Cucsat.G8928.T5 (mRNA) Cucumber (B10) v3

BLAST of Cucsat.G8928.T5 vs. ExPASy Swiss-Prot
Match: F4IXE7 (Increased DNA methylation 1 OS=Arabidopsis thaliana OX=3702 GN=IDM1 PE=1 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.4e-198
Identity = 469/1274 (36.81%), Postives = 646/1274 (50.71%), Query Frame = 0

Query: 6    DGFEGSANEEIIFREVFFGNGSSHSNKRC-PHKAFGYEHGPCKINDASLCSSSEPSAVSI 65
            D FEGS  +  IFREVFFG+   ++ KRC    A  +E    K  ++SL S+S    V  
Sbjct: 13   DCFEGSYEDHQIFREVFFGSDPGNTTKRCLVTGAINFECDSSKNVNSSLSSNS----VVT 72

Query: 66   YSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASDSVPN 125
              Y+       C    E     SAS          +G D N   KR+K+S +   D+   
Sbjct: 73   SGYA-------CPQGFE----ASASR---------DGSDFNTKAKRVKLSGNKHLDA--- 132

Query: 126  LVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLV--EMDSN 185
                +    S     + +  P  +   E+   H+VESS +G+ +S Y L+  +    +  
Sbjct: 133  ----RDEKGS-----ALHGFPTSDIARETIPLHLVESSNKGVSTSSYLLKHSIVKGREVY 192

Query: 186  LADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLHLE 245
            L    + K  SLNL+         K  ASPVSQES  TR++    S   SEK   PL L 
Sbjct: 193  LGGIVSGKCKSLNLDKCDGKEF--KAIASPVSQESFATRMISVGASTPHSEKACFPLQLN 252

Query: 246  VGQMKSLCPELDAS---LKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPC-RRYMETV 305
             G  K    EL  S   LK D   DPRPLL+ +V  +  AA W IE+ +R   R++++T 
Sbjct: 253  NGS-KVSPNELIMSKTCLKIDPKEDPRPLLYKYVCKVLTAARWKIEKRERSAGRKHVDTF 312

Query: 306  YRSPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEM 365
            Y SP+GR FREF  AW+  G +L ADR   + D  +K+WTGI+ F  DLS TLL I + M
Sbjct: 313  YISPEGRKFREFGSAWKALGGILLADR--KLMDTGTKKWTGINDFWSDLSLTLLDIEENM 372

Query: 366  NQLGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLIN 425
              L    + A  W  L+P+VVVVFI +++G LR+G+                        
Sbjct: 373  KNLNLANTRALWWSALEPFVVVVFISKQVGSLRKGN------------------------ 432

Query: 426  EDNGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTE 485
                  K+   +N++P                                            
Sbjct: 433  ------KVEVARNSNP-------------------------------------------- 492

Query: 486  DGTTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDG 545
                                            +K++ ED                CL   
Sbjct: 493  --------------------------------DKLKKEDTI--------------CLN-- 552

Query: 546  PVPSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEV 605
             + SG  ++V+ +S      E S L    + + E H                        
Sbjct: 553  -LISGCPESVLTVS------EGSHLVHDVDANQEIH------------------------ 612

Query: 606  PLGKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAI 665
                  ++LE          S  L      G     +  +E      AS    ED     
Sbjct: 613  ------SDLEVQTKISSQKVSSRLERQSIIGKEISGTHEQEASKGIVASKLIAED---MH 672

Query: 666  HSILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIA 725
             S+++K   R+ KKIS+IKP    Q D +     N  EF D           E    H+ 
Sbjct: 673  ESVMRKNLHRRSKKISDIKPASLDQHDSLDSNSLNSFEFQD----------KEMGNIHLV 732

Query: 726  DTKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAI 785
               + D   +N  ++   C+S++   K  K++                  +DDDL+ S I
Sbjct: 733  SKGSRDERLRNEKMNNSCCNSKKGRKKARKHY-----------------TQDDDLMGSTI 792

Query: 786  IRNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGAR 845
             RNK   S +   S  +K  K +A+  +++ +  C+LL RS  + E ++  G W  LG R
Sbjct: 793  TRNKGKFSRS---SQKKKTQKPKARTKKRNNRGGCRLLPRSSSNVENHFFQGNWSILGPR 852

Query: 846  TVLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFK 905
            TVLSWL+   VIS +++IQ + P D +VVK G +T DG++C CC+  +S+SEFK+HAGF 
Sbjct: 853  TVLSWLIATKVISRDEVIQLRDPDDDTVVKTGLVTKDGVVCTCCNKTVSLSEFKNHAGFN 912

Query: 906  FNRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELI 965
             N  C NLF+ SG+PF  CQL+AWS EYK R++  R  +  +DD NDDSCG+CGDGGELI
Sbjct: 913  QNCPCLNLFMGSGKPFASCQLEAWSAEYKARRNGWRLEKASDDDPNDDSCGVCGDGGELI 972

Query: 966  CCDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYH 1025
            CCDNCPSTFH +CLS+Q LPEG+WYC +CTC IC +LV+  + +  S   KC QC  KYH
Sbjct: 973  CCDNCPSTFHQACLSMQVLPEGSWYCSSCTCWICSELVS--DNAERSQDFKCSQCAHKYH 1032

Query: 1026 GQCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKIL 1085
            G CL+       +    +FC  +C+K+Y  L S++G+ N  A+G SW++L+C   D  + 
Sbjct: 1033 GTCLQGISKRRKLFPETYFCGKNCEKVYNGLSSRVGIINPNADGLSWSILKCFQEDGMVH 1050

Query: 1086 STARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYT 1145
            S  RLA+ AECNS+L VAL+IMEE FLSMVDPRTGIDMIPH++Y+W S+F RLDF GFYT
Sbjct: 1093 SARRLALKAECNSKLAVALSIMEESFLSMVDPRTGIDMIPHVLYNWGSTFARLDFDGFYT 1050

Query: 1146 VILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLV 1205
            V++EKDDV++ VASIRVHG  +AEMPL+ATCSKYRRQGMCR L+ AIEEMLMS KV+KLV
Sbjct: 1153 VVVEKDDVMISVASIRVHGVTIAEMPLVATCSKYRRQGMCRILVAAIEEMLMSLKVEKLV 1050

Query: 1206 IAAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIH 1265
            +AA+PSLVETWTEGFGF  +++EE+ +L + NLMVFPGT LLKK LY S + + T  G+ 
Sbjct: 1213 VAALPSLVETWTEGFGFKPMDDEERDALKRINLMVFPGTTLLKKTLYESTKPS-TMKGVC 1050

Query: 1266 SGVQLDTDAKQQCD 1273
               + +  + ++ D
Sbjct: 1273 LSKERNNPSNKEAD 1050

BLAST of Cucsat.G8928.T5 vs. ExPASy Swiss-Prot
Match: Q14839 (Chromodomain-helicase-DNA-binding protein 4 OS=Homo sapiens OX=9606 GN=CHD4 PE=1 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 2.3e-10
Identity = 27/53 (50.94%), Postives = 37/53 (69.81%), Query Frame = 0

Query: 939 DEDDRNDDSCGICGDGGELICCDNCPSTFHHSCLS--IQELPEGNWYCLNCTC 990
           +EDD + + C +C DGGEL+CCD CPS++H  CL+  + E+P G W C  CTC
Sbjct: 443 EEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTC 495

BLAST of Cucsat.G8928.T5 vs. ExPASy Swiss-Prot
Match: Q6PDQ2 (Chromodomain-helicase-DNA-binding protein 4 OS=Mus musculus OX=10090 GN=Chd4 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.3e-10
Identity = 27/53 (50.94%), Postives = 37/53 (69.81%), Query Frame = 0

Query: 939 DEDDRNDDSCGICGDGGELICCDNCPSTFHHSCLS--IQELPEGNWYCLNCTC 990
           +EDD + + C +C DGGEL+CCD CPS++H  CL+  + E+P G W C  CTC
Sbjct: 436 EEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTC 488

BLAST of Cucsat.G8928.T5 vs. ExPASy Swiss-Prot
Match: O43918 (Autoimmune regulator OS=Homo sapiens OX=9606 GN=AIRE PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 3.9e-10
Identity = 26/47 (55.32%), Postives = 34/47 (72.34%), Query Frame = 0

Query: 943 RNDDSCGICGDGGELICCDNCPSTFHHSCLS--IQELPEGNWYCLNC 988
           +N+D C +C DGGELICCD CP  FH +CLS  ++E+P G W C +C
Sbjct: 294 KNEDECAVCRDGGELICCDGCPRAFHLACLSPPLREIPSGTWRCSSC 340

BLAST of Cucsat.G8928.T5 vs. ExPASy Swiss-Prot
Match: O97159 (Chromodomain-helicase-DNA-binding protein Mi-2 homolog OS=Drosophila melanogaster OX=7227 GN=Mi-2 PE=1 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 6.6e-10
Identity = 25/55 (45.45%), Postives = 39/55 (70.91%), Query Frame = 0

Query: 937 EVDEDDRNDDSCGICGDGGELICCDNCPSTFHHSCLS--IQELPEGNWYCLNCTC 990
           E ++DD + + C +C DGGEL+CCD+CPS +H  CL+  +  +P+G+W C  C+C
Sbjct: 429 EEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPRCSC 483

