Cucsat.G4812.T13 (mRNA) Cucumber (B10) v3

BLAST of Cucsat.G4812.T13 vs. ExPASy Swiss-Prot
Match: Q9LF10 (Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g16180 PE=3 SV=2)

HSP 1 Score: 449.9 bits (1156), Expect = 4.8e-125
Identity = 274/608 (45.07%), Postives = 364/608 (59.87%), Query Frame = 0

Query: 33  NPISATSTPSQSSVLPEPPSISNAAVNLRTAPWMKAPLHLQPQQQEEEGVDPANPKRRNG 92
           N  S  +  SQ     E   +S+AA+ + TAPWMK PL L+P        +  + K+RN 
Sbjct: 56  NSSSRRAKSSQFDQFRENRGVSDAAIKVPTAPWMKGPLLLRPD-------EILDTKKRN- 115

Query: 93  SDGSGRDKCSRALGDSGIDKTGKYAMRRIAKSIGKLRRNGDLGETRM-KLEEVEF-GDFD 152
                 +K  +AL        GK AM++I +++ KL  + D  ET+M  L E E+ G  +
Sbjct: 116 KPRKVEEKTFKALNRRESGVRGKKAMKKIVRNVEKLDEDSDSEETQMDDLSEFEYLGRIE 175

Query: 153 LEGFEESGTRRRMPWEKDDDGIVLRRMKKKTV-TSAELNLDRVLLERLKGEASKMEKWVK 212
            +   +     +MPWE++++  +LRRMKK++V T+AEL LD  LL RL+ EASKM KWV 
Sbjct: 176 EKVESKDRFGGKMPWEREEERFILRRMKKESVPTTAELILDEGLLNRLRREASKMRKWVN 235

Query: 213 VNKVGVTQDVVNQIQFMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALV 272
           V K GVT+ VVN+I+ MW+ NELAM++FDVPL RNM+RA+EI+EMKTGG+VV SKK  LV
Sbjct: 236 VRKAGVTELVVNKIKSMWKLNELAMVRFDVPLCRNMERAQEIIEMKTGGLVVLSKKEFLV 295

Query: 273 IYRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEAS 332
           +YRG                                    Y S                 
Sbjct: 296 VYRG---------------------------------GPSYSS----------------- 355

Query: 333 SFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPF 392
                  E    +S SLYERE DRLLD LGPR++DWWM +P PVDAD+L EVV GYM P 
Sbjct: 356 -------EGQDEISSSLYEREADRLLDGLGPRYMDWWMRRPFPVDADLLPEVVNGYMTPS 415

Query: 393 RRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALK 452
           RRCPP T+  LTD  L +LR +A  LP HFVLGRN  LQGLA++I+KLWEK +IAKIA+K
Sbjct: 416 RRCPPNTRAKLTDEELTYLRNIAQPLPFHFVLGRNYGLQGLASAIVKLWEKCIIAKIAIK 475

Query: 453 WGVPNTDNEQMALELKNLTGGTLLLRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQL 512
           WG  NT+NE+MA EL+ LTGG L+LRNK++I+LYRG DFL   VAD +  RE  L R+Q 
Sbjct: 476 WGALNTNNEEMADELRYLTGGVLILRNKYLIVLYRGKDFLSDEVADLVEDRERLLSRYQH 535

Query: 513 HEENSRLKASEFFCFDT--ENMEERGKAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKG 572
            EE  R    E     T  + ++E  K+GTL +F+++   + ++        L+ EAEK 
Sbjct: 536 FEETKRESDIELLEVVTNGKQLKETNKSGTLLEFQELQRKFGEMD----PRNLETEAEKA 594

Query: 573 KIIRGLRMQERRLKILNFKVEKSTKELTKLNASWRRVEPDADQELITNEERICFRKMGLK 632
           ++ + L+ QE +L IL  K+EKS  EL KLN+ W+  E D D E++TNEER C R++GLK
Sbjct: 596 RLEKELKSQEHKLSILKSKIEKSNMELFKLNSLWKPSEGDDDIEILTNEERECLRRIGLK 594

Query: 633 MDSCLTLG 636
           M+S L LG
Sbjct: 656 MNSSLVLG 594

BLAST of Cucsat.G4812.T13 vs. ExPASy Swiss-Prot
Match: Q6YYA3 (Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0360100 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.4e-108
Identity = 224/486 (46.09%), Postives = 310/486 (63.79%), Query Frame = 0

Query: 155 EESGTRRRMPW---EKDDDGIVLRRMKKKTV-TSAELNLDRVLLERLKGEASKMEKWVKV 214
           E+ G RR +PW     ++  +VLRR KK  V T AE  L+   LERL+  A   E+W + 
Sbjct: 117 EKKGIRRAVPWAAARDEETKVVLRREKKTRVPTRAETELEAGELERLRRAARGKERWARA 176

Query: 215 NKVGVTQDVVNQIQFMWER-NELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALV 274
            K G+T +VV +++  W +  ELA ++   PL R MDRAREI+E+KTGG+VVW++     
Sbjct: 177 KKAGITDEVVEEVRGQWAKGQELAGVRIVEPLRRCMDRAREILEIKTGGLVVWTRGGIHF 236

Query: 275 IYRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEAS 334
           +YRG +Y   L+++ + +  ++    +   T  + +  G Y S      NDND    EA 
Sbjct: 237 VYRGSSY---LENAKRHRDFVNYNEELSPVTSNNPTSQGKYWSKDETLTNDND----EAD 296

Query: 335 SFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPF 394
                  ++ +P+ G+LYERE +RLLD LGPRFIDWW + PLPVDAD+L EVVP +  PF
Sbjct: 297 D------KDDKPIKGTLYEREVNRLLDSLGPRFIDWWWNTPLPVDADLLPEVVPDFKTPF 356

Query: 395 RRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALK 454
           R+CPP  +  L D  L +LRK A  LPTHFVLGRN KLQGLAA+ILKLWEKS+IAK+A+K
Sbjct: 357 RQCPPGVRPALADEELTYLRKHARPLPTHFVLGRNTKLQGLAAAILKLWEKSLIAKVAVK 416

Query: 455 WGVPNTDNEQMALELKNLTGGTLLLRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQL 514
            G+ NT++EQMA  LK LTGGT++LRNK  II+YRG DFLP GVA+S+I+RE ++   Q 
Sbjct: 417 VGIQNTNHEQMARNLKRLTGGTVILRNKDYIIIYRGKDFLPGGVAESVIERESQVHDQQA 476

Query: 515 HEENSRLKASEFFCFDTENMEERGKAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKI 574
            EE +RLK ++          ER   GT  +++D    +   +T     R+Q EA+K ++
Sbjct: 477 KEEEARLKMADSLQMIVGLSSERSYVGTFREYQDFHDSHARRTT-ENNFRIQLEAKKHRL 536

Query: 575 IRGLRMQERRLKILNFKVEKSTKELTKLNASWRRVEPDADQELITNEERICFRKMGLKMD 634
            + L+ QE RL +L  K+E+S + L KL++SW   + D D+EL+T EER  FRK+GLKMD
Sbjct: 537 EKELKDQEWRLSMLTKKIERSNQVLAKLHSSWSPSKKDGDRELLTEEERRIFRKIGLKMD 588

Query: 635 SCLTLG 636
             + LG
Sbjct: 597 EHVLLG 588

BLAST of Cucsat.G4812.T13 vs. ExPASy Swiss-Prot
Match: Q9FYT6 (Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Zea mays OX=4577 GN=CRS1 PE=1 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 8.9e-103
Identity = 215/485 (44.33%), Postives = 295/485 (60.82%), Query Frame = 0

