Cucsat.G13203.T3 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G13203.T3
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	WAT1-related protein
Location	ctg1838: 2166720 .. 2169539 (+)
RNA-Seq Expression	Cucsat.G13203.T3
Synteny	Cucsat.G13203.T3

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTCAAACTATTTAAAGTTGTCTATAAAATACATATTTGGTGTTTCAAGGCACTTTATCGTAAGTATTTTTCGAAAGTGTTAACTCCTCTCTTTTCATTGTTCTTCTTGTTCTTTGATTTTAGCCATTATAGCTATAGTTTTCTTCTTCTACATATTTATATTCAAAAACAACATCCCTTCTTTTTTTTTTAACATAACCACACACGAAGTCCATGGGATTATTCTTTGGCCATTTCTTCCAAAATGCAATGCCATACATCGCAGTCATCTTCCTCCAATTCGGCTACGCCGGACTGAACATCCTCTCTGCCATCGCCCTCAGTGGTGGTATCAGTCACTACGTTCTTGTCGTCTACCGTCAGGTCTTTGCTACTGCGATCATGGCGCCCTTTGCTCTCATTCTCGAGAGGTGCGTATAATCATATATTCCTCTGCTATCATATATTTCTACAACATACTACAATAACTATTGTTTTGCTCAATTTCACTGAAAATATACTATTTTTTACCTTGTGGTTAACGCTTTTAAAATAATTATGGATATAGTTGTGGTTAACTATATTGCATGTTAGATTAAATTAAGAAGTATTTTGGAGGTAGATTAATAAATAATTTGTTTTGCCAAACAACGACTTAGGTGGGATTTTTCTATAATCACTTTTTTCTGTTTGCAAACGCATTCCAAGCGTTATTTCCCGACAACTCATTCATCCATTCTTTTTTTTTTTCAGGAAATTTAGACCAAAGATTACAGTTAAGATTTTCATTCAGATGTTCGTTCTTGCTCTTCTTGGGTTAGTATCAAAACTTTGGCTTTTAATTTCTTAATAATTATTATTGTTATTGAGGTCACCTTCCAATCATTTCTTTCTCTTTTTTTTTGGGTTTTTATAAGAAAGTCTATTTTCTCTCTTTTTTTTTCTAATTTTCAAAAGTTGGGTGTGTGGTTTCTGAAATTATTGGTAAAAAACAAAGCATAAAATTGAGAGGTGGAACAAGTTCTTCTACATGTAATTTTCAAAAGTTAAAAATAAAAAATCAAATGGTCATGAAGCAACAACTTATCGTTTTCTTCAAGATTACCTTTTTACTTTACCTCTATATATATCTTCCATATGCCATTGATTTCTGTCTCAACAGGCCATTGCTTGATCAAAACTTATACTACATGGGGCTAAAATTGACATCTCCAACAATCGCTTGCGCCATAGGCAACATGCTTCCTTCAATGACATTTGCTATGGCAGTCATTTGCAAGTACTTTAAACCTTGGTTACCCCCATCCTCTCGTTTCGAATAAAATAATAAAAAGTGTAAAAAATATGAATAAACATACTAATTTTTATTGGTCAAGTTTATTTTAACCTTGGGCATTCAAACCTTCTATAATCTTATTTCTTTGTCGATCTATCAGGATAGAAAAGTTAGATATGAAAAGAGTGGGATGTCGAGCGAAGTTGTTGGGAACAATAGTGACCCTATGTGGAGCCATGTTGATGACATTTTATAAAGGAAGTATCGTCAACTTTCTCGGTACAAAGCACGGCCGCCAACCGAACATACCATCCACGGCCGTGCACAACCACCACAACAAGGGAGAGTACATCAAAGGCTCCATTTTGCTCATCATCTCCGTTTTTGCTTGGGCAGCCTTCTTCGTCCTTCAAGCAATAACCTTGAGAAAATACACAGCTCATCTTTCTCTCACCGCCTTGGTTTGCTTTCTGGGAACCCTTCAGGCTATTGTTGCCACCCTTGCCATGGAGCGTCGCCTTTCCGTGTGGACCATCGGTTGGGATTGGAACCTTCTAGCATCTGCTTATGCGGTAAGAAAGACAATTATTATCAGATATTTTTTTATCTTTAAAATAAATGCACGATGCCTCACAATATTGAGATACAAATTATGTAGGGAATTGTGACGACAGGGGTCGCATATTATGTTCAAGGTATGGTTATGAAAAGAAGAGGGCCAGTTTTTGTTACAGCTTTTGGTCCTATGGTAGTAGTGATTGTTGCCTTCATGGGTCATTTCATCTTGGCCGAAGAGATTTATGTTGGAGGGTAACTCATCAATTTCTTTCTGTCTTTCTTGTCTATATAATTACTTTTCTCACTAAGATAATAATCCTAATTATATGATATTTTTATGTTTGGAATTTTCAGGATAATTGGAACAGTGGTGATTGTGATAGGGTTATACTTTGTTTTGTGGGGAATGTATAAGGAAAGCAAAGAGAAGAAGGAAGAAGTTAATGGAGAGATTATTGTTGAAGCAATAATAGACGAAGGTGATCATCATGATCATCAATTGCCTATGATAAATGAAGGTATTGAATTAGCAATTGATCAAAAGAAAGAGGGACATTTGGCCATTACTACTATTCCATCCCTTCATGGCTTCACCTAATTACATGGGAAAAGAGGCCTCAAGACACTAATCAAACCAAATCAAAAGTCAATTAAAAAAATATTGAAGAACACTAACAAAGAAATTATGGCTAATTGAGAAATTAAAAAGGGAGGAGAAGAATATTGATGATTAAACACTCTTTTCTTTAAATTTGCCTTTTTTATATTCTATTTCATCCTCCAATTTTCTCTTTTAACTTTAGTTTTCTCTCTCTAATCTCTTTTTGGTTGGGTATAATAATGGGTCTATTATAATTGGCCCTTTAAAAAATTCTCTACAGCTTCTGTTTTTTAGGTTTTTAATGCAAGTTTGTATTGACTCTTTTTAAAGTATTGTTCATTGATTCTAAAGGTTTTAAGGTGTAGATTAATGTACAACTATTAGTTAATAGTTTAAATTGATACTC

