Cucsat.G2052.T7 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G2052.T7
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	GDSL esterase/lipase
Location	ctg1002: 6054222 .. 6056295 (-)
RNA-Seq Expression	Cucsat.G2052.T7
Synteny	Cucsat.G2052.T7

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTCTTCGTTTTCTCAACTGTAAAAGGCCAATTCCCTTTGATAGTTTATTCAATATCTTTTTCTATTTATACTCTATTTTCCACTTCAAAAATCTTCACAGATTAACAAAACTATCCATCCAACTTTATCACTGAATCTTCCAACTTTGTCTTTGAATTATGGGTATGGCTGCCAGTGGGGTGCTTCAAACTAATCAACGTCCATGCTCTTTCTTTCTTGCTTTGCTTCTTATCACTACTGCTTTCTCTTCTTCCAATGCTCAGCAACAATCCCAAGTTCCTTGCTATTTCATTTTTGGTGACTCTTTAGTTGACAATGGTAACAACAATCGTCTTTCTTCTTTGGCTCGTGCCGACTACCTCCCTTACGGTATCGACTTCCCTCGTGGCCCAACTGGAAGATTCTCCAATGGCAAAACCACCGTTGACGTCATTGGTAAATGCAAACCTGTTTCACTTTTTCATTTACTATGTTCATGATCATCACACTTAAACTGCAACCATCTTTGACTATAATATATAACCCATTCACCACATCAAACCATTCTAATTTTCACTAAAAAGAATCGATAATGTGTGTATATAATTAATTTTTTTTGTCTTATAGCGGAGTTGTTGGGGTTTAATGGCTACATCCCTCCCTATTCCAACACGAGAGGCCGAGACATACTTCGAGGTGTGAACTACGCGTCTGCAGCAGCAGGAATCAGAGAGGAAACTGGCCAACAATTGGTAATTAATTTATTTATGCTAAAGAATTTGAATTCAGAACATCATCACTCGTAAAGTTTTAATATTATATTATCTTTAACTTATATACATGTGTGCTGCAGGGAGGGCGAATTAGCTTTAGTGGGCAAGTAAGAAATCACCAAAACATAGTTACACAAATCGTAAATATTCTTGGTGATGAAAATACAGCTGCTGATTACTTGAACAAGTGTATATATTCAATTGGATTGGGTAGCAACGACTACCTTAACAACTATTTCATGCCTCAAATCTATTCGTCAAGTCGTCAATACGCGCCTGATCAATACGCTCAAATTCTCATTCAACAATACACACAACAGTTATCGGTCAGTACTTAACTGCTTAAGTATACGTGAGACTTTGATATATCACATTATTCATTAATTATAATAATTTTAAAAAATAAAAGCAAACATTTTTTTTTCTTCTGTTTTGTAGATATTGTATGACAATGGAGCAAGGAAGTTTGTGTTGTTTGGAGTGGGCCAAATTGGGTGCAGCCCAAATGCATTGGCAAGTAGCCCAGATGGGAGAAGTTGTAATCAAAGATACAACTTTGCAAATCAATTGTTCAACAACAGATTGAAAGGCCTTGTGGATCAACTCAACAGAAATCAGCCTGATGCTAGATTCATTTACATCGACTCTTATGGCATTTTCCAAGATATCATTAACAGCCCTTCATCTTTTGGTATAAATTATTATATTAATACACTCACTGATCTTCTTTTCCAATTTAATTAAATTTTAGTACTGTTATGAAGATTCAATAATTAATATTGTTGGGTGTGGTGCAGGTTTTAGAGTTACAAATGCTGGATGCTGTGGGATTGGGAGGAACAATGGCCAAATAACATGTTTGCCTTTTCAAACTCCTTGTGCCAACAGAAGAGAATATCTATTTTGGGATGCCTTCCATCCAACTGAGGCTGGAAACTCCATTGTTGGAAGAAGAGCTTACAGCGCTCAACGTTCCTCCGATGCCTACCCAATCGACATTCGTCGCCTTGCTCAACTCTAGGCTTAAATCCAACTTATAGGAAGTTATGTATAAGTTGTTGGAAGAGCTTTAATTAATTTCCCTTTAATTGCTATATGTGCCATTTCTTGAGAAAAGAACACGTGGGTTTGTGTGCTACTCAATTGATGGCTTGTGTTTGTATTTTAGGTGTTTGAGATTTTATCTATATCTTTCATGATATGTCCTAAGGAAAATCAATTATTGAGAATGAATATGTTATAAATATTATCTTACCAAAAGAATTGTGTTGTGACTTTTTATATCTATATACATATACACACATTACATGGTTGGAGACCA

