Cucsat.G849.T27 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G849.T27
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionglutamyl-tRNA(Gln) amidotransferase subunit A
Locationctg1: 2907068 .. 2912979 (+)
RNA-Seq ExpressionCucsat.G849.T27
SyntenyCucsat.G849.T27
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAAAGTCTTTTTTCCATCAAAATAATCTCATTTTTTTTTGGCAATTTCCCATCCCAATTCAGTGCCGGCGGTGACTCAGTGGTCACTCAGCAGTCAGCCACGCCGTTCGACAAGTGTCCTACAATGGCGTCGACTCCTACTATTAACTGCTTCGTTGTCTTCTTCTTCTTCTTCTTCTTCACAGTTTCGGTTCGATCTTCCATTTCCTCCCACTTCTCTTCTTTCCCTCAATGTTCTTGGCGTCGCTTCACACTAATGGTAACTTCTCTTCTGCTTTTACATCGCAATTTTCTCCCTATTATGCTTTTTTGTTCTTCTTTTGTTATCGGAAGGAATCATCACTTCAACGTGTTCTGGAAAAGCCCCAATTAGATACTATACCCTGCTTGTTTAAATCTTAGATTATGATTTTCCTATCCACATATTGTATAAAATAAATTACTTTTTGTTTTTCATTAGAGATGAAGAGAGGGGATGGAGGCTAGAGCTAAGAGGATATGAAGGGTTAATAATAATAACATTAATGATACTTGTATATTAATATATGATAATAATTTCAAATTGGTTGCCAAGAAGAGTATGACTTCACCAATTTAATTTAGTTTTTCTTTTTACAATTTAGGGGTAAGGGATTGGACCTCCAAGTTATATGTCATTCTTGATATTCTTATTAAGCTTTGCTATTTTTCCCACCAATTTTGTATTATTATCATATATTGCATTGCAATTATTATTAATGTTTATTATTGTTTGGGTTAGTTGCTTAAATGACATTTAAGCATAACTCAATAAATAAGTAAAATTAGAATATTTAGCCCAAAGATAAAATAATTACATATATAGCTCAAAAGAAAAAAAGTTGGGAAAAAAACTCACACCTATAAATGTTATCACTTATAGCTACTATCTAACGGTAACTTTAAATTTGAGAAATGTGTTCATGAATGGATATCAACTAAGAAGCACAAACACCGATAGGTCGATTTCTAAAATTTTAGGATACTGACACGTGTAAGCACAATGAATTGCTATCGTTGACAGCTATCAGGGATGGAAACATGTCCATTGATAGCTATTAGCAATGAAAACATGTGCATTGAAGGGTACACCTAGAATTCTAAAAATTTTACAAGTTGATTGGTATTTGTTATACTTACAACTATTTCAAAGTTACGTTATATTTTTATACTATTTTCCTAATTGTGTTATTTGCTATAATTTTCTTTATTGTTATGGAGCATTTTCCAATATAGCAAAAACTTGGTTAAATTTCTCACTCAGAACTTGTCATCTGACATGTTATTTTCCTAAACCTGGGGATAATTGTGAGACCATTTAAAAGTATTTTACAAATCTCAGGAATTGAAGGACAACTTTTAGAACTTCGGATTAGCTCGTTTACACGGTTGGAAATTTAGAAACTTAGCTCTTGCTATAACTTTCAAAAGTAAATTGATAGATGTCGGTTGAGGCTCTTGGTTGCATTGAGGTGGGCCAAATTTTACTCGATTCTTTATTTATAGAGAAAGCCAAAATTTACCATAGAGAGTAATTTTGTAATTCCTAATCTAGGTCCCAAATGAAAGGACCAAACTCACAATGTTCTGTTTTTTTATTCAAGTGCAAAAACTATCACAAACACTAATTATAATAAGTGTTTAGAAGTTTGATATTCACTAATATATTAAGCTCTCAGTCTTACAGGAATATCGATGTAAATTAAAATGTCGATATCAATATTTTGTAAAATAATAATAAAAAGTAAAAAGATTGTTTAAATGAATCAGTAAACATTTCTCATTCATTAATAAAATGCTTGTTATTCGTATTATATGCTATTTATATTTGCTACAATTTGTTGTTTGTGTTTCATCAGACCATATAATAAAAATGTTGACATGTGGACAAAAATTTAGTACTCTACTTCCAGGGCATTTTGAAGTCTTTTTTTGTATCTTTTTTTTAAAAACTATTCTTTCTTGATAAATGTTAAACAGATGGGAACTTATAAAAAATTCTGGAAACTTTTGTATGAAAAAGTTGGGGTTCATAAGCCATATTCTTCCTTTCTGCAGGTAACATTTGTTAATTACTCTGCTAACCAATTGTTCTACTCCATTTTCGTCTTGTTGTTTCTTTTCATGTTCTTTTCATCAGGACAGCAAGAAAGACTCTGAGTGTTTGGAAAATATGGGGAGAACATCCGTTCTGAAATGTGTATTTAGAGCACTTGATGCAGATTTCTTCAATAACACCAAGGTTCAACAAAGCACCAATTGTTTTCATGTAGTTATATATTGTGTTGTCTTTTACACTCATGTAACGTTGAGAAACTTTTGTTTTTGAGTAGAGGGATGAGGTTTTAAAGGGAGCTGAAGAACTTAATGTGCCTATAATCAGAGCCAACCGGAAGTTAGTTGCTTCTGAAAATGGAGGTCTGCATAATCCTTCCTCTTTGGTGTTCAACCCTGAGTGGGCAAATGAAAACGTTCGGCATAAGAGCAAAAGATTCTGTTATCCTCCTGTTTCTGGAATAAAGAGACCAAACAACGAAGACGATATTGCTTTCATGAGTGTACGTCTGCACTGATCTTTTTAGTTTGAATTGAAGCTAAATATGTACTTTAGTCTCGAGTGTTTGAGTTTTTTTTTTTCCTTTTTCCCTCTGATTTGGAAAAAAGGCCCACAGTTACAAATGTTGGAATTTCGTTGATCGAGTAAAAAATATGTTTTGCTTGATGTAACCATAAAATAAAATAGTATTCATTTTATATCCCTTAGCAAGTAGTAACTTGTTTGGCCCAAAATTGCTTATCGTTCTCTAAAATCCATCCATCCAAATATTGGAATACTATCAAATTAAAACCTAATTGTTTTTAGATTCATATGATCCTTCTTTTAACATTAGGGACTAAAGGGAGTGATGGTTGAATTCTATTAGGTTCTTGAACTTGGAGAACTGATTAAAACGGAGCAGATTTCATCTCAGGAGCTTGTTCGAATTTTTCTTCAGAGATTGAAAAGGTATATAAAAAACCAACTTACAGTCTTTCGGATCGAAGTTGAAGTTCTTATTTAAAAATTTTCTTTAAACAATATCAGGTACAACCATGTTCTTGAAGCTGTAGTTTCTTTTACTGAAGAACTGGCTTACAAACAAGCAAAAGAGGCTGATGAACTGTTTGCTCAAGGAGTGTATTTAGGTTCGTGTCTTTTATGATGATAATTTTTTGCCCTACTTCAATGAAAGTCGTTGATTTGGGTGATGCATTTATGAATTGTAGTTCACTGTTTGACTTTCGACTTTATTTTTCAAATGCGGTGTTAAGTTTATACTTCACTTTGAAATAAAATATTATTCTTGCAGAGTTAAATTTATACCTTTAAACTTAGAAACAAAAAGTTCTTGTTTTGCAGGTCCACTGCATGGAATTCCATATGGGTTGAAGGATATAATATCTGTTCCAGGATACAAAACAACTTGGGGTTCAAAAAGTTTTAAAGAACAAGTAATTGATGTTGAAGCTTGGGTTTATAAGCAGTACGAAAAAAATGACTAATTTATCTAAGTATATTAATTGACATCTTTGCCAATAAATCATGAATCATTTGGTTTTTAACATCTTTACAAACAAACCGCGAATCCTTTGGTTTTGCAGGTTGAAGTCAGCAGGTGCTGTTCTTGTTGCAAAACTTGTTACCGGATCACTTGCATATGATGACATCTGGTTTGGTGGTCGTACGAGGAACCCGTGGAACATTGAGGAGTTTAGTACAGGGTCATCAGCTGGACCTGCAGCATGTACTTCTGCAGGTACGATTTAGACTTGGAGCTGATGGTTTCACCACAAAATGTTTTATTTTTCTTAGCAAGTCTTAGAACATCTCCATTACAACATCATCTTGAGTATAATATTTTTGGTTGAATTCCAGCAATTTTCATTTCAACTTGAGACTTCATTGCCTTAACCATTGATTTGCTTCAGGTGTTAGGTTCTTGTGTTTCTTAAGAAAAAAAGAGCTTGTCAAGTTTGTCATAGTTTCTTGCTATAAGAATATTTCCTTCACAATTTTGGATAATGAACCCAGAGGAGATGGGGCTTTATATAATGCATTGCCCCCTTGTTTACTAGGTATGGTTCCATTTGCAATTGGATCAGAGACAATTGGTTCGATGACTTATCCTGCAGCTCGATGTGGAGTTACTGCAATCCGGCCAACATTTGGAACAGTTGGTCGCAGTGGAGTGATGAGTATTTCTGAAAGCTTGGTAGTAGCCACAAGCCCTTTTTGCTTTGTTTCTCAACTCGCCTTTTGTATTTATTCTGCTTTTATTTAACAGGATAAGCTTGGTCCATTTTGTAGAAACGCGGTTGATTGTGCAGTCGTTTTGGATGTCATTAGAGGGAAGGATCCACATGACCTCTCCTCAGTTGAGAGTTCTCTTGATGATCCATTCTCCATTGACATTTCTAAACTTACTGTTGGATATCTTGACGATGCTGACATGGAGGTCAGTAACTCCATCAGAGTAGAGCCTTCTTCTTTGACATCTTTGATGTTAACCCAACTTTTAAAATGTTTATTATAGGTTGTTCGGATTCTTTCCTCTAAGGGTGTCAATATGGTTCCATTCAACTTGTCTTATTCTGTTGATTCTGTTCAAGGCATTATTAACTTCACTATGGATGTTGATATGTTGGCTCATTTTGACGAATGGCAACGTTTGGGGCTTGATGATGAATATGAAGCTCAAGACCAATGGCCAACTGAACTCCGTCGTGCACGTTTAATCCCAGCCGTAGATTATGTTCAGGTATTGTCTACATAAAGACTCAGTCTTCATTTATCAATAAACTTGATCCCGATTAGTTCATTAGTTTACACGAGCAATAAAATTCATTAAAAACTTTACTATACTACTTTTTTCCATAGACGGAAATTGTTTGTTGCACATTCTAATGTAAGACTAAATTAATACTTATTGAAGTTCAGGAAAAACGTCGGACGAGTATAGGAAGTTAATAGTTACCAACCAGACCGTTGTGACCAATTTTTTTTTTTTAACCCTTGATTATCCAATCCATGCACAGGCACAGAGAGCAAGGGGAAAGCTAATTAGAGAAGTTAGAGAAAGTTTCAACGTTGATGCTTTAATTGGCAATGCCACTGACTGGGAGAAAGTTTGTATGGGAAATCTTGTTGGTCTGCCTATTGTTGTTGTTCCAACAGGTTTCAAAAACATCTCAAACCCACCGTCAACTGGCACCACAAGAAGAAAAACCACTATTACCACTGGCATTTATGCTCCTCCTCACAGAGATCACATTGTATGCAACTTTTTCTTTTCTTGGAATAAAAAAAATAAGACTCCTTTTGGTTTTTGTGGGTAACTACTTATTGTATTTGATGAATGATTGTTTGTGTTGTAGGCTTTAGCTTTGGCTATGGCTTACCAATCTGCAACTGATCACCACAGAGCAAAGCCACCAATAGATGACTTGGGGCCCAGTGACGTTCTTCCAGATCCACCTTTGGTATCTATTCCTCCAAGGTTATTGCACCTTTGAACATGAATCTGCACCTCTTTTCCCTTGTCTTATCTTACATTTTGGAGACTTTTGTCTTCTGTTTTTTCTTTTTGGCTATAGAGAAAAACAGAAATATAGTTTAGTCCCGAATGTTAATATGCATACATCTACGACTGCGACATTCTCGATGTCAGAGTTTTTGTATGTAAATGCTGCTTCTAGTTCAAGCTCTATCATCCATTAAGTAATATAATCAATGTTATGGAATTAAAACTAGTTCATATTTTCACGTCTTTCCCTTTTTATTTCTGAAAATTGTTCCAAAGCCACCTCTGTTTACAGTTTCTACGATTGTTGCTGTTGAGAGATTTGGGGTTC

Coding sequence (CDS)

CCAAAAAGTCTTTTTTCCATCAAAATAATCTCATTTTTTTTTGGCAATTTCCCATCCCAATTCAGTGCCGGCGGTGACTCAGTGGTCACTCAGCAGTCAGCCACGCCGTTCGACAAGTGTCCTACAATGGCGTCGACTCCTACTATTAACTGCTTCGTTGTCTTCTTCTTCTTCTTCTTCTTCACAGTTTCGGTTCGATCTTCCATTTCCTCCCACTTCTCTTCTTTCCCTCAATGTTCTTGGCGTCGCTTCACACTAATGATGGGAACTTATAAAAAATTCTGGAAACTTTTGTATGAAAAAGTTGGGGTTCATAAGCCATATTCTTCCTTTCTGCAGGACAGCAAGAAAGACTCTGAGTGTTTGGAAAATATGGGGAGAACATCCGTTCTGAAATGTGTATTTAGAGCACTTGATGCAGATTTCTTCAATAACACCAAGAGGGATGAGGTTTTAAAGGGAGCTGAAGAACTTAATGTGCCTATAATCAGAGCCAACCGGAAGTTAGTTGCTTCTGAAAATGGAGGTCTGCATAATCCTTCCTCTTTGGTGTTCAACCCTGAGTGGGCAAATGAAAACGTTCGGCATAAGAGCAAAAGATTCTGTTATCCTCCTGTTTCTGGAATAAAGAGACCAAACAACGAAGACGATATTGCTTTCATGAGTGTTCTTGAACTTGGAGAACTGATTAAAACGGAGCAGATTTCATCTCAGGAGCTTGTTCGAATTTTTCTTCAGAGATTGAAAAGGTACAACCATGTTCTTGAAGCTGTAGTTTCTTTTACTGAAGAACTGGCTTACAAACAAGCAAAAGAGGCTGATGAACTGTTTGCTCAAGGAGTGTATTTAGGTCCACTGCATGGAATTCCATATGGGTTGAAGGATATAATATCTGTTCCAGGATACAAAACAACTTGGGGTTCAAAAAGTTTTAAAGAACAAGTAATTGATGTTGAAGCTTGGGTTTATAAGCAGTTGAAGTCAGCAGGTGCTGTTCTTGTTGCAAAACTTGTTACCGGATCACTTGCATATGATGACATCTGGTTTGGTGGTCGTACGAGGAACCCGTGGAACATTGAGGAGTTTAGTACAGGGTCATCAGCTGGACCTGCAGCATGTACTTCTGCAGGTATGGTTCCATTTGCAATTGGATCAGAGACAATTGGTTCGATGACTTATCCTGCAGCTCGATGTGGAGTTACTGCAATCCGGCCAACATTTGGAACAGTTGGTCGCAGTGGAGTGATGAGTATTTCTGAAAGCTTGAAACGCGGTTGA

Protein sequence

PKSLFSIKIISFFFGNFPSQFSAGGDSVVTQQSATPFDKCPTMASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMMGTYKKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLKRG
Homology
BLAST of Cucsat.G849.T27 vs. ExPASy Swiss-Prot
Match: Q3AD36 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=gatA PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 5.4e-32
Identity = 78/209 (37.32%), Postives = 118/209 (56.46%), Query Frame = 0

Query: 217 DIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADEL 276
           ++ ++S  E+ E IK ++IS+ E+ +    R++     ++A V+ T EL  K A+E DE 
Sbjct: 2   ELYYLSAKEITEKIKAKEISAVEVAKATFDRIEAVEPKIQAYVTVTRELGLKMAREVDEK 61

Query: 277 FAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAK 336
            A+G   GPL G+P  +KD +S  G +TT  SK  +  +   +A V ++LK AGAV   K
Sbjct: 62  IARGEDPGPLAGVPVAIKDNMSTAGIRTTCSSKILENYIPPYDATVVEKLKEAGAVFTGK 121

Query: 337 LVTGSLAYDDIWFGGR---TRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTY 396
                 A        R   TRNPW++E    GSS G AA  +AG    A+GS+T GS+  
Sbjct: 122 TNLDEFAMGSSTENSRFFPTRNPWDLERVPGGSSGGSAASVAAGEAVVALGSDTGGSIRQ 181

Query: 397 PAARCGVTAIRPTFGTVGRSGVMSISESL 423
           PAA CG+  ++PT+G V R G+++ + SL
Sbjct: 182 PAAFCGIVGLKPTYGAVSRYGLVAFASSL 210

BLAST of Cucsat.G849.T27 vs. ExPASy Swiss-Prot
Match: B6J4H5 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain CbuK_Q154) OX=434924 GN=gatA PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.3e-30
Identity = 80/204 (39.22%), Postives = 116/204 (56.86%), Query Frame = 0

Query: 222 SVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGV 281
           ++ EL + ++ + ISS EL + FL R+K  N  L + +S TEE A  QAK AD   A+G 
Sbjct: 5   TIAELKQDLRDKTISSVELTQHFLDRIKTINPTLNSFISITEEYALTQAKAADARLAKGE 64

Query: 282 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGS 341
               L GIP   KDI      KT+ GSK     +   +A V +QL  AGA+L+ K     
Sbjct: 65  ATS-LTGIPIAQKDIFCTKDIKTSCGSKMLDNFIAPYDATVVEQLNKAGAILIGKTNMDE 124

Query: 342 LAY---DDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARC 401
            A    ++  + G  +NPW++E    GSS G AA  +A +VP A G++T GS+  PAA C
Sbjct: 125 FAMGSSNENSYFGAVKNPWDLERVPGGSSGGSAAAVAARLVPGATGTDTGGSIRQPAALC 184

Query: 402 GVTAIRPTFGTVGRSGVMSISESL 423
           G+T ++PT+G V R G+++ + SL
Sbjct: 185 GITGLKPTYGRVSRYGMIAFASSL 207

BLAST of Cucsat.G849.T27 vs. ExPASy Swiss-Prot
Match: B6IZ25 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain CbuG_Q212) OX=434923 GN=gatA PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.3e-30
Identity = 80/204 (39.22%), Postives = 116/204 (56.86%), Query Frame = 0

Query: 222 SVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGV 281
           ++ EL + ++ + ISS EL + FL R+K  N  L + +S TEE A  QAK AD   A+G 
Sbjct: 5   TIAELKQDLRDKTISSVELTQHFLDRIKTINPTLNSFISITEEYALTQAKAADARLAKGE 64

Query: 282 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGS 341
               L GIP   KDI      KT+ GSK     +   +A V +QL  AGA+L+ K     
Sbjct: 65  ATS-LTGIPIAQKDIFCTKDIKTSCGSKMLDNFIAPYDATVVEQLNKAGAILIGKTNMDE 124

Query: 342 LAY---DDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARC 401
            A    ++  + G  +NPW++E    GSS G AA  +A +VP A G++T GS+  PAA C
Sbjct: 125 FAMGSSNENSYFGAVKNPWDLERVPGGSSGGSAAAVAARLVPGATGTDTGGSIRQPAALC 184

Query: 402 GVTAIRPTFGTVGRSGVMSISESL 423
           G+T ++PT+G V R G+++ + SL
Sbjct: 185 GITGLKPTYGRVSRYGMIAFASSL 207

BLAST of Cucsat.G849.T27 vs. ExPASy Swiss-Prot
Match: A9KBI0 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain Dugway 5J108-111) OX=434922 GN=gatA PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.3e-30
Identity = 80/204 (39.22%), Postives = 116/204 (56.86%), Query Frame = 0

Query: 222 SVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGV 281
           ++ EL + ++ + ISS EL + FL R+K  N  L + +S TEE A  QAK AD   A+G 
Sbjct: 5   TIAELKQDLRDKTISSVELTQHFLDRIKTINPTLNSFISITEEYALTQAKAADARLAKGE 64

Query: 282 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGS 341
               L GIP   KDI      KT+ GSK     +   +A V +QL  AGA+L+ K     
Sbjct: 65  ATS-LTGIPIAQKDIFCTKDIKTSCGSKMLDNFIAPYDATVVEQLNKAGAILIGKTNMDE 124

Query: 342 LAY---DDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARC 401
            A    ++  + G  +NPW++E    GSS G AA  +A +VP A G++T GS+  PAA C
Sbjct: 125 FAMGSSNENSYFGAVKNPWDLERVPGGSSGGSAAAVAARLVPGATGTDTGGSIRQPAALC 184

Query: 402 GVTAIRPTFGTVGRSGVMSISESL 423
           G+T ++PT+G V R G+++ + SL
Sbjct: 185 GITGLKPTYGRVSRYGMIAFASSL 207

BLAST of Cucsat.G849.T27 vs. ExPASy Swiss-Prot
Match: A9N8Z4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain RSA 331 / Henzerling II) OX=360115 GN=gatA PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.3e-30
Identity = 80/204 (39.22%), Postives = 116/204 (56.86%), Query Frame = 0

Query: 222 SVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGV 281
           ++ EL + ++ + ISS EL + FL R+K  N  L + +S TEE A  QAK AD   A+G 
Sbjct: 5   TIAELKQDLRDKTISSVELTQHFLDRIKTINPTLNSFISITEEYALTQAKAADARLAKGE 64

Query: 282 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGS 341
               L GIP   KDI      KT+ GSK     +   +A V +QL  AGA+L+ K     
Sbjct: 65  ATS-LTGIPIAQKDIFCTKDIKTSCGSKMLDNFIAPYDATVVEQLNKAGAILIGKTNMDE 124

Query: 342 LAY---DDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARC 401
            A    ++  + G  +NPW++E    GSS G AA  +A +VP A G++T GS+  PAA C
Sbjct: 125 FAMGSSNENSYFGAVKNPWDLERVPGGSSGGSAAAVAARLVPGATGTDTGGSIRQPAALC 184

Query: 402 GVTAIRPTFGTVGRSGVMSISESL 423
           G+T ++PT+G V R G+++ + SL
Sbjct: 185 GITGLKPTYGRVSRYGMIAFASSL 207

BLAST of Cucsat.G849.T27 vs. NCBI nr
Match: XP_011650445.1 (uncharacterized protein LOC101214338 isoform X1 [Cucumis sativus] >KAE8650086.1 hypothetical protein Csa_011729 [Cucumis sativus])

HSP 1 Score: 760 bits (1963), Expect = 3.31e-272
Identity = 380/382 (99.48%), Postives = 381/382 (99.74%), Query Frame = 0

Query: 43  MASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMMGTYKKFWKLLYEKV 102
           MASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMMGTYKKFWKLLYEKV
Sbjct: 1   MASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMMGTYKKFWKLLYEKV 60

Query: 103 GVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPI 162
           GVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPI
Sbjct: 61  GVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPI 120

Query: 163 IRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMS 222
           IRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMS
Sbjct: 121 IRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMS 180

Query: 223 VLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVY 282
           VLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVY
Sbjct: 181 VLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVY 240

Query: 283 LGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL 342
           LGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL
Sbjct: 241 LGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL 300

Query: 343 AYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTA 402
           AYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTA
Sbjct: 301 AYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTA 360

Query: 403 IRPTFGTVGRSGVMSISESLKR 424
           IRPTFGTVGRSGVMSISESL +
Sbjct: 361 IRPTFGTVGRSGVMSISESLDK 382

BLAST of Cucsat.G849.T27 vs. NCBI nr
Match: XP_011650446.1 (uncharacterized protein LOC101214338 isoform X2 [Cucumis sativus])

HSP 1 Score: 691 bits (1784), Expect = 2.14e-245
Identity = 354/382 (92.67%), Postives = 355/382 (92.93%), Query Frame = 0

Query: 43  MASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMMGTYKKFWKLLYEKV 102
           MASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLM               
Sbjct: 1   MASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLM--------------- 60

Query: 103 GVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPI 162
                      DSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPI
Sbjct: 61  -----------DSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPI 120

Query: 163 IRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMS 222
           IRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMS
Sbjct: 121 IRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMS 180

Query: 223 VLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVY 282
           VLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVY
Sbjct: 181 VLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVY 240

Query: 283 LGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL 342
           LGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL
Sbjct: 241 LGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL 300

Query: 343 AYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTA 402
           AYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTA
Sbjct: 301 AYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTA 356

Query: 403 IRPTFGTVGRSGVMSISESLKR 424
           IRPTFGTVGRSGVMSISESL +
Sbjct: 361 IRPTFGTVGRSGVMSISESLDK 356

BLAST of Cucsat.G849.T27 vs. NCBI nr
Match: XP_016900589.1 (PREDICTED: glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo])

HSP 1 Score: 660 bits (1702), Expect = 3.40e-233
Identity = 325/337 (96.44%), Postives = 331/337 (98.22%), Query Frame = 0

Query: 88  MGTYKKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTK 147
           MGTYKKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTS+LKCVFRA DADFFNNTK
Sbjct: 1   MGTYKKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTSILKCVFRAFDADFFNNTK 60

Query: 148 RDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVS 207
           RDEVLKGAEE+NVP IRANRKLVASENGGLHNPS LVFNPEWANEN RHKSKRFCYPPV 
Sbjct: 61  RDEVLKGAEEVNVPTIRANRKLVASENGGLHNPSPLVFNPEWANENARHKSKRFCYPPVH 120

Query: 208 GIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAY 267
           G+KRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAY
Sbjct: 121 GVKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAY 180

Query: 268 KQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLK 327
           KQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLK
Sbjct: 181 KQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLK 240

Query: 328 SAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSET 387
           SAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEF+TGSSAGPAACTSAGMVPFAIGSET
Sbjct: 241 SAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSET 300

Query: 388 IGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLKR 424
           +GSMTYPAARCGVTAIRPTFGTVGRSGVMSISESL +
Sbjct: 301 VGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDK 337

BLAST of Cucsat.G849.T27 vs. NCBI nr
Match: XP_038905194.1 (glutamyl-tRNA(Gln) amidotransferase subunit A [Benincasa hispida])

HSP 1 Score: 616 bits (1588), Expect = 2.34e-215
Identity = 317/372 (85.22%), Postives = 333/372 (89.52%), Query Frame = 0

Query: 53  VVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMMGTYKKFWKLLYEKVGVHKPYSSFL 112
           V FFFFFFF  +V SS   H    P  S  RFT MMGTYKKFWKL+ EKVGVH     F 
Sbjct: 10  VNFFFFFFFFFAVPSSPIFH--VLPSNSCPRFTPMMGTYKKFWKLMCEKVGVHSHTPPFC 69

Query: 113 QDSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVAS 172
             SKKD+EC+   G+TS+LKCVFRA DADFFNNTK DEVLKGAEE N+P IRANRKLVAS
Sbjct: 70  --SKKDTECV---GKTSLLKCVFRAFDADFFNNTKMDEVLKGAEEFNLPTIRANRKLVAS 129

Query: 173 ENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKT 232
            NGGLHNPS LVFNPEWANEN+RHKSKRFCYP VSG+KRP NE+DIAFMSVLELGELIKT
Sbjct: 130 RNGGLHNPSPLVFNPEWANENIRHKSKRFCYPSVSGVKRPKNEEDIAFMSVLELGELIKT 189

Query: 233 EQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYG 292
           +QISSQELVRIFLQRLKRYNHVL+AVVSFTEELAYKQAKEADELFA+G YLGPLHGIPYG
Sbjct: 190 KQISSQELVRIFLQRLKRYNHVLKAVVSFTEELAYKQAKEADELFARGTYLGPLHGIPYG 249

Query: 293 LKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGR 352
           LKDIISVPGY+TTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGR
Sbjct: 250 LKDIISVPGYETTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGR 309

Query: 353 TRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTAIRPTFGTVGR 412
           TRNPWNIEEF+TGSSAGPAACTSAGMVPFAIGSET GSMTYPAARCGVTAIRPTFGTVGR
Sbjct: 310 TRNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRPTFGTVGR 369

Query: 413 SGVMSISESLKR 424
           SGVMSISESL +
Sbjct: 370 SGVMSISESLDK 374

BLAST of Cucsat.G849.T27 vs. NCBI nr
Match: TYK19429.1 (glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo var. makuwa])

HSP 1 Score: 589 bits (1518), Expect = 1.43e-205
Identity = 290/300 (96.67%), Postives = 295/300 (98.33%), Query Frame = 0

Query: 115 SKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASEN 174
           SKKDSECLENMGRTS+LKCVFRA DADFFNNTKRDEVLKGAEE+NVP IRANRKLVASEN
Sbjct: 15  SKKDSECLENMGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVASEN 74

Query: 175 GGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQ 234
           GGLHNPS LVFNPEWANEN RHKSKRFCYPPV G+KRPNNEDDIAFMSVLELGELIKTEQ
Sbjct: 75  GGLHNPSPLVFNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIKTEQ 134

Query: 235 ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK 294
           ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK
Sbjct: 135 ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK 194

Query: 295 DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR 354
           DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR
Sbjct: 195 DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR 254

Query: 355 NPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTAIRPTFGTVGRSG 414
           NPWNIEEF+TGSSAGPAACTSAGMVPFAIGSET+GSMTYPAARCGVTAIRPTFGTVGRSG
Sbjct: 255 NPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSG 314

BLAST of Cucsat.G849.T27 vs. ExPASy TrEMBL
Match: A0A0A0L5U7 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G039330 PE=4 SV=1)

HSP 1 Score: 734 bits (1895), Expect = 1.91e-261
Identity = 375/403 (93.05%), Postives = 376/403 (93.30%), Query Frame = 0

Query: 22  SAGGDSVVTQQSATPFDKCPTMASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSW 81
           SAGGDSVVTQQSATPFDKCPTMASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSW
Sbjct: 54  SAGGDSVVTQQSATPFDKCPTMASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSW 113

Query: 82  RRFTLMMGTYKKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDAD 141
           RRFTLM                          DSKKDSECLENMGRTSVLKCVFRALDAD
Sbjct: 114 RRFTLM--------------------------DSKKDSECLENMGRTSVLKCVFRALDAD 173

Query: 142 FFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRF 201
           FFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRF
Sbjct: 174 FFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRF 233

Query: 202 CYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSF 261
           CYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSF
Sbjct: 234 CYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSF 293

Query: 262 TEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAW 321
           TEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAW
Sbjct: 294 TEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAW 353

Query: 322 VYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPF 381
           VYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPF
Sbjct: 354 VYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPF 413

Query: 382 AIGSETIGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLKR 424
           AIGSETIGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESL +
Sbjct: 414 AIGSETIGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDK 430

BLAST of Cucsat.G849.T27 vs. ExPASy TrEMBL
Match: A0A1S4DX80 (glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo OX=3656 GN=LOC103490981 PE=4 SV=1)

HSP 1 Score: 660 bits (1702), Expect = 1.65e-233
Identity = 325/337 (96.44%), Postives = 331/337 (98.22%), Query Frame = 0

Query: 88  MGTYKKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTK 147
           MGTYKKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTS+LKCVFRA DADFFNNTK
Sbjct: 1   MGTYKKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTSILKCVFRAFDADFFNNTK 60

Query: 148 RDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVS 207
           RDEVLKGAEE+NVP IRANRKLVASENGGLHNPS LVFNPEWANEN RHKSKRFCYPPV 
Sbjct: 61  RDEVLKGAEEVNVPTIRANRKLVASENGGLHNPSPLVFNPEWANENARHKSKRFCYPPVH 120

Query: 208 GIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAY 267
           G+KRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAY
Sbjct: 121 GVKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAY 180

Query: 268 KQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLK 327
           KQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLK
Sbjct: 181 KQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLK 240

Query: 328 SAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSET 387
           SAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEF+TGSSAGPAACTSAGMVPFAIGSET
Sbjct: 241 SAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSET 300

Query: 388 IGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLKR 424
           +GSMTYPAARCGVTAIRPTFGTVGRSGVMSISESL +
Sbjct: 301 VGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDK 337

BLAST of Cucsat.G849.T27 vs. ExPASy TrEMBL
Match: A0A5D3D785 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00780 PE=4 SV=1)

HSP 1 Score: 589 bits (1518), Expect = 6.94e-206
Identity = 290/300 (96.67%), Postives = 295/300 (98.33%), Query Frame = 0

Query: 115 SKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASEN 174
           SKKDSECLENMGRTS+LKCVFRA DADFFNNTKRDEVLKGAEE+NVP IRANRKLVASEN
Sbjct: 15  SKKDSECLENMGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVASEN 74

Query: 175 GGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQ 234
           GGLHNPS LVFNPEWANEN RHKSKRFCYPPV G+KRPNNEDDIAFMSVLELGELIKTEQ
Sbjct: 75  GGLHNPSPLVFNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIKTEQ 134

Query: 235 ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK 294
           ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK
Sbjct: 135 ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK 194

Query: 295 DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR 354
           DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR
Sbjct: 195 DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR 254

Query: 355 NPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTAIRPTFGTVGRSG 414
           NPWNIEEF+TGSSAGPAACTSAGMVPFAIGSET+GSMTYPAARCGVTAIRPTFGTVGRSG
Sbjct: 255 NPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSG 314

BLAST of Cucsat.G849.T27 vs. ExPASy TrEMBL
Match: A0A6J1G4E4 (uncharacterized protein LOC111450695 OS=Cucurbita moschata OX=3662 GN=LOC111450695 PE=4 SV=1)

HSP 1 Score: 582 bits (1499), Expect = 5.35e-202
Identity = 306/393 (77.86%), Postives = 336/393 (85.50%), Query Frame = 0

Query: 36  PFDKCPTMASTPTI---NCFV-VFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMMGTY 95
           P DK  +M +T T    NCF  VFF FFFF V +RSSIS +FSS  Q S   FT M  TY
Sbjct: 2   PIDKWLSMPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQ-SRPCFTAMGATY 61

Query: 96  KKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEV 155
           KK WKLL EKVGVH    S+     KD+E  +N G+ S     FRA DADFF+NT+ DEV
Sbjct: 62  KKIWKLLCEKVGVHYRSPSY---RNKDTESFKNGGKRSF----FRAFDADFFSNTEMDEV 121

Query: 156 LKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKR 215
           LKGAEELN+P+IRANRKLVASENGGLHNPS LVF+  WANE   HKS+RFCYPPVSG+KR
Sbjct: 122 LKGAEELNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKR 181

Query: 216 PNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAK 275
           P NE+DIAFMSVLELGELIKT+QI+SQELV IFL+RLKRY++VL+AVVSFTE+LAYKQAK
Sbjct: 182 PKNEEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAK 241

Query: 276 EADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGA 335
           EADELFA+GVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVY+QLKSAGA
Sbjct: 242 EADELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGA 301

Query: 336 VLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSM 395
           VLV KLVTGSLAYDDIWFGGRTRNPWNIEEF+TGSSAGPAACTSAG+VPFAIGSET GSM
Sbjct: 302 VLVTKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSM 361

Query: 396 TYPAARCGVTAIRPTFGTVGRSGVMSISESLKR 424
           TYPA+RCGVTA+RPTFGTVGRSGVMSISESL +
Sbjct: 362 TYPASRCGVTALRPTFGTVGRSGVMSISESLDK 386

BLAST of Cucsat.G849.T27 vs. ExPASy TrEMBL
Match: A0A6J1KYV3 (uncharacterized protein LOC111498360 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498360 PE=4 SV=1)

HSP 1 Score: 573 bits (1476), Expect = 1.69e-198
Identity = 303/393 (77.10%), Postives = 332/393 (84.48%), Query Frame = 0

Query: 36  PFDKCPTMASTPTI---NCFV-VFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMMGTY 95
           P DK  +M +T T    NCF  VFF F FF V +RSSIS +FSS  Q S   FT M  TY
Sbjct: 2   PIDKWLSMPATRTAIASNCFFFVFFVFIFFAVPLRSSISPNFSSSDQ-SRPCFTAMGATY 61

Query: 96  KKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEV 155
           KK WKLL EKVG H  Y S      KD+E  +N G+ S     FRA DA+FF+ T+ DEV
Sbjct: 62  KKIWKLLCEKVGDH--YRSPPSYRNKDTESFKNGGKRSF----FRAFDAEFFSKTEMDEV 121

Query: 156 LKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKR 215
           LKGAEE N P+IRANRKLVASENGGLHNPS LVF+  WANE  +HKS+RFCYPPV G+KR
Sbjct: 122 LKGAEEFNFPVIRANRKLVASENGGLHNPSPLVFSSNWANEGFQHKSRRFCYPPVFGVKR 181

Query: 216 PNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAK 275
           P NE+DIAFMSVLELGELIKT+QI+SQELV IFLQRLKRY++VL+AVVSFTE+LAYKQAK
Sbjct: 182 PKNEEDIAFMSVLELGELIKTKQITSQELVGIFLQRLKRYDYVLQAVVSFTEDLAYKQAK 241

Query: 276 EADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGA 335
           EADELFA+GVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVY+QLKSAGA
Sbjct: 242 EADELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGA 301

Query: 336 VLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSM 395
           VLVAKLVTGSLAYDDIWFGGRTRNPWNIEEF+TGSSAGPAACTSAG+VPFAIGSET GSM
Sbjct: 302 VLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSM 361

Query: 396 TYPAARCGVTAIRPTFGTVGRSGVMSISESLKR 424
           TYPA+RCGVTA+RPTFGTVGRSGVMSISESL +
Sbjct: 362 TYPASRCGVTALRPTFGTVGRSGVMSISESLDK 387

BLAST of Cucsat.G849.T27 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 479.6 bits (1233), Expect = 2.7e-135
Identity = 238/338 (70.41%), Postives = 278/338 (82.25%), Query Frame = 0

Query: 87  MMGTYKKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNT 146
           M+   K  WKLL  KV V     + L  +   +E  +       L+ +F  +D+ FFN T
Sbjct: 69  MVRNCKNLWKLLDRKVSVPCRKITTLTRTVTLTELPDKTMEAKCLQGMFELIDSAFFNET 128

Query: 147 KRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPV 206
           K  E+ KGA E+NVPI RANRKLVA++NGGL NPS LVFNP W  E  R + KRF YP  
Sbjct: 129 KLQEIAKGATEMNVPIYRANRKLVATKNGGLENPSPLVFNPSWNREVQRVQDKRFKYPSA 188

Query: 207 SGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELA 266
           SG+K P +E+DIAFMSVLELGELIKT QI+S+ELVRI+L++LKRYNHVLEAVV++TEELA
Sbjct: 189 SGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELA 248

Query: 267 YKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQL 326
           YKQAKEAD+L +QG YLGPLHGIPYGLKDI++VPGYKTTWGS SFK+Q +D+EAWVYK+L
Sbjct: 249 YKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAVPGYKTTWGSTSFKDQFLDIEAWVYKRL 308

Query: 327 KSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSE 386
           K++GAVLVAKLVTGS+AYDDIWFGGRTRNPWNIEEFSTGSSAGPAA TSAGMVPFAIGSE
Sbjct: 309 KASGAVLVAKLVTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSE 368

Query: 387 TIGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLKR 425
           T GSMTYPAARCG+TA+RPTFG+VGR+GVMSISESL +
Sbjct: 369 TAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDK 406

BLAST of Cucsat.G849.T27 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 459.1 bits (1180), Expect = 3.7e-129
Identity = 231/338 (68.34%), Postives = 271/338 (80.18%), Query Frame = 0

Query: 87  MMGTYKKFWKLLYEKVGVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNT 146
           M+   K  WKLL  KV V     + L  +   +E  +       L+ +F  +D+ FFN T
Sbjct: 69  MVRNCKNLWKLLDRKVSVPCRKITTLTRTVTLTELPDKTMEAKCLQGMFELIDSAFFNET 128

Query: 147 KRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPV 206
           K  E+ KGA E+NVPI RANRKLVA++NGGL NPS LVFNP W  E  R + KRF YP  
Sbjct: 129 KLQEIAKGATEMNVPIYRANRKLVATKNGGLENPSPLVFNPSWNREVQRVQDKRFKYPSA 188

Query: 207 SGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELA 266
           SG+K P +E+DIAFMSVLELGELIKT QI+S+ELVRI+L++LKRYNHVLEAVV++TEELA
Sbjct: 189 SGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELA 248

Query: 267 YKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQL 326
           YKQAKEAD+L +QG YLGPLHGIPYGLKDI++VPGYKTTWGS SFK+Q +D+EAWVYK+L
Sbjct: 249 YKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAVPGYKTTWGSTSFKDQFLDIEAWVYKRL 308

Query: 327 KSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSE 386
           K++GAVLVAKLVTGS+AYDDIWFGGRTRNPWNIEEFSTGSSAGPAA TSA       GSE
Sbjct: 309 KASGAVLVAKLVTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSA-------GSE 368

Query: 387 TIGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLKR 425
           T GSMTYPAARCG+TA+RPTFG+VGR+GVMSISESL +
Sbjct: 369 TAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDK 399

BLAST of Cucsat.G849.T27 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 98.2 bits (243), Expect = 1.7e-20
Identity = 61/197 (30.96%), Postives = 101/197 (51.27%), Query Frame = 0

Query: 234 QISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGL 293
           + ++ E+ + +L R++     L+  +  +E +  K A+E D+  A+G  LGPL G+  G+
Sbjct: 57  ETTAVEIAKSYLSRIRLTEPQLKCFLHVSENV-LKDAQEIDQRIAKGEELGPLAGVLIGV 116

Query: 294 KDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRT 353
           KD I   G  +T  S+  +      +A   K++K  G ++V     G    D+   G  T
Sbjct: 117 KDNICTQGMPSTAASRILEHYRPPFDATAVKKIKELGGIVV-----GKTNMDEFGMGSTT 176

Query: 354 R--------NPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTAIRP 413
                    NPW++     GSS G AA  +A     ++GS+T GS+  PA+ CGV  ++P
Sbjct: 177 EASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKP 236

Query: 414 TFGTVGRSGVMSISESL 423
           T+G V R G+M+ + SL
Sbjct: 237 TYGRVSRFGLMAYASSL 247

BLAST of Cucsat.G849.T27 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 68.9 bits (167), Expect = 1.1e-11
Identity = 44/141 (31.21%), Postives = 69/141 (48.94%), Query Frame = 0

Query: 286 LHGIPYGLKDIISVPGYKTTWGSKSF--KEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLA 345
           L G+ + +KDI  V G  T +G+  +          A V   L  AGA  +   +   +A
Sbjct: 28  LQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPVVSSLLEAGATALGITIMDEMA 87

Query: 346 YD---DIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGV 405
           Y    +    G  RNP   +    GSS+G A   +A +V F+IG++T GS+  PA+ CG+
Sbjct: 88  YSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLVDFSIGTDTGGSVRVPASYCGI 147

Query: 406 TAIRPTFGTVGRSGVMSISES 422
              RP+ G V   G+  +++S
Sbjct: 148 FGFRPSHGAVSTVGLTPMAQS 168

BLAST of Cucsat.G849.T27 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 66.6 bits (161), Expect = 5.4e-11
Identity = 45/143 (31.47%), Postives = 67/143 (46.85%), Query Frame = 0

Query: 286 LHGIPYGLKDIISVPGYKTTWGSKSFKE--QVIDVEAWVYKQLKSAGAVLVAKLVTGSLA 345
           L G+ + + D   V  Y T +G   +K+  +  +  A V   L   GA  V K +   L 
Sbjct: 71  LSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAEKTAVVVTTLLKNGATCVGKTIMDELG 130

Query: 346 YDDIWFG---GRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGV 405
           +  I      G   NP   +    G S+G A    A +V F++G +T G +  PAA CG+
Sbjct: 131 FGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSVGAELVDFSLGIDTTGGVRVPAAFCGI 190

Query: 406 TAIRPTFGTVGRSGVMSISESLK 424
              RP+ GTV   GV+  S+SL+
Sbjct: 191 LGFRPSQGTVSSVGVLPNSQSLE 213

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3AD365.4e-3237.32Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Carboxydothermus hydrogenoforma... [more]
B6J4H51.3e-3039.22Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain CbuK_... [more]
B6IZ251.3e-3039.22Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain CbuG_... [more]
A9KBI01.3e-3039.22Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain Dugwa... [more]
A9N8Z41.3e-3039.22Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain RSA 3... [more]
Match NameE-valueIdentityDescription
XP_011650445.13.31e-27299.48uncharacterized protein LOC101214338 isoform X1 [Cucumis sativus] >KAE8650086.1 ... [more]
XP_011650446.12.14e-24592.67uncharacterized protein LOC101214338 isoform X2 [Cucumis sativus][more]
XP_016900589.13.40e-23396.44PREDICTED: glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo][more]
XP_038905194.12.34e-21585.22glutamyl-tRNA(Gln) amidotransferase subunit A [Benincasa hispida][more]
TYK19429.11.43e-20596.67glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A0A0L5U71.91e-26193.05Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G039330 PE=... [more]
A0A1S4DX801.65e-23396.44glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5D3D7856.94e-20696.67Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1G4E45.35e-20277.86uncharacterized protein LOC111450695 OS=Cucurbita moschata OX=3662 GN=LOC1114506... [more]
A0A6J1KYV31.69e-19877.10uncharacterized protein LOC111498360 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G07360.12.7e-13570.41Amidase family protein [more]
AT5G07360.23.7e-12968.34Amidase family protein [more]
AT3G25660.11.7e-2030.96Amidase family protein [more]
AT1G08980.11.1e-1131.21amidase 1 [more]
AT5G09420.15.4e-1131.47translocon at the outer membrane of chloroplasts 64-V [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 188..424
e-value: 3.1E-67
score: 229.7
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 206..421
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 239..422
e-value: 7.5E-46
score: 157.1
NoneNo IPR availablePANTHERPTHR11895:SF73AMIDASE FAMILY PROTEINcoord: 39..423
IPR000120AmidasePANTHERPTHR11895TRANSAMIDASEcoord: 39..423

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G849Cucsat.G849gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G849.T27.E1Cucsat.G849.T27.E1exon
Cucsat.G849.T27.E2Cucsat.G849.T27.E2exon
Cucsat.G849.T27.E3Cucsat.G849.T27.E3exon
Cucsat.G849.T27.E4Cucsat.G849.T27.E4exon
Cucsat.G849.T27.E5Cucsat.G849.T27.E5exon
Cucsat.G849.T27.E6Cucsat.G849.T27.E6exon
Cucsat.G849.T27.E7Cucsat.G849.T27.E7exon
Cucsat.G849.T27.E8Cucsat.G849.T27.E8exon
Cucsat.G849.T27.E9Cucsat.G849.T27.E9exon
Cucsat.G849.T27.E10Cucsat.G849.T27.E10exon
Cucsat.G849.T27.E11Cucsat.G849.T27.E11exon
Cucsat.G849.T27.E12Cucsat.G849.T27.E12exon
Cucsat.G849.T27.E13Cucsat.G849.T27.E13exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G849.T27.C1Cucsat.G849.T27.C1CDS
Cucsat.G849.T27.C2Cucsat.G849.T27.C2CDS
Cucsat.G849.T27.C3Cucsat.G849.T27.C3CDS
Cucsat.G849.T27.C4Cucsat.G849.T27.C4CDS
Cucsat.G849.T27.C5Cucsat.G849.T27.C5CDS
Cucsat.G849.T27.C6Cucsat.G849.T27.C6CDS
Cucsat.G849.T27.C7Cucsat.G849.T27.C7CDS
Cucsat.G849.T27.C8Cucsat.G849.T27.C8CDS
Cucsat.G849.T27.C9Cucsat.G849.T27.C9CDS
Cucsat.G849.T27.C10Cucsat.G849.T27.C10CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G849.T27Cucsat.G849.T27-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016740 transferase activity