HG10008044.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10008044.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Pyruvate kinase
Location	Chr10: 19044856 .. 19046108 (+)
Sequence length	441
RNA-Seq Expression	HG10008044.1
Synteny	HG10008044.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAATATTGATATTGAGGGAATTCTTACCCCAAAACAAAAATTTGTACCTGCTTCTCGATCGTACATGAGTATCATCGAGAGACTCAAAATTTGTACGTACATGAGTATCATCAAGAGACTTGAGATAACAATCTCAGGACCGCCGTGCAGAATACTCAAATCATGCTTGATACTAAGGTCTGTTACTTTCTCCTGCTACGATCTTTGCTTGAAACAGATGAGATATGCGATGATGTGAATCATGTGACCGTATTTTATTAGATTCCGTAAATGTTTATTATTGGTTCTGGCTTGGGTGCTCTGGAAATGGATGGAAGAAGGATCTGGTTAATGAACTTACTTTCACCCATTTGTTGTGGATGAATGATTTTGGGCAATGAAAAAATTAACTGCCTTCTCAATTCACTTGCTAGTGCAACAATGACACATTTGTAGGAGAACCACCACATTTATACCATGCTGAAAGTATACGATTACCCTGTTGCCTCTCTTGCAAAGAAGTAAAATGTGGGCTCTATTTAAAATTTAGAAAACCTACTGAGGATCTAGAACTGAGGATAGTTAGTTCGCCACTATATTTCGATTTATTATTATGGACCTAAATAAGGTGTTGCGATCTAGTATCTACAATCTTTTTGCAACTTTTCATTTACAATTCTAAGTTCTAATTATTATAGGGCTTTATAAATCTCTAAGCTAGTTCAGGGATATTTTTTTTTCTAGGTAAAGATTGAACTTGGGATTGAATAATACACTTCTAGTTCTTTTGCTTATGAAAGAAAGTATGTACTTACTAAAAAAAGAATCGTATTAACACTTATTATGAGAAAGAGGATTGAATATGCAATTCCTATTAACACTTATTATGAGAAAGAGGATTGAATATGCAATTCCTATTAAAAAAACACTGCGTACACCAACTCATGCGCTTTTGAAGAGTTCAACTCATCCTGGCATACTTGATTCACTTAGTATCATGTTTTTTTTAGCACTTGCAAACTGATTCTTCATGAAGCCTCAACTTATTCACCATGGCAGGGACCGAAGATTCGGACTGGTTTCCTGAAGGATGGGAAACCAATTCAACTGAAAGAAGGTCAAGAAGTGATCATAACTACGGATTATAGCATCAAGGGGGATGAGGAAATGATTTCGAGGAGTTATCAAAAGCTTGCTGTGGACATGAAGCCAGGAAATAATATTGTGTTTGGGTGGCACAATCACCCTCATCACAGTCTTGTCTTGTGA

mRNA sequence

ATGGCCAATATTGATATTGAGGGAATTCTTACCCCAAAACAAAAATTTGTACCTGCTTCTCGATCGTACATGAGTATCATCGAGAGACTCAAAATTTGTACGTACATGAGTATCATCAAGAGACTTGAGATAACAATCTCAGGACCGCCGTGCAGAATACTCAAATCATGCTTGATACTAAGCACTTGCAAACTGATTCTTCATGAAGCCTCAACTTATTCACCATGGCAGGGACCGAAGATTCGGACTGGTTTCCTGAAGGATGGGAAACCAATTCAACTGAAAGAAGGTCAAGAAGTGATCATAACTACGGATTATAGCATCAAGGGGGATGAGGAAATGATTTCGAGGAGTTATCAAAAGCTTGCTGTGGACATGAAGCCAGGAAATAATATTGTGTTTGGGTGGCACAATCACCCTCATCACAGTCTTGTCTTGTGA

Coding sequence (CDS)