Coding sequence (CDS)

ATGGGTATGGCTGCCAGTGGGGTGCTTCAAACTAATCAACGTCCATGCTCTTTCTTTCTTGCTTTGCTTCTTATCACTACTGCTTTCTCTTCTTCCAATGCTCAGCAACAATCCCAAGTTCCTTGCTATTTCATTTTTGGTGACTCTTTAGTTGACAATGGTAACAACAATCGTCTTTCTTCTTTGGCTCGTGCCGACTACCTCCCTTACGGTATCGACTTCCCTCGTGGCCCAACTGGAAGATTCTCCAATGGCAAAACCACCGTTGACGTCATTGCGGAGTTGTTGGGGTTTAATGGCTACATCCCTCCCTATTCCAACACGAGAGGCCGAGACATACTTCGAGGTGTGAACTACGCGTCTGCAGCAGCAGGAATCAGAGAGGAAACTGGCCAACAATTGGTAATTAATTTATTTATGCTAAAGAATTTGAATTCAGAACATCATCACTCGTAA

Protein sequence

MGMAASGVLQTNQRPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQLVINLFMLKNLNSEHHHS

Homology

[Close]

BLAST of Cucsat.G2052.T7 vs. ExPASy Swiss-Prot
Match: Q9C7N4 (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.2e-43
Identity = 85/115 (73.91%), Postives = 96/115 (83.48%), Query Frame = 0


Query: 20  LALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGIDFPRGPT 79
           + L+L+   FS   AQ Q+QVPC+F+FGDSLVDNGNNN L S+AR++Y PYGIDF  GPT
Sbjct: 10  VVLVLLCFGFSVVKAQAQAQVPCFFVFGDSLVDNGNNNGLISIARSNYFPYGIDF-GGPT 69

Query: 80  GRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQL 135
           GRFSNGKTTVDVIAELLGFNGYIP Y+   GR IL GVNYASAAAGIREETG+QL
Sbjct: 70  GRFSNGKTTVDVIAELLGFNGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQL 123

[Close]

BLAST of Cucsat.G2052.T7 vs. ExPASy Swiss-Prot
Match: Q93YW8 (GDSL esterase/lipase At4g18970 OS=Arabidopsis thaliana OX=3702 GN=At4g18970 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.1e-42
Identity = 85/121 (70.25%), Postives = 95/121 (78.51%), Query Frame = 0


Query: 14  RPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGID 73
           R C   +A+ +   A + + A      PCYFIFGDSLVD+GNNNRL+SLARA+Y PYGID
Sbjct: 3   RVCVMMMAMAI---AMAMNIAMGDPIAPCYFIFGDSLVDSGNNNRLTSLARANYFPYGID 62

Query: 74  FPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQ 133
           F  GPTGRFSNGKTTVDVI ELLGF+ YI PYS  RG DILRGVNYASAAAGIREETG+Q
Sbjct: 63  FQYGPTGRFSNGKTTVDVITELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQ 120

Query: 134 L 135
           L
Sbjct: 123 L 120

[Close]

BLAST of Cucsat.G2052.T7 vs. ExPASy Swiss-Prot
Match: Q9FK75 (GDSL esterase/lipase At5g45670 OS=Arabidopsis thaliana OX=3702 GN=At5g45670 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-41
Identity = 82/116 (70.69%), Postives = 95/116 (81.90%), Query Frame = 0


Query: 20  LALLLITTAFSSSN-AQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGIDFPRGP 79
           L +++I  A +  N A+     PCYFIFGDSLVDNGNNN+L SLARA+Y PYGIDF  GP
Sbjct: 6   LMIMMIMVAVTMINIAKSDPIAPCYFIFGDSLVDNGNNNQLQSLARANYFPYGIDFAAGP 65