Query: 156 ESGTRRRMPW-EKDDDG--IVLRRMKK-KTVTSAELNLDRVLLERLKGEASKMEKWVKVN 215
           E    R +PW    D+G  + LRR KK +  T AE  L+   L RL+  A  + +W +  
Sbjct: 133 EKEKSRAVPWAAARDEGLKVALRREKKPREPTRAETELETHELRRLRRLARGIGRWARAK 192

Query: 216 KVGVTQDVVNQIQFMW-ERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALVI 275
           K GVT +VV +++  W    ELA ++   PL R+MDRAREI+E+KTGG+VVW+K +   +
Sbjct: 193 KAGVTDEVVKEVRREWASGEELAAVRIVEPLRRSMDRAREILEIKTGGLVVWTKGDMHFV 252

Query: 276 YRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASS 335
           YRG  Y  N KHS                    F  + H +     +     G+ +E   
Sbjct: 253 YRGSKYQQNAKHSHT------------------FLTNVHKDDAFQENDQSICGQKDE--- 312

Query: 336 FFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFR 395
                    +P+ G+LYERE +RLLD LGPRF+DWW   PLPVDAD+L E VPG   P+R
Sbjct: 313 ---------EPVKGTLYEREVNRLLDTLGPRFVDWWWDTPLPVDADLLPEFVPGSKTPYR 372

Query: 396 RCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKW 455
            CPP  +  L D  L +LRKLA  LPTHF LGRN +LQGLAA+ILKLWEKS+IAKIA+K 
Sbjct: 373 LCPPGVRPTLADEELTYLRKLARLLPTHFALGRNTRLQGLAAAILKLWEKSLIAKIAVKI 432

Query: 456 GVPNTDNEQMALELKNLTGGTLLLRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQLH 515
           G+ NT+NEQMA  LK+LTGGT++LRNK  IILYRG DFLP GVA ++IQRE ++   Q+ 
Sbjct: 433 GIQNTNNEQMAWNLKHLTGGTVILRNKDFIILYRGKDFLPGGVAQTVIQREAQVHDEQVK 492

Query: 516 EENSRLKASEFFCFDTENMEERGKAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKII 575
           EE +RLKA +      E + E    GT  +++     +   +T ++ + ++ EAEK ++ 
Sbjct: 493 EEEARLKAVDSLQMVGE-LSEESSLGTFREYQGFHAKFVHENTENSNTMIELEAEKYRLE 552

Query: 576 RGLRMQERRLKILNFKVEKSTKELTKLNASWRRVEPDADQELITNEERICFRKMGLKMDS 635
           + L+  E +L +LN K+E+S + L KL++SW   E  AD+E +T EE+I FR++G KMD 
Sbjct: 553 KELKDHEWKLSVLNKKIERSNQALAKLHSSWSPSEQSADREHLTEEEKIMFRRIGRKMDG 586

BLAST of Cucsat.G4812.T13 vs. ExPASy Swiss-Prot
Match: Q2R1U8 (CRM-domain containing factor CFM3, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=CFM3 PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 4.5e-70
Identity = 179/498 (35.94%), Postives = 267/498 (53.61%), Query Frame = 0

Query: 147 GDFDLEGFEESGTRRRMPWE----KDDDGIVLRRMKKKTVTSAELNLDRVLLERLKGEAS 206
           G FD +  E +    R PWE      + G    R  +     AEL L    L RL+    
Sbjct: 134 GGFD-DDAESALVDARFPWELPMPPPEAG---PRAARSKAWMAELTLPEAELRRLRHAGM 193

Query: 207 KMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVW 266
           +++  +KV   GVT+++V +I+  W  +E+  +K     + NM    EI+E KTGG+V+W
Sbjct: 194 RLKSRIKVGGAGVTREIVERIRDRWRNDEVVRIKVTGTPALNMRLFHEILERKTGGLVIW 253

Query: 267 SKKNALVIYRGCNY--PLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSIND 326
               ++ +YRG  Y  P   K ++K    +  ++ +K E   H  L     +     + D
Sbjct: 254 RSGTSVSLYRGVAYDIPEPTKGTSKNTQTLGMKSSIK-EPPGHSLLPNEKVN----EMQD 313

Query: 327 NDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQE 386
           N+G     +     +   ++P+    YE E D+LLD+LGPR+ DW    P PVDAD+L  
Sbjct: 314 NNGALVSNAE----KDTLVEPVPEIKYEDEIDKLLDELGPRYDDWPRPDPSPVDADLLPA 373

Query: 387 VVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEK 446
            VPGY PPFR  P   + +L+     +LR+LA  LP HF LGR+R+LQGLAA+++KLWEK
Sbjct: 374 TVPGYKPPFRVLPYGVRPSLSRRDTTNLRRLARGLPPHFALGRSRQLQGLAAAMVKLWEK 433

Query: 447 SMIAKIALKWGVPNTDNEQMALELKNLTGGTLLLRNKFVIILYRGNDFLPVGVADSIIQR 506
           S IAKIALK GV  T +E+MA ++K LTGG +L RN   ++ YRG DFL   +A+ +++R
Sbjct: 434 SSIAKIALKRGVQLTTSERMAEDIKKLTGGVMLSRNNDFMVFYRGKDFLSPELAEKLLER 493

Query: 507 EVELQRWQ---LHEENSRLKASEFFCFDTENMEERGKAGTLSDFKDITVGYEDLSTGSTE 566
           E    RW      EE +RL A+  F   TE   E   AGTL +  +    Y +    + E
Sbjct: 494 E----RWAKSLQDEEQARLNAASSFSSRTEAPVEPTVAGTLGETLEANSKYGNKLDENYE 553

Query: 567 SRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKELTKLNASWRRVEPDADQELITNEE 626
           +++    E  +    +R  E +L++   K+EK+ + L K+  + +  E     E IT+EE
Sbjct: 554 NKMTRTVEAARHADLVRKLEWKLQLAQKKIEKAERVLGKVETALKPTEGIQPPETITDEE 613

Query: 627 RICFRKMGLKMDSCLTLG 636
           R  FRK+GL+M + L LG
Sbjct: 614 RFMFRKLGLRMKAFLLLG 614

BLAST of Cucsat.G4812.T13 vs. ExPASy Swiss-Prot
Match: F4J2U9 (CRM-domain containing factor CFM3A, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CFM3A PE=2 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.9e-68
Identity = 167/460 (36.30%), Postives = 247/460 (53.70%), Query Frame = 0

Query: 178 KKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKF 237
           K+   + AE+ L    L RL+    +    +++   GVTQ  V+ I+  W+  E+  LK 
Sbjct: 210 KENRYSLAEMTLPESELRRLRNLTFRTASKMRIRGGGVTQVAVDAIKEKWKSAEIVRLKI 269

Query: 238 DVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISPQNPVK 297
           +   + NM +  EI+E KTGG+V+W    ++ +YRG +Y L      K++         +
Sbjct: 270 EGASALNMRKMHEILEKKTGGLVIWRSGTSISLYRGVSYELPSGKWNKQR---------R 329

Query: 298 VETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDD 357
            ET     +  H E+      +D      +         +  Q      YE E D LLDD
Sbjct: 330 EETPPEAVIENHDETTTMVDKSDEKVHLPQLEQETTSVEKKDQTSPVVEYEDELDELLDD 389

Query: 358 LGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPT 417
           LGPRF+DW    PLPVDAD+L   +P Y PPFR  P   + +L       LR+LA S+P 
Sbjct: 390 LGPRFMDWPGDNPLPVDADLLPGAIPDYEPPFRVLPYGVRSSLGPKEATALRRLARSIPP 449

