Cucsat.G643.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G643.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	RHOMBOID-like protein
Location	ctg1: 12570745 .. 12574019 (-)
RNA-Seq Expression	Cucsat.G643.T4
Synteny	Cucsat.G643.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGGTTGGTGGTGTGAGAAGTGTTTGATCTCACATGGAAAATGACGGGTAAACGACATGTCGTCTCATTGCCATTGGCATGAAATGAATGGTACGATGAGTTTCCGGCTCCCCCACTCTCGCACGTACCTCTGGACTGAAGAAAAAACGCGCTAGTGGTTCAGATTAACCGCGAAAGGAAACTTCTTGTAGTGGATTGAAAGAGTGGGGAATCAACAGAAAGTGAAAGCCCACGTACCCACAGAGCTAATCCACCGTTTTCGTTCCCAATTCACTCGAAATGTAACTCACAACCCCAACCCTCCATTTGTAGATTCAACCAATGTCTGAATTCCTCAATTCGAGTTCTATTTTCTAATTGGGTCCATCTTGCGAGGGCGTACAAAATCGGAATGTAGAGGATTCCTGATCACTATGGCAGTTGATCTCTCTTCCATTTCACTTCATAAAGACCCTTCACCAAACTTCGCCATCCCAACTCACCCACATTCAATTTTCGATTCTCAGGGGGAGGTTGAGTTCAGAGAGACAAAGTATGAACAGGCAGGATATCGAGAGCAGAGAAGTGAAGAACAGAGGAAGTAGTAGCCTCTCTTCTTCCTCTTATTTGATTGAAGATACTGAAACACAATGGACTTCCTGGCTTGTTCCTATGTTTGTAGTGGCTAATATTGCTGTGTTCATTGTGGTTATGTATATTAACAATTGCCCCAAGCATAATGATGAGTTTGAAGGGAAGTGTGTGGCAAGGTTTCTGGAGAGGTTCTCATTTCAGCCTTTGAGGGAAAATCCTCTCTTTGGCCCTTCTGCTACCACGTAAGAGTTGCCTTTGCTTTACTTCCCTTTATACCCATATACAAATTTTCGGTTTCACTTGATTTTCTCTTGTTATATTCCCCTTGGGTTAGAATTATTATCACTGTGTTGCTTGTTTGAGATAATTCAAGTGTGTGTTGTTATTTTCAATATGGGATATGCTTACTTTTAGTTTGTAATTAAGTTTTGATGATTTTGGTTACCTAACAACAACTCCACTCGATGCTGTGTTTACATTATTCTGAAGTTGTTTCTTGTTTAATCATTGTTTGCTGTGGTTACTTTTACTTAAGAGGCTCTGCGATCAATAAGTTTAGTGGCTTTTTGGCGAGTTACTACTTGGGGTGTGGTGGGGTTTTAGTCATACTCTCCTTATTGTTTCTGTTTATTGAATTTCTAAGAGATGTGTAGGTTTTGTTTTCTTTTGTACTTTTTCACAATGCCTTACGAATGATGGATGACTTCCTGGAGTAACTTTATAGATGAGCAGGGACATCAAAGAATATCAGTCAATTCTGAAGAATTTACTGGTAAAAGAATTAGCTTTCGGGTGTATGACAAATAGAATTGAGCTAAGTGGTGAATTTTCGTGGTCCTAAGATGGACAGTTGGAGTGTGTTTAAATTTGAAAAGAACCAACAAATATGGAGTTCCTTATTATATTACAGATTGGAGAAGATGGGAGCTCTAGAGTCAACTAAAGTTGTGAATAAACATCAAGCATGGAGGTTGATTACATGCATCTGGTTACATGCTGGTGTCTTTCATCTGCTTGCAAACATGTTATGCCTGGTCTTTATTGGCACTCGCCTTGAGCAGCAATTTGGGTTTGGTATGTGTTGTTCTTTATTTATTAATTTTTGTTTTGATGAAATGAATCTTCTCAATAAGTAAAGGTAATCAGTGCTTGTAAATTGCTAACGTGAAAGAAGGCATTTCTGCTATTACAAGTCTCTCGTCTTCTATACAACTGCAATTTTAGATTCTTCTCCAGATGGTTAAAGCATCTACTTCTATTTATTTCCTGTTAAATTCTTGCAAGATGTGTTAATATAGAGCATACTATACGTATAATAACTTTTTCGTAAGGCTGTTCTTGGAGAATCAAGCCCAGAGTATATTCTCTTTTCAATCCAACTGTCTTATACCTTTTTCAAGACTATTTTTTGTGACTTAAGTATCTTCACGGTAAGCTGATTTCTTCCTTCTATTTCATGTGCAGTGCGAATTGGAGTTATTTACTTGGTTTCAGGATTTGGAGGGAGTGTACTGTCTTCTTTATTAATTCAGAACAACATCTCTGTGGGTGCTTCTGGTTCTCTTTTTGGTCTTCTTGGAGCTATGCTTTCAGAACTTTTCACAAACTGGACTATCTACACCAACAAGGTTCGTGATTTCAGATCAGAGAGCCAAATGCATTGCCTTTTTCATAATAAACCAACATAGTTTCTCTCACGTTATTTAGGTTTGATCTTCTGATGTATTTGGTGCTTCTTACTTCTATAGGCCGCGGCATTAGCCACACTTCTGGTTATTATTTTTATTAATTTCGGGATTGGACTTCTGCCACATGTTAATAACTTTGCTCACATTGGTGGATTTCTTACTGGATTTCTACTTGGATTTGCATTGCTTCCCCGCCCCAAGTATGGATGGTTAGAGCAACGGAATCTTCCTGGTGTTGGTGCTGGTCTGAGTTCCAAATACAAGACATACCAATATGTTCTATGGATCGTTTCGGTAGTTCTACTGATTGCTGGGTAAGTCCATGTTCATATTTTCTTATGTAGTCTGTGAGATTTATACATAGATGTGGGAAGCACAGCACTGAATCAAAATGGTGACTTTGGTCTTGTTGCTTTTGTATAACTGTCGTAGTATCAGAACAAGATGACTCTTTTGCATGATTCTGTATACCAATTGTTTATAAATATGCAGGTTCACCATTGCTCTAGTGATGCTGTTCCGTGAGGAAAATGCAAACGATCGTTGCCATTGGTGCCATTATTTAAACTGTGTTCCCACTTCAAGATGGCAATGTGATTAGAAACGTCTTTAGAGAGATTTTTCATCATTTGGACCATTCATTTCTCCTGTAAATGTGCAGTTCAGTAGAAATATGGTTAACATTCAGAATTTCTAGTTGCTTGTTGCTTAGGAATAACGATAATGGTGGTAATGAAGACATTTTATTGTTTATGGGTTGTGTTCTCGGCACTAGTAGTAATGGTTTGTTTTGTTGATCGATAAGCAAAACTCAAAGCAACTGCCAGATAAATAATGGATCATACCCTAAACTTCTAGTTTCAATATTTTCCTACAGGGAGACTTTCAATGCCAAGAGCTTGAAAATTATGGATCTTGAAATCAATTTCTGTATATGTTAAAAGTTAAAACTTTTATTGTCAAGGTTAATGCTTAAGACTGCAGAAATTTAGCATTAGGATGCTCAATCATGAC

Coding sequence (CDS)

ATGCTTTCAGAACTTTTCACAAACTGGACTATCTACACCAACAAGGCCGCGGCATTAGCCACACTTCTGGTTATTATTTTTATTAATTTCGGGATTGGACTTCTGCCACATGTTAATAACTTTGCTCACATTGGTGGATTTCTTACTGGATTTCTACTTGGATTTGCATTGCTTCCCCGCCCCAAGTATGGATGGTTAGAGCAACGGAATCTTCCTGGTGTTGGTGCTGGTCTGAGTTCCAAATACAAGACATACCAATATGTTCTATGGATCGTTTCGGTAGTTCTACTGATTGCTGGGTTCACCATTGCTCTAGTGATGCTGTTCCGTGAGGAAAATGCAAACGATCGTTGCCATTGGTGCCATTATTTAAACTGTGTTCCCACTTCAAGATGGCAATGTGATTAG

Protein sequence

MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPRPKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHWCHYLNCVPTSRWQCD

Homology

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BLAST of Cucsat.G643.T4 vs. ExPASy Swiss-Prot
Match: Q9LYP1 (RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 9.7e-52
Identity = 93/135 (68.89%), Postives = 111/135 (82.22%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIY+NK AAL TLL +I IN  IG+LPHV+NFAH+GGF+TGFLLGF LL R
Sbjct: 211 MLSELFTNWTIYSNKIAALLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLAR 270

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           P++ WL + ++P  G  L  KYKTYQY+LW++S+VLLIAGF +AL+MLFR EN ND C W
Sbjct: 271 PQFKWLAREHMP-QGTPLRYKYKTYQYLLWLLSLVLLIAGFVVALLMLFRGENGNDHCRW 330

Query: 121 CHYLNCVPTSRWQCD 136
           CHYL CVPTS W+CD
Sbjct: 331 CHYLRCVPTSSWRCD 344

[Close]

BLAST of Cucsat.G643.T4 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.1e-44
Identity = 87/134 (64.93%), Postives = 99/134 (73.88%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSEL TNWTIY NKAAAL TLL II IN  +G+LP V+NFAHIGGFLTGF LGF LL R
Sbjct: 187 MLSELLTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVR 246

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           P+YGW   R      +    KY  YQYVL++VSVVLL+ G T+ALVMLF+ EN N  C W
Sbjct: 247 PQYGWEASRTNT---SRTKRKYSMYQYVLFVVSVVLLVVGLTVALVMLFKGENGNKHCKW 306

Query: 121 CHYLNCVPTSRWQC 135
           CHYL+C PTS+W C
Sbjct: 307 CHYLSCFPTSKWTC 317

[Close]

BLAST of Cucsat.G643.T4 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 4.4e-44
Identity = 86/136 (63.24%), Postives = 101/136 (74.26%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSEL TNWTIY NK AAL TL+ II IN  +G+LPHV+NFAH+GGF +GFLLGF  L R
Sbjct: 206 MLSELLTNWTIYANKFAALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIR 265

Query: 61  PKYGWLEQRNLP-GVGA-GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRC 120
           P+YG+  QRN P G  A    SK+K YQYVLWI S+VLLIAG+T  LV+L R  + N  C
Sbjct: 266 PQYGYFNQRNNPRGYAAPSAKSKHKPYQYVLWITSLVLLIAGYTAGLVVLLRGTDLNKHC 325

Query: 121 HWCHYLNCVPTSRWQC 135
            WCHYL+C+PTS W C
Sbjct: 326 SWCHYLSCIPTSLWSC 341

[Close]

BLAST of Cucsat.G643.T4 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 3.7e-43
Identity = 75/135 (55.56%), Postives = 101/135 (74.81%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSE+F NWTIY+NK   + TL++I+ +N G+G+LP V+NFAHIGGF TGFLLGF LL R
Sbjct: 216 MLSEIFINWTIYSNKVVTIVTLVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIR 275

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           P YGW+ QRN PG       ++K YQ +LW +S+++L+AGF + L+ LF   + N+ C W
Sbjct: 276 PHYGWINQRNGPG---AKPHRFKIYQGILWTISLLILVAGFIVGLISLFNNVDGNEHCSW 335

Query: 121 CHYLNCVPTSRWQCD 136
           CHYL+CVPTS+W C+
Sbjct: 336 CHYLSCVPTSKWSCN 347

[Close]

BLAST of Cucsat.G643.T4 vs. ExPASy Swiss-Prot
Match: Q8VZ48 (RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 6.5e-40
Identity = 81/134 (60.45%), Postives = 93/134 (69.40%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSEL TNWTIY +K  AL + L II IN  IGLLP V+NFAHIGG LTGF LGF LL +
Sbjct: 174 MLSELLTNWTIYKSKLCALLSFLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQ 233

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           P+ GW E RN    GA   SKY   QYVL+ V+ VL++AG T+ LVMLF  EN N  C W
Sbjct: 234 PQSGWEEFRNSSQYGARARSKYNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKW 293

Query: 121 CHYLNCVPTSRWQC 135
           CH L+C PTS+W C
Sbjct: 294 CHRLDCYPTSKWSC 307

[Close]

BLAST of Cucsat.G643.T4 vs. NCBI nr
Match: XP_031739456.1 (RHOMBOID-like protein 3 isoform X3 [Cucumis sativus])

HSP 1 Score: 281 bits (718), Expect = 3.95e-94
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR
Sbjct: 92  MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 151

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW
Sbjct: 152 PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 211

Query: 121 CHYLNCVPTSRWQCD 135
           CHYLNCVPTSRWQCD
Sbjct: 212 CHYLNCVPTSRWQCD 226

[Close]

BLAST of Cucsat.G643.T4 vs. NCBI nr
Match: XP_011651138.1 (RHOMBOID-like protein 3 isoform X2 [Cucumis sativus] >XP_031739455.1 RHOMBOID-like protein 3 isoform X2 [Cucumis sativus])

HSP 1 Score: 281 bits (718), Expect = 9.50e-94
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR
Sbjct: 118 MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 177

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW
Sbjct: 178 PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 237

Query: 121 CHYLNCVPTSRWQCD 135
           CHYLNCVPTSRWQCD
Sbjct: 238 CHYLNCVPTSRWQCD 252

[Close]

BLAST of Cucsat.G643.T4 vs. NCBI nr
Match: XP_004140785.1 (RHOMBOID-like protein 3 isoform X1 [Cucumis sativus] >KGN57353.1 hypothetical protein Csa_009648 [Cucumis sativus])

HSP 1 Score: 281 bits (718), Expect = 9.75e-93
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR
Sbjct: 189 MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 248

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW
Sbjct: 249 PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 308

Query: 121 CHYLNCVPTSRWQCD 135
           CHYLNCVPTSRWQCD
Sbjct: 309 CHYLNCVPTSRWQCD 323

[Close]

BLAST of Cucsat.G643.T4 vs. NCBI nr
Match: TYK22290.1 (RHOMBOID-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 260 bits (664), Expect = 1.96e-84
Identity = 125/135 (92.59%), Postives = 127/135 (94.07%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIYTNKA ALATLLVII INFGIG+LPHVNNFAHIGGFLTGFLLGF LLPR
Sbjct: 197 MLSELFTNWTIYTNKAMALATLLVIIVINFGIGILPHVNNFAHIGGFLTGFLLGFVLLPR 256

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           PKYGWLEQRNLPG   GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR  NANDRCHW
Sbjct: 257 PKYGWLEQRNLPGGSIGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFRGVNANDRCHW 316

Query: 121 CHYLNCVPTSRWQCD 135
           CHYLNCVPTSRW+CD
Sbjct: 317 CHYLNCVPTSRWKCD 331

[Close]

BLAST of Cucsat.G643.T4 vs. NCBI nr
Match: KAA0033622.1 (RHOMBOID-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 257 bits (656), Expect = 2.49e-83
Identity = 124/135 (91.85%), Postives = 126/135 (93.33%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIYTNKA ALATLLVII INFGIG+LPHVNNFAHIGGFLTGFLLGF LLPR
Sbjct: 189 MLSELFTNWTIYTNKAMALATLLVIIVINFGIGILPHVNNFAHIGGFLTGFLLGFVLLPR 248

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           PKYGWLEQRNLPG   GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR  NANDRCHW
Sbjct: 249 PKYGWLEQRNLPGGSIGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFRGVNANDRCHW 308

Query: 121 CHYLNCVPTSRWQCD 135
           CHYLNCV TSRW+CD
Sbjct: 309 CHYLNCVSTSRWKCD 323

[Close]

BLAST of Cucsat.G643.T4 vs. ExPASy TrEMBL
Match: A0A0A0L6G0 (RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G180410 PE=3 SV=1)

HSP 1 Score: 281 bits (718), Expect = 4.72e-93
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR
Sbjct: 189 MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 248

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW
Sbjct: 249 PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 308

Query: 121 CHYLNCVPTSRWQCD 135
           CHYLNCVPTSRWQCD
Sbjct: 309 CHYLNCVPTSRWQCD 323

[Close]

BLAST of Cucsat.G643.T4 vs. ExPASy TrEMBL
Match: A0A5D3DFY1 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00460 PE=3 SV=1)

HSP 1 Score: 260 bits (664), Expect = 9.48e-85
Identity = 125/135 (92.59%), Postives = 127/135 (94.07%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIYTNKA ALATLLVII INFGIG+LPHVNNFAHIGGFLTGFLLGF LLPR
Sbjct: 197 MLSELFTNWTIYTNKAMALATLLVIIVINFGIGILPHVNNFAHIGGFLTGFLLGFVLLPR 256

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           PKYGWLEQRNLPG   GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR  NANDRCHW
Sbjct: 257 PKYGWLEQRNLPGGSIGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFRGVNANDRCHW 316

Query: 121 CHYLNCVPTSRWQCD 135
           CHYLNCVPTSRW+CD
Sbjct: 317 CHYLNCVPTSRWKCD 331

[Close]

BLAST of Cucsat.G643.T4 vs. ExPASy TrEMBL
Match: A0A5A7SR04 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G00580 PE=3 SV=1)

HSP 1 Score: 257 bits (656), Expect = 1.21e-83
Identity = 124/135 (91.85%), Postives = 126/135 (93.33%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIYTNKA ALATLLVII INFGIG+LPHVNNFAHIGGFLTGFLLGF LLPR
Sbjct: 189 MLSELFTNWTIYTNKAMALATLLVIIVINFGIGILPHVNNFAHIGGFLTGFLLGFVLLPR 248

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           PKYGWLEQRNLPG   GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR  NANDRCHW
Sbjct: 249 PKYGWLEQRNLPGGSIGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFRGVNANDRCHW 308

Query: 121 CHYLNCVPTSRWQCD 135
           CHYLNCV TSRW+CD
Sbjct: 309 CHYLNCVSTSRWKCD 323

[Close]

BLAST of Cucsat.G643.T4 vs. ExPASy TrEMBL
Match: A0A1S3AYU4 (RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103484054 PE=3 SV=1)

HSP 1 Score: 257 bits (656), Expect = 1.65e-83
Identity = 124/135 (91.85%), Postives = 126/135 (93.33%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIYTNKA ALATLLVII INFGIG+LPHVNNFAHIGGFLTGFLLGF LLPR
Sbjct: 199 MLSELFTNWTIYTNKAMALATLLVIIVINFGIGILPHVNNFAHIGGFLTGFLLGFVLLPR 258

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           PKYGWLEQRNLPG   GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFR  NANDRCHW
Sbjct: 259 PKYGWLEQRNLPGGSIGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFRGVNANDRCHW 318

Query: 121 CHYLNCVPTSRWQCD 135
           CHYLNCV TSRW+CD
Sbjct: 319 CHYLNCVSTSRWKCD 333

[Close]

BLAST of Cucsat.G643.T4 vs. ExPASy TrEMBL
Match: A0A6J1J963 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482870 PE=3 SV=1)

HSP 1 Score: 254 bits (649), Expect = 1.62e-82
Identity = 119/135 (88.15%), Postives = 124/135 (91.85%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIYTNKAAAL TLLVII IN GIG+LPHVNNFAH+GGF TGFLLGF LLPR
Sbjct: 194 MLSELFTNWTIYTNKAAALITLLVIIAINLGIGILPHVNNFAHVGGFFTGFLLGFVLLPR 253

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           PKYGWLEQRNLPG G GLSSKY TYQYVLWI+S VLLIAGFT+ALVMLFR ENAN RCHW
Sbjct: 254 PKYGWLEQRNLPGGGVGLSSKYNTYQYVLWIISAVLLIAGFTVALVMLFRGENANSRCHW 313

Query: 121 CHYLNCVPTSRWQCD 135
           CHYLNCVPTSRW+CD
Sbjct: 314 CHYLNCVPTSRWKCD 328

[Close]

BLAST of Cucsat.G643.T4 vs. TAIR 10
Match: AT5G07250.1 (RHOMBOID-like protein 3 )

HSP 1 Score: 204.1 bits (518), Expect = 6.9e-53
Identity = 93/135 (68.89%), Postives = 111/135 (82.22%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSELFTNWTIY+NK AAL TLL +I IN  IG+LPHV+NFAH+GGF+TGFLLGF LL R
Sbjct: 211 MLSELFTNWTIYSNKIAALLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLAR 270

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           P++ WL + ++P  G  L  KYKTYQY+LW++S+VLLIAGF +AL+MLFR EN ND C W
Sbjct: 271 PQFKWLAREHMP-QGTPLRYKYKTYQYLLWLLSLVLLIAGFVVALLMLFRGENGNDHCRW 330

Query: 121 CHYLNCVPTSRWQCD 136
           CHYL CVPTS W+CD
Sbjct: 331 CHYLRCVPTSSWRCD 344

[Close]

BLAST of Cucsat.G643.T4 vs. TAIR 10
Match: AT1G63120.1 (RHOMBOID-like 2 )

HSP 1 Score: 180.6 bits (457), Expect = 8.2e-46
Identity = 87/134 (64.93%), Postives = 99/134 (73.88%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSEL TNWTIY NKAAAL TLL II IN  +G+LP V+NFAHIGGFLTGF LGF LL R
Sbjct: 187 MLSELLTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVR 246

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           P+YGW   R      +    KY  YQYVL++VSVVLL+ G T+ALVMLF+ EN N  C W
Sbjct: 247 PQYGWEASRTNT---SRTKRKYSMYQYVLFVVSVVLLVVGLTVALVMLFKGENGNKHCKW 306

Query: 121 CHYLNCVPTSRWQC 135
           CHYL+C PTS+W C
Sbjct: 307 CHYLSCFPTSKWTC 317

[Close]

BLAST of Cucsat.G643.T4 vs. TAIR 10
Match: AT2G29050.1 (RHOMBOID-like 1 )

HSP 1 Score: 178.7 bits (452), Expect = 3.1e-45
Identity = 86/136 (63.24%), Postives = 101/136 (74.26%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSEL TNWTIY NK AAL TL+ II IN  +G+LPHV+NFAH+GGF +GFLLGF  L R
Sbjct: 206 MLSELLTNWTIYANKFAALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIR 265

Query: 61  PKYGWLEQRNLP-GVGA-GLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRC 120
           P+YG+  QRN P G  A    SK+K YQYVLWI S+VLLIAG+T  LV+L R  + N  C
Sbjct: 266 PQYGYFNQRNNPRGYAAPSAKSKHKPYQYVLWITSLVLLIAGYTAGLVVLLRGTDLNKHC 325

Query: 121 HWCHYLNCVPTSRWQC 135
            WCHYL+C+PTS W C
Sbjct: 326 SWCHYLSCIPTSLWSC 341

[Close]

BLAST of Cucsat.G643.T4 vs. TAIR 10
Match: AT3G53780.1 (RHOMBOID-like protein 4 )

HSP 1 Score: 175.6 bits (444), Expect = 2.6e-44
Identity = 75/135 (55.56%), Postives = 101/135 (74.81%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSE+F NWTIY+NK   + TL++I+ +N G+G+LP V+NFAHIGGF TGFLLGF LL R
Sbjct: 92  MLSEIFINWTIYSNKVVTIVTLVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIR 151

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           P YGW+ QRN PG       ++K YQ +LW +S+++L+AGF + L+ LF   + N+ C W
Sbjct: 152 PHYGWINQRNGPG---AKPHRFKIYQGILWTISLLILVAGFIVGLISLFNNVDGNEHCSW 211

Query: 121 CHYLNCVPTSRWQCD 136
           CHYL+CVPTS+W C+
Sbjct: 212 CHYLSCVPTSKWSCN 223

[Close]

BLAST of Cucsat.G643.T4 vs. TAIR 10
Match: AT3G53780.2 (RHOMBOID-like protein 4 )

HSP 1 Score: 175.6 bits (444), Expect = 2.6e-44
Identity = 75/135 (55.56%), Postives = 101/135 (74.81%), Query Frame = 0


Query: 1   MLSELFTNWTIYTNKAAALATLLVIIFINFGIGLLPHVNNFAHIGGFLTGFLLGFALLPR 60
           MLSE+F NWTIY+NK   + TL++I+ +N G+G+LP V+NFAHIGGF TGFLLGF LL R
Sbjct: 216 MLSEIFINWTIYSNKVVTIVTLVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIR 275

Query: 61  PKYGWLEQRNLPGVGAGLSSKYKTYQYVLWIVSVVLLIAGFTIALVMLFREENANDRCHW 120
           P YGW+ QRN PG       ++K YQ +LW +S+++L+AGF + L+ LF   + N+ C W
Sbjct: 276 PHYGWINQRNGPG---AKPHRFKIYQGILWTISLLILVAGFIVGLISLFNNVDGNEHCSW 335

Query: 121 CHYLNCVPTSRWQCD 136
           CHYL+CVPTS+W C+
Sbjct: 336 CHYLSCVPTSKWSCN 347

The following BLAST results are available for this feature:

BLAST of Cucsat.G643.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LYP1	9.7e-52	68.89	RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1	[more]
Q9CAN1	1.1e-44	64.93	RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1	[more]
Q0WQX7	4.4e-44	63.24	RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1	[more]
F4JBM4	3.7e-43	55.56	RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1	[more]
Q8VZ48	6.5e-40	60.45	RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 P...	[more]

BLAST of Cucsat.G643.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739456.1	3.95e-94	100.00	RHOMBOID-like protein 3 isoform X3 [Cucumis sativus]	[more]
XP_011651138.1	9.50e-94	100.00	RHOMBOID-like protein 3 isoform X2 [Cucumis sativus] >XP_031739455.1 RHOMBOID-li...	[more]
XP_004140785.1	9.75e-93	100.00	RHOMBOID-like protein 3 isoform X1 [Cucumis sativus] >KGN57353.1 hypothetical pr...	[more]
TYK22290.1	1.96e-84	92.59	RHOMBOID-like protein 2 [Cucumis melo var. makuwa]	[more]
KAA0033622.1	2.49e-83	91.85	RHOMBOID-like protein 2 [Cucumis melo var. makuwa]	[more]

BLAST of Cucsat.G643.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6G0	4.72e-93	100.00	RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G180410 PE=3 SV=1	[more]
A0A5D3DFY1	9.48e-85	92.59	RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold44...	[more]
A0A5A7SR04	1.21e-83	91.85	RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold23...	[more]
A0A1S3AYU4	1.65e-83	91.85	RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103484054 PE=3 SV=1	[more]
A0A6J1J963	1.62e-82	88.15	RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482870 PE=3 SV=1	[more]

BLAST of Cucsat.G643.T4 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07250.1	6.9e-53	68.89	RHOMBOID-like protein 3	[more]
AT1G63120.1	8.2e-46	64.93	RHOMBOID-like 2	[more]
AT2G29050.1	3.1e-45	63.24	RHOMBOID-like 1	[more]
AT3G53780.1	2.6e-44	55.56	RHOMBOID-like protein 4	[more]
AT3G53780.2	2.6e-44	55.56	RHOMBOID-like protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022764	Peptidase S54, rhomboid domain	PFAM	PF01694	Rhomboid	coord: 2..61 e-value: 3.6E-9 score: 36.8
IPR035952	Rhomboid-like superfamily	GENE3D	1.20.1540.10		coord: 1..60 e-value: 8.1E-7 score: 30.8
IPR035952	Rhomboid-like superfamily	SUPERFAMILY	144091	Rhomboid-like	coord: 5..62
None	No IPR available	PANTHER	PTHR22936:SF86	RHOMBOID-LIKE PROTEIN 3	coord: 1..135
IPR002610	Peptidase S54, rhomboid	PANTHER	PTHR22936	RHOMBOID-RELATED	coord: 1..135

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G643	Cucsat.G643	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G643.T4.E1	Cucsat.G643.T4.E1	exon
Cucsat.G643.T4.E2	Cucsat.G643.T4.E2	exon
Cucsat.G643.T4.E3	Cucsat.G643.T4.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G643.T4.C3	Cucsat.G643.T4.C3	CDS
Cucsat.G643.T4.C2	Cucsat.G643.T4.C2	CDS
Cucsat.G643.T4.C1	Cucsat.G643.T4.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G643.T4	Cucsat.G643.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004252	serine-type endopeptidase activity