HG10003049.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10003049.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	beta-amyrin 11-oxidase-like
Location	Chr11: 16757209 .. 16760685 (-)
Sequence length	723
RNA-Seq Expression	HG10003049.1
Synteny	HG10003049.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTGAAGAATTGTTTGGTGTTGATATTTGCAGCCTTAGTTTTAGTTTATGGAGTTTTGAAGAGATTGAATGATTGGTATTATGAAGCCAAAGTGGGGAAATTATGGCCTAAGCTTCCTCCAGGTCATATGGGTTGGCCTCTTCTTGGTTCTACTCTATCTTACCTCAAGAATTTCAGCTCTGGTCAACCAAGAAATGTCATTCACAACCTTTCCATTAGGTAAATCTTTTATCATTAATATAATCTTACAATATATAATATGCGTAATTTCTTGTAGCTTCTTTCATGATCATACTTATTTTATACATACAAAATATATACCTCAAAACTTTGCTTTTTGTTTAAAAAATAACTTCGTTTTTTCAAAAGTTATAATGTTAGTCTTTGATGATCTTTTCATATATGTTTCAATAAAAGTAGAAACTTCTACTATTGTGTTGATGCCTAAATCTGGATTGGTAAAGTAGTCTAATCTTTATGTGGCAACAATAGTAAATTTGTATTTGAGAGAAAGAAGGTAAACTGCAAGTTAGCTAGAACTACTGATGTTAGGGGTGTTCAAATGACCTGACAATGCGAACAATTCGGACTGAGTCTCGGGTTGAATAAAAAAAAATTTAAAATTGAGTTCACCCAACCCAAATTACATATATATTAATAAGTTTAATTTTAGAGTTCAGCTTTAGGTTGAATTTTTTAATATTTTTCTATATTTCGATGTTTGTCTTTATTTAAAGTTTATAATAGATATCTGGTATTTAACATTGGAGTTTTATAAGATTTTAGTTATTAGTCATGTGTTGTTGGTAGATTTAAGTATTTTAAATTAATAAGAAAAAAATAACTCGACAATCGAGGGTTGAGTTTCGTTGGAGACCCTGTTCAGGTTGGTCTAAGAAAACCCCCCAACCTATGTATACATCCGTAACTGGTGTGGTGTAGTCTAAGAAAACCCCCCAACCTATGTATACATCCGTAACTGGTGTGGTGTAGGCTACATACACTCTGATGTTTAAGTCATGAAGTCTTAAAATAAGGTTCATTATAGAATTTCTAGAAGAGAGTCATCTTACCTCTTATATCATCTTCTTTGCGTATTTATAGGGTCAGATCGCCTGGATCTTCTCCAATTAGGGCTTTCTTAGCTTGTTTCTCACAAATTCCACTCTTTCCCAATTTGGCCAAATCATGTGAAGAATCACCTGACTTGATGGGCTTGGCCTCAACCTTGGACGAGGCCAAGCATGTTGATTTGGCTCTGGGCCAATTTAGCCCTTCTCTTTATGTCTCATGTTAGGCCGAATGTTGCAACTTTGCATGATTTGGTACGCTCGATTGAGGCCAAGAGAGCTTATTTGGTTTTGGGCCAAGTCAGTCCTTCCTTCTAGGCTCATTCCATCATTTGGACCTTATGTTGATCCATCTAACGTTATTTCCTTGGTCCAATTGCCTCTGCTTGTGCACTATGTCAATTTTACCTTTTAGTCCAAAATTATTAAAAACATATTGAATGAAAATTGAGATCTAAAACGGGTATGACATTGTTTACGGCTCCGATTGTCGTTCCAAGTTGTCACTGTGACACACTATTTTCAAGTCTCAATTTGCACCCAACATTGTAATGGATTATTATACTCTAAAGTTTAAAGAGATTTTTGCGTAATTAGCCTTCATTGACAAATAATAACTATTTTGAAACTACTCTCTTAAATAGAGAGATTAATATAGCCATCCAAATTTCAATGAATGTCAACTAATTCGTTCGAGATGTCGTAGCCTTGCTTCATCATAATTTTATACTCTGGAAATACCCTTGAACGTTTCTTTTTATTTAAAAATATCTTCATCCTTTCCAAAGTCATGATATTACTCTTGACTTTTTTTTATAAACGTTTCAAAAAAAATGTCTTGAATTGAACATTGAGACTTAAAATCACGTAAGGTATTATTTGGATCCTAATTATCATTCCAGATCATTGACCCACCATCTCCAATTCTCAATTTTCGTCAAATATTCTAACAAAATTACTATTCCAAAGTTCAGGAGTATTTTGTGTAATTTAGCCTTCATTGACAAATAATAGTTACTTTCGAAACTACTCTCTTAGATGGAGAGATTAAGCATCTAAATTTCAATGAACGTCACCTAATTCGTTCAAGACATCATAGTCTTGTGTAAAAGTATCTTTATTCTTTCAAAAGTTATGATATTACTCTTGATTTTTTTTTATAAAGGTTTAAAAAAAAGTACGTAAAAACTCATATTACAATGTCTTGAATTGAACATTGAGACTTAAAACAAATATGGTAGTGTCTGCATCCTCATTTTCATTTTAGATAATTGTCACACCATTCCCAATTCTCAATTTTAGTCAACTACTCTAACGGAAGTACTACTCCAAAGTTCAAGAATATTTTGTGTAATTTAGCATTCATGGACAAATAATAGTTACTTTGGAAACTACTCCCTTATTAAAATGCCTTGAATCTTTTATCTAGCGCTTCGAATAACCAAACACCGGTCTAATCTCTTAAGACTTTTTTTTTTTCTGAGGCAGATATGGGAAAAGTGATATGTACAAAGCTCACCTACTTGGTAGAGCGAGTATAATTGTTTGTACATCTGAACTTTGTCGTCAAGTTTTAAGTGATGAAGACAAATTCAAGCCTAGTTTACCCAGAAGCATCACCATTTTATCTGGTAGAAAGTCACTGATGCAAGTGTTCAAGGGGGAGCACAGGCGGCTACGGCGGCTGACAACGGCGCCGATCAGCGGCCATGTGGCACTAGAGATGTACATCAATCATATTGAAAACACAGTGATAAGTGGTTTAGAGGAATGGGCTAGCATGAAGAGGCCAATTGAGCTATTGACGGAGATAAAGAAGCTGACCTTCAAAATTATTTGGAACATTTTCATGGGTTCTTCTGTACATTCTTCCATTGCAGAAATAGAGGCTTTGTTTGCTAAAGTTGCAGTAGGGTTTATATCTTTGCCCATCAACTTTCCTGGCTTCAGCTTCCATAAATCACTCAAGGTAACCCTTTTTCTTCTATATGCATTCCTGTTAACTTTTCTTTTTTCTTTCAATAATTCATGTGTATGGTAGAAACAGTTAAAAAAAGAATGAATTTATCGTAATCTATCTAACTTACATATTAGTGATTTAATTAGAATTAATTTAATAATTAATTCATATATATGGTAACAAATTAAATAGCACAATTATATTTGGACTATTTAATGATCATATTGTGTAATCTTAATTGTGGAGTCCATAACGTTCCATATAGAAGCGTGACCTGTTGAGTAGAATCACTCCATTAATCTTAGGAGATAATGGAAAACCCTAAGATCAACTTATAAATATAAGTTGGTTCTAGTCTCCCTAATATGCTATGTGTCATGGGGCGGAAACTTGGTATGGAATTTTTTGAAATTTTCCCGGGCAGCTTAAGGAGGTTCCTTAG

