Cucsat.G4812.T12 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G4812.T12
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic
Location	ctg1227: 4036300 .. 4041485 (+)
RNA-Seq Expression	Cucsat.G4812.T12
Synteny	Cucsat.G4812.T12

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTTTTATCTCAACTAATAAATTAAGAGAGAGAGAGAAAAAAAGGCTGAAGCTACGTGGCGAGCAATGGCGGCATCTGACCGGATGACAGAGAAGTGATCGGCAGCCGCATCTTCTCCTCCAAAATAGAAGGGAGCACAGTGACATTCCATTAACTGATTACTCCATCCTACTCTGTTATGGAAATACTACGCCATTGATACACCTCAACAAACTATGCTTCCCACAACTTCCTTCTCCTCATCTCTTCCAAGGAGTCTTATTCCCCCATCCTTTCGATCACACTCTCCTCTCTTACACCTCTCCACCCACAACCCCATTTCCGCCACTTCTACACCGTCACAATCCTCTGTTCTACCCGAGCCGCCTTCCATTTCCAATGCCGCCGTTAACCTCCGTACAGCGCCATGGATGAAGGCTCCTCTCCACCTTCAACCCCAACAACAAGAAGAAGAAGGCGTGGATCCCGCAAACCCCAAAAGAAGAAATGGGTCCGATGGAAGTGGGCGCGATAAGTGTTCTAGGGCTTTAGGTGATAGTGGAATTGATAAAACTGGGAAATACGCGATGCGACGAATTGCTAAAAGCATTGGAAAGCTTCGGAGGAATGGGGATTTGGGAGAAACCCGAATGAAATTGGAGGAGGTTGAATTTGGGGATTTTGATTTGGAGGGTTTTGAGGAGTCTGGAACTCGCCGGAGAATGCCATGGGAGAAAGATGATGATGGGATTGTTTTGCGGAGGATGAAGAAGAAAACAGTGACTTCAGCGGAGTTGAATCTTGATAGAGTGTTGCTTGAAAGGTTGAAGGGCGAGGCTTCAAAAATGGAGAAATGGGTGAAGGTCAATAAAGTTGGAGTTACTCAAGATGTTGTGAATCAGATACAATTCATGTGGGAGAGAAATGAGCTTGCTATGCTGAAATTCGATGTGCCCTTGAGCCGCAACATGGACAGAGCACGAGAAATTGTTGAGGTAAGGTTGGTTTTGTTTAACTTTCTCTTTTGTATTTCAAGTATTTTGTTATTTGAAGTCCTTTATATTTTGTAATTCCTATTGCGAGTACGAATCCCTTTGCAATATACCATTGAGAAGTGTTTTGTTAAAAACAACACAAGTAAATAGATATGCTCTAATGTTGCTTACTTTATTTGGAAAATTACTGCTTTATGTTGCTACTCATTTTTGGACTGTCAGATTGATAGTAATCATATTATGCGTAGGATGTCTTTTTATGGCTATGGTTTGTCTTTCTTAATCTTTTCTATTGTACAGATGAAAACCGGAGGTATGGTAGTCTGGAGTAAGAAGAATGCTCTTGTTATTTACCGGGGTTGCAATTATCCACTGAATTTGAAACATAGTACGAAAAAGCAAGTACATATCTCGCCACAGAATCCAGTAAAGGTGGAAACGGACACTCATTTTTCGCTCAGCGGACATTATGAAAGTGGTTTAAATCGAAGTATAAATGACAATGATGGTGAATGGGAAGAAGCTTCTAGTTTTTTCTTGATCCGTCATGAAAATCTCCAACCATTGAGTGGATCACTCTATGAGAGGGAAACTGACAGGCTATTGGATGATTTGGGACCCCGCTTTATTGACTGGTGGATGCACAAACCTCTACCGGTTGATGCTGACATGCTTCAAGAGGTGGTTCCTGGATATATGCCTCCATTTAGGCGTTGTCCACCCTATACTAAACAAAATCTTACAGATGCTGGACTACAACATCTGAGGAAGCTTGCACATTCCTTACCTACTCATTTTGTCCTCGGTATAAATTTTGTTTTTTTCTTCGTTCAGTAAAACTTTTCTTGAGCTCTTCAGTTGAAATGTTAGTCAGAGATAGGATGAAGGTATAATCAGGGTAGAAGGTTTTGAATAGCTGTTTTTCTTTGCTTACATTTTATGGGAAGTCACGTCAAAACTGAAGCAATTTAAGTGAAGGTCTCAAGCAAGCAACACAATAACATGAACACCTTAACCTGTTTGTTTCTTTTTTTTTTTCCTTAATTATTTGGTCTAGTACAATTTCCTCTAATCTAACAGTGTAGAAATGTCTTGATATAATTTTAAAAGGGCGGTTAATGATCAAATGAGCTGAAACAAATCTGATATTTACTCTCTATTCGCCTTCATAAACTATTCTGTAGGGTCCACGGTTTAAACTTCACCCCCAATCAACATGCATTTTCATTTGTGGATAAACAATATTTTGTGCTCTGCTTATGATGTTGTGTGTGCATGTTCTTAGCTAGCTACATACAGACTCTGCTATATTATGGCTGGATTAAATATTATCCACGTAGTTTATTTTGTATTTAATCTTCTTCTGCATGTACTTGTATGTTCCACTAATTTTTTAATTTCCAAATATTTGAAGGGAGAAACAGAAAACTTCAAGGGTTGGCTGCGTCCATCTTAAAGTTGTGGGAGAAAAGTATGATAGCTAAGATTGCTTTGAAGTGGGGGGTTCCAAATACAGACAATGAGCAGATGGCATTGGAACTTAAGGCAAGTTTTCTAGAAATAATATTTGTTATACAACTGTATAAGCTGTGAGACCTTGAACCTGGAATATTAAAGACATTGTCCTTAAATGAACGTCAATCTGGCTAAAGTGGCAAATTATTGTCTGTATCTTATGTCTTTGAACATCCAAATTGCGTGAAAGTTGCTAATGAGATTTTATTGTTAATCCCTTTTTTGATAGATAATAACATTTTCATGAAAGGCAAAGAAAATTTAAAGCAGGAAAAAGGAAGTAACCCCTCCCAATAAATGAGAGAGCTAAGCAAAACACTCTAATGCGAGCATCTCTAAAAGCTTACAGAAATTCTTTCTTTGGAGATCCACTCAACAAATTCAGTTGATGGTAGAGCACATATGTATCTGAAGTGTGGATGTTTTAACTTATTTTTTGTACGGACTGGAGACATCTGGTTATTGCCCTAAACTATGCTTATGGTGTTTGTGTCCTCTGCTACAATTTAGTGTGTTATGCACGCAAGCATAATTTGTTATTCCATTAAATAACTATTACTAGTTCAGAATCTCACAGGAGGAACATTGCTGTTGCGCAATAAATTTGTCATTATTCTTTATCGAGGCAACGACTTCCTACCTGTTGGAGTTGCTGATTCAATAATTCAAAGAGAAGTAGAGCTCCAAAGATGGCAACTTCACGAGGAAAATTCACGGCTGAAAGCCAGTGAATTTTTCTGTTTTGACACTGAGAACATGGAAGAGAGAGGGAAGGCTGGAACTCTATCAGATTTTAAGGATATCACAGTGGGGTATGAAGATTTGTCAACTGGAAGCACAGAATCAAGACTTCAAGCAGAAGCTGAAAAGGGAAAAATAATTAGGGGCCTAAGGATGCAAGAGCGAAGACTTAAAATTGTAAGGTTTACTTTTGTGTGCTAATATCTGAATCGACCATCCTTGTTAAGCTGTTATCACGCACCAGTGCACCACCTGCTGATGTTTTTATCTTATTGGTATTTTGCAGCTTAACTTTAAGGTAGAGAAGTCAACAAAGGAGTTGACGAAGTTGAATGCATCATGGAGGCGTGTTGAGCCAGATGCAGACCAGGAACTTATTACAAATGAGGAGAGAATATGTTTTAGGAAGATGGGTTTGAAGATGGATAGCTGCTTAACACTCGGTATTAGTTCAACTCCCATCCCTTCCATTTAAATTGGACCTTTGTTAGGATGCATTGCCTAGTACCCGAGCTCCTTTAGAATTCTATTGCAAACATAAATTTGCTCTAATTTATTTCGATGAAAACATAAGATTTCCTATTGTTTTGCACTCATGTACATAACTCGGACTAACCTTGGGAAATGAAAAAAATTCAATAGAAGGGTTAAATTGTGGAAAGCTATGTAATAAAAGGTAACCAGTATAGCGTCACTTTTTTGCCCAGGTTTTATTCTTTAGTTTTGATTTTTTGGCGACTTCAGGTAGGCGGGGTGTATTTGATGGTGTCATTGAAGGCTTGCATCAACATTGGAAACACAGAGAGGTAGTAAAGGTGATTACCATGCAGAGAGCTTTTAATCAGGTTAATTACACCGCAAAATTGCTTGAAGCAGAAAGTGGTGGGATTTTAGTATCAGTGGACAAACTAAAAGAAGGCTATGCCATAATTATTTTCCGTGGAAAGAACTATAAACGACCTCTACATTCAGTGTCGAAGAATCTCTTGACTAAAAGAAAAGCATTAAGTAGGTCCTTGGAGATGCAAAGAATTGGAGTAAGTGAAGCCTTTTTGTTTTAAACTGCTGTTCTTTGTTCTGTTAATATAATGCTCAAGTTTTGTGTCAATCTGCCTGCTTCGATTCTGCTAATTTTCATGTTTTCATTTCAGTCACTGAAGTTCTTTGCTAATCAGAGACAGCAAAAAATTTATGAATTACAACACGAACTGGTGAGTATTCAGTCCTCTTTATATTGGAAATGTCTTAATGAGAGGTGTCACATATAAATCTTTTAACATTATTGCAATTTGTATTATATGATATTTCTCTTTGATAATAAAACTGTTCTCTTTTGCCCACGGACGTAGCTAACATTCTATCAATGAACCACATAACATGTGTCAATTTTTATTGTTTATGCTTTTTCGTTTTTTTTATTTGTCGATTTCTTAACACAGGAGAAAGTGCGAGATAGTGAGGAGTAAATTAATCAGAACTAGAGTACAAAGCTTGTCGATAAAAGCACACTTCTTCACTTCTACATTTGAAATGTGGGCGAATTGGGTATGCTAAAGTGATTTAAATATCTAGTTGCATCTCCTCGATCTACTGTTTTTTTTTATTATTATTATACATTTGTTTACTTTTATTGGTACCTTAGGTCTTTGAAGAACGTATCAGATATCCATGTGAATAAAGTTTGTTAGGCAGAGGCCCCATATTCAATAATGGATGCTTTAAAACTGTGAAAATAACAAAGAAGCTAAGATGAAGAGGAAGAAATTCTTTAGATATTGTGGAGCATAAGTGGATATGTCTACTTCGATTCTGGAATAGATACTGTAAGTGAAAAAGAGAGAAAAAAAAAATTGTTTTCCATTTATAAATTGTATTAGAATTAATTATTTTGTATATTGATCAACTATGTTATTCTTTTATATCTAATGTAACATGTACATAAATCAGTGAACTCT