Query: 418 HFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLLRNK 477
           HF LGR+R+LQGLA ++++LWEKSM+AKIA+K GV +T +E+MA +LK LTGG +L RNK
Sbjct: 450 HFALGRSRQLQGLATAMVRLWEKSMLAKIAIKRGVQSTTSERMAEDLKKLTGGIMLSRNK 509

Query: 478 FVIILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCF-DTENMEERGKAG 537
             ++ YRG +FL   VAD+++++E  ++  Q  EE +RL+ S       TE   +   AG
Sbjct: 510 DFLVFYRGKNFLSREVADALVEQERFVRTLQDEEEQARLRGSSALIVPSTEPANKLVSAG 569

Query: 538 TLSDFKDITVGY-EDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKELT 597
           TL +  D T  + ++L        ++ E E  +    +R  ER+L     K+ K+ + L 
Sbjct: 570 TLGETLDATGKWGKNLDDDDHSDEVKQEVEILRHENLVRKLERKLAFAERKLLKAERGLA 629

Query: 598 KLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 636
           K+    +  E   D E IT+EER  FRK+GLKM + L LG
Sbjct: 630 KVEVCLKPAEQREDPESITDEERFMFRKLGLKMKAFLLLG 660

BLAST of Cucsat.G4812.T13 vs. NCBI nr
Match: XP_011649926.1 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic [Cucumis sativus] >KGN63131.1 hypothetical protein Csa_021894 [Cucumis sativus])

HSP 1 Score: 1256 bits (3251), Expect = 0.0
Identity = 632/635 (99.53%), Postives = 633/635 (99.69%), Query Frame = 0

Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL
Sbjct: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRMKLEEVEFG FDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGGFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP
Sbjct: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISPQNPVKVET 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLKHSTKKQVHISPQNPVKVET
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKHSTKKQVHISPQNPVKVET 300

Query: 301 DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP 360
           DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP
Sbjct: 301 DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP 360

Query: 361 RFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV 420
           RFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV
Sbjct: 361 RFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV 420

Query: 421 LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLLRNKFVI 480
           LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLLRNKFVI
Sbjct: 421 LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLLRNKFVI 480

Query: 481 ILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERGKAGTLSD 540
           ILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERGKAGTLSD
Sbjct: 481 ILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERGKAGTLSD 540

Query: 541 FKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKELTKLNAS 600
           FKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKELTKLNAS
Sbjct: 541 FKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKELTKLNAS 600

Query: 601 WRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635
           WRRVEPDADQELITNEERICFRKMGLKMDSCLTLG
Sbjct: 601 WRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635

BLAST of Cucsat.G4812.T13 vs. NCBI nr
Match: XP_008441260.1 (PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic [Cucumis melo])

HSP 1 Score: 1134 bits (2932), Expect = 0.0
Identity = 579/642 (90.19%), Postives = 599/642 (93.30%), Query Frame = 0

Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSS LP  LIPPSFRSHS LLHLSTH+PISAT TPSQSSVL EPPSISNAA+NL
Sbjct: 1   MLPTTSFSSPLPTILIPPSFRSHSSLLHLSTHHPISATFTPSQSSVLSEPPSISNAALNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQ QQ  EEGVDPANPKRRNGSD +G DKCSRALGD GI+KTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQTQQ--EEGVDPANPKRRNGSDRNGGDKCSRALGDRGIEKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRM LEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIV RRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMNLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVFRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           T+TSAELNLDR+LLERLK +ASKMEKWVKVNKVGVTQDVVN IQ  WERNELAMLKFDVP
Sbjct: 181 TMTSAELNLDRLLLERLKAKASKMEKWVKVNKVGVTQDVVNNIQLKWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHIS-------PQ 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLK STK QV  S        Q
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKPSTKMQVDSSCSKTQVLQQ 300

Query: 301 NPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDR 360
           NPVKVETDTH SLSGHYESGL++S ND+DGEWEEASSFFL+RH NLQPLSGSLYERETDR
Sbjct: 301 NPVKVETDTHSSLSGHYESGLDQSRNDDDGEWEEASSFFLMRHGNLQPLSGSLYERETDR 360

Query: 361 LLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420
           LLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH
Sbjct: 361 LLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420

Query: 421 SLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLL 480
           SLPTHFVLGRNR LQGLA +ILKLWEKSMIAKIALKWGVPNT+NEQMA ELK LTGGTLL
Sbjct: 421 SLPTHFVLGRNRGLQGLAGAILKLWEKSMIAKIALKWGVPNTNNEQMAFELKKLTGGTLL 480

Query: 481 LRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERG 540
           LRNKF+IILYRGNDFLPVGVADSIIQRE+ELQRWQLHEENSRLKASEFFCFDT NMEERG
Sbjct: 481 LRNKFLIILYRGNDFLPVGVADSIIQRELELQRWQLHEENSRLKASEFFCFDTGNMEERG 540

Query: 541 KAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKE 600
           KAGTLSDFKD+TV YEDLST STES+LQAEA++GKIIR LRMQERRLKILNFKVEKSTKE
Sbjct: 541 KAGTLSDFKDVTVEYEDLSTESTESKLQAEAKRGKIIRELRMQERRLKILNFKVEKSTKE 600

Query: 601 LTKLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635
           LTKLNASW RVEPDADQELITNEERICFRKMGLKMDSCLTLG
Sbjct: 601 LTKLNASWSRVEPDADQELITNEERICFRKMGLKMDSCLTLG 640

BLAST of Cucsat.G4812.T13 vs. NCBI nr
Match: TYK06679.1 (chloroplastic group IIA intron splicing facilitator CRS1 [Cucumis melo var. makuwa])

HSP 1 Score: 1134 bits (2932), Expect = 0.0
Identity = 579/642 (90.19%), Postives = 599/642 (93.30%), Query Frame = 0

Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSS LP  LIPPSFRSHS LLHLSTH+PISAT TPSQSSVL EPPSISNAA+NL
Sbjct: 1   MLPTTSFSSPLPTILIPPSFRSHSSLLHLSTHHPISATFTPSQSSVLSEPPSISNAALNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQ QQ  EEGVDPANPKRRNGSD +G DKCSRALGD GI+KTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQTQQ--EEGVDPANPKRRNGSDRNGGDKCSRALGDRGIEKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRM LEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIV RRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMNLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVFRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           T+TSAELNLDR+LLERLK +ASKMEKWVKVNKVGVTQDVVN IQ  WERNELAMLKFDVP
Sbjct: 181 TMTSAELNLDRLLLERLKAKASKMEKWVKVNKVGVTQDVVNNIQLKWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHIS-------PQ 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLK STK QV  S        Q
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKPSTKMQVDSSCSKTQVLQQ 300

Query: 301 NPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDR 360
           NPVKVETDTH SLSGHYESGL++S ND+DGEWEEASSFFL+RH NLQPLSGSLYERETDR
Sbjct: 301 NPVKVETDTHSSLSGHYESGLDQSRNDDDGEWEEASSFFLMRHGNLQPLSGSLYERETDR 360

Query: 361 LLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420
           LLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH
Sbjct: 361 LLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420

Query: 421 SLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLL 480
           SLPTHFVLGRNR LQGLA +ILKLWEKSMIAKIALKWGVPNT+NEQMA ELK LTGGTLL
Sbjct: 421 SLPTHFVLGRNRGLQGLAGAILKLWEKSMIAKIALKWGVPNTNNEQMAFELKKLTGGTLL 480

Query: 481 LRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERG 540
           LRNKF+IILYRGNDFLPVGVADSIIQRE+ELQRWQLHEENSRLKASEFFCFDT NMEERG
Sbjct: 481 LRNKFLIILYRGNDFLPVGVADSIIQRELELQRWQLHEENSRLKASEFFCFDTGNMEERG 540

Query: 541 KAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKE 600
           KAGTLSDFKD+TV YEDLST STES+LQAEA++GKIIR LRMQERRLKILNFKVEKSTKE
Sbjct: 541 KAGTLSDFKDVTVEYEDLSTESTESKLQAEAKRGKIIRELRMQERRLKILNFKVEKSTKE 600

Query: 601 LTKLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635
           LTKLNASW RVEPDADQELITNEERICFRKMGLKMDSCLTLG
Sbjct: 601 LTKLNASWSRVEPDADQELITNEERICFRKMGLKMDSCLTLG 640

BLAST of Cucsat.G4812.T13 vs. NCBI nr
Match: XP_038884367.1 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1048 bits (2710), Expect = 0.0
Identity = 542/643 (84.29%), Postives = 575/643 (89.42%), Query Frame = 0

Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFS   PR LIPPSFRS S LLH S H PISATSTPSQSSVL EPPS SNAAVNL
Sbjct: 1   MLPTTSFS---PRILIPPSFRSDSTLLHFSIHKPISATSTPSQSSVLSEPPSTSNAAVNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMK PL+LQPQQ  EEGVD A P+RRNGSDG+G +KCSRAL D  IDKTGKYAMRR
Sbjct: 61  RTAPWMKGPLYLQPQQ--EEGVDLAKPERRNGSDGNGGEKCSRALADGRIDKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKL++NGD GETRMKLEEVEFG F LEGFEESGTRRRMPWEK D+GIVLRR KKK
Sbjct: 121 IAKSIGKLQKNGDSGETRMKLEEVEFGGFYLEGFEESGTRRRMPWEK-DNGIVLRRTKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           TVTSAELNLD++LLERL+  ASKMEKWVKVNKVGVT+DVVN+IQF+WERNELAMLKFDVP
Sbjct: 181 TVTSAELNLDKMLLERLRSAASKMEKWVKVNKVGVTEDVVNKIQFIWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQV--------HISP 300
           LSRNMDRAREIVEMKTGGMVVWSKKN LV+YRGCNYPL LKHST+ QV         +  
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNGLVVYRGCNYPLGLKHSTEMQVDSSYCSKTQVMQ 300

Query: 301 QNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETD 360
           QNPVK+ETD+ FS   HYE+ LN+SINDNDGEWEE S+ FL+   N QPLSGSLYERETD
Sbjct: 301 QNPVKLETDSDFS-GKHYENDLNQSINDNDGEWEETSTSFLMHQGNFQPLSGSLYERETD 360

Query: 361 RLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLA 420
           RLLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPP+TK NLTDA L HLRKLA
Sbjct: 361 RLLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPFTKPNLTDAELLHLRKLA 420

Query: 421 HSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTL 480
           HSLPTHFVLGRNRKLQGLAA+ILKLWEKS+IAKIALKWGVPNT+NEQMA ELK+LTGGTL
Sbjct: 421 HSLPTHFVLGRNRKLQGLAAAILKLWEKSLIAKIALKWGVPNTNNEQMACELKSLTGGTL 480

Query: 481 LLRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEER 540
           LLRNKF+IILYRG DFLPVGVA+SIIQRE+ELQRWQLHEENSRLKASE FCFDT NMEER
Sbjct: 481 LLRNKFLIILYRGKDFLPVGVANSIIQREMELQRWQLHEENSRLKASELFCFDTGNMEER 540

Query: 541 GKAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTK 600
            KAGTLSDFKDITV Y+DLSTGSTES+LQA+AEKGKIIR LRMQERRL  LNFKVEKS K
Sbjct: 541 AKAGTLSDFKDITVEYQDLSTGSTESKLQADAEKGKIIRELRMQERRLSNLNFKVEKSIK 600

Query: 601 ELTKLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635
           ELTKLNASWR VEPDADQE ITNEERICFRKMGLKMDSCLTLG
Sbjct: 601 ELTKLNASWRHVEPDADQEFITNEERICFRKMGLKMDSCLTLG 636

BLAST of Cucsat.G4812.T13 vs. NCBI nr
Match: XP_038884368.1 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform X3 [Benincasa hispida])

HSP 1 Score: 1048 bits (2710), Expect = 0.0
Identity = 542/643 (84.29%), Postives = 575/643 (89.42%), Query Frame = 0

Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFS   PR LIPPSFRS S LLH S H PISATSTPSQSSVL EPPS SNAAVNL
Sbjct: 1   MLPTTSFS---PRILIPPSFRSDSTLLHFSIHKPISATSTPSQSSVLSEPPSTSNAAVNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMK PL+LQPQQ  EEGVD A P+RRNGSDG+G +KCSRAL D  IDKTGKYAMRR
Sbjct: 61  RTAPWMKGPLYLQPQQ--EEGVDLAKPERRNGSDGNGGEKCSRALADGRIDKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKL++NGD GETRMKLEEVEFG F LEGFEESGTRRRMPWEK D+GIVLRR KKK
Sbjct: 121 IAKSIGKLQKNGDSGETRMKLEEVEFGGFYLEGFEESGTRRRMPWEK-DNGIVLRRTKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           TVTSAELNLD++LLERL+  ASKMEKWVKVNKVGVT+DVVN+IQF+WERNELAMLKFDVP
Sbjct: 181 TVTSAELNLDKMLLERLRSAASKMEKWVKVNKVGVTEDVVNKIQFIWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQV--------HISP 300
           LSRNMDRAREIVEMKTGGMVVWSKKN LV+YRGCNYPL LKHST+ QV         +  
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNGLVVYRGCNYPLGLKHSTEMQVDSSYCSKTQVMQ 300

Query: 301 QNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETD 360
           QNPVK+ETD+ FS   HYE+ LN+SINDNDGEWEE S+ FL+   N QPLSGSLYERETD
Sbjct: 301 QNPVKLETDSDFS-GKHYENDLNQSINDNDGEWEETSTSFLMHQGNFQPLSGSLYERETD 360

Query: 361 RLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLA 420
           RLLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPP+TK NLTDA L HLRKLA
Sbjct: 361 RLLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPFTKPNLTDAELLHLRKLA 420

Query: 421 HSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTL 480
           HSLPTHFVLGRNRKLQGLAA+ILKLWEKS+IAKIALKWGVPNT+NEQMA ELK+LTGGTL
Sbjct: 421 HSLPTHFVLGRNRKLQGLAAAILKLWEKSLIAKIALKWGVPNTNNEQMACELKSLTGGTL 480

Query: 481 LLRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEER 540
           LLRNKF+IILYRG DFLPVGVA+SIIQRE+ELQRWQLHEENSRLKASE FCFDT NMEER
Sbjct: 481 LLRNKFLIILYRGKDFLPVGVANSIIQREMELQRWQLHEENSRLKASELFCFDTGNMEER 540

Query: 541 GKAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTK 600
            KAGTLSDFKDITV Y+DLSTGSTES+LQA+AEKGKIIR LRMQERRL  LNFKVEKS K
Sbjct: 541 AKAGTLSDFKDITVEYQDLSTGSTESKLQADAEKGKIIRELRMQERRLSNLNFKVEKSIK 600

Query: 601 ELTKLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635
           ELTKLNASWR VEPDADQE ITNEERICFRKMGLKMDSCLTLG
Sbjct: 601 ELTKLNASWRHVEPDADQEFITNEERICFRKMGLKMDSCLTLG 636

BLAST of Cucsat.G4812.T13 vs. ExPASy TrEMBL
Match: A0A0A0LSY1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G404830 PE=4 SV=1)

HSP 1 Score: 1256 bits (3251), Expect = 0.0
Identity = 632/635 (99.53%), Postives = 633/635 (99.69%), Query Frame = 0

Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL
Sbjct: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRMKLEEVEFG FDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGGFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP
Sbjct: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISPQNPVKVET 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLKHSTKKQVHISPQNPVKVET
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKHSTKKQVHISPQNPVKVET 300

Query: 301 DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP 360
           DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP
Sbjct: 301 DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP 360

Query: 361 RFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV 420
           RFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV
Sbjct: 361 RFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV 420

Query: 421 LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLLRNKFVI 480
           LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLLRNKFVI
Sbjct: 421 LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLLRNKFVI 480

Query: 481 ILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERGKAGTLSD 540
           ILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERGKAGTLSD
Sbjct: 481 ILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERGKAGTLSD 540

Query: 541 FKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKELTKLNAS 600
           FKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKELTKLNAS
Sbjct: 541 FKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKELTKLNAS 600

Query: 601 WRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635
           WRRVEPDADQELITNEERICFRKMGLKMDSCLTLG
Sbjct: 601 WRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635

BLAST of Cucsat.G4812.T13 vs. ExPASy TrEMBL
Match: A0A5D3C8J4 (Chloroplastic group IIA intron splicing facilitator CRS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G001730 PE=4 SV=1)

HSP 1 Score: 1134 bits (2932), Expect = 0.0
Identity = 579/642 (90.19%), Postives = 599/642 (93.30%), Query Frame = 0

Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSS LP  LIPPSFRSHS LLHLSTH+PISAT TPSQSSVL EPPSISNAA+NL
Sbjct: 1   MLPTTSFSSPLPTILIPPSFRSHSSLLHLSTHHPISATFTPSQSSVLSEPPSISNAALNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQ QQ  EEGVDPANPKRRNGSD +G DKCSRALGD GI+KTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQTQQ--EEGVDPANPKRRNGSDRNGGDKCSRALGDRGIEKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRM LEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIV RRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMNLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVFRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           T+TSAELNLDR+LLERLK +ASKMEKWVKVNKVGVTQDVVN IQ  WERNELAMLKFDVP
Sbjct: 181 TMTSAELNLDRLLLERLKAKASKMEKWVKVNKVGVTQDVVNNIQLKWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHIS-------PQ 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLK STK QV  S        Q
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKPSTKMQVDSSCSKTQVLQQ 300

Query: 301 NPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDR 360
           NPVKVETDTH SLSGHYESGL++S ND+DGEWEEASSFFL+RH NLQPLSGSLYERETDR
Sbjct: 301 NPVKVETDTHSSLSGHYESGLDQSRNDDDGEWEEASSFFLMRHGNLQPLSGSLYERETDR 360

Query: 361 LLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420
           LLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH
Sbjct: 361 LLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420

Query: 421 SLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLL 480
           SLPTHFVLGRNR LQGLA +ILKLWEKSMIAKIALKWGVPNT+NEQMA ELK LTGGTLL
Sbjct: 421 SLPTHFVLGRNRGLQGLAGAILKLWEKSMIAKIALKWGVPNTNNEQMAFELKKLTGGTLL 480

Query: 481 LRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERG 540
           LRNKF+IILYRGNDFLPVGVADSIIQRE+ELQRWQLHEENSRLKASEFFCFDT NMEERG
Sbjct: 481 LRNKFLIILYRGNDFLPVGVADSIIQRELELQRWQLHEENSRLKASEFFCFDTGNMEERG 540

Query: 541 KAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKE 600
           KAGTLSDFKD+TV YEDLST STES+LQAEA++GKIIR LRMQERRLKILNFKVEKSTKE
Sbjct: 541 KAGTLSDFKDVTVEYEDLSTESTESKLQAEAKRGKIIRELRMQERRLKILNFKVEKSTKE 600

Query: 601 LTKLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635
           LTKLNASW RVEPDADQELITNEERICFRKMGLKMDSCLTLG
Sbjct: 601 LTKLNASWSRVEPDADQELITNEERICFRKMGLKMDSCLTLG 640

BLAST of Cucsat.G4812.T13 vs. ExPASy TrEMBL
Match: A0A1S3B2K3 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103485442 PE=4 SV=1)

HSP 1 Score: 1134 bits (2932), Expect = 0.0
Identity = 579/642 (90.19%), Postives = 599/642 (93.30%), Query Frame = 0

Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSS LP  LIPPSFRSHS LLHLSTH+PISAT TPSQSSVL EPPSISNAA+NL
Sbjct: 1   MLPTTSFSSPLPTILIPPSFRSHSSLLHLSTHHPISATFTPSQSSVLSEPPSISNAALNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQ QQ  EEGVDPANPKRRNGSD +G DKCSRALGD GI+KTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQTQQ--EEGVDPANPKRRNGSDRNGGDKCSRALGDRGIEKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRM LEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIV RRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMNLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVFRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           T+TSAELNLDR+LLERLK +ASKMEKWVKVNKVGVTQDVVN IQ  WERNELAMLKFDVP
Sbjct: 181 TMTSAELNLDRLLLERLKAKASKMEKWVKVNKVGVTQDVVNNIQLKWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHIS-------PQ 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLK STK QV  S        Q
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKPSTKMQVDSSCSKTQVLQQ 300

Query: 301 NPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDR 360
           NPVKVETDTH SLSGHYESGL++S ND+DGEWEEASSFFL+RH NLQPLSGSLYERETDR
Sbjct: 301 NPVKVETDTHSSLSGHYESGLDQSRNDDDGEWEEASSFFLMRHGNLQPLSGSLYERETDR 360

Query: 361 LLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420
           LLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH
Sbjct: 361 LLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420

Query: 421 SLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLL 480
           SLPTHFVLGRNR LQGLA +ILKLWEKSMIAKIALKWGVPNT+NEQMA ELK LTGGTLL
Sbjct: 421 SLPTHFVLGRNRGLQGLAGAILKLWEKSMIAKIALKWGVPNTNNEQMAFELKKLTGGTLL 480

Query: 481 LRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERG 540
           LRNKF+IILYRGNDFLPVGVADSIIQRE+ELQRWQLHEENSRLKASEFFCFDT NMEERG
Sbjct: 481 LRNKFLIILYRGNDFLPVGVADSIIQRELELQRWQLHEENSRLKASEFFCFDTGNMEERG 540

Query: 541 KAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKE 600
           KAGTLSDFKD+TV YEDLST STES+LQAEA++GKIIR LRMQERRLKILNFKVEKSTKE
Sbjct: 541 KAGTLSDFKDVTVEYEDLSTESTESKLQAEAKRGKIIRELRMQERRLKILNFKVEKSTKE 600

Query: 601 LTKLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635
           LTKLNASW RVEPDADQELITNEERICFRKMGLKMDSCLTLG
Sbjct: 601 LTKLNASWSRVEPDADQELITNEERICFRKMGLKMDSCLTLG 640

BLAST of Cucsat.G4812.T13 vs. ExPASy TrEMBL
Match: A0A6J1JY40 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489335 PE=4 SV=1)

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 505/641 (78.78%), Postives = 541/641 (84.40%), Query Frame = 0

Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNP---ISATSTPSQSSVLPEPPSISNAA 60
           ML TT FS +LPR L PPSF S S  LHLS+H P   I ATST SQSS+L EP S SNAA
Sbjct: 1   MLATTCFSPALPRILFPPSFLSDSTPLHLSSHKPRAPIPATSTGSQSSLLSEPSSDSNAA 60

Query: 61  VNLRTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYA 120
           +N RTAPW ++PL+LQP   EEEGVD A P+R NGSDG G  K  RAL D    KTGKYA
Sbjct: 61  INHRTAPWTESPLYLQP--LEEEGVDLAKPERSNGSDGHGGKKRYRALADRRNVKTGKYA 120

Query: 121 MRRIAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRM 180
           MRRIAKSI KL++NGD GET MKLEEVEFGDF LEGF+ES TR RMPWEK DDGIVLRR 
Sbjct: 121 MRRIAKSIEKLQKNGDSGETGMKLEEVEFGDFYLEGFDESRTRGRMPWEK-DDGIVLRRP 180

Query: 181 KKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKF 240
           KKKTVTSAEL+LD+VLLERL+GEASKME WVKVNK+GVTQDVVN+I F WE NELAMLKF
Sbjct: 181 KKKTVTSAELSLDKVLLERLRGEASKMENWVKVNKIGVTQDVVNKIHFTWETNELAMLKF 240

Query: 241 DVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISP---QN 300
           DVPLSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPL LK  TK QV  SP   + 
Sbjct: 241 DVPLSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLGLKLRTKMQVD-SPYCSRT 300

Query: 301 PVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRL 360
           P  +ETDT FSL  H E G NRSIN+NDGEW EAS+ FL         SGSLYERETDRL
Sbjct: 301 PF-LETDTDFSLGEHDEGGSNRSINNNDGEWGEASTSFL---------SGSLYERETDRL 360

Query: 361 LDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHS 420
           LDDLGPRFIDWWMHKPLPVDADML EVVPGYMPP+RRCPP+T   LTDA L +LRKLAH 
Sbjct: 361 LDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPYRRCPPFTNPKLTDAELLYLRKLAHP 420

Query: 421 LPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLL 480
           LPTHFVLGRNRKLQGLAA+ILKLWEKS+IAKIALKWGVPNT+NEQMA ELK+LTGGTLLL
Sbjct: 421 LPTHFVLGRNRKLQGLAAAILKLWEKSLIAKIALKWGVPNTNNEQMAYELKSLTGGTLLL 480

Query: 481 RNKFVIILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERGK 540
           RNKF IILYRG DFLPVG+A SIIQRE ELQRWQLHEENSRLKA E FCFDT N+EER K
Sbjct: 481 RNKFFIILYRGKDFLPVGLASSIIQRETELQRWQLHEENSRLKARELFCFDTGNLEERAK 540

Query: 541 AGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKEL 600
           AGTLSDFKDIT+ YEDL+TGSTES+LQA+AEKGKIIR L+ QERRL IL+FKVEKSTKEL
Sbjct: 541 AGTLSDFKDITMEYEDLTTGSTESKLQADAEKGKIIRELKKQERRLSILDFKVEKSTKEL 600

Query: 601 TKLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635
           TKLNAS R VEPDADQELITNEERICFRKMGLKM SCLTLG
Sbjct: 601 TKLNASCRHVEPDADQELITNEERICFRKMGLKMHSCLTLG 627

BLAST of Cucsat.G4812.T13 vs. ExPASy TrEMBL
Match: A0A6J1FHM9 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445437 PE=4 SV=1)

HSP 1 Score: 939 bits (2426), Expect = 0.0
Identity = 506/641 (78.94%), Postives = 541/641 (84.40%), Query Frame = 0

Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNP---ISATSTPSQSSVLPEPPSISNAA 60
           ML TT FS +LP  L PPSFRS S  LHLS+H P   I ATST SQSS+L  P S SNAA
Sbjct: 1   MLATTCFSPALPTILFPPSFRSDSTPLHLSSHKPRAPIPATSTGSQSSLLSGPSSDSNAA 60

Query: 61  VNLRTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYA 120
           +NLRTAPW KAPL+LQP  QEEEGVD ANP+R NGSDG G  K  RAL D    KTGKYA
Sbjct: 61  INLRTAPWTKAPLYLQP--QEEEGVDLANPERSNGSDGHGDKKRYRALADRRNVKTGKYA 120

Query: 121 MRRIAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRM 180
           MRRIAKSI KL++NGD GET MKLEEVEFGDF LEGF+ES TR RMPWEK DDGIVLRR 
Sbjct: 121 MRRIAKSIEKLQKNGDSGETGMKLEEVEFGDFYLEGFDESRTRGRMPWEK-DDGIVLRRP 180

Query: 181 KKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKF 240
           KKKTVTSAEL+LD+VLLERL+GEASK+EKWVKVNK+GVTQDVVN+I F WE NELAMLKF
Sbjct: 181 KKKTVTSAELSLDKVLLERLRGEASKIEKWVKVNKIGVTQDVVNKIHFTWETNELAMLKF 240

Query: 241 DVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISP---QN 300
           DVPL RNMDRAREIVEMKTGGMVVWSKKNA V+YRGCNYPL LK STK QV  SP   + 
Sbjct: 241 DVPLCRNMDRAREIVEMKTGGMVVWSKKNAHVVYRGCNYPLGLKLSTKMQVD-SPYCSRT 300

Query: 301 PVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRL 360
           P  +ETDT FSL    E G NRSIN+N  EW EAS+ FL         SGSLYERETDRL
Sbjct: 301 PF-LETDTDFSLDEPDEGGSNRSINNNVDEWGEASTSFL---------SGSLYERETDRL 360

Query: 361 LDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHS 420
           LDDLGPRFIDWWMHKPLPVDADML EVVPGYMPP+RRCPP+T   LTDA L +LRKLAH 
Sbjct: 361 LDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPYRRCPPFTNPKLTDAELLYLRKLAHP 420

Query: 421 LPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLL 480
           LPTHFVLGRN KLQGLAA+ILKLWEKS+IAKIALKWGVPNT+NEQMA ELK+LTGGTLLL
Sbjct: 421 LPTHFVLGRNTKLQGLAAAILKLWEKSLIAKIALKWGVPNTNNEQMACELKSLTGGTLLL 480

Query: 481 RNKFVIILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCFDTENMEERGK 540
           RNKF IILYRG DFLPVGVA+SIIQRE ELQRWQLHEENSRLKASE FCFDT N+EER K
Sbjct: 481 RNKFFIILYRGKDFLPVGVANSIIQRETELQRWQLHEENSRLKASELFCFDTGNLEERAK 540

Query: 541 AGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKEL 600
           AGTLSDFKDIT+ YEDL+TGSTES+LQA+AEKGKIIR L+ QERRL IL+FKVEKSTKEL
Sbjct: 541 AGTLSDFKDITMEYEDLTTGSTESKLQADAEKGKIIRELKKQERRLSILDFKVEKSTKEL 600

Query: 601 TKLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 635
           TKLNAS R VEPDADQELITNEERICFRKMGLKM SCLTLG
Sbjct: 601 TKLNASCRHVEPDADQELITNEERICFRKMGLKMHSCLTLG 627

BLAST of Cucsat.G4812.T13 vs. TAIR 10
Match: AT5G16180.1 (ortholog of maize chloroplast splicing factor CRS1 )

HSP 1 Score: 449.9 bits (1156), Expect = 3.4e-126
Identity = 274/608 (45.07%), Postives = 364/608 (59.87%), Query Frame = 0

Query: 33  NPISATSTPSQSSVLPEPPSISNAAVNLRTAPWMKAPLHLQPQQQEEEGVDPANPKRRNG 92
           N  S  +  SQ     E   +S+AA+ + TAPWMK PL L+P        +  + K+RN 
Sbjct: 56  NSSSRRAKSSQFDQFRENRGVSDAAIKVPTAPWMKGPLLLRPD-------EILDTKKRN- 115

Query: 93  SDGSGRDKCSRALGDSGIDKTGKYAMRRIAKSIGKLRRNGDLGETRM-KLEEVEF-GDFD 152
                 +K  +AL        GK AM++I +++ KL  + D  ET+M  L E E+ G  +
Sbjct: 116 KPRKVEEKTFKALNRRESGVRGKKAMKKIVRNVEKLDEDSDSEETQMDDLSEFEYLGRIE 175

Query: 153 LEGFEESGTRRRMPWEKDDDGIVLRRMKKKTV-TSAELNLDRVLLERLKGEASKMEKWVK 212
            +   +     +MPWE++++  +LRRMKK++V T+AEL LD  LL RL+ EASKM KWV 
Sbjct: 176 EKVESKDRFGGKMPWEREEERFILRRMKKESVPTTAELILDEGLLNRLRREASKMRKWVN 235

Query: 213 VNKVGVTQDVVNQIQFMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALV 272
           V K GVT+ VVN+I+ MW+ NELAM++FDVPL RNM+RA+EI+EMKTGG+VV SKK  LV
Sbjct: 236 VRKAGVTELVVNKIKSMWKLNELAMVRFDVPLCRNMERAQEIIEMKTGGLVVLSKKEFLV 295

Query: 273 IYRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEAS 332
           +YRG                                    Y S                 
Sbjct: 296 VYRG---------------------------------GPSYSS----------------- 355

Query: 333 SFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPF 392
                  E    +S SLYERE DRLLD LGPR++DWWM +P PVDAD+L EVV GYM P 
Sbjct: 356 -------EGQDEISSSLYEREADRLLDGLGPRYMDWWMRRPFPVDADLLPEVVNGYMTPS 415

Query: 393 RRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALK 452
           RRCPP T+  LTD  L +LR +A  LP HFVLGRN  LQGLA++I+KLWEK +IAKIA+K
Sbjct: 416 RRCPPNTRAKLTDEELTYLRNIAQPLPFHFVLGRNYGLQGLASAIVKLWEKCIIAKIAIK 475

Query: 453 WGVPNTDNEQMALELKNLTGGTLLLRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQL 512
           WG  NT+NE+MA EL+ LTGG L+LRNK++I+LYRG DFL   VAD +  RE  L R+Q 
Sbjct: 476 WGALNTNNEEMADELRYLTGGVLILRNKYLIVLYRGKDFLSDEVADLVEDRERLLSRYQH 535

Query: 513 HEENSRLKASEFFCFDT--ENMEERGKAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKG 572
            EE  R    E     T  + ++E  K+GTL +F+++   + ++        L+ EAEK 
Sbjct: 536 FEETKRESDIELLEVVTNGKQLKETNKSGTLLEFQELQRKFGEMD----PRNLETEAEKA 594

Query: 573 KIIRGLRMQERRLKILNFKVEKSTKELTKLNASWRRVEPDADQELITNEERICFRKMGLK 632
           ++ + L+ QE +L IL  K+EKS  EL KLN+ W+  E D D E++TNEER C R++GLK
Sbjct: 596 RLEKELKSQEHKLSILKSKIEKSNMELFKLNSLWKPSEGDDDIEILTNEERECLRRIGLK 594

Query: 633 MDSCLTLG 636
           M+S L LG
Sbjct: 656 MNSSLVLG 594

BLAST of Cucsat.G4812.T13 vs. TAIR 10
Match: AT3G18390.1 (CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 288.9 bits (738), Expect = 1.0e-77
Identity = 183/487 (37.58%), Postives = 276/487 (56.67%), Query Frame = 0

Query: 163 MPWEKDDD-----------GI-VLRRMKKKTVTSAELNLDRVLLERLKGEASKMEKWVKV 222
           +PWEK+++           G+ V+++ + +  + AEL ++   L RL+ +   +   + +
Sbjct: 205 LPWEKNEEEQAAERVVGEGGVAVMQKRRARAPSLAELTVEDSELRRLRRDGMYLRVRINI 264

Query: 223 NKVGVTQDVVNQIQFMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALVI 282
            K G+TQ V+ +I   W + EL  LKF   L+R+M  A EIVE +TGGMV+W   + +V+
Sbjct: 265 PKAGLTQAVMEKIYDTWRKEELVRLKFHEVLARDMKTAHEIVERRTGGMVIWRAGSVMVV 324

Query: 283 YRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDG-EWEEAS 342
           YRG +Y        K    IS Q     ET     +S   +   N   N +     ++  
Sbjct: 325 YRGLDY--------KGPPVISNQMAGPKETLFVPDVSSAGDEATNAKDNQSAPLVIKDPI 384

Query: 343 SFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPF 402
               IR EN+     +  E E + LLD LGPRF +WW    LPVDAD+L   +PGY  PF
Sbjct: 385 IKNPIRKENM-----TEEEVEFNSLLDSLGPRFQEWWGTGVLPVDADLLPPTIPGYKTPF 444

Query: 403 RRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALK 462
           R  P   + NLT+A + +LRK+  +LP HF LGRNR  QGLAA+IL++WEKS+IAKIA+K
Sbjct: 445 RLLPTGMRSNLTNAEMTNLRKIGKTLPCHFALGRNRNHQGLAAAILQIWEKSLIAKIAVK 504

Query: 463 WGVPNTDNEQMALELKNLTGGTLLLRNKFVIILYRGNDFLPVGVADSIIQREVELQRWQL 522
            G+ NT+N+ MA E+K LTGG LLLRNK+ I++YRG DFLP  VA ++ +R+ EL + ++
Sbjct: 505 RGIQNTNNKLMADEVKTLTGGVLLLRNKYYIVIYRGKDFLPSSVAATLAERQ-ELTK-EI 564

Query: 523 HEENSRLKASEFFCFDTENMEERGKAGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKI 582
            +   R++  E         +   +AGTL++F +    +    T     ++  EA +   
Sbjct: 565 QDVEERVRNREIEAVQPVGDKVPAEAGTLAEFYEAQARWGKEITPDHREKMIEEASRVAN 624

Query: 583 IRGLRMQERRLKILNFKVEKSTKELTKLNASWRRVEPDADQELITNEERICFRKMGLKMD 637
            R ++  + +L +   K +++ K L+K+ AS     PD DQE+I+ EER  FRK+GLKM 
Sbjct: 625 ARVVKRIQHKLNLAQSKFQRAEKLLSKIEASMIPNGPDYDQEVISEEERAMFRKVGLKMK 676

BLAST of Cucsat.G4812.T13 vs. TAIR 10
Match: AT3G23070.1 (CRM family member 3A )

HSP 1 Score: 261.9 bits (668), Expect = 1.3e-69
Identity = 167/460 (36.30%), Postives = 247/460 (53.70%), Query Frame = 0

Query: 178 KKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKF 237
           K+   + AE+ L    L RL+    +    +++   GVTQ  V+ I+  W+  E+  LK 
Sbjct: 210 KENRYSLAEMTLPESELRRLRNLTFRTASKMRIRGGGVTQVAVDAIKEKWKSAEIVRLKI 269

Query: 238 DVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISPQNPVK 297
           +   + NM +  EI+E KTGG+V+W    ++ +YRG +Y L      K++         +
Sbjct: 270 EGASALNMRKMHEILEKKTGGLVIWRSGTSISLYRGVSYELPSGKWNKQR---------R 329

Query: 298 VETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDD 357
            ET     +  H E+      +D      +         +  Q      YE E D LLDD
Sbjct: 330 EETPPEAVIENHDETTTMVDKSDEKVHLPQLEQETTSVEKKDQTSPVVEYEDELDELLDD 389

Query: 358 LGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPT 417
           LGPRF+DW    PLPVDAD+L   +P Y PPFR  P   + +L       LR+LA S+P 
Sbjct: 390 LGPRFMDWPGDNPLPVDADLLPGAIPDYEPPFRVLPYGVRSSLGPKEATALRRLARSIPP 449

Query: 418 HFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLLRNK 477
           HF LGR+R+LQGLA ++++LWEKSM+AKIA+K GV +T +E+MA +LK LTGG +L RNK
Sbjct: 450 HFALGRSRQLQGLATAMVRLWEKSMLAKIAIKRGVQSTTSERMAEDLKKLTGGIMLSRNK 509

Query: 478 FVIILYRGNDFLPVGVADSIIQREVELQRWQLHEENSRLKASEFFCF-DTENMEERGKAG 537
             ++ YRG +FL   VAD+++++E  ++  Q  EE +RL+ S       TE   +   AG
Sbjct: 510 DFLVFYRGKNFLSREVADALVEQERFVRTLQDEEEQARLRGSSALIVPSTEPANKLVSAG 569

Query: 538 TLSDFKDITVGY-EDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKELT 597
           TL +  D T  + ++L        ++ E E  +    +R  ER+L     K+ K+ + L 
Sbjct: 570 TLGETLDATGKWGKNLDDDDHSDEVKQEVEILRHENLVRKLERKLAFAERKLLKAERGLA 629

Query: 598 KLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 636
           K+    +  E   D E IT+EER  FRK+GLKM + L LG
Sbjct: 630 KVEVCLKPAEQREDPESITDEERFMFRKLGLKMKAFLLLG 660

BLAST of Cucsat.G4812.T13 vs. TAIR 10
Match: AT4G29750.1 (CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 261.5 bits (667), Expect = 1.7e-69
Identity = 171/463 (36.93%), Postives = 250/463 (54.00%), Query Frame = 0

Query: 176 RMKKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAML 235
           R +K     AE  +    L+RL+  A +M + VKV   G+TQ +V  I   WE +E+  L
Sbjct: 231 RTRKSNTVEAERIVPEHELKRLRNVALRMVERVKVGSAGITQALVEAIHEKWEVDEVVKL 290

Query: 236 KFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISPQNP 295
           KF  P S NM R  E++E KTGG+V+W   +++V+YRG +Y L    +  K      QN 
Sbjct: 291 KFSEPYSLNMKRTHEVLEKKTGGLVIWRSGSSVVLYRGISYKLKCVQTFIK------QNN 350

Query: 296 VKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLL 355
           ++   + H            RS+   D   E+ +    +  E L  L       E + LL
Sbjct: 351 LEANPEIH------------RSVEARDYVQEDGNYPKNVPKEQLSELC------ELNDLL 410

Query: 356 DDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSL 415
           D++GPRF DW    P PVDAD+L   V GY  PFR  P   K  L++  +  +R+LA + 
Sbjct: 411 DEVGPRFHDWTGCAPFPVDADLLPGYVEGYRCPFRILPQGVKPCLSNTEMTEMRRLARTS 470

Query: 416 PTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKNLTGGTLLLR 475
           P HF LGR+R+LQGLA +++KLW KS IAKIA+K GV NT NE+MA ELK LT G L+ R
Sbjct: 471 PPHFALGRSRELQGLAKAMVKLWAKSAIAKIAIKRGVENTRNERMAEELKRLTRGVLVSR 530

Query: 476 NKFVIILYRGNDFLPVGVADSIIQREVEL-QRWQLHEENSRLKASEFFCFDTENMEERGK 535
           NK  I+ YRGNDF+P  VA+++ +R+ E+ +  Q  E+ +R  AS      ++    + +
Sbjct: 531 NKEYIVFYRGNDFMPPAVAEALTERQKEITEVLQAKEDQAREMASTRATLTSQAKSPKTQ 590

Query: 536 --AGTLSDFKDITVGYEDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTK 595
             AGTL++    +  +   ++      L+ E+   K    +R  E RL     K+ ++ +
Sbjct: 591 LLAGTLAETIAASSRWAPNASSVDIEELKRESASIKRAALIRDLELRLLYGKQKLRRAER 650

Query: 596 ELTKLNASWRRVEPDADQELITNEERICFRKMGLKMDSCLTLG 636
           +L K+       E   D E+IT EER+ +RK+GL MD  L LG
Sbjct: 651 DLAKVQKDLDPSELPTDSEIITEEERLLYRKIGLSMDPFLLLG 669

BLAST of Cucsat.G4812.T13 vs. TAIR 10
Match: AT4G14510.1 (CRM family member 3B )

HSP 1 Score: 246.9 bits (629), Expect = 4.4e-65
Identity = 171/518 (33.01%), Postives = 265/518 (51.16%), Query Frame = 0

Query: 162 RMPWEKDDDGIVLRRMKKKTVTS-------------AELNLDRVLLERLKGEASKMEKWV 221
           + PWEK      +  M+KK + +             AE+ L    L RL+    + +  +
Sbjct: 188 KFPWEK------VSSMEKKELVNGEWTAKKESRYSLAEMTLSEFELNRLRNVMFRTKSKM 247

Query: 222 KVNKVGVTQDVVNQIQFMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNAL 281
           +V   GVTQ VV+ IQ  W+ +E+  LK +   + NM R  EI+E KTGG+V+W    ++
Sbjct: 248 RVTGAGVTQAVVDAIQEKWKGSEIVRLKIEGSSALNMRRMHEILERKTGGLVIWRSGTSI 307

Query: 282 VI--YRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWE 341
            +  Y+G +      +  K+    + + P  + T T           +++S+   +    
Sbjct: 308 ALYNYKGGSNRDGSGNMNKQVYRRAERLPSSLPTST-----------VDQSVQLVNLPQL 367

Query: 342 EASSFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYM 401
           E     +   +   P     YE E + LL+ LGPR+ DW    PLPVDAD+L  +VPGY 
Sbjct: 368 EKEPTVVGNKDRTSPQEVE-YEDEINELLEGLGPRYTDWQGGYPLPVDADLLPGIVPGYE 427

Query: 402 PPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKI 461
           PPFR  P   +  L       LR++A  LP HF LGR+R+LQGLA +++KLW+KS+IAK+
Sbjct: 428 PPFRALPYGVRSTLGTKEATSLRRIATVLPPHFALGRSRQLQGLATAMVKLWQKSLIAKV 487

Query: 462 ALKWGVPNTDNEQMALELKNLTGGTLLLRNKFVIILYRGNDFLPVGVADSIIQREVELQR 521
           ALK GV  T +E+MA ++K LTGG LL RNK  ++ YRG  FL + V ++++++E+ ++ 
Sbjct: 488 ALKRGVQLTTSERMAEDIKRLTGGMLLSRNKDFLVFYRGKSFLSLEVGEALMEKEMLVRT 547

Query: 522 WQLHEENSRLKASEFFCFDT-----------ENMEERGK-----------------AGTL 581
            Q  EE +RL+AS      +           ++ EE+ +                 AGTL
Sbjct: 548 LQDEEEQARLRASSALVVPSIKANQQLARTLQDKEEQARPSALVLPSTKANQNLVSAGTL 607

Query: 582 SDFKDITVGY-EDLSTGSTESRLQAEAEKGKIIRGLRMQERRLKILNFKVEKSTKELTKL 636
            +  D T  + ++L        ++ E EK +  + +R  ER+L     K+ K+ + L K+
Sbjct: 608 GETLDATGKWGKNLDNDDHVEEMKQEVEKVRSAKLVRKLERKLAFAEKKLLKAERALAKV 667