Query: 80  TGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQL 135
           TGRFSNG TTVDVIA+LLGF  YI PY++ RG+DILRGVNYASAAAGIR+ETG+QL
Sbjct: 66  TGRFSNGLTTVDVIAQLLGFEDYITPYASARGQDILRGVNYASAAAGIRDETGRQL 121

[Close]

BLAST of Cucsat.G2052.T7 vs. ExPASy Swiss-Prot
Match: Q9C7N5 (GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 2.5e-40
Identity = 82/119 (68.91%), Postives = 94/119 (78.99%), Query Frame = 0


Query: 16  CSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGIDFP 75
           C   + +LL+   F     + + QVPCYFIFGDSLVDNGNNNRL S+ARADY PYGIDF 
Sbjct: 9   CLVSVWVLLLGLGF---KVKAEPQVPCYFIFGDSLVDNGNNNRLRSIARADYFPYGIDF- 68

Query: 76  RGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQL 135
            GPTGRFSNG+TTVDV+ ELLGF+ YIP YS   G++IL+GVNYASAAAGIREETG QL
Sbjct: 69  GGPTGRFSNGRTTVDVLTELLGFDNYIPAYSTVSGQEILQGVNYASAAAGIREETGAQL 123

[Close]

BLAST of Cucsat.G2052.T7 vs. ExPASy Swiss-Prot
Match: Q8L5Z1 (GDSL esterase/lipase At1g33811 OS=Arabidopsis thaliana OX=3702 GN=At1g33811 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 4.3e-40
Identity = 78/115 (67.83%), Postives = 91/115 (79.13%), Query Frame = 0


Query: 20  LALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGIDFPRGPT 79
           L L+L     + S  QQQ+QVPC FIFGDSLVDNGNNNRL SLARA+Y PYGIDFP+G T
Sbjct: 12  LNLVLFGFKTTVSQPQQQAQVPCLFIFGDSLVDNGNNNRLLSLARANYRPYGIDFPQGTT 71

Query: 80  GRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQL 135
           GRF+NG+T VD +A++LGF  YIPPYS  RG+ ILRG N+AS AAGIR+ETG  L
Sbjct: 72  GRFTNGRTYVDALAQILGFRNYIPPYSRIRGQAILRGANFASGAAGIRDETGDNL 126

[Close]

BLAST of Cucsat.G2052.T7 vs. NCBI nr
Match: XP_004134596.1 (GDSL esterase/lipase At1g29670 [Cucumis sativus] >KGN49344.1 hypothetical protein Csa_003759 [Cucumis sativus])

HSP 1 Score: 273 bits (698), Expect = 9.98e-89
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MGMAASGVLQTNQRPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLS 60
           MGMAASGVLQTNQRPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLS
Sbjct: 1   MGMAASGVLQTNQRPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLS 60

Query: 61  SLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYA 120
           SLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYA
Sbjct: 61  SLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYA 120

Query: 121 SAAAGIREETGQQL 134
           SAAAGIREETGQQL
Sbjct: 121 SAAAGIREETGQQL 134

[Close]

BLAST of Cucsat.G2052.T7 vs. NCBI nr
Match: XP_008439698.1 (PREDICTED: GDSL esterase/lipase At1g29670-like [Cucumis melo])

HSP 1 Score: 249 bits (637), Expect = 1.74e-79
Identity = 125/135 (92.59%), Postives = 126/135 (93.33%), Query Frame = 0


Query: 1   MGMAASGVLQTNQRP-CSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRL 60
           MGMAA GVLQTNQR  C  FLALLL+T   SSSNAQQQ QVPCYFIFGDSLVDNGNNNRL
Sbjct: 1   MGMAAGGVLQTNQRRLCPLFLALLLVTAFSSSSNAQQQPQVPCYFIFGDSLVDNGNNNRL 60

Query: 61  SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY 120
           SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY
Sbjct: 61  SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY 120

