HG10006230.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10006230.1 vs. NCBI nr
Match: XP_038891172.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 950.7 bits (2456), Expect = 5.5e-273
Identity = 474/530 (89.43%), Postives = 503/530 (94.91%), Query Frame = 0

Query: 1   MAAFLLFFVMSM-LHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTA 60
           MAAFLLFF++SM LHFQL V LSSNTNPN+DVRYM  V D SELP+KEEYDYIIIGGGTA
Sbjct: 7   MAAFLLFFLVSMLLHFQLGVSLSSNTNPNEDVRYMKFVHDASELPKKEEYDYIIIGGGTA 66

Query: 61  GCPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVEN 120
           GCPLAATLSSNFSVLLLERGSEPTKYP+VLSEQ+F NVFT+EDDGKNPFQRFVSEDGVEN
Sbjct: 67  GCPLAATLSSNFSVLLLERGSEPTKYPTVLSEQTFTNVFTIEDDGKNPFQRFVSEDGVEN 126

Query: 121 LRGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQY 180
           LRGRVLGGTSMLNGGFYSRG+ EFFETAGVNWDMKLVEKAYEWVE+SVVFQASLNNGWQY
Sbjct: 127 LRGRVLGGTSMLNGGFYSRGHREFFETAGVNWDMKLVEKAYEWVEESVVFQASLNNGWQY 186

Query: 181 AFRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQAT 240
           AFRKGLLEAGVGPDNGFDL H VGTKIGGSIFDK+GNRHGAVELLN+AEPNNLKVAIQAT
Sbjct: 187 AFRKGLLEAGVGPDNGFDLRHSVGTKIGGSIFDKEGNRHGAVELLNKAEPNNLKVAIQAT 246

Query: 241 VEKILFSGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSS 300
           V+KILFSGLSASGV YSDSKGK H A +RKKGEIILSAGAIGSPQLLLLSGVGPKSHLSS
Sbjct: 247 VQKILFSGLSASGVSYSDSKGKLHRAFVRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSS 306

Query: 301 LKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLPFSM 360
           LK+P+VL+Q HVGQ MSDNPR+T NI+LP+PLV TAVKVVGTLENNIHLQSITSYLPFS+
Sbjct: 307 LKVPVVLNQHHVGQFMSDNPRYTVNILLPFPLVTTAVKVVGTLENNIHLQSITSYLPFSV 366

Query: 361 PPSFSLVPSGSDSVNLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPDDLA 420
           P SFSL+P G DSVNLSLATI GKFSEVLS+G L L +STDVKKSPTVRFNYYSHPDDLA
Sbjct: 367 PLSFSLIPHGFDSVNLSLATIAGKFSEVLSDGYLHL-TSTDVKKSPTVRFNYYSHPDDLA 426

Query: 421 KCVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTVTTY 480
           KCVRGVRK+GDFLKTQT+E IKIQDFEGRKGFEF+GPRLP+NLSDVSSVEEYCKKTVTTY
Sbjct: 427 KCVRGVRKIGDFLKTQTIENIKIQDFEGRKGFEFLGPRLPQNLSDVSSVEEYCKKTVTTY 486

Query: 481 WHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           WHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFS+SPGTNPMATL+MLGR
Sbjct: 487 WHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSESPGTNPMATLLMLGR 535

BLAST of HG10006230.1 vs. NCBI nr
Match: XP_008451933.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 932.2 bits (2408), Expect = 2.0e-267
Identity = 455/529 (86.01%), Postives = 495/529 (93.57%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAG 60
           MA+F LF ++SMLHF L  PLSSNT+PN+D RYM  V D S+LP +EEYDYIIIGGGTAG
Sbjct: 20  MASFPLFILISMLHFHLGFPLSSNTSPNEDFRYMKFVHDASDLPTEEEYDYIIIGGGTAG 79

Query: 61  CPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENL 120
           CPLAATLSSNFSVLLLERGSEPTKYPSVL EQSFLNV+TVEDDG+NPFQRFVSEDGVENL
Sbjct: 80  CPLAATLSSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENL 139

Query: 121 RGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYA 180
           RGRVLGGTSMLNGGFYSRG+ EFFETAGV WDM+LV+KAYEWVE+ VVF+ASLNNGWQYA
Sbjct: 140 RGRVLGGTSMLNGGFYSRGHREFFETAGVKWDMELVKKAYEWVEEGVVFEASLNNGWQYA 199

Query: 181 FRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATV 240
           FR GLLEAGVGP N FDLNHR+GTKIGGSIFDK+GNRHG+VELLN+A PNNLKV ++ATV
Sbjct: 200 FRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDKEGNRHGSVELLNKAHPNNLKVVVRATV 259

Query: 241 EKILFSGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 300
           EKI+FSGLSA GVLYSDSKGK HTASIR+KGEIILSAGAIGSPQLLLLSGVGPKSHLSSL
Sbjct: 260 EKIIFSGLSARGVLYSDSKGKLHTASIRRKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 319

Query: 301 KLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLPFSMP 360
           KLPIVLHQPHVGQSMSDNPRFT N+VLPYPLV+TAVKVVGTL++NIHLQSIT +LPFS+P
Sbjct: 320 KLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLP 379

Query: 361 PSFSLVPSGSDSVNLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPDDLAK 420
           PSFSL+P G DSVNLSLAT+VGKFSEVLSEGSL+LNSSTDVKK+P VRFNYYSHPDDLAK
Sbjct: 380 PSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAK 439

Query: 421 CVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTVTTYW 480
           CVRGVRKMGDFLKT T+EKIKIQDFEG++ F F+ P LPENLSD+  VE+YCKKTVTTYW
Sbjct: 440 CVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAFLEPPLPENLSDIGVVEKYCKKTVTTYW 499

Query: 481 HYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           HYHGGCLVGKVVDGNYRV+G+KNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 500 HYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFSDSPGTNPMATLMMLGR 548

BLAST of HG10006230.1 vs. NCBI nr
Match: XP_031736419.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus])

HSP 1 Score: 925.2 bits (2390), Expect = 2.5e-265
Identity = 453/529 (85.63%), Postives = 495/529 (93.57%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAG 60
           MA+FLLF ++SML FQL V LSSNT PN+D RYM  V D S+LP KE+YDYIIIGGGTAG
Sbjct: 7   MASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAG 66