BLAST of Cucsat.G8928.T5 vs. NCBI nr
Match: XP_004146095.1 (increased DNA methylation 1 [Cucumis sativus] >XP_031739517.1 increased DNA methylation 1 [Cucumis sativus] >XP_031739518.1 increased DNA methylation 1 [Cucumis sativus] >KAE8649828.1 hypothetical protein Csa_012099 [Cucumis sativus])

HSP 1 Score: 2805 bits (7270), Expect = 0.0
Identity = 1388/1393 (99.64%), Postives = 1389/1393 (99.71%), Query Frame = 0

Query: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
            MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS
Sbjct: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60

Query: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
            AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTD ASD
Sbjct: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDEASD 120

Query: 121  SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
            SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD
Sbjct: 121  SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180

Query: 181  SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
            SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH
Sbjct: 181  SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240

Query: 241  LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
            LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241  LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300

Query: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
            SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ
Sbjct: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360

Query: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
            LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED
Sbjct: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420

Query: 421  NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
            NGF KLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG
Sbjct: 421  NGFRKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480

Query: 481  TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
            TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV
Sbjct: 481  TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540

Query: 541  PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
            PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKN LTCSLVEGKKLEVPL
Sbjct: 541  PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNVLTCSLVEGKKLEVPL 600

Query: 601  GKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIHS 660
            GKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIHS
Sbjct: 601  GKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIHS 660

Query: 661  ILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIADT 720
            ILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIADT
Sbjct: 661  ILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIADT 720

Query: 721  KNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAIIR 780
            KNVDSHEKNLSLSPISCHSERKGSKL KNFDSHKGSKTRKKKLNECQIEDDDLLVSAIIR
Sbjct: 721  KNVDSHEKNLSLSPISCHSERKGSKLKKNFDSHKGSKTRKKKLNECQIEDDDLLVSAIIR 780

Query: 781  NKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGARTV 840
            NKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGARTV
Sbjct: 781  NKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGARTV 840

Query: 841  LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKFN 900
            LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKFN
Sbjct: 841  LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKFN 900

Query: 901  RACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELICC 960
            RACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELICC
Sbjct: 901  RACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELICC 960

Query: 961  DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHGQ 1020
            DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHGQ
Sbjct: 961  DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHGQ 1020

Query: 1021 CLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILST 1080
            CLKQRDI+SGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILST
Sbjct: 1021 CLKQRDIDSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILST 1080

Query: 1081 ARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140
            ARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI
Sbjct: 1081 ARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140

Query: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200
            LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA
Sbjct: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200

Query: 1201 AIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIHSG 1260
            AIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIHSG
Sbjct: 1201 AIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIHSG 1260

Query: 1261 VQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLVES 1320
            VQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLVES
Sbjct: 1261 VQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLVES 1320

Query: 1321 NGLDISPGQKPVESNCCTDIVGATTETCTQEAKKLLKVEVGVERDIQLSEGKSWDEGVHG 1380
            NGLDISPGQKPVESNCCTDIVGATTETCTQEAKKLLKVEVGVERDIQLSEGKSWDEGVHG
Sbjct: 1321 NGLDISPGQKPVESNCCTDIVGATTETCTQEAKKLLKVEVGVERDIQLSEGKSWDEGVHG 1380

Query: 1381 ATMTRFVEPVVLT 1393
            ATMTRFVEPVVLT
Sbjct: 1381 ATMTRFVEPVVLT 1393

BLAST of Cucsat.G8928.T5 vs. NCBI nr
Match: XP_016903085.1 (PREDICTED: increased DNA methylation 1 isoform X1 [Cucumis melo] >XP_016903087.1 PREDICTED: increased DNA methylation 1 isoform X2 [Cucumis melo])

HSP 1 Score: 2598 bits (6735), Expect = 0.0
Identity = 1299/1393 (93.25%), Postives = 1321/1393 (94.83%), Query Frame = 0

Query: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
            MDFQDDGFEGSANEEIIFRE+FFGNGSSHSNKRCPHKAF YEH PCKINDASLCSSSEPS
Sbjct: 1    MDFQDDGFEGSANEEIIFREIFFGNGSSHSNKRCPHKAFSYEHRPCKINDASLCSSSEPS 60

Query: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
             VS YSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTD ASD
Sbjct: 61   TVSSYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDEASD 120

Query: 121  SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
            SVPNLVKLKQSSDSIR PVSANC PAEECD ESFTFHIVESSRQGIISSCYRL+DL EMD
Sbjct: 121  SVPNLVKLKQSSDSIRVPVSANCYPAEECDSESFTFHIVESSRQGIISSCYRLKDLEEMD 180

Query: 181  SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
            SNL DPDAVK+TSLNLEG+ EPNMVNKVSASPVSQESSMTRLLVA+P D I+EKF SPLH
Sbjct: 181  SNLGDPDAVKRTSLNLEGNDEPNMVNKVSASPVSQESSMTRLLVASP-DTINEKFGSPLH 240

Query: 241  LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
            LEVGQMKSLCPEL ASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241  LEVGQMKSLCPELGASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300

Query: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
            SPQGRAFREFSKAWRFCGELLFADRCSFVKDV+SKEWTGIHQFLFDLSDTLL  GKEMNQ
Sbjct: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVDSKEWTGIHQFLFDLSDTLLQFGKEMNQ 360

Query: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
            LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGS KTD FVTL+NED
Sbjct: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSGKTDAFVTLVNED 420

Query: 421  NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
            N  C LSADKNASP+HDNSPSAKSALTEAPLKDLD GNCAFDEQTCDTS SNYYGHTEDG
Sbjct: 421  NSICNLSADKNASPLHDNSPSAKSALTEAPLKDLDGGNCAFDEQTCDTSLSNYYGHTEDG 480

Query: 481  TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
            TTKFPTRVSNY PNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFS STCKPRCL DGPV
Sbjct: 481  TTKFPTRVSNYDPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSGSTCKPRCLADGPV 540

Query: 541  PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
            PSGNSDNVVRISGL SPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL
Sbjct: 541  PSGNSDNVVRISGLTSPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600

Query: 601  GKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIHS 660
             KAENNLEESLNDC NYTSDGLSHSCASGVVQKSSQNEEGGL+FSASMFKTEDKVSAIHS
Sbjct: 601  SKAENNLEESLNDCANYTSDGLSHSCASGVVQKSSQNEEGGLNFSASMFKTEDKVSAIHS 660

Query: 661  ILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIADT 720
            ILKKKGRRKCKKISEIKP LPPQIDI              DG+CSQLDMIEDQKSHIADT
Sbjct: 661  ILKKKGRRKCKKISEIKPNLPPQIDI--------------DGSCSQLDMIEDQKSHIADT 720

Query: 721  KNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAIIR 780
            KNVDSHEKNLSLSPISCHSERK SKL KNFDS KGSKTRKKKLNECQIEDDDLLVSAIIR
Sbjct: 721  KNVDSHEKNLSLSPISCHSERKSSKLKKNFDSLKGSKTRKKKLNECQIEDDDLLVSAIIR 780

Query: 781  NKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGARTV 840
            NKDVSSSAAGFSHVRKY KSRAKMNRKSQKSSCKLLLRSLG+GEKNYKDGKWYALGARTV
Sbjct: 781  NKDVSSSAAGFSHVRKYLKSRAKMNRKSQKSSCKLLLRSLGNGEKNYKDGKWYALGARTV 840

Query: 841  LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKFN 900
            LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCC D+LSIS+FKSHAGFKFN
Sbjct: 841  LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCGDLLSISKFKSHAGFKFN 900

Query: 901  RACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELICC 960
            RAC NLFLDSGRPFMLCQLQAWSTEYKTRKS+TRTVEVDEDDRNDDSCGICGDGGELICC
Sbjct: 901  RACLNLFLDSGRPFMLCQLQAWSTEYKTRKSRTRTVEVDEDDRNDDSCGICGDGGELICC 960

Query: 961  DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHGQ 1020
            DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICG LVN+EEISSSSDALKCFQCEQKYHGQ
Sbjct: 961  DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGGLVNYEEISSSSDALKCFQCEQKYHGQ 1020

Query: 1021 CLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILST 1080
            CLKQRDINSGVESHIWFCS SCQKIY ALQS+LGLTNQFANGFSW LLRCIH DQKILST
Sbjct: 1021 CLKQRDINSGVESHIWFCSDSCQKIYTALQSRLGLTNQFANGFSWMLLRCIHNDQKILST 1080

Query: 1081 ARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140
             RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI
Sbjct: 1081 PRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140

Query: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200
            LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA
Sbjct: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200

Query: 1201 AIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIHSG 1260
            AIPSLVETWTEGFGF  VENEEKQSLH+FNLMVFPGTVLLKKALYVSGQTTETTVG    
Sbjct: 1201 AIPSLVETWTEGFGFAPVENEEKQSLHRFNLMVFPGTVLLKKALYVSGQTTETTVG---- 1260

Query: 1261 VQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLVES 1320
            VQLDT+ KQQC+S EPCPRMEMKCSKYQELQE NGEK KDDHEA+PAPP+DSSTLQLVES
Sbjct: 1261 VQLDTNTKQQCNSNEPCPRMEMKCSKYQELQEHNGEKTKDDHEANPAPPVDSSTLQLVES 1320

Query: 1321 NGLDISPGQKPVESNCCTDIVGATTETCTQEAKKLLKVEVGVERDIQLSEGKSWDEGVHG 1380
            NGLD SPGQK VESNCCTDIVGATTETC  EAKKLLKVEVGVE DIQLSEGKSWDEGVHG
Sbjct: 1321 NGLDTSPGQKFVESNCCTDIVGATTETCNHEAKKLLKVEVGVECDIQLSEGKSWDEGVHG 1374

