIRGQQQPLSQCTPSMMTTVSPCMGFITNSSSNGTSPSSDCC 62

Query: 62  NAIRSLASGGRDCLCLIVTGRVPFQIPINRTLAISLPRACNLPGVPLQCNGPI------- 121
           N++RSL +GG  CLCLIVTG VPF IPINRT A+SLPRACN+P VPLQC   I       
Sbjct: 63  NSLRSLTTGGMGCLCLIVTGTVPFNIPINRTTAVSLPRACNMPRVPLQCQANIAPAAAPG 122

Query: 122 ---PFGPSLSPESSPSGSPQAPEPTTPALTP----SFPVVP--EAGAP-SETGG--SRPV 181
               FGPS+SP   P   P  PEPT  A TP    + P  P  + GAP S+ GG  SRP 
Sbjct: 123 PAATFGPSMSP--GPETDPIVPEPTPAAQTPQSDTTRPFTPSVDGGAPTSDDGGSTSRPS 182

Query: 182 LTPSSAVPSNSFPHPFMLLGFAFVILKF 189
            TPSSA    +     +    A V LKF
Sbjct: 183 ETPSSAY---ALSPSLLFFSIALVALKF 202

[Close]

BLAST of HG10010233.1 vs. TAIR 10
Match: AT1G05450.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 4.7e-31
Identity = 84/180 (46.67%), Postives = 105/180 (58.33%), Query Frame = 0


Query: 24  QMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCNAIRSLASGGRDCLCLIVTGRVPF 83
           Q+N PCS SMLSS + C +F+T     G+ PT+DCC A++SL   G DCLCLIVT  VP 
Sbjct: 28  QINTPCSPSMLSSVTGCTSFLT---GGGSFPTSDCCGALKSLTGTGMDCLCLIVTAGVPI 87

Query: 84  QIPINRTLAISLPRACNLPGVPLQCN---------GPIPFGPSLSP----ESSPSGSPQA 143
            IPINRTLAISLPRAC +PGVP+QC          GP  FGP+ SP     S P GS   
Sbjct: 88  SIPINRTLAISLPRACGIPGVPVQCKASAAPLPTPGPASFGPTTSPTDSQTSDPEGSASF 147

Query: 144 PEPTTP--ALTPSFPVVPEAGAPSETGGSRPVLTPSSAVPSNSFPHPFMLLGFAFVILKF 189
             PT+P  + TP+   +  +G   +  G  P    SS   S+S    ++L  FAF I+KF
Sbjct: 148 RPPTSPTTSQTPNDKDLSGSGNGGDPMGFAPPPPSSSPSSSHSLKLSYLLFAFAFTIIKF 204

[Close]

BLAST of HG10010233.1 vs. TAIR 10
Match: AT2G48140.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 103.6 bits (257), Expect = 1.8e-22
Identity = 71/177 (40.11%), Postives = 98/177 (55.37%), Query Frame = 0


Query: 24  QMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCNAIRSLASGGRDCLCLIVTGRVPF 83
           Q++ PC++SM+S+F+PCLNF+T SS    +PTA CC+++++L + G  C CLI+T  VP 
Sbjct: 22  QISTPCTSSMISTFTPCLNFITGSSGGSVTPTAGCCDSLKTLTNTGMGCACLILTANVPL 81

Query: 84  QIP-INRTLAISLPRACNLPGVPLQCN---------GPIPFGPSLSPE---SSPSGSPQA 143
               INRTLA++LPRAC + GVP+QC          G +PF  +  P+    SP  S  A
Sbjct: 82  PTGFINRTLALALPRACKMGGVPIQCQAAGTPLPAPGQVPFLIAPPPQVSAFSPGASKAA 141

Query: 144 PEPTTPALTPSFPVVPEAGAPSETGGSRPVLTPSSAVP-SNSFPHPFMLLGFAFVIL 187
               T A  P  P     G P+   G RPV  P      + S   PF+ L F  +IL
Sbjct: 142 GTTPTQAPAPDTPADGPTG-PTTKSGIRPVDQPMQPTGLAQSSTSPFLPLLFISLIL 197

[Close]

BLAST of HG10010233.1 vs. TAIR 10
Match: AT2G48140.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 6.7e-22
Identity = 51/107 (47.66%), Postives = 74/107 (69.16%), Query Frame = 0


Query: 24  QMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCNAIRSLASGGRDCLCLIVTGRVPF 83
           Q++ PC++SM+S+F+PCLNF+T SS    +PTA CC+++++L + G  C CLI+T  VP 
Sbjct: 22  QISTPCTSSMISTFTPCLNFITGSSGGSVTPTAGCCDSLKTLTNTGMGCACLILTANVPL 81