Query: 61  CPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENL 120
           CPLAATLSSNFSVLLLERGSEPTKYPSVL EQSFLNV+TVEDDG+NPFQRFVSEDGVENL
Sbjct: 67  CPLAATLSSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENL 126

Query: 121 RGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYA 180
           RGRVLGGTSMLNGGFYSRG+ EFFETAGV WDM+LV+KAYEWVE+SVVF+ASLNNGWQYA
Sbjct: 127 RGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYA 186

Query: 181 FRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATV 240
           FR GLLEAGVGP NGF+LNHR+GTKIGGSIFDK+GNRHG+VELLN+A+PNNLKV ++ATV
Sbjct: 187 FRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATV 246

Query: 241 EKILFSGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 300
           EKI+FSGLSASGVLYSDSKG+ HTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL
Sbjct: 247 EKIIFSGLSASGVLYSDSKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 306

Query: 301 KLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLPFSMP 360
           KLP+VLHQPHVGQSMSDNPRFT NIVLPYPLV+TAVKVVGTL++NIHLQSIT +LPFS+P
Sbjct: 307 KLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLP 366

Query: 361 PSFSLVPSGSDSVNLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPDDLAK 420
           PSFSL+P   +SVNLSLATIVGKFSEV SEGSL+LNSSTDVKK+P VRFNYYSHPDDLAK
Sbjct: 367 PSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAK 426

Query: 421 CVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTVTTYW 480
           CVRGVRK GDFLKT T+EKIKIQDFEG++ F F+ P +PENLSD   VE++CK+TVTTYW
Sbjct: 427 CVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKETVTTYW 486

Query: 481 HYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           HYHGGCLVGKVVDGNYRV+GIKNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 487 HYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 535

BLAST of HG10006230.1 vs. NCBI nr
Match: XP_031736342.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KAE8651659.1 hypothetical protein Csa_021315 [Cucumis sativus])

HSP 1 Score: 924.1 bits (2387), Expect = 5.5e-265
Identity = 452/529 (85.44%), Postives = 495/529 (93.57%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAG 60
           MA+FLLF ++SML FQL V LSSNT PN+D RYM  V D S+LP KE+YDYIIIGGGTAG
Sbjct: 7   MASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAG 66

Query: 61  CPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENL 120
           CPLAATL+SNFSVLLLERGSEPTKYPSVL EQSFLNV+TVEDDG+NPFQRFVSEDGVENL
Sbjct: 67  CPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENL 126

Query: 121 RGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYA 180
           RGRVLGGTSMLNGGFYSRG+ EFFETAGV WDM+LV+KAYEWVE+SVVF+ASLNNGWQYA
Sbjct: 127 RGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYA 186

Query: 181 FRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATV 240
           FR GLLEAGVGP NGF+LNHR+GTKIGGSIFDK+GNRHG+VELLN+A+PNNLKV ++ATV
Sbjct: 187 FRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATV 246

Query: 241 EKILFSGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 300
           EKI+FSGLSASGVLYSDSKG+ HTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL
Sbjct: 247 EKIIFSGLSASGVLYSDSKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 306

Query: 301 KLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLPFSMP 360
           KLP+VLHQPHVGQSMSDNPRFT NIVLPYPLV+TAVKVVGTL++NIHLQSIT +LPFS+P
Sbjct: 307 KLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLP 366

Query: 361 PSFSLVPSGSDSVNLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPDDLAK 420
           PSFSL+P   +SVNLSLATIVGKFSEV SEGSL+LNSSTDVKK+P VRFNYYSHPDDLAK
Sbjct: 367 PSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAK 426

Query: 421 CVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTVTTYW 480
           CVRGVRK GDFLKT T+EKIKIQDFEG++ F F+ P +PENLSD   VE++CK+TVTTYW
Sbjct: 427 CVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKETVTTYW 486

Query: 481 HYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           HYHGGCLVGKVVDGNYRV+GIKNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 487 HYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 535

BLAST of HG10006230.1 vs. NCBI nr
Match: KAA0067300.1 ((R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10911.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 890.2 bits (2299), Expect = 8.8e-255
Identity = 433/496 (87.30%), Postives = 468/496 (94.35%), Query Frame = 0

Query: 34  MNLVQDGSELPEKEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLSEQS 93
           M  V D S+LP +EEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVL EQS
Sbjct: 1   MKFVHDASDLPTEEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQS 60

Query: 94  FLNVFTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDM 153
           FLNV+TVEDDG+NPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRG+ EFFETAGV WDM
Sbjct: 61  FLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVKWDM 120

Query: 154 KLVEKAYEWVEQSVVFQASLNNGWQYAFRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDK 213
           +LV+KAYEWVE+ VVF+ASLNNGWQYAFR GLLEAGVGP N FDLNHR+GTKIGGSIFDK
Sbjct: 121 ELVKKAYEWVEEGVVFEASLNNGWQYAFRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDK 180

Query: 214 KGNRHGAVELLNEAEPNNLKVAIQATVEKILFSGLSASGVLYSDSKGKFHTASIRKKGEI 273
           +GNRHG+VELLN+A PNNLKV ++ATVEKI+FSGLSA GVLYSDSKGK HTASIR+KGEI
Sbjct: 181 EGNRHGSVELLNKAHPNNLKVVVRATVEKIIFSGLSARGVLYSDSKGKLHTASIRRKGEI 240

Query: 274 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVV 333
           ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFT N+VLPYPLV+
Sbjct: 241 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVI 300

Query: 334 TAVKVVGTLENNIHLQSITSYLPFSMPPSFSLVPSGSDSVNLSLATIVGKFSEVLSEGSL 393
           TAVKVVGTL++NIHLQSIT +LPFS+PPSFSL+P G DSVNLSLAT+VGKFSEVLSEGSL
Sbjct: 301 TAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSL 360

Query: 394 QLNSSTDVKKSPTVRFNYYSHPDDLAKCVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEF 453
           +LNSSTDVKK+P VRFNYYSHPDDLAKCVRGVRKMGDFLKT T+EKIKIQDFEG++ F F
Sbjct: 361 KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAF 420

Query: 454 VGPRLPENLSDVSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFS 513
           + P LPENLSD+  VE+YCKKTVTTYWHYHGGCLVGKVVDGNYRV+G+KNLRVVDGSTFS
Sbjct: 421 LEPPLPENLSDIGVVEKYCKKTVTTYWHYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFS 480

