Cucsat.G643.T8 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G643.T8
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	RHOMBOID-like protein
Location	ctg1: 12570745 .. 12573885 (-)
RNA-Seq Expression	Cucsat.G643.T8
Synteny	Cucsat.G643.T8

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTGAAGAAAAAACGCGCTAGTGGTTCAGATTAACCGCGAAAGGAAACTTCTTGTAGTGGATTGAAAGAGTGGGGAATCAACAGAAAGTGAAAGCCCACGTACCCACAGAGCTAATCCACCGTTTTCGTTCCCAATTCACTCGAAATGTAACTCACAACCCCAACCCTCCATTTGTAGATTCAACCAATGTCTGAATTCCTCAATTCGAGTTCTATTTTCTAATTGGGTCCATCTTGCGAGGGCGTACAAAATCGGAATGTAGAGGATTCCTGATCACTATGGCAGTTGATCTCTCTTCCATTTCACTTCATAAAGACCCTTCACCAAACTTCGCCATCCCAACTCACCCACATTCAATTTTCGATTCTCAGGGGGAGGTTGAGTTCAGAGAGACAAAGTATGAACAGGCAGGATATCGAGAGCAGAGAAGTGAAGAACAGAGGAAGTAGTAGCCTCTCTTCTTCCTCTTATTTGATTGAAGATACTGAAACACAATGGACTTCCTGGCTTGTTCCTATGTTTGTAGTGGCTAATATTGCTGTGTTCATTGTGGTTATGTATATTAACAATTGCCCCAAGCATAATGATGAGTTTGAAGGGAAGTGTGTGGCAAGGTTTCTGGAGAGGTTCTCATTTCAGCCTTTGAGGGAAAATCCTCTCTTTGGCCCTTCTGCTACCACGTAAGAGTTGCCTTTGCTTTACTTCCCTTTATACCCATATACAAATTTTCGGTTTCACTTGATTTTCTCTTGTTATATTCCCCTTGGGTTAGAATTATTATCACTGTGTTGCTTGTTTGAGATAATTCAAGTGTGTGTTGTTATTTTCAATATGGGATATGCTTACTTTTAGTTTGTAATTAAGTTTTGATGATTTTGGTTACCTAACAACAACTCCACTCGATGCTGTGTTTACATTATTCTGAAGTTGTTTCTTGTTTAATCATTGTTTGCTGTGGTTACTTTTACTTAAGAGGCTCTGCGATCAATAAGTTTAGTGGCTTTTTGGCGAGTTACTACTTGGGGTGTGGTGGGGTTTTAGTCATACTCTCCTTATTGTTTCTGTTTATTGAATTTCTAAGAGATGTGTAGGTTTTGTTTTCTTTTGTACTTTTTCACAATGCCTTACGAATGATGGATGACTTCCTGGAGTAACTTTATAGATGAGCAGGGACATCAAAGAATATCAGTCAATTCTGAAGAATTTACTGGTAAAAGAATTAGCTTTCGGGTGTATGACAAATAGAATTGAGCTAAGTGGTGAATTTTCGTGGTCCTAAGATGGACAGTTGGAGTGTGTTTAAATTTGAAAAGAACCAACAAATATGGAGTTCCTTATTATATTACAGATTGGAGAAGATGGGAGCTCTAGAGTCAACTAAAGTTGTGAATAAACATCAAGCATGGAGGTTGATTACATGCATCTGGTTACATGCTGGTGTCTTTCATCTGCTTGCAAACATGTTATGCCTGGTCTTTATTGGCACTCGCCTTGAGCAGCAATTTGGGTTTGGTATGTGTTGTTCTTTATTTATTAATTTTTGTTTTGATGAAATGAATCTTCTCAATAAGTAAAGGTAATCAGTGCTTGTAAATTGCTAACGTGAAAGAAGGCATTTCTGCTATTACAAGTCTCTCGTCTTCTATACAACTGCAATTTTAGATTCTTCTCCAGATGGTTAAAGCATCTACTTCTATTTATTTCCTGTTAAATTCTTGCAAGATGTGTTAATATAGAGCATACTATACGTATAATAACTTTTTCGTAAGGCTGTTCTTGGAGAATCAAGCCCAGAGTATATTCTCTTTTCAATCCAACTGTCTTATACCTTTTTCAAGACTATTTTTTGTGACTTAAGTATCTTCACGGTAAGCTGATTTCTTCCTTCTATTTCATGTGCAGTGCGAATTGGAGTTATTTACTTGGTTTCAGGATTTGGAGGGAGTGTACTGTCTTCTTTATTAATTCAGAACAACATCTCTGTGGGTGCTTCTGGTTCTCTTTTTGGTCTTCTTGGAGCTATGCTTTCAGAACTTTTCACAAACTGGACTATCTACACCAACAAGGTTCGTGATTTCAGATCAGAGAGCCAAATGCATTGCCTTTTTCATAATAAACCAACATAGTTTCTCTCACGTTATTTAGGTTTGATCTTCTGATGTATTTGGTGCTTCTTACTTCTATAGGCCGCGGCATTAGCCACACTTCTGGTTATTATTTTTATTAATTTCGGGATTGGACTTCTGCCACATGTTAATAACTTTGCTCACATTGGTGGATTTCTTACTGGATTTCTACTTGGATTTGCATTGCTTCCCCGCCCCAAGTATGGATGGTTAGAGCAACGGAATCTTCCTGGTGTTGGTGCTGGTCTGAGTTCCAAATACAAGACATACCAATATGTTCTATGGATCGTTTCGGTAGTTCTACTGATTGCTGGGTAAGTCCATGTTCATATTTTCTTATGTAGTCTGTGAGATTTATACATAGATGTGGGAAGCACAGCACTGAATCAAAATGGTGACTTTGGTCTTGTTGCTTTTGTATAACTGTCGTAGTATCAGAACAAGATGACTCTTTTGCATGATTCTGTATACCAATTGTTTATAAATATGCAGGTTCACCATTGCTCTAGTGATGCTGTTCCGTGAGGAAAATGCAAACGATCGTTGCCATTGGTGCCATTATTTAAACTGTGTTCCCACTTCAAGATGGCAATGTGATTAGAAACGTCTTTAGAGAGATTTTTCATCATTTGGACCATTCATTTCTCCTGTAAATGTGCAGTTCAGTAGAAATATGGTTAACATTCAGAATTTCTAGTTGCTTGTTGCTTAGGAATAACGATAATGGTGGTAATGAAGACATTTTATTGTTTATGGGTTGTGTTCTCGGCACTAGTAGTAATGGTTTGTTTTGTTGATCGATAAGCAAAACTCAAAGCAACTGCCAGATAAATAATGGATCATACCCTAAACTTCTAGTTTCAATATTTTCCTACAGGGAGACTTTCAATGCCAAGAGCTTGAAAATTATGGATCTTGAAATCAATTTCTGTATATGTTAAAAGTTAAAACTTTTATTGTCAAGGTTAATGCTTAAGACTGCAGAAATTTAGCATTAGGATGCTCAATCATGAC

Coding sequence (CDS)

ATGAACAGGCAGGATATCGAGAGCAGAGAAGTGAAGAACAGAGGAAGTAGTAGCCTCTCTTCTTCCTCTTATTTGATTGAAGATACTGAAACACAATGGACTTCCTGGCTTGTTCCTATGTTTGTAGTGGCTAATATTGCTGTGTTCATTGTGGTTATGTATATTAACAATTGCCCCAAGCATAATGATGAGTTTGAAGGGAAGTGTGTGGCAAGGTTTCTGGAGAGGTTCTCATTTCAGCCTTTGAGGGAAAATCCTCTCTTTGGCCCTTCTGCTACCACATTGGAGAAGATGGGAGCTCTAGAGTCAACTAAAGTTGTGAATAAACATCAAGCATGGAGGTTGATTACATGCATCTGGTTACATGCTGGTGTCTTTCATCTGCTTGCAAACATGTTATGCCTGGTCTTTATTGGCACTCGCCTTGAGCAGCAATTTGGGTTTGTGCGAATTGGAGTTATTTACTTGGTTTCAGGATTTGGAGGGAGTGTACTGTCTTCTTTATTAATTCAGAACAACATCTCTGTGGGTGCTTCTGGTTCTCTTTTTGGTCTTCTTGGAGCTATGCTTTCAGAACTTTTCACAAACTGGACTATCTACACCAACAAGGCCGCGGCATTAGCCACACTTCTGGTTATTATTTTTATTAATTTCGGGATTGGACTTCTGCCACATGTTAATAACTTTGCTCACATTGGTGGATTTCTTACTGGATTTCTACTTGGATTTGCATTGCTTCCCCGCCCCAAGTATGGATGGTTAGAGCAACGGAATCTTCCTGGTGTTGGTGCTGGTCTGAGTTCCAAATACAAGACATACCAATATGTTCTATGGATCGTTTCGGTAGTTCTACTGATTGCTGGGTTCACCATTGCTCTAGTGATGCTGTTCCGTGAGGAAAATGCAAACGATCGTTGCCATTGGTGCCATTATTTAAACTGTGTTCCCACTTCAAGATGGCAATGTGATTAG

Protein sequence

MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPKHNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASGSLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHWCHYLNCVPTSRWQCD

Homology

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BLAST of Cucsat.G643.T8 vs. ExPASy Swiss-Prot
Match: Q9LYP1 (RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.1e-122
Identity = 220/336 (65.48%), Postives = 264/336 (78.57%), Query Frame = 0


Query: 2   NRQDIESREVKNRGSS-----------SLSSSSYLIEDT-ETQWTSWLVPMFVVANIAVF 61
           NR   + R + +RG             +LSSS+   ++   ++WTSWLVPMFVVAN+AVF
Sbjct: 10  NRMSAKDRGIGSRGGDRNRIGPPPLPVALSSSTEFGDNALSSRWTSWLVPMFVVANVAVF 69

Query: 62  IVVMYINNCPKH--NDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVV 121
           +V M++NNCP H  +    G CVA+FL R SF+PLR NPLFGPS+ TLEK+GALE +KVV
Sbjct: 70  VVAMFVNNCPNHFESHRLRGHCVAKFLGRLSFEPLRTNPLFGPSSHTLEKLGALEWSKVV 129

Query: 122 NKHQAWRLITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSS 181
            K + WRL+TCIWLHAGV HL ANML LVFIG RLEQQFGFVRIGVIYL+SG GGSVLSS
Sbjct: 130 EKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFVRIGVIYLLSGIGGSVLSS 189

Query: 182 LLIQNNISVGASGSLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVN 241
           L I+N+ISVGASG+LFGLLG+MLSELFTNWTIY+NK AAL TLL +I IN  IG+LPHV+
Sbjct: 190 LFIRNSISVGASGALFGLLGSMLSELFTNWTIYSNKIAALLTLLFVILINLAIGILPHVD 249

Query: 242 NFAHIGGFLTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIA 301
           NFAH+GGF+TGFLLGF LL RP++ WL + ++P  G  L  KYKTYQY+LW++S+VLLIA
Sbjct: 250 NFAHVGGFVTGFLLGFILLARPQFKWLAREHMP-QGTPLRYKYKTYQYLLWLLSLVLLIA 309

Query: 302 GFTIALVMLFREENANDRCHWCHYLNCVPTSRWQCD 324
           GF +AL+MLFR EN ND C WCHYL CVPTS W+CD
Sbjct: 310 GFVVALLMLFRGENGNDHCRWCHYLRCVPTSSWRCD 344

[Close]

BLAST of Cucsat.G643.T8 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 1.4e-117
Identity = 209/324 (64.51%), Postives = 253/324 (78.09%), Query Frame = 0


Query: 1   MNRQDIESREVKNRGSSSLSSSSYLIEDT--ETQWTSWLVPMFVVANIAVFIVVMYINNC 60
           M  +D+E    KNRG    ++++Y  E++  ET WTSWL+P  VVAN+AVFI VM++N+C
Sbjct: 1   MANRDVERVGKKNRG----ANNNYFYEESSGETHWTSWLIPAIVVANLAVFIAVMFVNDC 60

Query: 61  PKHNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITC 120
           PK       +CVARFL RFSFQPL+ENPLFGPS++TLEKMGALE  KVV++HQ WRL++C
Sbjct: 61  PKKITGPNKECVARFLGRFSFQPLKENPLFGPSSSTLEKMGALEWRKVVHEHQGWRLLSC 120

Query: 121 IWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGA 180
           +WLHAG+ HLL NML L+FIG RLEQQFGF+R+G+IYL+SG GGS+LSSL +Q +ISVGA
Sbjct: 121 MWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVGLIYLISGLGGSILSSLFLQESISVGA 180

Query: 181 SGSLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTG 240
           SG+LFGLLGAMLSEL TNWTIY NKAAAL TLL II IN  +G+LP V+NFAHIGGFLTG
Sbjct: 181 SGALFGLLGAMLSELLTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGFLTG 240