Query: 121 ASAAAGIREETGQQL 134
           ASAAAGIREETGQQL
Sbjct: 121 ASAAAGIREETGQQL 135

[Close]

BLAST of Cucsat.G2052.T7 vs. NCBI nr
Match: KAA0052587.1 (GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK13238.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 249 bits (637), Expect = 1.74e-79
Identity = 125/135 (92.59%), Postives = 126/135 (93.33%), Query Frame = 0


Query: 1   MGMAASGVLQTNQRP-CSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRL 60
           MGMAA GVLQTNQR  C  FLALLL+T   SSSNAQQQ QVPCYFIFGDSLVDNGNNNRL
Sbjct: 1   MGMAAGGVLQTNQRRLCPLFLALLLVTAFSSSSNAQQQPQVPCYFIFGDSLVDNGNNNRL 60

Query: 61  SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY 120
           SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY
Sbjct: 61  SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY 120

Query: 121 ASAAAGIREETGQQL 134
           ASAAAGIREETGQQL
Sbjct: 121 ASAAAGIREETGQQL 135

[Close]

BLAST of Cucsat.G2052.T7 vs. NCBI nr
Match: XP_038881173.1 (GDSL esterase/lipase At1g29670-like [Benincasa hispida])

HSP 1 Score: 228 bits (580), Expect = 6.42e-71
Identity = 115/134 (85.82%), Postives = 118/134 (88.06%), Query Frame = 0


Query: 1   MGMAASGVLQTNQRPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLS 60
           MGMA  G +Q NQ      L  LL+ T F SSNAQQQ QVPCYFIFGDSLVDNGNNNRLS
Sbjct: 1   MGMA--GGVQNNQHLWGTLLLPLLLITVFCSSNAQQQPQVPCYFIFGDSLVDNGNNNRLS 60

Query: 61  SLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYA 120
           SLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDIL+GVNYA
Sbjct: 61  SLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILQGVNYA 120

Query: 121 SAAAGIREETGQQL 134
           SAAAGIREETGQQL
Sbjct: 121 SAAAGIREETGQQL 132

[Close]

BLAST of Cucsat.G2052.T7 vs. NCBI nr
Match: KAG6581389.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034674.1 GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 206 bits (523), Expect = 2.15e-62
Identity = 105/132 (79.55%), Postives = 109/132 (82.58%), Query Frame = 0


Query: 3   MAASGVLQTNQRPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSL 62
           MA  G +  NQ        LL+   AF    AQQQ QVPCYFIFGDSLVDNGNNNRLSSL
Sbjct: 1   MAMGGKVVRNQ-----LWGLLVAVLAFWCCTAQQQPQVPCYFIFGDSLVDNGNNNRLSSL 60

Query: 63  ARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASA 122
           ARA+YLPYGIDFP+G TGRFSNGKTTVDVIAELLGFN YIPPYSNTRGRDILRGVNYASA
Sbjct: 61  ARANYLPYGIDFPQGTTGRFSNGKTTVDVIAELLGFNAYIPPYSNTRGRDILRGVNYASA 120

Query: 123 AAGIREETGQQL 134
           AAGIREETGQQL
Sbjct: 121 AAGIREETGQQL 127

[Close]

BLAST of Cucsat.G2052.T7 vs. ExPASy TrEMBL
Match: A0A0A0KKH4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520420 PE=3 SV=1)

HSP 1 Score: 273 bits (698), Expect = 4.83e-89
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MGMAASGVLQTNQRPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLS 60
           MGMAASGVLQTNQRPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLS
Sbjct: 1   MGMAASGVLQTNQRPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLS 60

Query: 61  SLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYA 120
           SLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYA
Sbjct: 61  SLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYA 120

Query: 121 SAAAGIREETGQQL 134
           SAAAGIREETGQQL
Sbjct: 121 SAAAGIREETGQQL 134

[Close]

BLAST of Cucsat.G2052.T7 vs. ExPASy TrEMBL
Match: A0A5D3CPY8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008530 PE=3 SV=1)

HSP 1 Score: 249 bits (637), Expect = 8.40e-80
Identity = 125/135 (92.59%), Postives = 126/135 (93.33%), Query Frame = 0