ATGGCCAATATTGATATTGAGGGAATTCTTACCCCAAAACAAAAATTTGTACCTGCTTCTCGATCGTACATGAGTATCATCGAGAGACTCAAAATTTGTACGTACATGAGTATCATCAAGAGACTTGAGATAACAATCTCAGGACCGCCGTGCAGAATACTCAAATCATGCTTGATACTAAGCACTTGCAAACTGATTCTTCATGAAGCCTCAACTTATTCACCATGGCAGGGACCGAAGATTCGGACTGGTTTCCTGAAGGATGGGAAACCAATTCAACTGAAAGAAGGTCAAGAAGTGATCATAACTACGGATTATAGCATCAAGGGGGATGAGGAAATGATTTCGAGGAGTTATCAAAAGCTTGCTGTGGACATGAAGCCAGGAAATAATATTGTGTTTGGGTGGCACAATCACCCTCATCACAGTCTTGTCTTGTGA

Protein sequence

MANIDIEGILTPKQKFVPASRSYMSIIERLKICTYMSIIKRLEITISGPPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISRSYQKLAVDMKPGNNIVFGWHNHPHHSLVL

Homology

[Close]

BLAST of HG10008044.1 vs. NCBI nr
Match: AKN59232.1 (pyruvate kinase [Cucumis sativus])

HSP 1 Score: 115.9 bits (289), Expect = 2.9e-22
Identity = 74/145 (51.03%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           MANIDIEGIL         PK K V    PASRS   I + L+    ++       T   
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
               +      +   +++    +     +GP+IRTGFLKDGKPIQLKEGQEVIITTDYSI
Sbjct: 61  HQETLNNLRTAMQNTQIL---CAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDEEMIS SYQKLAVD+KPGNNI+
Sbjct: 121 KGDEEMISMSYQKLAVDLKPGNNIL 142

[Close]

BLAST of HG10008044.1 vs. NCBI nr
Match: XP_008445909.1 (PREDICTED: pyruvate kinase, cytosolic isozyme [Cucumis melo] >KAA0034093.1 pyruvate kinase, cytosolic isozyme [Cucumis melo var. makuwa] >TYK15825.1 pyruvate kinase, cytosolic isozyme [Cucumis melo var. makuwa])

HSP 1 Score: 115.9 bits (289), Expect = 2.9e-22
Identity = 74/145 (51.03%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           MANIDIEGIL         PK K V    PASRS   I + L+    ++       T   
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
               +      +   +++    +     +GP+IRTGFLKDGKPIQLKEGQEVIITTDYSI
Sbjct: 61  HQETLNNLRTAMQNTQIL---CAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDEEMIS SYQKLAVD+KPGNNI+
Sbjct: 121 KGDEEMISMSYQKLAVDLKPGNNIL 142

[Close]

BLAST of HG10008044.1 vs. NCBI nr
Match: XP_004147096.1 (pyruvate kinase, cytosolic isozyme [Cucumis sativus] >XP_031741666.1 pyruvate kinase, cytosolic isozyme [Cucumis sativus] >KGN51576.1 hypothetical protein Csa_008390 [Cucumis sativus])

HSP 1 Score: 115.9 bits (289), Expect = 2.9e-22
Identity = 74/145 (51.03%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           MANIDIEGIL         PK K V    PASRS   I + L+    ++       T   
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
               +      +   +++    +     +GP+IRTGFLKDGKPIQLKEGQEVIITTDYSI
Sbjct: 61  HQETLNNLRTAMQNTQIL---CAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDEEMIS SYQKLAVD+KPGNNI+
Sbjct: 121 KGDEEMISMSYQKLAVDLKPGNNIL 142

[Close]

BLAST of HG10008044.1 vs. NCBI nr
Match: XP_038892449.1 (pyruvate kinase, cytosolic isozyme [Benincasa hispida] >XP_038892452.1 pyruvate kinase, cytosolic isozyme [Benincasa hispida])