Coding sequence (CDS)

ATGAAAAGAGTGGGATGTCGAGCGAAGTTGTTGGGAACAATAGTGACCCTATGTGGAGCCATGTTGATGACATTTTATAAAGGAAGTATCGTCAACTTTCTCGGTACAAAGCACGGCCGCCAACCGAACATACCATCCACGGCCGTGCACAACCACCACAACAAGGGAGAGTACATCAAAGGCTCCATTTTGCTCATCATCTCCGTTTTTGCTTGGGCAGCCTTCTTCGTCCTTCAAGCAATAACCTTGAGAAAATACACAGCTCATCTTTCTCTCACCGCCTTGGTTTGCTTTCTGGGAACCCTTCAGGCTATTGTTGCCACCCTTGCCATGGAGCGTCGCCTTTCCGTGTGGACCATCGGTTGGGATTGGAACCTTCTAGCATCTGCTTATGCGGGAATTGTGACGACAGGGGTCGCATATTATGTTCAAGGTATGGTTATGAAAAGAAGAGGGCCAGTTTTTGTTACAGCTTTTGGTCCTATGGTAGTAGTGATTGTTGCCTTCATGGGTCATTTCATCTTGGCCGAAGAGATTTATGTTGGAGGGATAATTGGAACAGTGGTGATTGTGATAGGGTTATACTTTGTTTTGTGGGGAATGTATAAGGAAAGCAAAGAGAAGAAGGAAGAAGTTAATGGAGAGATTATTGTTGAAGCAATAATAGACGAAGGTGATCATCATGATCATCAATTGCCTATGATAAATGAAGGTATTGAATTAGCAATTGATCAAAAGAAAGAGGGACATTTGGCCATTACTACTATTCCATCCCTTCATGGCTTCACCTAA

Protein sequence

MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIKGSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTIGWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIYVGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIELAIDQKKEGHLAITTIPSLHGFT

Homology

[Close]

BLAST of Cucsat.G13203.T3 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.1e-58
Identity = 117/210 (55.71%), Postives = 155/210 (73.81%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGR---QPNIPSTAVHNHHNKGE 60
           +K++ C+AK+ GT+VT+ GAMLMT YKG IV    TK+       +  +T+  N  +  E
Sbjct: 136 LKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKE 195

Query: 61  YIKGSILLIISVFAWAAFFVLQAITLRKYTAH-LSLTALVCFLGTLQAIVATLAMERRLS 120
           ++KGSILLI +  AWA+ FVLQA  L+ Y  H LSLT L+CF+GTLQA+  T  ME   S
Sbjct: 196 FLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPS 255

Query: 121 VWTIGWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILA 180
            W IGWD NLLA+AY+GIV + ++YYVQG+VMK+RGPVF TAF P+++VIVA MG F+LA
Sbjct: 256 AWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLA 315

Query: 181 EEIYVGGIIGTVVIVIGLYFVLWGMYKESK 207
           E+I++GG+IG V+IVIGLY VLWG  KE++
Sbjct: 316 EKIFLGGVIGAVLIVIGLYAVLWGKQKENQ 345

[Close]

BLAST of Cucsat.G13203.T3 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 7.7e-53
Identity = 112/261 (42.91%), Postives = 174/261 (66.67%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 60
           M++V C  K++GT+VT+ G++LM FYKG  +NF    H    + P TA        +Y+K
Sbjct: 129 MRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFF-RSHLTAASSPPTA--------DYLK 188

Query: 61  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 120
            ++ L+++  +WA+FFVLQA TL+KY+AHLS++ +VCF+GTLQ++     ME   S   I
Sbjct: 189 AAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNI 248

Query: 121 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 180
           G+D NLLASAYAGI+++ +AYYVQG++M+R+GPVFVTAF P++VVIV+ M  F+L + IY
Sbjct: 249 GFDMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIY 308

Query: 181 VGGIIGTVVIVIGLYFVLWGMY--KESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEG 240
           +GG+IG VV+++G+Y VLWG +   + +E + E N   +V      G++    +P I+E 
Sbjct: 309 LGGVIGVVVLMVGVYAVLWGKHVDDDGEETRHEDN---VVAVKCCSGNNGLTIMPKIDEA 368

Query: 241 IELAIDQKKEGHLAITTIPSL 260
            E  ++  K      +++P +
Sbjct: 369 DEEDVETGKATSEKESSVPEV 377

[Close]

BLAST of Cucsat.G13203.T3 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 3.5e-45
Identity = 98/218 (44.95%), Postives = 143/218 (65.60%), Query Frame = 0


Query: 2   KRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFL-------------GTKHGRQPNIPSTA 61
           K+V   AK++GT++T+ GA+LMT YKG IV+F+             G  HG      + A
Sbjct: 130 KKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHG---GAGAAA 189

Query: 62  VHNHHNKGEYIKGSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVAT 121
           +  H     +I G+++L+   F WA FF+LQ+ TL++Y A LSLT L+C +GTL+    +
Sbjct: 190 MDKH-----WIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVS 249

Query: 122 LAMERRLSVWTIGWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVA 181
           L   R LS W IG+D NL A+AY+G++ +GVAYYVQG+VM+ RGPVFV  F P+ VVI A
Sbjct: 250 LVTVRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITA 309

Query: 182 FMGHFILAEEIYVGGIIGTVVIVIGLYFVLWGMYKESK 207
            +G  +L+E I++G +IGT+ I++GLY V+WG  K+ +
Sbjct: 310 ALGVVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKDKR 339

[Close]

BLAST of Cucsat.G13203.T3 vs. ExPASy Swiss-Prot
Match: Q9LXX8 (WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 5.9e-45
Identity = 102/225 (45.33%), Postives = 151/225 (67.11%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLG---TKHGRQPNIPSTAVHNHHNKGE 60
           M++V  +AK++GT+V + GAMLM  +K  ++ FL    T H   P             GE
Sbjct: 129 MRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFLRSHLTGHALSP------------AGE 188

Query: 61  -YIKGSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLS 120
            Y+K ++ L+I+ F+WA+FFVLQA TL++Y++HLSL+ +VCF+GTLQ+   T  ME  LS
Sbjct: 189 DYLKATVFLLIASFSWASFFVLQAATLKRYSSHLSLSTMVCFMGTLQSTALTFVMEPNLS 248

Query: 121 VWTIGWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILA 180
            W IG+D NLLASAYAGI+++ +AYYVQGM+ K++  +FVTAF P+VV+I + +G  IL 
Sbjct: 249 AWNIGFDMNLLASAYAGIMSSSIAYYVQGMMTKQKSVIFVTAFNPLVVIIGSIIGFLILN 308

Query: 181 EEIYVGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAI 222
           + + +GG++G  ++V+G+  VLWG  KE    +EE   E  VE +
Sbjct: 309 QTLNLGGVLGMAILVVGVCTVLWG--KEGDIDEEENIEEKFVEIV 339

[Close]

BLAST of Cucsat.G13203.T3 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 3.2e-43
Identity = 97/218 (44.50%), Postives = 140/218 (64.22%), Query Frame = 0


Query: 4   VGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVH--NHHNKGEYIKG 63
           V  +AK++GT+V L GA++MT YKG ++    +     PN+     H  N  +   ++ G
Sbjct: 134 VRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWS----NPNMDQQNGHTNNSQDHNNWVVG 193

Query: 64  SILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTIG 123
           ++L+++   AW+ F+VLQ+IT++ Y A LSL+AL+C  G +Q+    L +ER  S W +G
Sbjct: 194 TLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVERHPSGWAVG 253

Query: 124 WDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIYV 183
           WD  L A  Y GIV++G+ YYVQGMVMK RGPVFVTAF P+ +++VA +  FIL E+I+ 
Sbjct: 254 WDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIASFILHEQIHF 313

Query: 184 GGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVE 220
           G +IG  VI  GLY V+WG     K K  EV+G  I+E
Sbjct: 314 GCVIGGAVIAAGLYMVVWG-----KGKDYEVSGLDILE 342

[Close]

BLAST of Cucsat.G13203.T3 vs. NCBI nr
Match: XP_031737592.1 (LOW QUALITY PROTEIN: WAT1-related protein At5g07050 [Cucumis sativus])

HSP 1 Score: 490 bits (1261), Expect = 1.07e-172
Identity = 253/259 (97.68%), Postives = 255/259 (98.46%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 60
           +KRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHG QPNIPSTAVHNHHNKGEYIK
Sbjct: 81  LKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGHQPNIPSTAVHNHHNKGEYIK 140

Query: 61  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 120
           GSILLIISVF WAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI
Sbjct: 141 GSILLIISVFVWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 200

Query: 121 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 180
           GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY
Sbjct: 201 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 260

Query: 181 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE 240
           VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE
Sbjct: 261 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE 320

Query: 241 LAIDQKKEGHLAITTIPSL 259
           LAIDQKKEG L ITTIPS+
Sbjct: 321 LAIDQKKEG-LTITTIPSM 338

[Close]

BLAST of Cucsat.G13203.T3 vs. NCBI nr
Match: XP_031737517.1 (WAT1-related protein At5g07050 isoform X2 [Cucumis sativus] >KAE8651169.1 hypothetical protein Csa_000888 [Cucumis sativus])

HSP 1 Score: 476 bits (1225), Expect = 1.05e-166
Identity = 250/263 (95.06%), Postives = 250/263 (95.06%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 60
           MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK
Sbjct: 132 MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 191

Query: 61  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 120
           GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI
Sbjct: 192 GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 251

Query: 121 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 180
           GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY
Sbjct: 252 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 311

Query: 181 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE 240
           VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIID             EGIE
Sbjct: 312 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIID-------------EGIE 371

Query: 241 LAIDQKKEGHLAITTIPSLHGFT 263
           LAIDQKKEGHLAITTIPSLHGFT
Sbjct: 372 LAIDQKKEGHLAITTIPSLHGFT 381

[Close]

BLAST of Cucsat.G13203.T3 vs. NCBI nr
Match: XP_031737516.1 (WAT1-related protein At5g07050 isoform X1 [Cucumis sativus])

HSP 1 Score: 476 bits (1225), Expect = 1.25e-166
Identity = 250/263 (95.06%), Postives = 250/263 (95.06%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 60
           MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK
Sbjct: 137 MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 196

Query: 61  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 120
           GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI
Sbjct: 197 GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 256

Query: 121 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 180
           GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY
Sbjct: 257 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 316

Query: 181 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE 240
           VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIID             EGIE
Sbjct: 317 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIID-------------EGIE 376

Query: 241 LAIDQKKEGHLAITTIPSLHGFT 263
           LAIDQKKEGHLAITTIPSLHGFT
Sbjct: 377 LAIDQKKEGHLAITTIPSLHGFT 386

[Close]

BLAST of Cucsat.G13203.T3 vs. NCBI nr
Match: KAE8651167.1 (hypothetical protein Csa_001256 [Cucumis sativus])

HSP 1 Score: 451 bits (1159), Expect = 7.79e-158
Identity = 238/255 (93.33%), Postives = 240/255 (94.12%), Query Frame = 0


Query: 5   GCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIKGSIL 64
           G   +LLGTIVTLCGAMLMTFYKGSIVNFLGTKHG QPNIPSTAVHNHHNKGEYIKGSIL
Sbjct: 50  GNHLQLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGHQPNIPSTAVHNHHNKGEYIKGSIL 109

Query: 65  LIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTIGWDW 124
           LIISVF WAAFFVLQAITLRKYTAHLSLTAL        AIVATLAMERRLSVWTIGWDW
Sbjct: 110 LIISVFVWAAFFVLQAITLRKYTAHLSLTAL--------AIVATLAMERRLSVWTIGWDW 169

Query: 125 NLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIYVGGI 184
           NLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIYVGGI
Sbjct: 170 NLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIYVGGI 229

Query: 185 IGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIELAID 244
           IGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIELAID
Sbjct: 230 IGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIELAID 289

Query: 245 QKKEGHLAITTIPSL 259
           QKKEG L ITTIPS+
Sbjct: 290 QKKEG-LTITTIPSM 295

[Close]

BLAST of Cucsat.G13203.T3 vs. NCBI nr
Match: XP_016899669.1 (PREDICTED: WAT1-related protein At5g07050-like [Cucumis melo] >TYK25504.1 WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 432 bits (1110), Expect = 7.96e-149
Identity = 223/259 (86.10%), Postives = 240/259 (92.66%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 60
           +KRV CRAKLLGTIVTL GAMLMTFYKG+ VNFLGTKHGRQPN PSTAV NHHN+GEYIK
Sbjct: 131 LKRVRCRAKLLGTIVTLGGAMLMTFYKGNAVNFLGTKHGRQPNTPSTAVLNHHNQGEYIK 190

Query: 61  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 120
           GSILLIIS  AWAAFF+LQ ITLRKYTAHLSLT LVCFLGTLQA VAT A+ERRLS W I
Sbjct: 191 GSILLIISTLAWAAFFILQTITLRKYTAHLSLTTLVCFLGTLQATVATFAIERRLSAWAI 250

Query: 121 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 180
           GWDWNLLASAYAGIV++GVAYYVQG+VMK RGPVFVTAFGPMVVVIVAFMGHFILAEEIY
Sbjct: 251 GWDWNLLASAYAGIVSSGVAYYVQGLVMKTRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 310

Query: 181 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE 240
           VGG+IG+VVI+IGLYFVLWG YKES+EKKEEV GEIIVEAI++EGDHHDHQLP++NEGIE
Sbjct: 311 VGGVIGSVVIIIGLYFVLWGKYKESREKKEEVIGEIIVEAIMEEGDHHDHQLPIVNEGIE 370

Query: 241 LAIDQKKEGHLAITTIPSL 259
           LA DQKKEG LAIT IPS+
Sbjct: 371 LATDQKKEG-LAIT-IPSM 387

[Close]

BLAST of Cucsat.G13203.T3 vs. ExPASy TrEMBL
Match: A0A0A0LES5 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_3G818280 PE=3 SV=1)

HSP 1 Score: 491 bits (1265), Expect = 1.21e-174
Identity = 254/259 (98.07%), Postives = 256/259 (98.84%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 60
           +KRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHG QPNIPSTAVHNHHNKGEYIK
Sbjct: 16  LKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGHQPNIPSTAVHNHHNKGEYIK 75

Query: 61  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 120
           GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI
Sbjct: 76  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 135

Query: 121 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 180
           GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY
Sbjct: 136 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 195

Query: 181 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE 240
           VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE
Sbjct: 196 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE 255

Query: 241 LAIDQKKEGHLAITTIPSL 259
           LAIDQKKEG L ITTIPS+
Sbjct: 256 LAIDQKKEG-LTITTIPSM 273

[Close]

BLAST of Cucsat.G13203.T3 vs. ExPASy TrEMBL
Match: A0A5D3DPE8 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006530 PE=3 SV=1)

HSP 1 Score: 432 bits (1110), Expect = 3.85e-149
Identity = 223/259 (86.10%), Postives = 240/259 (92.66%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 60
           +KRV CRAKLLGTIVTL GAMLMTFYKG+ VNFLGTKHGRQPN PSTAV NHHN+GEYIK
Sbjct: 131 LKRVRCRAKLLGTIVTLGGAMLMTFYKGNAVNFLGTKHGRQPNTPSTAVLNHHNQGEYIK 190

Query: 61  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 120
           GSILLIIS  AWAAFF+LQ ITLRKYTAHLSLT LVCFLGTLQA VAT A+ERRLS W I
Sbjct: 191 GSILLIISTLAWAAFFILQTITLRKYTAHLSLTTLVCFLGTLQATVATFAIERRLSAWAI 250

Query: 121 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 180
           GWDWNLLASAYAGIV++GVAYYVQG+VMK RGPVFVTAFGPMVVVIVAFMGHFILAEEIY
Sbjct: 251 GWDWNLLASAYAGIVSSGVAYYVQGLVMKTRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 310

Query: 181 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE 240
           VGG+IG+VVI+IGLYFVLWG YKES+EKKEEV GEIIVEAI++EGDHHDHQLP++NEGIE
Sbjct: 311 VGGVIGSVVIIIGLYFVLWGKYKESREKKEEVIGEIIVEAIMEEGDHHDHQLPIVNEGIE 370

Query: 241 LAIDQKKEGHLAITTIPSL 259
           LA DQKKEG LAIT IPS+
Sbjct: 371 LATDQKKEG-LAIT-IPSM 387

[Close]

BLAST of Cucsat.G13203.T3 vs. ExPASy TrEMBL
Match: A0A1S4DUL2 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103486872 PE=3 SV=1)

HSP 1 Score: 432 bits (1110), Expect = 3.85e-149
Identity = 223/259 (86.10%), Postives = 240/259 (92.66%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 60
           +KRV CRAKLLGTIVTL GAMLMTFYKG+ VNFLGTKHGRQPN PSTAV NHHN+GEYIK
Sbjct: 131 LKRVRCRAKLLGTIVTLGGAMLMTFYKGNAVNFLGTKHGRQPNTPSTAVLNHHNQGEYIK 190

Query: 61  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 120
           GSILLIIS  AWAAFF+LQ ITLRKYTAHLSLT LVCFLGTLQA VAT A+ERRLS W I
Sbjct: 191 GSILLIISTLAWAAFFILQTITLRKYTAHLSLTTLVCFLGTLQATVATFAIERRLSAWAI 250

Query: 121 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 180
           GWDWNLLASAYAGIV++GVAYYVQG+VMK RGPVFVTAFGPMVVVIVAFMGHFILAEEIY
Sbjct: 251 GWDWNLLASAYAGIVSSGVAYYVQGLVMKTRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 310

Query: 181 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE 240
           VGG+IG+VVI+IGLYFVLWG YKES+EKKEEV GEIIVEAI++EGDHHDHQLP++NEGIE
Sbjct: 311 VGGVIGSVVIIIGLYFVLWGKYKESREKKEEVIGEIIVEAIMEEGDHHDHQLPIVNEGIE 370

Query: 241 LAIDQKKEGHLAITTIPSL 259
           LA DQKKEG LAIT IPS+
Sbjct: 371 LATDQKKEG-LAIT-IPSM 387

[Close]

BLAST of Cucsat.G13203.T3 vs. ExPASy TrEMBL
Match: A0A0A0LBM2 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_3G819280 PE=3 SV=1)

HSP 1 Score: 430 bits (1105), Expect = 1.08e-148
Identity = 232/259 (89.58%), Postives = 236/259 (91.12%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 60
           +KRV CRAKLLGTIVTL GAMLMTFYKGS+VNFLGTKHG QPNIPSTAV  HHNKGEYIK
Sbjct: 132 LKRVRCRAKLLGTIVTLGGAMLMTFYKGSVVNFLGTKHGPQPNIPSTAVLKHHNKGEYIK 191

Query: 61  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 120
           GSILLIIS  AWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI
Sbjct: 192 GSILLIISNLAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 251

Query: 121 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 180
           GWDWNLLASAYAGIVTTGVAYYVQG+VMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY
Sbjct: 252 GWDWNLLASAYAGIVTTGVAYYVQGLVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 311

Query: 181 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEGIE 240
           VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAII              EGIE
Sbjct: 312 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAII-------------GEGIE 371

Query: 241 LAIDQKKEGHLAITTIPSL 259
           LAIDQKKEG L ITTIPS+
Sbjct: 372 LAIDQKKEG-LTITTIPSM 376

[Close]

BLAST of Cucsat.G13203.T3 vs. ExPASy TrEMBL
Match: E5GBP6 (WAT1-related protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 409 bits (1051), Expect = 1.15e-140
Identity = 206/238 (86.55%), Postives = 222/238 (93.28%), Query Frame = 0


Query: 1   MKRVGCRAKLLGTIVTLCGAMLMTFYKGSIVNFLGTKHGRQPNIPSTAVHNHHNKGEYIK 60
           +KRV CRAKLLGTIVTL GAMLMTFYKG+ VNFLGTKHGRQPN PSTAV NHHN+GEYIK
Sbjct: 131 LKRVRCRAKLLGTIVTLGGAMLMTFYKGNAVNFLGTKHGRQPNTPSTAVLNHHNQGEYIK 190

Query: 61  GSILLIISVFAWAAFFVLQAITLRKYTAHLSLTALVCFLGTLQAIVATLAMERRLSVWTI 120
           GSILLIIS  AWAAFF+LQ ITLRKYTAHLSLT LVCFLGTLQA VAT A+ERRLS W I
Sbjct: 191 GSILLIISTLAWAAFFILQTITLRKYTAHLSLTTLVCFLGTLQATVATFAIERRLSAWAI 250

Query: 121 GWDWNLLASAYAGIVTTGVAYYVQGMVMKRRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 180
           GWDWNLLASAYAGIV++GVAYYVQG+VMK RGPVFVTAFGPMVVVIVAFMGHFILAEEIY
Sbjct: 251 GWDWNLLASAYAGIVSSGVAYYVQGLVMKTRGPVFVTAFGPMVVVIVAFMGHFILAEEIY 310

Query: 181 VGGIIGTVVIVIGLYFVLWGMYKESKEKKEEVNGEIIVEAIIDEGDHHDHQLPMINEG 238
           VGG+IG+VVI+IGLYFVLWG YKES+EKKEEV GEIIVEAI++EGDHHDHQLP++NEG
Sbjct: 311 VGGVIGSVVIIIGLYFVLWGKYKESREKKEEVIGEIIVEAIMEEGDHHDHQLPIVNEG 368

The following BLAST results are available for this feature:

BLAST of Cucsat.G13203.T3 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FL41	6.1e-58	55.71	WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2...	[more]
F4IJ08	7.7e-53	42.91	WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2...	[more]
F4HZQ7	3.5e-45	44.95	WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2...	[more]
Q9LXX8	5.9e-45	45.33	WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2...	[more]
Q9SUF1	3.2e-43	44.50	WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2...	[more]

BLAST of Cucsat.G13203.T3 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031737592.1	1.07e-172	97.68	LOW QUALITY PROTEIN: WAT1-related protein At5g07050 [Cucumis sativus]	[more]
XP_031737517.1	1.05e-166	95.06	WAT1-related protein At5g07050 isoform X2 [Cucumis sativus] >KAE8651169.1 hypoth...	[more]
XP_031737516.1	1.25e-166	95.06	WAT1-related protein At5g07050 isoform X1 [Cucumis sativus]	[more]
KAE8651167.1	7.79e-158	93.33	hypothetical protein Csa_001256 [Cucumis sativus]	[more]
XP_016899669.1	7.96e-149	86.10	PREDICTED: WAT1-related protein At5g07050-like [Cucumis melo] >TYK25504.1 WAT1-r...	[more]

BLAST of Cucsat.G13203.T3 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LES5	1.21e-174	98.07	WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_3G818280 PE=3 SV=1	[more]
A0A5D3DPE8	3.85e-149	86.10	WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352...	[more]
A0A1S4DUL2	3.85e-149	86.10	WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103486872 PE=3 SV=1	[more]
A0A0A0LBM2	1.08e-148	89.58	WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_3G819280 PE=3 SV=1	[more]
E5GBP6	1.15e-140	86.55	WAT1-related protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 60..198 e-value: 2.0E-13 score: 50.7
None	No IPR available	PANTHER	PTHR31218:SF342	WAT1-RELATED PROTEIN	coord: 1..233
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 101..200
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 1..233

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G13203	Cucsat.G13203	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13203.T3.E1	Cucsat.G13203.T3.E1	exon
Cucsat.G13203.T3.E2	Cucsat.G13203.T3.E2	exon
Cucsat.G13203.T3.E3	Cucsat.G13203.T3.E3	exon
Cucsat.G13203.T3.E4	Cucsat.G13203.T3.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13203.T3.C1	Cucsat.G13203.T3.C1	CDS
Cucsat.G13203.T3.C2	Cucsat.G13203.T3.C2	CDS
Cucsat.G13203.T3.C3	Cucsat.G13203.T3.C3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13203.T3	Cucsat.G13203.T3-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0022857	transmembrane transporter activity