Query: 241 FLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR 300
           F LGF LL RP+YGW   R      +    KY  YQYVL++VSVVLL+ G T+ALVMLF+
Sbjct: 241 FCLGFVLLVRPQYGWEASRTNT---SRTKRKYSMYQYVLFVVSVVLLVVGLTVALVMLFK 300

Query: 301 EENANDRCHWCHYLNCVPTSRWQC 323
            EN N  C WCHYL+C PTS+W C
Sbjct: 301 GENGNKHCKWCHYLSCFPTSKWTC 317

[Close]

BLAST of Cucsat.G643.T8 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 1.9e-109
Identity = 193/292 (66.10%), Postives = 232/292 (79.45%), Query Frame = 0


Query: 33  WTSWLVPMFVVANIAVFIVVMYINNCPKHNDEFEGKCVARFLERFSFQPLRENPLFGPSA 92
           W  WLVP  VVANIA+F + M+INNCPK++      C+ARFL RF+FQP++ENPL GPS+
Sbjct: 54  WFPWLVPAIVVANIALFAISMFINNCPKNS----AYCLARFLGRFAFQPMKENPLLGPSS 113

Query: 93  TTLEKMGALESTKVVNKHQAWRLITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIG 152
            TLEKMGAL+ + VV+KH+ WRL TCIWLHAGVFH+LANML L+FIG RLEQ+FGFVRIG
Sbjct: 114 LTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFVRIG 173

Query: 153 VIYLVSGFGGSVLSSLLIQNNISVGASGSLFGLLGAMLSELFTNWTIYTNKAAALATLLV 212
           ++Y++SGFGGS+LSSL  +  ISVGASG+LFGLLGAMLSEL TNWTIY NK AAL TL+ 
Sbjct: 174 LLYMISGFGGSLLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFAALLTLIF 233

Query: 213 IIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPRPKYGWLEQRNLP-GVGA-GLSSKY 272
           II IN  +G+LPHV+NFAH+GGF +GFLLGF  L RP+YG+  QRN P G  A    SK+
Sbjct: 234 IIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAKSKH 293

Query: 273 KTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHWCHYLNCVPTSRWQC 323
           K YQYVLWI S+VLLIAG+T  LV+L R  + N  C WCHYL+C+PTS W C
Sbjct: 294 KPYQYVLWITSLVLLIAGYTAGLVVLLRGTDLNKHCSWCHYLSCIPTSLWSC 341

[Close]

BLAST of Cucsat.G643.T8 vs. ExPASy Swiss-Prot
Match: Q8VZ48 (RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 PE=2 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 3.1e-104
Identity = 182/293 (62.12%), Postives = 221/293 (75.43%), Query Frame = 0


Query: 30  ETQWTSWLVPMFVVANIAVFIVVMYINNCPKHNDEFEGKCVARFLERFSFQPLRENPLFG 89
           +TQWT+WL P  VVAN+++FIVVMY N+CPK      G CVA+ L RFSFQPLRENP  G
Sbjct: 15  DTQWTAWLTPTIVVANVSIFIVVMYTNDCPKTTTGANGDCVAKLLRRFSFQPLRENPFLG 74

Query: 90  PSATTLEKMGALESTKVVNKHQAWRLITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFV 149
           PS++TLEK+GAL+  KVV  ++ WRLIT +WLHAG+ HL+ NM  ++  G RLEQQFGF+
Sbjct: 75  PSSSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFI 134

Query: 150 RIGVIYLVSGFGGSVLSSLLIQNNISVGASGSLFGLLGAMLSELFTNWTIYTNKAAALAT 209
           RIG+IYL+SGFGGS+LS+L +Q +ISVGASG+L GL+GAMLSEL TNWTIY +K  AL +
Sbjct: 135 RIGLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLS 194

Query: 210 LLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSK 269
            L II IN  IGLLP V+NFAHIGG LTGF LGF LL +P+ GW E RN    GA   SK
Sbjct: 195 FLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSK 254

Query: 270 YKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHWCHYLNCVPTSRWQC 323
           Y   QYVL+ V+ VL++AG T+ LVMLF  EN N  C WCH L+C PTS+W C
Sbjct: 255 YNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKWCHRLDCYPTSKWSC 307

[Close]

BLAST of Cucsat.G643.T8 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 5.8e-103
Identity = 178/325 (54.77%), Postives = 235/325 (72.31%), Query Frame = 0


Query: 5   DIESREVKNRGSSSLSSSSYLIEDTE------TQWTSWLVPMFVVANIAVFIVVMYINNC 64
           D+ES    +    SL+ S    E+          W  WL+P FVVAN+AVF++ MY+NNC
Sbjct: 30  DLESSSSVSGQQRSLTQSRSSYEERGRGVKEFRSWFPWLIPCFVVANVAVFVITMYVNNC 89

Query: 65  PKHNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITC 124
           PK +    G C A FL RFSFQ  RENPL GPS+ TL+ MG L+  KVV   + WRL++C
Sbjct: 90  PKKS----GDCFADFLGRFSFQNTRENPLLGPSSLTLQTMGGLDVKKVVKGDEGWRLLSC 149

Query: 125 IWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGA 184
            WLH GV HLL NML L+FIG R+E++FGF+RIG++YL+SGFGGS+LS+L +++NISVGA
Sbjct: 150 NWLHGGVVHLLMNMLTLLFIGIRMEREFGFIRIGLLYLISGFGGSILSALFLRSNISVGA 209

Query: 185 SGSLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTG 244
           SG++FGLLG MLSE+F NWTIY+NK   + TL++I+ +N G+G+LP V+NFAHIGGF TG
Sbjct: 210 SGAVFGLLGGMLSEIFINWTIYSNKVVTIVTLVLIVAVNLGLGVLPGVDNFAHIGGFATG 269

Query: 245 FLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR 304
           FLLGF LL RP YGW+ QRN PG       ++K YQ +LW +S+++L+AGF + L+ LF 
Sbjct: 270 FLLGFVLLIRPHYGWINQRNGPG---AKPHRFKIYQGILWTISLLILVAGFIVGLISLFN 329

Query: 305 EENANDRCHWCHYLNCVPTSRWQCD 324
             + N+ C WCHYL+CVPTS+W C+
Sbjct: 330 NVDGNEHCSWCHYLSCVPTSKWSCN 347

[Close]

BLAST of Cucsat.G643.T8 vs. NCBI nr
Match: XP_004140785.1 (RHOMBOID-like protein 3 isoform X1 [Cucumis sativus] >KGN57353.1 hypothetical protein Csa_009648 [Cucumis sativus])

HSP 1 Score: 645 bits (1664), Expect = 1.89e-233
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 60
           MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK
Sbjct: 1   MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 60

Query: 61  HNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIW 120
           HNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIW
Sbjct: 61  HNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIW 120

Query: 121 LHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASG 180
           LHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASG
Sbjct: 121 LHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASG 180

Query: 181 SLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFL 240
           SLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFL
Sbjct: 181 SLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFL 240

Query: 241 LGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREE 300
           LGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREE
Sbjct: 241 LGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREE 300

Query: 301 NANDRCHWCHYLNCVPTSRWQCD 323
           NANDRCHWCHYLNCVPTSRWQCD
Sbjct: 301 NANDRCHWCHYLNCVPTSRWQCD 323

[Close]

BLAST of Cucsat.G643.T8 vs. NCBI nr
Match: KAA0033622.1 (RHOMBOID-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 603 bits (1555), Expect = 7.78e-217
Identity = 302/323 (93.50%), Postives = 308/323 (95.36%), Query Frame = 0


Query: 1   MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 60
           M RQDIESREVKNRG+SS+SSS+Y+IEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK
Sbjct: 1   MARQDIESREVKNRGNSSISSSTYVIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 60

Query: 61  HNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIW 120
           HND  EGKCVARFLERFSFQPLRENPLFGPSA TLEKMGALESTKVVNKHQAWRLITCIW
Sbjct: 61  HNDGLEGKCVARFLERFSFQPLRENPLFGPSAATLEKMGALESTKVVNKHQAWRLITCIW 120

Query: 121 LHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASG 180
           LHAGVFHLLANMLCLVFIG RLEQQFGFVRIGVIYLVSGFGGSVLSSL IQNNISVGASG
Sbjct: 121 LHAGVFHLLANMLCLVFIGIRLEQQFGFVRIGVIYLVSGFGGSVLSSLFIQNNISVGASG 180

Query: 181 SLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFL 240
           SLFGLLGAMLSELFTNWTIYTNKA ALATLLVII INFGIG+LPHVNNFAHIGGFLTGFL
Sbjct: 181 SLFGLLGAMLSELFTNWTIYTNKAMALATLLVIIVINFGIGILPHVNNFAHIGGFLTGFL 240

Query: 241 LGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREE 300
           LGF LLPRPKYGWLEQRNLPG   GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR  
Sbjct: 241 LGFVLLPRPKYGWLEQRNLPGGSIGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFRGV 300

Query: 301 NANDRCHWCHYLNCVPTSRWQCD 323
           NANDRCHWCHYLNCV TSRW+CD
Sbjct: 301 NANDRCHWCHYLNCVSTSRWKCD 323

[Close]

BLAST of Cucsat.G643.T8 vs. NCBI nr
Match: XP_008439182.1 (PREDICTED: RHOMBOID-like protein 2 [Cucumis melo])

HSP 1 Score: 603 bits (1555), Expect = 1.13e-216
Identity = 302/323 (93.50%), Postives = 308/323 (95.36%), Query Frame = 0


Query: 1   MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 60
           M RQDIESREVKNRG+SS+SSS+Y+IEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK
Sbjct: 11  MARQDIESREVKNRGNSSISSSTYVIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 70

Query: 61  HNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIW 120
           HND  EGKCVARFLERFSFQPLRENPLFGPSA TLEKMGALESTKVVNKHQAWRLITCIW
Sbjct: 71  HNDGLEGKCVARFLERFSFQPLRENPLFGPSAATLEKMGALESTKVVNKHQAWRLITCIW 130

Query: 121 LHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASG 180
           LHAGVFHLLANMLCLVFIG RLEQQFGFVRIGVIYLVSGFGGSVLSSL IQNNISVGASG
Sbjct: 131 LHAGVFHLLANMLCLVFIGIRLEQQFGFVRIGVIYLVSGFGGSVLSSLFIQNNISVGASG 190

Query: 181 SLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFL 240
           SLFGLLGAMLSELFTNWTIYTNKA ALATLLVII INFGIG+LPHVNNFAHIGGFLTGFL
Sbjct: 191 SLFGLLGAMLSELFTNWTIYTNKAMALATLLVIIVINFGIGILPHVNNFAHIGGFLTGFL 250

Query: 241 LGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREE 300
           LGF LLPRPKYGWLEQRNLPG   GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR  
Sbjct: 251 LGFVLLPRPKYGWLEQRNLPGGSIGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFRGV 310

Query: 301 NANDRCHWCHYLNCVPTSRWQCD 323
           NANDRCHWCHYLNCV TSRW+CD
Sbjct: 311 NANDRCHWCHYLNCVSTSRWKCD 333

[Close]

BLAST of Cucsat.G643.T8 vs. NCBI nr
Match: XP_022984650.1 (RHOMBOID-like protein 2 [Cucurbita maxima])

HSP 1 Score: 590 bits (1522), Expect = 1.00e-211
Identity = 295/328 (89.94%), Postives = 304/328 (92.68%), Query Frame = 0


Query: 1   MNRQDIESREV-----KNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYI 60
           M RQDIESREV     KNRGS+S SSSSY+IEDTETQWTSWLVPMFVVANIAVFIVVMYI
Sbjct: 1   MARQDIESREVAGGVAKNRGSTSFSSSSYVIEDTETQWTSWLVPMFVVANIAVFIVVMYI 60

Query: 61  NNCPKHNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRL 120
           NNCPKHND  EGKCVARFLERFSFQPLRENPLFGPSA TLEKMGALESTKVVNKHQAWRL
Sbjct: 61  NNCPKHNDGLEGKCVARFLERFSFQPLRENPLFGPSAATLEKMGALESTKVVNKHQAWRL 120

Query: 121 ITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNIS 180
           ITCIWLHAGVFHL+ANMLCLVFIG RLEQQFGFVRIGVIYLVSG GGSVLSSL IQNNIS
Sbjct: 121 ITCIWLHAGVFHLIANMLCLVFIGIRLEQQFGFVRIGVIYLVSGLGGSVLSSLFIQNNIS 180

Query: 181 VGASGSLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGF 240
           VGASG+LFGLLGAMLSELFTNWTIYTNKAAAL TLLVII IN GIG+LPHVNNFAH+GGF
Sbjct: 181 VGASGALFGLLGAMLSELFTNWTIYTNKAAALITLLVIIAINLGIGILPHVNNFAHVGGF 240

Query: 241 LTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVM 300
            TGFLLGF LLPRPKYGWLEQRNLPG G GLSSKY TYQYVLWI+S VLLIAGFT+ALVM
Sbjct: 241 FTGFLLGFVLLPRPKYGWLEQRNLPGGGVGLSSKYNTYQYVLWIISAVLLIAGFTVALVM 300

Query: 301 LFREENANDRCHWCHYLNCVPTSRWQCD 323
           LFR ENAN RCHWCHYLNCVPTSRW+CD
Sbjct: 301 LFRGENANSRCHWCHYLNCVPTSRWKCD 328

[Close]

BLAST of Cucsat.G643.T8 vs. NCBI nr
Match: KAG6576737.1 (RHOMBOID-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587 bits (1512), Expect = 3.35e-210
Identity = 293/328 (89.33%), Postives = 302/328 (92.07%), Query Frame = 0


Query: 1   MNRQDIESREV-----KNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYI 60
           M RQDIESREV     KNRGS+S SSSSY+IEDTETQWTSWLVPMFVVANIAVF+VVMYI
Sbjct: 1   MARQDIESREVAGGVAKNRGSTSFSSSSYVIEDTETQWTSWLVPMFVVANIAVFVVVMYI 60

Query: 61  NNCPKHNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRL 120
           NNCPKHND   GKCVARFLERFSFQPLRENPLFGPSA TLEKMGALESTKVVNKHQAWRL
Sbjct: 61  NNCPKHNDGLHGKCVARFLERFSFQPLRENPLFGPSAATLEKMGALESTKVVNKHQAWRL 120

Query: 121 ITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNIS 180
           ITCIWLHAGVFHL+ANMLCLVFIG RLEQQFGFVRIGVIYLVSG GGSVLSSL IQNNIS
Sbjct: 121 ITCIWLHAGVFHLIANMLCLVFIGIRLEQQFGFVRIGVIYLVSGLGGSVLSSLFIQNNIS 180

Query: 181 VGASGSLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGF 240
           VGASG+LFGLLGAMLSELFTNWTIYTNKAAAL TLLVII IN GIG+LPHVNNFAH+GGF
Sbjct: 181 VGASGALFGLLGAMLSELFTNWTIYTNKAAALITLLVIIAINLGIGILPHVNNFAHVGGF 240

Query: 241 LTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVM 300
            TGFLLGF LLPRPKYGWLEQRNLPG G GL SKY TYQYVLWIVS VLLIAGFT+ALVM
Sbjct: 241 FTGFLLGFVLLPRPKYGWLEQRNLPGGGVGLRSKYNTYQYVLWIVSAVLLIAGFTVALVM 300

Query: 301 LFREENANDRCHWCHYLNCVPTSRWQCD 323
           LFR ENAN RCHWCHYLNCVPTSRW+CD
Sbjct: 301 LFRGENANSRCHWCHYLNCVPTSRWKCD 328

[Close]

BLAST of Cucsat.G643.T8 vs. ExPASy TrEMBL
Match: A0A0A0L6G0 (RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G180410 PE=3 SV=1)

HSP 1 Score: 645 bits (1664), Expect = 9.15e-234
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 60
           MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK
Sbjct: 1   MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 60

Query: 61  HNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIW 120
           HNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIW
Sbjct: 61  HNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIW 120

Query: 121 LHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASG 180
           LHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASG
Sbjct: 121 LHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASG 180

Query: 181 SLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFL 240
           SLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFL
Sbjct: 181 SLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFL 240

Query: 241 LGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREE 300
           LGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREE
Sbjct: 241 LGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREE 300

Query: 301 NANDRCHWCHYLNCVPTSRWQCD 323
           NANDRCHWCHYLNCVPTSRWQCD
Sbjct: 301 NANDRCHWCHYLNCVPTSRWQCD 323

[Close]

BLAST of Cucsat.G643.T8 vs. ExPASy TrEMBL
Match: A0A5A7SR04 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G00580 PE=3 SV=1)

HSP 1 Score: 603 bits (1555), Expect = 3.77e-217
Identity = 302/323 (93.50%), Postives = 308/323 (95.36%), Query Frame = 0


Query: 1   MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 60
           M RQDIESREVKNRG+SS+SSS+Y+IEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK
Sbjct: 1   MARQDIESREVKNRGNSSISSSTYVIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 60

Query: 61  HNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIW 120
           HND  EGKCVARFLERFSFQPLRENPLFGPSA TLEKMGALESTKVVNKHQAWRLITCIW
Sbjct: 61  HNDGLEGKCVARFLERFSFQPLRENPLFGPSAATLEKMGALESTKVVNKHQAWRLITCIW 120

Query: 121 LHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASG 180
           LHAGVFHLLANMLCLVFIG RLEQQFGFVRIGVIYLVSGFGGSVLSSL IQNNISVGASG
Sbjct: 121 LHAGVFHLLANMLCLVFIGIRLEQQFGFVRIGVIYLVSGFGGSVLSSLFIQNNISVGASG 180

Query: 181 SLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFL 240
           SLFGLLGAMLSELFTNWTIYTNKA ALATLLVII INFGIG+LPHVNNFAHIGGFLTGFL
Sbjct: 181 SLFGLLGAMLSELFTNWTIYTNKAMALATLLVIIVINFGIGILPHVNNFAHIGGFLTGFL 240

Query: 241 LGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREE 300
           LGF LLPRPKYGWLEQRNLPG   GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR  
Sbjct: 241 LGFVLLPRPKYGWLEQRNLPGGSIGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFRGV 300

Query: 301 NANDRCHWCHYLNCVPTSRWQCD 323
           NANDRCHWCHYLNCV TSRW+CD
Sbjct: 301 NANDRCHWCHYLNCVSTSRWKCD 323

[Close]

BLAST of Cucsat.G643.T8 vs. ExPASy TrEMBL
Match: A0A1S3AYU4 (RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103484054 PE=3 SV=1)

HSP 1 Score: 603 bits (1555), Expect = 5.46e-217
Identity = 302/323 (93.50%), Postives = 308/323 (95.36%), Query Frame = 0


Query: 1   MNRQDIESREVKNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 60
           M RQDIESREVKNRG+SS+SSS+Y+IEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK
Sbjct: 11  MARQDIESREVKNRGNSSISSSTYVIEDTETQWTSWLVPMFVVANIAVFIVVMYINNCPK 70

Query: 61  HNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITCIW 120
           HND  EGKCVARFLERFSFQPLRENPLFGPSA TLEKMGALESTKVVNKHQAWRLITCIW
Sbjct: 71  HNDGLEGKCVARFLERFSFQPLRENPLFGPSAATLEKMGALESTKVVNKHQAWRLITCIW 130

Query: 121 LHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGASG 180
           LHAGVFHLLANMLCLVFIG RLEQQFGFVRIGVIYLVSGFGGSVLSSL IQNNISVGASG
Sbjct: 131 LHAGVFHLLANMLCLVFIGIRLEQQFGFVRIGVIYLVSGFGGSVLSSLFIQNNISVGASG 190

Query: 181 SLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFL 240
           SLFGLLGAMLSELFTNWTIYTNKA ALATLLVII INFGIG+LPHVNNFAHIGGFLTGFL
Sbjct: 191 SLFGLLGAMLSELFTNWTIYTNKAMALATLLVIIVINFGIGILPHVNNFAHIGGFLTGFL 250

Query: 241 LGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREE 300
           LGF LLPRPKYGWLEQRNLPG   GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR  
Sbjct: 251 LGFVLLPRPKYGWLEQRNLPGGSIGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFRGV 310

Query: 301 NANDRCHWCHYLNCVPTSRWQCD 323
           NANDRCHWCHYLNCV TSRW+CD
Sbjct: 311 NANDRCHWCHYLNCVSTSRWKCD 333

[Close]

BLAST of Cucsat.G643.T8 vs. ExPASy TrEMBL
Match: A0A6J1J963 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482870 PE=3 SV=1)

HSP 1 Score: 590 bits (1522), Expect = 4.85e-212
Identity = 295/328 (89.94%), Postives = 304/328 (92.68%), Query Frame = 0


Query: 1   MNRQDIESREV-----KNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYI 60
           M RQDIESREV     KNRGS+S SSSSY+IEDTETQWTSWLVPMFVVANIAVFIVVMYI
Sbjct: 1   MARQDIESREVAGGVAKNRGSTSFSSSSYVIEDTETQWTSWLVPMFVVANIAVFIVVMYI 60

Query: 61  NNCPKHNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRL 120
           NNCPKHND  EGKCVARFLERFSFQPLRENPLFGPSA TLEKMGALESTKVVNKHQAWRL
Sbjct: 61  NNCPKHNDGLEGKCVARFLERFSFQPLRENPLFGPSAATLEKMGALESTKVVNKHQAWRL 120

Query: 121 ITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNIS 180
           ITCIWLHAGVFHL+ANMLCLVFIG RLEQQFGFVRIGVIYLVSG GGSVLSSL IQNNIS
Sbjct: 121 ITCIWLHAGVFHLIANMLCLVFIGIRLEQQFGFVRIGVIYLVSGLGGSVLSSLFIQNNIS 180

Query: 181 VGASGSLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGF 240
           VGASG+LFGLLGAMLSELFTNWTIYTNKAAAL TLLVII IN GIG+LPHVNNFAH+GGF
Sbjct: 181 VGASGALFGLLGAMLSELFTNWTIYTNKAAALITLLVIIAINLGIGILPHVNNFAHVGGF 240

Query: 241 LTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVM 300
            TGFLLGF LLPRPKYGWLEQRNLPG G GLSSKY TYQYVLWI+S VLLIAGFT+ALVM
Sbjct: 241 FTGFLLGFVLLPRPKYGWLEQRNLPGGGVGLSSKYNTYQYVLWIISAVLLIAGFTVALVM 300

Query: 301 LFREENANDRCHWCHYLNCVPTSRWQCD 323
           LFR ENAN RCHWCHYLNCVPTSRW+CD
Sbjct: 301 LFRGENANSRCHWCHYLNCVPTSRWKCD 328

[Close]

BLAST of Cucsat.G643.T8 vs. ExPASy TrEMBL
Match: A0A6J1E4W5 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430803 PE=3 SV=1)

HSP 1 Score: 585 bits (1508), Expect = 6.59e-210
Identity = 291/328 (88.72%), Postives = 303/328 (92.38%), Query Frame = 0


Query: 1   MNRQDIESREV-----KNRGSSSLSSSSYLIEDTETQWTSWLVPMFVVANIAVFIVVMYI 60
           M RQDIESREV     KNRGS+S SSSSY+IEDTETQWTSWLVPMFVVANIAVF+VVMYI
Sbjct: 1   MARQDIESREVAGGVAKNRGSTSFSSSSYVIEDTETQWTSWLVPMFVVANIAVFVVVMYI 60

Query: 61  NNCPKHNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRL 120
           NNCPKHND  +GKCVARFLERFSFQPLRENPLFGPSA TLEKMGALESTKVVNKHQAWRL
Sbjct: 61  NNCPKHNDGLQGKCVARFLERFSFQPLRENPLFGPSAATLEKMGALESTKVVNKHQAWRL 120

Query: 121 ITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNIS 180
           ITCIWLHAGVFHL+ANMLCLVFIG RLEQQFGFVRIGVIY+VSG GGSVLSSL IQNNIS
Sbjct: 121 ITCIWLHAGVFHLIANMLCLVFIGIRLEQQFGFVRIGVIYVVSGLGGSVLSSLFIQNNIS 180

Query: 181 VGASGSLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGF 240
           VGASG+LFGLLGAMLSELFTNWTIYTNKAAAL TLLVII IN GIG+LPHVNNFAH+GGF
Sbjct: 181 VGASGALFGLLGAMLSELFTNWTIYTNKAAALITLLVIIAINLGIGILPHVNNFAHVGGF 240

Query: 241 LTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVM 300
            TGFLLGF LLPRPKYGWLEQRNLPG G GL SKY TYQY+LWIVS VLLIAGFT+ALVM
Sbjct: 241 FTGFLLGFVLLPRPKYGWLEQRNLPGGGVGLRSKYNTYQYLLWIVSAVLLIAGFTVALVM 300

Query: 301 LFREENANDRCHWCHYLNCVPTSRWQCD 323
           LFR ENAN RCHWCHYLNCVPTSRW+CD
Sbjct: 301 LFRGENANSRCHWCHYLNCVPTSRWKCD 328

[Close]

BLAST of Cucsat.G643.T8 vs. TAIR 10
Match: AT5G07250.1 (RHOMBOID-like protein 3 )

HSP 1 Score: 441.0 bits (1133), Expect = 8.0e-124
Identity = 220/336 (65.48%), Postives = 264/336 (78.57%), Query Frame = 0


Query: 2   NRQDIESREVKNRGSS-----------SLSSSSYLIEDT-ETQWTSWLVPMFVVANIAVF 61
           NR   + R + +RG             +LSSS+   ++   ++WTSWLVPMFVVAN+AVF
Sbjct: 10  NRMSAKDRGIGSRGGDRNRIGPPPLPVALSSSTEFGDNALSSRWTSWLVPMFVVANVAVF 69

Query: 62  IVVMYINNCPKH--NDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVV 121
           +V M++NNCP H  +    G CVA+FL R SF+PLR NPLFGPS+ TLEK+GALE +KVV
Sbjct: 70  VVAMFVNNCPNHFESHRLRGHCVAKFLGRLSFEPLRTNPLFGPSSHTLEKLGALEWSKVV 129

Query: 122 NKHQAWRLITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSS 181
            K + WRL+TCIWLHAGV HL ANML LVFIG RLEQQFGFVRIGVIYL+SG GGSVLSS
Sbjct: 130 EKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFVRIGVIYLLSGIGGSVLSS 189

Query: 182 LLIQNNISVGASGSLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVN 241
           L I+N+ISVGASG+LFGLLG+MLSELFTNWTIY+NK AAL TLL +I IN  IG+LPHV+
Sbjct: 190 LFIRNSISVGASGALFGLLGSMLSELFTNWTIYSNKIAALLTLLFVILINLAIGILPHVD 249

Query: 242 NFAHIGGFLTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIA 301
           NFAH+GGF+TGFLLGF LL RP++ WL + ++P  G  L  KYKTYQY+LW++S+VLLIA
Sbjct: 250 NFAHVGGFVTGFLLGFILLARPQFKWLAREHMP-QGTPLRYKYKTYQYLLWLLSLVLLIA 309

Query: 302 GFTIALVMLFREENANDRCHWCHYLNCVPTSRWQCD 324
           GF +AL+MLFR EN ND C WCHYL CVPTS W+CD
Sbjct: 310 GFVVALLMLFRGENGNDHCRWCHYLRCVPTSSWRCD 344

[Close]

BLAST of Cucsat.G643.T8 vs. TAIR 10
Match: AT1G63120.1 (RHOMBOID-like 2 )

HSP 1 Score: 424.1 bits (1089), Expect = 1.0e-118
Identity = 209/324 (64.51%), Postives = 253/324 (78.09%), Query Frame = 0


Query: 1   MNRQDIESREVKNRGSSSLSSSSYLIEDT--ETQWTSWLVPMFVVANIAVFIVVMYINNC 60
           M  +D+E    KNRG    ++++Y  E++  ET WTSWL+P  VVAN+AVFI VM++N+C
Sbjct: 1   MANRDVERVGKKNRG----ANNNYFYEESSGETHWTSWLIPAIVVANLAVFIAVMFVNDC 60

Query: 61  PKHNDEFEGKCVARFLERFSFQPLRENPLFGPSATTLEKMGALESTKVVNKHQAWRLITC 120
           PK       +CVARFL RFSFQPL+ENPLFGPS++TLEKMGALE  KVV++HQ WRL++C
Sbjct: 61  PKKITGPNKECVARFLGRFSFQPLKENPLFGPSSSTLEKMGALEWRKVVHEHQGWRLLSC 120

Query: 121 IWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIGVIYLVSGFGGSVLSSLLIQNNISVGA 180
           +WLHAG+ HLL NML L+FIG RLEQQFGF+R+G+IYL+SG GGS+LSSL +Q +ISVGA
Sbjct: 121 MWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVGLIYLISGLGGSILSSLFLQESISVGA 180

Query: 181 SGSLFGLLGAMLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTG 240
           SG+LFGLLGAMLSEL TNWTIY NKAAAL TLL II IN  +G+LP V+NFAHIGGFLTG
Sbjct: 181 SGALFGLLGAMLSELLTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGFLTG 240

Query: 241 FLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR 300
           F LGF LL RP+YGW   R      +    KY  YQYVL++VSVVLL+ G T+ALVMLF+
Sbjct: 241 FCLGFVLLVRPQYGWEASRTNT---SRTKRKYSMYQYVLFVVSVVLLVVGLTVALVMLFK 300

Query: 301 EENANDRCHWCHYLNCVPTSRWQC 323
            EN N  C WCHYL+C PTS+W C
Sbjct: 301 GENGNKHCKWCHYLSCFPTSKWTC 317

[Close]

BLAST of Cucsat.G643.T8 vs. TAIR 10
Match: AT2G29050.1 (RHOMBOID-like 1 )

HSP 1 Score: 397.1 bits (1019), Expect = 1.3e-110
Identity = 193/292 (66.10%), Postives = 232/292 (79.45%), Query Frame = 0


Query: 33  WTSWLVPMFVVANIAVFIVVMYINNCPKHNDEFEGKCVARFLERFSFQPLRENPLFGPSA 92
           W  WLVP  VVANIA+F + M+INNCPK++      C+ARFL RF+FQP++ENPL GPS+
Sbjct: 54  WFPWLVPAIVVANIALFAISMFINNCPKNS----AYCLARFLGRFAFQPMKENPLLGPSS 113

Query: 93  TTLEKMGALESTKVVNKHQAWRLITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFVRIG 152
            TLEKMGAL+ + VV+KH+ WRL TCIWLHAGVFH+LANML L+FIG RLEQ+FGFVRIG
Sbjct: 114 LTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFVRIG 173

Query: 153 VIYLVSGFGGSVLSSLLIQNNISVGASGSLFGLLGAMLSELFTNWTIYTNKAAALATLLV 212
           ++Y++SGFGGS+LSSL  +  ISVGASG+LFGLLGAMLSEL TNWTIY NK AAL TL+ 
Sbjct: 174 LLYMISGFGGSLLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFAALLTLIF 233

Query: 213 IIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPRPKYGWLEQRNLP-GVGA-GLSSKY 272
           II IN  +G+LPHV+NFAH+GGF +GFLLGF  L RP+YG+  QRN P G  A    SK+
Sbjct: 234 IIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAKSKH 293

Query: 273 KTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHWCHYLNCVPTSRWQC 323
           K YQYVLWI S+VLLIAG+T  LV+L R  + N  C WCHYL+C+PTS W C
Sbjct: 294 KPYQYVLWITSLVLLIAGYTAGLVVLLRGTDLNKHCSWCHYLSCIPTSLWSC 341

[Close]

BLAST of Cucsat.G643.T8 vs. TAIR 10
Match: AT1G12750.1 (RHOMBOID-like protein 6 )

HSP 1 Score: 379.8 bits (974), Expect = 2.2e-105
Identity = 182/293 (62.12%), Postives = 221/293 (75.43%), Query Frame = 0


Query: 30  ETQWTSWLVPMFVVANIAVFIVVMYINNCPKHNDEFEGKCVARFLERFSFQPLRENPLFG 89
           +TQWT+WL P  VVAN+++FIVVMY N+CPK      G CVA+ L RFSFQPLRENP  G
Sbjct: 15  DTQWTAWLTPTIVVANVSIFIVVMYTNDCPKTTTGANGDCVAKLLRRFSFQPLRENPFLG 74

Query: 90  PSATTLEKMGALESTKVVNKHQAWRLITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFV 149
           PS++TLEK+GAL+  KVV  ++ WRLIT +WLHAG+ HL+ NM  ++  G RLEQQFGF+
Sbjct: 75  PSSSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFI 134

Query: 150 RIGVIYLVSGFGGSVLSSLLIQNNISVGASGSLFGLLGAMLSELFTNWTIYTNKAAALAT 209
           RIG+IYL+SGFGGS+LS+L +Q +ISVGASG+L GL+GAMLSEL TNWTIY +K  AL +
Sbjct: 135 RIGLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLS 194

Query: 210 LLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSK 269
            L II IN  IGLLP V+NFAHIGG LTGF LGF LL +P+ GW E RN    GA   SK
Sbjct: 195 FLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSK 254

Query: 270 YKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHWCHYLNCVPTSRWQC 323
           Y   QYVL+ V+ VL++AG T+ LVMLF  EN N  C WCH L+C PTS+W C
Sbjct: 255 YNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKWCHRLDCYPTSKWSC 307

[Close]

BLAST of Cucsat.G643.T8 vs. TAIR 10
Match: AT1G12750.2 (RHOMBOID-like protein 6 )

HSP 1 Score: 379.8 bits (974), Expect = 2.2e-105
Identity = 182/293 (62.12%), Postives = 221/293 (75.43%), Query Frame = 0


Query: 30  ETQWTSWLVPMFVVANIAVFIVVMYINNCPKHNDEFEGKCVARFLERFSFQPLRENPLFG 89
           +TQWT+WL P  VVAN+++FIVVMY N+CPK      G CVA+ L RFSFQPLRENP  G
Sbjct: 15  DTQWTAWLTPTIVVANVSIFIVVMYTNDCPKTTTGANGDCVAKLLRRFSFQPLRENPFLG 74

Query: 90  PSATTLEKMGALESTKVVNKHQAWRLITCIWLHAGVFHLLANMLCLVFIGTRLEQQFGFV 149
           PS++TLEK+GAL+  KVV  ++ WRLIT +WLHAG+ HL+ NM  ++  G RLEQQFGF+
Sbjct: 75  PSSSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFI 134

Query: 150 RIGVIYLVSGFGGSVLSSLLIQNNISVGASGSLFGLLGAMLSELFTNWTIYTNKAAALAT 209
           RIG+IYL+SGFGGS+LS+L +Q +ISVGASG+L GL+GAMLSEL TNWTIY +K  AL +
Sbjct: 135 RIGLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLS 194

Query: 210 LLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSK 269
            L II IN  IGLLP V+NFAHIGG LTGF LGF LL +P+ GW E RN    GA   SK
Sbjct: 195 FLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSK 254

Query: 270 YKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHWCHYLNCVPTSRWQC 323
           Y   QYVL+ V+ VL++AG T+ LVMLF  EN N  C WCH L+C PTS+W C
Sbjct: 255 YNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKWCHRLDCYPTSKWSC 307

The following BLAST results are available for this feature:

BLAST of Cucsat.G643.T8 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LYP1	1.1e-122	65.48	RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1	[more]
Q9CAN1	1.4e-117	64.51	RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1	[more]
Q0WQX7	1.9e-109	66.10	RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1	[more]
Q8VZ48	3.1e-104	62.12	RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 P...	[more]
F4JBM4	5.8e-103	54.77	RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1	[more]

BLAST of Cucsat.G643.T8 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140785.1	1.89e-233	100.00	RHOMBOID-like protein 3 isoform X1 [Cucumis sativus] >KGN57353.1 hypothetical pr...	[more]
KAA0033622.1	7.78e-217	93.50	RHOMBOID-like protein 2 [Cucumis melo var. makuwa]	[more]
XP_008439182.1	1.13e-216	93.50	PREDICTED: RHOMBOID-like protein 2 [Cucumis melo]	[more]
XP_022984650.1	1.00e-211	89.94	RHOMBOID-like protein 2 [Cucurbita maxima]	[more]
KAG6576737.1	3.35e-210	89.33	RHOMBOID-like protein 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cucsat.G643.T8 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6G0	9.15e-234	100.00	RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G180410 PE=3 SV=1	[more]
A0A5A7SR04	3.77e-217	93.50	RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold23...	[more]
A0A1S3AYU4	5.46e-217	93.50	RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103484054 PE=3 SV=1	[more]
A0A6J1J963	4.85e-212	89.94	RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482870 PE=3 SV=1	[more]
A0A6J1E4W5	6.59e-210	88.72	RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430803 PE=3 SV=1	[more]

BLAST of Cucsat.G643.T8 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07250.1	8.0e-124	65.48	RHOMBOID-like protein 3	[more]
AT1G63120.1	1.0e-118	64.51	RHOMBOID-like 2	[more]
AT2G29050.1	1.3e-110	66.10	RHOMBOID-like 1	[more]
AT1G12750.1	2.2e-105	62.12	RHOMBOID-like protein 6	[more]
AT1G12750.2	2.2e-105	62.12	RHOMBOID-like protein 6	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022764	Peptidase S54, rhomboid domain	PFAM	PF01694	Rhomboid	coord: 107..249 e-value: 2.3E-41 score: 141.3
IPR035952	Rhomboid-like superfamily	GENE3D	1.20.1540.10		coord: 32..248 e-value: 2.1E-37 score: 130.6
IPR035952	Rhomboid-like superfamily	SUPERFAMILY	144091	Rhomboid-like	coord: 33..250
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR22936:SF86	RHOMBOID-LIKE PROTEIN 3	coord: 1..323
IPR002610	Peptidase S54, rhomboid	PANTHER	PTHR22936	RHOMBOID-RELATED	coord: 1..323

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G643	Cucsat.G643	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G643.T8.E1	Cucsat.G643.T8.E1	exon
Cucsat.G643.T8.E2	Cucsat.G643.T8.E2	exon
Cucsat.G643.T8.E3	Cucsat.G643.T8.E3	exon
Cucsat.G643.T8.E4	Cucsat.G643.T8.E4	exon
Cucsat.G643.T8.E5	Cucsat.G643.T8.E5	exon
Cucsat.G643.T8.E6	Cucsat.G643.T8.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G643.T8.C5	Cucsat.G643.T8.C5	CDS
Cucsat.G643.T8.C4	Cucsat.G643.T8.C4	CDS
Cucsat.G643.T8.C3	Cucsat.G643.T8.C3	CDS
Cucsat.G643.T8.C2	Cucsat.G643.T8.C2	CDS
Cucsat.G643.T8.C1	Cucsat.G643.T8.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G643.T8	Cucsat.G643.T8-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004252	serine-type endopeptidase activity