Cucsat.G867.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G867.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	RNA-binding protein pno1
Location	ctg1: 3214568 .. 3216009 (+)
RNA-Seq Expression	Cucsat.G867.T2
Synteny	Cucsat.G867.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTAAACCCTAAAGCCTCCTCTTTTCTTCACAATCTCTTCTCCTCTTCCCTCTCACCACTTCAGGAACCCCCGCCGTCGCTCTACTCCTTTTTCACGACTTTCTACCCTCTCTCCATCTCAGGTATTCTCTTTATTTCCCCTTCTTTCACTTTCTACTTCTATTGCTTACAATCCATCAACGAACTTTAGTTCCACTCGAACCGATTTTTAATCAATCGTTTTTCAGTTTTTCCAGATTTTATATTGGATTATAGTACCTTTGAGAATCCCCTTCTCTTATTGACGTAGTAATGTTACTCATACTCGTTTGTAGCTAATCATAATTTTACTTGAATTCCTTCTGGCCTTCCCAGTAAGGCACTTCTGAAGTTTGTTTTGATTAAGCTTTACATATATACATAATATAACGACATCTGAACCTATATTTTGTTGCTTAGTTAAAGATTTTGGGTGTTTTCAGTGTGGAAACTCCGCACAATTATTCCATAATGCAGTCAAACGAAGCTTCCACTTCCATGGATGTGGAAACAATTTCAACTCAAACAAAAGATGTAGTTAGCACATTGCCTCCAAAACCACAGTTTGATCCTCTAAAGCCTCATGAAATGAATGATGGTCGAGTTCAATTCCGAAAGGTCTCTGTTCCACCCCATCGTTATTCCCCTCTAAAGAAAGCATGGATGGATATTTACACCCCAATTTACGACCAGATGAAAATCGACATCCGAATGAATCTCAAGGCACGGAAAGTCGAGTTGAAAACCAGAGCAGACACACCCGACATTAGTAACTTGCAGAAGTCTGCAGATTTTGTTCATGCTTTCATGCTTGGTTTCGATGTTATTGATGCCATTGCACTCTTACGTGTTGATGAACTCTATGTGGAGTCTTTCGAGATTAAAGATGTTAAAACGTTGCGAGGCGAGCACTTATCCCGAGCCATTGGTAGACTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAATGCCACCAAGACGAGGATAGTCATTGCAGATACTAAGATTCACATATTAGGATCCTTTGCAAATATCAAGATTGCTAGGGATTCTCTTTGCAGTCTCATTTTAGGGTCACCTGCTGGAAAAGTTTATTCAAAGCTAAGGGCAGTTACAGCCAGATTGGCAGAAAGGTTTTGATCTGTAATTTAATGTTTGTTTGTCTTGATAAATGAATTTTGCTTACACTAGTTGTTTGAGGAAGAGTACAACTTTATCGAAAAGCTTATGCATTGAAGTTTTGTAATCAATACACAAACCGGGTATCAGTTGATTCTTGGTATTTGCTGATGTTGTTTTAACATCGTTGATTGGTGTCCATTTGAGTCTTACCTATAAAGTGTGTACCATTTTATGAGAGAAAGAAAGGGGCATAGGTTTTATTTTCCTGTTTAACACAAGTGGGTTTCAAGATTGAT

Coding sequence (CDS)

ATGCAGTCAAACGAAGCTTCCACTTCCATGGATGTGGAAACAATTTCAACTCAAACAAAAGATGTAGTTAGCACATTGCCTCCAAAACCACAGTTTGATCCTCTAAAGCCTCATGAAATGAATGATGGTCGAGTTCAATTCCGAAAGGTCTCTGTTCCACCCCATCGTTATTCCCCTCTAAAGAAAGCATGGATGGATATTTACACCCCAATTTACGACCAGATGAAAATCGACATCCGAATGAATCTCAAGGCACGGAAAGTCGAGTTGAAAACCAGAGCAGACACACCCGACATTAGTAACTTGCAGAAGTCTGCAGATTTTGTTCATGCTTTCATGCTTGGTTTCGATGTTATTGATGCCATTGCACTCTTACGTGTTGATGAACTCTATGTGGAGTCTTTCGAGATTAAAGATGTTAAAACGTTGCGAGGCGAGCACTTATCCCGAGCCATTGGTAGACTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAATGCCACCAAGACGAGGATAGTCATTGCAGATACTAAGATTCACATATTAGGATCCTTTGCAAATATCAAGATTGCTAGGGATTCTCTTTGCAGTCTCATTTTAGGGTCACCTGCTGGAAAAGTTTATTCAAAGCTAAGGGCAGTTACAGCCAGATTGGCAGAAAGGTTTTGA

