HG10005743.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10005743.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	cysteine proteinase RD19a-like
Location	Chr07: 5313313 .. 5316663 (-)
Sequence length	1146
RNA-Seq Expression	HG10005743.1
Synteny	HG10005743.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCGGAATTTCTTCTTGTTCGCCGTGATCGCCGCCACCTTCTGCTCATCGGAATCTTTGGCCTCACAGCATTCCGTCGAACGAAATGGCGATCCGCTGATTCGTCAAGTCGTCGACGATGGCGATTTCAATCGTCTTCCTCTCGGAGCGGAGCACCATTTTTCGCTCTTCAAGCGAAAGTTCGGGAAATCGTATGCCACTGAGGAAGAGCACGATCTTAGATTCAAGATCTTCAAGGCTAATATGCGACGAGCGCAGCTCCATCAGTCATTTGATCCTTCCGCCATTCACGGCGTCACTCAGTTCGCCGATTTGACCCCTTTCGAGTTTCGGAAGGCATTTCTAGGGCTCCGAGGTCACCGTCTCAGGCTTCCTGTTGATACTAACGCAGCTCCGATTCTTCCTACGGAGAATCTTCCGATTGATTTTGATTGGAGAGAACGTGGTGCTGTAACTCCGGTGAAAAATCAGGTATATATCTCAACTAAACTCTCCTGTTTAATTGCAATTTTTCAAGTTCTAGGCGGATTTTGATTCTTGTTCTTGTTCTTCCTTTATGGAGGATTCACCTATTGCATTTTGTTACCTGCAACTTTTGTTTTTAAACACATTTTAATCTGTGGAAGGTTGAAGAACAACTTGTTAATCTGCAATGCACCTACACATCTGTTTTCATTCTTAGTTGTACGACATATATAAATGTTTTTTTTTTTTTTTTGGATAACCGTGAGTATCCAGACCAACTTACGCACACTTCGACTAATCTCACGGGACAACCCACCCGACCCTACAACAAGTGGGCTGCCAACTTACCCGTAGGATATAAATTCTTGAAAAGTACTTAGATGCATCCTGGGTAGCACTGCATCCCATCAAATTTTTCATTCATAATTTGTCACATGAAATATTTTAATTCTTATTTTATGTGTATGATGAAATTGGTGAACATAAGTATTTCTCTAAATTCTTTAAAACAATTCTATTGTAGTTTAATGTTGTTTAATCCGTCCAAACATATCTCCATTGTTAGATAGTAATTTTTTATATCTAGGGTCTACATCACACTCGTATAGAAAAAGATACACCAATAGTTGTAATCAAGAAAAGACATGACTATCCATTCAAATATGTCTCTATGGTTTGATTATGATGTTTCATATTTATGTGGTTTAGATTACACTCGTACATAAAAGAATACATCACTTTACGTGTAATCACTTCGACTCAAATAGTTGTAACAAAGGACAAAATAGAAATTATGAAAAGTTGTAAGGGCGAAATGAGGACGTAAAGGTATATAATATAAATTATTTGCTACCTTACCATTTGGGGTTGTTTAAAAGTTTAAAACTAAGCTATGAACATCTATATTGGATCAGGGATCTTGCGGATCCTGCTGGAGTTTCAGTACAACCGGTGCCCTTGAAGGTGCTAACTTCCTTGCCACAGGGGAACTTGTTAGCTTAAGCGAACAGCAGCTGGTAGATTGTGATCATGAGGTTTGATTCCTTCTTTGTTTTTAATTAGCTTCGTTACACGTATAAATATATCGTGATAGCTATGTAATAGCTTTGTTTAAATTTAATATGTGATTAATTTCTTGTGGATGTCAGGATGCTTAATCAACTCTTGATGTTTCCTCTGGATTTTCTCTTAGATGCATTGGTTGTTGTGTTGGGTTATAGTTAATTTTCTCCTTCACTAGCTAGAAATGTTCTGACGATTGCTTTGTTTGATTATTATTCTTGTTTTGATGATGATAGCTCATGTTCTTTATTTATGAAATTTTATATGTAATGTGAAAATTAGAAGATTCGGACATGTTTGAGAGTGATTTTAAACTCTCATCAATTTCAAAATCACTCTTAAAATATACTTTAAATTGTATGTAAATTGCATTCAAAAGTGTAAAATCAAACATTAAGTTAGTTTTAAATGATTAAAGACATGTTTTGTATAAATTTTGAACCTGACAAAAGTGATATTACCATTTCAAAATCACCTCAAATATGCTATTGTTGAAGGAGAATCCTTTCCAAATGTCTGGTTTTCCTTTAATGGTACTATTCGTGGTTAGAATTAGGGCACGTGTGGAAGTGATTCTAAAACTTTTGTTAAGTTCAAAATCACTCTCAAACATAGTTTAAATCATTTCATATCAATTTTGATTGTATGCAAATCACATTCAAAAGTACAAAATCAAATATGAAATTGATTTTGTATGGATTTTAAACCTGAGAAAAGTGAAATTACCATTTCAAAATCACCTCAATCATGCCATTAGGCTATGATTGAAGGAGATTCCTTTCCATATGCCTGATTTTCCCTTTACTGATACAATTCAGAAACCTTTTAACTTCCATTTCTAAATTTTGCATATAATCTTCTATACTTTGCAGTGTGATCCAGCGGAAGCTGATTCCTGTGACTCTGGTTGTAATGGTGGTCTAATGAATAGTGCATTTGAATACACATTAAAAGCCGGAGGTCTGATGAGGGAGGAAGATTATCCCTATACTGGAACTGATCGTGGAACCTGCAAGTTTGACAAATCCAAGATTGCTGCATCAGTTGCCAATTTCAGCGTTGTTTCACTTGATGAAAATCAAATTGCTGCAAATCTGGTGAAAAATGGCCCACTTGCAAGTAAAATCCCATGCCTTTCATTATTCTGTTTAGGATACTTCCTAACAACTACACTCAAGAACAACACAAAAAACTCGAGAACACTTGAATAGAGAAGTAATAAAATAACCTAGTCTTTCGAGAGACTAGTCTCTCTCAGATTCCCAAAAAAAAATCCCACAAAATTCTTCACACTTTTGCTCCCAACTCACTCCCTCTATTTATAACCAAAAGTCTTAAAAAACTTGCTGTCTAATTACTAATATACCTCTTCTAATAACCATACTAATATTCTACTGATAATCCTATATATCTCTAACTAGGGCTCTCACATGTTGTTATGCAAATTCAATATGTTCTTAAAAACTTCCATTATGGGTCTAAATTCTGGGTTTTTTTTTTTTTTACCTCTATGCAGTTGCTATCAATGCAGTGTTCATGCAAACATATATAGGCGGAGTATCTTGTCCATTCATATGTTCAAAGCGATTGGATCATGGAGTTTTGTTGGTGGGTTATGGCTCAGCTGGCTATGCTCCCATCAGAATGAGAGACAAAGATTATTGGATCATAAAAAATTCTTGGGGCGAAAACTGGGGAGAAAATGGCTATTATAGGATCTGCAGAGGAAGGAATATTTGTGGAGTTGATTCCTTGGTCTCAACTGTTGCTGCAGTTCATACTGATGATGCCTCCATTGCAGCAGCAGCAGCAGGTCAGTAG

