Cucsat.G5368.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G5368.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	nucleolar MIF4G domain-containing protein 1
Location	ctg1267: 280509 .. 285777 (-)
RNA-Seq Expression	Cucsat.G5368.T4
Synteny	Cucsat.G5368.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAAATTGGATGGAAAATAGATGGACAACGAGCGGGAGGGCACTGTTAGTCTAAATCTCAATTCGGCCTTCGGAAGAGTCATCCCCTTCTCCGCAAAAACCCCTTTCCTAAACCCCTCCACCCACCGGGCACCAGTCACCACCCTCTTCATCACCCTTCTTCTCTATCCCCCTTACTCACCGACGCCGCTTCTCTCTCTCTCTCTCTCTCTCTCTCTTTTCATCTTTTTTCTTCACCTCAAAATTGGTTTCATTTGCCTTTTCAAATACCCCAATTTCATAACTGAAACTGTGTCTTCGCCGGTAGTGTAAACAGGAAGCAAGGGGGAATGGGGAAGGAGGATGCGAAGCACCGGTCTCGGACCAAGTACAAGGGAAAAACGAAATTCGAGGAGTATTTGGATATGGAAACGGGCACGCGAAACCCTGGGTTATCTGCTGAGGAGGACTTGGAATTGGAACGAAAGCTTGCAAAGAAATTGAAGGTCAAGGCTGGGAAGTTAAGGGGAGTGGATGATGGTATCAATGTGTTATTTGAAGGAATCCCTTCCATTGTTGATTTCCCTGGGGAGGAAGTATTGCAGTTTTCTGAGGAGTGCGCTGTTGAGGAAACTAAGAAAAATCCATTAGGTAAAAAGGGGAACAGAAGGAAATCATTAGATCAAGCCCTAGATATGGAGAGTGAGTTAACTACTGTTGTTGAGGAGGAAAATGCTTCAAAGAAAAATAAGAAACGTAAGAAGAAGAAGAGGATATCTGAAAAACTTGACGATGTAGTGACAGAGGATTCCGCTGGTGATGAATCTATGCCTGTGGAATCACATTGTGTAGAGGCTGCAGTGGATAAAGTTCCTCCCAAAGCTCAAAAATATGTTGCTCCTTACTTGAGATTGCAAAAAGGAGCTGAGCCTGAAGATCATACTCAACTCCGTCGGCGTGTTCGAGGTGTGGAGGTTGTATGATTATGCGTTCATTTTTTTCCCCTTTCTTGTTTTTTAATTTGGATGTAATTTGCGTTGTATTGATTTTTCATCAGGTCTTCTAAATAGGCTCTCTGAATCAAATATCGAATCGGTTACAGGGGAAATGTCTACGGTTTTTCATGTATGATGTTATCTTCTAACTATCTCGTTACCTATGTTTCTTGAATGTCTATTACAGCCTTACTGAGCGCTTGTAACTTGTGATCAATGGTATCTAGTCTCTATTTGATCTAAGCTCGTCAGTGAATCTATTGTGTTGTATCAATTTGCAGTCTATTTCTCGCAGCATTGCTTCTCAAATTATTGGTGATGAGATTCTGGCATCTTGTTCACGTGGTCCTCGAGGCAATGAACAGTTAAGTGTTGTTTTATATTTTTATTTGGCACTTCTATCTATTGTTGTTTATATATGTTGAATCCTTTTCTTTTTTAAAATATATAGTTAGATTTTATTTTGATAAAAGTTTTATATTTAACTTCTCATTCATCACTTCACTATAACTTTTTTATTATAGTTAAGAACATTGGTTGACACAATGTTGAGGAGTTTCTGCTGAATTGGTCAAATTTGAAACTTTGTATAATGATAACATGTTGACCGTTTGTTTTTTGGGTTTCTCTAACTTTTGACAAACTCCTTTGTTTGTTTGTTTCTTCTTGTTCAAGGTATGCTGCTGTATTTGCCTCTTTTGTTGCAGGCATGGGTTGTTTGGTTGGTACTGACTTTAGCGCAAGGCTCATGGCTTCCCTTGCTAAAACATTTGAGGTGAGCTTGCCTGCTAAATACATCATTTAATTATTTGGTTTTGCATGGCTGTAGAGACCTCTACTAAAGTGATAAATACTTTTTTTTCAGGATGAATACCTCAATGAAGATAATCTTTCTTTGCGGAACCTTACACTATTGCTTTCCTATTTATGTGTATTTGGGGTTTGCGCCAGGTAAGTACATATATCCACACATTATGTATTATTCACGATTTTTTTTTCTTTTTGTTTGTTTTTCAAATATCAGATAATTGTGTGTTTTAGTTTGTATAATCTATTTCATTATACTGGATGCATGTGATAGACCTCCCATCATATACCAATATAATAGAAGAAAGAAAGTTAGTTAGTAGACTGTTAGCCAATACAGTTCGTCAGGAAGTTAAGAAGTTAGTTAACAGTTAGTCAGGAAGTTAAGAAGTTAGCTGGTACCGTTAGTGCTATGGTAATAAAAAGAGAGGCTAAGAAAGAAAGAGGCAGGAAAATTATAGTGAGTAAATAGAGAGAGACTTCTGTGCATCTTTGAAAGACAAGATACAGAAGAGCAGAATTCCCTACATGTAAAGTCATTGACTAGATACTATTTTAAAAGATTACTTCATAATACATACGGTTCCCATCAGCGTGCACGTCTTTCTAATAAGCATTTTTTCTAAATACTTTTCAGTGATCTGATCTATGATTTTCTAATTATACTGAGCAAGCGGTTGACAGAAATTGATGTATCCACCATCCTGACAGTTTTACAATGTAAGTTGATTTCAAGCTTGATATGTTTAGATATAGCACCATCTCCTTGTTAATCCACTACTAGAGTTTCTCTTTAAATTGGAATTTTGTCTTGTTTAAATGTCAAGATGTTAAATATATTCTAAATTCATTATCCCTTTTTCTAATCGTTCCTAGATATTTCCTGAACATTAGCAGACTCAGGGCTGTGCATTATGTTAAAAGTTGTGCTTGGATTTAACCACCGGTGATGTTTCTTTTTCAAAAAAAAAAAAGAAAAGATCGAACCACTTGTGATGGATATTTAGTAGTTCATAAATTTTGTCAATAACAAAGGCTGCTGACTTAGATGGTTGTTCACTTTCAAATATTGAATTTGACTATTATATCGTTGATGGATGTTCATATCATGGGTTGAATATTCTTAGCTTCTCAATTCTTTTATATTCTAACAAAATTATTGGTCTTGAACTCTTAATATATTACAACAAATTGATGGTCATGGGAAGTTGATATATAACTTAAATCGTCTCTAATTTGGTAGGTTGTGGAATGAAAATAAGGGCTGATGATCCCACTGCAATGAAGAGTTTTATTGTCAGTGTTCAAAGTAAGGTTAATGAGCTAAAGGCAGCTTCTGGAGATGGTCTACAAAATATCAATGGGAAGAGAGTGAGTCTCTATAAGTAGTATTTCATTTGCTGAGTTTTTAAATTTTTAAAATTTTGATTTTCTAATATTACAACGTGGATGCATGCAGATGGAGTTCATGCTGGAGACTATATGTGACATCAAGAATAACAAGAAAAGATCTAAGGATGATCCAGCCCACCATACAAGGATAAAGAAATGGCTACAGAAGGTCTACCTTATTCTATTTCATTGGCTAGCATTGCATGTTTGTGGTTACATTATTTTGTAGTGCGTAGATCTTAATTTGCTAGATAATTGCTCTTTATAGAGTAAACATTTTACTTCAAAAGCAGACTTGTTCACTTGAAAGGTCAAGTTGGATAATTTTTTGTAGGTCTAATGCAAGGACAGTGAAAGATTTTCAATTAGTCTCTTTTTGAGCGCATGAAACATTTTTAATATCTTAGTCAAGTAGTAACCATCATGGGTTGACCTAGTCGTAAAAAGGTCAATAACTAAGAGGTCATGGCATGGTGGCCACCTACCTAGGAATTAATTTCTTCTGGGTTTTCTTGACACCCAAATGTTGTAGGGTCAGGTGGGTTGTCCCGTGAGATTAGTTGAAGTGTGCGTAAGCAGGCTTGGACACTCACGGATATGAAAAAAAAATCTTAGTCAAGTAGTCATGATGACTTTTATTCTCCCAAAAATGTTTCTTACTTTTTGCATGTTGTAATGCTATTAGGCCAACTCATCGATAGTTAAACAAAAAGTGGGATTCTATGCTTGAACTACCTAACCTTGCATTGTTGAGTGTGATTGAAATCTGACCTTTTTTTAAGCCTCATTCTTCAATTATTATTTTCAGTTAGGAGTAGATGATATAATAATTAGAGGGATCAAATGGAACAAGTTGCTTGATCCTGACAAGAAGGGGCAGTGGTGGTTGTCTGGGGATTTGTCAACGACATCAGATAACGTTGAAGAGTTTGCCAACACTATTGATAAAGAGGTACTTGAAGCTCAAAAAATGCTTCAGCTTGCAGCTGCACAAAGAATGAATACAGATGCTAGGAAAGCGATATTTTGTATTATAATGAGTGGAGAGGACTACGTTGATGCATTTGAGAAGCTAATTAGGTTGGATTTATCTGGAAAGCAGGTTGGTGGAATTTTGTAAATTTTCTTCACCCTACCTCCAAATCATACCTCTTATTGTGCTTACCATCTCCATGTCGTCTACAGGACCGGGAGATCATGAGGGTTCTTGTGGACTGTTGTTTACAAGAGAAAGTGTTTAACAAGTACTACACTGTTCTAGCTTCCAAATTGTGTGAGCATGAAAAGAACCACAAGTTTACATTACAGGTAAGTTCTTTGCCTCTTTGGTCTGTACTCAGTTTGGTTAATGAAACTTTAGATACGGTTGCGAAAGTACCATCTTCTAACTACGCCACACCTCATCTTCACAGTATTGCTTGTGGGATCAATTCAAAGAACTGGATACGATGCAACTTATCAGGTCGATGAATCTTGCGAAGTTTGTCGCCGAGATGATCACCTCTTTTACTCTCTCACTTGCTGTCTTGAAGTCAGTAGATTTGAGTGATATTAGGCTGCTAACTGCAAAGAGAATCATGCATTTTCGTATGCTGTTTGATGCAATTTTTGAACGTCCTGACAAGCTGATATGGAACGTGTTCACTCGAGTTGCTGTTAACCCTGAGCTCGAACCTCTTCGAAGTGGTATGTTGTTCTTCATTAAAGAGTACATGATTAAAACTAGTAAAGTTAATGCAGAGAAGTTTAAGCTTATCAAGAAAGCGCTTAACAATGTTGAGGGAATACTAATGTGAATTTTGGAGTGAGAATTAGAGTTTTCAAAGTTCTCTTTTTGATCTTACTTATAGAGAAGAGGGATGTGTTGTACACTGTCATGTTTTGGCTAAATGAAAGAATAAACTGCTATTTTGTAACTCAAATTATTTCTTGAAGGTTTTAAATCTTGGAGTAAATTACTCATACGGGTTTGATACTTATTTTTCTTTGTTAAGATTTTTGTATTTTTGTAATTTCTTTCCCGTACTTTGCATTCTTATCTGGTAGGTATTTATCAAAATAAAAATGAGAAATGAGAATAAAATATGAAGTCTCAAT

