Cucsat.G19774.T12 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G19774.T12
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Acyl-[acyl-carrier-protein] hydrolase
Location	ctg4: 924456 .. 928611 (+)
RNA-Seq Expression	Cucsat.G19774.T12
Synteny	Cucsat.G19774.T12

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TAGTTACCAAGATGCAGATTATGGTAGACCGTTACCCTACTTGGTAAATTTAAGTTCTTCCATGAAGTATTCGCTTCTAAAGATGGATTCATTATAACACTTGTGATTTACTTGGGTGGGTGAGAACAGAATTGAATTTAATTCAATAGTTTTCCTTGTTTGTTTTATCCCCTTAAGTTACATATCCAGTATTGCCCTGTTCCTTGGGATTATTTAACATATTCACCTCACTTTCACCTTCCAAAAATAATTGATTATGGAAACGTTGGACTTTCTAGACCAATAAGTCTATGAAGTGGGCTTTGATTTACACTTTTTTTTTATAAGGTGGAAGTTTTTGAAGCCTCCCTCTGTCTTTTCTGCTTTAGCTGATCTCTTCTTCCGTCTTTTATTAACTATATGCATGAGATATTGGAGATTGTTGTGCTTACAGGGGTGATACGGTTCAAGTAGACACTTGGGTCAGTGCTTCTGGAAAGAATGGCATGCGCCGTGATTGGCTCGTGTATGATTGCAAAACTAGGGAAACTTTGACAAGAGCTTCCAGGTTTGTTACTTTCCCCCTTTTTGATGAAACATGGAATCGTTTTTCTTCATAGCTGTTGCATTTTCTTTTTTCTCAACCCATGAATTATAAAGTTAACCAGCTTTGATTGAATCGATAATCGAGTAATTCTGGTCATAGGTGATGTAGGTTCTTGTATAAGTTATGAATTTCTAATGTGTGTTCCGTAGAGAACAGAAACAAAATTTGCATAAACTACATTCACTTTTTTTTCAATCTTAATTCTAATTGATCTTTGCATTGAGATCGTGGATTATGATTGCATTCTTTTTTTCTACTATCAAGCCATGGCATATGTAGTGATCAAATCATAGTTTACTGATAGAGATATATTTTTTGTGATGTGAAGTATCTGGGTTATGATGAATAAAGAGACAAGAAAGCTGTCAAAGATTCCTGAGGAAGTGAGGAGAGAAATAGAGCCATTCTTTATGAATACTGCGCCGGTTATTGAAGATGATGGAAGGAGGCTTCCAAAAATTGATGATGATAAAGCTGATTATGTTCGAGCGGGTTTGACTGTAAGTTGTTTCATTTAGCTAAAATGTTACTTGAGAAAAGGAAGTTGGCACTGGGTGTTTTTGTGTTTCATTTGGAATTTGGTTCAATAATATAACTTTCACTATGCTTTTGTACATAGAAATTGATCTCATGGCACCTACATTTCTGTACTGCAGCCTAAATGGAGTGATTTAGATGTCAACCAGCATGTGAACAATGTGAAGTACATTGGCTGGATTCTTGAGGTAGATAAATCTTAACTTTTCTTATCATTGACTTCGATACTTTCCGAAAATAGTGTGCCCCAAAATTTTCTTATCTCGCACTCATACATTTCTCCAACATTTTACACCGTAACTGTTCTTCTAAAGCTTGGTTTCCTCTTTTTGATCGGCAAATTATGCTTGTGGTCAATAGAGTGCCCCACCACCGATATTAGAGAGTCATGAACTTTCTTCCATCAATTTGGAGTATCGGAGGGAATGTGGGAGGGACAGTGTCCTGCAGTCACTCACTGCAGTTACTGGCACGGATGTTGGAACTTTAGCAACTTCTGGCGATGTTGACTGCAATCACCTACTTCGGTTCGAGAATGGGGCTGAGATTGTGAGAGGAAGAACAGAGTGGAGACCCAAATTGGGCAACAATTTTAGGATCCTGGATCATATTTCAGCTGATCAAGGAATTTAAGCTGCCATCTCAGAGGAGTGTTCCTGCTGTATCCATGGTTTGCACCAAATTCTGCTTTAAATCCTCAGATATATATGTCTATAAATATGGTTTTTCTCATTCGTTCTCTTCTATAAACAGGAAAAGAAAAAGGAAAAAAAAAAGGTTTAATTTCATTGTAATTATCTCTTATCTATTATTTTTCCCTCTTGTCCCCAGAAGGCTACTATATAATGTGTTTATCTTGCTCAGCCTGGTCGGTTGCTATGCAATCAATGTGTACCCACCGCTGGCTGATTGTCTCACAGCAGGTTCGAAAATATTTTTGTGTATATTTTCTTGCATTTTCTTTAATTTTTGTTTGGAACTGGAAAAAAAAAAAGAGAGATTTTTTCCCCATTTTTTTGCCCTCGAATTGTCGCTCTTCTGCACTCACTTTGCTTTGAGCAGATTGGAAAATATTCATAACTTGTGTTTGAAATTGGTTGGAAAAAGAAAAAAAGAACCCAGGGGTGATACTTGTATGTTTTCTTGCTGTTTCTTTGACTTCTCTTTGGCATTTTTAAATACCATTTTTCTCTCCCAGGAGTTCGAATTATCCAATTTTTTATGTGTGGAAAAAGCTGGGGTGTCTAGATTTTCGTCCCAGAATTATTTACTTTTGTGGTAGTGGAATTTGGTAAAAACTAAAAAGTGGCATTTATTAGTCTTTTAATGCTAGCTGGGTCACAGTTATTGTTTGTTTTGATTTGATGGCAATTACTAAAGCTCAATTATTGCACAAAATTTATGCCCATCTCAGTTGGTTAAAAAGCTGGATGAGTGAAGTGGTGGCTTTTATATTATTATTATTTGAAATTGATATTGCAATTTTCGTGCCTTTATAAAAGAATTCTTTGACTTTGACAAAATAGTTTCAAGAATTGGACATTGTGATTTTTGGCCATTGAGATGAAGTTCTCTATTAATTGTCTTCTTTAATAATATAAAGTTTTGTTTAAAAAACATTATATATATACATATATTTCTTAAATTAAAAGGTGGCTTTATAATTGGATTTTTTTTTTGTCGTCATTATTATTCTTTGGATACTATAATCTTACTATAGAGGTGATTTCTAGTCTCCGTAGTTAATTCTTTAAAAGATATAATTATTGGAGTGGGACAGAAGTTGGATGAACACGTTCTAATTAAAAAGTTTTGTTTGATTTTATAAAAAAAGTGTCTAGTTATTGGGATGGTTCTTAAATTGTAAACTTGAAAATAATTATCCAACAAAAGTGGTAAGGATATTGAGTTTGCTTAATTAAATAATAACCTCCATAGTCCCATTTTTACCTAAATGCGAAACTAGCTAAATATTACATAATAAAGTTAATGTGGAAAGTAGAATTTCTTCAACTTTTAACTGCGAACAATGATCGGAAAGACTATTCTCATGTGAAATGACCTAATTCTAGGGAAGTCTCGAAGTTTTGATGCAATGTTCTTCTAAATCACTAGTCGACCAATCAAGGTTGTAGGAGTTCTCAGGGTGATGATTTAATTTTCATTGGTTTTTCTGGAGTTGGGTCCTTTCGCGTTAACAGCTTCATGATTGGAGAAAAAAAATAAACTTATTTGCCTCACCTTGAGGTAAATACGTTTGAGTTGATATAGTGGAGGCCTCTGTCTCACCACTAGTTTTTAGAAACCCAAAGTATTTGAAACTTACTATTAAATTGAAGCCCAACTAAAAGTATACAAAAATTGTGTACATATTTATATATAAAATTAATACATAATGTAAGAAGGGTCCAATAATGAGAAACATCAAAAGTGTATCTCCATGTGCAAAAAGGCAAGAAATGAAACTGCCCATCTGCTGCTCAGTGTGAACTAGGAAGTTATTAGTCAGATTGGATGAGTTTGAATGCATTACCTCCGTTTTCTTCAAAAAAGAAAAGAAAAAGAAAAGAAAACCCTTTTTTTAAAAAAAAATTATTATCTCTATTTTCATTTCCCATTAAGAATTTATTTCTAAAAAAATTGATACAAACCTTAATAAAAGTAACAAATATCAACATGTCTTCGGGACGATTCGTACATTGGTGATAAACTTCTATTATTGTATCATTGATAGACTCGTTTTATATGTTAATATTTTGAATTTAATAACTATACATGACAATTGCACCACTAATTAATGATTTTATTTTTCTTTTTAATAATGTAGGAGCAGGAGATGAAATCTTAAACATCAAGATTAAGAATGTTAAAGCTATATTCATGTCATTTGTTATAGCTTAGTAGATTAATTATGGATATCTAATTATCAAAAGAAATAGGAGTGAATAATATCGTCAAAACCACCCACAACTCTATGTAATAATAATATTTTCTTAAAAAGAAGCCTATGGTATAGTTAATTACATTAAGAAG