mRNA sequence

ATGGATCGGAATTTCTTCTTGTTCGCCGTGATCGCCGCCACCTTCTGCTCATCGGAATCTTTGGCCTCACAGCATTCCGTCGAACGAAATGGCGATCCGCTGATTCGTCAAGTCGTCGACGATGGCGATTTCAATCGTCTTCCTCTCGGAGCGGAGCACCATTTTTCGCTCTTCAAGCGAAAGTTCGGGAAATCGTATGCCACTGAGGAAGAGCACGATCTTAGATTCAAGATCTTCAAGGCTAATATGCGACGAGCGCAGCTCCATCAGTCATTTGATCCTTCCGCCATTCACGGCGTCACTCAGTTCGCCGATTTGACCCCTTTCGAGTTTCGGAAGGCATTTCTAGGGCTCCGAGGTCACCGTCTCAGGCTTCCTGTTGATACTAACGCAGCTCCGATTCTTCCTACGGAGAATCTTCCGATTGATTTTGATTGGAGAGAACGTGGTGCTGTAACTCCGGTGAAAAATCAGGGATCTTGCGGATCCTGCTGGAGTTTCAGTACAACCGGTGCCCTTGAAGGTGCTAACTTCCTTGCCACAGGGGAACTTGTTAGCTTAAGCGAACAGCAGCTGGTAGATTGTGATCATGAGTGTGATCCAGCGGAAGCTGATTCCTGTGACTCTGGTTGTAATGGTGGTCTAATGAATAGTGCATTTGAATACACATTAAAAGCCGGAGGTCTGATGAGGGAGGAAGATTATCCCTATACTGGAACTGATCGTGGAACCTGCAAGTTTGACAAATCCAAGATTGCTGCATCAGTTGCCAATTTCAGCGTTGTTTCACTTGATGAAAATCAAATTGCTGCAAATCTGGTGAAAAATGGCCCACTTGCAATTGCTATCAATGCAGTGTTCATGCAAACATATATAGGCGGAGTATCTTGTCCATTCATATGTTCAAAGCGATTGGATCATGGAGTTTTGTTGGTGGGTTATGGCTCAGCTGGCTATGCTCCCATCAGAATGAGAGACAAAGATTATTGGATCATAAAAAATTCTTGGGGCGAAAACTGGGGAGAAAATGGCTATTATAGGATCTGCAGAGGAAGGAATATTTGTGGAGTTGATTCCTTGGTCTCAACTGTTGCTGCAGTTCATACTGATGATGCCTCCATTGCAGCAGCAGCAGCAGGTCAGTAG

Coding sequence (CDS)

ATGGATCGGAATTTCTTCTTGTTCGCCGTGATCGCCGCCACCTTCTGCTCATCGGAATCTTTGGCCTCACAGCATTCCGTCGAACGAAATGGCGATCCGCTGATTCGTCAAGTCGTCGACGATGGCGATTTCAATCGTCTTCCTCTCGGAGCGGAGCACCATTTTTCGCTCTTCAAGCGAAAGTTCGGGAAATCGTATGCCACTGAGGAAGAGCACGATCTTAGATTCAAGATCTTCAAGGCTAATATGCGACGAGCGCAGCTCCATCAGTCATTTGATCCTTCCGCCATTCACGGCGTCACTCAGTTCGCCGATTTGACCCCTTTCGAGTTTCGGAAGGCATTTCTAGGGCTCCGAGGTCACCGTCTCAGGCTTCCTGTTGATACTAACGCAGCTCCGATTCTTCCTACGGAGAATCTTCCGATTGATTTTGATTGGAGAGAACGTGGTGCTGTAACTCCGGTGAAAAATCAGGGATCTTGCGGATCCTGCTGGAGTTTCAGTACAACCGGTGCCCTTGAAGGTGCTAACTTCCTTGCCACAGGGGAACTTGTTAGCTTAAGCGAACAGCAGCTGGTAGATTGTGATCATGAGTGTGATCCAGCGGAAGCTGATTCCTGTGACTCTGGTTGTAATGGTGGTCTAATGAATAGTGCATTTGAATACACATTAAAAGCCGGAGGTCTGATGAGGGAGGAAGATTATCCCTATACTGGAACTGATCGTGGAACCTGCAAGTTTGACAAATCCAAGATTGCTGCATCAGTTGCCAATTTCAGCGTTGTTTCACTTGATGAAAATCAAATTGCTGCAAATCTGGTGAAAAATGGCCCACTTGCAATTGCTATCAATGCAGTGTTCATGCAAACATATATAGGCGGAGTATCTTGTCCATTCATATGTTCAAAGCGATTGGATCATGGAGTTTTGTTGGTGGGTTATGGCTCAGCTGGCTATGCTCCCATCAGAATGAGAGACAAAGATTATTGGATCATAAAAAATTCTTGGGGCGAAAACTGGGGAGAAAATGGCTATTATAGGATCTGCAGAGGAAGGAATATTTGTGGAGTTGATTCCTTGGTCTCAACTGTTGCTGCAGTTCATACTGATGATGCCTCCATTGCAGCAGCAGCAGCAGGTCAGTAG

Protein sequence

MDRNFFLFAVIAATFCSSESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFSLFKRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGLRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYTGTDRGTCKFDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGVDSLVSTVAAVHTDDASIAAAAAGQ

Homology

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BLAST of HG10005743.1 vs. NCBI nr
Match: XP_038887474.1 (probable cysteine protease RD19B [Benincasa hispida])

HSP 1 Score: 721.5 bits (1861), Expect = 3.9e-204
Identity = 352/381 (92.39%), Postives = 364/381 (95.54%), Query Frame = 0


Query: 1   MDRNFFLFAVIA---ATFCSSESLASQHSVERNGDPLIRQVVDDGDF-NRLPLGAEHHFS 60
           MD +F LFAVIA   AT CSSESL S  S+ER+GDPLIRQVVDDG+F NRLPLGAEHHFS
Sbjct: 1   MDWSFSLFAVIAAATATLCSSESLTSPRSIERDGDPLIRQVVDDGNFNNRLPLGAEHHFS 60

Query: 61  LFKRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFL 120
           LFK++FGKSYATEEEHD RFKIF+ANMRRAQ HQSFDPSAIHG+TQF+DLTPFEFRKAFL
Sbjct: 61  LFKQRFGKSYATEEEHDRRFKIFEANMRRAQRHQSFDPSAIHGITQFSDLTPFEFRKAFL 120

Query: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGA 180
           GLRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGA 180

Query: 181 NFLATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYP 240
           NFLATGELVSLSEQQLVDCDHECDP EADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYP
Sbjct: 181 NFLATGELVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYP 240

Query: 241 YTGTDRGTCKFDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVS 300
           YTGTDRG C FDKSKIAASVANFSVVSLDE+QIAANLVKNGPLAIAINAVFMQTYIGGVS
Sbjct: 241 YTGTDRGNCNFDKSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS 300

Query: 301 CPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICG 360
           CPFICS+RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYY+ICRGRNICG
Sbjct: 301 CPFICSRRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYKICRGRNICG 360

Query: 361 VDSLVSTVAAVHTDDASIAAA 378
           VDSLVSTVAAVHT D SIAAA
Sbjct: 361 VDSLVSTVAAVHTHDTSIAAA 381

[Close]

BLAST of HG10005743.1 vs. NCBI nr
Match: XP_008448073.1 (PREDICTED: cysteine proteinase RD19a-like [Cucumis melo])

HSP 1 Score: 689.5 bits (1778), Expect = 1.6e-194
Identity = 337/380 (88.68%), Postives = 352/380 (92.63%), Query Frame = 0


Query: 1   MDRNFFLFAVI-AATFCSSESLASQHSVERNGDPLIRQVV-DDGDFNRLPLGAEHHFSLF 60
           MD NFFLFAVI AAT C SESL S HSV+ + DP IRQVV DDGDFNR  LGAEHHFSLF
Sbjct: 1   MDLNFFLFAVITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDGDFNRHALGAEHHFSLF 60

Query: 61  KRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGL 120
           KR+FGKSYATEEEHD RFKIFKANMRRA+ HQSFDPSAIHG+TQF+DLTPFEFRKAFLGL
Sbjct: 61  KRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLGL 120

Query: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANF 180
           RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVT VKNQGSCGSCWSFSTTGA+EGANF
Sbjct: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEGANF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYT 240
           LATG+LVSLSEQQLVDCDHECDP E D+CDSGCNGGLMNSAFEYTLKAGGLM+E+DYPYT
Sbjct: 181 LATGKLVSLSEQQLVDCDHECDPEEEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYPYT 240

Query: 241 GTDRGTCKFDKSKIAASVANFSVV-SLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300
           GTD  TC FDKSKIAAS+ANFSVV SLDE+QIAANLVKNGPLAIAINAVFMQTYIGGVSC
Sbjct: 241 GTDHSTCNFDKSKIAASIANFSVVNSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300

Query: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGV 360
           PFICS RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYY+ICRGRNICGV
Sbjct: 301 PFICSNRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYKICRGRNICGV 360

Query: 361 DSLVSTVAAVHTDDASIAAA 378
           DSLVSTVAAVH    S  A+
Sbjct: 361 DSLVSTVAAVHIHHTSSIAS 380

[Close]

BLAST of HG10005743.1 vs. NCBI nr
Match: XP_004148554.2 (cysteine protease RD19A [Cucumis sativus] >KGN43159.1 hypothetical protein Csa_020172 [Cucumis sativus])

HSP 1 Score: 688.3 bits (1775), Expect = 3.7e-194
Identity = 337/384 (87.76%), Postives = 354/384 (92.19%), Query Frame = 0


Query: 1   MDRNFFLFAVI---AATFCSSESLASQHSVERNGDPLIRQVVD-DGDFNRLPLGAEHHFS 60
           MDRNFFLFAVI    AT CSSE L SQHSVE +GDPLIRQVV+ DGDFN   LGAEHHFS
Sbjct: 1   MDRNFFLFAVITAVTATLCSSEPLVSQHSVEHDGDPLIRQVVENDGDFNHHALGAEHHFS 60

Query: 61  LFKRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFL 120
           LFKR+FGKSYATEEEHD RFKIFKANMRRA+ HQSFDPSAIHGVTQF+DLTPFEFRKAFL
Sbjct: 61  LFKRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGVTQFSDLTPFEFRKAFL 120

Query: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGA 180
           GLRGHRLRLPVDTNAAPILPTENLPIDFDWR+ G VT VKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRQHGGVTRVKNQGSCGSCWSFSTTGALEGA 180

Query: 181 NFLATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYP 240
           NFLATGELVSLSEQQLVDCDHECDP E D+CDSGCNGGLMNSAFEYTLKAGGLM+E+DYP
Sbjct: 181 NFLATGELVSLSEQQLVDCDHECDPEEEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYP 240

Query: 241 YTGTDRGTCKFDKSKIAASVANFSVV-SLDENQIAANLVKNGPLAIAINAVFMQTYIGGV 300
           Y G DR TC FDKSKIAAS+A+FSVV S+DE+QIAANLVKNGPLAIAINAVFMQTYIGGV
Sbjct: 241 YAGIDRNTCNFDKSKIAASIASFSVVNSIDEDQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 301 SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNIC 360
           SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGE+WGENGYY+ICRGRNIC
Sbjct: 301 SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGESWGENGYYKICRGRNIC 360

Query: 361 GVDSLVSTVAAVHTDDASIAAAAA 380
           GVDSLVSTVAAVH    S + A+A
Sbjct: 361 GVDSLVSTVAAVHIHHHSSSIASA 384

[Close]

BLAST of HG10005743.1 vs. NCBI nr
Match: KAA0033790.1 (cysteine proteinase RD19a-like [Cucumis melo var. makuwa])

HSP 1 Score: 673.7 bits (1737), Expect = 9.3e-190
Identity = 333/380 (87.63%), Postives = 348/380 (91.58%), Query Frame = 0


Query: 1   MDRNFFLFAVI-AATFCSSESLASQHSVERNGDPLIRQVV-DDGDFNRLPLGAEHHFSLF 60
           MD NFFLFAVI AAT C SESL S HSV+ + DP IRQVV DD DFNR  LGAEHHFSLF
Sbjct: 1   MDLNFFLFAVITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDSDFNRHALGAEHHFSLF 60

Query: 61  KRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGL 120
           KR+FGKSYATEEEHD RFKIFKANMRRA+ HQSFDPSAIHG+TQF+DLTPFEFRKAFLGL
Sbjct: 61  KRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLGL 120

Query: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANF 180
           RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVT VKNQGSCGSCWSFSTTGA+EGANF
Sbjct: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEGANF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYT 240
           LATG+LVSLSEQQLVDCDHE    E D+CDSGCNGGLMNSAFEYTLKAGGLM+E+DYPYT
Sbjct: 181 LATGKLVSLSEQQLVDCDHE----EEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYPYT 240

Query: 241 GTDRGTCKFDKSKIAASVANFSVV-SLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300
           GTD  TC FDKSKIAAS+ANFSVV SLDE+QIAANLVKNGPLAIAINAVFMQTYIGGVSC
Sbjct: 241 GTDHSTCNFDKSKIAASIANFSVVNSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300

Query: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGV 360
           PFICS RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYY+ICRGRNICGV
Sbjct: 301 PFICSNRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYKICRGRNICGV 360

Query: 361 DSLVSTVAAVHTDDASIAAA 378
           DSLVSTVAAVH    S  A+
Sbjct: 361 DSLVSTVAAVHIHHTSSIAS 376

[Close]

BLAST of HG10005743.1 vs. NCBI nr
Match: XP_022970957.1 (probable cysteine protease RD19B [Cucurbita maxima])

HSP 1 Score: 657.1 bits (1694), Expect = 9.0e-185
Identity = 322/377 (85.41%), Postives = 341/377 (90.45%), Query Frame = 0


Query: 1   MDRNFFLFAVI--AATFCSSESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFSLF 60
           MDR+F LF V+  AA  CSSESLAS +SV     PLIRQV+DDG+ N LPL AEHHF LF
Sbjct: 1   MDRSFLLFPVVIAAAVLCSSESLASTYSV----SPLIRQVIDDGESNNLPLQAEHHFLLF 60

Query: 61  KRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGL 120
           KRKFGKSYATEEEH+ RF+IF+ANMRRA  HQSFDPSAIHGVTQF+DLT  EF+K FLGL
Sbjct: 61  KRKFGKSYATEEEHNRRFRIFRANMRRALRHQSFDPSAIHGVTQFSDLTVSEFQKTFLGL 120

Query: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANF 180
           RGHRL+LP+  N APILPTENLP DFDWRERGAVTPVKNQG+CGSCWSFSTTGALEGANF
Sbjct: 121 RGHRLKLPLHANQAPILPTENLPGDFDWRERGAVTPVKNQGTCGSCWSFSTTGALEGANF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYT 240
           LATGELVSLSEQQLVDCDHECD  EA SCDSGCNGGLMNSA EYTLK GGLMRE+DYPYT
Sbjct: 181 LATGELVSLSEQQLVDCDHECDSEEAGSCDSGCNGGLMNSALEYTLKVGGLMREQDYPYT 240

Query: 241 GTDRGTCKFDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300
           GTDR TCKFD+SKIAASVANFSVVSLDE+QIAANLVKNGPLAIAINAVFMQTYIGGVSCP
Sbjct: 241 GTDRETCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300

Query: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGVD 360
           FICSKRLDHGVLLVGYGSAGYAPIRMRD+DYWIIKNSWG NWGENGYYRIC+GRNICGVD
Sbjct: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDRDYWIIKNSWGPNWGENGYYRICKGRNICGVD 360

Query: 361 SLVSTVAAVHTDDASIA 376
           SLVSTVAAVHT  A+ A
Sbjct: 361 SLVSTVAAVHTPIAAQA 373

[Close]

BLAST of HG10005743.1 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 9.1e-156
Identity = 269/366 (73.50%), Postives = 299/366 (81.69%), Query Frame = 0


Query: 1   MDRNFFLFAVIAATFCSSESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFSLFKR 60
           MDR    F+V   +F       S   V    D +IRQVV  G      L +E HFSLFKR
Sbjct: 1   MDRLKLYFSVFVLSFFIVS--VSSSDVNDGDDLVIRQVV--GGAEPQVLTSEDHFSLFKR 60

Query: 61  KFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGLRG 120
           KFGK YA+ EEHD RF +FKAN+RRA+ HQ  DPSA HGVTQF+DLT  EFRK  LG+R 
Sbjct: 61  KFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLGVRS 120

Query: 121 HRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANFLA 180
              +LP D N APILPTENLP DFDWR+ GAVTPVKNQGSCGSCWSFS TGALEGANFLA
Sbjct: 121 -GFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGANFLA 180

Query: 181 TGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYTGT 240
           TG+LVSLSEQQLVDCDHECDP EADSCDSGCNGGLMNSAFEYTLK GGLM+EEDYPYTG 
Sbjct: 181 TGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPYTGK 240

Query: 241 DRGTCKFDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFI 300
           D  TCK DKSKI ASV+NFSV+S+DE QIAANLVKNGPLA+AINA +MQTYIGGVSCP+I
Sbjct: 241 DGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYI 300

Query: 301 CSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGVDSL 360
           C++RL+HGVLLVGYG+AGYAP R ++K YWIIKNSWGE WGENG+Y+IC+GRNICGVDS+
Sbjct: 301 CTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDSM 360

Query: 361 VSTVAA 367
           VSTVAA
Sbjct: 361 VSTVAA 361

[Close]

BLAST of HG10005743.1 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 5.2e-151
Identity = 259/372 (69.62%), Postives = 308/372 (82.80%), Query Frame = 0


Query: 1   MDRNFFLFAVIAATFCSS--ESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFSLF 60
           MDR  F F + A     S   ++ S    +   +P IRQVV + +  +L L AEHHF+LF
Sbjct: 1   MDRVVFFFLIAATLLAGSLGSTVISGEVTDGFVNP-IRQVVPEENDEQL-LNAEHHFTLF 60

Query: 61  KRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGL 120
           K K+ K+YAT+ EHD RF++FKAN+RRA+ +Q  DPSA+HGVTQF+DLTP EFR+ FLGL
Sbjct: 61  KSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLGL 120

Query: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANF 180
           +    RLP DT  APILPT +LP +FDWRE+GAVTPVKNQG CGSCWSFS  GALEGA+F
Sbjct: 121 KRRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAHF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYT 240
           LAT ELVSLSEQQLVDCDHECDPA+A+SCDSGC+GGLMN+AFEY LKAGGLM+EEDYPYT
Sbjct: 181 LATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPYT 240

Query: 241 GTDRGTCKFDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300
           G D   CKFDKSKI ASV+NFSVVS DE+QIAANLV++GPLAIAINA++MQTYIGGVSCP
Sbjct: 241 GRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSCP 300

Query: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRG-RNICGV 360
           ++CSK  DHGVLLVG+GS+GYAPIR+++K YWIIKNSWG  WGE+GYY+ICRG  N+CG+
Sbjct: 301 YVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCGM 360

Query: 361 DSLVSTVAAVHT 370
           D++VSTVAAVHT
Sbjct: 361 DTMVSTVAAVHT 370

[Close]

BLAST of HG10005743.1 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 533.9 bits (1374), Expect = 1.5e-150
Identity = 255/360 (70.83%), Postives = 299/360 (83.06%), Query Frame = 0


Query: 7   LFAVIAATFCSSESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFSLFKRKFGKSY 66
           +F  ++ + C  E            D LIRQVVD+ +     L +E HF+LFK+KFGK Y
Sbjct: 14  IFVFVSVSVCGDE------------DVLIRQVVDETE--PKVLSSEDHFTLFKKKFGKVY 73

Query: 67  ATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGLRGHRLRLP 126
            + EEH  RF +FKAN+ RA  HQ  DPSA HGVTQF+DLT  EFR+  LG++G   +LP
Sbjct: 74  GSIEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVKG-GFKLP 133

Query: 127 VDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANFLATGELVS 186
            D N APILPT+NLP +FDWR+RGAVTPVKNQGSCGSCWSFSTTGALEGA+FLATG+LVS
Sbjct: 134 KDANQAPILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKLVS 193

Query: 187 LSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYTGTDRGTCK 246
           LSEQQLVDCDHECDP E  SCDSGCNGGLMNSAFEYTLK GGLMRE+DYPYTGTD G+CK
Sbjct: 194 LSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGSCK 253

Query: 247 FDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFICSKRLD 306
            D+SKI ASV+NFSVVS++E+QIAANL+KNGPLA+AINA +MQTYIGGVSCP+ICS+RL+
Sbjct: 254 LDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRRLN 313

Query: 307 HGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGVDSLVSTVAA 366
           HGVLLVGYGSAG++  R+++K YWIIKNSWGE+WGENG+Y+IC+GRNICGVDSLVSTVAA
Sbjct: 314 HGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAA 358

