Cucsat.G849.T25 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G849.T25
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	glutamyl-tRNA(Gln) amidotransferase subunit A
Location	ctg1: 2907079 .. 2912979 (+)
RNA-Seq Expression	Cucsat.G849.T25
Synteny	Cucsat.G849.T25

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTCCATCAAAATAATCTCATTTTTTTTTGGCAATTTCCCATCCCAATTCAGTGCCGGCGGTGACTCAGTGGTCACTCAGCAGTCAGCCACGCCGTTCGACAAGTGTCCTACAATGGCGTCGACTCCTACTATTAACTGCTTCGTTGTCTTCTTCTTCTTCTTCTTCTTCACAGTTTCGGTTCGATCTTCCATTTCCTCCCACTTCTCTTCTTTCCCTCAATGTTCTTGGCGTCGCTTCACACTAATGGTAACTTCTCTTCTGCTTTTACATCGCAATTTTCTCCCTATTATGCTTTTTTGTTCTTCTTTTGTTATCGGAAGGAATCATCACTTCAACGTGTTCTGGAAAAGCCCCAATTAGATACTATACCCTGCTTGTTTAAATCTTAGATTATGATTTTCCTATCCACATATTGTATAAAATAAATTACTTTTTGTTTTTCATTAGAGATGAAGAGAGGGGATGGAGGCTAGAGCTAAGAGGATATGAAGGGTTAATAATAATAACATTAATGATACTTGTATATTAATATATGATAATAATTTCAAATTGGTTGCCAAGAAGAGTATGACTTCACCAATTTAATTTAGTTTTTCTTTTTACAATTTAGGGGTAAGGGATTGGACCTCCAAGTTATATGTCATTCTTGATATTCTTATTAAGCTTTGCTATTTTTCCCACCAATTTTGTATTATTATCATATATTGCATTGCAATTATTATTAATGTTTATTATTGTTTGGGTTAGTTGCTTAAATGACATTTAAGCATAACTCAATAAATAAGTAAAATTAGAATATTTAGCCCAAAGATAAAATAATTACATATATAGCTCAAAAGAAAAAAAGTTGGGAAAAAAACTCACACCTATAAATGTTATCACTTATAGCTACTATCTAACGGTAACTTTAAATTTGAGAAATGTGTTCATGAATGGATATCAACTAAGAAGCACAAACACCGATAGGTCGATTTCTAAAATTTTAGGATACTGACACGTGTAAGCACAATGAATTGCTATCGTTGACAGCTATCAGGGATGGAAACATGTCCATTGATAGCTATTAGCAATGAAAACATGTGCATTGAAGGGTACACCTAGAATTCTAAAAATTTTACAAGTTGATTGGTATTTGTTATACTTACAACTATTTCAAAGTTACGTTATATTTTTATACTATTTTCCTAATTGTGTTATTTGCTATAATTTTCTTTATTGTTATGGAGCATTTTCCAATATAGCAAAAACTTGGTTAAATTTCTCACTCAGAACTTGTCATCTGACATGTTATTTTCCTAAACCTGGGGATAATTGTGAGACCATTTAAAAGTATTTTACAAATCTCAGGAATTGAAGGACAACTTTTAGAACTTCGGATTAGCTCGTTTACACGGTTGGAAATTTAGAAACTTAGCTCTTGCTATAACTTTCAAAAGTAAATTGATAGATGTCGGTTGAGGCTCTTGGTTGCATTGAGGTGGGCCAAATTTTACTCGATTCTTTATTTATAGAGAAAGCCAAAATTTACCATAGAGAGTAATTTTGTAATTCCTAATCTAGGTCCCAAATGAAAGGACCAAACTCACAATGTTCTGTTTTTTTATTCAAGTGCAAAAACTATCACAAACACTAATTATAATAAGTGTTTAGAAGTTTGATATTCACTAATATATTAAGCTCTCAGTCTTACAGGAATATCGATGTAAATTAAAATGTCGATATCAATATTTTGTAAAATAATAATAAAAAGTAAAAAGATTGTTTAAATGAATCAGTAAACATTTCTCATTCATTAATAAAATGCTTGTTATTCGTATTATATGCTATTTATATTTGCTACAATTTGTTGTTTGTGTTTCATCAGACCATATAATAAAAATGTTGACATGTGGACAAAAATTTAGTACTCTACTTCCAGGGCATTTTGAAGTCTTTTTTTGTATCTTTTTTTTAAAAACTATTCTTTCTTGATAAATGTTAAACAGATGGGAACTTATAAAAAATTCTGGAAACTTTTGTATGAAAAAGTTGGGGTTCATAAGCCATATTCTTCCTTTCTGCAGGTAACATTTGTTAATTACTCTGCTAACCAATTGTTCTACTCCATTTTCGTCTTGTTGTTTCTTTTCATGTTCTTTTCATCAGGACAGCAAGAAAGACTCTGAGTGTTTGGAAAATATGGGGAGAACATCCGTTCTGAAATGTGTATTTAGAGCACTTGATGCAGATTTCTTCAATAACACCAAGGTTCAACAAAGCACCAATTGTTTTCATGTAGTTATATATTGTGTTGTCTTTTACACTCATGTAACGTTGAGAAACTTTTGTTTTTGAGTAGAGGGATGAGGTTTTAAAGGGAGCTGAAGAACTTAATGTGCCTATAATCAGAGCCAACCGGAAGTTAGTTGCTTCTGAAAATGGAGGTCTGCATAATCCTTCCTCTTTGGTGTTCAACCCTGAGTGGGCAAATGAAAACGTTCGGCATAAGAGCAAAAGATTCTGTTATCCTCCTGTTTCTGGAATAAAGAGACCAAACAACGAAGACGATATTGCTTTCATGAGTGTACGTCTGCACTGATCTTTTTAGTTTGAATTGAAGCTAAATATGTACTTTAGTCTCGAGTGTTTGAGTTTTTTTTTTTCCTTTTTCCCTCTGATTTGGAAAAAAGGCCCACAGTTACAAATGTTGGAATTTCGTTGATCGAGTAAAAAATATGTTTTGCTTGATGTAACCATAAAATAAAATAGTATTCATTTTATATCCCTTAGCAAGTAGTAACTTGTTTGGCCCAAAATTGCTTATCGTTCTCTAAAATCCATCCATCCAAATATTGGAATACTATCAAATTAAAACCTAATTGTTTTTAGATTCATATGATCCTTCTTTTAACATTAGGGACTAAAGGGAGTGATGGTTGAATTCTATTAGGTTCTTGAACTTGGAGAACTGATTAAAACGGAGCAGATTTCATCTCAGGAGCTTGTTCGAATTTTTCTTCAGAGATTGAAAAGGTATATAAAAAACCAACTTACAGTCTTTCGGATCGAAGTTGAAGTTCTTATTTAAAAATTTTCTTTAAACAATATCAGGTACAACCATGTTCTTGAAGCTGTAGTTTCTTTTACTGAAGAACTGGCTTACAAACAAGCAAAAGAGGCTGATGAACTGTTTGCTCAAGGAGTGTATTTAGGTTCGTGTCTTTTATGATGATAATTTTTTGCCCTACTTCAATGAAAGTCGTTGATTTGGGTGATGCATTTATGAATTGTAGTTCACTGTTTGACTTTCGACTTTATTTTTCAAATGCGGTGTTAAGTTTATACTTCACTTTGAAATAAAATATTATTCTTGCAGAGTTAAATTTATACCTTTAAACTTAGAAACAAAAAGTTCTTGTTTTGCAGGTCCACTGCATGGAATTCCATATGGGTTGAAGGATATAATATCTGTTCCAGGATACAAAACAACTTGGGGTTCAAAAAGTTTTAAAGAACAAGTAATTGATGTTGAAGCTTGGGTTTATAAGCAGTACGAAAAAAATGACTAATTTATCTAAGTATATTAATTGACATCTTTGCCAATAAATCATGAATCATTTGGTTTTTAACATCTTTACAAACAAACCGCGAATCCTTTGGTTTTGCAGGTTGAAGTCAGCAGGTGCTGTTCTTGTTGCAAAACTTGTTACCGGATCACTTGCATATGATGACATCTGGTTTGGTGGTCGTACGAGGAACCCGTGGAACATTGAGGAGTTTAGTACAGGGTCATCAGCTGGACCTGCAGCATGTACTTCTGCAGGTACGATTTAGACTTGGAGCTGATGGTTTCACCACAAAATGTTTTATTTTTCTTAGCAAGTCTTAGAACATCTCCATTACAACATCATCTTGAGTATAATATTTTTGGTTGAATTCCAGCAATTTTCATTTCAACTTGAGACTTCATTGCCTTAACCATTGATTTGCTTCAGGTGTTAGGTTCTTGTGTTTCTTAAGAAAAAAAGAGCTTGTCAAGTTTGTCATAGTTTCTTGCTATAAGAATATTTCCTTCACAATTTTGGATAATGAACCCAGAGGAGATGGGGCTTTATATAATGCATTGCCCCCTTGTTTACTAGGTATGGTTCCATTTGCAATTGGATCAGAGACAATTGGTTCGATGACTTATCCTGCAGCTCGATGTGGAGTTACTGCAATCCGGCCAACATTTGGAACAGTTGGTCGCAGTGGAGTGATGAGTATTTCTGAAAGCTTGGTAGTAGCCACAAGCCCTTTTTGCTTTGTTTCTCAACTCGCCTTTTGTATTTATTCTGCTTTTATTTAACAGGATAAGCTTGGTCCATTTTGTAGAAACGCGGTTGATTGTGCAGTCGTTTTGGATGTCATTAGAGGGAAGGATCCACATGACCTCTCCTCAGTTGAGAGTTCTCTTGATGATCCATTCTCCATTGACATTTCTAAACTTACTGTTGGATATCTTGACGATGCTGACATGGAGGTCAGTAACTCCATCAGAGTAGAGCCTTCTTCTTTGACATCTTTGATGTTAACCCAACTTTTAAAATGTTTATTATAGGTTGTTCGGATTCTTTCCTCTAAGGGTGTCAATATGGTTCCATTCAACTTGTCTTATTCTGTTGATTCTGTTCAAGGCATTATTAACTTCACTATGGATGTTGATATGTTGGCTCATTTTGACGAATGGCAACGTTTGGGGCTTGATGATGAATATGAAGCTCAAGACCAATGGCCAACTGAACTCCGTCGTGCACGTTTAATCCCAGCCGTAGATTATGTTCAGGTATTGTCTACATAAAGACTCAGTCTTCATTTATCAATAAACTTGATCCCGATTAGTTCATTAGTTTACACGAGCAATAAAATTCATTAAAAACTTTACTATACTACTTTTTTCCATAGACGGAAATTGTTTGTTGCACATTCTAATGTAAGACTAAATTAATACTTATTGAAGTTCAGGAAAAACGTCGGACGAGTATAGGAAGTTAATAGTTACCAACCAGACCGTTGTGACCAATTTTTTTTTTTTAACCCTTGATTATCCAATCCATGCACAGGCACAGAGAGCAAGGGGAAAGCTAATTAGAGAAGTTAGAGAAAGTTTCAACGTTGATGCTTTAATTGGCAATGCCACTGACTGGGAGAAAGTTTGTATGGGAAATCTTGTTGGTCTGCCTATTGTTGTTGTTCCAACAGGTTTCAAAAACATCTCAAACCCACCGTCAACTGGCACCACAAGAAGAAAAACCACTATTACCACTGGCATTTATGCTCCTCCTCACAGAGATCACATTGTATGCAACTTTTTCTTTTCTTGGAATAAAAAAAATAAGACTCCTTTTGGTTTTTGTGGGTAACTACTTATTGTATTTGATGAATGATTGTTTGTGTTGTAGGCTTTAGCTTTGGCTATGGCTTACCAATCTGCAACTGATCACCACAGAGCAAAGCCACCAATAGATGACTTGGGGCCCAGTGACGTTCTTCCAGATCCACCTTTGGTATCTATTCCTCCAAGGTTATTGCACCTTTGAACATGAATCTGCACCTCTTTTCCCTTGTCTTATCTTACATTTTGGAGACTTTTGTCTTCTGTTTTTTCTTTTTGGCTATAGAGAAAAACAGAAATATAGTTTAGTCCCGAATGTTAATATGCATACATCTACGACTGCGACATTCTCGATGTCAGAGTTTTTGTATGTAAATGCTGCTTCTAGTTCAAGCTCTATCATCCATTAAGTAATATAATCAATGTTATGGAATTAAAACTAGTTCATATTTTCACGTCTTTCCCTTTTTATTTCTGAAAATTGTTCCAAAGCCACCTCTGTTTACAGTTTCTACGATTGTTGCTGTTGAGAGATTTGGGGTTC

Coding sequence (CDS)

ATGGGGAGAACATCCGTTCTGAAATGTGTATTTAGAGCACTTGATGCAGATTTCTTCAATAACACCAAGAGGGATGAGGTTTTAAAGGGAGCTGAAGAACTTAATGTGCCTATAATCAGAGCCAACCGGAAGTTAGTTGCTTCTGAAAATGGAGGTCTGCATAATCCTTCCTCTTTGGTGTTCAACCCTGAGTGGGCAAATGAAAACGTTCGGCATAAGAGCAAAAGATTCTGTTATCCTCCTGTTTCTGGAATAAAGAGACCAAACAACGAAGACGATATTGCTTTCATGAGTGTTCTTGAACTTGGAGAACTGATTAAAACGGAGCAGATTTCATCTCAGGAGCTTGTTCGAATTTTTCTTCAGAGATTGAAAAGGTACAACCATGTTCTTGAAGCTGTAGTTTCTTTTACTGAAGAACTGGCTTACAAACAAGCAAAAGAGGCTGATGAACTGTTTGCTCAAGGAGTGTATTTAGGTTGA

Protein sequence

MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG

Homology

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BLAST of Cucsat.G849.T25 vs. ExPASy Swiss-Prot
Match: Q8RC40 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=gatA PE=3 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 6.2e-05
Identity = 23/60 (38.33%), Postives = 41/60 (68.33%), Query Frame = 0


Query: 97  MSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQG 156
           ++V EL EL+   ++S+QE+ + +L+R+K     ++A+V+ TEE A ++AK ADE+   G
Sbjct: 6   LTVHELKELLHKREVSAQEVTKSYLERIKEVEPKIDALVTITEEFALERAKVADEMIKNG 65

[Close]

BLAST of Cucsat.G849.T25 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 8.1e-05
Identity = 22/60 (36.67%), Postives = 42/60 (70.00%), Query Frame = 0


Query: 97  MSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQG 156
           +++ EL EL+K  ++S+ E+ + +L+R+K     ++A+V+ TE+ A ++AKEADE   +G
Sbjct: 6   LTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIKKG 65

[Close]

BLAST of Cucsat.G849.T25 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.4e-04
Identity = 21/60 (35.00%), Postives = 41/60 (68.33%), Query Frame = 0


Query: 97  MSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQG 156
           +++ EL EL+K  ++S+ E+ + +L+R+K     ++A+++ TE+ A ++AKEADE    G
Sbjct: 6   LTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQRAKEADEKIKNG 65

[Close]

BLAST of Cucsat.G849.T25 vs. ExPASy Swiss-Prot
Match: Q31F53 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Hydrogenovibrio crunogenus (strain DSM 25203 / XCL-2) OX=317025 GN=gatA PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.8e-04
Identity = 23/60 (38.33%), Postives = 41/60 (68.33%), Query Frame = 0


Query: 97  MSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQG 156
           +SV E+ E ++ ++I+S  L + +L R+ +Y+  L A V+ T ELA + A++ADE+ A+G
Sbjct: 4   LSVKEMSEKLRNKEITSVALTQHYLDRIDQYDGELNAYVTVTPELAMEMARQADEMIAEG 63

[Close]

BLAST of Cucsat.G849.T25 vs. ExPASy Swiss-Prot
Match: B6J4H5 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain CbuK_Q154) OX=434924 GN=gatA PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.9e-04
Identity = 25/59 (42.37%), Postives = 36/59 (61.02%), Query Frame = 0


Query: 98  SVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQG 157
           ++ EL + ++ + ISS EL + FL R+K  N  L + +S TEE A  QAK AD   A+G
Sbjct: 5   TIAELKQDLRDKTISSVELTQHFLDRIKTINPTLNSFISITEEYALTQAKAADARLAKG 63

[Close]

BLAST of Cucsat.G849.T25 vs. NCBI nr
Match: XP_011650446.1 (uncharacterized protein LOC101214338 isoform X2 [Cucumis sativus])

HSP 1 Score: 317 bits (813), Expect = 5.35e-103
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 60
           MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV
Sbjct: 57  MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 116

Query: 61  FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 120
           FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF
Sbjct: 117 FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 176

Query: 121 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 160
           LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG
Sbjct: 177 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 216

[Close]

BLAST of Cucsat.G849.T25 vs. NCBI nr
Match: XP_011650445.1 (uncharacterized protein LOC101214338 isoform X1 [Cucumis sativus] >KAE8650086.1 hypothetical protein Csa_011729 [Cucumis sativus])

HSP 1 Score: 317 bits (813), Expect = 1.05e-102
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 60
           MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV
Sbjct: 83  MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 142

Query: 61  FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 120
           FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF
Sbjct: 143 FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 202

Query: 121 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 160
           LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG
Sbjct: 203 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 242

[Close]

BLAST of Cucsat.G849.T25 vs. NCBI nr
Match: TYK19429.1 (glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo var. makuwa])

HSP 1 Score: 306 bits (785), Expect = 3.12e-99
Identity = 152/160 (95.00%), Postives = 155/160 (96.88%), Query Frame = 0


Query: 1   MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 60
           MGRTS+LKCVFRA DADFFNNTKRDEVLKGAEE+NVP IRANRKLVASENGGLHNPS LV
Sbjct: 25  MGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVASENGGLHNPSPLV 84

Query: 61  FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 120
           FNPEWANEN RHKSKRFCYPPV G+KRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF
Sbjct: 85  FNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 144

Query: 121 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 160
           LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG
Sbjct: 145 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 184

[Close]

BLAST of Cucsat.G849.T25 vs. NCBI nr
Match: XP_016900589.1 (PREDICTED: glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo])

HSP 1 Score: 306 bits (785), Expect = 5.45e-99
Identity = 152/160 (95.00%), Postives = 155/160 (96.88%), Query Frame = 0


Query: 1   MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 60
           MGRTS+LKCVFRA DADFFNNTKRDEVLKGAEE+NVP IRANRKLVASENGGLHNPS LV
Sbjct: 38  MGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVASENGGLHNPSPLV 97

Query: 61  FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 120
           FNPEWANEN RHKSKRFCYPPV G+KRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF
Sbjct: 98  FNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 157

Query: 121 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 160
           LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG
Sbjct: 158 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 197

[Close]

BLAST of Cucsat.G849.T25 vs. NCBI nr
Match: XP_038905194.1 (glutamyl-tRNA(Gln) amidotransferase subunit A [Benincasa hispida])

HSP 1 Score: 287 bits (735), Expect = 4.01e-91
Identity = 141/160 (88.12%), Postives = 151/160 (94.38%), Query Frame = 0


Query: 1   MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 60
           +G+TS+LKCVFRA DADFFNNTK DEVLKGAEE N+P IRANRKLVAS NGGLHNPS LV
Sbjct: 75  VGKTSLLKCVFRAFDADFFNNTKMDEVLKGAEEFNLPTIRANRKLVASRNGGLHNPSPLV 134

Query: 61  FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 120
           FNPEWANEN+RHKSKRFCYP VSG+KRP NE+DIAFMSVLELGELIKT+QISSQELVRIF
Sbjct: 135 FNPEWANENIRHKSKRFCYPSVSGVKRPKNEEDIAFMSVLELGELIKTKQISSQELVRIF 194

Query: 121 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 160
           LQRLKRYNHVL+AVVSFTEELAYKQAKEADELFA+G YLG
Sbjct: 195 LQRLKRYNHVLKAVVSFTEELAYKQAKEADELFARGTYLG 234

[Close]

BLAST of Cucsat.G849.T25 vs. ExPASy TrEMBL
Match: A0A0A0L5U7 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G039330 PE=4 SV=1)

HSP 1 Score: 317 bits (813), Expect = 1.69e-102
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 60
           MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV
Sbjct: 131 MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 190

Query: 61  FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 120
           FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF
Sbjct: 191 FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 250

Query: 121 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 160
           LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG
Sbjct: 251 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 290

[Close]

BLAST of Cucsat.G849.T25 vs. ExPASy TrEMBL
Match: A0A5D3D785 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00780 PE=4 SV=1)

HSP 1 Score: 306 bits (785), Expect = 1.51e-99
Identity = 152/160 (95.00%), Postives = 155/160 (96.88%), Query Frame = 0


Query: 1   MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 60
           MGRTS+LKCVFRA DADFFNNTKRDEVLKGAEE+NVP IRANRKLVASENGGLHNPS LV
Sbjct: 25  MGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVASENGGLHNPSPLV 84

Query: 61  FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 120
           FNPEWANEN RHKSKRFCYPPV G+KRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF
Sbjct: 85  FNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 144

Query: 121 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 160
           LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG
Sbjct: 145 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 184

[Close]

BLAST of Cucsat.G849.T25 vs. ExPASy TrEMBL
Match: A0A1S4DX80 (glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo OX=3656 GN=LOC103490981 PE=4 SV=1)

HSP 1 Score: 306 bits (785), Expect = 2.64e-99
Identity = 152/160 (95.00%), Postives = 155/160 (96.88%), Query Frame = 0


Query: 1   MGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLV 60
           MGRTS+LKCVFRA DADFFNNTKRDEVLKGAEE+NVP IRANRKLVASENGGLHNPS LV
Sbjct: 38  MGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVASENGGLHNPSPLV 97

Query: 61  FNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 120
           FNPEWANEN RHKSKRFCYPPV G+KRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF
Sbjct: 98  FNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIF 157

Query: 121 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 160
           LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG
Sbjct: 158 LQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 197

[Close]

BLAST of Cucsat.G849.T25 vs. ExPASy TrEMBL
Match: A0A6J1CPF2 (uncharacterized protein LOC111013354 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013354 PE=4 SV=1)

HSP 1 Score: 259 bits (661), Expect = 1.77e-80
Identity = 126/159 (79.25%), Postives = 144/159 (90.57%), Query Frame = 0


Query: 2   GRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVF 61
           G+ S+LK VFRA DADFFNNTK +E+L+G EE NVPI RANRKLVASENGGLHNPS LVF
Sbjct: 71  GKGSLLKHVFRAFDADFFNNTKMEEILRGEEEFNVPITRANRKLVASENGGLHNPSPLVF 130

Query: 62  NPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFL 121
            P WA+E  RH++ RFCYPPVSG+KRP N++DIAFMSVLELGEL+KT+Q++S ELV+IFL
Sbjct: 131 KPGWADERFRHRNGRFCYPPVSGVKRPKNDEDIAFMSVLELGELVKTKQVTSLELVQIFL 190

Query: 122 QRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 160
           QRLKRYNHVLEAVVSFTEELA+KQAKEADELFA+GVYLG
Sbjct: 191 QRLKRYNHVLEAVVSFTEELAHKQAKEADELFARGVYLG 229

[Close]

BLAST of Cucsat.G849.T25 vs. ExPASy TrEMBL
Match: A0A6J1G4E4 (uncharacterized protein LOC111450695 OS=Cucurbita moschata OX=3662 GN=LOC111450695 PE=4 SV=1)

HSP 1 Score: 253 bits (645), Expect = 6.19e-78
Identity = 124/150 (82.67%), Postives = 140/150 (93.33%), Query Frame = 0


Query: 11  FRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENV 70
           FRA DADFF+NT+ DEVLKGAEELN+P+IRANRKLVASENGGLHNPS LVF+  WANE  
Sbjct: 97  FRAFDADFFSNTEMDEVLKGAEELNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERF 156

Query: 71  RHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHV 130
            HKS+RFCYPPVSG+KRP NE+DIAFMSVLELGELIKT+QI+SQELV IFL+RLKRY++V
Sbjct: 157 LHKSRRFCYPPVSGVKRPKNEEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYV 216

Query: 131 LEAVVSFTEELAYKQAKEADELFAQGVYLG 160
           L+AVVSFTE+LAYKQAKEADELFA+GVYLG
Sbjct: 217 LQAVVSFTEDLAYKQAKEADELFARGVYLG 246

[Close]

BLAST of Cucsat.G849.T25 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 215.3 bits (547), Expect = 3.5e-56
Identity = 108/161 (67.08%), Postives = 129/161 (80.12%), Query Frame = 0


Query: 3   RTSVLKC---VFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSL 62
           +T   KC   +F  +D+ FFN TK  E+ KGA E+NVPI RANRKLVA++NGGL NPS L
Sbjct: 106 KTMEAKCLQGMFELIDSAFFNETKLQEIAKGATEMNVPIYRANRKLVATKNGGLENPSPL 165

Query: 63  VFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRI 122
           VFNP W  E  R + KRF YP  SG+K P +E+DIAFMSVLELGELIKT QI+S+ELVRI
Sbjct: 166 VFNPSWNREVQRVQDKRFKYPSASGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRI 225

Query: 123 FLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 161
           +L++LKRYNHVLEAVV++TEELAYKQAKEAD+L +QG YLG
Sbjct: 226 YLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLG 266

[Close]

BLAST of Cucsat.G849.T25 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 215.3 bits (547), Expect = 3.5e-56
Identity = 108/161 (67.08%), Postives = 129/161 (80.12%), Query Frame = 0


Query: 3   RTSVLKC---VFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSL 62
           +T   KC   +F  +D+ FFN TK  E+ KGA E+NVPI RANRKLVA++NGGL NPS L
Sbjct: 106 KTMEAKCLQGMFELIDSAFFNETKLQEIAKGATEMNVPIYRANRKLVATKNGGLENPSPL 165

Query: 63  VFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRI 122
           VFNP W  E  R + KRF YP  SG+K P +E+DIAFMSVLELGELIKT QI+S+ELVRI
Sbjct: 166 VFNPSWNREVQRVQDKRFKYPSASGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRI 225

Query: 123 FLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLG 161
           +L++LKRYNHVLEAVV++TEELAYKQAKEAD+L +QG YLG
Sbjct: 226 YLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLG 266

The following BLAST results are available for this feature:

BLAST of Cucsat.G849.T25 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RC40	6.2e-05	38.33	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus s...	[more]
B0K3S3	8.1e-05	36.67	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain ...	[more]
B0KBN4	1.4e-04	35.00	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanol...	[more]
Q31F53	1.8e-04	38.33	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Hydrogenovibrio crunogenus (str...	[more]
B6J4H5	8.9e-04	42.37	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain CbuK_...	[more]

BLAST of Cucsat.G849.T25 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650446.1	5.35e-103	100.00	uncharacterized protein LOC101214338 isoform X2 [Cucumis sativus]	[more]
XP_011650445.1	1.05e-102	100.00	uncharacterized protein LOC101214338 isoform X1 [Cucumis sativus] >KAE8650086.1 ...	[more]
TYK19429.1	3.12e-99	95.00	glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo var. makuwa]	[more]
XP_016900589.1	5.45e-99	95.00	PREDICTED: glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo]	[more]
XP_038905194.1	4.01e-91	88.13	glutamyl-tRNA(Gln) amidotransferase subunit A [Benincasa hispida]	[more]

BLAST of Cucsat.G849.T25 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5U7	1.69e-102	100.00	Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G039330 PE=...	[more]
A0A5D3D785	1.51e-99	95.00	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S4DX80	2.64e-99	95.00	glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1CPF2	1.77e-80	79.25	uncharacterized protein LOC111013354 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1G4E4	6.19e-78	82.67	uncharacterized protein LOC111450695 OS=Cucurbita moschata OX=3662 GN=LOC1114506...	[more]

BLAST of Cucsat.G849.T25 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07360.1	3.5e-56	67.08	Amidase family protein	[more]
AT5G07360.2	3.5e-56	67.08	Amidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036928	Amidase signature (AS) superfamily	GENE3D	3.90.1300.10	Amidase signature (AS) domain	coord: 64..160 e-value: 9.7E-13 score: 49.9
IPR036928	Amidase signature (AS) superfamily	SUPERFAMILY	75304	Amidase signature (AS) enzymes	coord: 80..156
None	No IPR available	PANTHER	PTHR11895:SF73	AMIDASE FAMILY PROTEIN	coord: 4..160
IPR000120	Amidase	PANTHER	PTHR11895	TRANSAMIDASE	coord: 4..160

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G849	Cucsat.G849	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G849.T25.E1	Cucsat.G849.T25.E1	exon
Cucsat.G849.T25.E2	Cucsat.G849.T25.E2	exon
Cucsat.G849.T25.E3	Cucsat.G849.T25.E3	exon
Cucsat.G849.T25.E4	Cucsat.G849.T25.E4	exon
Cucsat.G849.T25.E5	Cucsat.G849.T25.E5	exon
Cucsat.G849.T25.E6	Cucsat.G849.T25.E6	exon
Cucsat.G849.T25.E7	Cucsat.G849.T25.E7	exon
Cucsat.G849.T25.E8	Cucsat.G849.T25.E8	exon
Cucsat.G849.T25.E9	Cucsat.G849.T25.E9	exon
Cucsat.G849.T25.E10	Cucsat.G849.T25.E10	exon
Cucsat.G849.T25.E11	Cucsat.G849.T25.E11	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G849.T25.C1	Cucsat.G849.T25.C1	CDS
Cucsat.G849.T25.C2	Cucsat.G849.T25.C2	CDS
Cucsat.G849.T25.C3	Cucsat.G849.T25.C3	CDS
Cucsat.G849.T25.C4	Cucsat.G849.T25.C4	CDS
Cucsat.G849.T25.C5	Cucsat.G849.T25.C5	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G849.T25	Cucsat.G849.T25-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016740	transferase activity