HSP 1 Score: 113.6 bits (283), Expect = 1.4e-21
Identity = 73/145 (50.34%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           MANIDIEGIL         PK K V    PASRS   I + L+    ++       T   
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
               +      +   +++    +     +GP+IRTGFLKDGKPIQLKEGQEVIITTDYSI
Sbjct: 61  HQETLNNLRTAMQNTQIL---CAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDEEMIS SYQKLAVD+KPGN+I+
Sbjct: 121 KGDEEMISMSYQKLAVDLKPGNSIL 142

[Close]

BLAST of HG10008044.1 vs. NCBI nr
Match: XP_022945152.1 (pyruvate kinase, cytosolic isozyme [Cucurbita moschata] >KAG6573764.1 hypothetical protein SDJN03_27651, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012837.1 hypothetical protein SDJN02_25590 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 112.1 bits (279), Expect = 4.1e-21
Identity = 71/145 (48.97%), Postives = 87/145 (60.00%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           MANIDIEGIL         PK K V    PASRS   I + LK    ++       T   
Sbjct: 1   MANIDIEGILKELPNDGRVPKTKIVCTLGPASRSVAMIEKLLKAGMNVARFNFSHGTHEY 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
               +      +   +++    +     +GP+IRTGFLKDGKP+QLKEG+EV ITTDYSI
Sbjct: 61  HQETLDNLRTAMQNTQIL---CAVMLDTKGPEIRTGFLKDGKPVQLKEGEEVTITTDYSI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDE+MIS SYQKLAVD+KPGNNI+
Sbjct: 121 KGDEKMISMSYQKLAVDLKPGNNIL 142

[Close]

BLAST of HG10008044.1 vs. ExPASy Swiss-Prot
Match: Q42806 (Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 9.6e-21
Identity = 66/157 (42.04%), Postives = 87/157 (55.41%), Query Frame = 0


Query: 1   MANIDIEGILTPKQKF---VPASRSYMSIIERLKICTYMSIIKRLEITISGPPCRILKSC 60
           MANIDIEGIL  +Q +   VP ++          +CT     + +E+T      ++L++ 
Sbjct: 1   MANIDIEGILKQQQPYDGRVPKTKI---------VCTLGPASRSVEMTE-----KLLRAG 60

Query: 61  L-----------------ILSTCKLILHE----ASTYSPWQGPKIRTGFLKDGKPIQLKE 120
           +                  L+  K  +H      +     +GP+IRTGFLKDGKPIQLKE
Sbjct: 61  MNVARFNFSHGTHDYHQETLNNLKTAMHNTGILCAVMLDTKGPEIRTGFLKDGKPIQLKE 120

Query: 121 GQEVIITTDYSIKGDEEMISRSYQKLAVDMKPGNNIV 134
           GQEV ITTDY IKGD EMIS SY+KL V +KPGN I+
Sbjct: 121 GQEVTITTDYDIKGDPEMISMSYKKLPVHLKPGNTIL 143

[Close]

BLAST of HG10008044.1 vs. ExPASy Swiss-Prot
Match: P22200 (Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 6.9e-19
Identity = 41/57 (71.93%), Postives = 52/57 (91.23%), Query Frame = 0


Query: 77  QGPKIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISRSYQKLAVDMKPGNNIV 134
           +GP+IRTGFL DGKPIQLKEGQE+ ++TDY+IKG+EEMIS SY+KL +D+KPGN I+
Sbjct: 86  KGPEIRTGFLTDGKPIQLKEGQEITVSTDYTIKGNEEMISMSYKKLVMDLKPGNTIL 142

[Close]

BLAST of HG10008044.1 vs. ExPASy Swiss-Prot
Match: Q42954 (Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.9e-16
Identity = 39/57 (68.42%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 77  QGPKIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISRSYQKLAVDMKPGNNIV 134
           +GP+IRTGFLKD KP+QLK+GQE+ I+TDYSIKGDE MI  SY+KLA D+KP + I+
Sbjct: 84  KGPEIRTGFLKDAKPVQLKQGQEITISTDYSIKGDESMICMSYKKLAEDVKPQSVIL 140