Coding sequence (CDS)

AGTTACCAAGATGCAGATTATGATTGTTGTGCTTACAGGGGTGATACGGTTCAAGTAGACACTTGGGTCAGTGCTTCTGGAAAGAATGGCATGCGCCGTGATTGGCTCGTGTATGATTGCAAAACTAGGGAAACTTTGACAAGAGCTTCCAGTATCTGGGTTATGATGAATAAAGAGACAAGAAAGCTGTCAAAGATTCCTGAGGAAGTGAGGAGAGAAATAGAGCCATTCTTTATGAATACTGCGCCGGTTATTGAAGATGATGGAAGGAGGCTTCCAAAAATTGATGATGATAAAGCTGATTATGTTCGAGCGGGTTTGACTCCTAAATGGAGTGATTTAGATGTCAACCAGCATGTGAACAATGTGAAGTACATTGGCTGGATTCTTGAGAGTGCCCCACCACCGATATTAGAGAGTCATGAACTTTCTTCCATCAATTTGGAGTATCGGAGGGAATGTGGGAGGGACAGTGTCCTGCAGTCACTCACTGCAGTTACTGGCACGGATGTTGGAACTTTAGCAACTTCTGGCGATGTTGACTGCAATCACCTACTTCGGTTCGAGAATGGGGCTGAGATTGTGAGAGGAAGAACAGAGTGGAGACCCAAATTGGGCAACAATTTTAGGATCCTGGATCATATTTCAGCTGATCAAGGAATTTAA

Protein sequence

SYQDADYDCCAYRGDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRREIEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESAPPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAEIVRGRTEWRPKLGNNFRILDHISADQGI

Homology

[Close]

BLAST of Cucsat.G19774.T12 vs. ExPASy Swiss-Prot
Match: Q9SJE2 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FATB PE=1 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.4e-84
Identity = 146/191 (76.44%), Postives = 166/191 (86.91%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GD V+VDTWVS SGKNGMRRDWLV DC T ETLTRASS+WVMMNK TR+LSKIPEEVR E
Sbjct: 212 GDVVEVDTWVSQSGKNGMRRDWLVRDCNTGETLTRASSVWVMMNKLTRRLSKIPEEVRGE 271

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEP+F+N+ PV+ +D R+L KIDD  ADYVR+GLTP+WSDLDVNQHVNNVKYIGWILESA
Sbjct: 272 IEPYFVNSDPVLAEDSRKLTKIDDKTADYVRSGLTPRWSDLDVNQHVNNVKYIGWILESA 331

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           P  I+E  +L S+ LEYRRECGRDSVLQSLTAVTG D+G LAT+GDV+C HLLR ++GAE
Sbjct: 332 PVGIMERQKLKSMTLEYRRECGRDSVLQSLTAVTGCDIGNLATAGDVECQHLLRLQDGAE 391

Query: 194 IVRGRTEWRPK 205
           +VRGRTEW  K
Sbjct: 392 VVRGRTEWSSK 402

[Close]

BLAST of Cucsat.G19774.T12 vs. ExPASy Swiss-Prot
Match: Q9SQI3 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum OX=3635 GN=FATB1 PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 1.6e-80
Identity = 142/204 (69.61%), Postives = 167/204 (81.86%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GD VQVDTWVSASGKNGMRRDWLV + +T E LTRA+S+WVMMNK TR+LSKIPEEVR E
Sbjct: 207 GDVVQVDTWVSASGKNGMRRDWLVSNSETGEILTRATSVWVMMNKLTRRLSKIPEEVRGE 266