Query: 514 DSPGTNPMATLMMLGR 530
           DSPGTNPMATLMMLGR
Sbjct: 481 DSPGTNPMATLMMLGR 496

BLAST of HG10006230.1 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.8e-149
Identity = 272/533 (51.03%), Postives = 367/533 (68.86%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAG 60
           M+A LL   + +L  Q +   S  T  N D  Y+    D ++L  +  YDY+I+GGGT+G
Sbjct: 6   MSAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSG 65

Query: 61  CPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENL 120
           CPLAATLS  + VL+LERGS PT YP+VL+   F+     EDDGK P +RFVSEDG++N+
Sbjct: 66  CPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 121 RGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYA 180
           RGRVLGGTSM+N G Y+R N   +  +GV+WDM LV K YEWVE ++VF+ +    WQ  
Sbjct: 126 RGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNY-QPWQSV 185

Query: 181 FRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATV 240
                LEAGV P++GF L+H  GT+I GS FD KG RH A ELLN+   NNL+V + A+V
Sbjct: 186 TGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASV 245

Query: 241 EKILFS---GLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHL 300
           EKI+FS   GL+A+GV+Y DS G  H A +R KGE+I+SAG IG+PQLLLLSGVGP+S+L
Sbjct: 246 EKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYL 305

Query: 301 SSLKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLPF 360
           SSL +P+VL  P+VGQ + DNPR   NI+ P P+  T V V+G + N+ +  S +S LPF
Sbjct: 306 SSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG-ISNDFYQCSFSS-LPF 365

Query: 361 SMPPSFSLVPSGSDSV-NLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPD 420
           + PP FS  PS S  + N + A    K +  LS GSL L SS++V+ SP V+FNYYS+P 
Sbjct: 366 TTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPT 425

Query: 421 DLAKCVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTV 480
           DL+ CV G++K+G+ L T  ++  K++D  G +GF  +G  LP++ +D ++ E +C+++V
Sbjct: 426 DLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESV 485

Query: 481 TTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
            +YWHYHGGCLVGKV+DG++RV GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 486 ASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGR 534

BLAST of HG10006230.1 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 270/534 (50.56%), Postives = 366/534 (68.54%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAG 60
           M+A LL   + +LH Q +   S     + D  Y++ V D ++   +  YDYII+GGGTAG
Sbjct: 6   MSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAG 65

Query: 61  CPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENL 120
           CPLAATLS+N+SVL+LERGS PT+YP++L    F+     EDDGK P +RFVSEDG++N+
Sbjct: 66  CPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 121 RGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYA 180
           RGRVLGGTSM+N G Y R N  FF   G+ WDM LV + YEWVE ++VF+   +  WQ  
Sbjct: 126 RGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPD-SQTWQTV 185

Query: 181 FRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATV 240
                LEAG+ P+NGF ++H  GT++ GS FD  G RH + ELLN+ +PNNL+VA+QA V
Sbjct: 186 IGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAV 245

Query: 241 EKILF----SGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSH 300
           EKI+F    SG++A GV+Y+DS G  H A +R +GE+ILSAG IGSPQLLLLSGVGP+S+
Sbjct: 246 EKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESY 305

Query: 301 LSSLKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLP 360
           L+SL + +V   P+VGQ + DNPR   NI+ P P+  + V V+G + ++ +  SI+S LP
Sbjct: 306 LTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLG-ITSDFYQCSISS-LP 365

Query: 361 FSMPPSFSLVPSGSDSV-NLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHP 420
           F  PP FS  P+ S  + N + A IV K    LS G++ LNSS+DV+  P V+FNYYS+ 
Sbjct: 366 FDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNL 425

Query: 421 DDLAKCVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKT 480
            DL+ CV G++K+G+ L T  +E  K++D  G  GF  +G  LPEN +D ++ E +C+++
Sbjct: 426 TDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRES 485

Query: 481 VTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           V +YWHYHGGCLVGKV+D  +RV GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 486 VASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGR 535

BLAST of HG10006230.1 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 267/533 (50.09%), Postives = 369/533 (69.23%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAG 60
           M+A LL   + +LH Q +   S  T  + D  Y++   D ++L  +  YDY+I+GGGT+G
Sbjct: 6   MSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSG 65

Query: 61  CPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENL 120
           CPLAATLS  + VL+LERGS PT YP+VL+   F+     EDDGK P +RFVSEDG++N+
Sbjct: 66  CPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 121 RGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYA 180
           RGRVLGGTS++N G Y+R N   +  +GV+WDM LV + YEWVE ++V++ + +  WQ  
Sbjct: 126 RGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPN-SQSWQSV 185

Query: 181 FRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATV 240
            +   LEAGV P++GF L+H  GT+I GS FD KG RH A ELLN+   NNL+V + A+V
Sbjct: 186 TKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASV 245

Query: 241 EKILFS---GLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHL 300
           EKI+FS   GL+A+GV+Y DS G  H A +R KGE+I+SAG IG+PQLLLLSGVGP+S+L
Sbjct: 246 EKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYL 305

Query: 301 SSLKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLPF 360
           SSL +P+VL  P+VGQ + DNPR   NI+ P P+  T V V+G + N+ +  S +S LPF
Sbjct: 306 SSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG-ISNDFYQCSFSS-LPF 365

Query: 361 SMPPSFSLVPSGSDSV-NLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPD 420
           + PP F   PS S  + N + A    K +  LS GSL L SS++V+ SP V+FNYYS+  
Sbjct: 366 TTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLT 425

Query: 421 DLAKCVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTV 480
           DL+ CV G++K+G+ L T  ++  K++D  G +GF  +G  LP++ +D ++ E +C+++V
Sbjct: 426 DLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESV 485

Query: 481 TTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
            +YWHYHGGCLVGKV+DG++RV GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 486 ASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGR 534

BLAST of HG10006230.1 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.2e-144
Identity = 265/535 (49.53%), Postives = 361/535 (67.48%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLA-VPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTA 60
           M+A +L   + +LH Q + V   +NT+   D  Y+  V +  +L  +  YDYII+GGGT+
Sbjct: 6   MSAVVLVLNLLVLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIVGGGTS 65

Query: 61  GCPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVEN 120
           GCPLAATLS+N+SVL+LERG+  T+YP+ L+   F      +DDGK P +RFVSEDG++N
Sbjct: 66  GCPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSEDGIDN 125