Protein sequence

MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF

Homology

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BLAST of Cucsat.G867.T2 vs. ExPASy Swiss-Prot
Match: Q9VR89 (RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 3.9e-66
Identity = 122/187 (65.24%), Postives = 155/187 (82.89%), Query Frame = 0


Query: 37  PHEMNDGRVQFRKVSVPPHRYSPLKKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADT 96
           P +    R + RKVSVPPHRYS LK+ WM I+TP+ + MK+ IR N+KAR+VEL+   +T
Sbjct: 53  PPKAKRARSELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPET 112

Query: 97  PDISNLQKSADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLS 156
           PDI+NLQ+ ADFV AF+ GF+V DA+ALLR+++L+VESFEIKDVKTLRG+H SRAIGRL+
Sbjct: 113 PDIANLQRGADFVRAFLCGFEVDDALALLRLEDLFVESFEIKDVKTLRGDHQSRAIGRLA 172

Query: 157 GKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAV 216
           GK G+TKF IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LRAV
Sbjct: 173 GKGGRTKFTIENVTKTRIVLADSKIHILGSYQNIQLARRAVCNLILGSPPSKVYGNLRAV 232

Query: 217 TARLAER 224
            +RL+ER
Sbjct: 233 ASRLSER 239

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BLAST of Cucsat.G867.T2 vs. ExPASy Swiss-Prot
Match: Q29IG6 (RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l(1)G0004 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 4.4e-65
Identity = 119/178 (66.85%), Postives = 152/178 (85.39%), Query Frame = 0


Query: 46  QFRKVSVPPHRYSPLKKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKS 105
           + RKVSVPPHRYS LK+ WM I+TP+ + MK+ IR N+KAR+VEL+   +TPDI+NLQK 
Sbjct: 60  ELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPETPDIANLQKG 119

Query: 106 ADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFA 165
           ADFV AF+ GF+V DA+ALLR+++L+VE+FEIKDVKTLRG+H SRAIGRL+GK G+TKF 
Sbjct: 120 ADFVKAFLCGFEVDDALALLRLEDLFVETFEIKDVKTLRGDHQSRAIGRLAGKGGRTKFT 179

Query: 166 IENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAER 224
           IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LR+V +RL+ER
Sbjct: 180 IENVTKTRIVLADSKIHILGSYQNIQLARRAICNLILGSPPSKVYGNLRSVASRLSER 237

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BLAST of Cucsat.G867.T2 vs. ExPASy Swiss-Prot
Match: A7RP64 (RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 5.7e-65
Identity = 118/196 (60.20%), Postives = 157/196 (80.10%), Query Frame = 0


Query: 29  KPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMDIYTPIYDQMKIDIRMNLKARKV 88
           +P F P+K  ++  G+ + RK+ VP HRY+PLK+ WM I+TP+ + +K+ IR NL +R V
Sbjct: 43  RPSFPPVKAQKLAGGKSETRKIPVPSHRYTPLKENWMKIFTPVVEHLKLQIRFNLGSRHV 102

Query: 89  ELKTRADTPDISNLQKSADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHL 148
           E++   +T DI  +QK+ADFV AF+LGF+V DA+AL+R+D+L++ESFEI DVK L+G+HL
Sbjct: 103 EIRASKETSDIGAVQKAADFVQAFILGFEVEDALALIRLDDLFLESFEIADVKPLKGDHL 162

Query: 149 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 208
           SRAIGR++GK GKTKF IEN TKTRIV+A+TKIHILGSF NIKIAR ++C+LILGSP  K
Sbjct: 163 SRAIGRVAGKGGKTKFTIENVTKTRIVLAETKIHILGSFQNIKIARTAICNLILGSPPSK 222

Query: 209 VYSKLRAVTARLAERF 225
           VY  +RAV +R AERF
Sbjct: 223 VYGNMRAVASRSAERF 238

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BLAST of Cucsat.G867.T2 vs. ExPASy Swiss-Prot
Match: Q6VEU3 (RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2)