Query: 84  QIP-INRTLAISLPRACNLPGVPLQCNG---PIPFGPSLSPESSPSG 127
               INRTLA++LPRAC + GVP+QC     P+P   S+   S+ SG
Sbjct: 82  PTGFINRTLALALPRACKMGGVPIQCQAAGTPLPAPGSIFDCSTTSG 128

[Close]

BLAST of HG10010233.1 vs. TAIR 10
Match: AT1G73890.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 42.0 bits (97), Expect = 6.3e-04
Identity = 51/162 (31.48%), Postives = 74/162 (45.68%), Query Frame = 0


Query: 3   ILSLFLSLLALALVAILPVNGQMNVPCSASMLSSFSPCLNFVTNSSANGTSPTADCCNAI 62
           +++L +SL A  L  +L    Q+   C AS L S +PC  FV   +     P   CC+++
Sbjct: 7   LITLLISLSAFFLRMVL---AQVPATC-ASRLLSLAPCGPFVQGFA---QLPAQPCCDSL 66

Query: 63  RSLASGGRDCLCLIV--TGRVPFQIPINRTLAISLPRACNLPGVPLQCNGPIP-FGPSLS 122
             + S    CLCL +  T  +    PIN+TLA+ LP  CN+P     C+   P   PS S
Sbjct: 67  NQIYSQEATCLCLFLNNTSTLSPAFPINQTLALQLPPLCNIPANSSTCSSSFPGEAPSDS 126

Query: 123 -----PESSPSGSPQAPEPTTPALTPSFPVVPEAGAPSETGG 157
                P SS +GS  +      +   + PV   A  P+   G
Sbjct: 127 SSVAPPPSSSTGSQISQGAKNNSRVAATPVAQMAPRPTSFMG 161

The following BLAST results are available for this feature:

BLAST of HG10010233.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038874253.1	3.1e-85	88.32	non-specific lipid transfer protein GPI-anchored 19-like [Benincasa hispida]	[more]
XP_008447160.1	3.4e-76	82.74	PREDICTED: non-specific lipid-transfer protein-like protein At2g13820 [Cucumis m...	[more]
XP_011659016.1	1.8e-72	78.39	non-specific lipid-transfer protein-like protein At2g13820 [Cucumis sativus] >KG...	[more]
KAG6574973.1	3.3e-71	77.45	hypothetical protein SDJN03_25612, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023549160.1	4.3e-71	77.45	non-specific lipid-transfer protein-like protein At2g13820 [Cucurbita pepo subsp...	[more]

BLAST of HG10010233.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LJ85	2.7e-36	49.52	Non-specific lipid transfer protein GPI-anchored 20 OS=Arabidopsis thaliana OX=3...	[more]
Q1G2Y5	6.6e-30	46.67	Non-specific lipid transfer protein GPI-anchored 21 OS=Arabidopsis thaliana OX=3...	[more]
Q94AX3	2.5e-21	40.11	Non-specific lipid transfer protein GPI-anchored 16 OS=Arabidopsis thaliana OX=3...	[more]
Q7EB72	2.2e-09	33.88	Non-specific lipid transfer protein GPI-anchored 15 OS=Arabidopsis thaliana OX=3...	[more]
Q9LJ86	1.6e-07	34.59	Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of HG10010233.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D3P8	1.7e-76	82.74	Non-specific lipid-transfer protein-like protein OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BGS3	1.7e-76	82.74	non-specific lipid-transfer protein-like protein At2g13820 OS=Cucumis melo OX=36...	[more]
A0A0A0K3Y9	8.5e-73	78.39	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G232570 PE=4 SV...	[more]
A0A6J1H4D0	2.1e-71	77.45	non-specific lipid-transfer protein-like protein At2g13820 OS=Cucurbita moschata...	[more]
A0A6J1KWN9	4.7e-71	76.96	non-specific lipid-transfer protein-like protein At2g13820 OS=Cucurbita maxima O...	[more]

BLAST of HG10010233.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22620.1	2.0e-37	49.52	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G05450.2	4.7e-31	46.67	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G48140.1	1.8e-22	40.11	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G48140.2	6.7e-22	47.66	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G73890.1	6.3e-04	31.48	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 29..108 e-value: 1.5E-6 score: 37.8
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 13..108 e-value: 3.2E-17 score: 62.5
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 27..108 e-value: 1.9E-14 score: 55.3
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 33..103
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 126..140
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 117..162
None	No IPR available	PANTHER	PTHR33044:SF136	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 12..188
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 37..101 e-value: 6.54184E-14 score: 61.6739
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 12..188

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10010233	HG10010233	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10010233.1-cds	HG10010233.1-cds-Chr06:20125918..20126099	CDS
HG10010233.1-cds	HG10010233.1-cds-Chr06:20126171..20126230	CDS
HG10010233.1-cds	HG10010233.1-cds-Chr06:20126952..20127279	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10010233.1	HG10010233.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0005886	plasma membrane