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEPFFMN+ PV+ +D ++L K+DD  A++V  GLTPKWSDLDVNQHVNNVKYIGWILESA
Sbjct: 267 IEPFFMNSDPVLAEDSQKLVKLDDSTAEHVCKGLTPKWSDLDVNQHVNNVKYIGWILESA 326

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           P PILESHELS++ LEYRRECGRDSVLQSLT V+ ++       G+ +C HLLR ++GAE
Sbjct: 327 PLPILESHELSALTLEYRRECGRDSVLQSLTTVSDSNTENAVNVGEFNCQHLLRLDDGAE 386

Query: 194 IVRGRTEWRPKLGNNFRILDHISA 218
           IVRGRT WRPK   +   +D I+A
Sbjct: 387 IVRGRTRWRPKHAKSSANMDQITA 410

[Close]

BLAST of Cucsat.G19774.T12 vs. ExPASy Swiss-Prot
Match: G3ESV0 (Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea viscosissima OX=857185 GN=FATB2 PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 4.6e-75
Identity = 132/191 (69.11%), Postives = 157/191 (82.20%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTV+V+TWV+ SGKNGMRRDWL+ DC T E LTRASS+WVMMN++TRKLSKIP+EVRRE
Sbjct: 208 GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRRE 267

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEP F+++APVIEDD R+LPK+D+  AD +R GLTP+W+DLDVNQHVNN KYIGWILES 
Sbjct: 268 IEPHFVDSAPVIEDDDRKLPKLDEKSADSIRKGLTPRWNDLDVNQHVNNAKYIGWILEST 327

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PP +LE+ EL S+ LEYRRECGR+SVL+SLTAV  +  G           HLLR E+G E
Sbjct: 328 PPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGY-----GSQFQHLLRLEDGGE 387

Query: 194 IVRGRTEWRPK 205
           IV+GRTEWRPK
Sbjct: 388 IVKGRTEWRPK 393

[Close]

BLAST of Cucsat.G19774.T12 vs. ExPASy Swiss-Prot
Match: Q39513 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana OX=36775 GN=FATB1 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 8.7e-74
Identity = 130/193 (67.36%), Postives = 157/193 (81.35%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTV+V+TWV+ SGKNGMRRDWL+ DC T E LTRASS+WVMMN++TR+LSKIP+EVR E
Sbjct: 211 GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNE 270

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEP F+++ PVIEDD R+LPK+D+  AD +R GLTP+W+DLDVNQHVNNVKYIGWILES 
Sbjct: 271 IEPHFVDSPPVIEDDDRKLPKLDEKTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILEST 330

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PP +LE+ EL S+ LEYRRECGR+SVL+SLTA+  +  G           HLLR E+G E
Sbjct: 331 PPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSGGGY-----GSQFQHLLRLEDGGE 390

Query: 194 IVRGRTEWRPKLG 207
           IV+GRTEWRPK G
Sbjct: 391 IVKGRTEWRPKNG 398

[Close]

BLAST of Cucsat.G19774.T12 vs. ExPASy Swiss-Prot
Match: Q41635 (Dodecanoyl-[acyl-carrier-protein] hydrolase, chloroplastic OS=Umbellularia californica OX=3438 GN=FATB1 PE=1 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 4.8e-64
Identity = 113/205 (55.12%), Postives = 153/205 (74.63%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTV+V+ W+ ASG NGMRRD+LV DCKT E LTR +S+ V+MN  TR+LS IP+EVR E
Sbjct: 180 GDTVEVECWIGASGNNGMRRDFLVRDCKTGEILTRCTSLSVLMNTRTRRLSTIPDEVRGE 239

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           I P F++   V +D+ ++L K++D  ADY++ GLTP+W+DLDVNQHVNN+KY+ W+ E+ 
Sbjct: 240 IGPAFIDNVAVKDDEIKKLQKLNDSTADYIQGGLTPRWNDLDVNQHVNNLKYVAWVFETV 299