[Close]

BLAST of HG10008044.1 vs. ExPASy Swiss-Prot
Match: O65595 (Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g26390 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.4e-16
Identity = 37/53 (69.81%), Postives = 46/53 (86.79%), Query Frame = 0


Query: 77  QGPKIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISRSYQKLAVDMKPG 130
           +GP+IRTGFLKDGKPIQLK+GQE+ I+TDY +KGDE+ I  SY+KLA D+ PG
Sbjct: 73  KGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQDVNPG 125

[Close]

BLAST of HG10008044.1 vs. ExPASy Swiss-Prot
Match: O44006 (Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 8.4e-09
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 0


Query: 77  QGPKIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISRSYQKLAVDMKPGNNIV 134
           +GP+IRTG L+  KPI+L  G  + I TDYS  G++  I+ SY+KL   +KPGN I+
Sbjct: 123 KGPEIRTGMLEGDKPIELHAGDMLKIVTDYSFVGNKSCIACSYEKLPSSVKPGNTIL 179

[Close]

BLAST of HG10008044.1 vs. ExPASy TrEMBL
Match: A0A5A7SUF8 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003300 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-22
Identity = 74/145 (51.03%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           MANIDIEGIL         PK K V    PASRS   I + L+    ++       T   
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
               +      +   +++    +     +GP+IRTGFLKDGKPIQLKEGQEVIITTDYSI
Sbjct: 61  HQETLNNLRTAMQNTQIL---CAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDEEMIS SYQKLAVD+KPGNNI+
Sbjct: 121 KGDEEMISMSYQKLAVDLKPGNNIL 142

[Close]

BLAST of HG10008044.1 vs. ExPASy TrEMBL
Match: A0A0A0KV32 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_5G580610 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-22
Identity = 74/145 (51.03%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           MANIDIEGIL         PK K V    PASRS   I + L+    ++       T   
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
               +      +   +++    +     +GP+IRTGFLKDGKPIQLKEGQEVIITTDYSI
Sbjct: 61  HQETLNNLRTAMQNTQIL---CAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDEEMIS SYQKLAVD+KPGNNI+
Sbjct: 121 KGDEEMISMSYQKLAVDLKPGNNIL 142

[Close]

BLAST of HG10008044.1 vs. ExPASy TrEMBL
Match: A0A218KBQ1 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=PK32 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-22
Identity = 74/145 (51.03%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           MANIDIEGIL         PK K V    PASRS   I + L+    ++       T   
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
               +      +   +++    +     +GP+IRTGFLKDGKPIQLKEGQEVIITTDYSI
Sbjct: 61  HQETLNNLRTAMQNTQIL---CAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDEEMIS SYQKLAVD+KPGNNI+
Sbjct: 121 KGDEEMISMSYQKLAVDLKPGNNIL 142

[Close]

BLAST of HG10008044.1 vs. ExPASy TrEMBL
Match: A0A1S3BDT2 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103488792 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-22
Identity = 74/145 (51.03%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           MANIDIEGIL         PK K V    PASRS   I + L+    ++       T   
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
               +      +   +++    +     +GP+IRTGFLKDGKPIQLKEGQEVIITTDYSI
Sbjct: 61  HQETLNNLRTAMQNTQIL---CAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDEEMIS SYQKLAVD+KPGNNI+
Sbjct: 121 KGDEEMISMSYQKLAVDLKPGNNIL 142

[Close]

BLAST of HG10008044.1 vs. ExPASy TrEMBL
Match: A0A6J1G050 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111449475 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 2.0e-21
Identity = 71/145 (48.97%), Postives = 87/145 (60.00%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           MANIDIEGIL         PK K V    PASRS   I + LK    ++       T   
Sbjct: 1   MANIDIEGILKELPNDGRVPKTKIVCTLGPASRSVAMIEKLLKAGMNVARFNFSHGTHEY 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
               +      +   +++    +     +GP+IRTGFLKDGKP+QLKEG+EV ITTDYSI
Sbjct: 61  HQETLDNLRTAMQNTQIL---CAVMLDTKGPEIRTGFLKDGKPVQLKEGEEVTITTDYSI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDE+MIS SYQKLAVD+KPGNNI+
Sbjct: 121 KGDEKMISMSYQKLAVDLKPGNNIL 142

[Close]

BLAST of HG10008044.1 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 102.1 bits (253), Expect = 4.0e-22
Identity = 67/145 (46.21%), Postives = 82/145 (56.55%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           M+NIDIEGIL        TPK K V    PASRS   I + LK       + R   +   
Sbjct: 1   MSNIDIEGILKELPNDGRTPKTKIVCTLGPASRSVTMIEKLLKAGMN---VARFNFSHGS 60

Query: 61  PPCRILKSCLILSTCKLILHEASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITTDYSI 120
                     + +  +     A+     +GP+IRTGFLKDG PIQLKEGQE+ ITTDY I
Sbjct: 61  HEYHQETLDNLRTAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDI 120

Query: 121 KGDEEMISRSYQKLAVDMKPGNNIV 134
           KGDE+ IS SY+KL VD+KPGN I+
Sbjct: 121 KGDEKTISMSYKKLPVDVKPGNTIL 142

[Close]

BLAST of HG10008044.1 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 98.6 bits (244), Expect = 4.4e-21
Identity = 67/149 (44.97%), Postives = 88/149 (59.06%), Query Frame = 0


Query: 1   MANIDIEGIL--------TPKQKFV----PASRSYMSIIERLKICTYMSIIKRLEITISG 60
           M+NIDIEGIL         PK K V    PASR+ +S+IE+L +   M++ +      S 
Sbjct: 1   MSNIDIEGILKELPNDGRIPKTKIVCTLGPASRT-VSMIEKL-LKAGMNVAR---FNFSH 60

Query: 61  PPCRILKSCLILSTCKLILHE----ASTYSPWQGPKIRTGFLKDGKPIQLKEGQEVIITT 120
                 +    L   +  +H     A+     +GP+IRTGFLKDG PIQLKEGQE+ ITT
Sbjct: 61  GSHEYHQE--TLDNLRSAMHNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT 120

Query: 121 DYSIKGDEEMISRSYQKLAVDMKPGNNIV 134
           DY I+GDE  IS SY+KL +D+KPGN I+
Sbjct: 121 DYDIQGDESTISMSYKKLPLDVKPGNTIL 142

[Close]

BLAST of HG10008044.1 vs. TAIR 10
Match: AT5G56350.1 (Pyruvate kinase family protein )

HSP 1 Score: 87.4 bits (215), Expect = 1.0e-17
Identity = 38/53 (71.70%), Postives = 46/53 (86.79%), Query Frame = 0


Query: 77  QGPKIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISRSYQKLAVDMKPG 130
           +GP+IRTGFLKDGKPIQLK+GQE+ I+TDY +KGDE  I  SY+KLAVD+ PG
Sbjct: 74  KGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDENTICMSYKKLAVDVNPG 126

[Close]

BLAST of HG10008044.1 vs. TAIR 10
Match: AT4G26390.1 (Pyruvate kinase family protein )

HSP 1 Score: 85.5 bits (210), Expect = 3.9e-17
Identity = 37/53 (69.81%), Postives = 46/53 (86.79%), Query Frame = 0


Query: 77  QGPKIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISRSYQKLAVDMKPG 130
           +GP+IRTGFLKDGKPIQLK+GQE+ I+TDY +KGDE+ I  SY+KLA D+ PG
Sbjct: 73  KGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQDVNPG 125