Query: 1   MGMAASGVLQTNQRP-CSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRL 60
           MGMAA GVLQTNQR  C  FLALLL+T   SSSNAQQQ QVPCYFIFGDSLVDNGNNNRL
Sbjct: 1   MGMAAGGVLQTNQRRLCPLFLALLLVTAFSSSSNAQQQPQVPCYFIFGDSLVDNGNNNRL 60

Query: 61  SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY 120
           SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY
Sbjct: 61  SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY 120

Query: 121 ASAAAGIREETGQQL 134
           ASAAAGIREETGQQL
Sbjct: 121 ASAAAGIREETGQQL 135

[Close]

BLAST of Cucsat.G2052.T7 vs. ExPASy TrEMBL
Match: A0A1S3AYY6 (GDSL esterase/lipase At1g29670-like OS=Cucumis melo OX=3656 GN=LOC103484416 PE=3 SV=1)

HSP 1 Score: 249 bits (637), Expect = 8.40e-80
Identity = 125/135 (92.59%), Postives = 126/135 (93.33%), Query Frame = 0


Query: 1   MGMAASGVLQTNQRP-CSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRL 60
           MGMAA GVLQTNQR  C  FLALLL+T   SSSNAQQQ QVPCYFIFGDSLVDNGNNNRL
Sbjct: 1   MGMAAGGVLQTNQRRLCPLFLALLLVTAFSSSSNAQQQPQVPCYFIFGDSLVDNGNNNRL 60

Query: 61  SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY 120
           SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY
Sbjct: 61  SSLARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNY 120

Query: 121 ASAAAGIREETGQQL 134
           ASAAAGIREETGQQL
Sbjct: 121 ASAAAGIREETGQQL 135

[Close]

BLAST of Cucsat.G2052.T7 vs. ExPASy TrEMBL
Match: A0A6J1INT6 (GDSL esterase/lipase At1g29670-like OS=Cucurbita maxima OX=3661 GN=LOC111478592 PE=3 SV=1)

HSP 1 Score: 205 bits (521), Expect = 2.09e-62
Identity = 105/132 (79.55%), Postives = 109/132 (82.58%), Query Frame = 0


Query: 3   MAASGVLQTNQRPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSL 62
           MA  G +  NQ        LL+   AF    AQQQ QVPCYFIFGDSLVDNGNNNRLSSL
Sbjct: 1   MAMGGKVVRNQ-----LWGLLVAVLAFWCCTAQQQPQVPCYFIFGDSLVDNGNNNRLSSL 60

Query: 63  ARADYLPYGIDFPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASA 122
           ARA+YLPYGIDFP+G TGRFSNGKTTVDVIAELLGFN YIPPYSNTRGRDILRGVNYASA
Sbjct: 61  ARANYLPYGIDFPQGTTGRFSNGKTTVDVIAELLGFNTYIPPYSNTRGRDILRGVNYASA 120

Query: 123 AAGIREETGQQL 134
           AAGIREETGQQL
Sbjct: 121 AAGIREETGQQL 127

[Close]

BLAST of Cucsat.G2052.T7 vs. ExPASy TrEMBL
Match: A0A6J1ECX6 (GDSL esterase/lipase At1g29670-like OS=Cucurbita moschata OX=3662 GN=LOC111433039 PE=3 SV=1)

HSP 1 Score: 203 bits (516), Expect = 1.12e-61
Identity = 99/113 (87.61%), Postives = 103/113 (91.15%), Query Frame = 0


Query: 22  LLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGIDFPRGPTGR 81
           +L+   AF    AQQQ QVPCYFIFGDSLVDNGNNNRLSSLARA+YLPYGIDFP+G TGR
Sbjct: 13  MLVAVLAFWCCTAQQQPQVPCYFIFGDSLVDNGNNNRLSSLARANYLPYGIDFPQGTTGR 72

Query: 82  FSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQL 134
           FSNGKTTVDVIAELLGFN YIPPYSNTRGRDILRGVNYASAAAGIREETGQQL
Sbjct: 73  FSNGKTTVDVIAELLGFNTYIPPYSNTRGRDILRGVNYASAAAGIREETGQQL 125