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           P  I ESH +SS  LEYRREC RDSVL+SLT V+G      ++   + C+HLL+ E G+E
Sbjct: 300 PDSIFESHHISSFTLEYRRECTRDSVLRSLTTVSGG-----SSEAGLVCDHLLQLEGGSE 359

Query: 194 IVRGRTEWRPKLGNNFRILDHISAD 219
           ++R RTEWRPKL ++FR +  I A+
Sbjct: 360 VLRARTEWRPKLTDSFRGISVIPAE 379

[Close]

BLAST of Cucsat.G19774.T12 vs. NCBI nr
Match: XP_004139335.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic [Cucumis sativus] >XP_011648667.1 palmitoyl-acyl carrier protein thioesterase, chloroplastic [Cucumis sativus] >XP_031737012.1 palmitoyl-acyl carrier protein thioesterase, chloroplastic [Cucumis sativus] >KGN60640.1 hypothetical protein Csa_019438 [Cucumis sativus])

HSP 1 Score: 431 bits (1109), Expect = 3.25e-149
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE
Sbjct: 213 GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 272

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA
Sbjct: 273 IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 332

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE
Sbjct: 333 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 392

Query: 194 IVRGRTEWRPKLGNNFRILDHISADQGI 221
           IVRGRTEWRPKLGNNFRILDHISADQGI
Sbjct: 393 IVRGRTEWRPKLGNNFRILDHISADQGI 420

[Close]

BLAST of Cucsat.G19774.T12 vs. NCBI nr
Match: XP_008458039.1 (PREDICTED: palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucumis melo] >XP_008458045.1 PREDICTED: palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucumis melo] >XP_008458051.1 PREDICTED: palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucumis melo] >XP_008458060.1 PREDICTED: palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 425 bits (1093), Expect = 8.21e-147
Identity = 204/208 (98.08%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASS+WVMMNKETRKLSKIPEEVRRE
Sbjct: 211 GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSVWVMMNKETRKLSKIPEEVRRE 270

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEPFFMNTAPVIEDDGRRLPKIDDD ADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA
Sbjct: 271 IEPFFMNTAPVIEDDGRRLPKIDDDTADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 330

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PPPILESHELSSINLEYRRECGRDSV+QSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE
Sbjct: 331 PPPILESHELSSINLEYRRECGRDSVVQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 390

Query: 194 IVRGRTEWRPKLGNNFRILDHISADQGI 221
           IVRGRTEWRPKLGNNF ILDHISADQGI
Sbjct: 391 IVRGRTEWRPKLGNNFMILDHISADQGI 418

[Close]

BLAST of Cucsat.G19774.T12 vs. NCBI nr
Match: KAA0061575.1 (palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK10699.1 palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa])

HSP 1 Score: 424 bits (1090), Expect = 2.35e-146
Identity = 203/208 (97.60%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASS+WVMMNKETRKLSKIPEEVRRE
Sbjct: 211 GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSVWVMMNKETRKLSKIPEEVRRE 270

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEPFFMNTAPVIEDDGRRLPKIDDD ADYVRAGLTPKWSDLDVNQHVNNVKYIGWILE+A
Sbjct: 271 IEPFFMNTAPVIEDDGRRLPKIDDDTADYVRAGLTPKWSDLDVNQHVNNVKYIGWILETA 330

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PPPILESHELSSINLEYRRECGRDSV+QSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE
Sbjct: 331 PPPILESHELSSINLEYRRECGRDSVVQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 390

Query: 194 IVRGRTEWRPKLGNNFRILDHISADQGI 221
           IVRGRTEWRPKLGNNF ILDHISADQGI
Sbjct: 391 IVRGRTEWRPKLGNNFMILDHISADQGI 418

[Close]

BLAST of Cucsat.G19774.T12 vs. NCBI nr
Match: XP_038890785.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Benincasa hispida] >XP_038890786.1 palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 408 bits (1049), Expect = 4.40e-140
Identity = 197/208 (94.71%), Postives = 200/208 (96.15%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTVQVDTWVSASGKNGMRRDWLVYDCKT +TLTRASSIWVMMNKETRKLSKIPEEVR E
Sbjct: 214 GDTVQVDTWVSASGKNGMRRDWLVYDCKTGDTLTRASSIWVMMNKETRKLSKIPEEVRIE 273

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEPFFMNTAPVIEDDGRRLPKIDDD ADYVR GLTPKWSDLDVNQHVNNVKYIGWILESA
Sbjct: 274 IEPFFMNTAPVIEDDGRRLPKIDDDTADYVRTGLTPKWSDLDVNQHVNNVKYIGWILESA 333

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PPPILESHELSSI LEYRRECGRDSVLQSLTAVTG DVGTLAT+ DVDCNHLLRFENGAE
Sbjct: 334 PPPILESHELSSITLEYRRECGRDSVLQSLTAVTGMDVGTLATASDVDCNHLLRFENGAE 393

Query: 194 IVRGRTEWRPKLGNNFRILDHISADQGI 221
           IVRGRTEWRPKLGNNFRIL HISAD+GI
Sbjct: 394 IVRGRTEWRPKLGNNFRILGHISADRGI 421

[Close]

BLAST of Cucsat.G19774.T12 vs. NCBI nr
Match: XP_022158833.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 389 bits (998), Expect = 2.45e-132
Identity = 183/208 (87.98%), Postives = 197/208 (94.71%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GD VQVDTWVSASGKNGMRRDWLVYDCKT +TLTRASSIWVMMNK+TRKLSKIPEEVRRE
Sbjct: 214 GDAVQVDTWVSASGKNGMRRDWLVYDCKTGDTLTRASSIWVMMNKQTRKLSKIPEEVRRE 273

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEP+FMN+ PVIE+DGR+L K+DDD AD  R GLTPKWSDLDVNQHVNNVKYIGWILESA
Sbjct: 274 IEPYFMNSPPVIEEDGRKLSKVDDDTADNARMGLTPKWSDLDVNQHVNNVKYIGWILESA 333

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PPPILESHELSSI LEYRRECG+DSV+QSLTAVTGTDVGTLAT+GDV+C+HLLRFENGAE
Sbjct: 334 PPPILESHELSSITLEYRRECGKDSVIQSLTAVTGTDVGTLATAGDVECSHLLRFENGAE 393

Query: 194 IVRGRTEWRPKLGNNFRILDHISADQGI 221
           IVRGRTEWRPK+G+NFRILDHISAD  I
Sbjct: 394 IVRGRTEWRPKIGSNFRILDHISADHSI 421

[Close]

BLAST of Cucsat.G19774.T12 vs. ExPASy TrEMBL
Match: A0A0A0LF34 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G005370 PE=3 SV=1)

HSP 1 Score: 431 bits (1109), Expect = 1.57e-149
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE
Sbjct: 213 GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 272

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA
Sbjct: 273 IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 332

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE
Sbjct: 333 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 392

Query: 194 IVRGRTEWRPKLGNNFRILDHISADQGI 221
           IVRGRTEWRPKLGNNFRILDHISADQGI
Sbjct: 393 IVRGRTEWRPKLGNNFRILDHISADQGI 420

[Close]

BLAST of Cucsat.G19774.T12 vs. ExPASy TrEMBL
Match: A0A1S3C7I1 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo OX=3656 GN=LOC103497577 PE=3 SV=1)

HSP 1 Score: 425 bits (1093), Expect = 3.97e-147
Identity = 204/208 (98.08%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASS+WVMMNKETRKLSKIPEEVRRE
Sbjct: 211 GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSVWVMMNKETRKLSKIPEEVRRE 270

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEPFFMNTAPVIEDDGRRLPKIDDD ADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA
Sbjct: 271 IEPFFMNTAPVIEDDGRRLPKIDDDTADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 330

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PPPILESHELSSINLEYRRECGRDSV+QSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE
Sbjct: 331 PPPILESHELSSINLEYRRECGRDSVVQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 390

Query: 194 IVRGRTEWRPKLGNNFRILDHISADQGI 221
           IVRGRTEWRPKLGNNF ILDHISADQGI
Sbjct: 391 IVRGRTEWRPKLGNNFMILDHISADQGI 418

[Close]

BLAST of Cucsat.G19774.T12 vs. ExPASy TrEMBL
Match: A0A5A7V6V4 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00080 PE=3 SV=1)

HSP 1 Score: 424 bits (1090), Expect = 1.14e-146
Identity = 203/208 (97.60%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASS+WVMMNKETRKLSKIPEEVRRE
Sbjct: 211 GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSVWVMMNKETRKLSKIPEEVRRE 270

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEPFFMNTAPVIEDDGRRLPKIDDD ADYVRAGLTPKWSDLDVNQHVNNVKYIGWILE+A
Sbjct: 271 IEPFFMNTAPVIEDDGRRLPKIDDDTADYVRAGLTPKWSDLDVNQHVNNVKYIGWILETA 330

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PPPILESHELSSINLEYRRECGRDSV+QSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE
Sbjct: 331 PPPILESHELSSINLEYRRECGRDSVVQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 390

Query: 194 IVRGRTEWRPKLGNNFRILDHISADQGI 221
           IVRGRTEWRPKLGNNF ILDHISADQGI
Sbjct: 391 IVRGRTEWRPKLGNNFMILDHISADQGI 418

[Close]

BLAST of Cucsat.G19774.T12 vs. ExPASy TrEMBL
Match: A0A6J1DX70 (Acyl-[acyl-carrier-protein] hydrolase OS=Momordica charantia OX=3673 GN=LOC111025297 PE=3 SV=1)

HSP 1 Score: 389 bits (998), Expect = 1.18e-132
Identity = 183/208 (87.98%), Postives = 197/208 (94.71%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GD VQVDTWVSASGKNGMRRDWLVYDCKT +TLTRASSIWVMMNK+TRKLSKIPEEVRRE
Sbjct: 214 GDAVQVDTWVSASGKNGMRRDWLVYDCKTGDTLTRASSIWVMMNKQTRKLSKIPEEVRRE 273

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEP+FMN+ PVIE+DGR+L K+DDD AD  R GLTPKWSDLDVNQHVNNVKYIGWILESA
Sbjct: 274 IEPYFMNSPPVIEEDGRKLSKVDDDTADNARMGLTPKWSDLDVNQHVNNVKYIGWILESA 333

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PPPILESHELSSI LEYRRECG+DSV+QSLTAVTGTDVGTLAT+GDV+C+HLLRFENGAE
Sbjct: 334 PPPILESHELSSITLEYRRECGKDSVIQSLTAVTGTDVGTLATAGDVECSHLLRFENGAE 393

Query: 194 IVRGRTEWRPKLGNNFRILDHISADQGI 221
           IVRGRTEWRPK+G+NFRILDHISAD  I
Sbjct: 394 IVRGRTEWRPKIGSNFRILDHISADHSI 421

[Close]

BLAST of Cucsat.G19774.T12 vs. ExPASy TrEMBL
Match: A0A6J1G3K8 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450443 PE=3 SV=1)

HSP 1 Score: 374 bits (959), Expect = 8.34e-127
Identity = 179/208 (86.06%), Postives = 188/208 (90.38%), Query Frame = 0


Query: 14  GDTVQVDTWVSASGKNGMRRDWLVYDCKTRETLTRASSIWVMMNKETRKLSKIPEEVRRE 73
           GDTVQVDTWVSASG+NGMRRDWLVYDCKT + LTRASS WVMMNK+TRKLSKIPEEVRRE
Sbjct: 209 GDTVQVDTWVSASGRNGMRRDWLVYDCKTGDNLTRASSTWVMMNKQTRKLSKIPEEVRRE 268

Query: 74  IEPFFMNTAPVIEDDGRRLPKIDDDKADYVRAGLTPKWSDLDVNQHVNNVKYIGWILESA 133
           IEP FMNTAPVIEDDGRRL KIDDD ADY R GLTPKWSDLDVNQHVNNVKYIGWILESA
Sbjct: 269 IEPNFMNTAPVIEDDGRRLSKIDDDTADYFRMGLTPKWSDLDVNQHVNNVKYIGWILESA 328