Query: 121 LRGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQY 180
           +R R+LGGT+++N G Y+R N  F+  +GV WD+ LV +AYEWVE ++V++ S N  WQ 
Sbjct: 126 VRSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPS-NQSWQS 185

Query: 181 AFRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQAT 240
                 LEAGV PDNGF L H  GT++ GS FD  G RH + ELLN+ +P+NLKVA++A 
Sbjct: 186 ITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVAVEAA 245

Query: 241 VEKILF----SGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKS 300
           V+KI+F    SGL+A GV+Y+DS G  H A +  KGE+ILSAG +G+PQLLLLSGVGP+S
Sbjct: 246 VQKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGVGPES 305

Query: 301 HLSSLKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYL 360
           +L+SL + +V   P+VGQ ++DNPR   NI+ P P+  + V V+G   +    Q   S L
Sbjct: 306 YLTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSD--FYQCSLSSL 365

Query: 361 PFSMPPSFSLVPSGSDSV-NLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSH 420
           PF  PP FSL P+ S  + N + A IV K    LS GSL L SS++V  +P V+FNY S 
Sbjct: 366 PFDTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFNYCSD 425

Query: 421 PDDLAKCVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKK 480
           P DL  CV G++K+G FL T  ++  K+ D  G  GF  +G  LPEN +D ++ E++C+ 
Sbjct: 426 PVDLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEKFCRD 485

Query: 481 TVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           TV +YWHYHGG +VGKV+DGN+RV GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 486 TVASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLMLGR 536

BLAST of HG10006230.1 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.1e-142
Identity = 262/534 (49.06%), Postives = 353/534 (66.10%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAG 60
           M+  L    + +LH Q +   S       D  Y+  V + ++   +  YDYI+IGGGT+G
Sbjct: 6   MSVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSG 65

Query: 61  CPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENL 120
           CPLAATLS  + VLLLERG+  T+YP+ L+   F      +DDGK P +RFVSEDG++N+
Sbjct: 66  CPLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSEDGIDNV 125

Query: 121 RGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYA 180
           R R+LGGT+++N G Y+R N  F+   G+ WD+ LV K YEWVE ++V + + N  WQ  
Sbjct: 126 RARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPN-NQSWQSV 185

Query: 181 FRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATV 240
             +G LEAG+ PDNGF L+H  GT++ GS FD  G RH A ELLN+ +PNNL VA+QA+V
Sbjct: 186 IGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASV 245

Query: 241 EKILF----SGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSH 300
           EKILF    S LSA GV+Y+DS G  H A +R  GE+I+SAG IG+PQLLLLSGVGP+S+
Sbjct: 246 EKILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESY 305

Query: 301 LSSLKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLP 360
           LSSL + +V   P+VGQ + +NPR   N   P P+  + V V+G   +  + Q   S LP
Sbjct: 306 LSSLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSLSSLP 365

Query: 361 FSMPPSFSLVPSGSDSV-NLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHP 420
           FS PP FSL P+ S  + N + A IV +    LS GS+ LNSS+DV+ +P ++FNYYS+ 
Sbjct: 366 FSTPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSNS 425

Query: 421 DDLAKCVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKT 480
            DLA CV G++K+GD L+T+ +E  K +D  G  GF ++G  LPEN +D +S E +C   
Sbjct: 426 TDLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETFCLDN 485

Query: 481 VTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           V +YWHYHGG LVGKV+D ++RV+GIK LRVVD STF   P ++P    +MLGR
Sbjct: 486 VASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGR 535

BLAST of HG10006230.1 vs. ExPASy TrEMBL
Match: A0A1S3BS25 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493083 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 9.8e-268
Identity = 455/529 (86.01%), Postives = 495/529 (93.57%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAG 60
           MA+F LF ++SMLHF L  PLSSNT+PN+D RYM  V D S+LP +EEYDYIIIGGGTAG
Sbjct: 20  MASFPLFILISMLHFHLGFPLSSNTSPNEDFRYMKFVHDASDLPTEEEYDYIIIGGGTAG 79

Query: 61  CPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENL 120
           CPLAATLSSNFSVLLLERGSEPTKYPSVL EQSFLNV+TVEDDG+NPFQRFVSEDGVENL
Sbjct: 80  CPLAATLSSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENL 139

Query: 121 RGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYA 180
           RGRVLGGTSMLNGGFYSRG+ EFFETAGV WDM+LV+KAYEWVE+ VVF+ASLNNGWQYA
Sbjct: 140 RGRVLGGTSMLNGGFYSRGHREFFETAGVKWDMELVKKAYEWVEEGVVFEASLNNGWQYA 199

Query: 181 FRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATV 240
           FR GLLEAGVGP N FDLNHR+GTKIGGSIFDK+GNRHG+VELLN+A PNNLKV ++ATV
Sbjct: 200 FRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDKEGNRHGSVELLNKAHPNNLKVVVRATV 259

Query: 241 EKILFSGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 300
           EKI+FSGLSA GVLYSDSKGK HTASIR+KGEIILSAGAIGSPQLLLLSGVGPKSHLSSL
Sbjct: 260 EKIIFSGLSARGVLYSDSKGKLHTASIRRKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 319

Query: 301 KLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLPFSMP 360
           KLPIVLHQPHVGQSMSDNPRFT N+VLPYPLV+TAVKVVGTL++NIHLQSIT +LPFS+P
Sbjct: 320 KLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLP 379

Query: 361 PSFSLVPSGSDSVNLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPDDLAK 420
           PSFSL+P G DSVNLSLAT+VGKFSEVLSEGSL+LNSSTDVKK+P VRFNYYSHPDDLAK
Sbjct: 380 PSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAK 439

Query: 421 CVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTVTTYW 480
           CVRGVRKMGDFLKT T+EKIKIQDFEG++ F F+ P LPENLSD+  VE+YCKKTVTTYW
Sbjct: 440 CVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAFLEPPLPENLSDIGVVEKYCKKTVTTYW 499

Query: 481 HYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           HYHGGCLVGKVVDGNYRV+G+KNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 500 HYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFSDSPGTNPMATLMMLGR 548

BLAST of HG10006230.1 vs. ExPASy TrEMBL
Match: A0A5A7VJA1 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00920 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 4.3e-255
Identity = 433/496 (87.30%), Postives = 468/496 (94.35%), Query Frame = 0

Query: 34  MNLVQDGSELPEKEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLSEQS 93
           M  V D S+LP +EEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVL EQS
Sbjct: 1   MKFVHDASDLPTEEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQS 60

