Cucsat.G5368.T6 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G5368.T6
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	nucleolar MIF4G domain-containing protein 1
Location	ctg1267: 280616 .. 285777 (-)
RNA-Seq Expression	Cucsat.G5368.T6
Synteny	Cucsat.G5368.T6

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAAATTGGATGGAAAATAGATGGACAACGAGCGGGAGGGCACTGTTAGTCTAAATCTCAATTCGGCCTTCGGAAGAGTCATCCCCTTCTCCGCAAAAACCCCTTTCCTAAACCCCTCCACCCACCGGGCACCAGTCACCACCCTCTTCATCACCCTTCTTCTCTATCCCCCTTACTCACCGACGCCGCTTCTCTCTCTCTCTCTCTCTCTCTCTCTTTTCATCTTTTTTCTTCACCTCAAAATTGGTTTCATTTGCCTTTTCAAATACCCCAATTTCATAACTGAAACTGTGTCTTCGCCGGTAGTGTAAACAGGAAGCAAGGGGGAATGGGGAAGGAGGATGCGAAGCACCGGTCTCGGACCAAGTACAAGGGAAAAACGAAATTCGAGGAGTATTTGGATATGGAAACGGGCACGCGAAACCCTGGGTTATCTGCTGAGGAGGACTTGGAATTGGAACGAAAGCTTGCAAAGAAATTGAAGGTCAAGGCTGGGAAGTTAAGGGGAGTGGATGATGGTATCAATGTGTTATTTGAAGGAATCCCTTCCATTGTTGATTTCCCTGGGGAGGAAGTATTGCAGTTTTCTGAGGAGTGCGCTGTTGAGGAAACTAAGAAAAATCCATTAGGTAAAAAGGGGAACAGAAGGAAATCATTAGATCAAGCCCTAGATATGGAGAGTGAGTTAACTACTGTTGTTGAGGAGGAAAATGCTTCAAAGAAAAATAAGAAACGTAAGAAGAAGAAGAGGATATCTGAAAAACTTGACGATGTAGTGACAGAGGATTCCGCTGGTGATGAATCTATGCCTGTGGAATCACATTGTGTAGAGGCTGCAGTGGATAAAGTTCCTCCCAAAGCTCAAAAATATGTTGCTCCTTACTTGAGATTGCAAAAAGGAGCTGAGCCTGAAGATCATACTCAACTCCGTCGGCGTGTTCGAGGTGTGGAGGTTGTATGATTATGCGTTCATTTTTTTCCCCTTTCTTGTTTTTTAATTTGGATGTAATTTGCGTTGTATTGATTTTTCATCAGGTCTTCTAAATAGGCTCTCTGAATCAAATATCGAATCGGTTACAGGGGAAATGTCTACGGTTTTTCATGTATGATGTTATCTTCTAACTATCTCGTTACCTATGTTTCTTGAATGTCTATTACAGCCTTACTGAGCGCTTGTAACTTGTGATCAATGGTATCTAGTCTCTATTTGATCTAAGCTCGTCAGTGAATCTATTGTGTTGTATCAATTTGCAGTCTATTTCTCGCAGCATTGCTTCTCAAATTATTGGTGATGAGATTCTGGCATCTTGTTCACGTGGTCCTCGAGGCAATGAACAGTTAAGTGTTGTTTTATATTTTTATTTGGCACTTCTATCTATTGTTGTTTATATATGTTGAATCCTTTTCTTTTTTAAAATATATAGTTAGATTTTATTTTGATAAAAGTTTTATATTTAACTTCTCATTCATCACTTCACTATAACTTTTTTATTATAGTTAAGAACATTGGTTGACACAATGTTGAGGAGTTTCTGCTGAATTGGTCAAATTTGAAACTTTGTATAATGATAACATGTTGACCGTTTGTTTTTTGGGTTTCTCTAACTTTTGACAAACTCCTTTGTTTGTTTGTTTCTTCTTGTTCAAGGTATGCTGCTGTATTTGCCTCTTTTGTTGCAGGCATGGGTTGTTTGGTTGGTACTGACTTTAGCGCAAGGCTCATGGCTTCCCTTGCTAAAACATTTGAGGTGAGCTTGCCTGCTAAATACATCATTTAATTATTTGGTTTTGCATGGCTGTAGAGACCTCTACTAAAGTGATAAATACTTTTTTTTCAGGATGAATACCTCAATGAAGATAATCTTTCTTTGCGGAACCTTACACTATTGCTTTCCTATTTATGTGTATTTGGGGTTTGCGCCAGGTAAGTACATATATCCACACATTATGTATTATTCACGATTTTTTTTTCTTTTTGTTTGTTTTTCAAATATCAGATAATTGTGTGTTTTAGTTTGTATAATCTATTTCATTATACTGGATGCATGTGATAGACCTCCCATCATATACCAATATAATAGAAGAAAGAAAGTTAGTTAGTAGACTGTTAGCCAATACAGTTCGTCAGGAAGTTAAGAAGTTAGTTAACAGTTAGTCAGGAAGTTAAGAAGTTAGCTGGTACCGTTAGTGCTATGGTAATAAAAAGAGAGGCTAAGAAAGAAAGAGGCAGGAAAATTATAGTGAGTAAATAGAGAGAGACTTCTGTGCATCTTTGAAAGACAAGATACAGAAGAGCAGAATTCCCTACATGTAAAGTCATTGACTAGATACTATTTTAAAAGATTACTTCATAATACATACGGTTCCCATCAGCGTGCACGTCTTTCTAATAAGCATTTTTTCTAAATACTTTTCAGTGATCTGATCTATGATTTTCTAATTATACTGAGCAAGCGGTTGACAGAAATTGATGTATCCACCATCCTGACAGTTTTACAATGTAAGTTGATTTCAAGCTTGATATGTTTAGATATAGCACCATCTCCTTGTTAATCCACTACTAGAGTTTCTCTTTAAATTGGAATTTTGTCTTGTTTAAATGTCAAGATGTTAAATATATTCTAAATTCATTATCCCTTTTTCTAATCGTTCCTAGATATTTCCTGAACATTAGCAGACTCAGGGCTGTGCATTATGTTAAAAGTTGTGCTTGGATTTAACCACCGGTGATGTTTCTTTTTCAAAAAAAAAAAAGAAAAGATCGAACCACTTGTGATGGATATTTAGTAGTTCATAAATTTTGTCAATAACAAAGGCTGCTGACTTAGATGGTTGTTCACTTTCAAATATTGAATTTGACTATTATATCGTTGATGGATGTTCATATCATGGGTTGAATATTCTTAGCTTCTCAATTCTTTTATATTCTAACAAAATTATTGGTCTTGAACTCTTAATATATTACAACAAATTGATGGTCATGGGAAGTTGATATATAACTTAAATCGTCTCTAATTTGGTAGGTTGTGGAATGAAAATAAGGGCTGATGATCCCACTGCAATGAAGAGTTTTATTGTCAGTGTTCAAAGTAAGGTTAATGAGCTAAAGGCAGCTTCTGGAGATGGTCTACAAAATATCAATGGGAAGAGAGTGAGTCTCTATAAGTAGTATTTCATTTGCTGAGTTTTTAAATTTTTAAAATTTTGATTTTCTAATATTACAACGTGGATGCATGCAGATGGAGTTCATGCTGGAGACTATATGTGACATCAAGAATAACAAGAAAAGATCTAAGGATGATCCAGCCCACCATACAAGGATAAAGAAATGGCTACAGAAGGTCTACCTTATTCTATTTCATTGGCTAGCATTGCATGTTTGTGGTTACATTATTTTGTAGTGCGTAGATCTTAATTTGCTAGATAATTGCTCTTTATAGAGTAAACATTTTACTTCAAAAGCAGACTTGTTCACTTGAAAGGTCAAGTTGGATAATTTTTTGTAGGTCTAATGCAAGGACAGTGAAAGATTTTCAATTAGTCTCTTTTTGAGCGCATGAAACATTTTTAATATCTTAGTCAAGTAGTAACCATCATGGGTTGACCTAGTCGTAAAAAGGTCAATAACTAAGAGGTCATGGCATGGTGGCCACCTACCTAGGAATTAATTTCTTCTGGGTTTTCTTGACACCCAAATGTTGTAGGGTCAGGTGGGTTGTCCCGTGAGATTAGTTGAAGTGTGCGTAAGCAGGCTTGGACACTCACGGATATGAAAAAAAAATCTTAGTCAAGTAGTCATGATGACTTTTATTCTCCCAAAAATGTTTCTTACTTTTTGCATGTTGTAATGCTATTAGGCCAACTCATCGATAGTTAAACAAAAAGTGGGATTCTATGCTTGAACTACCTAACCTTGCATTGTTGAGTGTGATTGAAATCTGACCTTTTTTTAAGCCTCATTCTTCAATTATTATTTTCAGTTAGGAGTAGATGATATAATAATTAGAGGGATCAAATGGAACAAGTTGCTTGATCCTGACAAGAAGGGGCAGTGGTGGTTGTCTGGGGATTTGTCAACGACATCAGATAACGTTGAAGAGTTTGCCAACACTATTGATAAAGAGGTACTTGAAGCTCAAAAAATGCTTCAGCTTGCAGCTGCACAAAGAATGAATACAGATGCTAGGAAAGCGATATTTTGTATTATAATGAGTGGAGAGGACTACGTTGATGCATTTGAGAAGCTAATTAGGTTGGATTTATCTGGAAAGCAGGTTGGTGGAATTTTGTAAATTTTCTTCACCCTACCTCCAAATCATACCTCTTATTGTGCTTACCATCTCCATGTCGTCTACAGGACCGGGAGATCATGAGGGTTCTTGTGGACTGTTGTTTACAAGAGAAAGTGTTTAACAAGTACTACACTGTTCTAGCTTCCAAATTGTGTGAGCATGAAAAGAACCACAAGTTTACATTACAGGTAAGTTCTTTGCCTCTTTGGTCTGTACTCAGTTTGGTTAATGAAACTTTAGATACGGTTGCGAAAGTACCATCTTCTAACTACGCCACACCTCATCTTCACAGTATTGCTTGTGGGATCAATTCAAAGAACTGGATACGATGCAACTTATCAGGTCGATGAATCTTGCGAAGTTTGTCGCCGAGATGATCACCTCTTTTACTCTCTCACTTGCTGTCTTGAAGTCAGTAGATTTGAGTGATATTAGGCTGCTAACTGCAAAGAGAATCATGCATTTTCGTATGCTGTTTGATGCAATTTTTGAACGTCCTGACAAGCTGATATGGAACGTGTTCACTCGAGTTGCTGTTAACCCTGAGCTCGAACCTCTTCGAAGTGGTATGTTGTTCTTCATTAAAGAGTACATGATTAAAACTAGTAAAGTTAATGCAGAGAAGTTTAAGCTTATCAAGAAAGCGCTTAACAATGTTGAGGGAATACTAATGTGAATTTTGGAGTGAGAATTAGAGTTTTCAAAGTTCTCTTTTTGATCTTACTTATAGAGAAGAGGGATGTGTTGTACACTGTCATGTTTTGGCTAAATGAAAGAATAAACTGCTATTTTGTAACTCAAATTATTTCTTGAAGGTTTTAAATCTTGGAGTAAATTACTCATACGGGTTTGATACTTATTTTTCTTTGT

Coding sequence (CDS)

ATGGGGAAGGAGGATGCGAAGCACCGGTCTCGGACCAAGTACAAGGGAAAAACGAAATTCGAGGAGTATTTGGATATGGAAACGGGCACGCGAAACCCTGGGTTATCTGCTGAGGAGGACTTGGAATTGGAACGAAAGCTTGCAAAGAAATTGAAGGTCAAGGCTGGGAAGTTAAGGGGAGTGGATGATGGTATCAATGTGTTATTTGAAGGAATCCCTTCCATTGTTGATTTCCCTGGGGAGGAAGTATTGCAGTTTTCTGAGGAGTGCGCTGTTGAGGAAACTAAGAAAAATCCATTAGGTAAAAAGGGGAACAGAAGGAAATCATTAGATCAAGCCCTAGATATGGAGAGTGAGTTAACTACTGTTGTTGAGGAGGAAAATGCTTCAAAGAAAAATAAGAAACGTAAGAAGAAGAAGAGGATATCTGAAAAACTTGACGATGTAGTGACAGAGGATTCCGCTGGTGATGAATCTATGCCTGTGGAATCACATTGTGTAGAGGCTGCAGTGGATAAAGTTCCTCCCAAAGCTCAAAAATATGTTGCTCCTTACTTGAGATTGCAAAAAGGAGCTGAGCCTGAAGATCATACTCAACTCCGTCGGCGTGTTCGAGGTCTTCTAAATAGGCTCTCTGAATCAAATATCGAATCGGTTACAGGGGAAATGTCTACGGTTTTTCATTCTATTTCTCGCAGCATTGCTTCTCAAATTATTGGTGATGAGATTCTGGCATCTTGTTCACGTGGTCCTCGAGGCAATGAACAGTTAAGTGTTGTTTTATATTTTTATTTGGCACTTCTATCTATTGTTGTTTATATATGTTGA

Protein sequence

MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRGVDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESELTTVVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQKYVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQIIGDEILASCSRGPRGNEQLSVVLYFYLALLSIVVYIC

Homology

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BLAST of Cucsat.G5368.T6 vs. ExPASy Swiss-Prot
Match: Q5C9Z4 (Nucleolar MIF4G domain-containing protein 1 OS=Homo sapiens OX=9606 GN=NOM1 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 6.9e-04
Identity = 60/241 (24.90%), Postives = 113/241 (46.89%), Query Frame = 0


Query: 38  EEDLE---LERKLA-KKLKVKAGK----LRGVDDGINVLFEGIPS-----IVDFPGEEV- 97
           EED E   LER L   K K K G     L    DG++ +   + S     + D  GEE  
Sbjct: 181 EEDREIRKLERCLGLNKRKKKDGSSSVPLSFARDGLDYILGALESGKNSGLYDSSGEEEE 240

Query: 98  ----------LQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESELTTVVEEENASKKN 157
                     L+   +   EE ++  + K+   +++  Q+ D + +     EEE   +K 
Sbjct: 241 DAGQTLPESDLESDSQDESEEEEEGDVEKEKKAQEAEAQSEDDDED----TEEEQGEEKE 300

Query: 158 K---KRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQKYVAPYLRLQK 217
           K   ++++ KR+    D+  +E+S+ D  +  +S C            +KY+ P++R  +
Sbjct: 301 KGAQEKRRGKRVRFAEDEEKSENSSEDGDITDKSLC---------GSGEKYIPPHVRQAE 360

Query: 218 GA----EPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQIIGDEILAS 248
                 + E+  +L++ V+GLLNRLSE N+ S++G++  ++ + SR   +  +   ++ +
Sbjct: 361 ETVDFKKKEELERLKKHVKGLLNRLSEPNMASISGQLEELYMAHSRKDMNDTLTSALMGA 408

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BLAST of Cucsat.G5368.T6 vs. NCBI nr
Match: XP_004149114.1 (nucleolar MIF4G domain-containing protein 1 [Cucumis sativus] >KGN53923.1 hypothetical protein Csa_019148 [Cucumis sativus])

HSP 1 Score: 486 bits (1252), Expect = 3.61e-166
Identity = 258/266 (96.99%), Postives = 261/266 (98.12%), Query Frame = 0


Query: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60
           MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG
Sbjct: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60

Query: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL 120
           VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL
Sbjct: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL 120

Query: 121 TTVVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQK 180
           TTVVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQK
Sbjct: 121 TTVVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQK 180

Query: 181 YVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQIIG 240
           YVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQIIG
Sbjct: 181 YVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQIIG 240

Query: 241 DEILASCSRGPRGNEQLSVVLYFYLA 266
           DEILASCSRGPRGNEQ + V   ++A
Sbjct: 241 DEILASCSRGPRGNEQYAAVFASFVA 266

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BLAST of Cucsat.G5368.T6 vs. NCBI nr
Match: XP_008441997.1 (PREDICTED: nucleolar MIF4G domain-containing protein 1 [Cucumis melo] >KAA0041729.1 nucleolar MIF4G domain-containing protein 1 [Cucumis melo var. makuwa] >TYK08400.1 nucleolar MIF4G domain-containing protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 441 bits (1134), Expect = 2.45e-148
Identity = 239/267 (89.51%), Postives = 249/267 (93.26%), Query Frame = 0


Query: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60
           MGKEDAK RSRTKYKGKTKFEEYLDMETG RNPGLSAE+DLELERKLAKKLKVKAGKLRG
Sbjct: 1   MGKEDAKLRSRTKYKGKTKFEEYLDMETGMRNPGLSAEQDLELERKLAKKLKVKAGKLRG 60

Query: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL 120
            DDGINVLFEGIPSIVDFPGEEV QFSEE AVEETKK PLGKKG +RKSLDQALD+ESEL
Sbjct: 61  EDDGINVLFEGIPSIVDFPGEEVSQFSEEFAVEETKKKPLGKKGKKRKSLDQALDIESEL 120

Query: 121 TTVVEEENASKKNKKRKKKK-RISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQ 180
           TTVVEEENASKKNKKRKKKK RISEKL+DVV E++AGDESMPVESHCVEAAVDKVP KAQ
Sbjct: 121 TTVVEEENASKKNKKRKKKKKRISEKLEDVVAEETAGDESMPVESHCVEAAVDKVPAKAQ 180

Query: 181 KYVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQII 240
           KYVAP LRLQKG EPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQII
Sbjct: 181 KYVAPNLRLQKGTEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQII 240

Query: 241 GDEILASCSRGPRGNEQLSVVLYFYLA 266
            DE+LASCSRGPRGNEQ + V   ++A
Sbjct: 241 SDEVLASCSRGPRGNEQYAAVFASFVA 267

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BLAST of Cucsat.G5368.T6 vs. NCBI nr
Match: XP_038882894.1 (nucleolar MIF4G domain-containing protein 1 [Benincasa hispida])

HSP 1 Score: 410 bits (1053), Expect = 4.66e-136
Identity = 226/274 (82.48%), Postives = 239/274 (87.23%), Query Frame = 0


Query: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60
           MGKEDAK RSRTK+KGKTKFEE+LDMETG RNPGLSAEEDLELERKLAKKLKVKAGKLRG
Sbjct: 1   MGKEDAKLRSRTKHKGKTKFEEFLDMETGMRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60

Query: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL 120
            DDGINVLFEGIPSIVDFPGEE LQFSEE AVE TK  PLGKKG +RKS DQ +D ESEL
Sbjct: 61  EDDGINVLFEGIPSIVDFPGEEELQFSEEFAVEATKNKPLGKKGKKRKSSDQTVDRESEL 120

Query: 121 TTV--------VEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVD 180
           T V        VEEE  SKKNKKRKKKK +SEKL DVVT+++AGDESMPVESHC EAAVD
Sbjct: 121 TVVDFEASEAVVEEEKISKKNKKRKKKK-MSEKLQDVVTDETAGDESMPVESHCAEAAVD 180

Query: 181 KVPPKAQKYVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISR 240
           KVP KAQKYVAPYLRLQK  EPEDHTQLRRRVRGLLNRLSESN+ESVTGEMSTVFHSISR
Sbjct: 181 KVPVKAQKYVAPYLRLQKRNEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVFHSISR 240

Query: 241 SIASQIIGDEILASCSRGPRGNEQLSVVLYFYLA 266
           SIASQII DE+LASCSRGPRGNEQ + V   ++A
Sbjct: 241 SIASQIISDEVLASCSRGPRGNEQYAAVFASFVA 273

[Close]

BLAST of Cucsat.G5368.T6 vs. NCBI nr
Match: XP_022149359.1 (nucleolar MIF4G domain-containing protein 1-like [Momordica charantia] >XP_022149368.1 nucleolar MIF4G domain-containing protein 1-like [Momordica charantia] >XP_022149374.1 nucleolar MIF4G domain-containing protein 1-like [Momordica charantia])

HSP 1 Score: 317 bits (811), Expect = 7.94e-100
Identity = 186/276 (67.39%), Postives = 212/276 (76.81%), Query Frame = 0


Query: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60
           MGKEDAK RSRTK+KGKTKFEEYL MET  RNPGLSAEEDLELERKLAKKLKVKAGKLRG
Sbjct: 1   MGKEDAKLRSRTKHKGKTKFEEYLGMETSMRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60

Query: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDME--- 120
            DDGINVLFEG+ S++D P EE L+FSEE AVE +KK  LGK G +RKS DQ  D +   
Sbjct: 61  EDDGINVLFEGMSSMIDSP-EEDLRFSEEFAVEPSKKQTLGKVGKKRKSSDQGPDGDFAS 120

Query: 121 ---SELTTVVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKV 180
              SE    VE+ + + K+KKRKKKK + +  DD+VT   AGD+S       +E AVDKV
Sbjct: 121 VEASEADIEVEKSSKASKSKKRKKKKMLEQHPDDIVTGGIAGDDS-------IEVAVDKV 180

Query: 181 PPKA----QKYVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSI 240
           P KA    QKYVAPYLRLQKG E ED TQLRRRVRGLLNRLSESN+ES+  EMST+F SI
Sbjct: 181 PVKALEGNQKYVAPYLRLQKGNESEDQTQLRRRVRGLLNRLSESNVESIIAEMSTIFRSI 240

Query: 241 SRSIASQIIGDEILASCSRGPRGNEQLSVVLYFYLA 266
           SRSIASQ+I +E+LASCSRGPRGNEQ + V   ++A
Sbjct: 241 SRSIASQVISEEVLASCSRGPRGNEQYAAVFASFVA 268

[Close]

BLAST of Cucsat.G5368.T6 vs. NCBI nr
Match: XP_022145796.1 (nucleolar MIF4G domain-containing protein 1-like [Momordica charantia])

HSP 1 Score: 311 bits (798), Expect = 8.09e-98
Identity = 185/283 (65.37%), Postives = 213/283 (75.27%), Query Frame = 0


Query: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60
           MGKEDAK RSRTK+KGKTKFEEYL MET  RN GLSAEEDLELERKLAKKLKVKAGKLRG
Sbjct: 1   MGKEDAKLRSRTKHKGKTKFEEYLGMETCMRNLGLSAEEDLELERKLAKKLKVKAGKLRG 60

Query: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL 120
            DDGINVLFEG+ S++D P EE L+FSEE AVE +KK  LG+ G +RKS D+  D ESEL
Sbjct: 61  EDDGINVLFEGMSSMIDSPEEE-LRFSEEFAVEPSKKKTLGQMGKKRKSSDEGPDGESEL 120

Query: 121 TT-------------VVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCV 180
           TT              VE+ + + K+K+R+KKK   +  DD+V  + AGD+S        
Sbjct: 121 TTGDFAPVGASEADIEVEKSSKAPKSKRRRKKKMSEQHQDDIVIGEIAGDDS-------T 180

Query: 181 EAAVDKVPPKA----QKYVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEM 240
           E AVDKVP KA    QKYVAPYLRLQKG E EDHTQLRRRVRGLLNRLSESN+ES+  EM
Sbjct: 181 EVAVDKVPVKASEGNQKYVAPYLRLQKGDESEDHTQLRRRVRGLLNRLSESNVESIIAEM 240

Query: 241 STVFHSISRSIASQIIGDEILASCSRGPRGNEQLSVVLYFYLA 266
           ST+F SISRSIASQ+I +E+LASCSRGPRGNEQ + V   ++A
Sbjct: 241 STIFRSISRSIASQVISEEVLASCSRGPRGNEQYAAVFASFVA 275

[Close]

BLAST of Cucsat.G5368.T6 vs. ExPASy TrEMBL
Match: A0A0A0KWL8 (MI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G188970 PE=4 SV=1)

HSP 1 Score: 486 bits (1252), Expect = 1.75e-166
Identity = 258/266 (96.99%), Postives = 261/266 (98.12%), Query Frame = 0


Query: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60
           MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG
Sbjct: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60

Query: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL 120
           VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL
Sbjct: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL 120

Query: 121 TTVVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQK 180
           TTVVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQK
Sbjct: 121 TTVVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQK 180

Query: 181 YVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQIIG 240
           YVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQIIG
Sbjct: 181 YVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQIIG 240

Query: 241 DEILASCSRGPRGNEQLSVVLYFYLA 266
           DEILASCSRGPRGNEQ + V   ++A
Sbjct: 241 DEILASCSRGPRGNEQYAAVFASFVA 266

[Close]

BLAST of Cucsat.G5368.T6 vs. ExPASy TrEMBL
Match: A0A5D3CAM3 (Nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00080 PE=4 SV=1)

HSP 1 Score: 441 bits (1134), Expect = 1.19e-148
Identity = 239/267 (89.51%), Postives = 249/267 (93.26%), Query Frame = 0


Query: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60
           MGKEDAK RSRTKYKGKTKFEEYLDMETG RNPGLSAE+DLELERKLAKKLKVKAGKLRG
Sbjct: 1   MGKEDAKLRSRTKYKGKTKFEEYLDMETGMRNPGLSAEQDLELERKLAKKLKVKAGKLRG 60

Query: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL 120
            DDGINVLFEGIPSIVDFPGEEV QFSEE AVEETKK PLGKKG +RKSLDQALD+ESEL
Sbjct: 61  EDDGINVLFEGIPSIVDFPGEEVSQFSEEFAVEETKKKPLGKKGKKRKSLDQALDIESEL 120

Query: 121 TTVVEEENASKKNKKRKKKK-RISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQ 180
           TTVVEEENASKKNKKRKKKK RISEKL+DVV E++AGDESMPVESHCVEAAVDKVP KAQ
Sbjct: 121 TTVVEEENASKKNKKRKKKKKRISEKLEDVVAEETAGDESMPVESHCVEAAVDKVPAKAQ 180

Query: 181 KYVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQII 240
           KYVAP LRLQKG EPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQII
Sbjct: 181 KYVAPNLRLQKGTEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQII 240

Query: 241 GDEILASCSRGPRGNEQLSVVLYFYLA 266
            DE+LASCSRGPRGNEQ + V   ++A
Sbjct: 241 SDEVLASCSRGPRGNEQYAAVFASFVA 267

[Close]

BLAST of Cucsat.G5368.T6 vs. ExPASy TrEMBL
Match: A0A1S3B470 (nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo OX=3656 GN=LOC103485994 PE=4 SV=1)

HSP 1 Score: 441 bits (1134), Expect = 1.19e-148
Identity = 239/267 (89.51%), Postives = 249/267 (93.26%), Query Frame = 0


Query: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60
           MGKEDAK RSRTKYKGKTKFEEYLDMETG RNPGLSAE+DLELERKLAKKLKVKAGKLRG
Sbjct: 1   MGKEDAKLRSRTKYKGKTKFEEYLDMETGMRNPGLSAEQDLELERKLAKKLKVKAGKLRG 60

Query: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL 120
            DDGINVLFEGIPSIVDFPGEEV QFSEE AVEETKK PLGKKG +RKSLDQALD+ESEL
Sbjct: 61  EDDGINVLFEGIPSIVDFPGEEVSQFSEEFAVEETKKKPLGKKGKKRKSLDQALDIESEL 120

Query: 121 TTVVEEENASKKNKKRKKKK-RISEKLDDVVTEDSAGDESMPVESHCVEAAVDKVPPKAQ 180
           TTVVEEENASKKNKKRKKKK RISEKL+DVV E++AGDESMPVESHCVEAAVDKVP KAQ
Sbjct: 121 TTVVEEENASKKNKKRKKKKKRISEKLEDVVAEETAGDESMPVESHCVEAAVDKVPAKAQ 180

Query: 181 KYVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQII 240
           KYVAP LRLQKG EPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQII
Sbjct: 181 KYVAPNLRLQKGTEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQII 240

Query: 241 GDEILASCSRGPRGNEQLSVVLYFYLA 266
            DE+LASCSRGPRGNEQ + V   ++A
Sbjct: 241 SDEVLASCSRGPRGNEQYAAVFASFVA 267

[Close]

BLAST of Cucsat.G5368.T6 vs. ExPASy TrEMBL
Match: A0A6J1D6L2 (nucleolar MIF4G domain-containing protein 1-like OS=Momordica charantia OX=3673 GN=LOC111017795 PE=4 SV=1)

HSP 1 Score: 317 bits (811), Expect = 3.85e-100
Identity = 186/276 (67.39%), Postives = 212/276 (76.81%), Query Frame = 0


Query: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60
           MGKEDAK RSRTK+KGKTKFEEYL MET  RNPGLSAEEDLELERKLAKKLKVKAGKLRG
Sbjct: 1   MGKEDAKLRSRTKHKGKTKFEEYLGMETSMRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60

Query: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDME--- 120
            DDGINVLFEG+ S++D P EE L+FSEE AVE +KK  LGK G +RKS DQ  D +   
Sbjct: 61  EDDGINVLFEGMSSMIDSP-EEDLRFSEEFAVEPSKKQTLGKVGKKRKSSDQGPDGDFAS 120

Query: 121 ---SELTTVVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKV 180
              SE    VE+ + + K+KKRKKKK + +  DD+VT   AGD+S       +E AVDKV
Sbjct: 121 VEASEADIEVEKSSKASKSKKRKKKKMLEQHPDDIVTGGIAGDDS-------IEVAVDKV 180

Query: 181 PPKA----QKYVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSI 240
           P KA    QKYVAPYLRLQKG E ED TQLRRRVRGLLNRLSESN+ES+  EMST+F SI
Sbjct: 181 PVKALEGNQKYVAPYLRLQKGNESEDQTQLRRRVRGLLNRLSESNVESIIAEMSTIFRSI 240

Query: 241 SRSIASQIIGDEILASCSRGPRGNEQLSVVLYFYLA 266
           SRSIASQ+I +E+LASCSRGPRGNEQ + V   ++A
Sbjct: 241 SRSIASQVISEEVLASCSRGPRGNEQYAAVFASFVA 268

[Close]

BLAST of Cucsat.G5368.T6 vs. ExPASy TrEMBL
Match: A0A6J1CXG6 (nucleolar MIF4G domain-containing protein 1-like OS=Momordica charantia OX=3673 GN=LOC111015171 PE=4 SV=1)

HSP 1 Score: 311 bits (798), Expect = 3.91e-98
Identity = 185/283 (65.37%), Postives = 213/283 (75.27%), Query Frame = 0


Query: 1   MGKEDAKHRSRTKYKGKTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRG 60
           MGKEDAK RSRTK+KGKTKFEEYL MET  RN GLSAEEDLELERKLAKKLKVKAGKLRG
Sbjct: 1   MGKEDAKLRSRTKHKGKTKFEEYLGMETCMRNLGLSAEEDLELERKLAKKLKVKAGKLRG 60

Query: 61  VDDGINVLFEGIPSIVDFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQALDMESEL 120
            DDGINVLFEG+ S++D P EE L+FSEE AVE +KK  LG+ G +RKS D+  D ESEL
Sbjct: 61  EDDGINVLFEGMSSMIDSPEEE-LRFSEEFAVEPSKKKTLGQMGKKRKSSDEGPDGESEL 120

Query: 121 TT-------------VVEEENASKKNKKRKKKKRISEKLDDVVTEDSAGDESMPVESHCV 180
           TT              VE+ + + K+K+R+KKK   +  DD+V  + AGD+S        
Sbjct: 121 TTGDFAPVGASEADIEVEKSSKAPKSKRRRKKKMSEQHQDDIVIGEIAGDDS-------T 180

Query: 181 EAAVDKVPPKA----QKYVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEM 240
           E AVDKVP KA    QKYVAPYLRLQKG E EDHTQLRRRVRGLLNRLSESN+ES+  EM
Sbjct: 181 EVAVDKVPVKASEGNQKYVAPYLRLQKGDESEDHTQLRRRVRGLLNRLSESNVESIIAEM 240

Query: 241 STVFHSISRSIASQIIGDEILASCSRGPRGNEQLSVVLYFYLA 266
           ST+F SISRSIASQ+I +E+LASCSRGPRGNEQ + V   ++A
Sbjct: 241 STIFRSISRSIASQVISEEVLASCSRGPRGNEQYAAVFASFVA 275

[Close]

BLAST of Cucsat.G5368.T6 vs. TAIR 10
Match: AT5G17930.1 (MIF4G domain-containing protein / MA3 domain-containing protein )

HSP 1 Score: 160.6 bits (405), Expect = 1.8e-39
Identity = 104/271 (38.38%), Postives = 169/271 (62.36%), Query Frame = 0


Query: 17  KTKFEEYLDMETGTRNPGLSAEEDLELERKLAKKLKVKAGKLRGVDDGINVLFEGIPSIV 76
           KT FEE+L+M+T T    +S ++D ELER+LAKKLK+K GKLRG+DDG+N LFEG+PS++
Sbjct: 111 KTNFEEFLEMDTPT---VISGDQDAELERRLAKKLKIKKGKLRGMDDGLNDLFEGLPSVL 170

Query: 77  DFPGEEVLQFSEECAVEETKKNPLGKKGNRRKSLDQAL--DMESELTTV----VEEENAS 136
           D  G E+    ++      +K    ++    + +D+    D+E E +       EEE   
Sbjct: 171 DSMGSELGDSRKK------RKKKRSEEKQDHEDVDELANEDLEHEESEFSDEESEEEPVG 230

Query: 137 KKNKKR--KKKKRISEKLDDVVTEDSAGDESMPVESHCVEAAVDKV--------PPKAQK 196
           K+++KR  KKKK + E+L+  +   +   ES  V  H   ++++KV        P  + K
Sbjct: 231 KRDRKRHKKKKKSVDEELESDLMNITDDGESETVNYHDSPSSLEKVDTPLHERKPESSSK 290

Query: 197 YVAPYLRLQKGAEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQIIG 256
           YVAP+LR Q  +E E+ T++R R++GLLN+++ESN+E++T E++T++ S++RS++SQI  
Sbjct: 291 YVAPHLRSQAKSESEELTKMRTRIKGLLNKMAESNVETITAELATIYRSVARSVSSQIFC 350

Query: 257 DEILASCSRGPRGNEQLSVVLYFYLALLSIV 272
           +E+L + +   RGNEQ +V   F   +  +V
Sbjct: 351 EEVLTTYA---RGNEQYAVFASFIAGMACLV 369

The following BLAST results are available for this feature:

BLAST of Cucsat.G5368.T6 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5C9Z4	6.9e-04	24.90	Nucleolar MIF4G domain-containing protein 1 OS=Homo sapiens OX=9606 GN=NOM1 PE=1...	[more]

BLAST of Cucsat.G5368.T6 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149114.1	3.61e-166	96.99	nucleolar MIF4G domain-containing protein 1 [Cucumis sativus] >KGN53923.1 hypoth...	[more]
XP_008441997.1	2.45e-148	89.51	PREDICTED: nucleolar MIF4G domain-containing protein 1 [Cucumis melo] >KAA004172...	[more]
XP_038882894.1	4.66e-136	82.48	nucleolar MIF4G domain-containing protein 1 [Benincasa hispida]	[more]
XP_022149359.1	7.94e-100	67.39	nucleolar MIF4G domain-containing protein 1-like [Momordica charantia] >XP_02214...	[more]
XP_022145796.1	8.09e-98	65.37	nucleolar MIF4G domain-containing protein 1-like [Momordica charantia]	[more]

BLAST of Cucsat.G5368.T6 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWL8	1.75e-166	96.99	MI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G188970 PE=4 SV=...	[more]
A0A5D3CAM3	1.19e-148	89.51	Nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B470	1.19e-148	89.51	nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]
A0A6J1D6L2	3.85e-100	67.39	nucleolar MIF4G domain-containing protein 1-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1CXG6	3.91e-98	65.37	nucleolar MIF4G domain-containing protein 1-like OS=Momordica charantia OX=3673 ...	[more]

BLAST of Cucsat.G5368.T6 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17930.1	1.8e-39	38.38	MIF4G domain-containing protein / MA3 domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016021	MIF4G-like domain superfamily	GENE3D	1.25.40.180		coord: 168..272 e-value: 6.0E-12 score: 47.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 122..161
None	No IPR available	PANTHER	PTHR18034:SF4	NUCLEOLAR MIF4G DOMAIN-CONTAINING PROTEIN 1	coord: 6..269
None	No IPR available	PANTHER	PTHR18034	CELL CYCLE CONTROL PROTEIN CWF22-RELATED	coord: 6..269

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G5368	Cucsat.G5368	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G5368.T6.E1	Cucsat.G5368.T6.E1	exon
Cucsat.G5368.T6.E2	Cucsat.G5368.T6.E2	exon
Cucsat.G5368.T6.E3	Cucsat.G5368.T6.E3	exon
Cucsat.G5368.T6.E4	Cucsat.G5368.T6.E4	exon
Cucsat.G5368.T6.E5	Cucsat.G5368.T6.E5	exon
Cucsat.G5368.T6.E6	Cucsat.G5368.T6.E6	exon
Cucsat.G5368.T6.E7	Cucsat.G5368.T6.E7	exon
Cucsat.G5368.T6.E8	Cucsat.G5368.T6.E8	exon
Cucsat.G5368.T6.E9	Cucsat.G5368.T6.E9	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G5368.T6.C3	Cucsat.G5368.T6.C3	CDS
Cucsat.G5368.T6.C2	Cucsat.G5368.T6.C2	CDS
Cucsat.G5368.T6.C1	Cucsat.G5368.T6.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G5368.T6	Cucsat.G5368.T6-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006417	regulation of translation
biological_process	GO:0042274	ribosomal small subunit biogenesis
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003723	RNA binding