Query: 134 PPPILESHELSSINLEYRRECGRDSVLQSLTAVTGTDVGTLATSGDVDCNHLLRFENGAE 193
           PPPILESHELSSI LEYRRECGRDSVLQSLTA TGTD  T +T+G VD NHLLRF++G E
Sbjct: 329 PPPILESHELSSITLEYRRECGRDSVLQSLTAATGTDSATSSTAGVVDYNHLLRFDDGTE 388

Query: 194 IVRGRTEWRPKLGNNFRILDHISADQGI 221
           +VRGRT+WRPK GN F ILDHISAD GI
Sbjct: 389 VVRGRTQWRPKFGNKFTILDHISADHGI 416

The following BLAST results are available for this feature:

BLAST of Cucsat.G19774.T12 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SJE2	1.4e-84	76.44	Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thalia...	[more]
Q9SQI3	1.6e-80	69.61	Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum...	[more]
G3ESV0	4.6e-75	69.11	Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea ...	[more]
Q39513	8.7e-74	67.36	Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana ...	[more]
Q41635	4.8e-64	55.12	Dodecanoyl-[acyl-carrier-protein] hydrolase, chloroplastic OS=Umbellularia calif...	[more]

BLAST of Cucsat.G19774.T12 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139335.1	3.25e-149	100.00	palmitoyl-acyl carrier protein thioesterase, chloroplastic [Cucumis sativus] >XP...	[more]
XP_008458039.1	8.21e-147	98.08	PREDICTED: palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucu...	[more]
KAA0061575.1	2.35e-146	97.60	palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK10699...	[more]
XP_038890785.1	4.40e-140	94.71	palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Benincasa hispi...	[more]
XP_022158833.1	2.45e-132	87.98	palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Momordica chara...	[more]

BLAST of Cucsat.G19774.T12 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LF34	1.57e-149	100.00	Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G005370...	[more]
A0A1S3C7I1	3.97e-147	98.08	Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo OX=3656 GN=LOC103497577 PE...	[more]
A0A5A7V6V4	1.14e-146	97.60	Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1DX70	1.18e-132	87.98	Acyl-[acyl-carrier-protein] hydrolase OS=Momordica charantia OX=3673 GN=LOC11102...	[more]
A0A6J1G3K8	8.34e-127	86.06	Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002864	Acyl-ACP thioesterase	PFAM	PF01643	Acyl-ACP_TE	coord: 14..202 e-value: 1.7E-53 score: 181.5
None	No IPR available	GENE3D	3.10.129.10	Hotdog Thioesterase	coord: 1..207 e-value: 1.6E-67 score: 229.4
None	No IPR available	PANTHER	PTHR31727:SF2	PALMITOYL-ACYL CARRIER PROTEIN THIOESTERASE, CHLOROPLASTIC	coord: 14..207
IPR045023	Acyl-[acyl-carrier-protein] hydrolase FATA/B	PANTHER	PTHR31727	OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTIC	coord: 14..207
IPR029069	HotDog domain superfamily	SUPERFAMILY	54637	Thioesterase/thiol ester dehydrase-isomerase	coord: 5..87
IPR029069	HotDog domain superfamily	SUPERFAMILY	54637	Thioesterase/thiol ester dehydrase-isomerase	coord: 102..201

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G19774	Cucsat.G19774	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G19774.T12.E1	Cucsat.G19774.T12.E1	exon
Cucsat.G19774.T12.E2	Cucsat.G19774.T12.E2	exon
Cucsat.G19774.T12.E3	Cucsat.G19774.T12.E3	exon
Cucsat.G19774.T12.E4	Cucsat.G19774.T12.E4	exon
Cucsat.G19774.T12.E5	Cucsat.G19774.T12.E5	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G19774.T12.C1	Cucsat.G19774.T12.C1	CDS
Cucsat.G19774.T12.C2	Cucsat.G19774.T12.C2	CDS
Cucsat.G19774.T12.C3	Cucsat.G19774.T12.C3	CDS
Cucsat.G19774.T12.C4	Cucsat.G19774.T12.C4	CDS
Cucsat.G19774.T12.C5	Cucsat.G19774.T12.C5	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G19774.T12	Cucsat.G19774.T12-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0016297	acyl-[acyl-carrier-protein] hydrolase activity
molecular_function	GO:0016790	thiolester hydrolase activity
molecular_function	GO:0000036	acyl carrier activity