Query: 94  FLNVFTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDM 153
           FLNV+TVEDDG+NPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRG+ EFFETAGV WDM
Sbjct: 61  FLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVKWDM 120

Query: 154 KLVEKAYEWVEQSVVFQASLNNGWQYAFRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDK 213
           +LV+KAYEWVE+ VVF+ASLNNGWQYAFR GLLEAGVGP N FDLNHR+GTKIGGSIFDK
Sbjct: 121 ELVKKAYEWVEEGVVFEASLNNGWQYAFRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDK 180

Query: 214 KGNRHGAVELLNEAEPNNLKVAIQATVEKILFSGLSASGVLYSDSKGKFHTASIRKKGEI 273
           +GNRHG+VELLN+A PNNLKV ++ATVEKI+FSGLSA GVLYSDSKGK HTASIR+KGEI
Sbjct: 181 EGNRHGSVELLNKAHPNNLKVVVRATVEKIIFSGLSARGVLYSDSKGKLHTASIRRKGEI 240

Query: 274 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVV 333
           ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFT N+VLPYPLV+
Sbjct: 241 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVI 300

Query: 334 TAVKVVGTLENNIHLQSITSYLPFSMPPSFSLVPSGSDSVNLSLATIVGKFSEVLSEGSL 393
           TAVKVVGTL++NIHLQSIT +LPFS+PPSFSL+P G DSVNLSLAT+VGKFSEVLSEGSL
Sbjct: 301 TAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSL 360

Query: 394 QLNSSTDVKKSPTVRFNYYSHPDDLAKCVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEF 453
           +LNSSTDVKK+P VRFNYYSHPDDLAKCVRGVRKMGDFLKT T+EKIKIQDFEG++ F F
Sbjct: 361 KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAF 420

Query: 454 VGPRLPENLSDVSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFS 513
           + P LPENLSD+  VE+YCKKTVTTYWHYHGGCLVGKVVDGNYRV+G+KNLRVVDGSTFS
Sbjct: 421 LEPPLPENLSDIGVVEKYCKKTVTTYWHYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFS 480

Query: 514 DSPGTNPMATLMMLGR 530
           DSPGTNPMATLMMLGR
Sbjct: 481 DSPGTNPMATLMMLGR 496

BLAST of HG10006230.1 vs. ExPASy TrEMBL
Match: A0A0A0LJ83 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055560 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.2e-252
Identity = 423/529 (79.96%), Postives = 480/529 (90.74%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAG 60
           MA+FLLF +MSMLHFQ   PLSSN+NPN+D++YMN VQD SEL E EEYDYI+IGGGTAG
Sbjct: 6   MASFLLFILMSMLHFQFGFPLSSNSNPNEDLKYMNFVQDASELLENEEYDYIVIGGGTAG 65

Query: 61  CPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENL 120
           CPLA TLS+NFSVLLLERG+ PTKYP+VLSEQ+F NVFT EDDG+NPFQRFVSEDGVE +
Sbjct: 66  CPLATTLSANFSVLLLERGNVPTKYPTVLSEQAFPNVFTTEDDGENPFQRFVSEDGVEII 125

Query: 121 RGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYA 180
           RGRVLGG+SMLN GFYSRG+PEFF+ +GV+WD +LVEKAYEWVE+SV+F+A++NNGWQYA
Sbjct: 126 RGRVLGGSSMLNAGFYSRGHPEFFDISGVDWDKELVEKAYEWVEESVIFEANINNGWQYA 185

Query: 181 FRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATV 240
           FRK LLEAGVGP +GFDLNHR+GTKIGGSIFDK+GNRHG+VELLN+A+PNNLKV IQA V
Sbjct: 186 FRKALLEAGVGPYHGFDLNHRIGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVGIQAVV 245

Query: 241 EKILFSGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 300
           +KILFS LSA+GV YSDSKG  HTASIRK GEII+SAG IGSPQLLLLSG+GPKSHL SL
Sbjct: 246 QKILFSDLSATGVSYSDSKGNLHTASIRKNGEIIVSAGTIGSPQLLLLSGIGPKSHLESL 305

Query: 301 KLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLPFSMP 360
           K+P+VLHQP+VGQSMSDNPR+T +++LPYP+  +AVK VGTLENN+HLQSIT +LPFS+P
Sbjct: 306 KIPVVLHQPYVGQSMSDNPRYTVHVILPYPMATSAVKAVGTLENNVHLQSITGFLPFSLP 365

Query: 361 PSFSLVPSGSDSVNLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPDDLAK 420
           PSFSLVP G DSVNLSLA++VGKFSEVLSEGSL L SSTDVK++P+V FNYYSHPDDLAK
Sbjct: 366 PSFSLVPPGLDSVNLSLASLVGKFSEVLSEGSLYLTSSTDVKENPSVSFNYYSHPDDLAK 425

Query: 421 CVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTVTTYW 480
           CVRGVRKMGD LKT+ ME IKIQDFEG K F F+ P LP NLSDV  VEE+CKKTVTTYW
Sbjct: 426 CVRGVRKMGDLLKTEAMENIKIQDFEGNKRFAFLEPSLPGNLSDVGLVEEFCKKTVTTYW 485

Query: 481 HYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           HYHGGCLVGKVVDGNY+VIGI+NLRVVDGSTF DSPGTNPMAT+MMLGR
Sbjct: 486 HYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFVDSPGTNPMATVMMLGR 534

BLAST of HG10006230.1 vs. ExPASy TrEMBL
Match: A0A1S3BTD3 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493053 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 9.8e-252
Identity = 426/529 (80.53%), Postives = 476/529 (89.98%), Query Frame = 0

Query: 1   MAAFLLFFVMSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAG 60
           MA+FLLF +MS LHFQ   PLSSNTN N+D +YMN VQD SELPE EEYDYI+IGGGTAG
Sbjct: 1   MASFLLFILMSTLHFQFGFPLSSNTNSNEDFKYMNFVQDASELPENEEYDYIVIGGGTAG 60

Query: 61  CPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENL 120
           CPLA TLSS FSVLLLERG+ PTKYP+VL E++F N FT+EDDG+NPFQRFVSEDGVE +
Sbjct: 61  CPLATTLSSTFSVLLLERGNVPTKYPTVLREETFPNAFTIEDDGENPFQRFVSEDGVEII 120

