Cucsat.G13250.T5 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G13250.T5
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	aquaporin NIP2-1-like
Location	ctg1838: 3148812 .. 3152354 (+)
RNA-Seq Expression	Cucsat.G13250.T5
Synteny	Cucsat.G13250.T5

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTATTTTCTAAGTTAGCTCAAAAAAAAAAAAAAAATAGTTACGTAAATTTCTCTCCCTATAATTCCTTGGCATTTCCTTATCAGAAATAAAAGAAATAGGAAGACAATAGGAAATTCACATTTTTGTTGCAAAAAGGCAAAGTGGCTAATTCTTATCTTTTAACAAAAATACCCCCAACAAATTTATTTATATGAAAATGAAAACCCAAAACAATATTCAAACCCTAAAACGAAAAAATAACATTTAATAGTATTTATTTTAATTTTTCGATTGGTCCCCAGCCACACTACACACTATACCGATTAATTTCGTTGCCTATAAGATATTTCCTTCCCTTCCCTTCCTTCAAAAATAAAACCATCTTACCAAAAATATTCAAACAAATAAATCACTCCCAATTTTTCAAATTCCCTAAAAAAAAAATGGCGAGACGCAGCGACGATGAAGAAGCCTTTATTGCCTTGGGAAACGAGTGCTCTGATCCTCAACCACCCTTGTTCCGCGACCGATTTGATGAACTTTACCCGCCCGAGTTCTCACGAAAGGTATGGATTTCTAGTTAATTTTGTTCTTGGTTGAATTTGAAGCGATTGGTTTGTGATCGGAGGAATTTTTGTGGTGGTTTAGCTTGTGGCGGAGGTGATCGCTACGTATTTGCTTGTGTTTGTGTCGTGTGGTGTGGCGGCATTGAGTGGGAGTGATGAACCGGTAGTGACGAAGCTTGGAGCTTCAATTACCTGTGGATTGATTGTGACGGTGATGATTTACTCCGTCGGACATATCTCCGGCGCTCATATGAATCCAGCGGTTACTATTGCTTTTGCTGCTGTTCGGCGATTTCCATGGAGACAGGTCATCTTCTTCTTCCTCCTCTGTTTTTCTCCCCAATTTTTATGCCAATTTACCATATTATCCTTTCGCTTTTTCATTTGTTATTTTTATGTTTTCTACAGATATTTTCTATTTTGAGAAAAGCCGTGCCAAATATTTAATTCACTTTTTTGCCTTTTTTTTTCTTCTTTTTTTAAGTTTTGATATTTAGTTTAAGAGTGGAAACCTATACTTAAAAAAGTTTCGAAATATTACATTTGTACCATTTAATTTTTTTATCATGTTATTTATTTTTAATTCTTTATTTTAAATTTAGTTTTCATTTACTCCCTACATTTCAATATTTAGTTTCAATTTTTGTTTGTTAATAGCACTGCTTGTTTTGTTATTCATACTTCAATAATTAAGTTCAAATGATTCTATCATAATTTTTTTACTTATAATTAATTAATTAATTTTTAGATATTAAAATTGATGTTGAGTATTTAAACCATATAAAATTCAAAAATCATGCTACATTTTCATGACTTAAAATACAGGAATTAAAAAGTTCCTTTTTTTTTTCTAAATGTAAAAAGAAAAAAAAAAGTTTTTCTTTCTTTCTTTTCAACATTAAAAGCATGCTTAGATATTGGTGGTTGAGTTGATAAGTGTACTGAGAATAGATGTGTATTAGGTAATATAATAAAAATGTCCAATAAAAAGACAAAACAATGTCTCCTTTCTTTTAACATTTAGAAAACAAAATCAAATTTATCATTATTTTTTTCAAGTTTACTAAATTAAAAATAGTAGAATTCTTATTTTAACTTTGGTACAAAATATTTGAACAATTATACACCGACAAATAATCACCCTGAATCTAGAGCTTTGGATTTCTAGTGCACTCTCTCATTTTCGAAAAATAAAATAAAATTATCAACATATTCTCTCTCTCTTTTTTTTTTTTCTTTTCAGAATACATACTCTTTTTTGCTCTATTTCACTCTTTTTTAAAAAATTTAAAAATTAAGAATATATACTCTTTTTCTTTTTCTTTTTTAGATTATATACTATCTTAATCTCTCTTTTTTTTCTGAAACTGTATTAAAAATTAAGATTATATATACTCTCTTACTTTTTTTTTTATTTAAGAATTATAATCTCTTACTTAAGAATTAAGAAAAAGGAACCCCCAATCAAATCAACAGTGATGGTGATATATATTATAAATGTCTATTTTATTGGACGGGCGGAAATTAATTATAATTTTTTTAAAATTGGAAATTTAGTGGTAATGATGTATGAGCTGCGCCACTTCATCAAAAAGAACAGTAAAAAATTATAAAAAAAGGGATGGAAAAGTGCAATTTATGCCACCTGAGAAGTGACGTGGATGAAGTGTACGGAAGATATTTCGATAAGTGTCCCCACCAGTTTGGCAGATATTATATATAAAAAGACTATTGGCAGATACTTTCTAAGGAAAGCAGCCTTAATTGCTTTTTAATTCACCAAAAGTCATCTTAATTGAGAATATGCTTTTCCATCTTTTCCAGTTTAGACCTTTCAAACCTTTTTTCTTTTTAACTCTTTTTCTATTTTATTTTCATATATATTTTTTACATGACTTAGTAATAATTGATGTATACTCTTATGGTTCGTGAATTGAATTTTATGCGGTTGATGCAGGTTCCACTTTATGCAGCAGCTCAATTAAGTGGAGCTACGTCTGCAGCCTTCACACTACGCATATTAATGGATCCAATTCAAGATTTAGGTACTACTTCACCACATGGACCGGCTTTGAAAGCACTTGTTATGGAGATTGTTGTCTCATTTTGTATGATGTTTGTCACTTCTGCGGTTGCCACCGACACCAAAGCTGTAAGAGCATCTTATTGTTGTTTACCTCTTACTCACAATCTTCTCTTAATTTCCTTTCGCTAAGGTTCACTGCTTTTTTATATGTAATTTTTGGCAGATAGGAGAGCTTGGAGGCGTAGCTGTGGGGTCAGCTGTATGTATCTCGTCCATCTTTGCTGGGTAAGCAAAGCAATTATAATTGTTTCTTTTTTTGGCTTTTTGTTCTTAGCTGGAAGTTTTGTGGGTATCTGGAAAAATCTTAAACAAGATTTTGTTTTGATTGAATTCCAAATTTGGACCCTATAGTTTGGAGAAAATTTGTTTATTTATGAGATTTTATCAAACTATAAAGCTGGGGTTAACTATGTGTAGGCCAATATCGGGTGGATCGATGAACCCAGCAAGATCAATAGGACCAGCCATTGCAAGTTCACGTTACGAAGGAATTTGGGTGTACATGATCGGTCCAGTTACAGGAACCTTGCTTGCATCATTTTCATATAATTTCATACGAGCCACTGAAAAACACACCCATTCACTCTCGTTACATTGACATCCAATCGATGGGATCATGCAGCTGGAACTTCAAGTCATACCAAAATCAACTAGTTCAATTATTGTATAAATTAATGTGTAGCTCCTATAATAAAAGTTGTAGTTTAAGTTGTAGCTTTAGAAAAACTTAAGGTAAGGTTAGCTTTAATTGTCTTGCAATTTATTTGTTTTTTTAGTTTCGAGAGAAGAATGAATCTGAAATAGAGATTCATTTTTCTTTTTATTACTCTTGTTATTTTACAAGTGTGAGTTTTGATCTGATTTTTTTTACAATAATCAATCATAAAATTGGTTCTTCTTTTCAAGTTA

Coding sequence (CDS)

ATGGCGAGACGCAGCGACGATGAAGAAGCCTTTATTGCCTTGGGAAACGAGTGCTCTGATCCTCAACCACCCTTGTTCCGCGACCGATTTGATGAACTTTACCCGCCCGAGTTCTCACGAAAGCTTGTGGCGGAGGTGATCGCTACGTATTTGCTTGTGTTTGTGTCGTGTGGTGTGGCGGCATTGAGTGGGAGTGATGAACCGGTAGTGACGAAGCTTGGAGCTTCAATTACCTGTGGATTGATTGTGACGGTGATGATTTACTCCGTCGGACATATCTCCGGCGCTCATATGAATCCAGCGGTTACTATTGCTTTTGCTGCTGTTCGGCGATTTCCATGGAGACAGGTCATCTTCTTCTTCCTCCTCTGTTTTTCTCCCCAATTTTTATGCCAATTTACCATATTATCCTTTCGCTTTTTCATTTGTTATTTTTATGTTTTCTACAGATATTTTCTATTTTGA

Protein sequence

MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVIFFFLLCFSPQFLCQFTILSFRFFICYFYVFYRYFLF

Homology

[Close]

BLAST of Cucsat.G13250.T5 vs. ExPASy Swiss-Prot
Match: Q19KC1 (Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 4.2e-27
Identity = 61/123 (49.59%), Postives = 86/123 (69.92%), Query Frame = 0


Query: 14  LGNECSDPQPPLFRDR-FDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTK 73
           +G   +   PP + DR   +++PP   +K+V+EV++T+LLVFV+CG A + GSD+  +++
Sbjct: 20  IGTAQNSSMPPTYYDRSLADIFPPHLLKKVVSEVVSTFLLVFVTCGAAGIYGSDKDRISQ 79

Query: 74  LGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVIFFFLLCFSPQFLCQ 133
           LG S+  GLIVTVMIY+VGHISGAHMNPAVT+AFA  R FPW QV F++   F+      
Sbjct: 80  LGQSVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGSICAS 139

Query: 134 FTI 136
           F +
Sbjct: 140 FVL 142

[Close]

BLAST of Cucsat.G13250.T5 vs. ExPASy Swiss-Prot
Match: Q6Z2T3 (Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.0e-25
Identity = 58/104 (55.77%), Postives = 77/104 (74.04%), Query Frame = 0


Query: 32  ELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTKLGASITCGLIVTVMIYSVG 91
           + +PP   +K+V+EV+AT+LLVF++CG A +SGSD   +++LG SI  GLIVTVMIY+VG
Sbjct: 41  DFFPPHLLKKVVSEVVATFLLVFMTCGAAGISGSDLSRISQLGQSIAGGLIVTVMIYAVG 100

Query: 92  HISGAHMNPAVTIAFAAVRRFPWRQVIFFFLLCFSPQFLCQFTI 136
           HISGAHMNPAVT+AFA  R FPW QV F++   F+      F +
Sbjct: 101 HISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGAICASFVL 144

[Close]

BLAST of Cucsat.G13250.T5 vs. ExPASy Swiss-Prot
Match: Q9AT74 (Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.4e-24
Identity = 53/104 (50.96%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 32  ELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTKLGASITCGLIVTVMIYSVG 91
           +++PP   +K+++EV+AT+LLVFV+CG A++ G D   +++LG S+  GLIVTVMIY+ G
Sbjct: 47  DIFPPHLGKKVISEVVATFLLVFVTCGAASIYGEDNARISQLGQSVAGGLIVTVMIYATG 106

Query: 92  HISGAHMNPAVTIAFAAVRRFPWRQVIFFFLLCFSPQFLCQFTI 136
           HISGAHMNPAVT++FA  R FPW QV F++   F+      F +
Sbjct: 107 HISGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVL 150

[Close]

BLAST of Cucsat.G13250.T5 vs. ExPASy Swiss-Prot
Match: Q9ATN2 (Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 4.4e-24
Identity = 53/104 (50.96%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 32  ELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTKLGASITCGLIVTVMIYSVG 91
           +++PP   +K+++EV+AT+LLVFV+CG A++ G D   +++LG S+  GLIVTVMIY+ G
Sbjct: 44  DIFPPHLGKKVISEVVATFLLVFVTCGAASIYGEDNRRISQLGQSVAGGLIVTVMIYATG 103

Query: 92  HISGAHMNPAVTIAFAAVRRFPWRQVIFFFLLCFSPQFLCQFTI 136
           HISGAHMNPAVT++FA  R FPW QV F++   F+      F +
Sbjct: 104 HISGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVL 147

[Close]

BLAST of Cucsat.G13250.T5 vs. ExPASy Swiss-Prot
Match: Q67WJ8 (Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.7e-24
Identity = 54/106 (50.94%), Postives = 77/106 (72.64%), Query Frame = 0


Query: 30  FDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTKLGASITCGLIVTVMIYS 89
           F +++PP   +K+++EV+AT+LLVFV+CG A++ G D   +++LG S+  GLIVTVMIY+
Sbjct: 42  FADIFPPNLLKKVISEVVATFLLVFVTCGAASIYGEDMKRISQLGQSVVGGLIVTVMIYA 101

Query: 90  VGHISGAHMNPAVTIAFAAVRRFPWRQVIFFFLLCFSPQFLCQFTI 136
            GHISGAHMNPAVT++FA  R FPW QV F++   F+      F +
Sbjct: 102 TGHISGAHMNPAVTLSFAFFRHFPWIQVPFYWAAQFTGAMCAAFVL 147

[Close]

BLAST of Cucsat.G13250.T5 vs. NCBI nr
Match: APT69294.1 (LSi6 [Cucumis sativus var. sativus] >KGN59571.1 hypothetical protein Csa_000858 [Cucumis sativus])

HSP 1 Score: 236 bits (602), Expect = 1.19e-75
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117
           ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV
Sbjct: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117

[Close]

BLAST of Cucsat.G13250.T5 vs. NCBI nr
Match: NP_001292702.1 (aquaporin NIP2-1-like [Cucumis sativus] >ACU29604.1 Si transport-like protein 2 [Cucumis sativus])

HSP 1 Score: 234 bits (598), Expect = 4.83e-75
Identity = 116/117 (99.15%), Postives = 116/117 (99.15%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117
           ALSGSDEPVVTKLGASITCGLIVTVMIYS GHISGAHMNPAVTIAFAAVRRFPWRQV
Sbjct: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSAGHISGAHMNPAVTIAFAAVRRFPWRQV 117

[Close]

BLAST of Cucsat.G13250.T5 vs. NCBI nr
Match: NP_001380719.1 (aquaporin NIP2-2 [Cucumis melo])

HSP 1 Score: 217 bits (552), Expect = 4.61e-68
Identity = 107/117 (91.45%), Postives = 113/117 (96.58%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARR+D+EEAFIALGNECSDPQP LFRDRFDE+YPP FSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRNDNEEAFIALGNECSDPQPSLFRDRFDEVYPPGFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117
           ALSGSDE  V+KLGASITCGLIVTVMIY+VGHISGAHMNPAVTIAFAAVRRFPW+QV
Sbjct: 61  ALSGSDEQEVSKLGASITCGLIVTVMIYAVGHISGAHMNPAVTIAFAAVRRFPWKQV 117

[Close]

BLAST of Cucsat.G13250.T5 vs. NCBI nr
Match: XP_038906096.1 (aquaporin NIP2-2-like [Benincasa hispida])

HSP 1 Score: 177 bits (450), Expect = 1.52e-52
Identity = 93/113 (82.30%), Postives = 98/113 (86.73%), Query Frame = 0


Query: 5   SDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSG 64
           S +EEA I  G+ECSDPQ   FRDRF ELYPPEFSRKLVAEVIATYLLVFV+CG AALS 
Sbjct: 13  SSNEEALI--GSECSDPQQSFFRDRFYELYPPEFSRKLVAEVIATYLLVFVTCGAAALSV 72

Query: 65  SDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117
           SDE  V+KLGASIT GLIVTVMIY+VGHISGAHMNPAVT AFAAVRRFPW QV
Sbjct: 73  SDEREVSKLGASITGGLIVTVMIYAVGHISGAHMNPAVTFAFAAVRRFPWNQV 123

[Close]

BLAST of Cucsat.G13250.T5 vs. NCBI nr
Match: XP_023528272.1 (aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 172 bits (437), Expect = 1.28e-50
Identity = 87/110 (79.09%), Postives = 96/110 (87.27%), Query Frame = 0


Query: 8   EEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDE 67
           EEAFI++G + SDP    FRDRF +L PPEFSRKLVAEVIATYLLVFV+CG AALS SDE
Sbjct: 11  EEAFISVGTDYSDPHQSFFRDRFQQLCPPEFSRKLVAEVIATYLLVFVTCGAAALSVSDE 70

Query: 68  PVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117
             V+KLGAS+T GLIVTVMIY+VGHISGAHMNPAVT AFAAVRRFPW+QV
Sbjct: 71  RQVSKLGASMTGGLIVTVMIYAVGHISGAHMNPAVTFAFAAVRRFPWKQV 120

[Close]

BLAST of Cucsat.G13250.T5 vs. ExPASy TrEMBL
Match: A0A1L7B7J9 (LSi6 OS=Cucumis sativus var. sativus OX=869827 GN=LSi6 PE=2 SV=1)

HSP 1 Score: 236 bits (602), Expect = 5.77e-76
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117
           ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV
Sbjct: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117

[Close]

BLAST of Cucsat.G13250.T5 vs. ExPASy TrEMBL
Match: A0A0A0LHR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G826650 PE=3 SV=1)

HSP 1 Score: 236 bits (602), Expect = 5.77e-76
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117
           ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV
Sbjct: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117

[Close]

BLAST of Cucsat.G13250.T5 vs. ExPASy TrEMBL
Match: G8XUV4 (Si transport-like protein 2 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 234 bits (598), Expect = 2.34e-75
Identity = 116/117 (99.15%), Postives = 116/117 (99.15%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117
           ALSGSDEPVVTKLGASITCGLIVTVMIYS GHISGAHMNPAVTIAFAAVRRFPWRQV
Sbjct: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSAGHISGAHMNPAVTIAFAAVRRFPWRQV 117

[Close]

BLAST of Cucsat.G13250.T5 vs. ExPASy TrEMBL
Match: A0A1S3B8P6 (aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487003 PE=3 SV=1)

HSP 1 Score: 217 bits (552), Expect = 2.23e-68
Identity = 107/117 (91.45%), Postives = 113/117 (96.58%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARR+D+EEAFIALGNECSDPQP LFRDRFDE+YPP FSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRNDNEEAFIALGNECSDPQPSLFRDRFDEVYPPGFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117
           ALSGSDE  V+KLGASITCGLIVTVMIY+VGHISGAHMNPAVTIAFAAVRRFPW+QV
Sbjct: 61  ALSGSDEQEVSKLGASITCGLIVTVMIYAVGHISGAHMNPAVTIAFAAVRRFPWKQV 117

[Close]

BLAST of Cucsat.G13250.T5 vs. ExPASy TrEMBL
Match: A0A6J1J0H5 (aquaporin NIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111482345 PE=3 SV=1)

HSP 1 Score: 171 bits (434), Expect = 1.76e-50
Identity = 86/110 (78.18%), Postives = 96/110 (87.27%), Query Frame = 0


Query: 8   EEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDE 67
           EEAFI++G + SDP    FRDRF +L PPEFSRKLVAEVIATYLLVFV+CG AALS SDE
Sbjct: 11  EEAFISVGTDYSDPHQSFFRDRFQQLCPPEFSRKLVAEVIATYLLVFVTCGAAALSVSDE 70

Query: 68  PVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQV 117
             V+KLGAS+T GLIVTVMIY+VGHISGAHMNPAVT AFAAV+RFPW+QV
Sbjct: 71  RQVSKLGASMTGGLIVTVMIYAVGHISGAHMNPAVTFAFAAVKRFPWKQV 120

The following BLAST results are available for this feature:

BLAST of Cucsat.G13250.T5 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q19KC1	4.2e-27	49.59	Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2	[more]
Q6Z2T3	3.0e-25	55.77	Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1	[more]
Q9AT74	3.4e-24	50.96	Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1	[more]
Q9ATN2	4.4e-24	50.96	Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1	[more]
Q67WJ8	5.7e-24	50.94	Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1	[more]

BLAST of Cucsat.G13250.T5 vs. NCBI nr


Match Name	E-value	Identity	Description
APT69294.1	1.19e-75	100.00	LSi6 [Cucumis sativus var. sativus] >KGN59571.1 hypothetical protein Csa_000858 ...	[more]
NP_001292702.1	4.83e-75	99.15	aquaporin NIP2-1-like [Cucumis sativus] >ACU29604.1 Si transport-like protein 2 ...	[more]
NP_001380719.1	4.61e-68	91.45	aquaporin NIP2-2 [Cucumis melo]	[more]
XP_038906096.1	1.52e-52	82.30	aquaporin NIP2-2-like [Benincasa hispida]	[more]
XP_023528272.1	1.28e-50	79.09	aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G13250.T5 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1L7B7J9	5.77e-76	100.00	LSi6 OS=Cucumis sativus var. sativus OX=869827 GN=LSi6 PE=2 SV=1	[more]
A0A0A0LHR6	5.77e-76	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G826650 PE=3 SV=1	[more]
G8XUV4	2.34e-75	99.15	Si transport-like protein 2 OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A1S3B8P6	2.23e-68	91.45	aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487003 PE=3 SV=1	[more]
A0A6J1J0H5	1.76e-50	78.18	aquaporin NIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111482345 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 79..103 score: 49.97 coord: 40..59 score: 37.31
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 36..122 e-value: 7.4E-21 score: 74.9
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 22..138 e-value: 5.4E-27 score: 96.2
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 32..122
None	No IPR available	PANTHER	PTHR45724:SF30	MAJOR INTRINSIC PROTEIN, AQUAPORIN-LIKE PROTEIN-RELATED	coord: 29..121
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 29..121
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 97..105

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G13250	Cucsat.G13250	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13250.T5.E1	Cucsat.G13250.T5.E1	exon
Cucsat.G13250.T5.E2	Cucsat.G13250.T5.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13250.T5.C1	Cucsat.G13250.T5.C1	CDS
Cucsat.G13250.T5.C2	Cucsat.G13250.T5.C2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13250.T5	Cucsat.G13250.T5-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0015267	channel activity