mRNA sequence

ATGGAGTTGAAGAATTGTTTGGTGTTGATATTTGCAGCCTTAGTTTTAGTTTATGGAGTTTTGAAGAGATTGAATGATTGGTATTATGAAGCCAAAGTGGGGAAATTATGGCCTAAGCTTCCTCCAGGTCATATGGGTTGGCCTCTTCTTGGTTCTACTCTATCTTACCTCAAGAATTTCAGCTCTGGTCAACCAAGAAATGTCATTCACAACCTTTCCATTAGATATGGGAAAAGTGATATGTACAAAGCTCACCTACTTGGTAGAGCGAGTATAATTGTTTGTACATCTGAACTTTGTCGTCAAGTTTTAAGTGATGAAGACAAATTCAAGCCTAGTTTACCCAGAAGCATCACCATTTTATCTGGTAGAAAGTCACTGATGCAAGTGTTCAAGGGGGAGCACAGGCGGCTACGGCGGCTGACAACGGCGCCGATCAGCGGCCATGTGGCACTAGAGATGTACATCAATCATATTGAAAACACAGTGATAAGTGGTTTAGAGGAATGGGCTAGCATGAAGAGGCCAATTGAGCTATTGACGGAGATAAAGAAGCTGACCTTCAAAATTATTTGGAACATTTTCATGGGTTCTTCTGTACATTCTTCCATTGCAGAAATAGAGGCTTTGTTTGCTAAAGTTGCAGTAGGGTTTATATCTTTGCCCATCAACTTTCCTGGCTTCAGCTTCCATAAATCACTCAAGCTTAAGGAGGTTCCTTAG

Coding sequence (CDS)

ATGGAGTTGAAGAATTGTTTGGTGTTGATATTTGCAGCCTTAGTTTTAGTTTATGGAGTTTTGAAGAGATTGAATGATTGGTATTATGAAGCCAAAGTGGGGAAATTATGGCCTAAGCTTCCTCCAGGTCATATGGGTTGGCCTCTTCTTGGTTCTACTCTATCTTACCTCAAGAATTTCAGCTCTGGTCAACCAAGAAATGTCATTCACAACCTTTCCATTAGATATGGGAAAAGTGATATGTACAAAGCTCACCTACTTGGTAGAGCGAGTATAATTGTTTGTACATCTGAACTTTGTCGTCAAGTTTTAAGTGATGAAGACAAATTCAAGCCTAGTTTACCCAGAAGCATCACCATTTTATCTGGTAGAAAGTCACTGATGCAAGTGTTCAAGGGGGAGCACAGGCGGCTACGGCGGCTGACAACGGCGCCGATCAGCGGCCATGTGGCACTAGAGATGTACATCAATCATATTGAAAACACAGTGATAAGTGGTTTAGAGGAATGGGCTAGCATGAAGAGGCCAATTGAGCTATTGACGGAGATAAAGAAGCTGACCTTCAAAATTATTTGGAACATTTTCATGGGTTCTTCTGTACATTCTTCCATTGCAGAAATAGAGGCTTTGTTTGCTAAAGTTGCAGTAGGGTTTATATCTTTGCCCATCAACTTTCCTGGCTTCAGCTTCCATAAATCACTCAAGCTTAAGGAGGTTCCTTAG

Protein sequence

MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKEVP

Homology

[Close]

BLAST of HG10003049.1 vs. NCBI nr
Match: XP_008448712.1 (PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 411.8 bits (1057), Expect = 4.2e-111
Identity = 201/239 (84.10%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLN WYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           +SGQPR ++HNLSIRYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT+
Sbjct: 61  NSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           TEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLK ++
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARK 239

[Close]

BLAST of HG10003049.1 vs. NCBI nr
Match: XP_008448713.1 (PREDICTED: beta-amyrin 11-oxidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 411.8 bits (1057), Expect = 4.2e-111
Identity = 201/239 (84.10%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLN WYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           +SGQPR ++HNLSIRYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT+
Sbjct: 61  NSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           TEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLK ++
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARK 239

[Close]

BLAST of HG10003049.1 vs. NCBI nr
Match: XP_011650352.2 (beta-amyrin 11-oxidase [Cucumis sativus] >KAE8650035.1 hypothetical protein Csa_011096 [Cucumis sativus])