Query: 121 RGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYA 180
           RGRVLGG+SMLN GFYSRG+ EFF+ +GV+WDM+LVEKAYEWVE+SVVF+A++NNGWQ A
Sbjct: 121 RGRVLGGSSMLNAGFYSRGHQEFFDISGVDWDMELVEKAYEWVEESVVFEANINNGWQNA 180

Query: 181 FRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATV 240
           FRK LLEAGVGP NGFDLNHR+GTKIGGSIFDK+GNRHG++ELLN+A PNNLKVAIQATV
Sbjct: 181 FRKALLEAGVGPYNGFDLNHRIGTKIGGSIFDKEGNRHGSLELLNKAHPNNLKVAIQATV 240

Query: 241 EKILFSGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 300
           +KILFS LSA+GV YSDSKG  HTASIRK GEII+SAG IGSPQLLLLSG+GPKSHL SL
Sbjct: 241 QKILFSDLSATGVSYSDSKGNLHTASIRKNGEIIVSAGTIGSPQLLLLSGIGPKSHLESL 300

Query: 301 KLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLPFSMP 360
           +LP+VL QPHVGQSMSDNPR+  +++LPY L  +AVK VGTLENN+HLQSIT +LPFS+P
Sbjct: 301 ELPVVLDQPHVGQSMSDNPRYNVHVILPYQLATSAVKTVGTLENNVHLQSITGFLPFSLP 360

Query: 361 PSFSLVPSGSDSVNLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPDDLAK 420
           PSFSLVP G DSVNLSLA+IVGKFSEVLSEGSL L SSTDVKK+P+V FNYYSHPDDLAK
Sbjct: 361 PSFSLVPPGLDSVNLSLASIVGKFSEVLSEGSLYLTSSTDVKKNPSVSFNYYSHPDDLAK 420

Query: 421 CVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTVTTYW 480
           CVRGVRKMG+ LKT+ MEKIKIQDFEG K F FV P LPENLSDV SVEE+CKKTVTTYW
Sbjct: 421 CVRGVRKMGELLKTEAMEKIKIQDFEGNKRFAFVEPSLPENLSDVGSVEEFCKKTVTTYW 480

Query: 481 HYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           HYHGGCLVGKVVDGNY+VIGI+NLRVVDGSTF DSPGTNPMAT+MMLGR
Sbjct: 481 HYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFIDSPGTNPMATVMMLGR 529

BLAST of HG10006230.1 vs. ExPASy TrEMBL
Match: A0A5A7UQ82 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G001000 PE=3 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 7.8e-249
Identity = 420/520 (80.77%), Postives = 468/520 (90.00%), Query Frame = 0

Query: 10  MSMLHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAGCPLAATLSS 69
           MS LHFQ   PLSSNTN N+D +YMN VQD SELPE EEYDYI+IGGGTAGCPLA TLSS
Sbjct: 1   MSTLHFQFGFPLSSNTNSNEDFKYMNFVQDASELPENEEYDYIVIGGGTAGCPLATTLSS 60

Query: 70  NFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENLRGRVLGGTS 129
            FSVLLLERG+ PTKYP+VL E++F N FT+EDDG+NPFQRFVSEDGVE +RGRVLGG+S
Sbjct: 61  TFSVLLLERGNVPTKYPTVLREETFPNAFTIEDDGENPFQRFVSEDGVEIIRGRVLGGSS 120

Query: 130 MLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYAFRKGLLEAG 189
           MLN GFYSRG+ EFF+ +GV+WDM+LVEKAYEWVE+SVVF+A++NNGWQ AFRK LLEAG
Sbjct: 121 MLNAGFYSRGHQEFFDISGVDWDMELVEKAYEWVEESVVFEANINNGWQNAFRKALLEAG 180

Query: 190 VGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATVEKILFSGLS 249
           VGP NGFDLNHR+GTKIGGSIFDK+GNRHG++ELLN+A PNNLKVAIQATV+KILFS LS
Sbjct: 181 VGPYNGFDLNHRIGTKIGGSIFDKEGNRHGSLELLNKAHPNNLKVAIQATVQKILFSDLS 240

Query: 250 ASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQP 309
           A+GV YSDSKG  HTASIRK GEII+SAG IGSPQLLLLSG+GPKSHL SL+LP+VL QP
Sbjct: 241 ATGVSYSDSKGNLHTASIRKNGEIIVSAGTIGSPQLLLLSGIGPKSHLESLELPVVLDQP 300

Query: 310 HVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYLPFSMPPSFSLVPSG 369
           HVGQSMSDNPR+  +++LPY L  +AVK VGTLENN+HLQSIT +LPFS+PPSFSLVP G
Sbjct: 301 HVGQSMSDNPRYNVHVILPYQLATSAVKTVGTLENNVHLQSITGFLPFSLPPSFSLVPPG 360

Query: 370 SDSVNLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPDDLAKCVRGVRKMG 429
            DSVNLSLA+IVGKFSEVLSEGSL L SSTDVKK+P+V FNYYSHPDDLAKCVRGVRKMG
Sbjct: 361 LDSVNLSLASIVGKFSEVLSEGSLYLTSSTDVKKNPSVSFNYYSHPDDLAKCVRGVRKMG 420

Query: 430 DFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTVTTYWHYHGGCLVG 489
           + LKT+ MEKIKIQDFEG K F FV P LPENLSDV SVEE+CKKTVTTYWHYHGGCLVG
Sbjct: 421 ELLKTEAMEKIKIQDFEGNKRFAFVEPSLPENLSDVGSVEEFCKKTVTTYWHYHGGCLVG 480

Query: 490 KVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           KVVDGNY+VIGI+NLRVVDGSTF DSPGTNPMAT+MMLGR
Sbjct: 481 KVVDGNYKVIGIENLRVVDGSTFIDSPGTNPMATVMMLGR 520

BLAST of HG10006230.1 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 506.1 bits (1302), Expect = 3.3e-143
Identity = 255/534 (47.75%), Postives = 362/534 (67.79%), Query Frame = 0

Query: 6   LFFVMSMLHFQLAVPLSSNTNP--NQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAGCPL 65
           L +   ++   L V   SN  P  N+   +M  + + ++   ++ YDYII+GGGTAGCPL
Sbjct: 9   LLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPL 68

Query: 66  AATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENLRGR 125
           AATLS +F VLLLERG  P   P+V+S   FL   T  ++  +P Q F+SE+GV N RGR
Sbjct: 69  AATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGR 128

Query: 126 VLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYAFRK 185
           VLGG+S +N GFYSR + +FFE +G+ WD+  V ++YEWVE+++VF+  L   WQ A R 
Sbjct: 129 VLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRT-WQTAIRD 188

Query: 186 GLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATVEKI 245
            LLE GV P NGF L H+VGTKIGGS FD+ G RH + +LL  A  +N++VA+ ATVE++
Sbjct: 189 ALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERV 248

Query: 246 LF--------SGLSASGVLYSDSKGKFHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKS 305
           L         S +SA GV+Y D  G+FH A IR +GE+ILSAGA+GSPQLL LSG+GP+S
Sbjct: 249 LLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRS 308

Query: 306 HLSSLKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITSYL 365
           +LS+  +P+ L QPHVG  + DNPR   +IV P P+  + ++VVG  E+   L++ ++ +
Sbjct: 309 YLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVI 368

Query: 366 PFSMPPSFSLVPSGSDSVNLSLATIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHP 425
           PF+ P     + + +  + + + TI+ K    +S G L+L +STDV+ +P VRFNY+S P
Sbjct: 369 PFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDP 428

Query: 426 DDLAKCVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKT 485
            DL +CV G RK+G+ L+++ M+   I+++ G + F FVG  LP + S+   + ++C++T
Sbjct: 429 QDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRT 488

Query: 486 VTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           V+T WHYHGG +VGKVVD + +VIG+ +LR+VDGSTF+ SPGTNP ATLMMLGR
Sbjct: 489 VSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGR 540

BLAST of HG10006230.1 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 400.6 bits (1028), Expect = 2.0e-111
Identity = 244/569 (42.88%), Postives = 324/569 (56.94%), Query Frame = 0

Query: 6   LFFVMSM-LHFQLAVPLSSNTNPNQDVRYMNLVQDGSELPEKEEYDYIIIGGGTAGCPLA 65
           LF  +S+ LH  +    SS+  PN      + ++D +  P    YDYIIIGGGTAGCPLA
Sbjct: 10  LFVALSLFLHSPIC---SSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGGTAGCPLA 69

Query: 66  ATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDGKNPFQRFVSEDGVENLRGRV 125
           ATLS N SVLLLERG  P   P++    +F      +    +P QRFVSEDGV N R RV
Sbjct: 70  ATLSQNASVLLLERGDSPYNNPNITRLSAF-GAALSDLSESSPSQRFVSEDGVINARARV 129

Query: 126 LGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVEQSVVFQASLNNGWQYAFRKG 185
           LGG S LN GFY+R   ++    G  WD  L  ++Y+WVE  V FQ  +   WQ A R G
Sbjct: 130 LGGGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGR-WQTAVRDG 189

Query: 186 LLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELLNEAEPNNLKVAIQATVEKIL 245
           LLEAG+ P+NGF  +H  GTK GG+IFD+ GNRH A +LL  A+P  + V + ATV +IL
Sbjct: 190 LLEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRIL 249

Query: 246 FSGLS-----ASGVLYSDSKGKFHTASIRK--KGEIILSAGAIGSPQLLLLSGVGPKSHL 305
           F         A+GV+Y D  G+ H A +++    EIILSAG +GSPQLL+LSGVGP + L
Sbjct: 250 FRTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQL 309

Query: 306 SSLKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKVVGTLENNIHLQSITS---- 365
            +  + +V+ QPHVGQ M DNP     +  P P+ V+ ++VVG      ++++       
Sbjct: 310 QAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFG 369

Query: 366 ---------------YLPFSMPPSFSLVPSGSDSVNLSLAT------IVGKFSEVLSEGS 425
                          Y  FS  P  +L+ S S    LS A       ++ K    LS G 
Sbjct: 370 GGGGGSSGSSSTRDYYAMFS--PRATLLESNS-MTKLSSAQPFQGGFLLEKVMGPLSTGH 429

Query: 426 LQLNSSTDVKKSPTVRFNYYSHPDDLAKCVRGVRKMGDFLKTQTMEKIKIQDFEGRKGFE 485
           L+L  + + K +P V FNY+ HPDDL +CVRG++ +   ++++   + K  D      FE
Sbjct: 430 LEL-KTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV----SFE 489

Query: 486 FV------------GPRLPENLSDVSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNYRVIG 530
           ++             PR     S   S EE+C+ TVTT WHYHGGC+VG+VVDG+Y+VIG
Sbjct: 490 YLLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIG 549

BLAST of HG10006230.1 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 396.4 bits (1017), Expect = 3.7e-110
Identity = 230/532 (43.23%), Postives = 324/532 (60.90%), Query Frame = 0

Query: 37  VQDGSELPEKEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLN 96
           ++D +  P+   +DYIIIGGGTAGC LAATLS N SVL+LERG  P + P+     + +N
Sbjct: 61  MKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSVN 120

Query: 97  VFTVEDDGKNPF-QRFVSEDGVENLRGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKL 156
             T+ ++  N + Q F+SEDGV N R RVLGG S++NGGFYSR   ++ E A   W+M+ 
Sbjct: 121 --TLLNNTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEEA--EWEMEE 180

Query: 157 VEKAYEWVEQSVVFQASLNNGWQYAFRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKG 216
           VE AYEWVE+ +VF+  +   WQ AF+ GLLEAG  PDNGF  +H  GTKIGG+IFD+ G
Sbjct: 181 VEAAYEWVEKKLVFEPQVIE-WQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAG 240

Query: 217 NRHGAVELLNEAEPNNLKVAIQATVEKILFSGLSASGVLYSDSKGKFHTASIRKK--GEI 276
           +RH A  LL  A PN + V + A+V K+LF+   A  VL+ D+ G FH A++  K   E+
Sbjct: 241 HRHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAYEVLFEDANGVFHKANLANKATNEV 300

Query: 277 ILSAGAIGSPQLLLLSGVGPKSHLSSLKL-PIVLHQPHVGQSMSDNPRFTTNIVLPYPLV 336
           ILSAGA+GSPQLL+LSGVGP  HL +  + P+VL QP VGQ M+DNP     I  P P+ 
Sbjct: 301 ILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQPVE 360

Query: 337 VTAVKVVGTLENNIHLQSITSY-LPFSMPPSF-----SLVPSGSDSVNLSLAT------- 396
           ++ ++ VG  + + +++ ++   L F +   F     +L+   S + +  + T       
Sbjct: 361 LSLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAVLL 420