HSP 1 Score: 247.7 bits (631), Expect = 1.3e-64
Identity = 123/211 (58.29%), Postives = 162/211 (76.78%), Query Frame = 0


Query: 15  ISTQTKDVVSTLPPK-PQFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMDIYTPIYD 74
           + TQT++  S+ P K P F  L   ++  G  + RKV VP HRY+PLK+ W+ I+TPI +
Sbjct: 42  MDTQTEESSSSAPVKRPHFPALSGDQLGGGVDEMRKVPVPSHRYTPLKENWLKIFTPIVE 101

Query: 75  QMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVIDAIALLRVDELYVE 134
            +++ +R NLK R+VE+KT  +T DI  L ++ADFV AF+LGF V DA+AL+R+DEL++E
Sbjct: 102 NLQLQVRFNLKTRQVEIKTCKETQDIGALTRAADFVKAFVLGFQVEDALALIRLDELFLE 161

Query: 135 SFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIA 194
           +F++ DVK L+G+HLSRAIGR++GK GKTKF IEN TKTRIV+ADTKIHILGSF NIK+A
Sbjct: 162 TFDVTDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMA 221

Query: 195 RDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           R ++C+LILGSP  KVY  LRAV  R AERF
Sbjct: 222 RTAICNLILGSPPSKVYGNLRAVATRSAERF 252

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BLAST of Cucsat.G867.T2 vs. ExPASy Swiss-Prot
Match: Q5F414 (RNA-binding protein PNO1 OS=Gallus gallus OX=9031 GN=PNO1 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.1e-63
Identity = 118/196 (60.20%), Postives = 156/196 (79.59%), Query Frame = 0


Query: 29  KPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMDIYTPIYDQMKIDIRMNLKARKV 88
           +P F PL    +  G+ + RKV VP +RY+PLK+ WM I+TPI + +++ IR NLK R V
Sbjct: 47  RPAFPPLPAAALGVGKGEVRKVPVPANRYTPLKENWMKIFTPIVEHLQLQIRFNLKTRNV 106

Query: 89  ELKTRADTPDISNLQKSADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHL 148
           E+KT ++T D+S L K+ADFV AF+LGF V DA+AL+R+D+L++ESFE+ DVK L+G+HL
Sbjct: 107 EIKTCSETKDLSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEVTDVKPLKGDHL 166

Query: 149 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 208
           SRAIGR++GK GKTKF IEN T+TRIV+AD+KIHILGSF NIK+AR ++C+LILGSP  K
Sbjct: 167 SRAIGRIAGKGGKTKFTIENVTRTRIVLADSKIHILGSFQNIKMARTAICNLILGSPPSK 226

Query: 209 VYSKLRAVTARLAERF 225
           VY  +RAV +R AERF
Sbjct: 227 VYGNIRAVASRAAERF 242

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BLAST of Cucsat.G867.T2 vs. NCBI nr
Match: XP_004148267.2 (RNA-binding protein pno1 [Cucumis sativus])

HSP 1 Score: 436 bits (1120), Expect = 7.64e-154
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120
           KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID
Sbjct: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120

Query: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

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BLAST of Cucsat.G867.T2 vs. NCBI nr
Match: XP_008449001.1 (PREDICTED: RNA-binding protein pno1 [Cucumis melo])

HSP 1 Score: 428 bits (1100), Expect = 8.56e-151
Identity = 219/224 (97.77%), Postives = 222/224 (99.11%), Query Frame = 0


Query: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQS EASTSM+VETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120
           KKAWM+IYTPIYDQMKID+RMNLKARKVELKTRADTPDISNLQK ADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDVRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Cucsat.G867.T2 vs. NCBI nr
Match: KAE8650098.1 (hypothetical protein Csa_010636 [Cucumis sativus])

HSP 1 Score: 436 bits (1120), Expect = 1.03e-150
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 205 MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 264

Query: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120
           KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID
Sbjct: 265 KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 324

Query: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 325 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 384

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 385 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 428

[Close]

BLAST of Cucsat.G867.T2 vs. NCBI nr
Match: XP_038904158.1 (RNA-binding protein pno1 [Benincasa hispida])

HSP 1 Score: 424 bits (1089), Expect = 4.07e-149
Identity = 218/224 (97.32%), Postives = 221/224 (98.66%), Query Frame = 0


Query: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQS EASTSM+VETISTQTK+VVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSIEASTSMEVETISTQTKNVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120
           KKAWMDIYTPI+DQMKIDIRMNLKARKVELKTRADTPDISNLQK ADFVH FMLGFDVID
Sbjct: 61  KKAWMDIYTPIFDQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHGFMLGFDVID 120

Query: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Cucsat.G867.T2 vs. NCBI nr
Match: XP_022932417.1 (RNA-binding protein pno1 [Cucurbita moschata])

HSP 1 Score: 422 bits (1084), Expect = 2.35e-148
Identity = 214/224 (95.54%), Postives = 222/224 (99.11%), Query Frame = 0


Query: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQS EASTSM+VET+ST+TK+VVSTLPPKPQFDPLKPHEMNDGR+QFRKVSVPPHRY+PL
Sbjct: 1   MQSTEASTSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPL 60

Query: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120
           KKAWM+IYTPIY+QMKIDIRMNLKARKVELKTRADTPDISNLQK ADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Cucsat.G867.T2 vs. ExPASy TrEMBL
Match: A0A0A0L7D9 (KH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G045150 PE=3 SV=1)

HSP 1 Score: 436 bits (1120), Expect = 3.70e-154
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120
           KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID
Sbjct: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120

Query: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Cucsat.G867.T2 vs. ExPASy TrEMBL
Match: A0A1S3BLP2 (RNA-binding protein pno1 OS=Cucumis melo OX=3656 GN=LOC103491005 PE=3 SV=1)

HSP 1 Score: 428 bits (1100), Expect = 4.14e-151
Identity = 219/224 (97.77%), Postives = 222/224 (99.11%), Query Frame = 0


Query: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQS EASTSM+VETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120
           KKAWM+IYTPIYDQMKID+RMNLKARKVELKTRADTPDISNLQK ADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDVRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Cucsat.G867.T2 vs. ExPASy TrEMBL
Match: A0A6J1EWL7 (RNA-binding protein pno1 OS=Cucurbita moschata OX=3662 GN=LOC111438772 PE=3 SV=1)

HSP 1 Score: 422 bits (1084), Expect = 1.14e-148
Identity = 214/224 (95.54%), Postives = 222/224 (99.11%), Query Frame = 0


Query: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQS EASTSM+VET+ST+TK+VVSTLPPKPQFDPLKPHEMNDGR+QFRKVSVPPHRY+PL
Sbjct: 1   MQSTEASTSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPL 60

Query: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120
           KKAWM+IYTPIY+QMKIDIRMNLKARKVELKTRADTPDISNLQK ADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Cucsat.G867.T2 vs. ExPASy TrEMBL
Match: A0A6J1HQ98 (RNA-binding protein pno1-like OS=Cucurbita maxima OX=3661 GN=LOC111465109 PE=3 SV=1)

HSP 1 Score: 418 bits (1075), Expect = 2.68e-147
Identity = 212/224 (94.64%), Postives = 221/224 (98.66%), Query Frame = 0


Query: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQ  EASTSM+VET+ST+TK+VVSTLPPKPQFDPLKPHEMNDGR+QFRKVSVPPHRY+PL
Sbjct: 1   MQPTEASTSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPL 60

Query: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120
           KKAWM+IYTPIY+QMKIDIRMNLKARKVELKTRADTPDISNLQK ADFVHAF+LGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFILGFDVID 120

Query: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Cucsat.G867.T2 vs. ExPASy TrEMBL
Match: A0A6J1KXN9 (RNA-binding protein pno1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498497 PE=3 SV=1)

HSP 1 Score: 417 bits (1073), Expect = 5.41e-147
Identity = 214/224 (95.54%), Postives = 218/224 (97.32%), Query Frame = 0


Query: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQS EASTSM+VE +STQ K VVSTLPPKP FDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120
           KKAWM+IYTPIY+QMKIDIRMNLKARKVELKTRADTPDISNLQK ADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Cucsat.G867.T2 vs. TAIR 10
Match: AT3G13230.1 (RNA-binding KH domain-containing protein )

HSP 1 Score: 352.1 bits (902), Expect = 3.4e-97
Identity = 178/216 (82.41%), Postives = 199/216 (92.13%), Query Frame = 0


Query: 7   STSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMD 66
           ST M+VET +  T      LPPKP F PLK HEM+DG+VQFRK++VPP+RYSPLKKAW+D
Sbjct: 4   STQMEVETATEGT----VPLPPKPTFKPLKAHEMSDGKVQFRKIAVPPNRYSPLKKAWLD 63

Query: 67  IYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVIDAIALLR 126
           IYTPIYDQMK+DIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFD+ DAI+LLR
Sbjct: 64  IYTPIYDQMKVDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDIPDAISLLR 123

Query: 127 VDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGS 186
           +DELYVESFEIKDVKTL+GEHLSRAIGRLSGK GKTKFAIEN+TKTRIVIADT+IHILG+
Sbjct: 124 MDELYVESFEIKDVKTLKGEHLSRAIGRLSGKGGKTKFAIENSTKTRIVIADTRIHILGA 183

Query: 187 FANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 223
           F+NIK+AR SLCSLI+GSPAGKVYSKLR+V+ARL E
Sbjct: 184 FSNIKVARSSLCSLIMGSPAGKVYSKLRSVSARLNE 215

The following BLAST results are available for this feature:

BLAST of Cucsat.G867.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9VR89	3.9e-66	65.24	RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV...	[more]
Q29IG6	4.4e-65	66.85	RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l...	[more]
A7RP64	5.7e-65	60.20	RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1	[more]
Q6VEU3	1.3e-64	58.29	RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2	[more]
Q5F414	1.1e-63	60.20	RNA-binding protein PNO1 OS=Gallus gallus OX=9031 GN=PNO1 PE=2 SV=1	[more]

BLAST of Cucsat.G867.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148267.2	7.64e-154	100.00	RNA-binding protein pno1 [Cucumis sativus]	[more]
XP_008449001.1	8.56e-151	97.77	PREDICTED: RNA-binding protein pno1 [Cucumis melo]	[more]
KAE8650098.1	1.03e-150	100.00	hypothetical protein Csa_010636 [Cucumis sativus]	[more]
XP_038904158.1	4.07e-149	97.32	RNA-binding protein pno1 [Benincasa hispida]	[more]
XP_022932417.1	2.35e-148	95.54	RNA-binding protein pno1 [Cucurbita moschata]	[more]

BLAST of Cucsat.G867.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7D9	3.70e-154	100.00	KH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G045150 PE=3 SV=...	[more]
A0A1S3BLP2	4.14e-151	97.77	RNA-binding protein pno1 OS=Cucumis melo OX=3656 GN=LOC103491005 PE=3 SV=1	[more]
A0A6J1EWL7	1.14e-148	95.54	RNA-binding protein pno1 OS=Cucurbita moschata OX=3662 GN=LOC111438772 PE=3 SV=1	[more]
A0A6J1HQ98	2.68e-147	94.64	RNA-binding protein pno1-like OS=Cucurbita maxima OX=3661 GN=LOC111465109 PE=3 S...	[more]
A0A6J1KXN9	5.41e-147	95.54	RNA-binding protein pno1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]

BLAST of Cucsat.G867.T2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13230.1	3.4e-97	82.41	RNA-binding KH domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004087	K Homology domain	SMART	SM00322	kh_6	coord: 129..202 e-value: 1.7E-4 score: 31.0
IPR036612	K Homology domain, type 1 superfamily	GENE3D	3.30.1370.10	K Homology domain, type 1	coord: 128..224 e-value: 1.1E-22 score: 81.9
IPR036612	K Homology domain, type 1 superfamily	SUPERFAMILY	54791	Eukaryotic type KH-domain (KH-domain type I)	coord: 129..223
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..40
None	No IPR available	PANTHER	PTHR12826	RIBONUCLEASE Y	coord: 7..224
None	No IPR available	CDD	cd00105	KH-I	coord: 149..195 e-value: 0.00998374 score: 31.7616
IPR039912	Pre-rRNA-processing protein PNO1-like	PANTHER	PTHR12826:SF13	RNA-BINDING PROTEIN PNO1	coord: 7..224

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G867	Cucsat.G867	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G867.T2.E1	Cucsat.G867.T2.E1	exon
Cucsat.G867.T2.E2	Cucsat.G867.T2.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G867.T2.C1	Cucsat.G867.T2.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G867.T2	Cucsat.G867.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003723	RNA binding