HSP 1 Score: 409.8 bits (1052), Expect = 1.6e-110
Identity = 202/239 (84.52%), Postives = 218/239 (91.21%), Query Frame = 0


Query: 1   MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI   L L++GV+KRLN WYYEAK+GKLWPKLPPG MGWP+LG TLSYLKNF
Sbjct: 1   MELKEWLVLITGGLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGVTLSYLKNF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           SSGQPR ++HNLSIRYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT+
Sbjct: 61  SSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLTTAPISGH ALEMYINHIE TVISGLEEWASMKRPIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTTAPISGHSALEMYINHIEQTVISGLEEWASMKRPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           TEIKKLTFKIIWNIFMG SS+ SSI EIEALFAK+ +GFISLPIN PGFSF+KSLK ++
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAKITLGFISLPINLPGFSFYKSLKARK 239

[Close]

BLAST of HG10003049.1 vs. NCBI nr
Match: XP_038905890.1 (beta-amyrin 11-oxidase-like [Benincasa hispida] >XP_038905891.1 beta-amyrin 11-oxidase-like [Benincasa hispida])

HSP 1 Score: 409.5 bits (1051), Expect = 2.1e-110
Identity = 204/236 (86.44%), Postives = 217/236 (91.95%), Query Frame = 0


Query: 1   MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELKN LV I AALV V+GVLKRLN WYYEAK+GKLWPKLPPG MGWPLLGSTLSYLK+F
Sbjct: 1   MELKNWLVWIIAALVFVFGVLKRLNGWYYEAKLGKLWPKLPPGDMGWPLLGSTLSYLKHF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           +SG PRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVL DEDKFKPSLPRSIT+
Sbjct: 61  TSGHPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLCDEDKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLTT  ISGH+ LEMYIN+I++TVI+GLEEWASMK+PIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTTGLISGHMVLEMYINYIQHTVINGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLK 236
           TEIKKLTFKIIWNIFMG SS+ SSI EIE LF K A+GFISLPINFPGF FHKSLK
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEPLFTKTALGFISLPINFPGFIFHKSLK 236

[Close]

BLAST of HG10003049.1 vs. NCBI nr
Match: KAA0053060.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa] >TYK11515.1 beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 407.1 bits (1045), Expect = 1.0e-109
Identity = 201/240 (83.75%), Postives = 221/240 (92.08%), Query Frame = 0


Query: 1   MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLN WYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSI-RYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSIT 120
           +SGQPR ++HNLSI RYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT
Sbjct: 61  NSGQPRILLHNLSISRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSIT 120

Query: 121 ILSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIEL 180
           +LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIEL
Sbjct: 121 LLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIEL 180

Query: 181 LTEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           LTEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLK ++
Sbjct: 181 LTEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARK 240

[Close]

BLAST of HG10003049.1 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 7.2e-58
Identity = 106/218 (48.62%), Postives = 151/218 (69.27%), Query Frame = 0


Query: 21  LKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKSD 80
           ++ LN WYY+ K+ +    LPPG MGWPL+G  LS++K+FSSG P + I+NL ++YG+S 
Sbjct: 24  VRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFINNLVLKYGRSG 83

Query: 81  MYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLMQVFKGEHRRLRR 140
           +YK HL G  SIIVC  ++CR+VL+D+  FK   P+SI  L+  + ++ V   EHR  RR
Sbjct: 84  IYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDVSNAEHRLFRR 143

Query: 141 LTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSSV 200
           L T+PI GH AL MY+  +E  VI+ LEE +SMK P+ELL E+KK++FK I ++FMGSS 
Sbjct: 144 LITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAIVHVFMGSSN 203

Query: 201 HSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
              I +I + F  +  G  S+PIN PGF+FHK+L+ ++
Sbjct: 204 QDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARK 241

[Close]

BLAST of HG10003049.1 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 6.1e-49
Identity = 93/219 (42.47%), Postives = 141/219 (64.38%), Query Frame = 0