[Close]

BLAST of Cucsat.G2052.T7 vs. TAIR 10
Match: AT1G29670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 176.0 bits (445), Expect = 2.2e-44
Identity = 85/115 (73.91%), Postives = 96/115 (83.48%), Query Frame = 0


Query: 20  LALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGIDFPRGPT 79
           + L+L+   FS   AQ Q+QVPC+F+FGDSLVDNGNNN L S+AR++Y PYGIDF  GPT
Sbjct: 10  VVLVLLCFGFSVVKAQAQAQVPCFFVFGDSLVDNGNNNGLISIARSNYFPYGIDF-GGPT 69

Query: 80  GRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQL 135
           GRFSNGKTTVDVIAELLGFNGYIP Y+   GR IL GVNYASAAAGIREETG+QL
Sbjct: 70  GRFSNGKTTVDVIAELLGFNGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQL 123

[Close]

BLAST of Cucsat.G2052.T7 vs. TAIR 10
Match: AT4G18970.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 173.3 bits (438), Expect = 1.5e-43
Identity = 85/121 (70.25%), Postives = 95/121 (78.51%), Query Frame = 0


Query: 14  RPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGID 73
           R C   +A+ +   A + + A      PCYFIFGDSLVD+GNNNRL+SLARA+Y PYGID
Sbjct: 3   RVCVMMMAMAI---AMAMNIAMGDPIAPCYFIFGDSLVDSGNNNRLTSLARANYFPYGID 62

Query: 74  FPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQ 133
           F  GPTGRFSNGKTTVDVI ELLGF+ YI PYS  RG DILRGVNYASAAAGIREETG+Q
Sbjct: 63  FQYGPTGRFSNGKTTVDVITELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQ 120

Query: 134 L 135
           L
Sbjct: 123 L 120

[Close]

BLAST of Cucsat.G2052.T7 vs. TAIR 10
Match: AT4G18970.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 173.3 bits (438), Expect = 1.5e-43
Identity = 85/121 (70.25%), Postives = 95/121 (78.51%), Query Frame = 0


Query: 14  RPCSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGID 73
           R C   +A+ +   A + + A      PCYFIFGDSLVD+GNNNRL+SLARA+Y PYGID
Sbjct: 3   RVCVMMMAMAI---AMAMNIAMGDPIAPCYFIFGDSLVDSGNNNRLTSLARANYFPYGID 62

Query: 74  FPRGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQ 133
           F  GPTGRFSNGKTTVDVI ELLGF+ YI PYS  RG DILRGVNYASAAAGIREETG+Q
Sbjct: 63  FQYGPTGRFSNGKTTVDVITELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQ 120

Query: 134 L 135
           L
Sbjct: 123 L 120

[Close]

BLAST of Cucsat.G2052.T7 vs. TAIR 10
Match: AT5G45670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 9.4e-43
Identity = 82/116 (70.69%), Postives = 95/116 (81.90%), Query Frame = 0


Query: 20  LALLLITTAFSSSN-AQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGIDFPRGP 79
           L +++I  A +  N A+     PCYFIFGDSLVDNGNNN+L SLARA+Y PYGIDF  GP
Sbjct: 6   LMIMMIMVAVTMINIAKSDPIAPCYFIFGDSLVDNGNNNQLQSLARANYFPYGIDFAAGP 65

Query: 80  TGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQL 135
           TGRFSNG TTVDVIA+LLGF  YI PY++ RG+DILRGVNYASAAAGIR+ETG+QL
Sbjct: 66  TGRFSNGLTTVDVIAQLLGFEDYITPYASARGQDILRGVNYASAAAGIRDETGRQL 121

[Close]

BLAST of Cucsat.G2052.T7 vs. TAIR 10
Match: AT1G29660.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 166.4 bits (420), Expect = 1.8e-41
Identity = 82/119 (68.91%), Postives = 94/119 (78.99%), Query Frame = 0