Coding sequence (CDS)

ATGCTTCCCACAACTTCCTTCTCCTCATCTCTTCCAAGGAGTCTTATTCCCCCATCCTTTCGATCACACTCTCCTCTCTTACACCTCTCCACCCACAACCCCATTTCCGCCACTTCTACACCGTCACAATCCTCTGTTCTACCCGAGCCGCCTTCCATTTCCAATGCCGCCGTTAACCTCCGTACAGCGCCATGGATGAAGGCTCCTCTCCACCTTCAACCCCAACAACAAGAAGAAGAAGGCGTGGATCCCGCAAACCCCAAAAGAAGAAATGGGTCCGATGGAAGTGGGCGCGATAAGTGTTCTAGGGCTTTAGGTGATAGTGGAATTGATAAAACTGGGAAATACGCGATGCGACGAATTGCTAAAAGCATTGGAAAGCTTCGGAGGAATGGGGATTTGGGAGAAACCCGAATGAAATTGGAGGAGGTTGAATTTGGGGATTTTGATTTGGAGGGTTTTGAGGAGTCTGGAACTCGCCGGAGAATGCCATGGGAGAAAGATGATGATGGGATTGTTTTGCGGAGGATGAAGAAGAAAACAGTGACTTCAGCGGAGTTGAATCTTGATAGAGTGTTGCTTGAAAGGTTGAAGGGCGAGGCTTCAAAAATGGAGAAATGGGTGAAGGTCAATAAAGTTGGAGTTACTCAAGATGTTGTGAATCAGATACAATTCATGTGGGAGAGAAATGAGCTTGCTATGCTGAAATTCGATGTGCCCTTGAGCCGCAACATGGACAGAGCACGAGAAATTGTTGAGATGAAAACCGGAGGTATGGTAGTCTGGAGTAAGAAGAATGCTCTTGTTATTTACCGGGGTTGCAATTATCCACTGAATTTGAAACATAGTACGAAAAAGCAAGTACATATCTCGCCACAGAATCCAGTAAAGGTGGAAACGGACACTCATTTTTCGCTCAGCGGACATTATGAAAGTGGTTTAAATCGAAGTATAAATGACAATGATGGTGAATGGGAAGAAGCTTCTAGTTTTTTCTTGATCCGTCATGAAAATCTCCAACCATTGAGTGGATCACTCTATGAGAGGGAAACTGACAGGCTATTGGATGATTTGGGACCCCGCTTTATTGACTGGTGGATGCACAAACCTCTACCGGTTGATGCTGACATGCTTCAAGAGGTGGTTCCTGGATATATGCCTCCATTTAGGCGTTGTCCACCCTATACTAAACAAAATCTTACAGATGCTGGACTACAACATCTGAGGAAGCTTGCACATTCCTTACCTACTCATTTTGTCCTCGGGAGAAACAGAAAACTTCAAGGGTTGGCTGCGTCCATCTTAAAGTTGTGGGAGAAAAGTATGATAGCTAAGATTGCTTTGAAGTGGGGGGTTCCAAATACAGACAATGAGCAGATGGCATTGGAACTTAAGGCAAGTTTTCTAGAAATAATATTTGTTATACAACTGTATAAGCTGTGA

Protein sequence

MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNLRTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRRIAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKASFLEIIFVIQLYKL

Homology

[Close]

BLAST of Cucsat.G4812.T12 vs. ExPASy Swiss-Prot
Match: Q9LF10 (Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g16180 PE=3 SV=2)

HSP 1 Score: 324.7 bits (831), Expect = 1.8e-87
Identity = 198/436 (45.41%), Postives = 257/436 (58.94%), Query Frame = 0


Query: 33  NPISATSTPSQSSVLPEPPSISNAAVNLRTAPWMKAPLHLQPQQQEEEGVDPANPKRRNG 92
           N  S  +  SQ     E   +S+AA+ + TAPWMK PL L+P        +  + K+RN 
Sbjct: 56  NSSSRRAKSSQFDQFRENRGVSDAAIKVPTAPWMKGPLLLRPD-------EILDTKKRN- 115

Query: 93  SDGSGRDKCSRALGDSGIDKTGKYAMRRIAKSIGKLRRNGDLGETRM-KLEEVEF-GDFD 152
                 +K  +AL        GK AM++I +++ KL  + D  ET+M  L E E+ G  +
Sbjct: 116 KPRKVEEKTFKALNRRESGVRGKKAMKKIVRNVEKLDEDSDSEETQMDDLSEFEYLGRIE 175

Query: 153 LEGFEESGTRRRMPWEKDDDGIVLRRMKKKTV-TSAELNLDRVLLERLKGEASKMEKWVK 212
            +   +     +MPWE++++  +LRRMKK++V T+AEL LD  LL RL+ EASKM KWV 
Sbjct: 176 EKVESKDRFGGKMPWEREEERFILRRMKKESVPTTAELILDEGLLNRLRREASKMRKWVN 235

Query: 213 VNKVGVTQDVVNQIQFMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALV 272
           V K GVT+ VVN+I+ MW+ NELAM++FDVPL RNM+RA+EI+EMKTGG+VV SKK  LV
Sbjct: 236 VRKAGVTELVVNKIKSMWKLNELAMVRFDVPLCRNMERAQEIIEMKTGGLVVLSKKEFLV 295

Query: 273 IYRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEAS 332
           +YRG                                    Y S                 
Sbjct: 296 VYRG---------------------------------GPSYSS----------------- 355

Query: 333 SFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPF 392
                  E    +S SLYERE DRLLD LGPR++DWWM +P PVDAD+L EVV GYM P 
Sbjct: 356 -------EGQDEISSSLYEREADRLLDGLGPRYMDWWMRRPFPVDADLLPEVVNGYMTPS 415

Query: 393 RRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALK 452
           RRCPP T+  LTD  L +LR +A  LP HFVLGRN  LQGLA++I+KLWEK +IAKIA+K
Sbjct: 416 RRCPPNTRAKLTDEELTYLRNIAQPLPFHFVLGRNYGLQGLASAIVKLWEKCIIAKIAIK 426

Query: 453 WGVPNTDNEQMALELK 466
           WG  NT+NE+MA EL+
Sbjct: 476 WGALNTNNEEMADELR 426

[Close]

BLAST of Cucsat.G4812.T12 vs. ExPASy Swiss-Prot
Match: Q6YYA3 (Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0360100 PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 8.7e-71
Identity = 150/316 (47.47%), Postives = 203/316 (64.24%), Query Frame = 0


Query: 155 EESGTRRRMPW---EKDDDGIVLRRMKKKTV-TSAELNLDRVLLERLKGEASKMEKWVKV 214
           E+ G RR +PW     ++  +VLRR KK  V T AE  L+   LERL+  A   E+W + 
Sbjct: 117 EKKGIRRAVPWAAARDEETKVVLRREKKTRVPTRAETELEAGELERLRRAARGKERWARA 176

Query: 215 NKVGVTQDVVNQIQFMWER-NELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALV 274
            K G+T +VV +++  W +  ELA ++   PL R MDRAREI+E+KTGG+VVW++     
Sbjct: 177 KKAGITDEVVEEVRGQWAKGQELAGVRIVEPLRRCMDRAREILEIKTGGLVVWTRGGIHF 236

Query: 275 IYRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEAS 334
           +YRG +Y   L+++ + +  ++    +   T  + +  G Y S      NDND    EA 
Sbjct: 237 VYRGSSY---LENAKRHRDFVNYNEELSPVTSNNPTSQGKYWSKDETLTNDND----EAD 296

Query: 335 SFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPF 394
                  ++ +P+ G+LYERE +RLLD LGPRFIDWW + PLPVDAD+L EVVP +  PF
Sbjct: 297 D------KDDKPIKGTLYEREVNRLLDSLGPRFIDWWWNTPLPVDADLLPEVVPDFKTPF 356

Query: 395 RRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALK 454
           R+CPP  +  L D  L +LRK A  LPTHFVLGRN KLQGLAA+ILKLWEKS+IAK+A+K
Sbjct: 357 RQCPPGVRPALADEELTYLRKHARPLPTHFVLGRNTKLQGLAAAILKLWEKSLIAKVAVK 416

Query: 455 WGVPNTDNEQMALELK 466
            G+ NT++EQMA  LK
Sbjct: 417 VGIQNTNHEQMARNLK 419

[Close]

BLAST of Cucsat.G4812.T12 vs. ExPASy Swiss-Prot
Match: Q9FYT6 (Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Zea mays OX=4577 GN=CRS1 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.5e-65
Identity = 144/315 (45.71%), Postives = 186/315 (59.05%), Query Frame = 0


Query: 156 ESGTRRRMPW-EKDDDG--IVLRRMKK-KTVTSAELNLDRVLLERLKGEASKMEKWVKVN 215
           E    R +PW    D+G  + LRR KK +  T AE  L+   L RL+  A  + +W +  
Sbjct: 133 EKEKSRAVPWAAARDEGLKVALRREKKPREPTRAETELETHELRRLRRLARGIGRWARAK 192

Query: 216 KVGVTQDVVNQIQFMW-ERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALVI 275
           K GVT +VV +++  W    ELA ++   PL R+MDRAREI+E+KTGG+VVW+K +   +
Sbjct: 193 KAGVTDEVVKEVRREWASGEELAAVRIVEPLRRSMDRAREILEIKTGGLVVWTKGDMHFV 252

Query: 276 YRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASS 335
           YRG  Y  N KHS                    F  + H +     +     G+ +E   
Sbjct: 253 YRGSKYQQNAKHSHT------------------FLTNVHKDDAFQENDQSICGQKDE--- 312

Query: 336 FFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFR 395
                    +P+ G+LYERE +RLLD LGPRF+DWW   PLPVDAD+L E VPG   P+R
Sbjct: 313 ---------EPVKGTLYEREVNRLLDTLGPRFVDWWWDTPLPVDADLLPEFVPGSKTPYR 372

Query: 396 RCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKW 455
            CPP  +  L D  L +LRKLA  LPTHF LGRN +LQGLAA+ILKLWEKS+IAKIA+K 
Sbjct: 373 LCPPGVRPTLADEELTYLRKLARLLPTHFALGRNTRLQGLAAAILKLWEKSLIAKIAVKI 417

Query: 456 GVPNTDNEQMALELK 466
           G+ NT+NEQMA  LK
Sbjct: 433 GIQNTNNEQMAWNLK 417

[Close]

BLAST of Cucsat.G4812.T12 vs. ExPASy Swiss-Prot
Match: Q8L7C2 (CRM-domain containing factor CFM2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CFM2 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.5e-46
Identity = 116/317 (36.59%), Postives = 168/317 (53.00%), Query Frame = 0


Query: 165 WEKDDDGIVLRRMKKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQ 224
           W+K+ +  + R+ ++K  + AEL L    L RL+    ++ K +K+ K G+T+ +VN I 
Sbjct: 144 WKKETE--MERKKEEKVPSLAELTLPPAELRRLRTVGIRLTKKLKIGKAGITEGIVNGIH 203

Query: 225 FMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHST 284
             W   E+  +  +     NM R  +++E KTGG+V+W   + +++YRG NY      S 
Sbjct: 204 ERWRTTEVVKIFCEDISRMNMKRTHDVLETKTGGLVIWRSGSKILLYRGVNYQYPYFVSD 263

Query: 285 KKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQP--- 344
           +   H +      ++        G  +S   +SI +        SS   I ++ ++P   
Sbjct: 264 RDLAHEAASGASSMD-------QGVVDSREKQSIAE--------SSAPSITNKMVKPMLT 323

Query: 345 ------------LSGSL-YERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPP 404
                       L G +    E DRLL+ LGPRF DWW + PLPVD D+L  VVP Y  P
Sbjct: 324 QGVGSPDKVRFQLPGEVQLVEEADRLLEGLGPRFTDWWAYDPLPVDGDLLPAVVPDYRRP 383

Query: 405 FRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIAL 464
           FR  P      LTD  +  +R+L   LP HF LGRNR LQGLA +I+KLWEK  +AKIA+
Sbjct: 384 FRLLPYGVSPKLTDDEMTTIRRLGRPLPCHFALGRNRNLQGLAVAIVKLWEKCELAKIAV 443

Query: 465 KWGVPNTDNEQMALELK 466
           K GV NT++E MA ELK
Sbjct: 444 KRGVQNTNSELMAEELK 443

[Close]

BLAST of Cucsat.G4812.T12 vs. ExPASy Swiss-Prot
Match: Q2R1U8 (CRM-domain containing factor CFM3, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=CFM3 PE=3 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 4.4e-46
Identity = 121/325 (37.23%), Postives = 177/325 (54.46%), Query Frame = 0


Query: 147 GDFDLEGFEESGTRRRMPWE----KDDDGIVLRRMKKKTVTSAELNLDRVLLERLKGEAS 206
           G FD +  E +    R PWE      + G    R  +     AEL L    L RL+    
Sbjct: 134 GGFD-DDAESALVDARFPWELPMPPPEAG---PRAARSKAWMAELTLPEAELRRLRHAGM 193

Query: 207 KMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVW 266
           +++  +KV   GVT+++V +I+  W  +E+  +K     + NM    EI+E KTGG+V+W
Sbjct: 194 RLKSRIKVGGAGVTREIVERIRDRWRNDEVVRIKVTGTPALNMRLFHEILERKTGGLVIW 253

Query: 267 SKKNALVIYRGCNY--PLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSIND 326
               ++ +YRG  Y  P   K ++K    +  ++ +K E   H  L     +     + D
Sbjct: 254 RSGTSVSLYRGVAYDIPEPTKGTSKNTQTLGMKSSIK-EPPGHSLLPNEKVN----EMQD 313

Query: 327 NDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQE 386
           N+G     +     +   ++P+    YE E D+LLD+LGPR+ DW    P PVDAD+L  
Sbjct: 314 NNGALVSNAE----KDTLVEPVPEIKYEDEIDKLLDELGPRYDDWPRPDPSPVDADLLPA 373

Query: 387 VVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEK 446
            VPGY PPFR  P   + +L+     +LR+LA  LP HF LGR+R+LQGLAA+++KLWEK
Sbjct: 374 TVPGYKPPFRVLPYGVRPSLSRRDTTNLRRLARGLPPHFALGRSRQLQGLAAAMVKLWEK 433

Query: 447 SMIAKIALKWGVPNTDNEQMALELK 466
           S IAKIALK GV  T +E+MA ++K
Sbjct: 434 SSIAKIALKRGVQLTTSERMAEDIK 445

[Close]

BLAST of Cucsat.G4812.T12 vs. NCBI nr
Match: XP_011649926.1 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic [Cucumis sativus] >KGN63131.1 hypothetical protein Csa_021894 [Cucumis sativus])

HSP 1 Score: 927 bits (2397), Expect = 0.0
Identity = 462/465 (99.35%), Postives = 463/465 (99.57%), Query Frame = 0


Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL
Sbjct: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRMKLEEVEFG FDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGGFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP
Sbjct: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISPQNPVKVET 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLKHSTKKQVHISPQNPVKVET
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKHSTKKQVHISPQNPVKVET 300

Query: 301 DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP 360
           DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP
Sbjct: 301 DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP 360

Query: 361 RFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV 420
           RFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV
Sbjct: 361 RFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV 420

Query: 421 LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK 465
           LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK
Sbjct: 421 LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK 465

[Close]

BLAST of Cucsat.G4812.T12 vs. NCBI nr
Match: XP_008441260.1 (PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic [Cucumis melo])

HSP 1 Score: 828 bits (2138), Expect = 7.26e-296
Identity = 421/472 (89.19%), Postives = 435/472 (92.16%), Query Frame = 0


Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSS LP  LIPPSFRSHS LLHLSTH+PISAT TPSQSSVL EPPSISNAA+NL
Sbjct: 1   MLPTTSFSSPLPTILIPPSFRSHSSLLHLSTHHPISATFTPSQSSVLSEPPSISNAALNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQ QQ  EEGVDPANPKRRNGSD +G DKCSRALGD GI+KTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQTQQ--EEGVDPANPKRRNGSDRNGGDKCSRALGDRGIEKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRM LEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIV RRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMNLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVFRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           T+TSAELNLDR+LLERLK +ASKMEKWVKVNKVGVTQDVVN IQ  WERNELAMLKFDVP
Sbjct: 181 TMTSAELNLDRLLLERLKAKASKMEKWVKVNKVGVTQDVVNNIQLKWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHIS-------PQ 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLK STK QV  S        Q
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKPSTKMQVDSSCSKTQVLQQ 300

Query: 301 NPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDR 360
           NPVKVETDTH SLSGHYESGL++S ND+DGEWEEASSFFL+RH NLQPLSGSLYERETDR
Sbjct: 301 NPVKVETDTHSSLSGHYESGLDQSRNDDDGEWEEASSFFLMRHGNLQPLSGSLYERETDR 360

Query: 361 LLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420
           LLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH
Sbjct: 361 LLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420

Query: 421 SLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK 465
           SLPTHFVLGRNR LQGLA +ILKLWEKSMIAKIALKWGVPNT+NEQMA ELK
Sbjct: 421 SLPTHFVLGRNRGLQGLAGAILKLWEKSMIAKIALKWGVPNTNNEQMAFELK 470

[Close]

BLAST of Cucsat.G4812.T12 vs. NCBI nr
Match: TYK06679.1 (chloroplastic group IIA intron splicing facilitator CRS1 [Cucumis melo var. makuwa])

HSP 1 Score: 828 bits (2138), Expect = 4.81e-295
Identity = 421/472 (89.19%), Postives = 435/472 (92.16%), Query Frame = 0


Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSS LP  LIPPSFRSHS LLHLSTH+PISAT TPSQSSVL EPPSISNAA+NL
Sbjct: 1   MLPTTSFSSPLPTILIPPSFRSHSSLLHLSTHHPISATFTPSQSSVLSEPPSISNAALNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQ QQ  EEGVDPANPKRRNGSD +G DKCSRALGD GI+KTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQTQQ--EEGVDPANPKRRNGSDRNGGDKCSRALGDRGIEKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRM LEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIV RRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMNLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVFRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           T+TSAELNLDR+LLERLK +ASKMEKWVKVNKVGVTQDVVN IQ  WERNELAMLKFDVP
Sbjct: 181 TMTSAELNLDRLLLERLKAKASKMEKWVKVNKVGVTQDVVNNIQLKWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHIS-------PQ 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLK STK QV  S        Q
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKPSTKMQVDSSCSKTQVLQQ 300

Query: 301 NPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDR 360
           NPVKVETDTH SLSGHYESGL++S ND+DGEWEEASSFFL+RH NLQPLSGSLYERETDR
Sbjct: 301 NPVKVETDTHSSLSGHYESGLDQSRNDDDGEWEEASSFFLMRHGNLQPLSGSLYERETDR 360

Query: 361 LLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420
           LLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH
Sbjct: 361 LLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420

Query: 421 SLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK 465
           SLPTHFVLGRNR LQGLA +ILKLWEKSMIAKIALKWGVPNT+NEQMA ELK
Sbjct: 421 SLPTHFVLGRNRGLQGLAGAILKLWEKSMIAKIALKWGVPNTNNEQMAFELK 470

[Close]

BLAST of Cucsat.G4812.T12 vs. NCBI nr
Match: XP_038884368.1 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform X3 [Benincasa hispida])

HSP 1 Score: 755 bits (1950), Expect = 2.24e-267
Identity = 390/474 (82.28%), Postives = 417/474 (87.97%), Query Frame = 0


Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFS   PR LIPPSFRS S LLH S H PISATSTPSQSSVL EPPS SNAAVNL
Sbjct: 1   MLPTTSFS---PRILIPPSFRSDSTLLHFSIHKPISATSTPSQSSVLSEPPSTSNAAVNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMK PL+LQPQQ  EEGVD A P+RRNGSDG+G +KCSRAL D  IDKTGKYAMRR
Sbjct: 61  RTAPWMKGPLYLQPQQ--EEGVDLAKPERRNGSDGNGGEKCSRALADGRIDKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKL++NGD GETRMKLEEVEFG F LEGFEESGTRRRMPWEK D+GIVLRR KKK
Sbjct: 121 IAKSIGKLQKNGDSGETRMKLEEVEFGGFYLEGFEESGTRRRMPWEK-DNGIVLRRTKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           TVTSAELNLD++LLERL+  ASKMEKWVKVNKVGVT+DVVN+IQF+WERNELAMLKFDVP
Sbjct: 181 TVTSAELNLDKMLLERLRSAASKMEKWVKVNKVGVTEDVVNKIQFIWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQV--------HISP 300
           LSRNMDRAREIVEMKTGGMVVWSKKN LV+YRGCNYPL LKHST+ QV         +  
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNGLVVYRGCNYPLGLKHSTEMQVDSSYCSKTQVMQ 300

Query: 301 QNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETD 360
           QNPVK+ETD+ FS   HYE+ LN+SINDNDGEWEE S+ FL+   N QPLSGSLYERETD
Sbjct: 301 QNPVKLETDSDFS-GKHYENDLNQSINDNDGEWEETSTSFLMHQGNFQPLSGSLYERETD 360

Query: 361 RLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLA 420
           RLLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPP+TK NLTDA L HLRKLA
Sbjct: 361 RLLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPFTKPNLTDAELLHLRKLA 420

Query: 421 HSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKA 466
           HSLPTHFVLGRNRKLQGLAA+ILKLWEKS+IAKIALKWGVPNT+NEQMA ELK+
Sbjct: 421 HSLPTHFVLGRNRKLQGLAAAILKLWEKSLIAKIALKWGVPNTNNEQMACELKS 467

[Close]

BLAST of Cucsat.G4812.T12 vs. NCBI nr
Match: XP_038884367.1 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 755 bits (1950), Expect = 2.57e-267
Identity = 390/474 (82.28%), Postives = 417/474 (87.97%), Query Frame = 0


Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFS   PR LIPPSFRS S LLH S H PISATSTPSQSSVL EPPS SNAAVNL
Sbjct: 1   MLPTTSFS---PRILIPPSFRSDSTLLHFSIHKPISATSTPSQSSVLSEPPSTSNAAVNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMK PL+LQPQQ  EEGVD A P+RRNGSDG+G +KCSRAL D  IDKTGKYAMRR
Sbjct: 61  RTAPWMKGPLYLQPQQ--EEGVDLAKPERRNGSDGNGGEKCSRALADGRIDKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKL++NGD GETRMKLEEVEFG F LEGFEESGTRRRMPWEK D+GIVLRR KKK
Sbjct: 121 IAKSIGKLQKNGDSGETRMKLEEVEFGGFYLEGFEESGTRRRMPWEK-DNGIVLRRTKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           TVTSAELNLD++LLERL+  ASKMEKWVKVNKVGVT+DVVN+IQF+WERNELAMLKFDVP
Sbjct: 181 TVTSAELNLDKMLLERLRSAASKMEKWVKVNKVGVTEDVVNKIQFIWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQV--------HISP 300
           LSRNMDRAREIVEMKTGGMVVWSKKN LV+YRGCNYPL LKHST+ QV         +  
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNGLVVYRGCNYPLGLKHSTEMQVDSSYCSKTQVMQ 300

Query: 301 QNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETD 360
           QNPVK+ETD+ FS   HYE+ LN+SINDNDGEWEE S+ FL+   N QPLSGSLYERETD
Sbjct: 301 QNPVKLETDSDFS-GKHYENDLNQSINDNDGEWEETSTSFLMHQGNFQPLSGSLYERETD 360

Query: 361 RLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLA 420
           RLLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPP+TK NLTDA L HLRKLA
Sbjct: 361 RLLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPFTKPNLTDAELLHLRKLA 420

Query: 421 HSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKA 466
           HSLPTHFVLGRNRKLQGLAA+ILKLWEKS+IAKIALKWGVPNT+NEQMA ELK+
Sbjct: 421 HSLPTHFVLGRNRKLQGLAAAILKLWEKSLIAKIALKWGVPNTNNEQMACELKS 467

[Close]

BLAST of Cucsat.G4812.T12 vs. ExPASy TrEMBL
Match: A0A0A0LSY1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G404830 PE=4 SV=1)

HSP 1 Score: 927 bits (2397), Expect = 0.0
Identity = 462/465 (99.35%), Postives = 463/465 (99.57%), Query Frame = 0


Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL
Sbjct: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRMKLEEVEFG FDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGGFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP
Sbjct: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISPQNPVKVET 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLKHSTKKQVHISPQNPVKVET
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKHSTKKQVHISPQNPVKVET 300

Query: 301 DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP 360
           DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP
Sbjct: 301 DTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDDLGP 360

Query: 361 RFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV 420
           RFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV
Sbjct: 361 RFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFV 420

Query: 421 LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK 465
           LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK
Sbjct: 421 LGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK 465

[Close]

BLAST of Cucsat.G4812.T12 vs. ExPASy TrEMBL
Match: A0A1S3B2K3 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103485442 PE=4 SV=1)

HSP 1 Score: 828 bits (2138), Expect = 3.51e-296
Identity = 421/472 (89.19%), Postives = 435/472 (92.16%), Query Frame = 0


Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSS LP  LIPPSFRSHS LLHLSTH+PISAT TPSQSSVL EPPSISNAA+NL
Sbjct: 1   MLPTTSFSSPLPTILIPPSFRSHSSLLHLSTHHPISATFTPSQSSVLSEPPSISNAALNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQ QQ  EEGVDPANPKRRNGSD +G DKCSRALGD GI+KTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQTQQ--EEGVDPANPKRRNGSDRNGGDKCSRALGDRGIEKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRM LEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIV RRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMNLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVFRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           T+TSAELNLDR+LLERLK +ASKMEKWVKVNKVGVTQDVVN IQ  WERNELAMLKFDVP
Sbjct: 181 TMTSAELNLDRLLLERLKAKASKMEKWVKVNKVGVTQDVVNNIQLKWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHIS-------PQ 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLK STK QV  S        Q
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKPSTKMQVDSSCSKTQVLQQ 300

Query: 301 NPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDR 360
           NPVKVETDTH SLSGHYESGL++S ND+DGEWEEASSFFL+RH NLQPLSGSLYERETDR
Sbjct: 301 NPVKVETDTHSSLSGHYESGLDQSRNDDDGEWEEASSFFLMRHGNLQPLSGSLYERETDR 360

Query: 361 LLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420
           LLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH
Sbjct: 361 LLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420

Query: 421 SLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK 465
           SLPTHFVLGRNR LQGLA +ILKLWEKSMIAKIALKWGVPNT+NEQMA ELK
Sbjct: 421 SLPTHFVLGRNRGLQGLAGAILKLWEKSMIAKIALKWGVPNTNNEQMAFELK 470

[Close]

BLAST of Cucsat.G4812.T12 vs. ExPASy TrEMBL
Match: A0A5D3C8J4 (Chloroplastic group IIA intron splicing facilitator CRS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G001730 PE=4 SV=1)

HSP 1 Score: 828 bits (2138), Expect = 2.33e-295
Identity = 421/472 (89.19%), Postives = 435/472 (92.16%), Query Frame = 0


Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60
           MLPTTSFSS LP  LIPPSFRSHS LLHLSTH+PISAT TPSQSSVL EPPSISNAA+NL
Sbjct: 1   MLPTTSFSSPLPTILIPPSFRSHSSLLHLSTHHPISATFTPSQSSVLSEPPSISNAALNL 60

Query: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120
           RTAPWMKAPLHLQ QQ  EEGVDPANPKRRNGSD +G DKCSRALGD GI+KTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQTQQ--EEGVDPANPKRRNGSDRNGGDKCSRALGDRGIEKTGKYAMRR 120

Query: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180
           IAKSIGKLRRNGDLGETRM LEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIV RRMKKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMNLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVFRRMKKK 180

Query: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240
           T+TSAELNLDR+LLERLK +ASKMEKWVKVNKVGVTQDVVN IQ  WERNELAMLKFDVP
Sbjct: 181 TMTSAELNLDRLLLERLKAKASKMEKWVKVNKVGVTQDVVNNIQLKWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHIS-------PQ 300
           LSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPLNLK STK QV  S        Q
Sbjct: 241 LSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLNLKPSTKMQVDSSCSKTQVLQQ 300

Query: 301 NPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDR 360
           NPVKVETDTH SLSGHYESGL++S ND+DGEWEEASSFFL+RH NLQPLSGSLYERETDR
Sbjct: 301 NPVKVETDTHSSLSGHYESGLDQSRNDDDGEWEEASSFFLMRHGNLQPLSGSLYERETDR 360

Query: 361 LLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420
           LLDDLGPRFIDWWMHKPLPVDADML EVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH
Sbjct: 361 LLDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAH 420

Query: 421 SLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK 465
           SLPTHFVLGRNR LQGLA +ILKLWEKSMIAKIALKWGVPNT+NEQMA ELK
Sbjct: 421 SLPTHFVLGRNRGLQGLAGAILKLWEKSMIAKIALKWGVPNTNNEQMAFELK 470

[Close]

BLAST of Cucsat.G4812.T12 vs. ExPASy TrEMBL
Match: A0A6J1JY40 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489335 PE=4 SV=1)

HSP 1 Score: 662 bits (1709), Expect = 2.83e-231
Identity = 359/472 (76.06%), Postives = 387/472 (81.99%), Query Frame = 0


Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNP---ISATSTPSQSSVLPEPPSISNAA 60
           ML TT FS +LPR L PPSF S S  LHLS+H P   I ATST SQSS+L EP S SNAA
Sbjct: 1   MLATTCFSPALPRILFPPSFLSDSTPLHLSSHKPRAPIPATSTGSQSSLLSEPSSDSNAA 60

Query: 61  VNLRTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYA 120
           +N RTAPW ++PL+LQP   EEEGVD A P+R NGSDG G  K  RAL D    KTGKYA
Sbjct: 61  INHRTAPWTESPLYLQP--LEEEGVDLAKPERSNGSDGHGGKKRYRALADRRNVKTGKYA 120

Query: 121 MRRIAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRM 180
           MRRIAKSI KL++NGD GET MKLEEVEFGDF LEGF+ES TR RMPWEK DDGIVLRR 
Sbjct: 121 MRRIAKSIEKLQKNGDSGETGMKLEEVEFGDFYLEGFDESRTRGRMPWEK-DDGIVLRRP 180

Query: 181 KKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKF 240
           KKKTVTSAEL+LD+VLLERL+GEASKME WVKVNK+GVTQDVVN+I F WE NELAMLKF
Sbjct: 181 KKKTVTSAELSLDKVLLERLRGEASKMENWVKVNKIGVTQDVVNKIHFTWETNELAMLKF 240

Query: 241 DVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISP---QN 300
           DVPLSRNMDRAREIVEMKTGGMVVWSKKNALV+YRGCNYPL LK  TK QV  SP   + 
Sbjct: 241 DVPLSRNMDRAREIVEMKTGGMVVWSKKNALVVYRGCNYPLGLKLRTKMQVD-SPYCSRT 300

Query: 301 PVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRL 360
           P  +ETDT FSL  H E G NRSIN+NDGEW EAS+ FL         SGSLYERETDRL
Sbjct: 301 PF-LETDTDFSLGEHDEGGSNRSINNNDGEWGEASTSFL---------SGSLYERETDRL 360

Query: 361 LDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHS 420
           LDDLGPRFIDWWMHKPLPVDADML EVVPGYMPP+RRCPP+T   LTDA L +LRKLAH 
Sbjct: 361 LDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPYRRCPPFTNPKLTDAELLYLRKLAHP 420

Query: 421 LPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKA 466
           LPTHFVLGRNRKLQGLAA+ILKLWEKS+IAKIALKWGVPNT+NEQMA ELK+
Sbjct: 421 LPTHFVLGRNRKLQGLAAAILKLWEKSLIAKIALKWGVPNTNNEQMAYELKS 458

[Close]

BLAST of Cucsat.G4812.T12 vs. ExPASy TrEMBL
Match: A0A6J1FHM9 (chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445437 PE=4 SV=1)

HSP 1 Score: 656 bits (1692), Expect = 1.06e-228
Identity = 358/472 (75.85%), Postives = 385/472 (81.57%), Query Frame = 0


Query: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNP---ISATSTPSQSSVLPEPPSISNAA 60
           ML TT FS +LP  L PPSFRS S  LHLS+H P   I ATST SQSS+L  P S SNAA
Sbjct: 1   MLATTCFSPALPTILFPPSFRSDSTPLHLSSHKPRAPIPATSTGSQSSLLSGPSSDSNAA 60

Query: 61  VNLRTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYA 120
           +NLRTAPW KAPL+LQP  QEEEGVD ANP+R NGSDG G  K  RAL D    KTGKYA
Sbjct: 61  INLRTAPWTKAPLYLQP--QEEEGVDLANPERSNGSDGHGDKKRYRALADRRNVKTGKYA 120

Query: 121 MRRIAKSIGKLRRNGDLGETRMKLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVLRRM 180
           MRRIAKSI KL++NGD GET MKLEEVEFGDF LEGF+ES TR RMPWEK DDGIVLRR 
Sbjct: 121 MRRIAKSIEKLQKNGDSGETGMKLEEVEFGDFYLEGFDESRTRGRMPWEK-DDGIVLRRP 180

Query: 181 KKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKF 240
           KKKTVTSAEL+LD+VLLERL+GEASK+EKWVKVNK+GVTQDVVN+I F WE NELAMLKF
Sbjct: 181 KKKTVTSAELSLDKVLLERLRGEASKIEKWVKVNKIGVTQDVVNKIHFTWETNELAMLKF 240

Query: 241 DVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISP---QN 300
           DVPL RNMDRAREIVEMKTGGMVVWSKKNA V+YRGCNYPL LK STK QV  SP   + 
Sbjct: 241 DVPLCRNMDRAREIVEMKTGGMVVWSKKNAHVVYRGCNYPLGLKLSTKMQVD-SPYCSRT 300

Query: 301 PVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRL 360
           P  +ETDT FSL    E G NRSIN+N  EW EAS+ FL         SGSLYERETDRL
Sbjct: 301 PF-LETDTDFSLDEPDEGGSNRSINNNVDEWGEASTSFL---------SGSLYERETDRL 360

Query: 361 LDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHS 420
           LDDLGPRFIDWWMHKPLPVDADML EVVPGYMPP+RRCPP+T   LTDA L +LRKLAH 
Sbjct: 361 LDDLGPRFIDWWMHKPLPVDADMLPEVVPGYMPPYRRCPPFTNPKLTDAELLYLRKLAHP 420

Query: 421 LPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELKA 466
           LPTHFVLGRN KLQGLAA+ILKLWEKS+IAKIALKWGVPNT+NEQMA ELK+
Sbjct: 421 LPTHFVLGRNTKLQGLAAAILKLWEKSLIAKIALKWGVPNTNNEQMACELKS 458

[Close]

BLAST of Cucsat.G4812.T12 vs. TAIR 10
Match: AT5G16180.1 (ortholog of maize chloroplast splicing factor CRS1 )

HSP 1 Score: 324.7 bits (831), Expect = 1.2e-88
Identity = 198/436 (45.41%), Postives = 257/436 (58.94%), Query Frame = 0


Query: 33  NPISATSTPSQSSVLPEPPSISNAAVNLRTAPWMKAPLHLQPQQQEEEGVDPANPKRRNG 92
           N  S  +  SQ     E   +S+AA+ + TAPWMK PL L+P        +  + K+RN 
Sbjct: 56  NSSSRRAKSSQFDQFRENRGVSDAAIKVPTAPWMKGPLLLRPD-------EILDTKKRN- 115

Query: 93  SDGSGRDKCSRALGDSGIDKTGKYAMRRIAKSIGKLRRNGDLGETRM-KLEEVEF-GDFD 152
                 +K  +AL        GK AM++I +++ KL  + D  ET+M  L E E+ G  +
Sbjct: 116 KPRKVEEKTFKALNRRESGVRGKKAMKKIVRNVEKLDEDSDSEETQMDDLSEFEYLGRIE 175

Query: 153 LEGFEESGTRRRMPWEKDDDGIVLRRMKKKTV-TSAELNLDRVLLERLKGEASKMEKWVK 212
            +   +     +MPWE++++  +LRRMKK++V T+AEL LD  LL RL+ EASKM KWV 
Sbjct: 176 EKVESKDRFGGKMPWEREEERFILRRMKKESVPTTAELILDEGLLNRLRREASKMRKWVN 235

Query: 213 VNKVGVTQDVVNQIQFMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALV 272
           V K GVT+ VVN+I+ MW+ NELAM++FDVPL RNM+RA+EI+EMKTGG+VV SKK  LV
Sbjct: 236 VRKAGVTELVVNKIKSMWKLNELAMVRFDVPLCRNMERAQEIIEMKTGGLVVLSKKEFLV 295

Query: 273 IYRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEAS 332
           +YRG                                    Y S                 
Sbjct: 296 VYRG---------------------------------GPSYSS----------------- 355

Query: 333 SFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPF 392
                  E    +S SLYERE DRLLD LGPR++DWWM +P PVDAD+L EVV GYM P 
Sbjct: 356 -------EGQDEISSSLYEREADRLLDGLGPRYMDWWMRRPFPVDADLLPEVVNGYMTPS 415

Query: 393 RRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALK 452
           RRCPP T+  LTD  L +LR +A  LP HFVLGRN  LQGLA++I+KLWEK +IAKIA+K
Sbjct: 416 RRCPPNTRAKLTDEELTYLRNIAQPLPFHFVLGRNYGLQGLASAIVKLWEKCIIAKIAIK 426

Query: 453 WGVPNTDNEQMALELK 466
           WG  NT+NE+MA EL+
Sbjct: 476 WGALNTNNEEMADELR 426

[Close]

BLAST of Cucsat.G4812.T12 vs. TAIR 10
Match: AT3G18390.1 (CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 197.6 bits (501), Expect = 2.3e-50
Identity = 123/316 (38.92%), Postives = 179/316 (56.65%), Query Frame = 0


Query: 163 MPWEKDDD-----------GI-VLRRMKKKTVTSAELNLDRVLLERLKGEASKMEKWVKV 222
           +PWEK+++           G+ V+++ + +  + AEL ++   L RL+ +   +   + +
Sbjct: 205 LPWEKNEEEQAAERVVGEGGVAVMQKRRARAPSLAELTVEDSELRRLRRDGMYLRVRINI 264

Query: 223 NKVGVTQDVVNQIQFMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALVI 282
            K G+TQ V+ +I   W + EL  LKF   L+R+M  A EIVE +TGGMV+W   + +V+
Sbjct: 265 PKAGLTQAVMEKIYDTWRKEELVRLKFHEVLARDMKTAHEIVERRTGGMVIWRAGSVMVV 324

Query: 283 YRGCNYPLNLKHSTKKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDG-EWEEAS 342
           YRG +Y        K    IS Q     ET     +S   +   N   N +     ++  
Sbjct: 325 YRGLDY--------KGPPVISNQMAGPKETLFVPDVSSAGDEATNAKDNQSAPLVIKDPI 384

Query: 343 SFFLIRHENLQPLSGSLYERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPF 402
               IR EN+     +  E E + LLD LGPRF +WW    LPVDAD+L   +PGY  PF
Sbjct: 385 IKNPIRKENM-----TEEEVEFNSLLDSLGPRFQEWWGTGVLPVDADLLPPTIPGYKTPF 444

Query: 403 RRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIALK 462
           R  P   + NLT+A + +LRK+  +LP HF LGRNR  QGLAA+IL++WEKS+IAKIA+K
Sbjct: 445 RLLPTGMRSNLTNAEMTNLRKIGKTLPCHFALGRNRNHQGLAAAILQIWEKSLIAKIAVK 504

Query: 463 WGVPNTDNEQMALELK 466
            G+ NT+N+ MA E+K
Sbjct: 505 RGIQNTNNKLMADEVK 507

[Close]

BLAST of Cucsat.G4812.T12 vs. TAIR 10
Match: AT4G29750.1 (CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 191.0 bits (484), Expect = 2.1e-48
Identity = 116/290 (40.00%), Postives = 160/290 (55.17%), Query Frame = 0


Query: 176 RMKKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAML 235
           R +K     AE  +    L+RL+  A +M + VKV   G+TQ +V  I   WE +E+  L
Sbjct: 231 RTRKSNTVEAERIVPEHELKRLRNVALRMVERVKVGSAGITQALVEAIHEKWEVDEVVKL 290

Query: 236 KFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISPQNP 295
           KF  P S NM R  E++E KTGG+V+W   +++V+YRG +Y L    +  K      QN 
Sbjct: 291 KFSEPYSLNMKRTHEVLEKKTGGLVIWRSGSSVVLYRGISYKLKCVQTFIK------QNN 350

Query: 296 VKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLL 355
           ++   + H            RS+   D   E+ +    +  E L  L       E + LL
Sbjct: 351 LEANPEIH------------RSVEARDYVQEDGNYPKNVPKEQLSELC------ELNDLL 410

Query: 356 DDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSL 415
           D++GPRF DW    P PVDAD+L   V GY  PFR  P   K  L++  +  +R+LA + 
Sbjct: 411 DEVGPRFHDWTGCAPFPVDADLLPGYVEGYRCPFRILPQGVKPCLSNTEMTEMRRLARTS 470

Query: 416 PTHFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK 466
           P HF LGR+R+LQGLA +++KLW KS IAKIA+K GV NT NE+MA ELK
Sbjct: 471 PPHFALGRSRELQGLAKAMVKLWAKSAIAKIAIKRGVENTRNERMAEELK 496

[Close]

BLAST of Cucsat.G4812.T12 vs. TAIR 10
Match: AT3G01370.1 (CRM family member 2 )

HSP 1 Score: 188.7 bits (478), Expect = 1.1e-47
Identity = 116/317 (36.59%), Postives = 168/317 (53.00%), Query Frame = 0


Query: 165 WEKDDDGIVLRRMKKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQ 224
           W+K+ +  + R+ ++K  + AEL L    L RL+    ++ K +K+ K G+T+ +VN I 
Sbjct: 144 WKKETE--MERKKEEKVPSLAELTLPPAELRRLRTVGIRLTKKLKIGKAGITEGIVNGIH 203

Query: 225 FMWERNELAMLKFDVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHST 284
             W   E+  +  +     NM R  +++E KTGG+V+W   + +++YRG NY      S 
Sbjct: 204 ERWRTTEVVKIFCEDISRMNMKRTHDVLETKTGGLVIWRSGSKILLYRGVNYQYPYFVSD 263

Query: 285 KKQVHISPQNPVKVETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQP--- 344
           +   H +      ++        G  +S   +SI +        SS   I ++ ++P   
Sbjct: 264 RDLAHEAASGASSMD-------QGVVDSREKQSIAE--------SSAPSITNKMVKPMLT 323

Query: 345 ------------LSGSL-YERETDRLLDDLGPRFIDWWMHKPLPVDADMLQEVVPGYMPP 404
                       L G +    E DRLL+ LGPRF DWW + PLPVD D+L  VVP Y  P
Sbjct: 324 QGVGSPDKVRFQLPGEVQLVEEADRLLEGLGPRFTDWWAYDPLPVDGDLLPAVVPDYRRP 383

Query: 405 FRRCPPYTKQNLTDAGLQHLRKLAHSLPTHFVLGRNRKLQGLAASILKLWEKSMIAKIAL 464
           FR  P      LTD  +  +R+L   LP HF LGRNR LQGLA +I+KLWEK  +AKIA+
Sbjct: 384 FRLLPYGVSPKLTDDEMTTIRRLGRPLPCHFALGRNRNLQGLAVAIVKLWEKCELAKIAV 443

Query: 465 KWGVPNTDNEQMALELK 466
           K GV NT++E MA ELK
Sbjct: 444 KRGVQNTNSELMAEELK 443

[Close]

BLAST of Cucsat.G4812.T12 vs. TAIR 10
Match: AT3G23070.1 (CRM family member 3A )

HSP 1 Score: 181.4 bits (459), Expect = 1.7e-45
Identity = 107/288 (37.15%), Postives = 156/288 (54.17%), Query Frame = 0


Query: 178 KKKTVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKF 237
           K+   + AE+ L    L RL+    +    +++   GVTQ  V+ I+  W+  E+  LK 
Sbjct: 210 KENRYSLAEMTLPESELRRLRNLTFRTASKMRIRGGGVTQVAVDAIKEKWKSAEIVRLKI 269

Query: 238 DVPLSRNMDRAREIVEMKTGGMVVWSKKNALVIYRGCNYPLNLKHSTKKQVHISPQNPVK 297
           +   + NM +  EI+E KTGG+V+W    ++ +YRG +Y L      K++         +
Sbjct: 270 EGASALNMRKMHEILEKKTGGLVIWRSGTSISLYRGVSYELPSGKWNKQR---------R 329

Query: 298 VETDTHFSLSGHYESGLNRSINDNDGEWEEASSFFLIRHENLQPLSGSLYERETDRLLDD 357
            ET     +  H E+      +D      +         +  Q      YE E D LLDD
Sbjct: 330 EETPPEAVIENHDETTTMVDKSDEKVHLPQLEQETTSVEKKDQTSPVVEYEDELDELLDD 389

Query: 358 LGPRFIDWWMHKPLPVDADMLQEVVPGYMPPFRRCPPYTKQNLTDAGLQHLRKLAHSLPT 417
           LGPRF+DW    PLPVDAD+L   +P Y PPFR  P   + +L       LR+LA S+P 
Sbjct: 390 LGPRFMDWPGDNPLPVDADLLPGAIPDYEPPFRVLPYGVRSSLGPKEATALRRLARSIPP 449

Query: 418 HFVLGRNRKLQGLAASILKLWEKSMIAKIALKWGVPNTDNEQMALELK 466
           HF LGR+R+LQGLA ++++LWEKSM+AKIA+K GV +T +E+MA +LK
Sbjct: 450 HFALGRSRQLQGLATAMVRLWEKSMLAKIAIKRGVQSTTSERMAEDLK 488

The following BLAST results are available for this feature:

BLAST of Cucsat.G4812.T12 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LF10	1.8e-87	45.41	Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Arabi...	[more]
Q6YYA3	8.7e-71	47.47	Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Oryza...	[more]
Q9FYT6	2.5e-65	45.71	Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Zea m...	[more]
Q8L7C2	1.5e-46	36.59	CRM-domain containing factor CFM2, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q2R1U8	4.4e-46	37.23	CRM-domain containing factor CFM3, chloroplastic/mitochondrial OS=Oryza sativa s...	[more]

BLAST of Cucsat.G4812.T12 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011649926.1	0.0	99.35	chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic [Cucumis...	[more]
XP_008441260.1	7.26e-296	89.19	PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplast...	[more]
TYK06679.1	4.81e-295	89.19	chloroplastic group IIA intron splicing facilitator CRS1 [Cucumis melo var. maku...	[more]
XP_038884368.1	2.24e-267	82.28	chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform ...	[more]
XP_038884367.1	2.57e-267	82.28	chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform ...	[more]

BLAST of Cucsat.G4812.T12 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LSY1	0.0	99.35	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G404830 PE=4 SV=1	[more]
A0A1S3B2K3	3.51e-296	89.19	chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Cucum...	[more]
A0A5D3C8J4	2.33e-295	89.19	Chloroplastic group IIA intron splicing facilitator CRS1 OS=Cucumis melo var. ma...	[more]
A0A6J1JY40	2.83e-231	76.06	chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform ...	[more]
A0A6J1FHM9	1.06e-228	75.85	chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Cucur...	[more]

BLAST of Cucsat.G4812.T12 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16180.1	1.2e-88	45.41	ortholog of maize chloroplast splicing factor CRS1	[more]
AT3G18390.1	2.3e-50	38.92	CRS1 / YhbY (CRM) domain-containing protein	[more]
AT4G29750.1	2.1e-48	40.00	CRS1 / YhbY (CRM) domain-containing protein	[more]
AT3G01370.1	1.1e-47	36.59	CRM family member 2	[more]
AT3G23070.1	1.7e-45	37.15	CRM family member 3A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001890	RNA-binding, CRM domain	SMART	SM01103	CRS1_YhbY_2	coord: 189..272 e-value: 7.8E-9 score: 45.4 coord: 400..479 e-value: 6.5E-4 score: 27.0
IPR001890	RNA-binding, CRM domain	PFAM	PF01985	CRS1_YhbY	coord: 194..272 e-value: 1.9E-18 score: 66.4 coord: 400..466 e-value: 5.7E-6 score: 26.4
IPR001890	RNA-binding, CRM domain	PROSITE	PS51295	CRM	coord: 398..480 score: 11.779385
IPR001890	RNA-binding, CRM domain	PROSITE	PS51295	CRM	coord: 187..283 score: 15.799444
IPR035920	YhbY-like superfamily	GENE3D	3.30.110.60		coord: 188..290 e-value: 4.1E-14 score: 54.3 coord: 398..476 e-value: 1.5E-6 score: 30.0
IPR035920	YhbY-like superfamily	SUPERFAMILY	75471	YhbY-like	coord: 399..466
IPR035920	YhbY-like superfamily	SUPERFAMILY	75471	YhbY-like	coord: 193..277
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 82..106
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 74..109
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..53
None	No IPR available	PANTHER	PTHR31846:SF10	CHLOROPLASTIC GROUP IIA INTRON SPLICING FACILITATOR CRS1, CHLOROPLASTIC	coord: 28..466
None	No IPR available	PANTHER	PTHR31846	CRS1 / YHBY (CRM) DOMAIN-CONTAINING PROTEIN	coord: 28..466

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G4812	Cucsat.G4812	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G4812.T12.E1	Cucsat.G4812.T12.E1	exon
Cucsat.G4812.T12.E2	Cucsat.G4812.T12.E2	exon
Cucsat.G4812.T12.E3	Cucsat.G4812.T12.E3	exon
Cucsat.G4812.T12.E4	Cucsat.G4812.T12.E4	exon
Cucsat.G4812.T12.E5	Cucsat.G4812.T12.E5	exon
Cucsat.G4812.T12.E6	Cucsat.G4812.T12.E6	exon
Cucsat.G4812.T12.E7	Cucsat.G4812.T12.E7	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G4812.T12.C1	Cucsat.G4812.T12.C1	CDS
Cucsat.G4812.T12.C2	Cucsat.G4812.T12.C2	CDS
Cucsat.G4812.T12.C3	Cucsat.G4812.T12.C3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G4812.T12	Cucsat.G4812.T12-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000373	Group II intron splicing
molecular_function	GO:0003723	RNA binding