Query: 20  VLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKS 79
           +++++N W YE+ +G+    LPPG +GWP +G+ LS+L+ F +  P +    L  RYG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 80  DMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLMQVFKGEHRRLR 139
            +YKAH+ G  SIIV TS+ CR+VL+D+D FKP  P S   L GRKS + +   EH+RLR
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 140 RLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSS 199
           RLT AP++GH AL  YI +IE  VI+ L++W  M    E LT ++KLTF+II  IF+ S 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 200 VHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
             + +  +E  +  +  G  ++ +N PGF++H++LK ++
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARK 242

[Close]

BLAST of HG10003049.1 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 5.2e-48
Identity = 95/230 (41.30%), Postives = 147/230 (63.91%), Query Frame = 0


Query: 9   LIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNV 68
           LI   L ++  VLKR+N W Y +K+G+    LPPG +GWP++G+  S+L+ F +  P + 
Sbjct: 13  LIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESF 72

Query: 69  IHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLM 128
           I +   RYG++ +YKAH+ G   ++V T E CR+VL+D+D F    P+S   L GRKS +
Sbjct: 73  IQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFV 132

Query: 129 QVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTF 188
            +   EH+RLRRLT+AP++G  AL +YI  IE TV + LE+W+ M   IE L+ ++KLTF
Sbjct: 133 GISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTF 192

Query: 189 KIIWNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           K+I  IF+ S     +  +E  +  +  G  ++ IN PGF++H++LK ++
Sbjct: 193 KVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARK 241

[Close]

BLAST of HG10003049.1 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.1e-41
Identity = 84/226 (37.17%), Postives = 138/226 (61.06%), Query Frame = 0


Query: 13  ALVLVYGVLKRLNDWYYEAKVGKL-WPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHN 72
           ALV+V    +R + WY EA +G     +LPPG MGWPL+G   ++L+ F SG+P   I +
Sbjct: 44  ALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGKPDAFIAS 103

Query: 73  LSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLMQVF 132
              R+G++ +Y++ +    +++V T+E C+QVL D+D F    P++   L G +S + + 
Sbjct: 104 FVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMP 163

Query: 133 KGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTFKII 192
             EHRR+R+LT API+G  AL  Y+  I+ TV S L  WA     +E LTE++++TFKII
Sbjct: 164 YDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKII 223

Query: 193 WNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLK 238
             IF+G +  ++   +E  + ++  G  ++ IN PGF++  +L+ +
Sbjct: 224 VQIFLGGADQATTRALERSYTELNYGMRAMAINLPGFAYRGALRAR 269

[Close]

BLAST of HG10003049.1 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.8e-41
Identity = 84/233 (36.05%), Postives = 143/233 (61.37%), Query Frame = 0


Query: 8   VLIFAALVLVYGVLKRLNDWYYEAKVGK-LWPKLPPGHMGWPLLGSTLSYLKNFSSGQPR 67
           V+   A+V +   ++  + WY+ A +G     +LPPG MGWPL+G   ++L+ F SG+P 
Sbjct: 12  VVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRPD 71

Query: 68  NVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKS 127
           + I + + R+G++ +Y+A +    +I+  T E C+QVL D+D F    P++   L G KS
Sbjct: 72  SFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKS 131

Query: 128 LMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASM--KRPIELLTEIK 187
            + +   EHRRLR+LT API+G  AL  Y+  I++TV++ L  W+        E LTE++
Sbjct: 132 FVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELR 191

Query: 188 KLTFKIIWNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLK 238
           ++TF+II  IFMG +   + AE+E  + ++  G  ++ I+ PGF++HK+++ +
Sbjct: 192 RMTFRIIVQIFMGGADERTAAELERTYTELNYGMRAMAIDLPGFAYHKAIRAR 244

[Close]