Coding sequence (CDS)

ATGAAAATAAGGGCTGATGATCCCACTGCAATGAAGAGTTTTATTGTCAGTGTTCAAAGTAAGGTTAATGAGCTAAAGGCAGCTTCTGGAGATGGTCTACAAAATATCAATGGGAAGAGAATGGAGTTCATGCTGGAGACTATATGTGACATCAAGAATAACAAGAAAAGATCTAAGGATGATCCAGCCCACCATACAAGGATAAAGAAATGGCTACAGAAGTTAGGAGTAGATGATATAATAATTAGAGGGATCAAATGGAACAAGTTGCTTGATCCTGACAAGAAGGGGCAGTGGTGGTTGTCTGGGGATTTGTCAACGACATCAGATAACGTTGAAGAGTTTGCCAACACTATTGATAAAGAGGTACTTGAAGCTCAAAAAATGCTTCAGCTTGCAGCTGCACAAAGAATGAATACAGATGCTAGGAAAGCGATATTTTGTATTATAATGAGTGGAGAGGACTACGTTGATGCATTTGAGAAGCTAATTAGGTTGGATTTATCTGGAAAGCAGGACCGGGAGATCATGAGGGTTCTTGTGGACTGTTGTTTACAAGAGAAAGTGTTTAACAAGTACTACACTGTTCTAGCTTCCAAATTGTGTGAGCATGAAAAGAACCACAAGTTTACATTACAGTATTGCTTGTGGGATCAATTCAAAGAACTGGATACGATGCAACTTATCAGGTCGATGAATCTTGCGAAGTTTGTCGCCGAGATGATCACCTCTTTTACTCTCTCACTTGCTGTCTTGAAGTCAGTAGATTTGAGTGATATTAGGCTGCTAACTGCAAAGAGAATCATGCATTTTCGTATGCTGTTTGATGCAATTTTTGAACGTCCTGACAAGCTGATATGGAACGTGTTCACTCGAGTTGCTGTTAACCCTGAGCTCGAACCTCTTCGAAGTGGTATGTTGTTCTTCATTAAAGAGTACATGATTAAAACTAGTAAAGTTAATGCAGAGAAGTTTAAGCTTATCAAGAAAGCGCTTAACAATGTTGAGGGAATACTAATGTGA

Protein sequence

MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKDDPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTIDKEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVLVDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAEMITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELEPLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM

Homology

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BLAST of Cucsat.G5368.T4 vs. ExPASy Swiss-Prot
Match: Q5C9Z4 (Nucleolar MIF4G domain-containing protein 1 OS=Homo sapiens OX=9606 GN=NOM1 PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.6e-50
Identity = 117/329 (35.56%), Postives = 194/329 (58.97%), Query Frame = 0


Query: 3   IRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD-- 62
           +R DD  ++K  I   Q+K      ASG G +  +  R+ FMLET+  +KNN  R     
Sbjct: 513 LRKDDALSLKELITEAQTK------ASGAGSEFQDQTRIRFMLETMLALKNNDMRKIPGY 572

Query: 63  DPAHHTRIKKWLQKL----GVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFA 122
           DP    +++K  + L    G        + W+ +L  ++ G+WW+ G   + +  ++   
Sbjct: 573 DPEPVEKLRKLQRALVRNAGSGSETQLRVSWDSVLSAEQTGRWWIVGSAWSGAPMIDNSH 632

Query: 123 NT-IDKEVL--EAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQD 182
           +T + K+++   + K+L+LA  QRMNTD R+ IFC IM+ ED++DAFEKL++L L  +Q+
Sbjct: 633 HTHLQKQLVGTVSSKILELARKQRMNTDIRRNIFCTIMTSEDFLDAFEKLLKLGLKDQQE 692

Query: 183 REIMRVLVDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMN 242
           REI+ VL+DCCLQEK +N +Y  LASK CE+E+  + T Q+ +WD+F++L+ +      N
Sbjct: 693 REIIHVLMDCCLQEKTYNPFYAFLASKFCEYERRFQMTFQFSIWDKFRDLENLPATNFSN 752

Query: 243 LAKFVAEMITSFTLSLAVLKSVDLSDIRLLTAKRIMHFR-MLFDAIFERPDKLIWNVFTR 302
           L   VA ++ + +LSL++LK V+ S+   L   R+   R +L   + E   + +  +FTR
Sbjct: 753 LVHLVAHLLKTKSLSLSILKVVEFSE---LDKPRVRFLRKVLSILLMETEVEDLSLIFTR 812

Query: 303 VAVNPELEPLRSGMLFFIKEYMIKTSKVN 322
           V+ NP+L  LR G+  FI  +++K ++ +
Sbjct: 813 VSDNPKLGVLREGLKLFISHFLLKNAQAH 832

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BLAST of Cucsat.G5368.T4 vs. ExPASy Swiss-Prot
Match: Q3UFM5 (Nucleolar MIF4G domain-containing protein 1 OS=Mus musculus OX=10090 GN=Nom1 PE=1 SV=2)

HSP 1 Score: 194.5 bits (493), Expect = 1.9e-48
Identity = 118/341 (34.60%), Postives = 198/341 (58.06%), Query Frame = 0


Query: 3   IRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNN--KKRSKD 62
           +R DD  ++K  I   Q++      ASG G +  +  R+ FMLET+  +KNN  +K    
Sbjct: 507 LRKDDALSLKELITEAQTQ------ASGAGNKFQDQNRVRFMLETMLALKNNDLRKIPGY 566

Query: 63  DPAHHTRIKKWLQKL----GVDDIIIRGIKWNKLLDPDKKGQWWLSGDL---STTSDNVE 122
           +P    ++KK  + L    G        I W+ +L+ ++ G+WW+ G     +   DN  
Sbjct: 567 NPEPVEKLKKLQRTLVRNAGSGSETRLRISWDGILNAEQTGRWWIVGSAWSGTPMIDNSH 626

Query: 123 EFANTIDKEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQD 182
                     + + KML+LA  QRMNTD R+ IFC +M+ ED++DAFEKL++L L  +Q+
Sbjct: 627 HIQLQKPLAGMASSKMLELARKQRMNTDVRRIIFCTLMTSEDFLDAFEKLLKLGLKDQQE 686

Query: 183 REIMRVLVDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMN 242
           REI+ +L+DCCLQEK +N +Y  LASK C++E+  + T Q+ +WD+F++L+ +   +  N
Sbjct: 687 REIVHILMDCCLQEKTYNPFYAFLASKFCDYERRFQMTFQFSIWDKFRDLENLPDTKFSN 746

Query: 243 LAKFVAEMITSFTLSLAVLKSVDLSDIRLLTAKRIMHF--RMLFDAIFERPDKLIWNVFT 302
           L   +A ++ + +L L+VLK V+ S++     K  +HF  R+L   + E  D  +  +F+
Sbjct: 747 LVHLLAHLLRTKSLPLSVLKVVEFSEL----DKPRVHFLRRVLTALLMETEDDDLAVIFS 806

Query: 303 RVAVNPELEPLRSGMLFFIKEYMIKTSKV--NAEKFKLIKK 331
           RV+ NP+L  LR G+  FI  +++K ++   +AE+  L+++
Sbjct: 807 RVSDNPKLGMLREGLKLFIGHFLLKHTQAHQSAEEASLLRE 837

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BLAST of Cucsat.G5368.T4 vs. ExPASy Swiss-Prot
Match: Q9W020 (Nucleolar MIF4G domain-containing protein 1 homolog OS=Drosophila melanogaster OX=7227 GN=CG9004 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.8e-37
Identity = 103/356 (28.93%), Postives = 189/356 (53.09%), Query Frame = 0


Query: 2   KIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNN--KKRSK 61
           ++R DDP A K+ +  VQS++      +   L+     R+ FM++ +  +KNN  +K  +
Sbjct: 506 RLRKDDPLAFKTMMQKVQSQI------ASAPLELKENPRLRFMVDILNAVKNNNMQKLPQ 565

Query: 62  DDPAHHTRIKKWLQKLGVDD--IIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFAN 121
            DP     ++K L+ +  +D  ++   I    LL  DK G+WW+ G  S  + N++E  +
Sbjct: 566 YDPELAENLRKRLKAMLKNDRYVVTLNITLEDLLRADKVGKWWIVG--SAWTGNLDEMGS 625

Query: 122 TIDKEVLEAQK---------MLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDL 181
              K+   + K         +L+LA  Q+MNT  R+ IFCIIMS  DYVDAFEK++ L L
Sbjct: 626 AKQKQDKNSSKSANGGFADQLLELARKQQMNTAERRNIFCIIMSAADYVDAFEKILHLSL 685

Query: 182 SGKQDREIMRVLVDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQL 241
             K  R +  V++ C L EK  N YY  LA K C+  + ++   Q+  WD+  +++ +  
Sbjct: 686 --KDQRAVAYVIIHCALNEKRANPYYAHLALKFCQFNRKYQLAFQFASWDRINDIEKLSK 745

Query: 242 IRSMNLAKFVAEMITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWN 301
            +  NLA F+ ++I +  L L+VLK VD   +  L+   +    ++   +    ++ I+ 
Sbjct: 746 PQIRNLASFLQQVILAAGLQLSVLKVVDFMQLDKLSFYFMK--EVMVRLLLANDEREIYQ 805

Query: 302 VFTRVAVNPELEPLRSGMLFFIKEYMIKTSKVNAEKFK-----LIKKALNNVEGIL 340
            F RVA N +L+  +  +  F++ ++++  +++  K K     L+++ +++++ +L
Sbjct: 806 TFERVAKNTKLQQFKQSVRLFLQHFLLQEEQLDKLKLKDEDRQLLQQRVDHIDKLL 849

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BLAST of Cucsat.G5368.T4 vs. ExPASy Swiss-Prot
Match: O13971 (Suppressor of glycerol defect protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sgd1 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.2e-27
Identity = 106/368 (28.80%), Postives = 181/368 (49.18%), Query Frame = 0


Query: 2   KIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKDD 61
           ++R+DDPT+++  +    +++N L A++     ++   R +FM+E+I ++K NKK    +
Sbjct: 417 QLRSDDPTSLQDIV----TEMNLLLASADPSTISV---RTKFMVESITNLKENKKTKVAN 476

Query: 62  PAHHTR------IKKWLQKLGVDDIIIR---GIKWNKLLDPDKKGQWWLSGDLSTTSDNV 121
            +  ++      +KK+L  LG   +  R    +    +   + KG+WWL G    + +NV
Sbjct: 477 ASAQSKFEAVNQLKKFLGSLGNRSLNAREPLRVTLEDIEQIETKGRWWLVG---ASWNNV 536

Query: 122 EEFANTIDKEVLEAQ----------KMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEK 181
               NT+  E L+ +          K+LQ A   R+N+  R +IF  ++  EDY+DA+E+
Sbjct: 537 PSGDNTLSTEALQDKKKSEELTAHSKILQAAKKLRLNSTLRTSIFVALVGSEDYIDAWER 596

Query: 182 LIRLDLSGKQDREIMRVLVDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKE 241
           +++L L   Q  EI  V++ C   EK++N +Y ++A K C  + N K + Q+ LWD F E
Sbjct: 597 VLKLHLKRNQLPEIAYVILHCVGNEKLYNPFYGLVALKCCTLQHNLKKSFQFSLWDFFNE 656

Query: 242 LD--------TMQLIRSMNLAKFVAEMITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRML 301
           L          + + R +NLAK  A ++      L +LK VD   I      ++  F ++
Sbjct: 657 LQPDDDSEEREISMRRIVNLAKLYASLVIEAAQPLTILKHVDFMAIN----AQMQTFLLV 716

Query: 302 F--DAIFERPDKLIWNVFTRVAVNPELEP-LRSGMLFFIKEYMIKTSKVNAEKFKLIKKA 340
           F  D I    D L      ++  N + E  L S + +F+K Y+ K   V+  K  L K  
Sbjct: 717 FFTDIILGVKDDL---QLVKIFENCKAEKNLSSKVDWFLKTYVRKNPLVDNSKKALFKSN 767

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BLAST of Cucsat.G5368.T4 vs. ExPASy Swiss-Prot
Match: Q06132 (Suppressor of glycerol defect protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SGD1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.2e-23
Identity = 88/297 (29.63%), Postives = 132/297 (44.44%), Query Frame = 0


Query: 3   IRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKDDP 62
           IR DDP+A++     +      LK  S          R+ F+++T+ D+KNN+ +     
Sbjct: 497 IRGDDPSALRDIRSELLKNAKNLKEQS---------PRLRFLMDTMSDLKNNRLKPSILA 556

Query: 63  AHHTRIKKWLQKLGVDDIIIRGIKWNKL-------LDPDKKGQWWLSG--------DLST 122
             H  +KK LQ      I+     W  L        + D KG+WWL G        +   
Sbjct: 557 TDHHPLKKNLQ-----SILNSSSSWEPLQVSLEDIKNIDSKGKWWLVGASWRGNMENAFE 616

Query: 123 TSDNVEEFANTIDKEVLEAQKMLQ--------LAAAQRMNTDARKAIFCIIMSGEDYVDA 182
            S N E  A+   K  +  +  L         +A  QRMNTD R+AIF  IMS +DY+DA
Sbjct: 617 VSINNENDASKSKKSKISIEDDLLDDIPDWNIIARQQRMNTDIRRAIFISIMSAQDYLDA 676

Query: 183 FEKLIRLDLSGKQDREIMRVLVDCCLQE---KVFNKYYTVLASKLCEHEKNHKFTLQYCL 242
           F KL +L L  KQ  EI R+++ C L +     +N YY ++A+K+CE   +   + Q+  
Sbjct: 677 FSKLEKLSLKNKQVLEIPRIVLHCLLADSGSNGYNHYYALVANKICERYSHLSKSFQFLF 736

Query: 243 WDQFKEL------------------DTMQLIRSMNLAKFVAEMITSFTLSLAVLKSV 256
           WD  K+                   D  +L+R  N  +F   ++ +  L L V K V
Sbjct: 737 WDVIKKFEDKEFDSESDTDEEDDLDDKEKLLRISNQGRFFGSLLANDILKLDVFKHV 779

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BLAST of Cucsat.G5368.T4 vs. NCBI nr
Match: XP_004149114.1 (nucleolar MIF4G domain-containing protein 1 [Cucumis sativus] >KGN53923.1 hypothetical protein Csa_019148 [Cucumis sativus])

HSP 1 Score: 670 bits (1729), Expect = 2.47e-237
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD
Sbjct: 349 MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 408

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 120
           DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID
Sbjct: 409 DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 468

Query: 121 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 180
           KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL
Sbjct: 469 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 528

Query: 181 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 240
           VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE
Sbjct: 529 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 588

Query: 241 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 300
           MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE
Sbjct: 589 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 648

Query: 301 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 340
           PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM
Sbjct: 649 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 688

[Close]

BLAST of Cucsat.G5368.T4 vs. NCBI nr
Match: XP_008441997.1 (PREDICTED: nucleolar MIF4G domain-containing protein 1 [Cucumis melo] >KAA0041729.1 nucleolar MIF4G domain-containing protein 1 [Cucumis melo var. makuwa] >TYK08400.1 nucleolar MIF4G domain-containing protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 659 bits (1699), Expect = 9.18e-233
Identity = 333/340 (97.94%), Postives = 338/340 (99.41%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIRADDPTAMKSFIVSVQSKVNELKAASG+G QNINGKRMEFMLETICDIKNNKKRSKD
Sbjct: 350 MKIRADDPTAMKSFIVSVQSKVNELKAASGNGSQNINGKRMEFMLETICDIKNNKKRSKD 409

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 120
           DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDL+TTSDNVEEFANTID
Sbjct: 410 DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLTTTSDNVEEFANTID 469

Query: 121 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 180
           KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREI+RVL
Sbjct: 470 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIVRVL 529

Query: 181 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 240
           VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE
Sbjct: 530 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 589

Query: 241 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 300
           MITSFTLSLAVLKSVDLSDIRLLT KRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE
Sbjct: 590 MITSFTLSLAVLKSVDLSDIRLLTPKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 649

Query: 301 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 340
           PLRSGMLFFIKEY+IKTSKVNAEK+KLIKKALNNVEGILM
Sbjct: 650 PLRSGMLFFIKEYVIKTSKVNAEKYKLIKKALNNVEGILM 689

[Close]

BLAST of Cucsat.G5368.T4 vs. NCBI nr
Match: XP_038882894.1 (nucleolar MIF4G domain-containing protein 1 [Benincasa hispida])

HSP 1 Score: 652 bits (1682), Expect = 4.29e-230
Identity = 326/340 (95.88%), Postives = 336/340 (98.82%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIRADDPTAMK+FI+SVQSKVNELKAASGDG QNINGKRMEFMLETICDIKNNKKRSKD
Sbjct: 356 MKIRADDPTAMKNFIISVQSKVNELKAASGDGPQNINGKRMEFMLETICDIKNNKKRSKD 415

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 120
           DPAHHTRIKKWLQKLGVDD+IIRGIKWNKLLDPDKKGQWWLSGD++TTSDNV+EFANTID
Sbjct: 416 DPAHHTRIKKWLQKLGVDDMIIRGIKWNKLLDPDKKGQWWLSGDVTTTSDNVKEFANTID 475

Query: 121 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 180
           KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKL+RLDLSGKQDREIMRVL
Sbjct: 476 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLLRLDLSGKQDREIMRVL 535

Query: 181 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 240
           VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKF+AE
Sbjct: 536 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFIAE 595

Query: 241 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 300
           MI SFTLSLAVLKSVDLSD+RLLT KRIMHFRMLFDAIFER DKLIWNVFTRVAVNPELE
Sbjct: 596 MIASFTLSLAVLKSVDLSDVRLLTPKRIMHFRMLFDAIFERSDKLIWNVFTRVAVNPELE 655

Query: 301 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 340
           PLRSGMLFFIKEYMIKTSKVNAEKFKL+KKALNNVEGILM
Sbjct: 656 PLRSGMLFFIKEYMIKTSKVNAEKFKLVKKALNNVEGILM 695

[Close]

BLAST of Cucsat.G5368.T4 vs. NCBI nr
Match: XP_023517488.1 (nucleolar MIF4G domain-containing protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615 bits (1587), Expect = 7.23e-216
Identity = 304/340 (89.41%), Postives = 324/340 (95.29%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIR DDP AMK+FIVSVQSKVNE+KAASGDG QNINGKRMEFMLETICDIKNNKK+ KD
Sbjct: 343 MKIRGDDPCAMKNFIVSVQSKVNEVKAASGDGQQNINGKRMEFMLETICDIKNNKKKPKD 402

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 120
           DPA+HTRIKKWL KLGV+DIIIRGIKW KLLDPDKKGQWWL+GD++TTS+N+ EFA+TID
Sbjct: 403 DPANHTRIKKWLHKLGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVTTTSENITEFASTID 462

Query: 121 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 180
            EVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKL+RLDLSGKQDREIMRVL
Sbjct: 463 TEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLLRLDLSGKQDREIMRVL 522

Query: 181 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 240
           VDCCLQEKVFNKYYT+LASKLCEHEKNHKFTLQYCLWD FKEL+TMQL+RSMNLAKF AE
Sbjct: 523 VDCCLQEKVFNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLAKFTAE 582

Query: 241 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 300
           MI SFTLSLAVLKSVDLSDI LLT KRIMHFRMLFD+IFERPDKLIWN FTRVA+NPELE
Sbjct: 583 MIASFTLSLAVLKSVDLSDIMLLTPKRIMHFRMLFDSIFERPDKLIWNAFTRVAINPELE 642

Query: 301 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 340
           PLRSGMLFFIKEYMIK +KVN EKFK++KKALNNVEGILM
Sbjct: 643 PLRSGMLFFIKEYMIKGNKVNVEKFKVVKKALNNVEGILM 682

[Close]

BLAST of Cucsat.G5368.T4 vs. NCBI nr
Match: KAG6595483.1 (Nucleolar MIF4G domain-containing protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 613 bits (1581), Expect = 2.21e-215
Identity = 303/340 (89.12%), Postives = 323/340 (95.00%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIR DDP AMK+FIVSVQSKVNE+KAASGDG QNINGKRMEFMLETICDIKNNKK+ KD
Sbjct: 314 MKIRGDDPCAMKNFIVSVQSKVNEVKAASGDGQQNINGKRMEFMLETICDIKNNKKKPKD 373

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 120
           DPA+HTRIKKWL KLGV+DIIIRGIKW KLLDPDKKGQWWL+GD++TTS+N+ EFA+TID
Sbjct: 374 DPANHTRIKKWLHKLGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVTTTSENITEFASTID 433

Query: 121 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 180
            EVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKL+RLDLSGKQDREIMRVL
Sbjct: 434 TEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLLRLDLSGKQDREIMRVL 493

Query: 181 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 240
           VDCCLQEKVFNKYYT+LASKLCEHEKNHKFTLQYCLWD FKEL+TMQL+RSMNLAKF AE
Sbjct: 494 VDCCLQEKVFNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLAKFTAE 553

Query: 241 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 300
           MI  FTLSLAVLKSVDLSDI LLT KRIMHFRMLFD+IFERPDKLIWN FTRVA+NPELE
Sbjct: 554 MIALFTLSLAVLKSVDLSDIMLLTPKRIMHFRMLFDSIFERPDKLIWNAFTRVAINPELE 613

Query: 301 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 340
           PLRSGMLFFIKEYMIK +KVN EKFK++KKALNNVEGILM
Sbjct: 614 PLRSGMLFFIKEYMIKGNKVNVEKFKVVKKALNNVEGILM 653

[Close]

BLAST of Cucsat.G5368.T4 vs. ExPASy TrEMBL
Match: A0A0A0KWL8 (MI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G188970 PE=4 SV=1)

HSP 1 Score: 670 bits (1729), Expect = 1.20e-237
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD
Sbjct: 349 MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 408

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 120
           DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID
Sbjct: 409 DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 468

Query: 121 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 180
           KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL
Sbjct: 469 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 528

Query: 181 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 240
           VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE
Sbjct: 529 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 588

Query: 241 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 300
           MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE
Sbjct: 589 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 648

Query: 301 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 340
           PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM
Sbjct: 649 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 688

[Close]

BLAST of Cucsat.G5368.T4 vs. ExPASy TrEMBL
Match: A0A5D3CAM3 (Nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00080 PE=4 SV=1)

HSP 1 Score: 659 bits (1699), Expect = 4.45e-233
Identity = 333/340 (97.94%), Postives = 338/340 (99.41%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIRADDPTAMKSFIVSVQSKVNELKAASG+G QNINGKRMEFMLETICDIKNNKKRSKD
Sbjct: 350 MKIRADDPTAMKSFIVSVQSKVNELKAASGNGSQNINGKRMEFMLETICDIKNNKKRSKD 409

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 120
           DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDL+TTSDNVEEFANTID
Sbjct: 410 DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLTTTSDNVEEFANTID 469

Query: 121 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 180
           KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREI+RVL
Sbjct: 470 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIVRVL 529

Query: 181 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 240
           VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE
Sbjct: 530 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 589

Query: 241 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 300
           MITSFTLSLAVLKSVDLSDIRLLT KRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE
Sbjct: 590 MITSFTLSLAVLKSVDLSDIRLLTPKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 649

Query: 301 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 340
           PLRSGMLFFIKEY+IKTSKVNAEK+KLIKKALNNVEGILM
Sbjct: 650 PLRSGMLFFIKEYVIKTSKVNAEKYKLIKKALNNVEGILM 689

[Close]

BLAST of Cucsat.G5368.T4 vs. ExPASy TrEMBL
Match: A0A1S3B470 (nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo OX=3656 GN=LOC103485994 PE=4 SV=1)

HSP 1 Score: 659 bits (1699), Expect = 4.45e-233
Identity = 333/340 (97.94%), Postives = 338/340 (99.41%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIRADDPTAMKSFIVSVQSKVNELKAASG+G QNINGKRMEFMLETICDIKNNKKRSKD
Sbjct: 350 MKIRADDPTAMKSFIVSVQSKVNELKAASGNGSQNINGKRMEFMLETICDIKNNKKRSKD 409

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 120
           DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDL+TTSDNVEEFANTID
Sbjct: 410 DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLTTTSDNVEEFANTID 469

Query: 121 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 180
           KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREI+RVL
Sbjct: 470 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIVRVL 529

Query: 181 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 240
           VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE
Sbjct: 530 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 589

Query: 241 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 300
           MITSFTLSLAVLKSVDLSDIRLLT KRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE
Sbjct: 590 MITSFTLSLAVLKSVDLSDIRLLTPKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 649

Query: 301 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 340
           PLRSGMLFFIKEY+IKTSKVNAEK+KLIKKALNNVEGILM
Sbjct: 650 PLRSGMLFFIKEYVIKTSKVNAEKYKLIKKALNNVEGILM 689

[Close]

BLAST of Cucsat.G5368.T4 vs. ExPASy TrEMBL
Match: A0A6J1EB72 (nucleolar MIF4G domain-containing protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111432528 PE=4 SV=1)

HSP 1 Score: 614 bits (1583), Expect = 1.37e-215
Identity = 303/340 (89.12%), Postives = 323/340 (95.00%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIR DDP AMK+FIVSVQSKVNE+KA SGDG QNINGKRMEFMLETICDIKNNKK+ KD
Sbjct: 342 MKIRGDDPCAMKNFIVSVQSKVNEVKAGSGDGQQNINGKRMEFMLETICDIKNNKKKPKD 401

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 120
           DPA+HTRIKKWL KLGV+DIIIRGIKW KLLDPDKKGQWWL+GD++TTS+N+ EFA+TID
Sbjct: 402 DPANHTRIKKWLHKLGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVTTTSENITEFASTID 461

Query: 121 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 180
            EVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKL+RLDLSGKQDREIMRVL
Sbjct: 462 TEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLLRLDLSGKQDREIMRVL 521

Query: 181 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 240
           VDCCLQEKVFNKYYT+LASKLCEHEKNHKFTLQYCLWD FKEL+TMQL+RSMNLAKF AE
Sbjct: 522 VDCCLQEKVFNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLAKFTAE 581

Query: 241 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 300
           MI SFTLSLAVLKSVDLSDI LLT KRIMHFRMLFD+IFERPDKLIWN FTRVA+NPELE
Sbjct: 582 MIASFTLSLAVLKSVDLSDIMLLTPKRIMHFRMLFDSIFERPDKLIWNAFTRVAINPELE 641

Query: 301 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 340
           PLRSGMLFFIKEYMIK +KVN EKFK++KKALNNVEGILM
Sbjct: 642 PLRSGMLFFIKEYMIKGNKVNVEKFKVVKKALNNVEGILM 681

[Close]

BLAST of Cucsat.G5368.T4 vs. ExPASy TrEMBL
Match: A0A6J1D6L2 (nucleolar MIF4G domain-containing protein 1-like OS=Momordica charantia OX=3673 GN=LOC111017795 PE=4 SV=1)

HSP 1 Score: 609 bits (1570), Expect = 1.92e-213
Identity = 303/343 (88.34%), Postives = 325/343 (94.75%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIR DDPT MK+FIVSVQSKVNELKAA GDG QNINGKRMEFMLETICDIKNNKKR KD
Sbjct: 351 MKIRGDDPTGMKNFIVSVQSKVNELKAAPGDGPQNINGKRMEFMLETICDIKNNKKRHKD 410

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLS---TTSDNVEEFAN 120
           DPAHHTRIKKWLQKL V+DI+IRGIKWNKL+DPDKKGQWWLSGD+S   TTS NV EFA+
Sbjct: 411 DPAHHTRIKKWLQKLRVEDILIRGIKWNKLIDPDKKGQWWLSGDMSYMTTTSKNVTEFAS 470

Query: 121 TIDKEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIM 180
           TIDKEV+EAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKL+RLDL G QDREIM
Sbjct: 471 TIDKEVVEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLLRLDLPGMQDREIM 530

Query: 181 RVLVDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKF 240
           RV+V+CCLQEKVFNKYYT+LASKLC H+KNHKFTLQYCLWD FKEL+TMQLIRSMNLAKF
Sbjct: 531 RVVVECCLQEKVFNKYYTILASKLCAHDKNHKFTLQYCLWDHFKELETMQLIRSMNLAKF 590

Query: 241 VAEMITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNP 300
           V EMITSFTLSLAVLKSVDLSD+RLLT KRIMHFRMLFD IF+RPDKLIWNVFTRVAVNP
Sbjct: 591 VVEMITSFTLSLAVLKSVDLSDVRLLTPKRIMHFRMLFDEIFKRPDKLIWNVFTRVAVNP 650

Query: 301 ELEPLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 340
           ELEPLRSGMLFFI+EYMI+T+KV+A+KFKL+KKALNNVEG+LM
Sbjct: 651 ELEPLRSGMLFFIREYMIRTNKVDAKKFKLVKKALNNVEGVLM 693

[Close]

BLAST of Cucsat.G5368.T4 vs. TAIR 10
Match: AT5G17930.1 (MIF4G domain-containing protein / MA3 domain-containing protein )

HSP 1 Score: 477.6 bits (1228), Expect = 8.1e-135
Identity = 233/340 (68.53%), Postives = 286/340 (84.12%), Query Frame = 0


Query: 1   MKIRADDPTAMKSFIVSVQSKVNELKAASGDGLQNINGKRMEFMLETICDIKNNKKRSKD 60
           MKIR+DDP AMK+FI+S+Q+K NELK +S DG   +N   ME MLETI  IKNNK R+K+
Sbjct: 446 MKIRSDDPLAMKTFIISIQNKANELKTSS-DGQTQMNNMMMEKMLETISAIKNNKLRAKE 505

Query: 61  DPAHHTRIKKWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLSTTSDNVEEFANTID 120
           D   +TR+KKWLQKL V+++++RG+ W+KLLDP+KKGQWWLSGDL   S+  E+ A T+D
Sbjct: 506 DSVQNTRVKKWLQKLRVEEVLLRGLTWSKLLDPEKKGQWWLSGDLVVDSNTAEDIAETMD 565

Query: 121 KEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIMRVL 180
            EV+EAQKML+LA AQRMNTD+RKAIFC+IMS EDY+DAFEKL+RLDL GKQDREIMRVL
Sbjct: 566 AEVVEAQKMLKLAEAQRMNTDSRKAIFCVIMSSEDYIDAFEKLLRLDLPGKQDREIMRVL 625

Query: 181 VDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAE 240
           V+CCLQEK FNK+YTVLASKLCEH+KNHKFTLQYC+WD FKEL++M L RSM+LAKFVAE
Sbjct: 626 VECCLQEKAFNKFYTVLASKLCEHDKNHKFTLQYCIWDHFKELESMSLQRSMHLAKFVAE 685

Query: 241 MITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELE 300
           +I +F LSLAVLKSVDL++   LT KRIMHFRMLF+AIFE P+ L+WN+FTR+A+NPE E
Sbjct: 686 IIVTFNLSLAVLKSVDLANPVELTPKRIMHFRMLFEAIFEHPENLVWNLFTRIALNPEYE 745

Query: 301 PLRSGMLFFIKEYMIKTSKVNAEKFKLIKKALNNVEGILM 341
            LR G+ FF+KEY++K +K    KF+  K+ALNN EG+LM
Sbjct: 746 ALRDGIKFFVKEYVVKNNKAIYGKFRKAKEALNNAEGLLM 784

[Close]

BLAST of Cucsat.G5368.T4 vs. TAIR 10
Match: AT1G80930.1 (MIF4G domain-containing protein / MA3 domain-containing protein )

HSP 1 Score: 69.7 bits (169), Expect = 5.1e-12
Identity = 48/178 (26.97%), Postives = 92/178 (51.69%), Query Frame = 0


Query: 103 GDLSTTSDNVEEFANTIDKEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEK 162
           G  +++ DN EE  +  D+E  E  ++        +N   R+ I+  IMS  D+ +A  K
Sbjct: 614 GSDASSEDNDEE-EDESDEEDEEQMRIRDETETNLVN--LRRTIYLTIMSSVDFEEAGHK 673

Query: 163 LIRLDLSGKQDREIMRVLVDCCLQEKVFNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKE 222
           L+++ L   Q+ E+  +L++CC QE+ + +YY +L  + C   K H+   + C   Q+  
Sbjct: 674 LLKIKLEPGQEMELCIMLLECCSQERTYLRYYGLLGQRFCMINKIHQENFEKCFVQQYSM 733

Query: 223 LDTMQLIRSMNLAKFVAEMITSFTLSLAVLKSVDLSDIRLLTAKRIMHFRMLFDAIFE 281
           +  ++  +  N+AKF A ++ +  L   VL  + L++    ++ RI   ++LF  + E
Sbjct: 734 IHRLETNKLRNVAKFFAHLLGTDALPWHVLAYIRLTEEDTTSSSRIF-IKILFQELSE 787

The following BLAST results are available for this feature:

BLAST of Cucsat.G5368.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5C9Z4	4.6e-50	35.56	Nucleolar MIF4G domain-containing protein 1 OS=Homo sapiens OX=9606 GN=NOM1 PE=1...	[more]
Q3UFM5	1.9e-48	34.60	Nucleolar MIF4G domain-containing protein 1 OS=Mus musculus OX=10090 GN=Nom1 PE=...	[more]
Q9W020	5.8e-37	28.93	Nucleolar MIF4G domain-containing protein 1 homolog OS=Drosophila melanogaster O...	[more]
O13971	3.2e-27	28.80	Suppressor of glycerol defect protein 1 OS=Schizosaccharomyces pombe (strain 972...	[more]
Q06132	2.2e-23	29.63	Suppressor of glycerol defect protein 1 OS=Saccharomyces cerevisiae (strain ATCC...	[more]

BLAST of Cucsat.G5368.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149114.1	2.47e-237	100.00	nucleolar MIF4G domain-containing protein 1 [Cucumis sativus] >KGN53923.1 hypoth...	[more]
XP_008441997.1	9.18e-233	97.94	PREDICTED: nucleolar MIF4G domain-containing protein 1 [Cucumis melo] >KAA004172...	[more]
XP_038882894.1	4.29e-230	95.88	nucleolar MIF4G domain-containing protein 1 [Benincasa hispida]	[more]
XP_023517488.1	7.23e-216	89.41	nucleolar MIF4G domain-containing protein 1 [Cucurbita pepo subsp. pepo]	[more]
KAG6595483.1	2.21e-215	89.12	Nucleolar MIF4G domain-containing protein 1, partial [Cucurbita argyrosperma sub...	[more]

BLAST of Cucsat.G5368.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWL8	1.20e-237	100.00	MI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G188970 PE=4 SV=...	[more]
A0A5D3CAM3	4.45e-233	97.94	Nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B470	4.45e-233	97.94	nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]
A0A6J1EB72	1.37e-215	89.12	nucleolar MIF4G domain-containing protein 1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1D6L2	1.92e-213	88.34	nucleolar MIF4G domain-containing protein 1-like OS=Momordica charantia OX=3673 ...	[more]

BLAST of Cucsat.G5368.T4 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17930.1	8.1e-135	68.53	MIF4G domain-containing protein / MA3 domain-containing protein	[more]
AT1G80930.1	5.1e-12	26.97	MIF4G domain-containing protein / MA3 domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003891	Initiation factor eIF-4 gamma, MA3	SMART	SM00544	ma3_7	coord: 142..248 e-value: 8.9E-18 score: 75.1
IPR003891	Initiation factor eIF-4 gamma, MA3	PFAM	PF02847	MA3	coord: 143..244 e-value: 4.8E-22 score: 78.1
IPR003891	Initiation factor eIF-4 gamma, MA3	PROSITE	PS51366	MI	coord: 141..257 score: 21.850531
IPR016021	MIF4G-like domain superfamily	GENE3D	1.25.40.180		coord: 1..124 e-value: 4.0E-10 score: 41.1
None	No IPR available	PANTHER	PTHR18034	CELL CYCLE CONTROL PROTEIN CWF22-RELATED	coord: 1..337
None	No IPR available	PANTHER	PTHR18034:SF4	NUCLEOLAR MIF4G DOMAIN-CONTAINING PROTEIN 1	coord: 1..337

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G5368	Cucsat.G5368	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G5368.T4.E1	Cucsat.G5368.T4.E1	exon
Cucsat.G5368.T4.E2	Cucsat.G5368.T4.E2	exon
Cucsat.G5368.T4.E3	Cucsat.G5368.T4.E3	exon
Cucsat.G5368.T4.E4	Cucsat.G5368.T4.E4	exon
Cucsat.G5368.T4.E5	Cucsat.G5368.T4.E5	exon
Cucsat.G5368.T4.E6	Cucsat.G5368.T4.E6	exon
Cucsat.G5368.T4.E7	Cucsat.G5368.T4.E7	exon
Cucsat.G5368.T4.E8	Cucsat.G5368.T4.E8	exon
Cucsat.G5368.T4.E9	Cucsat.G5368.T4.E9	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G5368.T4.C5	Cucsat.G5368.T4.C5	CDS
Cucsat.G5368.T4.C4	Cucsat.G5368.T4.C4	CDS
Cucsat.G5368.T4.C3	Cucsat.G5368.T4.C3	CDS
Cucsat.G5368.T4.C2	Cucsat.G5368.T4.C2	CDS
Cucsat.G5368.T4.C1	Cucsat.G5368.T4.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G5368.T4	Cucsat.G5368.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006417	regulation of translation
biological_process	GO:0042274	ribosomal small subunit biogenesis
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003723	RNA binding