Query: 397 ---------------IVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPDDLAKCVRG 456
                          I  K     S+G ++L  +T+ + +P+V FNYY  P+DL KCV+G
Sbjct: 421 KSFDVKLEVRMNGGLIFQKVDGPASKGHMKLR-NTNPRDNPSVTFNYYQEPEDLNKCVKG 480

Query: 457 VRKMGDFLKTQTMEKIKIQDFEGRKGFEFVGPRL-------PENLSDVSSVEEYCKKTVT 516
           +  +   + ++   K K   + G    E +   L       P +++   +++++C  TVT
Sbjct: 481 LNTIIRMINSKAFSKYK---YPGVTARELLNLMLALPINLRPRHVTSAFNLKQFCIDTVT 540

Query: 517 TYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           + WHYHGGC VGKVVD NY+V+GI  LRV+DGSTF  SPGTNP AT+MMLGR
Sbjct: 541 SVWHYHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLGR 582

BLAST of HG10006230.1 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.4e-109
Identity = 229/525 (43.62%), Postives = 308/525 (58.67%), Query Frame = 0

Query: 45  EKEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLSEQSFLNVFTVEDDG 104
           +   YDYI+IGGGTAGCPLAATLS NFSVL+LERG  P    +V   ++F ++   +   
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNF-HIGLADISA 118

Query: 105 KNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGNPEFFETAGVNWDMKLVEKAYEWVE 164
            +  Q FVS DGV N R RVLGG S +N GFYSR +  F + AG  WD KLV+++Y WVE
Sbjct: 119 SSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVE 178

Query: 165 QSVVFQASLNNGWQYAFRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFDKKGNRHGAVELL 224
           + +V Q  L   WQ A R  LLE GV P NGF  +H  GTKIGG+IFD+ G RH A ELL
Sbjct: 179 REIVHQPKLTL-WQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELL 238

Query: 225 NEAEPNNLKVAIQATVEKILF--SGL--SASGVLYSDSKGKFHTA--SIRKKGEIILSAG 284
             A P  L+V I ATV+KI+F  SG     +GV++ D KG  H A  S RK  E+ILS+G
Sbjct: 239 AYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSG 298

Query: 285 AIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFTTNIVLPYPLVVTAVKV 344
           AIGSPQ+L+LSG+GPK  L  LK+P+VL   HVG+ M+DNP  T  +    P+  + ++ 
Sbjct: 299 AIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQT 358

Query: 345 VGTLENNIHLQSITSY------------LPFSMPPSFSLVPSGSDSVNLSLA-------- 404
           VG  +  +++++ T +            +  +    FS +P+       + A        
Sbjct: 359 VGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQ 418

Query: 405 --------TIVGKFSEVLSEGSLQLNSSTDVKKSPTVRFNYYSHPDDLAKCVRGVRKMGD 464
                    I+ K +  +S G L L  +T+V  +P+V FNY+ HP DL +CV  +R +  
Sbjct: 419 LHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSK 478

Query: 465 ------FLKTQTMEKIKIQDFEGRKGFEFVGPRLPENLSDVSSVEEYCKKTVTTYWHYHG 524
                 FL     +K  +           +  R P+ L+D  S+ ++CK TV T WHYHG
Sbjct: 479 VVTSNRFLNYTQCDKQNVHKMLSLSVKANINLR-PKQLNDTKSMAQFCKDTVVTIWHYHG 538

Query: 525 GCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 530
           GCLVGKVV  N +V+G+  LRV+DGSTF +SPGTNP AT+MM+GR
Sbjct: 539 GCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGR 577

BLAST of HG10006230.1 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 385.6 bits (989), Expect = 6.5e-107
Identity = 232/543 (42.73%), Postives = 312/543 (57.46%), Query Frame = 0

Query: 33  YMNLVQDGSELPEKEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLSEQ 92
           Y + ++D +  P    +DYIIIGGGT+GC LAATLS N SVL+LERG  P   P+    +
Sbjct: 30  YYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIE 89

Query: 93  SFLNVFTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGNPEFFETAGVNWD 152
           +F    +     K+  Q F+SEDGV N R RVLGG S+LN GFY+R   E+ +     W 
Sbjct: 90  NFATTLS-NTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKE--TEWK 149

Query: 153 MKLVEKAYEWVEQSVVFQASLNNGWQYAFRKGLLEAGVGPDNGFDLNHRVGTKIGGSIFD 212
              VE AYEWVE+ V FQ  +  GWQ AF+ GLLEAG  P NGF  +H  GTKIGG+IFD
Sbjct: 150 TDEVEAAYEWVEKKVAFQPPV-LGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFD 209

Query: 213 KKGNRHGAVELLNEAEPNNLKVAIQATVEKILFSGL-----SASGVLYSDSKGKFHTASI 272
           + G+RH A +LL  A P N+ V + A+V KILF+        A GV++ D+ G  H A +
Sbjct: 210 RAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAEL 269

Query: 273 RKK--GEIILSAGAIGSPQLLLLSGVGPKSHLSSLKL-PIVLHQPHVGQSMSDNPRFTTN 332
            K    E+ILSAGAIGSPQLL+LSG+GP +HL++  + P+VL  P VGQ M DNP     
Sbjct: 270 EKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIF 329

Query: 333 IVLPYPLVVTAVKVVGTLENNIHLQSI-------------------------TSYLPFSM 392
           I  P P+ V+ ++VVG  +   +++                           TS    + 
Sbjct: 330 IPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTT 389

Query: 393 PPSFSLVPSGSDSVN-----LSLAT----IVGKFSEVLSEGSLQLNSSTDVKKSPTVRFN 452
            P+ S   S +D  N     L+  T    I+ K +  +S G L+L  +T+   +P+VRFN
Sbjct: 390 SPTLS-TQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELR-NTNPDDNPSVRFN 449

Query: 453 YYSHPDDLAKCVRGVRKMGDFLKTQTMEKIKIQD--FEGRKGFEFVGPR--LPENLSDVS 512
           YY  P+DL  CV G+  +   + ++   K K  D    G        P    P +++ + 
Sbjct: 450 YYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMF 509

Query: 513 SVEEYCKKTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMM 530
           ++ ++C  TV T WHYHGGC VG+VVD NYRV+GI +LRV+DGSTF  SPGTNP AT+MM
Sbjct: 510 NLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMM 566