BLAST of HG10003049.1 vs. ExPASy TrEMBL
Match: A0A1S3BJR1 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=3 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 2.0e-111
Identity = 201/239 (84.10%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLN WYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           +SGQPR ++HNLSIRYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT+
Sbjct: 61  NSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           TEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLK ++
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARK 239

[Close]

BLAST of HG10003049.1 vs. ExPASy TrEMBL
Match: A0A1S3BKD0 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=4 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 2.0e-111
Identity = 201/239 (84.10%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLN WYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           +SGQPR ++HNLSIRYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT+
Sbjct: 61  NSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           TEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLK ++
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARK 239

[Close]

BLAST of HG10003049.1 vs. ExPASy TrEMBL
Match: A0A5D3CIW4 (Beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001790 PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 5.0e-110
Identity = 201/240 (83.75%), Postives = 221/240 (92.08%), Query Frame = 0


Query: 1   MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           MELK  LVLI  +L L++GV+KRLN WYYEAK+GKLWPKLPPG MGWP+LGSTLS+LKNF
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SSGQPRNVIHNLSI-RYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSIT 120
           +SGQPR ++HNLSI RYGKSDMYK HLLGRASIIVCTSE+CRQVL DE+KFKPSLPRSIT
Sbjct: 61  NSGQPRILLHNLSISRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSIT 120

Query: 121 ILSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIEL 180
           +LSGRKSLMQVFK EHRRLRRLT APISGH ALEMYINHIE TVISGLEEWASMK+PIEL
Sbjct: 121 LLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIEL 180

Query: 181 LTEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           LTEIKKLTFKIIWNIFMG SS+ SSI EIEALFA++A+GFISLPINFPGFSFHKSLK ++
Sbjct: 181 LTEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARK 240

[Close]

BLAST of HG10003049.1 vs. ExPASy TrEMBL
Match: A0A6J1L1N7 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500298 PE=3 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.4e-101
Identity = 184/239 (76.99%), Postives = 209/239 (87.45%), Query Frame = 0


Query: 1   MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           ME+KN LVLI AA V V G+LKRLN WYY AK+GK WPKLPPG MGWPLLGST+SY+KNF
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNF 63

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
            SGQP N I N  IRYGK DMYK HLLGRA+IIVC+SELCRQVL DED FKPSLP++I +
Sbjct: 64  ISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           L+GRKSL+QVFK EH+RLRRLT APISGH ALEMYINHIE+TV+SGLEEWASMK PIELL
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKIIWNIFMG-SSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           +EIKKLTFKIIWNIFMG SS+ SSI E+E LF++VA+GFISLPINFPGF+ HK+LK ++
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARK 242

[Close]

BLAST of HG10003049.1 vs. ExPASy TrEMBL
Match: A0A6J1L8N2 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500299 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.1e-98
Identity = 179/238 (75.21%), Postives = 205/238 (86.13%), Query Frame = 0


Query: 1   MELKNCLVLIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNF 60
           ME+KN LVLI AA V V G+LKRLN WYY AK+GK WPKLPPGHMGWPLLGST+ +LK+F
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGHMGWPLLGSTILFLKSF 63

Query: 61  SSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITI 120
           SSGQPRN I +  IRYGK DMYK H+LGRA+IIVCTSELCRQVL DE  FKPS P SI  
Sbjct: 64  SSGQPRNFIRSFYIRYGKCDMYKTHILGRATIIVCTSELCRQVLGDEHNFKPSFPVSINR 123

Query: 121 LSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELL 180
           L+GRKSL+QVFK EH+RLRRLTTAPISGH AL+MYIN IE+TV+SGLEEWASMK PIELL
Sbjct: 124 LTGRKSLIQVFKAEHKRLRRLTTAPISGHAALKMYINGIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKIIWNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           +E+KKLTFKIIWNIFMGSS+  SI E+  LF++VA+GFISLPIN PGF+FH+SLK ++
Sbjct: 184 SEMKKLTFKIIWNIFMGSSLEYSIDEMVVLFSQVAIGFISLPINSPGFNFHQSLKARK 241