[Close]

BLAST of HG10005743.1 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.0e-146
Identity = 252/375 (67.20%), Postives = 307/375 (81.87%), Query Frame = 0


Query: 1   MDRNF----FLFAVIAATFCSSESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFS 60
           MDR F    FLFA +A       +         N D +IRQVVD+ + +   L AEHHF+
Sbjct: 1   MDRRFLFALFLFAAVATAVTDDTN---------NDDFIIRQVVDNEEDHL--LNAEHHFT 60

Query: 61  LFKRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFL 120
            FK KF KSYAT+EEHD RF +FK+N+ +A+LHQ+ DP+A HG+T+F+DLT  EFR+ FL
Sbjct: 61  SFKSKFSKSYATKEEHDYRFGVFKSNLIKAKLHQNRDPTAEHGITKFSDLTASEFRRQFL 120

Query: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGA 180
           GL+  RLRLP     APILPT NLP DFDWRE+GAVTPVK+QGSCGSCW+FSTTGALEGA
Sbjct: 121 GLK-KRLRLPAHAQKAPILPTTNLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGA 180

Query: 181 NFLATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYP 240
           ++LATG+LVSLSEQQLVDCDH CDP +A SCDSGCNGGLMN+AFEY L++GG+++E+DY 
Sbjct: 181 HYLATGKLVSLSEQQLVDCDHVCDPEQAGSCDSGCNGGLMNNAFEYLLESGGVVQEKDYA 240

Query: 241 YTGTDRGTCKFDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVS 300
           YTG D G+CKFDKSK+ ASV+NFSVV+LDE+QIAANLVKNGPLA+AINA +MQTY+ GVS
Sbjct: 241 YTGRD-GSCKFDKSKVVASVSNFSVVTLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVS 300

Query: 301 CPFICSK-RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNIC 360
           CP++C+K RLDHGVLLVG+G   YAPIR+++K YWIIKNSWG+NWGE GYY+ICRGRN+C
Sbjct: 301 CPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVC 360

Query: 361 GVDSLVSTVAAVHTD 371
           GVDS+VSTVAA  ++
Sbjct: 361 GVDSMVSTVAAAQSN 362

[Close]

BLAST of HG10005743.1 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 1.8e-140
Identity = 240/344 (69.77%), Postives = 277/344 (80.52%), Query Frame = 0


Query: 32  DPLIRQVVDDGDFNRLPLGAEHHFSLFKRKFGKSYATEEEHDLRFKIFKANMRRAQLHQS 91
           DPLIRQVV  GD N L L AE HF  F ++FGKSY   +EH  R  +FK N+RRA+ HQ 
Sbjct: 25  DPLIRQVVPGGDDNDLELNAESHFLSFVQRFGKSYKDADEHAYRLSVFKDNLRRARRHQL 84

Query: 92  FDPSAIHGVTQFADLTPFEFRKAFLGLRGHR----LRLPVDTNAAPILPTENLPIDFDWR 151
            DPSA HGVT+F+DLTP EFR+ +LGLR  R      L    + AP+LPT+ LP DFDWR
Sbjct: 85  LDPSAEHGVTKFSDLTPAEFRRTYLGLRKSRRALLRELGESAHEAPVLPTDGLPDDFDWR 144

Query: 152 ERGAVTPVKNQGSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHECDPAEADSC 211
           + GAV PVKNQGSCGSCWSFS +GALEGA++LATG+L  LSEQQ VDCDHECD +E DSC
Sbjct: 145 DHGAVGPVKNQGSCGSCWSFSASGALEGAHYLATGKLEVLSEQQFVDCDHECDSSEPDSC 204

Query: 212 DSGCNGGLMNSAFEYTLKAGGLMREEDYPYTGTDRGTCKFDKSKIAASVANFSVVSLDEN 271
           DSGCNGGLM +AF Y  KAGGL  E+DYPYTG+D G CKFDKSKI ASV NFSVVS+DE 
Sbjct: 205 DSGCNGGLMTTAFSYLQKAGGLESEKDYPYTGSD-GKCKFDKSKIVASVQNFSVVSVDEA 264

Query: 272 QIAANLVKNGPLAIAINAVFMQTYIGGVSCPFICSKRLDHGVLLVGYGSAGYAPIRMRDK 331
           QI+ANL+K+GPLAI INA +MQTYIGGVSCP+IC + LDHGVLLVGYG++G+APIR++DK
Sbjct: 265 QISANLIKHGPLAIGINAAYMQTYIGGVSCPYICGRHLDHGVLLVGYGASGFAPIRLKDK 324

Query: 332 DYWIIKNSWGENWGENGYYRICRG---RNICGVDSLVSTVAAVH 369
            YWIIKNSWGENWGENGYY+ICRG   RN CGVDS+VSTV+AVH
Sbjct: 325 PYWIIKNSWGENWGENGYYKICRGSNVRNKCGVDSMVSTVSAVH 367

[Close]

BLAST of HG10005743.1 vs. ExPASy TrEMBL
Match: A0A1S3BIU9 (cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103490359 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 7.9e-195
Identity = 337/380 (88.68%), Postives = 352/380 (92.63%), Query Frame = 0


Query: 1   MDRNFFLFAVI-AATFCSSESLASQHSVERNGDPLIRQVV-DDGDFNRLPLGAEHHFSLF 60
           MD NFFLFAVI AAT C SESL S HSV+ + DP IRQVV DDGDFNR  LGAEHHFSLF
Sbjct: 1   MDLNFFLFAVITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDGDFNRHALGAEHHFSLF 60

Query: 61  KRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGL 120
           KR+FGKSYATEEEHD RFKIFKANMRRA+ HQSFDPSAIHG+TQF+DLTPFEFRKAFLGL
Sbjct: 61  KRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLGL 120

Query: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANF 180
           RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVT VKNQGSCGSCWSFSTTGA+EGANF
Sbjct: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEGANF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYT 240
           LATG+LVSLSEQQLVDCDHECDP E D+CDSGCNGGLMNSAFEYTLKAGGLM+E+DYPYT
Sbjct: 181 LATGKLVSLSEQQLVDCDHECDPEEEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYPYT 240

Query: 241 GTDRGTCKFDKSKIAASVANFSVV-SLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300
           GTD  TC FDKSKIAAS+ANFSVV SLDE+QIAANLVKNGPLAIAINAVFMQTYIGGVSC
Sbjct: 241 GTDHSTCNFDKSKIAASIANFSVVNSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300

Query: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGV 360
           PFICS RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYY+ICRGRNICGV
Sbjct: 301 PFICSNRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYKICRGRNICGV 360

Query: 361 DSLVSTVAAVHTDDASIAAA 378
           DSLVSTVAAVH    S  A+
Sbjct: 361 DSLVSTVAAVHIHHTSSIAS 380

[Close]

BLAST of HG10005743.1 vs. ExPASy TrEMBL
Match: A0A0A0K2B5 (Papain-like cysteine proteinase isoform I OS=Cucumis sativus OX=3659 GN=Csa_7G004120 PE=3 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 1.8e-194
Identity = 337/384 (87.76%), Postives = 354/384 (92.19%), Query Frame = 0


Query: 1   MDRNFFLFAVI---AATFCSSESLASQHSVERNGDPLIRQVVD-DGDFNRLPLGAEHHFS 60
           MDRNFFLFAVI    AT CSSE L SQHSVE +GDPLIRQVV+ DGDFN   LGAEHHFS
Sbjct: 1   MDRNFFLFAVITAVTATLCSSEPLVSQHSVEHDGDPLIRQVVENDGDFNHHALGAEHHFS 60

Query: 61  LFKRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFL 120
           LFKR+FGKSYATEEEHD RFKIFKANMRRA+ HQSFDPSAIHGVTQF+DLTPFEFRKAFL
Sbjct: 61  LFKRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGVTQFSDLTPFEFRKAFL 120

Query: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGA 180
           GLRGHRLRLPVDTNAAPILPTENLPIDFDWR+ G VT VKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRQHGGVTRVKNQGSCGSCWSFSTTGALEGA 180

Query: 181 NFLATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYP 240
           NFLATGELVSLSEQQLVDCDHECDP E D+CDSGCNGGLMNSAFEYTLKAGGLM+E+DYP
Sbjct: 181 NFLATGELVSLSEQQLVDCDHECDPEEEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYP 240

Query: 241 YTGTDRGTCKFDKSKIAASVANFSVV-SLDENQIAANLVKNGPLAIAINAVFMQTYIGGV 300
           Y G DR TC FDKSKIAAS+A+FSVV S+DE+QIAANLVKNGPLAIAINAVFMQTYIGGV
Sbjct: 241 YAGIDRNTCNFDKSKIAASIASFSVVNSIDEDQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 301 SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNIC 360
           SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGE+WGENGYY+ICRGRNIC
Sbjct: 301 SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGESWGENGYYKICRGRNIC 360

Query: 361 GVDSLVSTVAAVHTDDASIAAAAA 380
           GVDSLVSTVAAVH    S + A+A
Sbjct: 361 GVDSLVSTVAAVHIHHHSSSIASA 384

[Close]

BLAST of HG10005743.1 vs. ExPASy TrEMBL
Match: A0A5A7SS65 (Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold142G00480 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.5e-190
Identity = 333/380 (87.63%), Postives = 348/380 (91.58%), Query Frame = 0


Query: 1   MDRNFFLFAVI-AATFCSSESLASQHSVERNGDPLIRQVV-DDGDFNRLPLGAEHHFSLF 60
           MD NFFLFAVI AAT C SESL S HSV+ + DP IRQVV DD DFNR  LGAEHHFSLF
Sbjct: 1   MDLNFFLFAVITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDSDFNRHALGAEHHFSLF 60

Query: 61  KRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGL 120
           KR+FGKSYATEEEHD RFKIFKANMRRA+ HQSFDPSAIHG+TQF+DLTPFEFRKAFLGL
Sbjct: 61  KRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLGL 120

Query: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANF 180
           RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVT VKNQGSCGSCWSFSTTGA+EGANF
Sbjct: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEGANF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYT 240
           LATG+LVSLSEQQLVDCDHE    E D+CDSGCNGGLMNSAFEYTLKAGGLM+E+DYPYT
Sbjct: 181 LATGKLVSLSEQQLVDCDHE----EEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYPYT 240

Query: 241 GTDRGTCKFDKSKIAASVANFSVV-SLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300
           GTD  TC FDKSKIAAS+ANFSVV SLDE+QIAANLVKNGPLAIAINAVFMQTYIGGVSC
Sbjct: 241 GTDHSTCNFDKSKIAASIANFSVVNSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300

Query: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGV 360
           PFICS RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYY+ICRGRNICGV
Sbjct: 301 PFICSNRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYKICRGRNICGV 360

Query: 361 DSLVSTVAAVHTDDASIAAA 378
           DSLVSTVAAVH    S  A+
Sbjct: 361 DSLVSTVAAVHIHHTSSIAS 376

[Close]

BLAST of HG10005743.1 vs. ExPASy TrEMBL
Match: A0A6J1I0L8 (probable cysteine protease RD19B OS=Cucurbita maxima OX=3661 GN=LOC111469766 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 4.4e-185
Identity = 322/377 (85.41%), Postives = 341/377 (90.45%), Query Frame = 0


Query: 1   MDRNFFLFAVI--AATFCSSESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFSLF 60
           MDR+F LF V+  AA  CSSESLAS +SV     PLIRQV+DDG+ N LPL AEHHF LF
Sbjct: 1   MDRSFLLFPVVIAAAVLCSSESLASTYSV----SPLIRQVIDDGESNNLPLQAEHHFLLF 60

Query: 61  KRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGL 120
           KRKFGKSYATEEEH+ RF+IF+ANMRRA  HQSFDPSAIHGVTQF+DLT  EF+K FLGL
Sbjct: 61  KRKFGKSYATEEEHNRRFRIFRANMRRALRHQSFDPSAIHGVTQFSDLTVSEFQKTFLGL 120

Query: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANF 180
           RGHRL+LP+  N APILPTENLP DFDWRERGAVTPVKNQG+CGSCWSFSTTGALEGANF
Sbjct: 121 RGHRLKLPLHANQAPILPTENLPGDFDWRERGAVTPVKNQGTCGSCWSFSTTGALEGANF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYT 240
           LATGELVSLSEQQLVDCDHECD  EA SCDSGCNGGLMNSA EYTLK GGLMRE+DYPYT
Sbjct: 181 LATGELVSLSEQQLVDCDHECDSEEAGSCDSGCNGGLMNSALEYTLKVGGLMREQDYPYT 240

Query: 241 GTDRGTCKFDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300
           GTDR TCKFD+SKIAASVANFSVVSLDE+QIAANLVKNGPLAIAINAVFMQTYIGGVSCP
Sbjct: 241 GTDRETCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300

Query: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGVD 360
           FICSKRLDHGVLLVGYGSAGYAPIRMRD+DYWIIKNSWG NWGENGYYRIC+GRNICGVD
Sbjct: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDRDYWIIKNSWGPNWGENGYYRICKGRNICGVD 360

Query: 361 SLVSTVAAVHTDDASIA 376
           SLVSTVAAVHT  A+ A
Sbjct: 361 SLVSTVAAVHTPIAAQA 373

[Close]

BLAST of HG10005743.1 vs. ExPASy TrEMBL
Match: A0A6J1F4R7 (probable cysteine protease RD19B OS=Cucurbita moschata OX=3662 GN=LOC111440857 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 7.4e-185
Identity = 320/372 (86.02%), Postives = 339/372 (91.13%), Query Frame = 0


Query: 1   MDRNFFLFAVI---AATFCSSESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFSL 60
           MDR+FFLF+V+   AA  CSSESLAS +SV     PLIRQV+DDG+ + LPL AEHHF L
Sbjct: 1   MDRSFFLFSVVIAAAAALCSSESLASTYSV----SPLIRQVIDDGESSNLPLQAEHHFLL 60

Query: 61  FKRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLG 120
           FKRKFGKSYATEEEH+ RF+IFKANMRRA  HQSFDPSAIHGVTQF+DLT  EF+K FLG
Sbjct: 61  FKRKFGKSYATEEEHNRRFRIFKANMRRALRHQSFDPSAIHGVTQFSDLTVSEFQKTFLG 120

Query: 121 LRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGAN 180
           LRGHRL+LP+D N APILPTENLP  FDWRERGAVTPVKNQG+CGSCWSFSTTGALEGAN
Sbjct: 121 LRGHRLKLPLDANQAPILPTENLPGGFDWRERGAVTPVKNQGTCGSCWSFSTTGALEGAN 180

Query: 181 FLATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPY 240
           FLATGELVSLSEQQLVDCDHECD  EA SCDSGCNGGLMNSA EYTLK GGLMRE+DYPY
Sbjct: 181 FLATGELVSLSEQQLVDCDHECDSEEAGSCDSGCNGGLMNSALEYTLKVGGLMREQDYPY 240

Query: 241 TGTDRGTCKFDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300
           TGTDR TCKFD+SKIAASVANFSVVSLDE+QIAANLVKNGPLAIAINAVFMQTYIGGVSC
Sbjct: 241 TGTDRETCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300

Query: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGV 360
           PFICSKRLDHGVLLVGYGSAGYAPIRMRD+DYWIIKNSWG NWGENGYYRIC+GRNICGV
Sbjct: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDRDYWIIKNSWGPNWGENGYYRICKGRNICGV 360

Query: 361 DSLVSTVAAVHT 370
           DSLVSTVAAV T
Sbjct: 361 DSLVSTVAAVQT 368

[Close]

BLAST of HG10005743.1 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 551.2 bits (1419), Expect = 6.5e-157
Identity = 269/366 (73.50%), Postives = 299/366 (81.69%), Query Frame = 0


Query: 1   MDRNFFLFAVIAATFCSSESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFSLFKR 60
           MDR    F+V   +F       S   V    D +IRQVV  G      L +E HFSLFKR
Sbjct: 1   MDRLKLYFSVFVLSFFIVS--VSSSDVNDGDDLVIRQVV--GGAEPQVLTSEDHFSLFKR 60

Query: 61  KFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGLRG 120
           KFGK YA+ EEHD RF +FKAN+RRA+ HQ  DPSA HGVTQF+DLT  EFRK  LG+R 
Sbjct: 61  KFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLGVRS 120

Query: 121 HRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANFLA 180
              +LP D N APILPTENLP DFDWR+ GAVTPVKNQGSCGSCWSFS TGALEGANFLA
Sbjct: 121 -GFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGANFLA 180

Query: 181 TGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYTGT 240
           TG+LVSLSEQQLVDCDHECDP EADSCDSGCNGGLMNSAFEYTLK GGLM+EEDYPYTG 
Sbjct: 181 TGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPYTGK 240

Query: 241 DRGTCKFDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFI 300
           D  TCK DKSKI ASV+NFSV+S+DE QIAANLVKNGPLA+AINA +MQTYIGGVSCP+I
Sbjct: 241 DGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYI 300

Query: 301 CSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGVDSL 360
           C++RL+HGVLLVGYG+AGYAP R ++K YWIIKNSWGE WGENG+Y+IC+GRNICGVDS+
Sbjct: 301 CTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDSM 360

Query: 361 VSTVAA 367
           VSTVAA
Sbjct: 361 VSTVAA 361

[Close]

BLAST of HG10005743.1 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 535.4 bits (1378), Expect = 3.7e-152
Identity = 259/372 (69.62%), Postives = 308/372 (82.80%), Query Frame = 0


Query: 1   MDRNFFLFAVIAATFCSS--ESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFSLF 60
           MDR  F F + A     S   ++ S    +   +P IRQVV + +  +L L AEHHF+LF
Sbjct: 1   MDRVVFFFLIAATLLAGSLGSTVISGEVTDGFVNP-IRQVVPEENDEQL-LNAEHHFTLF 60

Query: 61  KRKFGKSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGL 120
           K K+ K+YAT+ EHD RF++FKAN+RRA+ +Q  DPSA+HGVTQF+DLTP EFR+ FLGL
Sbjct: 61  KSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLGL 120

Query: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANF 180
           +    RLP DT  APILPT +LP +FDWRE+GAVTPVKNQG CGSCWSFS  GALEGA+F
Sbjct: 121 KRRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAHF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYT 240
           LAT ELVSLSEQQLVDCDHECDPA+A+SCDSGC+GGLMN+AFEY LKAGGLM+EEDYPYT
Sbjct: 181 LATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPYT 240

Query: 241 GTDRGTCKFDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300
           G D   CKFDKSKI ASV+NFSVVS DE+QIAANLV++GPLAIAINA++MQTYIGGVSCP
Sbjct: 241 GRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSCP 300

Query: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRG-RNICGV 360
           ++CSK  DHGVLLVG+GS+GYAPIR+++K YWIIKNSWG  WGE+GYY+ICRG  N+CG+
Sbjct: 301 YVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCGM 360

Query: 361 DSLVSTVAAVHT 370
           D++VSTVAAVHT
Sbjct: 361 DTMVSTVAAVHT 370

[Close]

BLAST of HG10005743.1 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 533.9 bits (1374), Expect = 1.1e-151
Identity = 255/360 (70.83%), Postives = 299/360 (83.06%), Query Frame = 0


Query: 7   LFAVIAATFCSSESLASQHSVERNGDPLIRQVVDDGDFNRLPLGAEHHFSLFKRKFGKSY 66
           +F  ++ + C  E            D LIRQVVD+ +     L +E HF+LFK+KFGK Y
Sbjct: 14  IFVFVSVSVCGDE------------DVLIRQVVDETE--PKVLSSEDHFTLFKKKFGKVY 73

Query: 67  ATEEEHDLRFKIFKANMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGLRGHRLRLP 126
            + EEH  RF +FKAN+ RA  HQ  DPSA HGVTQF+DLT  EFR+  LG++G   +LP
Sbjct: 74  GSIEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVKG-GFKLP 133

Query: 127 VDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANFLATGELVS 186
            D N APILPT+NLP +FDWR+RGAVTPVKNQGSCGSCWSFSTTGALEGA+FLATG+LVS
Sbjct: 134 KDANQAPILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKLVS 193

Query: 187 LSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYTGTDRGTCK 246
           LSEQQLVDCDHECDP E  SCDSGCNGGLMNSAFEYTLK GGLMRE+DYPYTGTD G+CK
Sbjct: 194 LSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGSCK 253

Query: 247 FDKSKIAASVANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFICSKRLD 306
            D+SKI ASV+NFSVVS++E+QIAANL+KNGPLA+AINA +MQTYIGGVSCP+ICS+RL+
Sbjct: 254 LDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRRLN 313

Query: 307 HGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGVDSLVSTVAA 366
           HGVLLVGYGSAG++  R+++K YWIIKNSWGE+WGENG+Y+IC+GRNICGVDSLVSTVAA
Sbjct: 314 HGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAA 358

[Close]

BLAST of HG10005743.1 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 413.3 bits (1061), Expect = 2.1e-115
Identity = 202/351 (57.55%), Postives = 254/351 (72.36%), Query Frame = 0


Query: 25  HSVERNGDPLIRQVVDDGDF---NRLPLGAEHHFSLFKRKFGKSYATEEEHDLRFKIFKA 84
           H V    D  IRQV  D      N L    E  F LF   +GK+Y+T EE+  R  IF  
Sbjct: 18  HVVASVEDLTIRQVTADNRRIRPNLLGTHTESKFRLFMSDYGKNYSTREEYIHRLGIFAK 77

Query: 85  NMRRAQLHQSFDPSAIHGVTQFADLTPFEFRKAFLGL------RGHRLRLPVDTNAAPIL 144
           N+ +A  HQ  DPSA+HGVTQF+DLT  EF++ + G+      RG  +        AP++
Sbjct: 78  NVLKAAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTGVADVGGSRGGTVGA-----EAPMV 137

Query: 145 PTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDC 204
             + LP DFDWRE+G VT VKNQG+CGSCW+FSTTGA EGA+F++TG+L+SLSEQQLVDC
Sbjct: 138 EVDGLPEDFDWREKGGVTEVKNQGACGSCWAFSTTGAAEGAHFVSTGKLLSLSEQQLVDC 197

Query: 205 DHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYTGTDRGTCKFDKSKIAAS 264
           D  CDP +  +CD+GC GGLM +A+EY ++AGGL  E  YPYTG  RG CKFD  K+A  
Sbjct: 198 DQACDPKDKKACDNGCGGGLMTNAYEYLMEAGGLEEERSYPYTG-KRGHCKFDPEKVAVR 257

Query: 265 VANFSVVSLDENQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFICSKR-LDHGVLLVGY 324
           V NF+ + LDENQIAANLV++GPLA+ +NAVFMQTYIGGVSCP ICSKR ++HGVLLVGY
Sbjct: 258 VLNFTTIPLDENQIAANLVRHGPLAVGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGY 317

Query: 325 GSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNICGVDSLVSTVA 366
           GS G++ +R+ +K YWIIKNSWG+ WGENGYY++CRG +ICG++S+VS VA
Sbjct: 318 GSKGFSILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSMVSAVA 362

[Close]

BLAST of HG10005743.1 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 231.9 bits (590), Expect = 8.7e-61
Identity = 131/309 (42.39%), Postives = 177/309 (57.28%), Query Frame = 0


Query: 64  KSYATEEEHDLRFKIFKANMRRAQLHQSFDPSAIH-GVTQFADLTPFEFRKAFLGLRGHR 123
           K+Y    E + RF+IFK N++  + H S        G+T+FADLT  EFR  +L  +  R
Sbjct: 52  KNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKMER 111

Query: 124 LRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGANFLATG 183
            R+PV          ++LP   DWR +GAV PVK+QGSCGSCW+FS  GA+EG N + TG
Sbjct: 112 TRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTG 171

Query: 184 ELVSLSEQQLVDCDHECDPAEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYPYTGTDR 243
           EL+SLSEQ+LVDCD         S + GC GGLM+ AF++ ++ GG+  EEDYPY  TD 
Sbjct: 172 ELISLSEQELVDCD--------TSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDV 231

Query: 244 GTCKFDKSKI-AASVANFSVVSLDENQIAANLVKNGPLAIAINA--VFMQTYIGGVSCPF 303
             C  DK      ++  +  V  ++ +     + N P+++AI A     Q Y  GV    
Sbjct: 232 NVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTG- 291

Query: 304 ICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYRICRGRNI----- 363
            C   LDHGV+ VGYGS G        +DYWI++NSWG NWGE+GY+++   RNI     
Sbjct: 292 TCGTSLDHGVVAVGYGSEG-------GQDYWIVRNSWGSNWGESGYFKL--ERNIKESSG 342

The following BLAST results are available for this feature:

BLAST of HG10005743.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887474.1	3.9e-204	92.39	probable cysteine protease RD19B [Benincasa hispida]	[more]
XP_008448073.1	1.6e-194	88.68	PREDICTED: cysteine proteinase RD19a-like [Cucumis melo]	[more]
XP_004148554.2	3.7e-194	87.76	cysteine protease RD19A [Cucumis sativus] >KGN43159.1 hypothetical protein Csa_0...	[more]
KAA0033790.1	9.3e-190	87.63	cysteine proteinase RD19a-like [Cucumis melo var. makuwa]	[more]
XP_022970957.1	9.0e-185	85.41	probable cysteine protease RD19B [Cucurbita maxima]	[more]

BLAST of HG10005743.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43296	9.1e-156	73.50	Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1	[more]
Q9SUL1	5.2e-151	69.62	Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S...	[more]
P43295	1.5e-150	70.83	Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S...	[more]
P25804	1.0e-146	67.20	Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
Q10716	1.8e-140	69.77	Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1	[more]

BLAST of HG10005743.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BIU9	7.9e-195	88.68	cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103490359 PE=3 SV=1	[more]
A0A0A0K2B5	1.8e-194	87.76	Papain-like cysteine proteinase isoform I OS=Cucumis sativus OX=3659 GN=Csa_7G00...	[more]
A0A5A7SS65	4.5e-190	87.63	Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A6J1I0L8	4.4e-185	85.41	probable cysteine protease RD19B OS=Cucurbita maxima OX=3661 GN=LOC111469766 PE=...	[more]
A0A6J1F4R7	7.4e-185	86.02	probable cysteine protease RD19B OS=Cucurbita moschata OX=3662 GN=LOC111440857 P...	[more]

BLAST of HG10005743.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39090.1	6.5e-157	73.50	Papain family cysteine protease	[more]
AT4G16190.1	3.7e-152	69.62	Papain family cysteine protease	[more]
AT2G21430.1	1.1e-151	70.83	Papain family cysteine protease	[more]
AT3G54940.2	2.1e-115	57.55	Papain family cysteine protease	[more]
AT3G19390.1	8.7e-61	42.39	Granulin repeat cysteine protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 329..335 score: 75.14 coord: 307..317 score: 58.8 coord: 158..173 score: 64.12
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 140..365 e-value: 1.3E-109 score: 380.1
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 140..363 e-value: 4.8E-74 score: 249.1
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 55..111 e-value: 7.9E-19 score: 78.5
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 55..111 e-value: 2.5E-10 score: 40.7
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 25..365 e-value: 1.2E-96 score: 326.2
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 29..366
None	No IPR available	PANTHER	PTHR12411:SF783	CYSTEINE PROTEASE RD19C-RELATED	coord: 29..366
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 158..169
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 329..348
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 305..315
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 141..364 e-value: 3.53596E-106 score: 309.169
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 53..364

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10005743	HG10005743	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10005743.1-cds	HG10005743.1-cds-Chr07:5313313..5313617	CDS
HG10005743.1-cds	HG10005743.1-cds-Chr07:5314015..5314261	CDS
HG10005743.1-cds	HG10005743.1-cds-Chr07:5315162..5315281	CDS
HG10005743.1-cds	HG10005743.1-cds-Chr07:5316190..5316663	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10005743.1	HG10005743.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0008234	cysteine-type peptidase activity
molecular_function	GO:0004197	cysteine-type endopeptidase activity