Query: 1381 ATMTRFVEPVVLT 1393
            ATMTRFVEPVVLT
Sbjct: 1381 ATMTRFVEPVVLT 1374

BLAST of Cucsat.G8928.T5 vs. NCBI nr
Match: XP_038898710.1 (increased DNA methylation 1 [Benincasa hispida] >XP_038898711.1 increased DNA methylation 1 [Benincasa hispida] >XP_038898712.1 increased DNA methylation 1 [Benincasa hispida])

HSP 1 Score: 2399 bits (6217), Expect = 0.0
Identity = 1204/1401 (85.94%), Postives = 1269/1401 (90.58%), Query Frame = 0

Query: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
            MDFQDDGFEGS NEEIIF+EVFFGNGSS SN RCP K F YEHGPCKINDASLCSSSE S
Sbjct: 1    MDFQDDGFEGSVNEEIIFKEVFFGNGSSRSNMRCPRKTFSYEHGPCKINDASLCSSSELS 60

Query: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
             VS YSYSRN+KLDECYN TENI+T SA +SLPCK  SVEGD+ NAS KRIK+STD ASD
Sbjct: 61   TVSSYSYSRNIKLDECYNPTENIKTSSAPDSLPCKWTSVEGDNVNASAKRIKLSTDEASD 120

Query: 121  SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
            SVPNLVK+K+SSDS+R+PVS NC P E+CD ESFTFHIVESSRQGIISSCY L+  VE D
Sbjct: 121  SVPNLVKVKESSDSVRDPVSTNCCPVEDCDSESFTFHIVESSRQGIISSCYLLKHFVERD 180

Query: 181  SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
            SNL DPDA K+TSLNLEG+ EPNMVNKVSASPVSQESSMTRLLVA+P D ++EKF SPLH
Sbjct: 181  SNLGDPDA-KRTSLNLEGNDEPNMVNKVSASPVSQESSMTRLLVASP-DTLNEKFGSPLH 240

Query: 241  LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
            LEVGQMK  CPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241  LEVGQMKFPCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300

Query: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
            SPQGRAFREFSKAWR CGELLFADRCSFVK+V+SKEWTGIHQFLFDLSDTLL +GKEMNQ
Sbjct: 301  SPQGRAFREFSKAWRACGELLFADRCSFVKEVDSKEWTGIHQFLFDLSDTLLKVGKEMNQ 360

Query: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
            LGATTSLA CWVILDPYVVVVFI RKIG LRRGD VRATCS+G+NG++KT+ FVTL NED
Sbjct: 361  LGATTSLAQCWVILDPYVVVVFIGRKIGTLRRGDSVRATCSIGVNGNNKTEAFVTLTNED 420

Query: 421  NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
            N  C LSADKNASP+HDNSPSAKSALTEA LKDLD GNCAFDEQTCDTSFS+YYGHTEDG
Sbjct: 421  NSICNLSADKNASPLHDNSPSAKSALTEAALKDLDGGNCAFDEQTCDTSFSHYYGHTEDG 480

Query: 481  TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
            T KF TRVSNY PN  NGLNC GSH NEPGNKI++EDLTS PAYFS S+CKPRCL DGPV
Sbjct: 481  TMKFLTRVSNYDPNFGNGLNCMGSHCNEPGNKIDTEDLTSLPAYFSGSSCKPRCLADGPV 540

Query: 541  PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
            PSGNSDNVVRISGL SPDEDSTLYCSDEQ+SENHVE PNEM+KN  TCSLVE +K+EVPL
Sbjct: 541  PSGNSDNVVRISGLTSPDEDSTLYCSDEQTSENHVEKPNEMVKNVQTCSLVEEEKVEVPL 600

Query: 601  G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
              K +NNLEES NDCPNYTSD LSHSCASGVVQKSSQNEEGGLHFSASM KTE+KV  IH
Sbjct: 601  DDKGDNNLEESPNDCPNYTSDDLSHSCASGVVQKSSQNEEGGLHFSASMLKTENKVPPIH 660

Query: 661  SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
            SILKKKGRRKCKKISEIKP+LPPQIDIVSV P  KTE WDIDG CSQLDMIEDQKSHIAD
Sbjct: 661  SILKKKGRRKCKKISEIKPSLPPQIDIVSVTPVKKTELWDIDGNCSQLDMIEDQKSHIAD 720

Query: 721  TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
            TK VDSHEK+LSLSPISCHSERKGSK  KNFDS KGSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721  TKIVDSHEKSLSLSPISCHSERKGSKFKKNFDSLKGSKTRKKKLNECQIEDDDLLVSAII 780

Query: 781  RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
            RNKDVSSSAAGFSH+RKY KSRAKMNRK QKSSCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781  RNKDVSSSAAGFSHIRKYLKSRAKMNRKRQKSSCKLLLRSLGNGEKNYKDGKWYAVGART 840

Query: 841  VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
            VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCC+ +LSISEFKSHAGFKF
Sbjct: 841  VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCNVLLSISEFKSHAGFKF 900

Query: 901  NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
            NR C NLFL+SGRPFMLCQLQAWSTEYKTR+S+TRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901  NRPCLNLFLNSGRPFMLCQLQAWSTEYKTRRSRTRTVQVDEDDRNDDSCGICGDGGELIC 960

Query: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
            CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICG LVN+EE SSSS+ALKC QCEQKYHG
Sbjct: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGGLVNYEETSSSSNALKCSQCEQKYHG 1020

Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
            QCLKQ+DI+SGVESHIWFCS SCQKIY ALQ++LGL NQ ANG SW LLRCIH DQKILS
Sbjct: 1021 QCLKQKDIDSGVESHIWFCSWSCQKIYTALQTRLGLINQIANGLSWMLLRCIHNDQKILS 1080

Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
            T RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV
Sbjct: 1081 TPRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140

Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
            ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200

Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSG-QTTETTVGIH 1260
            AAIPSLVETWTEGFGFV VEN+EKQSLH+FNLMVFPGTVLLKKALYVSG Q TET     
Sbjct: 1201 AAIPSLVETWTEGFGFVPVENDEKQSLHRFNLMVFPGTVLLKKALYVSGGQNTET----R 1260

Query: 1261 SGVQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLV 1320
             GVQLDTD K+QCDS + CPRMEMKC  Y ELQE NGEK+ DDH+   AP IDSSTLQLV
Sbjct: 1261 EGVQLDTDTKRQCDSNDACPRMEMKCLNYLELQEHNGEKMMDDHKGISAP-IDSSTLQLV 1320

Query: 1321 ESNGLDISPGQKPVES------NCCTDIVGATTETCTQEAKKLLKVEVGVERDIQLSEGK 1380
            ESNG++ S  QKPVES      NCCTDIVG  TET T EAK+ LKVEVG+E DIQ+SEGK
Sbjct: 1321 ESNGMNTSSAQKPVESVLQSDGNCCTDIVGGKTETRTHEAKEPLKVEVGIECDIQVSEGK 1380

Query: 1381 SWDEGVHGATMTRFVEPVVLT 1393
            SWDEGVH A MTRFVEPVVLT
Sbjct: 1381 SWDEGVHAAAMTRFVEPVVLT 1394

BLAST of Cucsat.G8928.T5 vs. NCBI nr
Match: XP_022976399.1 (increased DNA methylation 1 isoform X1 [Cucurbita maxima] >XP_022976400.1 increased DNA methylation 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 2199 bits (5698), Expect = 0.0
Identity = 1122/1402 (80.03%), Postives = 1204/1402 (85.88%), Query Frame = 0

Query: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
            MDFQDDGFEGS NEEIIF+EVFFGNG+S SN+RCP  AFGYEHG CKINDASLCSSSE S
Sbjct: 1    MDFQDDGFEGSPNEEIIFKEVFFGNGASRSNERCPRDAFGYEHGLCKINDASLCSSSELS 60

Query: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
             VS + YSRN+KLDECYNATENIRT SA NS PCK I VEG+D NA  KR+K S D  SD
Sbjct: 61   TVSSHLYSRNIKLDECYNATENIRTSSAPNSFPCKWIPVEGNDENACVKRMKRSPDERSD 120

Query: 121  SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
            SVP LV + +SSD   EPVS +C PAE CD ESFTFHIVESSR+GIISSCY L+  VE D
Sbjct: 121  SVPELVMVMKSSDIRGEPVSTSCCPAEVCDSESFTFHIVESSRRGIISSCYLLKHFVERD 180

Query: 181  SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
            SN  +PDA K+TSLNLEG+ EP+MV+KVSASPVSQESSMTRLLVA+PS+  +E+F SPL 
Sbjct: 181  SNRGEPDASKRTSLNLEGNDEPSMVDKVSASPVSQESSMTRLLVASPSN--NEQFGSPLQ 240

Query: 241  LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
            L VGQMK  CPELD SL TDL RDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241  LVVGQMKLQCPELDTSLMTDLKRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300

Query: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
            SPQGR  REFSKAWR CGELLFADRCSFVK+V SKEWTGIHQFLFDLSDTLL +GKEMNQ
Sbjct: 301  SPQGRTIREFSKAWRVCGELLFADRCSFVKEVGSKEWTGIHQFLFDLSDTLLQVGKEMNQ 360

Query: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
            LG TTSLA+CWVILDPYVVVVFI RKIG LR+GDLVRATCS+G+NG++KTD FVTL NED
Sbjct: 361  LGGTTSLAHCWVILDPYVVVVFIYRKIGTLRKGDLVRATCSIGVNGNNKTDTFVTLTNED 420

Query: 421  NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
            +  C LSADK+ASP+ + SPSAKSALTEA LKDLD GNCAFDEQTCDT FSNYYGHTEDG
Sbjct: 421  SSICSLSADKSASPLREQSPSAKSALTEAVLKDLDGGNCAFDEQTCDTIFSNYYGHTEDG 480

Query: 481  TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
            T K  TRVSNY P+L N  NCTGSH NE G KI+SEDLT          CKPRCL D PV
Sbjct: 481  TMKLSTRVSNYVPSLANVPNCTGSHCNETGCKIDSEDLT----------CKPRCLADCPV 540

Query: 541  PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
            PSGNSDNVVRISG  SPDEDSTL+C DE SSEN VE PNE++KN LTCSL E KK+EVPL
Sbjct: 541  PSGNSDNVVRISGTTSPDEDSTLHCLDEHSSENQVEKPNELVKNVLTCSLGEEKKVEVPL 600

Query: 601  G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
              KAEN+LEESLND  NYTSD LSHSCASGVV+KS+QNEEGGLHFSAS FKTE+KVSAIH
Sbjct: 601  DDKAENSLEESLNDYSNYTSDDLSHSCASGVVEKSTQNEEGGLHFSASKFKTENKVSAIH 660

Query: 661  SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
            S LKKKGRRKCKKISEI PTLPPQI+IVS  PG KT+       C+QLDMIEDQKSHIAD
Sbjct: 661  STLKKKGRRKCKKISEINPTLPPQINIVSTTPGKKTD-------CTQLDMIEDQKSHIAD 720

Query: 721  TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
            TKNVDSHEK+  LSPISCHSERKGSK  K FDS +GSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721  TKNVDSHEKSSFLSPISCHSERKGSKFKKKFDSLRGSKTRKKKLNECQIEDDDLLVSAII 780

Query: 781  RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
            RNKDV+SSA GFSH+RKY KSRAKMN KSQK SCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781  RNKDVNSSAIGFSHIRKYLKSRAKMNCKSQKRSCKLLLRSLGNGEKNYKDGKWYAIGART 840

Query: 841  VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
            VLSWLLDAGVISSNDIIQYQSPKD SVVKYGRITGDGIICNCCS++L+ISEFK HAGFKF
Sbjct: 841  VLSWLLDAGVISSNDIIQYQSPKDDSVVKYGRITGDGIICNCCSELLTISEFKCHAGFKF 900

Query: 901  NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
            NR C NLFLDSGRPFMLCQLQAWSTEYKTR SKTRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901  NRPCLNLFLDSGRPFMLCQLQAWSTEYKTRTSKTRTVQVDEDDRNDDSCGICGDGGELIC 960

Query: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
            CDNCPSTFHHSCLSIQELPEGNWYCLNCTCR CG LVN+EE SSSSDALKC QCEQKYHG
Sbjct: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRTCGGLVNYEETSSSSDALKCSQCEQKYHG 1020

Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
            QCLKQ+DI+ GV SH+WFCS SCQKIYA LQS+LGL NQFANGFSW LLRCIH +QKILS
Sbjct: 1021 QCLKQKDIDPGVGSHLWFCSASCQKIYAGLQSRLGLINQFANGFSWMLLRCIHNEQKILS 1080

Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
            T+RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV
Sbjct: 1081 TSRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140

Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
            ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200

Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIHS 1260
            AAIPSLVETWTEGFGF+ VE++EK SLH+FNLMVFPGT+LLKKALYVSGQ TETT GI S
Sbjct: 1201 AAIPSLVETWTEGFGFIPVEDDEKHSLHRFNLMVFPGTLLLKKALYVSGQNTETTQGIRS 1260

Query: 1261 GVQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLVE 1320
            GVQLDTD+KQ+CDS + C R EMK   YQELQE NGEK  DD E +PAP   S+T     
Sbjct: 1261 GVQLDTDSKQECDSEKACSRTEMKRLMYQELQEQNGEKTMDDCEGNPAPMSPSTT----- 1320

Query: 1321 SNGLDISPGQKPVES-------NCCTDIVGATTETCTQEAKKLLKVEV-GVERDIQLSEG 1380
            +N +D   GQK ++S        CCTD VGA +ET   E  + L+VEV G ERD+QLSEG
Sbjct: 1321 TNEMDTCSGQKSIQSVQEQSDGKCCTDEVGAASETHIHEGNESLEVEVVGTERDVQLSEG 1377

Query: 1381 KSWDEGVHGATMTRFVEPVVLT 1393
            KSWD GV    MT FVEP V T
Sbjct: 1381 KSWD-GVRAVGMTGFVEPGVQT 1377

BLAST of Cucsat.G8928.T5 vs. NCBI nr
Match: XP_022976401.1 (increased DNA methylation 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 2189 bits (5671), Expect = 0.0
Identity = 1119/1402 (79.81%), Postives = 1201/1402 (85.66%), Query Frame = 0

Query: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
            MDFQDDGFEGS NEEIIF+EVFFGNG+S SN+RCP  AFGYEHG CKINDASLCSSSE S
Sbjct: 1    MDFQDDGFEGSPNEEIIFKEVFFGNGASRSNERCPRDAFGYEHGLCKINDASLCSSSELS 60

Query: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
             VS + YSRN+KLDECYNATENIRT SA NS PCK I VEG+D NA  KR+K S D  SD
Sbjct: 61   TVSSHLYSRNIKLDECYNATENIRTSSAPNSFPCKWIPVEGNDENACVKRMKRSPDERSD 120

Query: 121  SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
            SVP LV + +SSD   EPVS +C PAE CD ESFTFHIVESSR+GIISSCY L+  VE D
Sbjct: 121  SVPELVMVMKSSDIRGEPVSTSCCPAEVCDSESFTFHIVESSRRGIISSCYLLKHFVERD 180

Query: 181  SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
            SN  +PDA K+TSLNLEG+ EP+MV+KVSASPVSQESSMTRLLVA+PS+  +E+F SPL 
Sbjct: 181  SNRGEPDASKRTSLNLEGNDEPSMVDKVSASPVSQESSMTRLLVASPSN--NEQFGSPLQ 240

Query: 241  LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
            L VGQMK  CPELD SL TDL RDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241  LVVGQMKLQCPELDTSLMTDLKRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300

Query: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
            SPQGR  REFSKAWR CGELLFADRCSFVK+V SKEWTGIHQFLFDLSDTLL +GKEMNQ
Sbjct: 301  SPQGRTIREFSKAWRVCGELLFADRCSFVKEVGSKEWTGIHQFLFDLSDTLLQVGKEMNQ 360

Query: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
            LG TTSLA+CWVILDPYVVVVFI RKIG LR+GDLVRATCS+G+NG++KTD FVTL NED
Sbjct: 361  LGGTTSLAHCWVILDPYVVVVFIYRKIGTLRKGDLVRATCSIGVNGNNKTDTFVTLTNED 420

Query: 421  NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
            +  C LSADK+ASP+ + SPSAKSALTEA LKDLD GNCAFDEQTCDT FSNYYGHTEDG
Sbjct: 421  SSICSLSADKSASPLREQSPSAKSALTEAVLKDLDGGNCAFDEQTCDTIFSNYYGHTEDG 480

Query: 481  TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
            T K  TRVSNY P+L N  NCTGSH NE G KI+SEDLT          CKPRCL D PV
Sbjct: 481  TMKLSTRVSNYVPSLANVPNCTGSHCNETGCKIDSEDLT----------CKPRCLADCPV 540

Query: 541  PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
            PSGNSDNVVRISG  SPDEDSTL+C DE SSEN VE PNE++KN LTCSL E KK+EVPL
Sbjct: 541  PSGNSDNVVRISGTTSPDEDSTLHCLDEHSSENQVEKPNELVKNVLTCSLGEEKKVEVPL 600

Query: 601  G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
              KAEN+LEESLND  NYTSD LSHSCASGVV+KS+QNEEGGLHFSAS FKTE+KVSAIH
Sbjct: 601  DDKAENSLEESLNDYSNYTSDDLSHSCASGVVEKSTQNEEGGLHFSASKFKTENKVSAIH 660

Query: 661  SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
            S LKKKGRRKCKKISEI PTLPPQI+IVS  PG KT+       C+QLDMIEDQKSHIAD
Sbjct: 661  STLKKKGRRKCKKISEINPTLPPQINIVSTTPGKKTD-------CTQLDMIEDQKSHIAD 720

Query: 721  TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
            TKNVDSHEK+  LSPISCHSERKGSK  K FDS +GSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721  TKNVDSHEKSSFLSPISCHSERKGSKFKKKFDSLRGSKTRKKKLNECQIEDDDLLVSAII 780

Query: 781  RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
            RNKDV+SSA GFSH+RKY KSRAKMN KSQK SCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781  RNKDVNSSAIGFSHIRKYLKSRAKMNCKSQKRSCKLLLRSLGNGEKNYKDGKWYAIGART 840

Query: 841  VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
            VLSWLLDAGVISSNDIIQYQSPKD SVVKYGRITGDGIICNCCS++L+ISEFK HAGFKF
Sbjct: 841  VLSWLLDAGVISSNDIIQYQSPKDDSVVKYGRITGDGIICNCCSELLTISEFKCHAGFKF 900

Query: 901  NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
            NR C NLFLDSGRPFMLCQLQAWSTEYKTR SKTRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901  NRPCLNLFLDSGRPFMLCQLQAWSTEYKTRTSKTRTVQVDEDDRNDDSCGICGDGGELIC 960

Query: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
            CDNCPSTFHHSCLSIQELPEGNWYCLNCTCR CG LVN+EE SSSSDALKC QCEQKYHG
Sbjct: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRTCGGLVNYEETSSSSDALKCSQCEQKYHG 1020

Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
            QCLKQ+DI+ GV SH+WFCS SCQKIYA LQS+LGL NQFANGFSW LLRCIH +QKILS
Sbjct: 1021 QCLKQKDIDPGVGSHLWFCSASCQKIYAGLQSRLGLINQFANGFSWMLLRCIHNEQKILS 1080

Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
            T+RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV
Sbjct: 1081 TSRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140

Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
            ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200

Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIHS 1260
            AAIPSLVETWTEGFGF+ VE++EK SLH+FNLMVFPGT+LLKKALYVSGQ TETT     
Sbjct: 1201 AAIPSLVETWTEGFGFIPVEDDEKHSLHRFNLMVFPGTLLLKKALYVSGQNTETT----Q 1260

Query: 1261 GVQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLVE 1320
            GVQLDTD+KQ+CDS + C R EMK   YQELQE NGEK  DD E +PAP   S+T     
Sbjct: 1261 GVQLDTDSKQECDSEKACSRTEMKRLMYQELQEQNGEKTMDDCEGNPAPMSPSTT----- 1320

Query: 1321 SNGLDISPGQKPVES-------NCCTDIVGATTETCTQEAKKLLKVEV-GVERDIQLSEG 1380
            +N +D   GQK ++S        CCTD VGA +ET   E  + L+VEV G ERD+QLSEG
Sbjct: 1321 TNEMDTCSGQKSIQSVQEQSDGKCCTDEVGAASETHIHEGNESLEVEVVGTERDVQLSEG 1373

Query: 1381 KSWDEGVHGATMTRFVEPVVLT 1393
            KSWD GV    MT FVEP V T
Sbjct: 1381 KSWD-GVRAVGMTGFVEPGVQT 1373

BLAST of Cucsat.G8928.T5 vs. ExPASy TrEMBL
Match: A0A1S4E4E1 (increased DNA methylation 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501805 PE=4 SV=1)

HSP 1 Score: 2598 bits (6735), Expect = 0.0
Identity = 1299/1393 (93.25%), Postives = 1321/1393 (94.83%), Query Frame = 0

Query: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
            MDFQDDGFEGSANEEIIFRE+FFGNGSSHSNKRCPHKAF YEH PCKINDASLCSSSEPS
Sbjct: 1    MDFQDDGFEGSANEEIIFREIFFGNGSSHSNKRCPHKAFSYEHRPCKINDASLCSSSEPS 60

Query: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
             VS YSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTD ASD
Sbjct: 61   TVSSYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDEASD 120

Query: 121  SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
            SVPNLVKLKQSSDSIR PVSANC PAEECD ESFTFHIVESSRQGIISSCYRL+DL EMD
Sbjct: 121  SVPNLVKLKQSSDSIRVPVSANCYPAEECDSESFTFHIVESSRQGIISSCYRLKDLEEMD 180

Query: 181  SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
            SNL DPDAVK+TSLNLEG+ EPNMVNKVSASPVSQESSMTRLLVA+P D I+EKF SPLH
Sbjct: 181  SNLGDPDAVKRTSLNLEGNDEPNMVNKVSASPVSQESSMTRLLVASP-DTINEKFGSPLH 240

Query: 241  LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
            LEVGQMKSLCPEL ASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241  LEVGQMKSLCPELGASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300

Query: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
            SPQGRAFREFSKAWRFCGELLFADRCSFVKDV+SKEWTGIHQFLFDLSDTLL  GKEMNQ
Sbjct: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVDSKEWTGIHQFLFDLSDTLLQFGKEMNQ 360

Query: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
            LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGS KTD FVTL+NED
Sbjct: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSGKTDAFVTLVNED 420

Query: 421  NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
            N  C LSADKNASP+HDNSPSAKSALTEAPLKDLD GNCAFDEQTCDTS SNYYGHTEDG
Sbjct: 421  NSICNLSADKNASPLHDNSPSAKSALTEAPLKDLDGGNCAFDEQTCDTSLSNYYGHTEDG 480

Query: 481  TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
            TTKFPTRVSNY PNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFS STCKPRCL DGPV
Sbjct: 481  TTKFPTRVSNYDPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSGSTCKPRCLADGPV 540

Query: 541  PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
            PSGNSDNVVRISGL SPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL
Sbjct: 541  PSGNSDNVVRISGLTSPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600

Query: 601  GKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIHS 660
             KAENNLEESLNDC NYTSDGLSHSCASGVVQKSSQNEEGGL+FSASMFKTEDKVSAIHS
Sbjct: 601  SKAENNLEESLNDCANYTSDGLSHSCASGVVQKSSQNEEGGLNFSASMFKTEDKVSAIHS 660

Query: 661  ILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIADT 720
            ILKKKGRRKCKKISEIKP LPPQIDI              DG+CSQLDMIEDQKSHIADT
Sbjct: 661  ILKKKGRRKCKKISEIKPNLPPQIDI--------------DGSCSQLDMIEDQKSHIADT 720

Query: 721  KNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAIIR 780
            KNVDSHEKNLSLSPISCHSERK SKL KNFDS KGSKTRKKKLNECQIEDDDLLVSAIIR
Sbjct: 721  KNVDSHEKNLSLSPISCHSERKSSKLKKNFDSLKGSKTRKKKLNECQIEDDDLLVSAIIR 780

Query: 781  NKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGARTV 840
            NKDVSSSAAGFSHVRKY KSRAKMNRKSQKSSCKLLLRSLG+GEKNYKDGKWYALGARTV
Sbjct: 781  NKDVSSSAAGFSHVRKYLKSRAKMNRKSQKSSCKLLLRSLGNGEKNYKDGKWYALGARTV 840

Query: 841  LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKFN 900
            LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCC D+LSIS+FKSHAGFKFN
Sbjct: 841  LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCGDLLSISKFKSHAGFKFN 900

Query: 901  RACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELICC 960
            RAC NLFLDSGRPFMLCQLQAWSTEYKTRKS+TRTVEVDEDDRNDDSCGICGDGGELICC
Sbjct: 901  RACLNLFLDSGRPFMLCQLQAWSTEYKTRKSRTRTVEVDEDDRNDDSCGICGDGGELICC 960

Query: 961  DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHGQ 1020
            DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICG LVN+EEISSSSDALKCFQCEQKYHGQ
Sbjct: 961  DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGGLVNYEEISSSSDALKCFQCEQKYHGQ 1020

Query: 1021 CLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILST 1080
            CLKQRDINSGVESHIWFCS SCQKIY ALQS+LGLTNQFANGFSW LLRCIH DQKILST
Sbjct: 1021 CLKQRDINSGVESHIWFCSDSCQKIYTALQSRLGLTNQFANGFSWMLLRCIHNDQKILST 1080

Query: 1081 ARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140
             RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI
Sbjct: 1081 PRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140

Query: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200
            LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA
Sbjct: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200

Query: 1201 AIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIHSG 1260
            AIPSLVETWTEGFGF  VENEEKQSLH+FNLMVFPGTVLLKKALYVSGQTTETTVG    
Sbjct: 1201 AIPSLVETWTEGFGFAPVENEEKQSLHRFNLMVFPGTVLLKKALYVSGQTTETTVG---- 1260

Query: 1261 VQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLVES 1320
            VQLDT+ KQQC+S EPCPRMEMKCSKYQELQE NGEK KDDHEA+PAPP+DSSTLQLVES
Sbjct: 1261 VQLDTNTKQQCNSNEPCPRMEMKCSKYQELQEHNGEKTKDDHEANPAPPVDSSTLQLVES 1320

Query: 1321 NGLDISPGQKPVESNCCTDIVGATTETCTQEAKKLLKVEVGVERDIQLSEGKSWDEGVHG 1380
            NGLD SPGQK VESNCCTDIVGATTETC  EAKKLLKVEVGVE DIQLSEGKSWDEGVHG
Sbjct: 1321 NGLDTSPGQKFVESNCCTDIVGATTETCNHEAKKLLKVEVGVECDIQLSEGKSWDEGVHG 1374

Query: 1381 ATMTRFVEPVVLT 1393
            ATMTRFVEPVVLT
Sbjct: 1381 ATMTRFVEPVVLT 1374

BLAST of Cucsat.G8928.T5 vs. ExPASy TrEMBL
Match: A0A6J1IND9 (increased DNA methylation 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476816 PE=4 SV=1)

HSP 1 Score: 2199 bits (5698), Expect = 0.0
Identity = 1122/1402 (80.03%), Postives = 1204/1402 (85.88%), Query Frame = 0

Query: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
            MDFQDDGFEGS NEEIIF+EVFFGNG+S SN+RCP  AFGYEHG CKINDASLCSSSE S
Sbjct: 1    MDFQDDGFEGSPNEEIIFKEVFFGNGASRSNERCPRDAFGYEHGLCKINDASLCSSSELS 60

Query: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
             VS + YSRN+KLDECYNATENIRT SA NS PCK I VEG+D NA  KR+K S D  SD
Sbjct: 61   TVSSHLYSRNIKLDECYNATENIRTSSAPNSFPCKWIPVEGNDENACVKRMKRSPDERSD 120

Query: 121  SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
            SVP LV + +SSD   EPVS +C PAE CD ESFTFHIVESSR+GIISSCY L+  VE D
Sbjct: 121  SVPELVMVMKSSDIRGEPVSTSCCPAEVCDSESFTFHIVESSRRGIISSCYLLKHFVERD 180

Query: 181  SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
            SN  +PDA K+TSLNLEG+ EP+MV+KVSASPVSQESSMTRLLVA+PS+  +E+F SPL 
Sbjct: 181  SNRGEPDASKRTSLNLEGNDEPSMVDKVSASPVSQESSMTRLLVASPSN--NEQFGSPLQ 240

Query: 241  LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
            L VGQMK  CPELD SL TDL RDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241  LVVGQMKLQCPELDTSLMTDLKRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300

Query: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
            SPQGR  REFSKAWR CGELLFADRCSFVK+V SKEWTGIHQFLFDLSDTLL +GKEMNQ
Sbjct: 301  SPQGRTIREFSKAWRVCGELLFADRCSFVKEVGSKEWTGIHQFLFDLSDTLLQVGKEMNQ 360

Query: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
            LG TTSLA+CWVILDPYVVVVFI RKIG LR+GDLVRATCS+G+NG++KTD FVTL NED
Sbjct: 361  LGGTTSLAHCWVILDPYVVVVFIYRKIGTLRKGDLVRATCSIGVNGNNKTDTFVTLTNED 420

Query: 421  NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
            +  C LSADK+ASP+ + SPSAKSALTEA LKDLD GNCAFDEQTCDT FSNYYGHTEDG
Sbjct: 421  SSICSLSADKSASPLREQSPSAKSALTEAVLKDLDGGNCAFDEQTCDTIFSNYYGHTEDG 480

Query: 481  TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
            T K  TRVSNY P+L N  NCTGSH NE G KI+SEDLT          CKPRCL D PV
Sbjct: 481  TMKLSTRVSNYVPSLANVPNCTGSHCNETGCKIDSEDLT----------CKPRCLADCPV 540

Query: 541  PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
            PSGNSDNVVRISG  SPDEDSTL+C DE SSEN VE PNE++KN LTCSL E KK+EVPL
Sbjct: 541  PSGNSDNVVRISGTTSPDEDSTLHCLDEHSSENQVEKPNELVKNVLTCSLGEEKKVEVPL 600

Query: 601  G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
              KAEN+LEESLND  NYTSD LSHSCASGVV+KS+QNEEGGLHFSAS FKTE+KVSAIH
Sbjct: 601  DDKAENSLEESLNDYSNYTSDDLSHSCASGVVEKSTQNEEGGLHFSASKFKTENKVSAIH 660

Query: 661  SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
            S LKKKGRRKCKKISEI PTLPPQI+IVS  PG KT+       C+QLDMIEDQKSHIAD
Sbjct: 661  STLKKKGRRKCKKISEINPTLPPQINIVSTTPGKKTD-------CTQLDMIEDQKSHIAD 720

Query: 721  TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
            TKNVDSHEK+  LSPISCHSERKGSK  K FDS +GSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721  TKNVDSHEKSSFLSPISCHSERKGSKFKKKFDSLRGSKTRKKKLNECQIEDDDLLVSAII 780

Query: 781  RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
            RNKDV+SSA GFSH+RKY KSRAKMN KSQK SCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781  RNKDVNSSAIGFSHIRKYLKSRAKMNCKSQKRSCKLLLRSLGNGEKNYKDGKWYAIGART 840

Query: 841  VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
            VLSWLLDAGVISSNDIIQYQSPKD SVVKYGRITGDGIICNCCS++L+ISEFK HAGFKF
Sbjct: 841  VLSWLLDAGVISSNDIIQYQSPKDDSVVKYGRITGDGIICNCCSELLTISEFKCHAGFKF 900

Query: 901  NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
            NR C NLFLDSGRPFMLCQLQAWSTEYKTR SKTRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901  NRPCLNLFLDSGRPFMLCQLQAWSTEYKTRTSKTRTVQVDEDDRNDDSCGICGDGGELIC 960

Query: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
            CDNCPSTFHHSCLSIQELPEGNWYCLNCTCR CG LVN+EE SSSSDALKC QCEQKYHG
Sbjct: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRTCGGLVNYEETSSSSDALKCSQCEQKYHG 1020

Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
            QCLKQ+DI+ GV SH+WFCS SCQKIYA LQS+LGL NQFANGFSW LLRCIH +QKILS
Sbjct: 1021 QCLKQKDIDPGVGSHLWFCSASCQKIYAGLQSRLGLINQFANGFSWMLLRCIHNEQKILS 1080

Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
            T+RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV
Sbjct: 1081 TSRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140

Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
            ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200

Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIHS 1260
            AAIPSLVETWTEGFGF+ VE++EK SLH+FNLMVFPGT+LLKKALYVSGQ TETT GI S
Sbjct: 1201 AAIPSLVETWTEGFGFIPVEDDEKHSLHRFNLMVFPGTLLLKKALYVSGQNTETTQGIRS 1260

Query: 1261 GVQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLVE 1320
            GVQLDTD+KQ+CDS + C R EMK   YQELQE NGEK  DD E +PAP   S+T     
Sbjct: 1261 GVQLDTDSKQECDSEKACSRTEMKRLMYQELQEQNGEKTMDDCEGNPAPMSPSTT----- 1320

Query: 1321 SNGLDISPGQKPVES-------NCCTDIVGATTETCTQEAKKLLKVEV-GVERDIQLSEG 1380
            +N +D   GQK ++S        CCTD VGA +ET   E  + L+VEV G ERD+QLSEG
Sbjct: 1321 TNEMDTCSGQKSIQSVQEQSDGKCCTDEVGAASETHIHEGNESLEVEVVGTERDVQLSEG 1377

Query: 1381 KSWDEGVHGATMTRFVEPVVLT 1393
            KSWD GV    MT FVEP V T
Sbjct: 1381 KSWD-GVRAVGMTGFVEPGVQT 1377

BLAST of Cucsat.G8928.T5 vs. ExPASy TrEMBL
Match: A0A6J1IGS9 (increased DNA methylation 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476816 PE=4 SV=1)

HSP 1 Score: 2189 bits (5671), Expect = 0.0
Identity = 1119/1402 (79.81%), Postives = 1201/1402 (85.66%), Query Frame = 0

Query: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
            MDFQDDGFEGS NEEIIF+EVFFGNG+S SN+RCP  AFGYEHG CKINDASLCSSSE S
Sbjct: 1    MDFQDDGFEGSPNEEIIFKEVFFGNGASRSNERCPRDAFGYEHGLCKINDASLCSSSELS 60

Query: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
             VS + YSRN+KLDECYNATENIRT SA NS PCK I VEG+D NA  KR+K S D  SD
Sbjct: 61   TVSSHLYSRNIKLDECYNATENIRTSSAPNSFPCKWIPVEGNDENACVKRMKRSPDERSD 120

Query: 121  SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
            SVP LV + +SSD   EPVS +C PAE CD ESFTFHIVESSR+GIISSCY L+  VE D
Sbjct: 121  SVPELVMVMKSSDIRGEPVSTSCCPAEVCDSESFTFHIVESSRRGIISSCYLLKHFVERD 180

Query: 181  SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
            SN  +PDA K+TSLNLEG+ EP+MV+KVSASPVSQESSMTRLLVA+PS+  +E+F SPL 
Sbjct: 181  SNRGEPDASKRTSLNLEGNDEPSMVDKVSASPVSQESSMTRLLVASPSN--NEQFGSPLQ 240

Query: 241  LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
            L VGQMK  CPELD SL TDL RDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241  LVVGQMKLQCPELDTSLMTDLKRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300

Query: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
            SPQGR  REFSKAWR CGELLFADRCSFVK+V SKEWTGIHQFLFDLSDTLL +GKEMNQ
Sbjct: 301  SPQGRTIREFSKAWRVCGELLFADRCSFVKEVGSKEWTGIHQFLFDLSDTLLQVGKEMNQ 360

Query: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
            LG TTSLA+CWVILDPYVVVVFI RKIG LR+GDLVRATCS+G+NG++KTD FVTL NED
Sbjct: 361  LGGTTSLAHCWVILDPYVVVVFIYRKIGTLRKGDLVRATCSIGVNGNNKTDTFVTLTNED 420

Query: 421  NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
            +  C LSADK+ASP+ + SPSAKSALTEA LKDLD GNCAFDEQTCDT FSNYYGHTEDG
Sbjct: 421  SSICSLSADKSASPLREQSPSAKSALTEAVLKDLDGGNCAFDEQTCDTIFSNYYGHTEDG 480

Query: 481  TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
            T K  TRVSNY P+L N  NCTGSH NE G KI+SEDLT          CKPRCL D PV
Sbjct: 481  TMKLSTRVSNYVPSLANVPNCTGSHCNETGCKIDSEDLT----------CKPRCLADCPV 540

Query: 541  PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
            PSGNSDNVVRISG  SPDEDSTL+C DE SSEN VE PNE++KN LTCSL E KK+EVPL
Sbjct: 541  PSGNSDNVVRISGTTSPDEDSTLHCLDEHSSENQVEKPNELVKNVLTCSLGEEKKVEVPL 600

Query: 601  G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
              KAEN+LEESLND  NYTSD LSHSCASGVV+KS+QNEEGGLHFSAS FKTE+KVSAIH
Sbjct: 601  DDKAENSLEESLNDYSNYTSDDLSHSCASGVVEKSTQNEEGGLHFSASKFKTENKVSAIH 660

Query: 661  SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
            S LKKKGRRKCKKISEI PTLPPQI+IVS  PG KT+       C+QLDMIEDQKSHIAD
Sbjct: 661  STLKKKGRRKCKKISEINPTLPPQINIVSTTPGKKTD-------CTQLDMIEDQKSHIAD 720

Query: 721  TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
            TKNVDSHEK+  LSPISCHSERKGSK  K FDS +GSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721  TKNVDSHEKSSFLSPISCHSERKGSKFKKKFDSLRGSKTRKKKLNECQIEDDDLLVSAII 780

Query: 781  RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
            RNKDV+SSA GFSH+RKY KSRAKMN KSQK SCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781  RNKDVNSSAIGFSHIRKYLKSRAKMNCKSQKRSCKLLLRSLGNGEKNYKDGKWYAIGART 840

Query: 841  VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
            VLSWLLDAGVISSNDIIQYQSPKD SVVKYGRITGDGIICNCCS++L+ISEFK HAGFKF
Sbjct: 841  VLSWLLDAGVISSNDIIQYQSPKDDSVVKYGRITGDGIICNCCSELLTISEFKCHAGFKF 900

Query: 901  NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
            NR C NLFLDSGRPFMLCQLQAWSTEYKTR SKTRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901  NRPCLNLFLDSGRPFMLCQLQAWSTEYKTRTSKTRTVQVDEDDRNDDSCGICGDGGELIC 960

Query: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
            CDNCPSTFHHSCLSIQELPEGNWYCLNCTCR CG LVN+EE SSSSDALKC QCEQKYHG
Sbjct: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRTCGGLVNYEETSSSSDALKCSQCEQKYHG 1020

Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
            QCLKQ+DI+ GV SH+WFCS SCQKIYA LQS+LGL NQFANGFSW LLRCIH +QKILS
Sbjct: 1021 QCLKQKDIDPGVGSHLWFCSASCQKIYAGLQSRLGLINQFANGFSWMLLRCIHNEQKILS 1080

Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
            T+RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV
Sbjct: 1081 TSRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140

Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
            ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200

Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIHS 1260
            AAIPSLVETWTEGFGF+ VE++EK SLH+FNLMVFPGT+LLKKALYVSGQ TETT     
Sbjct: 1201 AAIPSLVETWTEGFGFIPVEDDEKHSLHRFNLMVFPGTLLLKKALYVSGQNTETT----Q 1260

Query: 1261 GVQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLVE 1320
            GVQLDTD+KQ+CDS + C R EMK   YQELQE NGEK  DD E +PAP   S+T     
Sbjct: 1261 GVQLDTDSKQECDSEKACSRTEMKRLMYQELQEQNGEKTMDDCEGNPAPMSPSTT----- 1320

Query: 1321 SNGLDISPGQKPVES-------NCCTDIVGATTETCTQEAKKLLKVEV-GVERDIQLSEG 1380
            +N +D   GQK ++S        CCTD VGA +ET   E  + L+VEV G ERD+QLSEG
Sbjct: 1321 TNEMDTCSGQKSIQSVQEQSDGKCCTDEVGAASETHIHEGNESLEVEVVGTERDVQLSEG 1373

Query: 1381 KSWDEGVHGATMTRFVEPVVLT 1393
            KSWD GV    MT FVEP V T
Sbjct: 1381 KSWD-GVRAVGMTGFVEPGVQT 1373

BLAST of Cucsat.G8928.T5 vs. ExPASy TrEMBL
Match: A0A6J1FC42 (LOW QUALITY PROTEIN: increased DNA methylation 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442744 PE=4 SV=1)

HSP 1 Score: 2164 bits (5608), Expect = 0.0
Identity = 1100/1403 (78.40%), Postives = 1190/1403 (84.82%), Query Frame = 0

Query: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
            MDFQDDGFEGS NE+IIF+E+FFGNG+S SN+RCP +AFGYEHG CKINDASLCSSSE S
Sbjct: 1    MDFQDDGFEGSPNEDIIFKEIFFGNGASRSNERCPREAFGYEHGLCKINDASLCSSSELS 60

Query: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
             VS + YSRN+KLDECYNATEN+RT SA+NS PCK   VEG+D NA  KR+K STD  SD
Sbjct: 61   TVSSHLYSRNIKLDECYNATENVRTSSAANSFPCKLTPVEGNDENACAKRMKRSTDERSD 120

Query: 121  SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
            SVP+LV + +SSD I EPVS +C PAE CD ESFTFHIVESSR+GIISSCY L+  VE D
Sbjct: 121  SVPDLVMVMKSSDIIGEPVSTSCCPAEVCDSESFTFHIVESSRRGIISSCYLLKHFVERD 180

Query: 181  SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
            SN  +PDA K+TSLNLEG+ EP+MV+KV ASPVSQESSMTRLLVA+PSD ++E+F SPL 
Sbjct: 181  SNRGEPDASKRTSLNLEGNDEPSMVDKVGASPVSQESSMTRLLVASPSDTLNEQFGSPLQ 240

Query: 241  LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
            L VGQMK  CPELD SL TDL RDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241  LVVGQMKLQCPELDTSLMTDLKRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300

Query: 301  SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
            SPQG+  REFSKAWR CGELLFADRCSFVK+V SKEWTGIHQFLFDLSDTLL + KEMNQ
Sbjct: 301  SPQGKTIREFSKAWRVCGELLFADRCSFVKEVGSKEWTGIHQFLFDLSDTLLQVRKEMNQ 360

Query: 361  LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
            LG TTSLA+CWVILDPYVVVVFI RKIG LR+GDLVRATCS+G+NG++KTD FVTL NED
Sbjct: 361  LGGTTSLAHCWVILDPYVVVVFIYRKIGTLRKGDLVRATCSIGVNGNNKTDTFVTLTNED 420

Query: 421  NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
            +  C LSADKNASP+ + SPSAKSALTE  LKD D GNCAFDEQ CDT FSNYY  TED 
Sbjct: 421  SSICSLSADKNASPLREQSPSAKSALTEVVLKDFDGGNCAFDEQACDTIFSNYYAQTEDA 480

Query: 481  TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
            T K  TRVSNY P L NG NCTGSH NEPG KI+SED+T          CKPRCL D PV
Sbjct: 481  TMKLSTRVSNYVPRLVNGPNCTGSHCNEPGCKIDSEDIT----------CKPRCLADCPV 540

Query: 541  PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
            PSGNSDNVVRISG  SPDEDSTL+C DE SSEN VE PNE++KN LTCSL E KK+EVPL
Sbjct: 541  PSGNSDNVVRISGTTSPDEDSTLHCLDEHSSENQVEKPNELVKNVLTCSLGEEKKVEVPL 600

Query: 601  G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
              KAEN+LEESLND  NYTSD LSHSCASGVV+KS+QNEEGGLHFSAS FKTE+KVSAIH
Sbjct: 601  DDKAENSLEESLNDYSNYTSDDLSHSCASGVVEKSTQNEEGGLHFSASKFKTENKVSAIH 660

Query: 661  SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
            S  KKKGRRKCKKISEI PTLP QIDIVS  PG KT+       CSQLDMIEDQK HIAD
Sbjct: 661  STSKKKGRRKCKKISEINPTLPSQIDIVSATPGKKTD-------CSQLDMIEDQKCHIAD 720

Query: 721  TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
            TKNVDSHEK+  LSPISCHSERKGSK  K FDS +GSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721  TKNVDSHEKSSFLSPISCHSERKGSKFKKKFDSLRGSKTRKKKLNECQIEDDDLLVSAII 780

Query: 781  RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
            RNKDV+SSA GFSH+RKY KSRA MN KSQK SCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781  RNKDVNSSAIGFSHIRKYLKSRANMNCKSQKRSCKLLLRSLGNGEKNYKDGKWYAIGART 840

Query: 841  VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
            VLSWLLDAGVISSNDIIQYQSPKD SVVKYGRITGDGI+CNCCS++L+ISEFK HAGFKF
Sbjct: 841  VLSWLLDAGVISSNDIIQYQSPKDDSVVKYGRITGDGIVCNCCSELLTISEFKHHAGFKF 900

Query: 901  NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
            NR C NLFLDSGRPFMLCQLQAWSTEYKTR SKTRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901  NRPCLNLFLDSGRPFMLCQLQAWSTEYKTRTSKTRTVQVDEDDRNDDSCGICGDGGELIC 960

Query: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
            CDNCPSTFHHSCLSIQELPEGNWYCLNCTCR CG LVN+EE SSSSDALKC QCEQKYHG
Sbjct: 961  CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRTCGGLVNYEETSSSSDALKCSQCEQKYHG 1020

Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
            QCLKQ+DIN GV SHIWFCS SCQ IYA LQS+LGL NQ ANGFSW LLRCIH +QKILS
Sbjct: 1021 QCLKQKDINPGVGSHIWFCSASCQTIYAGLQSRLGLINQCANGFSWMLLRCIHNEQKILS 1080

Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
            T+RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRL+FHGFYTV
Sbjct: 1081 TSRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLNFHGFYTV 1140

Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
            ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200

Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGIHS 1260
            AAIPSLVETWTEGFGF+ VE++EKQSLH+FNLMVFPGT+LLKKALYVSGQ TETT GI S
Sbjct: 1201 AAIPSLVETWTEGFGFIPVEDDEKQSLHRFNLMVFPGTLLLKKALYVSGQNTETTQGIRS 1260

Query: 1261 GVQLDTDAKQQCDSCEPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPIDSSTLQLVE 1320
                    KQ+CDS + C R EMK   ++ELQE +GEK  DD E +PAP   ++T     
Sbjct: 1261 --------KQECDSEKACSRTEMKRLTFEELQEHDGEKTMDDREGNPAPMSPATT----- 1320

Query: 1321 SNGLDISPGQKPVES--------NCCTDIVGATTETCTQEAKKLLKVEV-GVERDIQLSE 1380
            +NG+D   GQK ++S         CCTD VGA +ET   E  + L+VEV G ERD+QL+E
Sbjct: 1321 TNGMDTCSGQKAIQSVQEQEPDGKCCTDEVGAASETHIHEGNESLEVEVVGTERDVQLTE 1372

Query: 1381 GKSWDEGVHGATMTRFVEPVVLT 1393
            GKSWD GV    MT FVE  VLT
Sbjct: 1381 GKSWD-GVRAVGMTGFVEAGVLT 1372

BLAST of Cucsat.G8928.T5 vs. ExPASy TrEMBL
Match: A0A6J1CQ48 (LOW QUALITY PROTEIN: increased DNA methylation 1 OS=Momordica charantia OX=3673 GN=LOC111013131 PE=4 SV=1)

HSP 1 Score: 2093 bits (5423), Expect = 0.0
Identity = 1073/1387 (77.36%), Postives = 1184/1387 (85.36%), Query Frame = 0

Query: 1    MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
            MDFQDD FEGSANE IIF+EVFFGN SSH NK+CP KAF YEH  CKINDASLCSSS+ S
Sbjct: 1    MDFQDDDFEGSANEHIIFKEVFFGNSSSHFNKKCPCKAFSYEHESCKINDASLCSSSKFS 60

Query: 61   AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDA--A 120
             V  +SYSRN+K DECY+A EN R+GS  NSL CK  SVE  + NAS KRIK+STD    
Sbjct: 61   TVCSHSYSRNIKPDECYSAIENNRSGSVRNSLQCKCTSVEDKNENASVKRIKLSTDEDEP 120

Query: 121  SDSVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVE 180
            SDS+P+L K+  SS+ IREP SA C PAE+CD ESFTFHIVESS QGIISSCY L++LVE
Sbjct: 121  SDSIPDLGKVMNSSEIIREPASAGCCPAEDCDCESFTFHIVESSSQGIISSCYLLKNLVE 180

Query: 181  MDSNLADPDAVKQTSLNLEGHGEPNMV-NKVSASPVSQESSMTRLLVANPSDKISEKFRS 240
            MDSN+ DP   K T+LNLEG+ E NMV NKV AS VSQESSMTRLLVA+PS  + EKF S
Sbjct: 181  MDSNVGDPHVSKCTTLNLEGNDESNMVVNKVGASSVSQESSMTRLLVASPSVTLDEKFGS 240

Query: 241  PLHLEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMET 300
            PLHL+VGQ +  CPELD SLKTDL RDPRPLLHYHVVHLFIAAGWSIER KRPCRRY+ET
Sbjct: 241  PLHLDVGQTRFQCPELDTSLKTDLVRDPRPLLHYHVVHLFIAAGWSIERRKRPCRRYLET 300

Query: 301  VYRSPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKE 360
            VYRSPQGR  REFSKAWR CGELLFA+RCSFVK+V+SKEWTGIHQFLFDLSDTLL +GKE
Sbjct: 301  VYRSPQGRVIREFSKAWRICGELLFANRCSFVKEVDSKEWTGIHQFLFDLSDTLLQVGKE 360

Query: 361  MNQLGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLI 420
            MN LGATT LA+CWVILDPYVVVVFIDRKIG LR+GD+VRAT S+GINGS+KTD FVTL 
Sbjct: 361  MNNLGATTMLAHCWVILDPYVVVVFIDRKIGTLRKGDVVRATRSIGINGSNKTDTFVTLT 420

Query: 421  NEDNGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHT 480
            N D+  C   A+KN SP+HD+SPSAKSALTE  LKDLD G  AFDEQTCDTSFSNYYGHT
Sbjct: 421  NADS-MCNTFANKNTSPLHDHSPSAKSALTETVLKDLDGGYSAFDEQTCDTSFSNYYGHT 480

Query: 481  EDGTTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGD 540
            E+GT  F TRV +Y PN+  G +C GSH NE G KI+S+DL S PAY S STCK RCL D
Sbjct: 481  EEGTVNFSTRVCDYVPNVGTGPDCMGSHCNELGKKIDSKDLASLPAYLSGSTCKHRCLPD 540

Query: 541  GPVPSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLE 600
            G VPSGNSDNVVR+S   SPD+DSTLYCSDEQSSEN VE PNEM KN L  SL E  ++E
Sbjct: 541  G-VPSGNSDNVVRVSCPVSPDQDSTLYCSDEQSSENQVEKPNEMDKNVLMSSLGEELQVE 600

Query: 601  VPLG-KAENNLEESLNDCPNYT-SDGLSHSCASGVVQKSSQNEE-GGLHFSASMFKTEDK 660
            VPL  K ENNL+ESLNDC NYT SD LSHSCASGVVQKS+QNEE GGL F+AS  +TE+K
Sbjct: 601  VPLNDKVENNLDESLNDCQNYTTSDDLSHSCASGVVQKSTQNEEEGGLLFAASKLETENK 660

Query: 661  VSAIHSILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQK 720
            VSA HSILKKK RRKCK+ISEI PT+PPQIDIV+V PG KT+ WDIDG CSQLDMIEDQK
Sbjct: 661  VSAAHSILKKKWRRKCKRISEINPTVPPQIDIVNVTPGKKTKLWDIDGNCSQLDMIEDQK 720

Query: 721  SHIADTKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLL 780
            S IADTKN D HEK+LSLSP+SC+SERKGSK  K +DS +GSKTRKKKL ECQIEDDDLL
Sbjct: 721  SQIADTKNKDIHEKSLSLSPMSCYSERKGSKFKKIYDSLRGSKTRKKKLGECQIEDDDLL 780

Query: 781  VSAIIRNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYA 840
            VSAIIRNKD  SS AGFS VRK+ KSRAK +RKSQKSSCKLLLRSLG+GEK+YKDGKWY 
Sbjct: 781  VSAIIRNKDFGSSTAGFSPVRKFLKSRAKRDRKSQKSSCKLLLRSLGNGEKSYKDGKWYN 840

Query: 841  LGARTVLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSH 900
            +GARTVLSWLLDAGVISSNDIIQYQ+PKD SVVKYGRITGDGIICNCCS++L+ISEFKSH
Sbjct: 841  IGARTVLSWLLDAGVISSNDIIQYQNPKDDSVVKYGRITGDGIICNCCSELLTISEFKSH 900

Query: 901  AGFKFNRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDG 960
            +GFKF+R C NLFLDSG+PFMLCQLQAWSTEYKTR+S+T TV+VDEDDRNDDSCG+CGDG
Sbjct: 901  SGFKFSRPCLNLFLDSGKPFMLCQLQAWSTEYKTRRSRTGTVQVDEDDRNDDSCGVCGDG 960

Query: 961  GELICCDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCE 1020
            GELICCDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICG LV++EE SSSSDALKC QCE
Sbjct: 961  GELICCDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGGLVDYEEASSSSDALKCSQCE 1020

Query: 1021 QKYHGQCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYD 1080
            QKYHG+CLKQ+DI+ GVESHIWFCSGSCQK+YA LQS LGL NQF +G+SW LLRCIH D
Sbjct: 1021 QKYHGRCLKQKDIDPGVESHIWFCSGSCQKVYAGLQSLLGLINQFPDGYSWMLLRCIHND 1080

Query: 1081 QKILSTARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFH 1140
            QK+LST RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKS+FPRLDFH
Sbjct: 1081 QKVLSTQRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSNFPRLDFH 1140

Query: 1141 GFYTVILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKV 1200
            GFYTVILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKV
Sbjct: 1141 GFYTVILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKV 1200

Query: 1201 KKLVIAAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETT 1260
            KKLVIAAIP+LVETWTEGFGF+ VE++EK+SLH+FNLMVFPGT+LLKKALY SGQ TE T
Sbjct: 1201 KKLVIAAIPTLVETWTEGFGFIPVEDDEKKSLHRFNLMVFPGTILLKKALYSSGQNTEKT 1260

Query: 1261 VGIHSGVQLDTDAKQQCD--SC--EPCPRMEMKCSKYQELQEPNGEKIKDDHEADPAPPI 1320
             G  S  + D ++KQ CD  SC  + CPRMEM+CSKY+EL E N +K       +PAP I
Sbjct: 1261 EGTQSVEEFDAESKQHCDGHSCPDKACPRMEMQCSKYEELPEHNDQKT-----GNPAP-I 1320

Query: 1321 DSSTLQLVESNGLDISPGQKPVES------NCCTDIVGATTETCTQEAKKLLKVEVGVER 1371
            +SS+ QL ESNG+D +P QK V+S      NC TD VGATTET TQE K+LL+V+VG E 
Sbjct: 1321 NSSS-QLDESNGVDDTPEQKSVKSVIQSDGNCXTDKVGATTETSTQEGKELLEVDVGAEC 1378