[Close]

BLAST of HG10008044.1 vs. TAIR 10
Match: AT3G55810.1 (Pyruvate kinase family protein )

HSP 1 Score: 82.4 bits (202), Expect = 3.3e-16
Identity = 36/57 (63.16%), Postives = 48/57 (84.21%), Query Frame = 0


Query: 77  QGPKIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISRSYQKLAVDMKPGNNIV 134
           +GP+IRTGFLK+GKPIQL +GQE+ I+ DY I+GD  +IS SY+KLA D+KPG+ I+
Sbjct: 64  KGPEIRTGFLKEGKPIQLNQGQEITISIDYKIEGDSNIISMSYKKLAEDVKPGDVIL 120

The following BLAST results are available for this feature:

BLAST of HG10008044.1 vs. NCBI nr


Match Name	E-value	Identity	Description
AKN59232.1	2.9e-22	51.03	pyruvate kinase [Cucumis sativus]	[more]
XP_008445909.1	2.9e-22	51.03	PREDICTED: pyruvate kinase, cytosolic isozyme [Cucumis melo] >KAA0034093.1 pyruv...	[more]
XP_004147096.1	2.9e-22	51.03	pyruvate kinase, cytosolic isozyme [Cucumis sativus] >XP_031741666.1 pyruvate ki...	[more]
XP_038892449.1	1.4e-21	50.34	pyruvate kinase, cytosolic isozyme [Benincasa hispida] >XP_038892452.1 pyruvate ...	[more]
XP_022945152.1	4.1e-21	48.97	pyruvate kinase, cytosolic isozyme [Cucurbita moschata] >KAG6573764.1 hypothetic...	[more]

BLAST of HG10008044.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42806	9.6e-21	42.04	Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1	[more]
P22200	6.9e-19	71.93	Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q42954	1.9e-16	68.42	Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O65595	5.4e-16	69.81	Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
O44006	8.4e-09	49.12	Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1	[more]

BLAST of HG10008044.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SUF8	1.4e-22	51.03	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00330...	[more]
A0A0A0KV32	1.4e-22	51.03	Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_5G580610 PE=3 SV=1	[more]
A0A218KBQ1	1.4e-22	51.03	Pyruvate kinase OS=Cucumis sativus OX=3659 GN=PK32 PE=2 SV=1	[more]
A0A1S3BDT2	1.4e-22	51.03	Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103488792 PE=3 SV=1	[more]
A0A6J1G050	2.0e-21	48.97	Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111449475 PE=3 SV=1	[more]

BLAST of HG10008044.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G63680.1	4.0e-22	46.21	Pyruvate kinase family protein	[more]
AT5G08570.1	4.4e-21	44.97	Pyruvate kinase family protein	[more]
AT5G56350.1	1.0e-17	71.70	Pyruvate kinase family protein	[more]
AT4G26390.1	3.9e-17	69.81	Pyruvate kinase family protein	[more]
AT3G55810.1	3.3e-16	63.16	Pyruvate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 77..133 e-value: 1.2E-9 score: 37.6
IPR015806	Pyruvate kinase, insert domain superfamily	GENE3D	2.40.33.10		coord: 79..135 e-value: 1.5E-12 score: 49.2
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 75..133
None	No IPR available	PANTHER	PTHR11817:SF87	PYRUVATE KINASE	coord: 75..133
IPR011037	Pyruvate kinase-like, insert domain superfamily	SUPERFAMILY	50800	PK beta-barrel domain-like	coord: 79..134

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10008044	HG10008044	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10008044.1-cds	HG10008044.1-cds-Chr10:19044856..19045037	CDS
HG10008044.1-cds	HG10008044.1-cds-Chr10:19045850..19046108	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10008044.1	HG10008044.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030955	potassium ion binding
molecular_function	GO:0004743	pyruvate kinase activity