Homology
BLAST of Cucsat.G8928.T22 vs. ExPASy Swiss-Prot
Match:
F4IXE7 (Increased DNA methylation 1 OS=Arabidopsis thaliana OX=3702 GN=IDM1 PE=1 SV=1)
HSP 1 Score: 697.2 bits (1798), Expect = 3.4e-199
Identity = 469/1260 (37.22%), Postives = 640/1260 (50.79%), Query Frame = 0
Query: 6 DGFEGSANEEIIFREVFFGNGSSHSNKRC-PHKAFGYEHGPCKINDASLCSSSEPSAVSI 65
D FEGS + IFREVFFG+ ++ KRC A +E K ++SL S+S V
Sbjct: 13 DCFEGSYEDHQIFREVFFGSDPGNTTKRCLVTGAINFECDSSKNVNSSLSSNS----VVT 72
Query: 66 YSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASDSVPN 125
Y+ C E SAS +G D N KR+K+S + D+
Sbjct: 73 SGYA-------CPQGFE----ASASR---------DGSDFNTKAKRVKLSGNKHLDA--- 132
Query: 126 LVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLV--EMDSN 185
+ S + + P + E+ H+VESS +G+ +S Y L+ + +
Sbjct: 133 ----RDEKGS-----ALHGFPTSDIARETIPLHLVESSNKGVSTSSYLLKHSIVKGREVY 192
Query: 186 LADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLHLE 245
L + K SLNL+ K ASPVSQES TR++ S SEK PL L
Sbjct: 193 LGGIVSGKCKSLNLDKCDGKEF--KAIASPVSQESFATRMISVGASTPHSEKACFPLQLN 252
Query: 246 VGQMKSLCPELDAS---LKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPC-RRYMETV 305
G K EL S LK D DPRPLL+ +V + AA W IE+ +R R++++T
Sbjct: 253 NGS-KVSPNELIMSKTCLKIDPKEDPRPLLYKYVCKVLTAARWKIEKRERSAGRKHVDTF 312
Query: 306 YRSPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEM 365
Y SP+GR FREF AW+ G +L ADR + D +K+WTGI+ F DLS TLL I + M
Sbjct: 313 YISPEGRKFREFGSAWKALGGILLADR--KLMDTGTKKWTGINDFWSDLSLTLLDIEENM 372
Query: 366 NQLGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLIN 425
L + A W L+P+VVVVFI +++G LR+G+
Sbjct: 373 KNLNLANTRALWWSALEPFVVVVFISKQVGSLRKGN------------------------ 432
Query: 426 EDNGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTE 485
K+ +N++P
Sbjct: 433 ------KVEVARNSNP-------------------------------------------- 492
Query: 486 DGTTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDG 545
+K++ ED CL
Sbjct: 493 --------------------------------DKLKKEDTI--------------CLN-- 552
Query: 546 PVPSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEV 605
+ SG ++V+ +S E S L + + E H
Sbjct: 553 -LISGCPESVLTVS------EGSHLVHDVDANQEIH------------------------ 612
Query: 606 PLGKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAI 665
++LE S L G + +E AS ED
Sbjct: 613 ------SDLEVQTKISSQKVSSRLERQSIIGKEISGTHEQEASKGIVASKLIAED---MH 672
Query: 666 HSILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIA 725
S+++K R+ KKIS+IKP Q D + N EF D E H+
Sbjct: 673 ESVMRKNLHRRSKKISDIKPASLDQHDSLDSNSLNSFEFQD----------KEMGNIHLV 732
Query: 726 DTKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAI 785
+ D +N ++ C+S++ K K++ +DDDL+ S I
Sbjct: 733 SKGSRDERLRNEKMNNSCCNSKKGRKKARKHY-----------------TQDDDLMGSTI 792
Query: 786 IRNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGAR 845
RNK S + S +K K +A+ +++ + C+LL RS + E ++ G W LG R
Sbjct: 793 TRNKGKFSRS---SQKKKTQKPKARTKKRNNRGGCRLLPRSSSNVENHFFQGNWSILGPR 852
Query: 846 TVLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFK 905
TVLSWL+ VIS +++IQ + P D +VVK G +T DG++C CC+ +S+SEFK+HAGF
Sbjct: 853 TVLSWLIATKVISRDEVIQLRDPDDDTVVKTGLVTKDGVVCTCCNKTVSLSEFKNHAGFN 912
Query: 906 FNRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELI 965
N C NLF+ SG+PF CQL+AWS EYK R++ R + +DD NDDSCG+CGDGGELI
Sbjct: 913 QNCPCLNLFMGSGKPFASCQLEAWSAEYKARRNGWRLEKASDDDPNDDSCGVCGDGGELI 972
Query: 966 CCDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYH 1025
CCDNCPSTFH +CLS+Q LPEG+WYC +CTC IC +LV+ + + S KC QC KYH
Sbjct: 973 CCDNCPSTFHQACLSMQVLPEGSWYCSSCTCWICSELVS--DNAERSQDFKCSQCAHKYH 1032
Query: 1026 GQCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKIL 1085
G CL+ + +FC +C+K+Y L S++G+ N A+G SW++L+C D +
Sbjct: 1033 GTCLQGISKRRKLFPETYFCGKNCEKVYNGLSSRVGIINPNADGLSWSILKCFQEDGMVH 1036
Query: 1086 STARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYT 1145
S RLA+ AECNS+L VAL+IMEE FLSMVDPRTGIDMIPH++Y+W S+F RLDF GFYT
Sbjct: 1093 SARRLALKAECNSKLAVALSIMEESFLSMVDPRTGIDMIPHVLYNWGSTFARLDFDGFYT 1036
Query: 1146 VILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLV 1205
V++EKDDV++ VASIRVHG +AEMPL+ATCSKYRRQGMCR L+ AIEEMLMS KV+KLV
Sbjct: 1153 VVVEKDDVMISVASIRVHGVTIAEMPLVATCSKYRRQGMCRILVAAIEEMLMSLKVEKLV 1036
Query: 1206 IAAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGKC 1259
+AA+PSLVETWTEGFGF +++EE+ +L + NLMVFPGT LLKK LY S + + T G C
Sbjct: 1213 VAALPSLVETWTEGFGFKPMDDEERDALKRINLMVFPGTTLLKKTLYESTKPS-TMKGVC 1036
BLAST of Cucsat.G8928.T22 vs. ExPASy Swiss-Prot
Match:
Q14839 (Chromodomain-helicase-DNA-binding protein 4 OS=Homo sapiens OX=9606 GN=CHD4 PE=1 SV=2)
HSP 1 Score: 70.1 bits (170), Expect = 2.1e-10
Identity = 27/53 (50.94%), Postives = 37/53 (69.81%), Query Frame = 0
Query: 939 DEDDRNDDSCGICGDGGELICCDNCPSTFHHSCLS--IQELPEGNWYCLNCTC 990
+EDD + + C +C DGGEL+CCD CPS++H CL+ + E+P G W C CTC
Sbjct: 443 EEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTC 495
BLAST of Cucsat.G8928.T22 vs. ExPASy Swiss-Prot
Match:
Q6PDQ2 (Chromodomain-helicase-DNA-binding protein 4 OS=Mus musculus OX=10090 GN=Chd4 PE=1 SV=1)
HSP 1 Score: 70.1 bits (170), Expect = 2.1e-10
Identity = 27/53 (50.94%), Postives = 37/53 (69.81%), Query Frame = 0
Query: 939 DEDDRNDDSCGICGDGGELICCDNCPSTFHHSCLS--IQELPEGNWYCLNCTC 990
+EDD + + C +C DGGEL+CCD CPS++H CL+ + E+P G W C CTC
Sbjct: 436 EEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTC 488
BLAST of Cucsat.G8928.T22 vs. ExPASy Swiss-Prot
Match:
O43918 (Autoimmune regulator OS=Homo sapiens OX=9606 GN=AIRE PE=1 SV=1)
HSP 1 Score: 69.3 bits (168), Expect = 3.5e-10
Identity = 26/47 (55.32%), Postives = 34/47 (72.34%), Query Frame = 0
Query: 943 RNDDSCGICGDGGELICCDNCPSTFHHSCLS--IQELPEGNWYCLNC 988
+N+D C +C DGGELICCD CP FH +CLS ++E+P G W C +C
Sbjct: 294 KNEDECAVCRDGGELICCDGCPRAFHLACLSPPLREIPSGTWRCSSC 340
BLAST of Cucsat.G8928.T22 vs. ExPASy Swiss-Prot
Match:
O97159 (Chromodomain-helicase-DNA-binding protein Mi-2 homolog OS=Drosophila melanogaster OX=7227 GN=Mi-2 PE=1 SV=2)
HSP 1 Score: 68.6 bits (166), Expect = 6.0e-10
Identity = 25/55 (45.45%), Postives = 39/55 (70.91%), Query Frame = 0
Query: 937 EVDEDDRNDDSCGICGDGGELICCDNCPSTFHHSCLS--IQELPEGNWYCLNCTC 990
E ++DD + + C +C DGGEL+CCD+CPS +H CL+ + +P+G+W C C+C
Sbjct: 429 EEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPRCSC 483
BLAST of Cucsat.G8928.T22 vs. NCBI nr
Match:
XP_004146095.1 (increased DNA methylation 1 [Cucumis sativus] >XP_031739517.1 increased DNA methylation 1 [Cucumis sativus] >XP_031739518.1 increased DNA methylation 1 [Cucumis sativus] >KAE8649828.1 hypothetical protein Csa_012099 [Cucumis sativus])
HSP 1 Score: 2528 bits (6553), Expect = 0.0
Identity = 1251/1256 (99.60%), Postives = 1252/1256 (99.68%), Query Frame = 0
Query: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS
Sbjct: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
Query: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTD ASD
Sbjct: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDEASD 120
Query: 121 SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD
Sbjct: 121 SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
Query: 181 SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH
Sbjct: 181 SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
Query: 241 LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241 LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
Query: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ
Sbjct: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
Query: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED
Sbjct: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
Query: 421 NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
NGF KLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG
Sbjct: 421 NGFRKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
Query: 481 TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV
Sbjct: 481 TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
Query: 541 PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKN LTCSLVEGKKLEVPL
Sbjct: 541 PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNVLTCSLVEGKKLEVPL 600
Query: 601 GKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIHS 660
GKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIHS
Sbjct: 601 GKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIHS 660
Query: 661 ILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIADT 720
ILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIADT
Sbjct: 661 ILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIADT 720
Query: 721 KNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAIIR 780
KNVDSHEKNLSLSPISCHSERKGSKL KNFDSHKGSKTRKKKLNECQIEDDDLLVSAIIR
Sbjct: 721 KNVDSHEKNLSLSPISCHSERKGSKLKKNFDSHKGSKTRKKKLNECQIEDDDLLVSAIIR 780
Query: 781 NKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGARTV 840
NKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGARTV
Sbjct: 781 NKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGARTV 840
Query: 841 LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKFN 900
LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKFN
Sbjct: 841 LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKFN 900
Query: 901 RACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELICC 960
RACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELICC
Sbjct: 901 RACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELICC 960
Query: 961 DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHGQ 1020
DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHGQ
Sbjct: 961 DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHGQ 1020
Query: 1021 CLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILST 1080
CLKQRDI+SGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILST
Sbjct: 1021 CLKQRDIDSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILST 1080
Query: 1081 ARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140
ARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI
Sbjct: 1081 ARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140
Query: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200
LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA
Sbjct: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200
Query: 1201 AIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVG 1256
AIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVG
Sbjct: 1201 AIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVG 1256
BLAST of Cucsat.G8928.T22 vs. NCBI nr
Match:
XP_016903085.1 (PREDICTED: increased DNA methylation 1 isoform X1 [Cucumis melo] >XP_016903087.1 PREDICTED: increased DNA methylation 1 isoform X2 [Cucumis melo])
HSP 1 Score: 2367 bits (6135), Expect = 0.0
Identity = 1179/1256 (93.87%), Postives = 1197/1256 (95.30%), Query Frame = 0
Query: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
MDFQDDGFEGSANEEIIFRE+FFGNGSSHSNKRCPHKAF YEH PCKINDASLCSSSEPS
Sbjct: 1 MDFQDDGFEGSANEEIIFREIFFGNGSSHSNKRCPHKAFSYEHRPCKINDASLCSSSEPS 60
Query: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
VS YSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTD ASD
Sbjct: 61 TVSSYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDEASD 120
Query: 121 SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
SVPNLVKLKQSSDSIR PVSANC PAEECD ESFTFHIVESSRQGIISSCYRL+DL EMD
Sbjct: 121 SVPNLVKLKQSSDSIRVPVSANCYPAEECDSESFTFHIVESSRQGIISSCYRLKDLEEMD 180
Query: 181 SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
SNL DPDAVK+TSLNLEG+ EPNMVNKVSASPVSQESSMTRLLVA+P D I+EKF SPLH
Sbjct: 181 SNLGDPDAVKRTSLNLEGNDEPNMVNKVSASPVSQESSMTRLLVASP-DTINEKFGSPLH 240
Query: 241 LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
LEVGQMKSLCPEL ASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241 LEVGQMKSLCPELGASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
Query: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
SPQGRAFREFSKAWRFCGELLFADRCSFVKDV+SKEWTGIHQFLFDLSDTLL GKEMNQ
Sbjct: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVDSKEWTGIHQFLFDLSDTLLQFGKEMNQ 360
Query: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGS KTD FVTL+NED
Sbjct: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSGKTDAFVTLVNED 420
Query: 421 NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
N C LSADKNASP+HDNSPSAKSALTEAPLKDLD GNCAFDEQTCDTS SNYYGHTEDG
Sbjct: 421 NSICNLSADKNASPLHDNSPSAKSALTEAPLKDLDGGNCAFDEQTCDTSLSNYYGHTEDG 480
Query: 481 TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
TTKFPTRVSNY PNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFS STCKPRCL DGPV
Sbjct: 481 TTKFPTRVSNYDPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSGSTCKPRCLADGPV 540
Query: 541 PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
PSGNSDNVVRISGL SPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL
Sbjct: 541 PSGNSDNVVRISGLTSPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
Query: 601 GKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIHS 660
KAENNLEESLNDC NYTSDGLSHSCASGVVQKSSQNEEGGL+FSASMFKTEDKVSAIHS
Sbjct: 601 SKAENNLEESLNDCANYTSDGLSHSCASGVVQKSSQNEEGGLNFSASMFKTEDKVSAIHS 660
Query: 661 ILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIADT 720
ILKKKGRRKCKKISEIKP LPPQIDI DG+CSQLDMIEDQKSHIADT
Sbjct: 661 ILKKKGRRKCKKISEIKPNLPPQIDI--------------DGSCSQLDMIEDQKSHIADT 720
Query: 721 KNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAIIR 780
KNVDSHEKNLSLSPISCHSERK SKL KNFDS KGSKTRKKKLNECQIEDDDLLVSAIIR
Sbjct: 721 KNVDSHEKNLSLSPISCHSERKSSKLKKNFDSLKGSKTRKKKLNECQIEDDDLLVSAIIR 780
Query: 781 NKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGARTV 840
NKDVSSSAAGFSHVRKY KSRAKMNRKSQKSSCKLLLRSLG+GEKNYKDGKWYALGARTV
Sbjct: 781 NKDVSSSAAGFSHVRKYLKSRAKMNRKSQKSSCKLLLRSLGNGEKNYKDGKWYALGARTV 840
Query: 841 LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKFN 900
LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCC D+LSIS+FKSHAGFKFN
Sbjct: 841 LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCGDLLSISKFKSHAGFKFN 900
Query: 901 RACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELICC 960
RAC NLFLDSGRPFMLCQLQAWSTEYKTRKS+TRTVEVDEDDRNDDSCGICGDGGELICC
Sbjct: 901 RACLNLFLDSGRPFMLCQLQAWSTEYKTRKSRTRTVEVDEDDRNDDSCGICGDGGELICC 960
Query: 961 DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHGQ 1020
DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICG LVN+EEISSSSDALKCFQCEQKYHGQ
Sbjct: 961 DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGGLVNYEEISSSSDALKCFQCEQKYHGQ 1020
Query: 1021 CLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILST 1080
CLKQRDINSGVESHIWFCS SCQKIY ALQS+LGLTNQFANGFSW LLRCIH DQKILST
Sbjct: 1021 CLKQRDINSGVESHIWFCSDSCQKIYTALQSRLGLTNQFANGFSWMLLRCIHNDQKILST 1080
Query: 1081 ARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140
RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI
Sbjct: 1081 PRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140
Query: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200
LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA
Sbjct: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200
Query: 1201 AIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVG 1256
AIPSLVETWTEGFGF VENEEKQSLH+FNLMVFPGTVLLKKALYVSGQTTETTVG
Sbjct: 1201 AIPSLVETWTEGFGFAPVENEEKQSLHRFNLMVFPGTVLLKKALYVSGQTTETTVG 1241
BLAST of Cucsat.G8928.T22 vs. NCBI nr
Match:
XP_038898710.1 (increased DNA methylation 1 [Benincasa hispida] >XP_038898711.1 increased DNA methylation 1 [Benincasa hispida] >XP_038898712.1 increased DNA methylation 1 [Benincasa hispida])
HSP 1 Score: 2218 bits (5748), Expect = 0.0
Identity = 1103/1258 (87.68%), Postives = 1159/1258 (92.13%), Query Frame = 0
Query: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
MDFQDDGFEGS NEEIIF+EVFFGNGSS SN RCP K F YEHGPCKINDASLCSSSE S
Sbjct: 1 MDFQDDGFEGSVNEEIIFKEVFFGNGSSRSNMRCPRKTFSYEHGPCKINDASLCSSSELS 60
Query: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
VS YSYSRN+KLDECYN TENI+T SA +SLPCK SVEGD+ NAS KRIK+STD ASD
Sbjct: 61 TVSSYSYSRNIKLDECYNPTENIKTSSAPDSLPCKWTSVEGDNVNASAKRIKLSTDEASD 120
Query: 121 SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
SVPNLVK+K+SSDS+R+PVS NC P E+CD ESFTFHIVESSRQGIISSCY L+ VE D
Sbjct: 121 SVPNLVKVKESSDSVRDPVSTNCCPVEDCDSESFTFHIVESSRQGIISSCYLLKHFVERD 180
Query: 181 SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
SNL DPDA K+TSLNLEG+ EPNMVNKVSASPVSQESSMTRLLVA+P D ++EKF SPLH
Sbjct: 181 SNLGDPDA-KRTSLNLEGNDEPNMVNKVSASPVSQESSMTRLLVASP-DTLNEKFGSPLH 240
Query: 241 LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
LEVGQMK CPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241 LEVGQMKFPCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
Query: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
SPQGRAFREFSKAWR CGELLFADRCSFVK+V+SKEWTGIHQFLFDLSDTLL +GKEMNQ
Sbjct: 301 SPQGRAFREFSKAWRACGELLFADRCSFVKEVDSKEWTGIHQFLFDLSDTLLKVGKEMNQ 360
Query: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
LGATTSLA CWVILDPYVVVVFI RKIG LRRGD VRATCS+G+NG++KT+ FVTL NED
Sbjct: 361 LGATTSLAQCWVILDPYVVVVFIGRKIGTLRRGDSVRATCSIGVNGNNKTEAFVTLTNED 420
Query: 421 NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
N C LSADKNASP+HDNSPSAKSALTEA LKDLD GNCAFDEQTCDTSFS+YYGHTEDG
Sbjct: 421 NSICNLSADKNASPLHDNSPSAKSALTEAALKDLDGGNCAFDEQTCDTSFSHYYGHTEDG 480
Query: 481 TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
T KF TRVSNY PN NGLNC GSH NEPGNKI++EDLTS PAYFS S+CKPRCL DGPV
Sbjct: 481 TMKFLTRVSNYDPNFGNGLNCMGSHCNEPGNKIDTEDLTSLPAYFSGSSCKPRCLADGPV 540
Query: 541 PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
PSGNSDNVVRISGL SPDEDSTLYCSDEQ+SENHVE PNEM+KN TCSLVE +K+EVPL
Sbjct: 541 PSGNSDNVVRISGLTSPDEDSTLYCSDEQTSENHVEKPNEMVKNVQTCSLVEEEKVEVPL 600
Query: 601 G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
K +NNLEES NDCPNYTSD LSHSCASGVVQKSSQNEEGGLHFSASM KTE+KV IH
Sbjct: 601 DDKGDNNLEESPNDCPNYTSDDLSHSCASGVVQKSSQNEEGGLHFSASMLKTENKVPPIH 660
Query: 661 SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
SILKKKGRRKCKKISEIKP+LPPQIDIVSV P KTE WDIDG CSQLDMIEDQKSHIAD
Sbjct: 661 SILKKKGRRKCKKISEIKPSLPPQIDIVSVTPVKKTELWDIDGNCSQLDMIEDQKSHIAD 720
Query: 721 TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
TK VDSHEK+LSLSPISCHSERKGSK KNFDS KGSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721 TKIVDSHEKSLSLSPISCHSERKGSKFKKNFDSLKGSKTRKKKLNECQIEDDDLLVSAII 780
Query: 781 RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
RNKDVSSSAAGFSH+RKY KSRAKMNRK QKSSCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781 RNKDVSSSAAGFSHIRKYLKSRAKMNRKRQKSSCKLLLRSLGNGEKNYKDGKWYAVGART 840
Query: 841 VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCC+ +LSISEFKSHAGFKF
Sbjct: 841 VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCNVLLSISEFKSHAGFKF 900
Query: 901 NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
NR C NLFL+SGRPFMLCQLQAWSTEYKTR+S+TRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901 NRPCLNLFLNSGRPFMLCQLQAWSTEYKTRRSRTRTVQVDEDDRNDDSCGICGDGGELIC 960
Query: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICG LVN+EE SSSS+ALKC QCEQKYHG
Sbjct: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGGLVNYEETSSSSNALKCSQCEQKYHG 1020
Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
QCLKQ+DI+SGVESHIWFCS SCQKIY ALQ++LGL NQ ANG SW LLRCIH DQKILS
Sbjct: 1021 QCLKQKDIDSGVESHIWFCSWSCQKIYTALQTRLGLINQIANGLSWMLLRCIHNDQKILS 1080
Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
T RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV
Sbjct: 1081 TPRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200
Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSG-QTTETTVG 1256
AAIPSLVETWTEGFGFV VEN+EKQSLH+FNLMVFPGTVLLKKALYVSG Q TET G
Sbjct: 1201 AAIPSLVETWTEGFGFVPVENDEKQSLHRFNLMVFPGTVLLKKALYVSGGQNTETREG 1256
BLAST of Cucsat.G8928.T22 vs. NCBI nr
Match:
KAG7024783.1 (Increased DNA methylation 1, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2080 bits (5389), Expect = 0.0
Identity = 1033/1265 (81.66%), Postives = 1115/1265 (88.14%), Query Frame = 0
Query: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
MDFQDDGFEGS NE+IIF+E+FFGNG+S SN+RCP +AFGYEHG CKINDASLCSSSE S
Sbjct: 1 MDFQDDGFEGSPNEDIIFKEIFFGNGASRSNERCPREAFGYEHGLCKINDASLCSSSELS 60
Query: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
VS + YSRN+KLDECYNATEN+RT SA+NS PCK VEG+D NA KR+K STD SD
Sbjct: 61 TVSSHLYSRNIKLDECYNATENVRTSSAANSFPCKLTPVEGNDENACAKRMKCSTDERSD 120
Query: 121 SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
SVP+LV + +SSD I EPVS +C PAE CD ESFTFHIVESSR+GIISSCY L+ VE D
Sbjct: 121 SVPDLVMVMKSSDIIGEPVSTSCCPAEVCDSESFTFHIVESSRRGIISSCYLLKHFVERD 180
Query: 181 SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
SN +PDA K+TSLNLEG+ EP+MV+KV ASPVSQESSMTRLLVA+PSD ++E+F SPL
Sbjct: 181 SNRGEPDASKRTSLNLEGNDEPSMVDKVGASPVSQESSMTRLLVASPSDTLNEQFGSPLQ 240
Query: 241 LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
L VGQMK CPELD SL DL RDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241 LVVGQMKLQCPELDTSLMADLKRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
Query: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
SPQGR REFSKAWR CGELLFADRCSFVK+V SKEWTGIHQFLFDLSDTLL +GKEM+Q
Sbjct: 301 SPQGRTIREFSKAWRVCGELLFADRCSFVKEVGSKEWTGIHQFLFDLSDTLLQVGKEMSQ 360
Query: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
LG TTSLA+CWVILDPYVVVVFI RKIG LR+GDLVRATCS+G+NG++KTD FVTL NED
Sbjct: 361 LGGTTSLAHCWVILDPYVVVVFIYRKIGTLRKGDLVRATCSIGVNGNNKTDTFVTLTNED 420
Query: 421 NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
+ C LSADKNASP+ + SPSAKSALTEA LKDLD GNCAFDEQ CDT FS+YY HTED
Sbjct: 421 SSICSLSADKNASPLREQSPSAKSALTEAVLKDLDGGNCAFDEQACDTIFSSYYAHTEDA 480
Query: 481 TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
T K TRVSNY P+L NG NCTGSH NEPG KI+SED+T CKPRCL D PV
Sbjct: 481 TMKLSTRVSNYVPSLANGPNCTGSHCNEPGCKIDSEDIT----------CKPRCLADCPV 540
Query: 541 PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
PSGNSDNVVRISG SPDEDSTL+C DE SSEN VE PNE+++N LTCSL E KK+EVPL
Sbjct: 541 PSGNSDNVVRISGATSPDEDSTLHCLDEHSSENQVEKPNELVRNVLTCSLGEEKKVEVPL 600
Query: 601 G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
KAEN+LEESLND NYTSD LSHSCASGVV+KS+QNEEGGLHFSAS FKTE+KVSA+H
Sbjct: 601 DDKAENSLEESLNDYSNYTSDDLSHSCASGVVEKSTQNEEGGLHFSASKFKTENKVSALH 660
Query: 661 SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
KKKGRRKCKKISEI PTLPPQID VS PG KT+ CSQLDM ED+KSHIAD
Sbjct: 661 PTSKKKGRRKCKKISEINPTLPPQIDSVSATPGKKTD-------CSQLDMREDRKSHIAD 720
Query: 721 TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
TKNVDSHEK+ LSPISCHSERKGSK K FDS +GSKTRKKKLNECQIEDDDLL+SAII
Sbjct: 721 TKNVDSHEKSSFLSPISCHSERKGSKFKKKFDSLRGSKTRKKKLNECQIEDDDLLISAII 780
Query: 781 RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
RNKDV+SSA GFSH+RKY KSRA MN KSQK SCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781 RNKDVNSSAIGFSHIRKYLKSRANMNCKSQKRSCKLLLRSLGNGEKNYKDGKWYAVGART 840
Query: 841 VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
VLSWLLDAGVISSNDIIQYQSPKD SVVKYGRITGDGI+CNCCS++L+ISEFK HAGFKF
Sbjct: 841 VLSWLLDAGVISSNDIIQYQSPKDDSVVKYGRITGDGIVCNCCSELLTISEFKHHAGFKF 900
Query: 901 NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
NR C NLFLDSGRPFMLCQLQAWSTEYKTR SKTRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901 NRPCLNLFLDSGRPFMLCQLQAWSTEYKTRTSKTRTVQVDEDDRNDDSCGICGDGGELIC 960
Query: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
CDNCPSTFHHSCLSIQELPEGNWYCLNCTCR CG LVN+EE SSSSDALKC QCEQKYHG
Sbjct: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRTCGGLVNYEETSSSSDALKCSQCEQKYHG 1020
Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
QCLKQ+DI+ GV SHIWFCS SCQ IYA LQS+LGL NQ ANGFSW LL+CIH +QKILS
Sbjct: 1021 QCLKQKDIDPGVGSHIWFCSASCQTIYAGLQSRLGLINQCANGFSWMLLKCIHNEQKILS 1080
Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
T+RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV
Sbjct: 1081 TSRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200
Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVGKCH 1260
AAIPSLVETWTEGFGF+ VE++EKQSLH+FNLMVFPGT+LLKKALYVSGQ TETT GKCH
Sbjct: 1201 AAIPSLVETWTEGFGFIPVEDDEKQSLHRFNLMVFPGTLLLKKALYVSGQNTETTQGKCH 1248
Query: 1261 LFPIM 1264
LFP++
Sbjct: 1261 LFPVL 1248
BLAST of Cucsat.G8928.T22 vs. NCBI nr
Match:
XP_022976401.1 (increased DNA methylation 1 isoform X2 [Cucurbita maxima])
HSP 1 Score: 2073 bits (5370), Expect = 0.0
Identity = 1042/1257 (82.90%), Postives = 1111/1257 (88.39%), Query Frame = 0
Query: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
MDFQDDGFEGS NEEIIF+EVFFGNG+S SN+RCP AFGYEHG CKINDASLCSSSE S
Sbjct: 1 MDFQDDGFEGSPNEEIIFKEVFFGNGASRSNERCPRDAFGYEHGLCKINDASLCSSSELS 60
Query: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
VS + YSRN+KLDECYNATENIRT SA NS PCK I VEG+D NA KR+K S D SD
Sbjct: 61 TVSSHLYSRNIKLDECYNATENIRTSSAPNSFPCKWIPVEGNDENACVKRMKRSPDERSD 120
Query: 121 SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
SVP LV + +SSD EPVS +C PAE CD ESFTFHIVESSR+GIISSCY L+ VE D
Sbjct: 121 SVPELVMVMKSSDIRGEPVSTSCCPAEVCDSESFTFHIVESSRRGIISSCYLLKHFVERD 180
Query: 181 SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
SN +PDA K+TSLNLEG+ EP+MV+KVSASPVSQESSMTRLLVA+PS+ +E+F SPL
Sbjct: 181 SNRGEPDASKRTSLNLEGNDEPSMVDKVSASPVSQESSMTRLLVASPSN--NEQFGSPLQ 240
Query: 241 LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
L VGQMK CPELD SL TDL RDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241 LVVGQMKLQCPELDTSLMTDLKRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
Query: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
SPQGR REFSKAWR CGELLFADRCSFVK+V SKEWTGIHQFLFDLSDTLL +GKEMNQ
Sbjct: 301 SPQGRTIREFSKAWRVCGELLFADRCSFVKEVGSKEWTGIHQFLFDLSDTLLQVGKEMNQ 360
Query: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
LG TTSLA+CWVILDPYVVVVFI RKIG LR+GDLVRATCS+G+NG++KTD FVTL NED
Sbjct: 361 LGGTTSLAHCWVILDPYVVVVFIYRKIGTLRKGDLVRATCSIGVNGNNKTDTFVTLTNED 420
Query: 421 NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
+ C LSADK+ASP+ + SPSAKSALTEA LKDLD GNCAFDEQTCDT FSNYYGHTEDG
Sbjct: 421 SSICSLSADKSASPLREQSPSAKSALTEAVLKDLDGGNCAFDEQTCDTIFSNYYGHTEDG 480
Query: 481 TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
T K TRVSNY P+L N NCTGSH NE G KI+SEDLT CKPRCL D PV
Sbjct: 481 TMKLSTRVSNYVPSLANVPNCTGSHCNETGCKIDSEDLT----------CKPRCLADCPV 540
Query: 541 PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
PSGNSDNVVRISG SPDEDSTL+C DE SSEN VE PNE++KN LTCSL E KK+EVPL
Sbjct: 541 PSGNSDNVVRISGTTSPDEDSTLHCLDEHSSENQVEKPNELVKNVLTCSLGEEKKVEVPL 600
Query: 601 G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
KAEN+LEESLND NYTSD LSHSCASGVV+KS+QNEEGGLHFSAS FKTE+KVSAIH
Sbjct: 601 DDKAENSLEESLNDYSNYTSDDLSHSCASGVVEKSTQNEEGGLHFSASKFKTENKVSAIH 660
Query: 661 SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
S LKKKGRRKCKKISEI PTLPPQI+IVS PG KT+ C+QLDMIEDQKSHIAD
Sbjct: 661 STLKKKGRRKCKKISEINPTLPPQINIVSTTPGKKTD-------CTQLDMIEDQKSHIAD 720
Query: 721 TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
TKNVDSHEK+ LSPISCHSERKGSK K FDS +GSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721 TKNVDSHEKSSFLSPISCHSERKGSKFKKKFDSLRGSKTRKKKLNECQIEDDDLLVSAII 780
Query: 781 RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
RNKDV+SSA GFSH+RKY KSRAKMN KSQK SCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781 RNKDVNSSAIGFSHIRKYLKSRAKMNCKSQKRSCKLLLRSLGNGEKNYKDGKWYAIGART 840
Query: 841 VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
VLSWLLDAGVISSNDIIQYQSPKD SVVKYGRITGDGIICNCCS++L+ISEFK HAGFKF
Sbjct: 841 VLSWLLDAGVISSNDIIQYQSPKDDSVVKYGRITGDGIICNCCSELLTISEFKCHAGFKF 900
Query: 901 NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
NR C NLFLDSGRPFMLCQLQAWSTEYKTR SKTRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901 NRPCLNLFLDSGRPFMLCQLQAWSTEYKTRTSKTRTVQVDEDDRNDDSCGICGDGGELIC 960
Query: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
CDNCPSTFHHSCLSIQELPEGNWYCLNCTCR CG LVN+EE SSSSDALKC QCEQKYHG
Sbjct: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRTCGGLVNYEETSSSSDALKCSQCEQKYHG 1020
Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
QCLKQ+DI+ GV SH+WFCS SCQKIYA LQS+LGL NQFANGFSW LLRCIH +QKILS
Sbjct: 1021 QCLKQKDIDPGVGSHLWFCSASCQKIYAGLQSRLGLINQFANGFSWMLLRCIHNEQKILS 1080
Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
T+RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV
Sbjct: 1081 TSRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200
Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVG 1256
AAIPSLVETWTEGFGF+ VE++EK SLH+FNLMVFPGT+LLKKALYVSGQ TETT G
Sbjct: 1201 AAIPSLVETWTEGFGFIPVEDDEKHSLHRFNLMVFPGTLLLKKALYVSGQNTETTQG 1238
BLAST of Cucsat.G8928.T22 vs. ExPASy TrEMBL
Match:
A0A1S4E4E1 (increased DNA methylation 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501805 PE=4 SV=1)
HSP 1 Score: 2367 bits (6135), Expect = 0.0
Identity = 1179/1256 (93.87%), Postives = 1197/1256 (95.30%), Query Frame = 0
Query: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
MDFQDDGFEGSANEEIIFRE+FFGNGSSHSNKRCPHKAF YEH PCKINDASLCSSSEPS
Sbjct: 1 MDFQDDGFEGSANEEIIFREIFFGNGSSHSNKRCPHKAFSYEHRPCKINDASLCSSSEPS 60
Query: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
VS YSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTD ASD
Sbjct: 61 TVSSYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDEASD 120
Query: 121 SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
SVPNLVKLKQSSDSIR PVSANC PAEECD ESFTFHIVESSRQGIISSCYRL+DL EMD
Sbjct: 121 SVPNLVKLKQSSDSIRVPVSANCYPAEECDSESFTFHIVESSRQGIISSCYRLKDLEEMD 180
Query: 181 SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
SNL DPDAVK+TSLNLEG+ EPNMVNKVSASPVSQESSMTRLLVA+P D I+EKF SPLH
Sbjct: 181 SNLGDPDAVKRTSLNLEGNDEPNMVNKVSASPVSQESSMTRLLVASP-DTINEKFGSPLH 240
Query: 241 LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
LEVGQMKSLCPEL ASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241 LEVGQMKSLCPELGASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
Query: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
SPQGRAFREFSKAWRFCGELLFADRCSFVKDV+SKEWTGIHQFLFDLSDTLL GKEMNQ
Sbjct: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVDSKEWTGIHQFLFDLSDTLLQFGKEMNQ 360
Query: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGS KTD FVTL+NED
Sbjct: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSGKTDAFVTLVNED 420
Query: 421 NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
N C LSADKNASP+HDNSPSAKSALTEAPLKDLD GNCAFDEQTCDTS SNYYGHTEDG
Sbjct: 421 NSICNLSADKNASPLHDNSPSAKSALTEAPLKDLDGGNCAFDEQTCDTSLSNYYGHTEDG 480
Query: 481 TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
TTKFPTRVSNY PNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFS STCKPRCL DGPV
Sbjct: 481 TTKFPTRVSNYDPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSGSTCKPRCLADGPV 540
Query: 541 PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
PSGNSDNVVRISGL SPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL
Sbjct: 541 PSGNSDNVVRISGLTSPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
Query: 601 GKAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIHS 660
KAENNLEESLNDC NYTSDGLSHSCASGVVQKSSQNEEGGL+FSASMFKTEDKVSAIHS
Sbjct: 601 SKAENNLEESLNDCANYTSDGLSHSCASGVVQKSSQNEEGGLNFSASMFKTEDKVSAIHS 660
Query: 661 ILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIADT 720
ILKKKGRRKCKKISEIKP LPPQIDI DG+CSQLDMIEDQKSHIADT
Sbjct: 661 ILKKKGRRKCKKISEIKPNLPPQIDI--------------DGSCSQLDMIEDQKSHIADT 720
Query: 721 KNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAIIR 780
KNVDSHEKNLSLSPISCHSERK SKL KNFDS KGSKTRKKKLNECQIEDDDLLVSAIIR
Sbjct: 721 KNVDSHEKNLSLSPISCHSERKSSKLKKNFDSLKGSKTRKKKLNECQIEDDDLLVSAIIR 780
Query: 781 NKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGARTV 840
NKDVSSSAAGFSHVRKY KSRAKMNRKSQKSSCKLLLRSLG+GEKNYKDGKWYALGARTV
Sbjct: 781 NKDVSSSAAGFSHVRKYLKSRAKMNRKSQKSSCKLLLRSLGNGEKNYKDGKWYALGARTV 840
Query: 841 LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKFN 900
LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCC D+LSIS+FKSHAGFKFN
Sbjct: 841 LSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCGDLLSISKFKSHAGFKFN 900
Query: 901 RACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELICC 960
RAC NLFLDSGRPFMLCQLQAWSTEYKTRKS+TRTVEVDEDDRNDDSCGICGDGGELICC
Sbjct: 901 RACLNLFLDSGRPFMLCQLQAWSTEYKTRKSRTRTVEVDEDDRNDDSCGICGDGGELICC 960
Query: 961 DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHGQ 1020
DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICG LVN+EEISSSSDALKCFQCEQKYHGQ
Sbjct: 961 DNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGGLVNYEEISSSSDALKCFQCEQKYHGQ 1020
Query: 1021 CLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILST 1080
CLKQRDINSGVESHIWFCS SCQKIY ALQS+LGLTNQFANGFSW LLRCIH DQKILST
Sbjct: 1021 CLKQRDINSGVESHIWFCSDSCQKIYTALQSRLGLTNQFANGFSWMLLRCIHNDQKILST 1080
Query: 1081 ARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140
RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI
Sbjct: 1081 PRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTVI 1140
Query: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200
LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA
Sbjct: 1141 LEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVIA 1200
Query: 1201 AIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVG 1256
AIPSLVETWTEGFGF VENEEKQSLH+FNLMVFPGTVLLKKALYVSGQTTETTVG
Sbjct: 1201 AIPSLVETWTEGFGFAPVENEEKQSLHRFNLMVFPGTVLLKKALYVSGQTTETTVG 1241
BLAST of Cucsat.G8928.T22 vs. ExPASy TrEMBL
Match:
A0A6J1IND9 (increased DNA methylation 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476816 PE=4 SV=1)
HSP 1 Score: 2073 bits (5370), Expect = 0.0
Identity = 1042/1257 (82.90%), Postives = 1111/1257 (88.39%), Query Frame = 0
Query: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
MDFQDDGFEGS NEEIIF+EVFFGNG+S SN+RCP AFGYEHG CKINDASLCSSSE S
Sbjct: 1 MDFQDDGFEGSPNEEIIFKEVFFGNGASRSNERCPRDAFGYEHGLCKINDASLCSSSELS 60
Query: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
VS + YSRN+KLDECYNATENIRT SA NS PCK I VEG+D NA KR+K S D SD
Sbjct: 61 TVSSHLYSRNIKLDECYNATENIRTSSAPNSFPCKWIPVEGNDENACVKRMKRSPDERSD 120
Query: 121 SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
SVP LV + +SSD EPVS +C PAE CD ESFTFHIVESSR+GIISSCY L+ VE D
Sbjct: 121 SVPELVMVMKSSDIRGEPVSTSCCPAEVCDSESFTFHIVESSRRGIISSCYLLKHFVERD 180
Query: 181 SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
SN +PDA K+TSLNLEG+ EP+MV+KVSASPVSQESSMTRLLVA+PS+ +E+F SPL
Sbjct: 181 SNRGEPDASKRTSLNLEGNDEPSMVDKVSASPVSQESSMTRLLVASPSN--NEQFGSPLQ 240
Query: 241 LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
L VGQMK CPELD SL TDL RDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241 LVVGQMKLQCPELDTSLMTDLKRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
Query: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
SPQGR REFSKAWR CGELLFADRCSFVK+V SKEWTGIHQFLFDLSDTLL +GKEMNQ
Sbjct: 301 SPQGRTIREFSKAWRVCGELLFADRCSFVKEVGSKEWTGIHQFLFDLSDTLLQVGKEMNQ 360
Query: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
LG TTSLA+CWVILDPYVVVVFI RKIG LR+GDLVRATCS+G+NG++KTD FVTL NED
Sbjct: 361 LGGTTSLAHCWVILDPYVVVVFIYRKIGTLRKGDLVRATCSIGVNGNNKTDTFVTLTNED 420
Query: 421 NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
+ C LSADK+ASP+ + SPSAKSALTEA LKDLD GNCAFDEQTCDT FSNYYGHTEDG
Sbjct: 421 SSICSLSADKSASPLREQSPSAKSALTEAVLKDLDGGNCAFDEQTCDTIFSNYYGHTEDG 480
Query: 481 TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
T K TRVSNY P+L N NCTGSH NE G KI+SEDLT CKPRCL D PV
Sbjct: 481 TMKLSTRVSNYVPSLANVPNCTGSHCNETGCKIDSEDLT----------CKPRCLADCPV 540
Query: 541 PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
PSGNSDNVVRISG SPDEDSTL+C DE SSEN VE PNE++KN LTCSL E KK+EVPL
Sbjct: 541 PSGNSDNVVRISGTTSPDEDSTLHCLDEHSSENQVEKPNELVKNVLTCSLGEEKKVEVPL 600
Query: 601 G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
KAEN+LEESLND NYTSD LSHSCASGVV+KS+QNEEGGLHFSAS FKTE+KVSAIH
Sbjct: 601 DDKAENSLEESLNDYSNYTSDDLSHSCASGVVEKSTQNEEGGLHFSASKFKTENKVSAIH 660
Query: 661 SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
S LKKKGRRKCKKISEI PTLPPQI+IVS PG KT+ C+QLDMIEDQKSHIAD
Sbjct: 661 STLKKKGRRKCKKISEINPTLPPQINIVSTTPGKKTD-------CTQLDMIEDQKSHIAD 720
Query: 721 TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
TKNVDSHEK+ LSPISCHSERKGSK K FDS +GSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721 TKNVDSHEKSSFLSPISCHSERKGSKFKKKFDSLRGSKTRKKKLNECQIEDDDLLVSAII 780
Query: 781 RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
RNKDV+SSA GFSH+RKY KSRAKMN KSQK SCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781 RNKDVNSSAIGFSHIRKYLKSRAKMNCKSQKRSCKLLLRSLGNGEKNYKDGKWYAIGART 840
Query: 841 VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
VLSWLLDAGVISSNDIIQYQSPKD SVVKYGRITGDGIICNCCS++L+ISEFK HAGFKF
Sbjct: 841 VLSWLLDAGVISSNDIIQYQSPKDDSVVKYGRITGDGIICNCCSELLTISEFKCHAGFKF 900
Query: 901 NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
NR C NLFLDSGRPFMLCQLQAWSTEYKTR SKTRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901 NRPCLNLFLDSGRPFMLCQLQAWSTEYKTRTSKTRTVQVDEDDRNDDSCGICGDGGELIC 960
Query: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
CDNCPSTFHHSCLSIQELPEGNWYCLNCTCR CG LVN+EE SSSSDALKC QCEQKYHG
Sbjct: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRTCGGLVNYEETSSSSDALKCSQCEQKYHG 1020
Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
QCLKQ+DI+ GV SH+WFCS SCQKIYA LQS+LGL NQFANGFSW LLRCIH +QKILS
Sbjct: 1021 QCLKQKDIDPGVGSHLWFCSASCQKIYAGLQSRLGLINQFANGFSWMLLRCIHNEQKILS 1080
Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
T+RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV
Sbjct: 1081 TSRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200
Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVG 1256
AAIPSLVETWTEGFGF+ VE++EK SLH+FNLMVFPGT+LLKKALYVSGQ TETT G
Sbjct: 1201 AAIPSLVETWTEGFGFIPVEDDEKHSLHRFNLMVFPGTLLLKKALYVSGQNTETTQG 1238
BLAST of Cucsat.G8928.T22 vs. ExPASy TrEMBL
Match:
A0A6J1IGS9 (increased DNA methylation 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476816 PE=4 SV=1)
HSP 1 Score: 2073 bits (5370), Expect = 0.0
Identity = 1042/1257 (82.90%), Postives = 1111/1257 (88.39%), Query Frame = 0
Query: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
MDFQDDGFEGS NEEIIF+EVFFGNG+S SN+RCP AFGYEHG CKINDASLCSSSE S
Sbjct: 1 MDFQDDGFEGSPNEEIIFKEVFFGNGASRSNERCPRDAFGYEHGLCKINDASLCSSSELS 60
Query: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
VS + YSRN+KLDECYNATENIRT SA NS PCK I VEG+D NA KR+K S D SD
Sbjct: 61 TVSSHLYSRNIKLDECYNATENIRTSSAPNSFPCKWIPVEGNDENACVKRMKRSPDERSD 120
Query: 121 SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
SVP LV + +SSD EPVS +C PAE CD ESFTFHIVESSR+GIISSCY L+ VE D
Sbjct: 121 SVPELVMVMKSSDIRGEPVSTSCCPAEVCDSESFTFHIVESSRRGIISSCYLLKHFVERD 180
Query: 181 SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
SN +PDA K+TSLNLEG+ EP+MV+KVSASPVSQESSMTRLLVA+PS+ +E+F SPL
Sbjct: 181 SNRGEPDASKRTSLNLEGNDEPSMVDKVSASPVSQESSMTRLLVASPSN--NEQFGSPLQ 240
Query: 241 LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
L VGQMK CPELD SL TDL RDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241 LVVGQMKLQCPELDTSLMTDLKRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
Query: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
SPQGR REFSKAWR CGELLFADRCSFVK+V SKEWTGIHQFLFDLSDTLL +GKEMNQ
Sbjct: 301 SPQGRTIREFSKAWRVCGELLFADRCSFVKEVGSKEWTGIHQFLFDLSDTLLQVGKEMNQ 360
Query: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
LG TTSLA+CWVILDPYVVVVFI RKIG LR+GDLVRATCS+G+NG++KTD FVTL NED
Sbjct: 361 LGGTTSLAHCWVILDPYVVVVFIYRKIGTLRKGDLVRATCSIGVNGNNKTDTFVTLTNED 420
Query: 421 NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
+ C LSADK+ASP+ + SPSAKSALTEA LKDLD GNCAFDEQTCDT FSNYYGHTEDG
Sbjct: 421 SSICSLSADKSASPLREQSPSAKSALTEAVLKDLDGGNCAFDEQTCDTIFSNYYGHTEDG 480
Query: 481 TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
T K TRVSNY P+L N NCTGSH NE G KI+SEDLT CKPRCL D PV
Sbjct: 481 TMKLSTRVSNYVPSLANVPNCTGSHCNETGCKIDSEDLT----------CKPRCLADCPV 540
Query: 541 PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
PSGNSDNVVRISG SPDEDSTL+C DE SSEN VE PNE++KN LTCSL E KK+EVPL
Sbjct: 541 PSGNSDNVVRISGTTSPDEDSTLHCLDEHSSENQVEKPNELVKNVLTCSLGEEKKVEVPL 600
Query: 601 G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
KAEN+LEESLND NYTSD LSHSCASGVV+KS+QNEEGGLHFSAS FKTE+KVSAIH
Sbjct: 601 DDKAENSLEESLNDYSNYTSDDLSHSCASGVVEKSTQNEEGGLHFSASKFKTENKVSAIH 660
Query: 661 SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
S LKKKGRRKCKKISEI PTLPPQI+IVS PG KT+ C+QLDMIEDQKSHIAD
Sbjct: 661 STLKKKGRRKCKKISEINPTLPPQINIVSTTPGKKTD-------CTQLDMIEDQKSHIAD 720
Query: 721 TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
TKNVDSHEK+ LSPISCHSERKGSK K FDS +GSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721 TKNVDSHEKSSFLSPISCHSERKGSKFKKKFDSLRGSKTRKKKLNECQIEDDDLLVSAII 780
Query: 781 RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
RNKDV+SSA GFSH+RKY KSRAKMN KSQK SCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781 RNKDVNSSAIGFSHIRKYLKSRAKMNCKSQKRSCKLLLRSLGNGEKNYKDGKWYAIGART 840
Query: 841 VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
VLSWLLDAGVISSNDIIQYQSPKD SVVKYGRITGDGIICNCCS++L+ISEFK HAGFKF
Sbjct: 841 VLSWLLDAGVISSNDIIQYQSPKDDSVVKYGRITGDGIICNCCSELLTISEFKCHAGFKF 900
Query: 901 NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
NR C NLFLDSGRPFMLCQLQAWSTEYKTR SKTRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901 NRPCLNLFLDSGRPFMLCQLQAWSTEYKTRTSKTRTVQVDEDDRNDDSCGICGDGGELIC 960
Query: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
CDNCPSTFHHSCLSIQELPEGNWYCLNCTCR CG LVN+EE SSSSDALKC QCEQKYHG
Sbjct: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRTCGGLVNYEETSSSSDALKCSQCEQKYHG 1020
Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
QCLKQ+DI+ GV SH+WFCS SCQKIYA LQS+LGL NQFANGFSW LLRCIH +QKILS
Sbjct: 1021 QCLKQKDIDPGVGSHLWFCSASCQKIYAGLQSRLGLINQFANGFSWMLLRCIHNEQKILS 1080
Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
T+RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV
Sbjct: 1081 TSRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200
Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVG 1256
AAIPSLVETWTEGFGF+ VE++EK SLH+FNLMVFPGT+LLKKALYVSGQ TETT G
Sbjct: 1201 AAIPSLVETWTEGFGFIPVEDDEKHSLHRFNLMVFPGTLLLKKALYVSGQNTETTQG 1238
BLAST of Cucsat.G8928.T22 vs. ExPASy TrEMBL
Match:
A0A6J1FC42 (LOW QUALITY PROTEIN: increased DNA methylation 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442744 PE=4 SV=1)
HSP 1 Score: 2064 bits (5347), Expect = 0.0
Identity = 1031/1257 (82.02%), Postives = 1104/1257 (87.83%), Query Frame = 0
Query: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
MDFQDDGFEGS NE+IIF+E+FFGNG+S SN+RCP +AFGYEHG CKINDASLCSSSE S
Sbjct: 1 MDFQDDGFEGSPNEDIIFKEIFFGNGASRSNERCPREAFGYEHGLCKINDASLCSSSELS 60
Query: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDAASD 120
VS + YSRN+KLDECYNATEN+RT SA+NS PCK VEG+D NA KR+K STD SD
Sbjct: 61 TVSSHLYSRNIKLDECYNATENVRTSSAANSFPCKLTPVEGNDENACAKRMKRSTDERSD 120
Query: 121 SVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVEMD 180
SVP+LV + +SSD I EPVS +C PAE CD ESFTFHIVESSR+GIISSCY L+ VE D
Sbjct: 121 SVPDLVMVMKSSDIIGEPVSTSCCPAEVCDSESFTFHIVESSRRGIISSCYLLKHFVERD 180
Query: 181 SNLADPDAVKQTSLNLEGHGEPNMVNKVSASPVSQESSMTRLLVANPSDKISEKFRSPLH 240
SN +PDA K+TSLNLEG+ EP+MV+KV ASPVSQESSMTRLLVA+PSD ++E+F SPL
Sbjct: 181 SNRGEPDASKRTSLNLEGNDEPSMVDKVGASPVSQESSMTRLLVASPSDTLNEQFGSPLQ 240
Query: 241 LEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
L VGQMK CPELD SL TDL RDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR
Sbjct: 241 LVVGQMKLQCPELDTSLMTDLKRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMETVYR 300
Query: 301 SPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKEMNQ 360
SPQG+ REFSKAWR CGELLFADRCSFVK+V SKEWTGIHQFLFDLSDTLL + KEMNQ
Sbjct: 301 SPQGKTIREFSKAWRVCGELLFADRCSFVKEVGSKEWTGIHQFLFDLSDTLLQVRKEMNQ 360
Query: 361 LGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLINED 420
LG TTSLA+CWVILDPYVVVVFI RKIG LR+GDLVRATCS+G+NG++KTD FVTL NED
Sbjct: 361 LGGTTSLAHCWVILDPYVVVVFIYRKIGTLRKGDLVRATCSIGVNGNNKTDTFVTLTNED 420
Query: 421 NGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHTEDG 480
+ C LSADKNASP+ + SPSAKSALTE LKD D GNCAFDEQ CDT FSNYY TED
Sbjct: 421 SSICSLSADKNASPLREQSPSAKSALTEVVLKDFDGGNCAFDEQACDTIFSNYYAQTEDA 480
Query: 481 TTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGDGPV 540
T K TRVSNY P L NG NCTGSH NEPG KI+SED+T CKPRCL D PV
Sbjct: 481 TMKLSTRVSNYVPRLVNGPNCTGSHCNEPGCKIDSEDIT----------CKPRCLADCPV 540
Query: 541 PSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLEVPL 600
PSGNSDNVVRISG SPDEDSTL+C DE SSEN VE PNE++KN LTCSL E KK+EVPL
Sbjct: 541 PSGNSDNVVRISGTTSPDEDSTLHCLDEHSSENQVEKPNELVKNVLTCSLGEEKKVEVPL 600
Query: 601 G-KAENNLEESLNDCPNYTSDGLSHSCASGVVQKSSQNEEGGLHFSASMFKTEDKVSAIH 660
KAEN+LEESLND NYTSD LSHSCASGVV+KS+QNEEGGLHFSAS FKTE+KVSAIH
Sbjct: 601 DDKAENSLEESLNDYSNYTSDDLSHSCASGVVEKSTQNEEGGLHFSASKFKTENKVSAIH 660
Query: 661 SILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQKSHIAD 720
S KKKGRRKCKKISEI PTLP QIDIVS PG KT+ CSQLDMIEDQK HIAD
Sbjct: 661 STSKKKGRRKCKKISEINPTLPSQIDIVSATPGKKTD-------CSQLDMIEDQKCHIAD 720
Query: 721 TKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLLVSAII 780
TKNVDSHEK+ LSPISCHSERKGSK K FDS +GSKTRKKKLNECQIEDDDLLVSAII
Sbjct: 721 TKNVDSHEKSSFLSPISCHSERKGSKFKKKFDSLRGSKTRKKKLNECQIEDDDLLVSAII 780
Query: 781 RNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYALGART 840
RNKDV+SSA GFSH+RKY KSRA MN KSQK SCKLLLRSLG+GEKNYKDGKWYA+GART
Sbjct: 781 RNKDVNSSAIGFSHIRKYLKSRANMNCKSQKRSCKLLLRSLGNGEKNYKDGKWYAIGART 840
Query: 841 VLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSHAGFKF 900
VLSWLLDAGVISSNDIIQYQSPKD SVVKYGRITGDGI+CNCCS++L+ISEFK HAGFKF
Sbjct: 841 VLSWLLDAGVISSNDIIQYQSPKDDSVVKYGRITGDGIVCNCCSELLTISEFKHHAGFKF 900
Query: 901 NRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDGGELIC 960
NR C NLFLDSGRPFMLCQLQAWSTEYKTR SKTRTV+VDEDDRNDDSCGICGDGGELIC
Sbjct: 901 NRPCLNLFLDSGRPFMLCQLQAWSTEYKTRTSKTRTVQVDEDDRNDDSCGICGDGGELIC 960
Query: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCEQKYHG 1020
CDNCPSTFHHSCLSIQELPEGNWYCLNCTCR CG LVN+EE SSSSDALKC QCEQKYHG
Sbjct: 961 CDNCPSTFHHSCLSIQELPEGNWYCLNCTCRTCGGLVNYEETSSSSDALKCSQCEQKYHG 1020
Query: 1021 QCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYDQKILS 1080
QCLKQ+DIN GV SHIWFCS SCQ IYA LQS+LGL NQ ANGFSW LLRCIH +QKILS
Sbjct: 1021 QCLKQKDINPGVGSHIWFCSASCQTIYAGLQSRLGLINQCANGFSWMLLRCIHNEQKILS 1080
Query: 1081 TARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFHGFYTV 1140
T+RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRL+FHGFYTV
Sbjct: 1081 TSRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLNFHGFYTV 1140
Query: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKVKKLVI 1200
ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKVKKLVI
Sbjct: 1141 ILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKVKKLVI 1200
Query: 1201 AAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETTVG 1256
AAIPSLVETWTEGFGF+ VE++EKQSLH+FNLMVFPGT+LLKKALYVSGQ TETT G
Sbjct: 1201 AAIPSLVETWTEGFGFIPVEDDEKQSLHRFNLMVFPGTLLLKKALYVSGQNTETTQG 1240
BLAST of Cucsat.G8928.T22 vs. ExPASy TrEMBL
Match:
A0A6J1CQ48 (LOW QUALITY PROTEIN: increased DNA methylation 1 OS=Momordica charantia OX=3673 GN=LOC111013131 PE=4 SV=1)
HSP 1 Score: 1991 bits (5158), Expect = 0.0
Identity = 1004/1262 (79.56%), Postives = 1099/1262 (87.08%), Query Frame = 0
Query: 1 MDFQDDGFEGSANEEIIFREVFFGNGSSHSNKRCPHKAFGYEHGPCKINDASLCSSSEPS 60
MDFQDD FEGSANE IIF+EVFFGN SSH NK+CP KAF YEH CKINDASLCSSS+ S
Sbjct: 1 MDFQDDDFEGSANEHIIFKEVFFGNSSSHFNKKCPCKAFSYEHESCKINDASLCSSSKFS 60
Query: 61 AVSIYSYSRNMKLDECYNATENIRTGSASNSLPCKRISVEGDDGNASGKRIKVSTDA--A 120
V +SYSRN+K DECY+A EN R+GS NSL CK SVE + NAS KRIK+STD
Sbjct: 61 TVCSHSYSRNIKPDECYSAIENNRSGSVRNSLQCKCTSVEDKNENASVKRIKLSTDEDEP 120
Query: 121 SDSVPNLVKLKQSSDSIREPVSANCSPAEECDPESFTFHIVESSRQGIISSCYRLRDLVE 180
SDS+P+L K+ SS+ IREP SA C PAE+CD ESFTFHIVESS QGIISSCY L++LVE
Sbjct: 121 SDSIPDLGKVMNSSEIIREPASAGCCPAEDCDCESFTFHIVESSSQGIISSCYLLKNLVE 180
Query: 181 MDSNLADPDAVKQTSLNLEGHGEPNMV-NKVSASPVSQESSMTRLLVANPSDKISEKFRS 240
MDSN+ DP K T+LNLEG+ E NMV NKV AS VSQESSMTRLLVA+PS + EKF S
Sbjct: 181 MDSNVGDPHVSKCTTLNLEGNDESNMVVNKVGASSVSQESSMTRLLVASPSVTLDEKFGS 240
Query: 241 PLHLEVGQMKSLCPELDASLKTDLSRDPRPLLHYHVVHLFIAAGWSIERVKRPCRRYMET 300
PLHL+VGQ + CPELD SLKTDL RDPRPLLHYHVVHLFIAAGWSIER KRPCRRY+ET
Sbjct: 241 PLHLDVGQTRFQCPELDTSLKTDLVRDPRPLLHYHVVHLFIAAGWSIERRKRPCRRYLET 300
Query: 301 VYRSPQGRAFREFSKAWRFCGELLFADRCSFVKDVESKEWTGIHQFLFDLSDTLLHIGKE 360
VYRSPQGR REFSKAWR CGELLFA+RCSFVK+V+SKEWTGIHQFLFDLSDTLL +GKE
Sbjct: 301 VYRSPQGRVIREFSKAWRICGELLFANRCSFVKEVDSKEWTGIHQFLFDLSDTLLQVGKE 360
Query: 361 MNQLGATTSLANCWVILDPYVVVVFIDRKIGPLRRGDLVRATCSVGINGSSKTDGFVTLI 420
MN LGATT LA+CWVILDPYVVVVFIDRKIG LR+GD+VRAT S+GINGS+KTD FVTL
Sbjct: 361 MNNLGATTMLAHCWVILDPYVVVVFIDRKIGTLRKGDVVRATRSIGINGSNKTDTFVTLT 420
Query: 421 NEDNGFCKLSADKNASPVHDNSPSAKSALTEAPLKDLDEGNCAFDEQTCDTSFSNYYGHT 480
N D+ C A+KN SP+HD+SPSAKSALTE LKDLD G AFDEQTCDTSFSNYYGHT
Sbjct: 421 NADS-MCNTFANKNTSPLHDHSPSAKSALTETVLKDLDGGYSAFDEQTCDTSFSNYYGHT 480
Query: 481 EDGTTKFPTRVSNYGPNLENGLNCTGSHFNEPGNKIESEDLTSSPAYFSRSTCKPRCLGD 540
E+GT F TRV +Y PN+ G +C GSH NE G KI+S+DL S PAY S STCK RCL D
Sbjct: 481 EEGTVNFSTRVCDYVPNVGTGPDCMGSHCNELGKKIDSKDLASLPAYLSGSTCKHRCLPD 540
Query: 541 GPVPSGNSDNVVRISGLASPDEDSTLYCSDEQSSENHVENPNEMMKNALTCSLVEGKKLE 600
G VPSGNSDNVVR+S SPD+DSTLYCSDEQSSEN VE PNEM KN L SL E ++E
Sbjct: 541 G-VPSGNSDNVVRVSCPVSPDQDSTLYCSDEQSSENQVEKPNEMDKNVLMSSLGEELQVE 600
Query: 601 VPLG-KAENNLEESLNDCPNYT-SDGLSHSCASGVVQKSSQNEE-GGLHFSASMFKTEDK 660
VPL K ENNL+ESLNDC NYT SD LSHSCASGVVQKS+QNEE GGL F+AS +TE+K
Sbjct: 601 VPLNDKVENNLDESLNDCQNYTTSDDLSHSCASGVVQKSTQNEEEGGLLFAASKLETENK 660
Query: 661 VSAIHSILKKKGRRKCKKISEIKPTLPPQIDIVSVAPGNKTEFWDIDGTCSQLDMIEDQK 720
VSA HSILKKK RRKCK+ISEI PT+PPQIDIV+V PG KT+ WDIDG CSQLDMIEDQK
Sbjct: 661 VSAAHSILKKKWRRKCKRISEINPTVPPQIDIVNVTPGKKTKLWDIDGNCSQLDMIEDQK 720
Query: 721 SHIADTKNVDSHEKNLSLSPISCHSERKGSKLNKNFDSHKGSKTRKKKLNECQIEDDDLL 780
S IADTKN D HEK+LSLSP+SC+SERKGSK K +DS +GSKTRKKKL ECQIEDDDLL
Sbjct: 721 SQIADTKNKDIHEKSLSLSPMSCYSERKGSKFKKIYDSLRGSKTRKKKLGECQIEDDDLL 780
Query: 781 VSAIIRNKDVSSSAAGFSHVRKYFKSRAKMNRKSQKSSCKLLLRSLGSGEKNYKDGKWYA 840
VSAIIRNKD SS AGFS VRK+ KSRAK +RKSQKSSCKLLLRSLG+GEK+YKDGKWY
Sbjct: 781 VSAIIRNKDFGSSTAGFSPVRKFLKSRAKRDRKSQKSSCKLLLRSLGNGEKSYKDGKWYN 840
Query: 841 LGARTVLSWLLDAGVISSNDIIQYQSPKDGSVVKYGRITGDGIICNCCSDILSISEFKSH 900
+GARTVLSWLLDAGVISSNDIIQYQ+PKD SVVKYGRITGDGIICNCCS++L+ISEFKSH
Sbjct: 841 IGARTVLSWLLDAGVISSNDIIQYQNPKDDSVVKYGRITGDGIICNCCSELLTISEFKSH 900
Query: 901 AGFKFNRACSNLFLDSGRPFMLCQLQAWSTEYKTRKSKTRTVEVDEDDRNDDSCGICGDG 960
+GFKF+R C NLFLDSG+PFMLCQLQAWSTEYKTR+S+T TV+VDEDDRNDDSCG+CGDG
Sbjct: 901 SGFKFSRPCLNLFLDSGKPFMLCQLQAWSTEYKTRRSRTGTVQVDEDDRNDDSCGVCGDG 960
Query: 961 GELICCDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGDLVNFEEISSSSDALKCFQCE 1020
GELICCDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICG LV++EE SSSSDALKC QCE
Sbjct: 961 GELICCDNCPSTFHHSCLSIQELPEGNWYCLNCTCRICGGLVDYEEASSSSDALKCSQCE 1020
Query: 1021 QKYHGQCLKQRDINSGVESHIWFCSGSCQKIYAALQSQLGLTNQFANGFSWTLLRCIHYD 1080
QKYHG+CLKQ+DI+ GVESHIWFCSGSCQK+YA LQS LGL NQF +G+SW LLRCIH D
Sbjct: 1021 QKYHGRCLKQKDIDPGVESHIWFCSGSCQKVYAGLQSLLGLINQFPDGYSWMLLRCIHND 1080
Query: 1081 QKILSTARLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSSFPRLDFH 1140
QK+LST RLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKS+FPRLDFH
Sbjct: 1081 QKVLSTQRLAMMAECNSRLVVALTIMEECFLSMVDPRTGIDMIPHLVYSWKSNFPRLDFH 1140
Query: 1141 GFYTVILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLMSFKV 1200
GFYTVILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEML+SFKV
Sbjct: 1141 GFYTVILEKDDVLLCVASIRVHGSELAEMPLIATCSKYRRQGMCRRLLNAIEEMLLSFKV 1200
Query: 1201 KKLVIAAIPSLVETWTEGFGFVTVENEEKQSLHKFNLMVFPGTVLLKKALYVSGQTTETT 1256
KKLVIAAIP+LVETWTEGFGF+ VE++EK+SLH+FNLMVFPGT+LLKKALY SGQ TE T
Sbjct: 1201 KKLVIAAIPTLVETWTEGFGFIPVEDDEKKSLHRFNLMVFPGTILLKKALYSSGQNTEKT 1260
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
F4IXE7 | 3.4e-199 | 37.22 | Increased DNA methylation 1 OS=Arabidopsis thaliana OX=3702 GN=IDM1 PE=1 SV=1 | [more] |
Q14839 | 2.1e-10 | 50.94 | Chromodomain-helicase-DNA-binding protein 4 OS=Homo sapiens OX=9606 GN=CHD4 PE=1... | [more] |
Q6PDQ2 | 2.1e-10 | 50.94 | Chromodomain-helicase-DNA-binding protein 4 OS=Mus musculus OX=10090 GN=Chd4 PE=... | [more] |
O43918 | 3.5e-10 | 55.32 | Autoimmune regulator OS=Homo sapiens OX=9606 GN=AIRE PE=1 SV=1 | [more] |
O97159 | 6.0e-10 | 45.45 | Chromodomain-helicase-DNA-binding protein Mi-2 homolog OS=Drosophila melanogaste... | [more] |
Match Name | E-value | Identity | Description | |
XP_004146095.1 | 0.0 | 99.60 | increased DNA methylation 1 [Cucumis sativus] >XP_031739517.1 increased DNA meth... | [more] |
XP_016903085.1 | 0.0 | 93.87 | PREDICTED: increased DNA methylation 1 isoform X1 [Cucumis melo] >XP_016903087.1... | [more] |
XP_038898710.1 | 0.0 | 87.68 | increased DNA methylation 1 [Benincasa hispida] >XP_038898711.1 increased DNA me... | [more] |
KAG7024783.1 | 0.0 | 81.66 | Increased DNA methylation 1, partial [Cucurbita argyrosperma subsp. argyrosperma... | [more] |
XP_022976401.1 | 0.0 | 82.90 | increased DNA methylation 1 isoform X2 [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
A0A1S4E4E1 | 0.0 | 93.87 | increased DNA methylation 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501805 P... | [more] |
A0A6J1IND9 | 0.0 | 82.90 | increased DNA methylation 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114768... | [more] |
A0A6J1IGS9 | 0.0 | 82.90 | increased DNA methylation 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114768... | [more] |
A0A6J1FC42 | 0.0 | 82.02 | LOW QUALITY PROTEIN: increased DNA methylation 1-like OS=Cucurbita moschata OX=3... | [more] |
A0A6J1CQ48 | 0.0 | 79.56 | LOW QUALITY PROTEIN: increased DNA methylation 1 OS=Momordica charantia OX=3673 ... | [more] |