Query: 16  CSFFLALLLITTAFSSSNAQQQSQVPCYFIFGDSLVDNGNNNRLSSLARADYLPYGIDFP 75
           C   + +LL+   F     + + QVPCYFIFGDSLVDNGNNNRL S+ARADY PYGIDF 
Sbjct: 9   CLVSVWVLLLGLGF---KVKAEPQVPCYFIFGDSLVDNGNNNRLRSIARADYFPYGIDF- 68

Query: 76  RGPTGRFSNGKTTVDVIAELLGFNGYIPPYSNTRGRDILRGVNYASAAAGIREETGQQL 135
            GPTGRFSNG+TTVDV+ ELLGF+ YIP YS   G++IL+GVNYASAAAGIREETG QL
Sbjct: 69  GGPTGRFSNGRTTVDVLTELLGFDNYIPAYSTVSGQEILQGVNYASAAAGIREETGAQL 123

The following BLAST results are available for this feature:

BLAST of Cucsat.G2052.T7 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7N4	3.2e-43	73.91	GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2...	[more]
Q93YW8	2.1e-42	70.25	GDSL esterase/lipase At4g18970 OS=Arabidopsis thaliana OX=3702 GN=At4g18970 PE=2...	[more]
Q9FK75	1.3e-41	70.69	GDSL esterase/lipase At5g45670 OS=Arabidopsis thaliana OX=3702 GN=At5g45670 PE=2...	[more]
Q9C7N5	2.5e-40	68.91	GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1...	[more]
Q8L5Z1	4.3e-40	67.83	GDSL esterase/lipase At1g33811 OS=Arabidopsis thaliana OX=3702 GN=At1g33811 PE=2...	[more]

BLAST of Cucsat.G2052.T7 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134596.1	9.98e-89	100.00	GDSL esterase/lipase At1g29670 [Cucumis sativus] >KGN49344.1 hypothetical protei...	[more]
XP_008439698.1	1.74e-79	92.59	PREDICTED: GDSL esterase/lipase At1g29670-like [Cucumis melo]	[more]
KAA0052587.1	1.74e-79	92.59	GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK13238.1 GDSL esterase/lipase...	[more]
XP_038881173.1	6.42e-71	85.82	GDSL esterase/lipase At1g29670-like [Benincasa hispida]	[more]
KAG6581389.1	2.15e-62	79.55	GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia] >KAG703467...	[more]

BLAST of Cucsat.G2052.T7 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKH4	4.83e-89	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520420 PE=3 SV=1	[more]
A0A5D3CPY8	8.40e-80	92.59	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255...	[more]
A0A1S3AYY6	8.40e-80	92.59	GDSL esterase/lipase At1g29670-like OS=Cucumis melo OX=3656 GN=LOC103484416 PE=3...	[more]
A0A6J1INT6	2.09e-62	79.55	GDSL esterase/lipase At1g29670-like OS=Cucurbita maxima OX=3661 GN=LOC111478592 ...	[more]
A0A6J1ECX6	1.12e-61	87.61	GDSL esterase/lipase At1g29670-like OS=Cucurbita moschata OX=3662 GN=LOC11143303...	[more]

BLAST of Cucsat.G2052.T7 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G29670.1	2.2e-44	73.91	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT4G18970.1	1.5e-43	70.25	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT4G18970.2	1.5e-43	70.25	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G45670.1	9.4e-43	70.69	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G29660.1	1.8e-41	68.91	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 43..123 e-value: 1.4E-11 score: 45.3
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 28..148 e-value: 5.2E-24 score: 87.2
None	No IPR available	PANTHER	PTHR45650	GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED	coord: 22..137
None	No IPR available	PANTHER	PTHR45650:SF60	GDSL-LIKE LIPASE/ACYLHYDROLASE SUPERFAMILY PROTEIN-RELATED	coord: 22..137

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G2052	Cucsat.G2052	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G2052.T7.E1	Cucsat.G2052.T7.E1	exon
Cucsat.G2052.T7.E2	Cucsat.G2052.T7.E2	exon
Cucsat.G2052.T7.E3	Cucsat.G2052.T7.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G2052.T7.C2	Cucsat.G2052.T7.C2	CDS
Cucsat.G2052.T7.C1	Cucsat.G2052.T7.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G2052.T7	Cucsat.G2052.T7-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds