Cucsat.G16310.T1 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G16310.T1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	protein STRICTOSIDINE SYNTHASE-LIKE 10-like
Location	ctg2246: 73066 .. 78963 (-)
RNA-Seq Expression	Cucsat.G16310.T1
Synteny	Cucsat.G16310.T1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGAGAGTTTAAAAATATGTGACTAACTGAAACTTTGAAGTAATATATATTATGGGGAGAGGTAATTATTCTAGTTATAGATAATTTTTATCCCAACTCTAAATATTTATATGGGAGAGAATGAAAAGAAAGGGTAAAAATATAGATTATATATAGAGAGAGATGATGGGGGAGGAAATTAAAGTAGGTGAGAGAGTGAATAAGAGGGCATTAACTCTTAAGTATAGTGGAAGGAATAATGAAGAAGTGTGTGGGAGTTTGTTCATTGTTGGGTGCGGTGGCCGTTCTGTGTGTGGTGGCGGTGGTGGAAGGTGCAATTGAAGCCGTAGAGCTTCCCGGTGGTGTTTTCGGGCCGGAAAGTATAGCCTTTGACTGCCGTGGAGAAGGGCCCTACGCCAGTGTCTCCGATGGCAGAATCCTCAAGTGGAAAGGTCCTCACTTGGGTTGGACTCAATTTGCTCTTACCTCACCCAACAGGTATAATATTATATACCCAAACCTTTCACTTTTCTTTTCTTTTTTCCACTAAATTATAACAAATAATTTAAAATTTACACTATTATCCTCCCACTTTTATAAACATTTTTAAACTATTCTTTTGAAATTTTTAGGACTTCAAATGATGGGGCTTTGACCTAAACTAAAACGATGCGGTTGATTCGCATCTTTTTCAGTTTTTTTATTATATACAACACCAATTTATATCTCAACTTTCGTTCAAGTTTTGAATTTTTTTTATTCCAACCATGTTTTAGAAATGTTTATGGAAGTTTAACCATATTATTTCAACTTTTGAAATTATAGAGATATTATTTAATTAAAAAGTCAAAAAGTTTTTAAGAGTTTTTTTCTTCTTTAAATGATCATTCTTTAGATAAAAATAAAAAAAATAAAAAAATAAAATATGTGTTGTTAATCTCGATGTCAATAGTTTGAACTAGGCGTTGAACTAAAATAATGAAAATGACTAACCTACCATTGGATACCTATTTGGTTTATTTTTATTTAAGAATATATTGTTTGAAATAGTTATTCAACCAAAAAAAAATGGATGGGCAGACTTGTATGGTTGGAAAAAAAACTTTATAAAACATAAAAGTAAAAAAAAGTTATTGTGAATACCGGATGTGTTAAAAATATTTACACCATACACCAAAATTTAGATTTTTAAATCTATCAATAATAAAATTCTGAAATTTACTATATTTTATAAATATTTAAATTCATTAGATTAGGTTTAAAAATATTAAAAAAAGTTTTTAAATGACATTTATCTAAGAAGTTTTTTTCAAAAATAGAAAAATCTACAAACTATTTACATTCTACCTAACAAAACTATAAAAAAAAAAAAAAAAATTTTAGAAAGGGTAAATATTTGGTCAAATATTCTATTTTTACCCCACCTACCTGCTTTTCTTTACTTTCTACCAACAGATCAATGTAATAAACATTTTAATTATTTTGCAAACAATAAAAGGCTTATTTATTAATTTAAATATATTCATTTACTTTAATAATCTTTCATGGAGTTTTTACATCAATATCTAGCCAATATATTTGGCAAGACAAGAACACACCATTATTTAATTGTAGGCATTTTCCTTATTTACCCCTATCTATATTAATGGTTGGTATATCATATTAGAGCAGAGGCCAGAAGTTTGTGCATAAAGAGGAAAAAGCTTTCCCATTACAACTTTTTCTGACTTCTTATAAATTCACAGATCTTCCGATTTTAAATTATTAGGTACACACAATATATATCTTTTGTTATTTTGCAAATTTCAATCAGTTCCCACAAAGTTTCAATAATTGGTTTCTACGAGATTTGGTTAAAGCAAAAATAGAAAGCTGAAATATTTTGTGAGATCTTAAGTAAAATTAAATTTAAGGAACCAACTAAAACAAAATTCAAATTACAAGCAAGAATTTTAATGGAAAATATAAGATCAAAATGGTCAAATCATTGATGGAACTAAAAGCATCTAGTCAAACTTGTGAGTTTGAAAGCTTTTGAAAGACCAATTGGAAAAAAAAGGGCATTATAGGATTTGGAATCACTTTTAAAATCAATTACGGGGCTGACCTTACTATTTTATTAAGATTTTGACTTGGTTGATTTTTCTATTAGGAGATTCTTAATGTCGGATTTCATTTGTGTTCATCATTCTTTGATAAGATCCTAAAGTTTGTTATTTTTTGGATCAAATGTCGGCTCATTGACTTTTATGAACTTTGATATCATATAATTAGATATGATAATTGCAATAAGTAACGTAATAATTAAGAAGAGTTACAAATATAAAAAAATTCATAAGTGATTGACTCTATAACAGATAATCTGTCATAAATGGTCACTATGTTGCTGATAGATTTTTAATAGACTTGGGAGTTTATTTGATTGAAAATTATATTAAAATACTAATTTGAGCTTGATTGCTATATTTGCAACCATCTTGTTAGGTGAAACAAGATTTCATTTCAAAACCAATTCGACAATGAGAGAAACAATAATCCATCGAATCTGATTAAGATTTTGTGAGGTTTCTTAAGTTTTGAATAACATTATATTATATTGATAGTGGGATGTTGGAAGATAACATGATGCACGATAAAAGAAAATTATACACGCATGATGCAACATGTTGACCAGATTCTTCAACGGAATCATAGAAGAAGAAATTAAATTCAGAAACAAAAGTCTTAAAGATGAATGGGATGTTAGAAGGTAGAGAATGATATGGAGTGTTTTTTGTGTTGTTGTTTTCAAAAGGGAAGGGAAAGAATGCGATGGGCAGCCCCAAAGTGAGGCAGCATGTGGAAGGCCATTAGGGATCAAATTTCACCCTACAACTTGTGACCTTTACATTGCTGATGCTTATTTTGGCCTTCTGGCTGTAGGACCTAAGGGTGGCTTGGCTCGCCAGCTTGCCACTTCTGCTCAAGGCGTGCCACTTCGCTTCACTAATGCTCTTGATATTGATCCTCAAAATGGCATTGTTTATTTCACCGATAGTAGCATCCTCTTTCAAAGAAGGTAATCTAATCTAATCTATGTCTCAAAATTTTAGTTTTTACTTTTGCTAACTTACTTACTATTTATCTTTGAATGGTATGATATTTTGGTATGTTTATAAATATTTTCAAAATTTTTATTATTTAAGATAAAATTTCATATATATTATGTGTGTATAATATATATATTTTGTACTCATTTAAACTTAAAAATATTTAATTTTATACTTTTTTTCTCTCCTTTCTTATTCCTTCTTTTCCTTGTCTCTGCCTCCTTCCAACGTTTTTCTTCATGGTTACCACTGTCACTAGCCATTGTCCACCACAATACTTCTTTTCTTCTCACTCTTTCCCTTTCTCTTTTTCCTTATTTTCTCCTCTACTGGGGCGAGAAAGAAAGAAGAGAGAAAATAGTGGTGGGATATAATAATGGTCATCGAATTGATGGCGGTGAACAACAAACAGTGGCAATGACAGCCATAAAGAAAGAAGGTTGAAGAAAGAGATGGATAAAAGAAGAAGAAAGAGGGAAAAGAAAGAAATATCATAAATTTAATTTTGGTTCCAAAAAAATGTGTTATACTTCATATTGATATATAAAGTGTTGAAATGTTTTTGTTTTAGTCAACTATATACATATATATATATGAGTTTATTGTTTATATGACAAATAATATTTTAATATTCCAAATAATAATTAAAATAGCCCTTGGTTTTCTTTTTCTTTTTTCGTTTCATGGAAATACTAAACATTAATATTGCAATAATTATACCTCTAGTTTGTTGTTTTCTTTCGAAGTTGTAACTTTAAATACTATTTTGTTAAGAGTATAAAATAAAAAATATTATTAAATGCCATTTAATAAATGAAGTTTGACATTTTCCAAACCAAAAAAGCATTCCACAAAATGTGGATGGATACAAAGAAAAATAGGGTATAGCTTTTATGTGTATTTATTTATGAAAAATAGTTGGTCGGATTGGGTTATACCTATTTCTATGCAATTCCCCATTTACTAAATTAAAAAGTAAATTATTATATTTTTGTTAGGTAAATGAGGGACCAAGTTGTGATTAATAATTATTGTCTCTTATATTTTACGTAGCAAAAAGAGTATAATAATTTAAAGTTTTTAAAATTTTCGATATGAAAATATTATAATATAGGTAGACAATTTTGAAGTTGGATAAAATTCTTATCATATATATGCCATTAAAACTATACTTGTTGTTCTATATAATAGCTTATAATATAAGTAAAAAGAAAATATATTGCTTAGACAATAAAGTGGTGTATATGCATGTTACTTCATTTTCAACAAATTAAGAAAATTATTCTACGTTTACAGTTTTAGTAAAACTTTGAACCAAAATCCAAAAATTATAATATTTAATTATTACATGTATTTCTATAAAGTACCAATAAGAAGTATAAGTCTTGATATTAATTAGAAGTATATACAAAGTGATCCAATAAAAACTTTCTATATATTTAGATAAATACTTTAATTAATGTGTTGCAAATTACAATATTAAAAATAAATTTTCATTTGAACCATTAAAATTTTAAACCATTTATGAATATGTACCTTGAATTCCATTAAGGTGTGACACAATTTCAATTGAATTATCAATCATTTTAATGACATTGACACTTGAATAAAAATTAACGTGACATTAAAAAAAAACATTTATGTGAGATATCATCTTTAAATGCTTAATATCAATACACCCTACCTTGATTTTAAAACTTAATTTTCTTTAACATTTTTTTTTTCCAAACTATAACAAATTTTTTTATGGAATTCTAAAGAAAAAGTTAGATATTTAGTATTTTAAAAATGGCAACTAAGAAAATGAAATGATAATAAATATGGATAATTAATTGGTGTAATTTGTTGGGGAAAAGGGTTTGGTTGTTGTCGATAATGAACGGAGACAAGACAGGGAGGCTATTGAAGTACGATCCACGTACCCAAAATGTCACCGTGTTGCGGAATGGCCTCGCTTTCCCCAACGGCGTGGCTTTGAACGCCGATTCTTCCTTCCTTCTAATGGCTGAAACCGGCACTCTCCAGGTCTTAAAGTTTTGGTTAAAGGTAATTATTAAATCATCATAACTTTTCTTGCTATACTTTTAAAAGTTTTACCTTTTCTTTTTTCTTTTTTTTCAGTTGTATCCATTTTTTTTAAATCTTAAATTTAGAAAAATATTGTGAATCTTATTTTATCTTAATTAATGATGCAAGGGTCCCAAAGCTAACACGATGGAGATATTCGCGCAACTTGAACGGTTTCCAGACAACATAAAAAGAACTGATAACGGTGACTTTTGGATAGCCATGAACAGTGCAAGAGGGACACTGGATACTCAAACGTGGAAGGAGCTGTACCGGGGAGCAACAATGAAGCAGGGGGAAGTGAAGATCCCGTGGATTCAGGCCGATCCGGTGGCGGTGAAGTTGAACGAGAGAGGGGAAGTGAAGGGGATGGTTGATGGAGGGGAGGGGCAAGCATTAGAGTCAGTGAGTGAGGTTGAAGAAAGTAGAGGGAGGTTGTGGATTGGTTCAGCTGTTAAACCATACGTTGGTTTAATCATCAATGGATAGAACCATAGAGTACTCAGTACACATTGGCATTTGAAAAAGAAAAGAAGGAGAAATGGATAAACTTCTGTGTTAAAATTATATTATTGTATCAAGTAAATTCTTGCAAAAGATTGTGTGTGTGTGTGTGTGTGTTTTTAGTTTGTAACAAAAACCCACCAACTGGTAAATTATGATGGGGGTATATATCTTCCCACGAGTTGTAAGAATTAAGATGGAATATATTTCATGGTTATCTGATTTGATATATATATCATGATGTTAACTTTCATGAAGT

Coding sequence (CDS)

ATGAAGAAGTGTGTGGGAGTTTGTTCATTGTTGGGTGCGGTGGCCGTTCTGTGTGTGGTGGCGGTGGTGGAAGGTGCAATTGAAGCCGTAGAGCTTCCCGGTGGTGTTTTCGGGCCGGAAAGTATAGCCTTTGACTGCCGTGGAGAAGGGCCCTACGCCAGTGTCTCCGATGGCAGAATCCTCAAGTGGAAAGGTCCTCACTTGGGTTGGACTCAATTTGCTCTTACCTCACCCAACAGGGAAGGGAAAGAATGCGATGGGCAGCCCCAAAGTGAGGCAGCATGTGGAAGGCCATTAGGGATCAAATTTCACCCTACAACTTGTGACCTTTACATTGCTGATGCTTATTTTGGCCTTCTGGCTGTAGGACCTAAGGGTGGCTTGGCTCGCCAGCTTGCCACTTCTGCTCAAGGCGTGCCACTTCGCTTCACTAATGCTCTTGATATTGATCCTCAAAATGGCATTGTTTATTTCACCGATAGTAGCATCCTCTTTCAAAGAAGGGTTTGGTTGTTGTCGATAATGAACGGAGACAAGACAGGGAGGCTATTGAAGTACGATCCACGTACCCAAAATGTCACCGTGTTGCGGAATGGCCTCGCTTTCCCCAACGGCGTGGCTTTGAACGCCGATTCTTCCTTCCTTCTAATGGCTGAAACCGGCACTCTCCAGGTCTTAAAGTTTTGGTTAAAGGGTCCCAAAGCTAACACGATGGAGATATTCGCGCAACTTGAACGGTTTCCAGACAACATAAAAAGAACTGATAACGGTGACTTTTGGATAGCCATGAACAGTGCAAGAGGGACACTGGATACTCAAACGTGGAAGGAGCTGTACCGGGGAGCAACAATGAAGCAGGGGGAAGTGAAGATCCCGTGGATTCAGGCCGATCCGGTGGCGGTGAAGTTGAACGAGAGAGGGGAAGTGAAGGGGATGGTTGATGGAGGGGAGGGGCAAGCATTAGAGTCAGTGAGTGAGGTTGAAGAAAGTAGAGGGAGGTTGTGGATTGGTTCAGCTGTTAAACCATACGTTGGTTTAATCATCAATGGATAG

Protein sequence

MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRILKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLLAVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKTGRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEIFAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELYRGATMKQGEVKIPWIQADPVAVKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING

Homology

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BLAST of Cucsat.G16310.T1 vs. ExPASy Swiss-Prot
Match: Q4V3D9 (Protein STRICTOSIDINE SYNTHASE-LIKE 10 OS=Arabidopsis thaliana OX=3702 GN=SSL10 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.1e-90
Identity = 165/319 (51.72%), Postives = 216/319 (67.71%), Query Frame = 0


Query: 35  GVFGPESIAFDCRGEGPYASVSDGRILKWKGPHLGWTQFALTSPNREGKECDGQPQSEAA 94
           G  GPESIAFD  GEGPY  VSDGRILKW+G  LGW+ FA TS NR+       P+ E  
Sbjct: 53  GASGPESIAFDPAGEGPYVGVSDGRILKWRGEPLGWSDFAHTSSNRQECARPFAPELEHV 112

Query: 95  CGRPLGIKFHPTTCDLYIADAYFGLLAVGPKGGLARQLATSAQGVPLRFTNALDIDPQNG 154
           CGRPLG++F   T DLYIADAYFGLL VGP GGLA+ L T A+G P RFTN LDID Q  
Sbjct: 113 CGRPLGLRFDKKTGDLYIADAYFGLLVVGPAGGLAKPLVTEAEGQPFRFTNDLDIDEQED 172

Query: 155 IVYFTDSSILFQRRVWLLSIMNGDKTGRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSF 214
           ++YFTD+S  FQRR +L +++N DKTGR +KYD  ++  TVL  GLAF NGVAL+ D SF
Sbjct: 173 VIYFTDTSARFQRRQFLAAVLNVDKTGRFIKYDRSSKKATVLLQGLAFANGVALSKDRSF 232

Query: 215 LLMAETGTLQVLKFWLKGPKANTMEIFAQLERFPDNIKRTDNGDFWIAMNSARGTLD--- 274
           +L+ ET T ++L+ WL GP A T ++FA+L  FPDNI+R  NG+FW+A++S +G      
Sbjct: 233 VLVVETTTCKILRLWLSGPNAGTHQVFAELPGFPDNIRRNSNGEFWVALHSKKGLFAKLS 292

Query: 275 -TQTW-KELYRGATMKQGEVKIPWIQADP--VAVKLNERGEVKGMVDGGEGQALESVSEV 334
            TQTW ++L     +    +   +    P   A+KL+E G+V  +++  EG+ L  +SEV
Sbjct: 293 LTQTWFRDLVLRLPISPQRLHSLFTGGIPHATAIKLSESGKVLEVLEDKEGKTLRFISEV 352

Query: 335 EESRGRLWIGSAVKPYVGL 347
           EE  G+LWIGS + P++G+
Sbjct: 353 EEKDGKLWIGSVLVPFLGV 371

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BLAST of Cucsat.G16310.T1 vs. ExPASy Swiss-Prot
Match: Q8VWF6 (Protein STRICTOSIDINE SYNTHASE-LIKE 3 OS=Arabidopsis thaliana OX=3702 GN=SSL3 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.4e-73
Identity = 150/330 (45.45%), Postives = 206/330 (62.42%), Query Frame = 0


Query: 36  VFGPESIAFDCRGEGPYASVSDGRILKWKGPHLGWTQFALTSPNREGKECDGQP------ 95
           V GPESIAFD +G GPY  V+DGRIL W G    WT FA TS NR  + CD +P      
Sbjct: 67  VQGPESIAFDPQGRGPYTGVADGRILFWNGTR--WTDFAYTSNNR-SELCDPKPSLLDYL 126

Query: 96  QSEAACGRPLGIKFHPTTCDLYIADAYFGLLAVGPKGGLARQLATSAQGVPLRFTNALDI 155
           + E  CGRPLG++F     DLYIADAY G++ VGP+GGLA  +   A GVPLRFTN LDI
Sbjct: 127 KDEDICGRPLGLRFDKKNGDLYIADAYLGIMKVGPEGGLATSVTNEADGVPLRFTNDLDI 186

Query: 156 DPQNGIVYFTDSSILFQRRVWLLSIMNGDKTGRLLKYDPRTQNVTVLRNGLAFPNGVALN 215
           D + G VYFTDSS  FQRR ++L I++G+ +GR+LKY+P+T+  T L   L FPNG++L 
Sbjct: 187 DDE-GNVYFTDSSSFFQRRKFMLLIVSGEDSGRVLKYNPKTKETTTLVRNLQFPNGLSLG 246

Query: 216 ADSSFLLMAETGTLQVLKFWLKGPKANTMEIFAQLERFPDNIKRTDNGDFWIAMNSARGT 275
            D SF +  E    ++ K+WLKG KA T E+ A L  FPDNI+   +GDFW+A++  R  
Sbjct: 247 KDGSFFIFCEGSIGRLRKYWLKGEKAGTSEVVALLHGFPDNIRTNKDGDFWVAVHCHR-N 306

Query: 276 LDTQTWKELYRGATMKQGEVKIP-------------WIQADPVAVKLNERGEVKGMVDGG 335
           + T       R   +++  +K+P             W  A  VAVK +E G+V  +++  
Sbjct: 307 IFTHLMAHYPR---VRKFFLKLPISVKFQYLLQVGGWPHA--VAVKYSEEGKVLKVLEDS 366

Query: 336 EGQALESVSEVEESRGRLWIGSAVKPYVGL 347
           +G+ +++VSEVEE  G+LW+GS +  ++ +
Sbjct: 367 KGKVVKAVSEVEEKDGKLWMGSVLMSFIAV 386

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BLAST of Cucsat.G16310.T1 vs. ExPASy Swiss-Prot
Match: Q9SLG8 (Protein STRICTOSIDINE SYNTHASE-LIKE 2 OS=Arabidopsis thaliana OX=3702 GN=SSL2 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 9.2e-70
Identity = 144/331 (43.50%), Postives = 198/331 (59.82%), Query Frame = 0


Query: 35  GVFGPESIAFDCRGEGPYASVSDGRILKWKGPHLGWTQFALTSPNREGKECDG---QPQS 94
           G  GPES  FD  G+GPY  +SDGRI+KW      W  FA+T+  REG  C+G     ++
Sbjct: 48  GALGPESFVFDFFGDGPYTGLSDGRIVKWLANESRWIDFAVTTSAREG--CEGPHEHQRT 107

Query: 95  EAACGRPLGIKFHPTTCDLYIADAYFGLLAVGPKGGLARQLATSAQGVPLRFTNALDIDP 154
           E  CGRPLG+ F  +T DLYIADAY GLL VGP GG+A Q+        LRFTN+LDI+P
Sbjct: 108 EHVCGRPLGLAFDKSTGDLYIADAYMGLLKVGPTGGVATQVLPRELNEALRFTNSLDINP 167

Query: 155 QNGIVYFTDSSILFQRRVWLLSIMNGDKTGRLLKYDPRTQNVTVLRNGLAFPNGVALNAD 214
           + G+VYFTDSS ++QRR ++ ++M+GDKTGRL+KYD  T+ VT L + LAF NGVAL+ +
Sbjct: 168 RTGVVYFTDSSSVYQRRNYIGAMMSGDKTGRLMKYD-NTKQVTTLLSNLAFVNGVALSQN 227

Query: 215 SSFLLMAETGTLQVLKFWL-----KGPKANTMEIFAQ-LERFPDNIKRTDNGDFWIAMNS 274
             +LL+ ET   ++L++WL     K    +  EIFA+ L  FPDNIKR+  G FW+ +N+
Sbjct: 228 GDYLLVVETAMCRILRYWLNETSVKSQSHDNYEIFAEGLPGFPDNIKRSPRGGFWVGLNT 287

Query: 275 ARGTLDTQTWKELYRGATMKQGEVKIPWIQADPV---------AVKLNE-RGEVKGMVDG 334
               L        + G       + + W++   V         AV+L+E  G +  + +G
Sbjct: 288 KHSKLTKFAMSNAWLGRAAL--GLPVDWMKVHSVWARYNGNGMAVRLSEDSGVILEVFEG 347

Query: 335 GEGQALESVSEVEESRGRLWIGSAVKPYVGL 347
                  S+SEVEE  G LW+GS   P+ G+
Sbjct: 348 KNENKWISISEVEEKDGTLWVGSVNTPFAGM 373

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BLAST of Cucsat.G16310.T1 vs. ExPASy Swiss-Prot
Match: Q9M1J6 (Protein STRICTOSIDINE SYNTHASE-LIKE 9 OS=Arabidopsis thaliana OX=3702 GN=SSL9 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.6e-68
Identity = 137/328 (41.77%), Postives = 199/328 (60.67%), Query Frame = 0


Query: 29  AVELPGGVFGPESIAFDCRGEGPYASVSDGRILKWKGPHLGWTQFALTSPNREGKECDGQ 88
           A  +P  V GPESI FD +GEGPYA+V DGRILKW+G  LGW  FA TSP+R    C  +
Sbjct: 44  AKTIPIPVAGPESIEFDPKGEGPYAAVVDGRILKWRGDDLGWVDFAYTSPHR--GNC-SK 103

Query: 89  PQSEAACGRPLGIKFHPTTCDLYIADAYFGLLAVGPKGGLARQLATSAQGVPLRFTNALD 148
            +    CGRPLG+ F   T DLYI D Y GL+ VGP+GGLA  +   A+G  + F N  D
Sbjct: 104 TEVVPTCGRPLGLTFEKKTGDLYICDGYLGLMKVGPEGGLAELIVDEAEGRKVMFANQGD 163

Query: 149 IDPQNGIVYFTDSSILFQRRVWLLSIMNGDKTGRLLKYDPRTQNVTVLRNGLAFPNGVAL 208
           ID +  + YF DSS  +  R      ++G+++GR+++YD +T+   V+ + L   NG+AL
Sbjct: 164 IDEEEDVFYFNDSSDKYHFRDVFFVAVSGERSGRVIRYDKKTKEAKVIMDNLVCNNGLAL 223

Query: 209 NADSSFLLMAETGTLQVLKFWLKGPKANTMEIFAQLERFPDNIKRTDNGDFWIAMNSARG 268
           N D SFL+  E+GT  V ++W+KGPKA T +IFA++  +PDNI+ T  GDFWI ++  + 
Sbjct: 224 NKDRSFLITCESGTSLVHRYWIKGPKAGTRDIFAKVPGYPDNIRLTSTGDFWIGLHCKKN 283

Query: 269 TLDTQTWKELYRGATMKQGEVKIPWIQA-------DPVAVKLN-ERGEVKGMVDGGEGQA 328
            +     K  + G  +++  +K+ ++ A         VAVK++ E GEV  +++  EG+ 
Sbjct: 284 LIGRLIVKYKWLGKLVEK-TMKLEYVIAFINGFKPHGVAVKISGETGEVLELLEDKEGKT 343

Query: 329 LESVSEV-EESRGRLWIGSAVKPYVGLI 348
           ++ VSE  E   G+LW GS   P V ++
Sbjct: 344 MKYVSEAYERDDGKLWFGSVYWPAVWVL 367

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BLAST of Cucsat.G16310.T1 vs. ExPASy Swiss-Prot
Match: F4IJZ6 (Protein STRICTOSIDINE SYNTHASE-LIKE 1 OS=Arabidopsis thaliana OX=3702 GN=SSL1 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.6e-66
Identity = 129/310 (41.61%), Postives = 186/310 (60.00%), Query Frame = 0


Query: 45  DCRGEGPYASVSDGRILKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFH 104
           D RGEGPY  V+DGRILKW G  LGW +FA +SP+R  K C    + E ACGRPLG+ F 
Sbjct: 85  DPRGEGPYVGVTDGRILKWSGEDLGWIEFAYSSPHR--KNCSSH-KVEPACGRPLGLSFE 144

Query: 105 PTTCDLYIADAYFGLLAVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSIL 164
             + DLY  D Y G++ VGPKGGLA ++    +G  + F N +DID +   +YF DSS  
Sbjct: 145 KKSGDLYFCDGYLGVMKVGPKGGLAEKVVDEVEGQKVMFANQMDIDEEEDAIYFNDSSDT 204

Query: 165 FQRRVWLLSIMNGDKTGRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQ 224
           +       + + G+KTGR ++YD +T+   V+ + L FPNG+AL+ D SF+L  E  T  
Sbjct: 205 YHFGDVFYAFLCGEKTGRAIRYDKKTKEAKVIMDRLHFPNGLALSIDGSFVLSCEVPTQL 264

Query: 225 VLKFWLKGPKANTMEIFAQLERFPDNIKRTDNGDFWIAMNSARGTLD----TQTWKELYR 284
           V ++W KGP A T +IFA+L  + DNI+RT+ GDFW+A++S +           W   + 
Sbjct: 265 VHRYWAKGPNAGTRDIFAKLPGYADNIRRTETGDFWVALHSKKTPFSRLSMIHPWVGKFF 324

Query: 285 GATMKQGEVKIPWIQADP--VAVKLN-ERGEVKGMVDGGEGQALESVSEVEESRGRLWIG 344
             T+K   +   +    P  VAVKL+ + GE+  +++  EG+ ++ +SEV+E  GRLW G
Sbjct: 325 IKTLKMELLVFLFEGGKPHAVAVKLSGKTGEIMEILEDSEGKNMKFISEVQERDGRLWFG 384

Query: 345 SAVKPYVGLI 348
           S   P V ++
Sbjct: 385 SVFLPSVWVL 391

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BLAST of Cucsat.G16310.T1 vs. NCBI nr
Match: XP_004150130.1 (protein STRICTOSIDINE SYNTHASE-LIKE 10 [Cucumis sativus] >KGN50870.1 hypothetical protein Csa_023098 [Cucumis sativus])

HSP 1 Score: 712 bits (1839), Expect = 3.26e-259
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 60
           MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI
Sbjct: 1   MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 60

Query: 61  LKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 120
           LKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL
Sbjct: 61  LKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 120

Query: 121 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKT 180
           AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKT
Sbjct: 121 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKT 180

Query: 181 GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEI 240
           GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEI
Sbjct: 181 GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEI 240

Query: 241 FAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELYRGATMKQGEVKIPWIQADPVA 300
           FAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELYRGATMKQGEVKIPWIQADPVA
Sbjct: 241 FAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELYRGATMKQGEVKIPWIQADPVA 300

Query: 301 VKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 350
           VKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING
Sbjct: 301 VKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 350

[Close]

BLAST of Cucsat.G16310.T1 vs. NCBI nr
Match: XP_008465977.1 (PREDICTED: protein STRICTOSIDINE SYNTHASE-LIKE 10-like [Cucumis melo])

HSP 1 Score: 689 bits (1779), Expect = 1.00e-249
Identity = 337/351 (96.01%), Postives = 346/351 (98.58%), Query Frame = 0


Query: 1   MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 60
           MKKCVGVCSLLGAVAVLC+V VVEGA+EAVELPGGVFGPESIAFDCRGEGPYASVSDGRI
Sbjct: 21  MKKCVGVCSLLGAVAVLCMVGVVEGAVEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 80

Query: 61  LKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 120
           LKWKGP LGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL
Sbjct: 81  LKWKGPDLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 140

Query: 121 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKT 180
           AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNG+VYFTDSSILFQRRVWLLSIMNGDKT
Sbjct: 141 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGVVYFTDSSILFQRRVWLLSIMNGDKT 200

Query: 181 GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEI 240
           GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQ+LKFWLKGPKANTMEI
Sbjct: 201 GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQILKFWLKGPKANTMEI 260

Query: 241 FAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELY-RGATMKQGEVKIPWIQADPV 300
           FAQLERFPDNIKRTDNGDFWIAMN+ARG LDTQTWKE+Y RGA ++QGEVKIPWIQADPV
Sbjct: 261 FAQLERFPDNIKRTDNGDFWIAMNTARGKLDTQTWKEMYMRGAKLQQGEVKIPWIQADPV 320

Query: 301 AVKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 350
           AVKL+ERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING
Sbjct: 321 AVKLDERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 371

[Close]

BLAST of Cucsat.G16310.T1 vs. NCBI nr
Match: KAA0050238.1 (protein STRICTOSIDINE SYNTHASE-LIKE 10-like [Cucumis melo var. makuwa] >TYJ98168.1 protein STRICTOSIDINE SYNTHASE-LIKE 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 687 bits (1773), Expect = 4.53e-249
Identity = 337/351 (96.01%), Postives = 345/351 (98.29%), Query Frame = 0


Query: 1   MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 60
           MKKCVGVCSLLGAVAVLC+V VVEGA+EAVELPGGVFGPESIAFDCRGEGPYASVSDGRI
Sbjct: 5   MKKCVGVCSLLGAVAVLCMVGVVEGAVEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 64

Query: 61  LKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 120
           LKWKGP LGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL
Sbjct: 65  LKWKGPDLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 124

Query: 121 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKT 180
           AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNG+VYFTDSSILFQRRVWLLSIMNGDKT
Sbjct: 125 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGVVYFTDSSILFQRRVWLLSIMNGDKT 184

Query: 181 GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEI 240
           GRLLKYDPRTQNVTVLR GLAFPNGVALNADSSFLLMAETGTLQ+LKFWLKGPKANTMEI
Sbjct: 185 GRLLKYDPRTQNVTVLRYGLAFPNGVALNADSSFLLMAETGTLQILKFWLKGPKANTMEI 244

Query: 241 FAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELY-RGATMKQGEVKIPWIQADPV 300
           FAQLERFPDNIKRTDNGDFWIAMN+ARG LDTQTWKELY RGA ++QGEVKIPWIQADPV
Sbjct: 245 FAQLERFPDNIKRTDNGDFWIAMNTARGKLDTQTWKELYMRGAKLQQGEVKIPWIQADPV 304

Query: 301 AVKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 350
           AVKL+ERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING
Sbjct: 305 AVKLDERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 355

[Close]

BLAST of Cucsat.G16310.T1 vs. NCBI nr
Match: XP_023536583.1 (protein STRICTOSIDINE SYNTHASE-LIKE 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 579 bits (1493), Expect = 2.16e-206
Identity = 282/357 (78.99%), Postives = 315/357 (88.24%), Query Frame = 0


Query: 1   MKKCVGVCSLLGAVAVLCVVAVV-------EGAIEAVELPGGVFGPESIAFDCRGEGPYA 60
           MK+ + +CS   ++ +  VVA V       E AIEAVELPGGVFGPESIAFDCRGEGPYA
Sbjct: 1   MKESLAICSCSLSLVLWVVVAAVVSWSPLSEAAIEAVELPGGVFGPESIAFDCRGEGPYA 60

Query: 61  SVSDGRILKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIA 120
            V DGRILKW G  LGWTQFA TSPNREGKEC+G+PQ+EA CGRPLG+KFHP+TC+L+IA
Sbjct: 61  GVGDGRILKWNGSDLGWTQFAYTSPNREGKECNGEPQTEAGCGRPLGLKFHPSTCELFIA 120

Query: 121 DAYFGLLAVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLS 180
           DAYFGLLAVGP+GGLARQL +SAQGVPLRFTNALDIDPQNG+VYFTDSS+LFQR VW+LS
Sbjct: 121 DAYFGLLAVGPQGGLARQLVSSAQGVPLRFTNALDIDPQNGVVYFTDSSLLFQRSVWILS 180

Query: 181 IMNGDKTGRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGP 240
           I+NGD+TGRLLKYDPRT+NVT+L NGLAFPNGVAL+ DSSFLLMAETGTLQ+LKFWLKGP
Sbjct: 181 IVNGDRTGRLLKYDPRTKNVTILINGLAFPNGVALSTDSSFLLMAETGTLQILKFWLKGP 240

Query: 241 KANTMEIFAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELYRGATMKQGEVKIPW 300
           KA T EIFAQLERFPDNIKRTDNG+FWIAMN+ RG L+TQTW  L  GAT + GEVKIPW
Sbjct: 241 KAQTTEIFAQLERFPDNIKRTDNGEFWIAMNTGRGKLETQTWMGL-GGATTQHGEVKIPW 300

Query: 301 IQADPVAVKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 350
           I+ADPVAVK++ERG VKGM+DG EGQALESVSEVEE +GRLWIGSAVKPYVG IING
Sbjct: 301 IRADPVAVKVDERGGVKGMIDGEEGQALESVSEVEERKGRLWIGSAVKPYVGFIING 356

[Close]

BLAST of Cucsat.G16310.T1 vs. NCBI nr
Match: XP_022961558.1 (protein STRICTOSIDINE SYNTHASE-LIKE 10-like [Cucurbita moschata])

HSP 1 Score: 577 bits (1488), Expect = 1.25e-205
Identity = 275/327 (84.10%), Postives = 300/327 (91.74%), Query Frame = 0


Query: 24  EGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRILKWKGPHLGWTQFALTSPNREGK 83
           E AIEAVELPGGVFGPESIAFDCRGEGPYA V DGRILKW G  LGWTQFA TSPNREGK
Sbjct: 31  EAAIEAVELPGGVFGPESIAFDCRGEGPYAGVGDGRILKWNGSDLGWTQFAYTSPNREGK 90

Query: 84  ECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLLAVGPKGGLARQLATSAQGVPLRF 143
           EC+G+PQ+EA CGRPLG+KFHP+TC+L+IADAYFGLLAVGP+GGLARQL +SAQGVPLRF
Sbjct: 91  ECNGEPQTEAGCGRPLGLKFHPSTCELFIADAYFGLLAVGPQGGLARQLVSSAQGVPLRF 150

Query: 144 TNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKTGRLLKYDPRTQNVTVLRNGLAFP 203
           TNALDIDPQNG+VYFTDSS+LFQR VW+LSI+NGD+TGRLLKYDP T+NVTVL NGLAFP
Sbjct: 151 TNALDIDPQNGVVYFTDSSLLFQRSVWILSIVNGDRTGRLLKYDPCTKNVTVLINGLAFP 210

Query: 204 NGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEIFAQLERFPDNIKRTDNGDFWIAM 263
           NGVAL+ DSSFLLMAETGTLQ+LKFWLKGPKA T EIFAQLERFPDNIKRTDNG+FWIAM
Sbjct: 211 NGVALSTDSSFLLMAETGTLQILKFWLKGPKAQTTEIFAQLERFPDNIKRTDNGEFWIAM 270

Query: 264 NSARGTLDTQTWKELYRGATMKQGEVKIPWIQADPVAVKLNERGEVKGMVDGGEGQALES 323
           N+ RG L+TQTW  L  GAT + GEVKIPWI+ DPVAVKL+ERGEVKGM+DG EGQALES
Sbjct: 271 NTGRGKLETQTWMGL-GGATTQHGEVKIPWIRGDPVAVKLDERGEVKGMIDGEEGQALES 330

Query: 324 VSEVEESRGRLWIGSAVKPYVGLIING 350
           VSEVEE +GRLWIGSAVKPYVG IING
Sbjct: 331 VSEVEERKGRLWIGSAVKPYVGFIING 356

[Close]

BLAST of Cucsat.G16310.T1 vs. ExPASy TrEMBL
Match: A0A0A0KMJ0 (Str_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G305760 PE=3 SV=1)

HSP 1 Score: 712 bits (1839), Expect = 1.58e-259
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 60
           MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI
Sbjct: 1   MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 60

Query: 61  LKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 120
           LKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL
Sbjct: 61  LKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 120

Query: 121 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKT 180
           AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKT
Sbjct: 121 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKT 180

Query: 181 GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEI 240
           GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEI
Sbjct: 181 GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEI 240

Query: 241 FAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELYRGATMKQGEVKIPWIQADPVA 300
           FAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELYRGATMKQGEVKIPWIQADPVA
Sbjct: 241 FAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELYRGATMKQGEVKIPWIQADPVA 300

Query: 301 VKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 350
           VKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING
Sbjct: 301 VKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 350

[Close]

BLAST of Cucsat.G16310.T1 vs. ExPASy TrEMBL
Match: A0A1S3CQG7 (protein STRICTOSIDINE SYNTHASE-LIKE 10-like OS=Cucumis melo OX=3656 GN=LOC103503543 PE=3 SV=1)

HSP 1 Score: 689 bits (1779), Expect = 4.87e-250
Identity = 337/351 (96.01%), Postives = 346/351 (98.58%), Query Frame = 0


Query: 1   MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 60
           MKKCVGVCSLLGAVAVLC+V VVEGA+EAVELPGGVFGPESIAFDCRGEGPYASVSDGRI
Sbjct: 21  MKKCVGVCSLLGAVAVLCMVGVVEGAVEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 80

Query: 61  LKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 120
           LKWKGP LGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL
Sbjct: 81  LKWKGPDLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 140

Query: 121 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKT 180
           AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNG+VYFTDSSILFQRRVWLLSIMNGDKT
Sbjct: 141 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGVVYFTDSSILFQRRVWLLSIMNGDKT 200

Query: 181 GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEI 240
           GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQ+LKFWLKGPKANTMEI
Sbjct: 201 GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQILKFWLKGPKANTMEI 260

Query: 241 FAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELY-RGATMKQGEVKIPWIQADPV 300
           FAQLERFPDNIKRTDNGDFWIAMN+ARG LDTQTWKE+Y RGA ++QGEVKIPWIQADPV
Sbjct: 261 FAQLERFPDNIKRTDNGDFWIAMNTARGKLDTQTWKEMYMRGAKLQQGEVKIPWIQADPV 320

Query: 301 AVKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 350
           AVKL+ERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING
Sbjct: 321 AVKLDERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 371

[Close]

BLAST of Cucsat.G16310.T1 vs. ExPASy TrEMBL
Match: A0A5A7U4P2 (Protein STRICTOSIDINE SYNTHASE-LIKE 10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00510 PE=3 SV=1)

HSP 1 Score: 687 bits (1773), Expect = 2.19e-249
Identity = 337/351 (96.01%), Postives = 345/351 (98.29%), Query Frame = 0


Query: 1   MKKCVGVCSLLGAVAVLCVVAVVEGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 60
           MKKCVGVCSLLGAVAVLC+V VVEGA+EAVELPGGVFGPESIAFDCRGEGPYASVSDGRI
Sbjct: 5   MKKCVGVCSLLGAVAVLCMVGVVEGAVEAVELPGGVFGPESIAFDCRGEGPYASVSDGRI 64

Query: 61  LKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 120
           LKWKGP LGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL
Sbjct: 65  LKWKGPDLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLL 124

Query: 121 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKT 180
           AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNG+VYFTDSSILFQRRVWLLSIMNGDKT
Sbjct: 125 AVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGVVYFTDSSILFQRRVWLLSIMNGDKT 184

Query: 181 GRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEI 240
           GRLLKYDPRTQNVTVLR GLAFPNGVALNADSSFLLMAETGTLQ+LKFWLKGPKANTMEI
Sbjct: 185 GRLLKYDPRTQNVTVLRYGLAFPNGVALNADSSFLLMAETGTLQILKFWLKGPKANTMEI 244

Query: 241 FAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELY-RGATMKQGEVKIPWIQADPV 300
           FAQLERFPDNIKRTDNGDFWIAMN+ARG LDTQTWKELY RGA ++QGEVKIPWIQADPV
Sbjct: 245 FAQLERFPDNIKRTDNGDFWIAMNTARGKLDTQTWKELYMRGAKLQQGEVKIPWIQADPV 304

Query: 301 AVKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 350
           AVKL+ERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING
Sbjct: 305 AVKLDERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 355

[Close]

BLAST of Cucsat.G16310.T1 vs. ExPASy TrEMBL
Match: A0A6J1HC56 (protein STRICTOSIDINE SYNTHASE-LIKE 10-like OS=Cucurbita moschata OX=3662 GN=LOC111462102 PE=3 SV=1)

HSP 1 Score: 577 bits (1488), Expect = 6.04e-206
Identity = 275/327 (84.10%), Postives = 300/327 (91.74%), Query Frame = 0


Query: 24  EGAIEAVELPGGVFGPESIAFDCRGEGPYASVSDGRILKWKGPHLGWTQFALTSPNREGK 83
           E AIEAVELPGGVFGPESIAFDCRGEGPYA V DGRILKW G  LGWTQFA TSPNREGK
Sbjct: 31  EAAIEAVELPGGVFGPESIAFDCRGEGPYAGVGDGRILKWNGSDLGWTQFAYTSPNREGK 90

Query: 84  ECDGQPQSEAACGRPLGIKFHPTTCDLYIADAYFGLLAVGPKGGLARQLATSAQGVPLRF 143
           EC+G+PQ+EA CGRPLG+KFHP+TC+L+IADAYFGLLAVGP+GGLARQL +SAQGVPLRF
Sbjct: 91  ECNGEPQTEAGCGRPLGLKFHPSTCELFIADAYFGLLAVGPQGGLARQLVSSAQGVPLRF 150

Query: 144 TNALDIDPQNGIVYFTDSSILFQRRVWLLSIMNGDKTGRLLKYDPRTQNVTVLRNGLAFP 203
           TNALDIDPQNG+VYFTDSS+LFQR VW+LSI+NGD+TGRLLKYDP T+NVTVL NGLAFP
Sbjct: 151 TNALDIDPQNGVVYFTDSSLLFQRSVWILSIVNGDRTGRLLKYDPCTKNVTVLINGLAFP 210

Query: 204 NGVALNADSSFLLMAETGTLQVLKFWLKGPKANTMEIFAQLERFPDNIKRTDNGDFWIAM 263
           NGVAL+ DSSFLLMAETGTLQ+LKFWLKGPKA T EIFAQLERFPDNIKRTDNG+FWIAM
Sbjct: 211 NGVALSTDSSFLLMAETGTLQILKFWLKGPKAQTTEIFAQLERFPDNIKRTDNGEFWIAM 270

Query: 264 NSARGTLDTQTWKELYRGATMKQGEVKIPWIQADPVAVKLNERGEVKGMVDGGEGQALES 323
           N+ RG L+TQTW  L  GAT + GEVKIPWI+ DPVAVKL+ERGEVKGM+DG EGQALES
Sbjct: 271 NTGRGKLETQTWMGL-GGATTQHGEVKIPWIRGDPVAVKLDERGEVKGMIDGEEGQALES 330

Query: 324 VSEVEESRGRLWIGSAVKPYVGLIING 350
           VSEVEE +GRLWIGSAVKPYVG IING
Sbjct: 331 VSEVEERKGRLWIGSAVKPYVGFIING 356

[Close]

BLAST of Cucsat.G16310.T1 vs. ExPASy TrEMBL
Match: A0A6J1JQT3 (protein STRICTOSIDINE SYNTHASE-LIKE 10-like OS=Cucurbita maxima OX=3661 GN=LOC111486992 PE=3 SV=1)

HSP 1 Score: 575 bits (1482), Expect = 4.96e-205
Identity = 280/357 (78.43%), Postives = 313/357 (87.68%), Query Frame = 0


Query: 1   MKKCVGVCSLLGAVAVLCVVAVV-------EGAIEAVELPGGVFGPESIAFDCRGEGPYA 60
           MK+ + +CS   ++A+  V  VV       E AIEAVELPGGVFGPESIAFDCRGEGPYA
Sbjct: 1   MKESLAICSCSLSLALWVVAVVVVSWSPLSEAAIEAVELPGGVFGPESIAFDCRGEGPYA 60

Query: 61  SVSDGRILKWKGPHLGWTQFALTSPNREGKECDGQPQSEAACGRPLGIKFHPTTCDLYIA 120
            V DGRILKW G  LGWTQFA TSPNREGKEC+G+PQ+EAACGRPLG+KFHP+TC+L+IA
Sbjct: 61  GVGDGRILKWNGSGLGWTQFAYTSPNREGKECNGEPQTEAACGRPLGLKFHPSTCELFIA 120

Query: 121 DAYFGLLAVGPKGGLARQLATSAQGVPLRFTNALDIDPQNGIVYFTDSSILFQRRVWLLS 180
           DAYFGLLAVGP+GGLA QL +SAQGVPLRFTNALDIDPQNG+VYFTDSS+LFQR VW+LS
Sbjct: 121 DAYFGLLAVGPQGGLASQLVSSAQGVPLRFTNALDIDPQNGVVYFTDSSLLFQRSVWILS 180

Query: 181 IMNGDKTGRLLKYDPRTQNVTVLRNGLAFPNGVALNADSSFLLMAETGTLQVLKFWLKGP 240
           I+NGD+TGRLLKYDPRT+NVTVL NGLAFPNGVAL+ DSSFLLMAETGTLQ+LKFWLKGP
Sbjct: 181 IVNGDRTGRLLKYDPRTKNVTVLINGLAFPNGVALSTDSSFLLMAETGTLQILKFWLKGP 240

Query: 241 KANTMEIFAQLERFPDNIKRTDNGDFWIAMNSARGTLDTQTWKELYRGATMKQGEVKIPW 300
           KA T EIF QLERFPDNIKRTDNG+FWIAMN+ RG L+TQTW  L  GAT + GEVKIPW
Sbjct: 241 KAQTTEIFVQLERFPDNIKRTDNGEFWIAMNTGRGKLETQTWMRL-GGATTQHGEVKIPW 300

Query: 301 IQADPVAVKLNERGEVKGMVDGGEGQALESVSEVEESRGRLWIGSAVKPYVGLIING 350
           I+ DPVAVKL+ERG VKGM+DG EGQALESVSEV+E +GRLWIGSAVKPYVG I+NG
Sbjct: 301 IRGDPVAVKLDERGGVKGMIDGEEGQALESVSEVDERKGRLWIGSAVKPYVGFIMNG 356

The following BLAST results are available for this feature:

BLAST of Cucsat.G16310.T1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4V3D9	2.1e-90	51.72	Protein STRICTOSIDINE SYNTHASE-LIKE 10 OS=Arabidopsis thaliana OX=3702 GN=SSL10 ...	[more]
Q8VWF6	1.4e-73	45.45	Protein STRICTOSIDINE SYNTHASE-LIKE 3 OS=Arabidopsis thaliana OX=3702 GN=SSL3 PE...	[more]
Q9SLG8	9.2e-70	43.50	Protein STRICTOSIDINE SYNTHASE-LIKE 2 OS=Arabidopsis thaliana OX=3702 GN=SSL2 PE...	[more]
Q9M1J6	6.6e-68	41.77	Protein STRICTOSIDINE SYNTHASE-LIKE 9 OS=Arabidopsis thaliana OX=3702 GN=SSL9 PE...	[more]
F4IJZ6	1.6e-66	41.61	Protein STRICTOSIDINE SYNTHASE-LIKE 1 OS=Arabidopsis thaliana OX=3702 GN=SSL1 PE...	[more]

BLAST of Cucsat.G16310.T1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150130.1	3.26e-259	100.00	protein STRICTOSIDINE SYNTHASE-LIKE 10 [Cucumis sativus] >KGN50870.1 hypothetica...	[more]
XP_008465977.1	1.00e-249	96.01	PREDICTED: protein STRICTOSIDINE SYNTHASE-LIKE 10-like [Cucumis melo]	[more]
KAA0050238.1	4.53e-249	96.01	protein STRICTOSIDINE SYNTHASE-LIKE 10-like [Cucumis melo var. makuwa] >TYJ98168...	[more]
XP_023536583.1	2.16e-206	78.99	protein STRICTOSIDINE SYNTHASE-LIKE 10-like [Cucurbita pepo subsp. pepo]	[more]
XP_022961558.1	1.25e-205	84.10	protein STRICTOSIDINE SYNTHASE-LIKE 10-like [Cucurbita moschata]	[more]

BLAST of Cucsat.G16310.T1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KMJ0	1.58e-259	100.00	Str_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G305760 P...	[more]
A0A1S3CQG7	4.87e-250	96.01	protein STRICTOSIDINE SYNTHASE-LIKE 10-like OS=Cucumis melo OX=3656 GN=LOC103503...	[more]
A0A5A7U4P2	2.19e-249	96.01	Protein STRICTOSIDINE SYNTHASE-LIKE 10-like OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A6J1HC56	6.04e-206	84.10	protein STRICTOSIDINE SYNTHASE-LIKE 10-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1JQT3	4.96e-205	78.43	protein STRICTOSIDINE SYNTHASE-LIKE 10-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011042	Six-bladed beta-propeller, TolB-like	GENE3D	2.120.10.30		coord: 20..350 e-value: 9.7E-126 score: 420.9
IPR018119	Strictosidine synthase, conserved region	PFAM	PF03088	Str_synth	coord: 145..233 e-value: 7.1E-28 score: 96.6
None	No IPR available	PANTHER	PTHR10426:SF86	OS09G0374900 PROTEIN	coord: 24..347
None	No IPR available	PANTHER	PTHR10426	STRICTOSIDINE SYNTHASE-RELATED	coord: 24..347
None	No IPR available	SUPERFAMILY	63829	Calcium-dependent phosphotriesterase	coord: 29..342

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G16310	Cucsat.G16310	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G16310.T1.E1	Cucsat.G16310.T1.E1	exon
Cucsat.G16310.T1.E2	Cucsat.G16310.T1.E2	exon
Cucsat.G16310.T1.E3	Cucsat.G16310.T1.E3	exon
Cucsat.G16310.T1.E4	Cucsat.G16310.T1.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G16310.T1.C4	Cucsat.G16310.T1.C4	CDS
Cucsat.G16310.T1.C3	Cucsat.G16310.T1.C3	CDS
Cucsat.G16310.T1.C2	Cucsat.G16310.T1.C2	CDS
Cucsat.G16310.T1.C1	Cucsat.G16310.T1.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G16310.T1	Cucsat.G16310.T1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009058	biosynthetic process
cellular_component	GO:0005773	vacuole
molecular_function	GO:0016844	strictosidine synthase activity