[Close]

BLAST of HG10003049.1 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 195.7 bits (496), Expect = 4.4e-50
Identity = 93/219 (42.47%), Postives = 141/219 (64.38%), Query Frame = 0


Query: 20  VLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKS 79
           +++++N W YE+ +G+    LPPG +GWP +G+ LS+L+ F +  P +    L  RYG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 80  DMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLMQVFKGEHRRLR 139
            +YKAH+ G  SIIV TS+ CR+VL+D+D FKP  P S   L GRKS + +   EH+RLR
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 140 RLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSS 199
           RLT AP++GH AL  YI +IE  VI+ L++W  M    E LT ++KLTF+II  IF+ S 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 200 VHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
             + +  +E  +  +  G  ++ +N PGF++H++LK ++
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARK 242

[Close]

BLAST of HG10003049.1 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 192.6 bits (488), Expect = 3.7e-49
Identity = 95/230 (41.30%), Postives = 147/230 (63.91%), Query Frame = 0


Query: 9   LIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNV 68
           LI   L ++  VLKR+N W Y +K+G+    LPPG +GWP++G+  S+L+ F +  P + 
Sbjct: 13  LIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESF 72

Query: 69  IHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLM 128
           I +   RYG++ +YKAH+ G   ++V T E CR+VL+D+D F    P+S   L GRKS +
Sbjct: 73  IQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFV 132

Query: 129 QVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTF 188
            +   EH+RLRRLT+AP++G  AL +YI  IE TV + LE+W+ M   IE L+ ++KLTF
Sbjct: 133 GISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTF 192

Query: 189 KIIWNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           K+I  IF+ S     +  +E  +  +  G  ++ IN PGF++H++LK ++
Sbjct: 193 KVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARK 241

[Close]

BLAST of HG10003049.1 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 192.6 bits (488), Expect = 3.7e-49
Identity = 95/230 (41.30%), Postives = 147/230 (63.91%), Query Frame = 0


Query: 9   LIFAALVLVYGVLKRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNV 68
           LI   L ++  VLKR+N W Y +K+G+    LPPG +GWP++G+  S+L+ F +  P + 
Sbjct: 13  LIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESF 72

Query: 69  IHNLSIRYGKSDMYKAHLLGRASIIVCTSELCRQVLSDEDKFKPSLPRSITILSGRKSLM 128
           I +   RYG++ +YKAH+ G   ++V T E CR+VL+D+D F    P+S   L GRKS +
Sbjct: 73  IQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFV 132

Query: 129 QVFKGEHRRLRRLTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTF 188
            +   EH+RLRRLT+AP++G  AL +YI  IE TV + LE+W+ M   IE L+ ++KLTF
Sbjct: 133 GISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTF 192

Query: 189 KIIWNIFMGSSVHSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
           K+I  IF+ S     +  +E  +  +  G  ++ IN PGF++H++LK ++
Sbjct: 193 KVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARK 241

[Close]

BLAST of HG10003049.1 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 108.6 bits (270), Expect = 7.0e-24
Identity = 60/218 (27.52%), Postives = 112/218 (51.38%), Query Frame = 0


Query: 22  KRLNDWYYEAKVGKLWPKLPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKSDM 81
           K+LND +  ++     PK P G +GWP++G T+ ++ +  S +P + +    + YG+  +
Sbjct: 35  KKLNDHHVTSQ--SHGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGR--V 94

Query: 82  YKAHLLGRASIIVCTSELCRQVL-SDEDKFKPSLPRSITILSGRKSLMQVFKGEHRRLRR 141
           +K+H+ G A+I+   +E+ R VL SD   F P  P+++  L G+ S++ +    HRR   
Sbjct: 95  FKSHIFGTATIVSTDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHG 154

Query: 142 LTTAPISGHVALEMYINHIENTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSSV 201
           L  + +   +     +  +   +   ++ W S  +P+ L    K + FK++    +    
Sbjct: 155 LVGSFLKSPLLKAQIVRDMHKFLSESMDLW-SEDQPVLLQDVSKTVAFKVLAKALISVEK 214

Query: 202 HSSIAEIEALFAKVAVGFISLPINFPGFSFHKSLKLKE 239
              + E++  F     G +SLPINFPG   H+SL+ K+
Sbjct: 215 GEDLEELKREFENFISGLMSLPINFPGTQLHRSLQAKK 247

[Close]

BLAST of HG10003049.1 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.9e-22
Identity = 62/200 (31.00%), Postives = 105/200 (52.50%), Query Frame = 0


Query: 40  LPPGHMGWPLLGSTLSYLKNFSSGQPRNVIHNLSIRYGKSDMYKAHLLGRASIIVCTSEL 99
           +P G +GWP++G TL+++    S +P   +      YGK  ++K +++G   II   +E+
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGK--VFKTNIIGTPIIISTDAEV 128

Query: 100 CRQVLSDE-DKFKPSLPRSITILSGRKSLMQVFKGEHRRLRRLTTAPISGHVALEMYINH 159
            + VL +  + F P+ P+SIT L G  S++ +     +RL  L  A +      +     
Sbjct: 129 NKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRD 188

Query: 160 IENTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSSVHSSIAEIEALFAKVAVGF 219
           IE +V+  L  WA +   + +  EIKK+TF+I+  + M +S    +  ++  F +   G 
Sbjct: 189 IEASVVLTLASWAQLPL-VHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGL 248

Query: 220 ISLPINFPGFSFHKSLKLKE 239
           I +PI FPG   +KSLK KE
Sbjct: 249 ICIPIKFPGTRLYKSLKAKE 265

The following BLAST results are available for this feature:

BLAST of HG10003049.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008448712.1	4.2e-111	84.10	PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo]	[more]
XP_008448713.1	4.2e-111	84.10	PREDICTED: beta-amyrin 11-oxidase-like isoform X2 [Cucumis melo]	[more]
XP_011650352.2	1.6e-110	84.52	beta-amyrin 11-oxidase [Cucumis sativus] >KAE8650035.1 hypothetical protein Csa_...	[more]
XP_038905890.1	2.1e-110	86.44	beta-amyrin 11-oxidase-like [Benincasa hispida] >XP_038905891.1 beta-amyrin 11-o...	[more]
KAA0053060.1	1.0e-109	83.75	beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa] >TYK11515.1 be...	[more]

BLAST of HG10003049.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	7.2e-58	48.62	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
O23051	6.1e-49	42.47	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q9C5Y2	5.2e-48	41.30	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q43246	2.1e-41	37.17	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]
Q9AXH9	2.8e-41	36.05	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]

BLAST of HG10003049.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BJR1	2.0e-111	84.10	beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490801 P...	[more]
A0A1S3BKD0	2.0e-111	84.10	beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490801 P...	[more]
A0A5D3CIW4	5.0e-110	83.75	Beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1L1N7	1.4e-101	76.99	beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1115002...	[more]
A0A6J1L8N2	1.1e-98	75.21	beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1115002...	[more]

BLAST of HG10003049.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05160.1	4.4e-50	42.47	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G32440.1	3.7e-49	41.30	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	3.7e-49	41.30	ent-kaurenoic acid hydroxylase 2	[more]
AT3G13730.1	7.0e-24	27.52	cytochrome P450, family 90, subfamily D, polypeptide 1	[more]
AT4G36380.1	2.9e-22	31.00	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 41..201 e-value: 8.1E-9 score: 34.7
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 33..239 e-value: 5.1E-32 score: 113.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 38..226
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 18..237
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 18..237

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10003049	HG10003049	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10003049.1-cds	HG10003049.1-cds-Chr11:16757209..16757226	CDS
HG10003049.1-cds	HG10003049.1-cds-Chr11:16757642..16758122	CDS
HG10003049.1-cds	HG10003049.1-cds-Chr11:16760462..16760685	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10003049.1	HG10003049.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen