Csor.00g155610.m01 (mRNA) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g155610.m01
TypemRNA
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionEf-hand calcium binding protein
LocationCsor_Chr03: 450805 .. 454236 (-)
Sequence length864
RNA-Seq ExpressionCsor.00g155610.m01
SyntenyCsor.00g155610.m01
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAGGTTACCGAAATCAACCTTCCGGTTACGGTTATGGTTACGGCGGTGGCGGTGGCGGTGGTGGTGGTGGTTACGGAGCTCCACCGCCGACGTCTCAGCCGTACAGTTCTCCACCCTACGGCTCGCAACATCCTCCGCCACAGTCACAGCAGCCGTACGCTCCGGTGGCCGCGCAATATGGCGCACCGTCGGCACCGTATGGATCATCTCCGGGCGAAAAACCGCCGAAGGACAAGCCTAATCAGTCTTACGGCGGATCAGCCAGCGGTGGTCATGGTTATCCACCGCCTTCTGCGTATGGAAGCCCATTCGCCTCACTCATGCCCTCCGCCTTTCCGCCTGGGACTGATCCGAGCATCGTTGCGTGTTTTCAAGTAGCCGATCGGGATAGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATGAGAACTGTTCACCTCCTCATGTTTCATTTCACCAACACAAGCACCAGGAAGATCGGTAATCTCTCTGCTCCCTCTATCGCCTTTTTGTTTGCCCTGGCAACAATAAAATTTCATGTGGCTATGAAAACAAGGAAGAGAGAACCTGTAGGAATCTGGAGGGCCCAGATAGAGAAAAAAAACAAACAGTTAGGCCAGTGGGGCAGGGGAGGCCCTTATCGGGGAATCTACTATTAGTTTAAGCTAATTTGAACATAATCTGATAGAGATCAATCTCTGTTTATAAATTGAACGCTTCATTGTCAAAACTTTCGAGGTGAATTTCATATAATTGCATAGAATTGTTATCTTCCTTTTTAGTGATTATGATTGTTTCTTATTACTGTTAGTTGATTTGTTCATGCTAGGAATCACGACTCTCTATAATGATATGATATTGTTCACTTTGAGCATAAACTCTTGACTTTACTATTGGTTTCCTCAAAAGGCTTACTTATATAAACCCATGATCTTCCTCCTAATTAGCCAATGTGAGACTACTCCGGTTAATAATTCTCAACAATCCTCTCATTGAACAAAGTACATCATAGAGCCTCCCCTGAGGCCTATGGAGCCCTCGGATAGCCTCCCCTTAATCGAGGCTCGACTCCTTTCTTTGGAGCTCCTTTTGTTCGACACTTGAGTCATTTTTTACTACACTTTCGAGGCTCACAATTTCTTTGTTTGACATTTGAGGATTCTATTGACATGACTAGGTTGAGGGCATGACTCTGATACCGTGTTAGGAATCACGACACTCCACAATGGTATGATATTGTCCACTTTGAGCATAAGCTCTCATGACCTTGCTTTTGGTTTCCCCAAAAGACCTCGTACCAATGAAGATATTGTTCCTTACTTGTAAAACCCACGATCTTTCTCTTAATTAGCCAATGTGGGACTACTCCCTTCAATAATTCTCAACCATTCATGATGTTCATGAACGCATTCTTTCTGCTACAAGGTAATCTTTCCTCCATGATGCAAAACAATGACGATGTCATTTGAATTAGAGTGATGGTTAAATTGTCTGTTCTTAATCTGCTCGAATCATCTTATTATCATTATCATTAATTTTTTATGCATTTGGCCTTGAGTGACCCTCATCTGGAAATCCTGCTTTTTATCATTCTATTGATGTTGTCTTTTGCATTTCGTTTCTGAAAAACAGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCTAAGCTGGAGGGTAAGCTGAAGATTTGATATCATTTCAGCCAACATTGTCCAACTGACTAACTATTTATTTGATCTTTTTCAGGGTATTTTTGAGAGATTTGATTCAGACCGAAGTGGAAAAATTGATTCCAGAGAGTTGAAAGAAGCACTTTTCAGTTTGGGCTTTTCTGTCTCACCAATGATCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAATAGCAAGGCCATTGAGTATGATAATTTTATAGAGTACGTTTGTCTTCCTTACTTTTTCTTTTTATAACTGGGAGTTGAAGCTTTGTTCCCACGTACCGGGAGGGCACCCACACCCGACCCAAGGCCCGGACCCGGGAGACATCAAGGGTCTTTGGTATTAGTTTGTCTTCCTTACTCTTGATTTTACGTGGTACTCCTGATGAGAGTTTCTGAGTTACCCTTTAGTGTTGAGTTTTCACTTCACATTAATCACTGTTCTTATCAATTCTTTTTATTTTTGCAATTGCAGGTGTTGTCTTACAGTCAAGGTGAGCTTTTTTTATTCTATACTCTTTAGATGTGATGACATGATCTTTGAATCTGTGTATTTAGTCATGCATATTTGCTTAGTTGTCTTGATTTTGTTCCACTCATGTTGATGCCTACGAATACCCCGAATACTTTTGTAACAGCCCAAGCCCACCGCTAGCCGATATTGTCTGTTTCGGCCCGTTACATATTGCCGTCAACCTCATGATTTTAAAATGCGTCCTACTAGGAAGAGGTTTTCACACCCTTATAAAGAATGCTTCGTTCCCCTCTCCAACCGATGTGAGATCTCACAATTCACTCCCTTTTTGGGCTAGCGTTCTCGTTGACACTCGTTCCCTTCTCCGATCGATGTGGGATCTCATAATCCACTCCCCTTCGGGACTCAGTATCCTTGCTAGCACACTGCTCGATGCCTAGCTTTGATACCATTTGTAGCTGCCTAAACCCACCACTAGCAATATCCGTTCGGCCCATGACGTATTGCCATTAGCCCCACGGTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCTACACCCTTATAAACAATGTTTCGTTCCCTTCTCCAACCAATGTGGTATCTCATAATCCACCTCCCTTCGAGGCCAGCGTCCTCGCTGACACTCGTTCCACTCTCCAATCGACGTAGGATCTCACAATCAATTACACCATTTGAACACCCATGGCTATCAAGTAGTTACTTACCATCTAATTTCACTACGAGAAATTACTACTATCGTTTTTTGTTTTTCTCTCCTTTCATGTAACATGCAATGGTCTTTCACACACCGTTATTTTGAACTCCTCGAATGAACCGTTTACCCGATCCTTCCAAAACAAAGCCATTTCGATCTTTGTGACTAAAACTCGAAGTGACCGTGATGTAAAAATTTACAGGGGCTGACCGAAAAATTCAAGGAAAAGGACACCATGTACTCTGGTTCAGCAACCTTCAGCTACGAGGCTTTCATGTTGACAGTCCTACCATTTCTCATTGCCTGAGGAGTTCTTCAGCCTTCGTTCCATCAGGTACGTTCATTTGGCTTTTCCTTCTTATTTCCATTGTGTTTGATAGAATATATTTATGCAGCCTTATCCTTATCCTATCCCATATTTCCAATTTCTCGTTTGTTTGTAAAATTTAGGTCATTGTGTCATATATGATGTAATGTGCAACCATTCATTGGGCTAA

mRNA sequence

ATGTCAGGTTACCGAAATCAACCTTCCGGTTACGGTTATGGTTACGGCGGTGGCGGTGGCGGTGGTGGTGGTGGTTACGGAGCTCCACCGCCGACGTCTCAGCCGTACAGTTCTCCACCCTACGGCTCGCAACATCCTCCGCCACAGTCACAGCAGCCGTACGCTCCGGTGGCCGCGCAATATGGCGCACCGTCGGCACCGTATGGATCATCTCCGGGCGAAAAACCGCCGAAGGACAAGCCTAATCAGTCTTACGGCGGATCAGCCAGCGGTGGTCATGGTTATCCACCGCCTTCTGCGTATGGAAGCCCATTCGCCTCACTCATGCCCTCCGCCTTTCCGCCTGGGACTGATCCGAGCATCGTTGCGTGTTTTCAAGTAGCCGATCGGGATAGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATGAGAACTGTTCACCTCCTCATGTTTCATTTCACCAACACAAGCACCAGGAAGATCGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCTAAGCTGGAGGGGTATTTTTGAGAGATTTGATTCAGACCGAAGTGGAAAAATTGATTCCAGAGAGTTGAAAGAAGCACTTTTCAGTTTGGGCTTTTCTGTCTCACCAATGATCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAATAGCAAGGCCATTGAGTATGATAATTTTATAGAGTGTTGTCTTACAGTCAAGGGGCTGACCGAAAAATTCAAGGAAAAGGACACCATGTACTCTGCCTTCGTTCCATCAGGTCATTGTGTCATATATGATGTAATGTGCAACCATTCATTGGGCTAA

Coding sequence (CDS)

ATGTCAGGTTACCGAAATCAACCTTCCGGTTACGGTTATGGTTACGGCGGTGGCGGTGGCGGTGGTGGTGGTGGTTACGGAGCTCCACCGCCGACGTCTCAGCCGTACAGTTCTCCACCCTACGGCTCGCAACATCCTCCGCCACAGTCACAGCAGCCGTACGCTCCGGTGGCCGCGCAATATGGCGCACCGTCGGCACCGTATGGATCATCTCCGGGCGAAAAACCGCCGAAGGACAAGCCTAATCAGTCTTACGGCGGATCAGCCAGCGGTGGTCATGGTTATCCACCGCCTTCTGCGTATGGAAGCCCATTCGCCTCACTCATGCCCTCCGCCTTTCCGCCTGGGACTGATCCGAGCATCGTTGCGTGTTTTCAAGTAGCCGATCGGGATAGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATGAGAACTGTTCACCTCCTCATGTTTCATTTCACCAACACAAGCACCAGGAAGATCGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCTAAGCTGGAGGGGTATTTTTGAGAGATTTGATTCAGACCGAAGTGGAAAAATTGATTCCAGAGAGTTGAAAGAAGCACTTTTCAGTTTGGGCTTTTCTGTCTCACCAATGATCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAATAGCAAGGCCATTGAGTATGATAATTTTATAGAGTGTTGTCTTACAGTCAAGGGGCTGACCGAAAAATTCAAGGAAAAGGACACCATGTACTCTGCCTTCGTTCCATCAGGTCATTGTGTCATATATGATGTAATGTGCAACCATTCATTGGGCTAA

Protein sequence

MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIECCLTVKGLTEKFKEKDTMYSAFVPSGHCVIYDVMCNHSLG
Homology
BLAST of Csor.00g155610.m01 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.4e-84
Identity = 189/327 (57.80%), Postives = 212/327 (64.83%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSS-----PPY---GSQHPPPQSQQ 60
           MSGY   PS  GYGYGG           PPP   PY S     PPY   GS  PPP    
Sbjct: 1   MSGY--PPSSQGYGYGGN----------PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSS 60

Query: 61  PYAPVAAQYGA--------PSAPYGSSPGE--KPPKDKPNQS------------------ 120
             +P A  YGA        PSAPY S PG+  KP K+KP+ +                  
Sbjct: 61  ASSPYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGP 120

Query: 121 ------------------YGGSA-----SGGHGYPPPSA-YGSPFASLMPSAFPPGTDPS 180
                             YGG+       GG+G PPP A YGSPFASL+PSAFPPGTDP+
Sbjct: 121 SDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPN 180

Query: 181 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 240
           IVACFQ ADRD SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIGPKEF +L
Sbjct: 181 IVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSL 240

Query: 241 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 268
           F+ L +WR IFERFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AI
Sbjct: 241 FFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAI 300

BLAST of Csor.00g155610.m01 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 5.9e-83
Identity = 191/342 (55.85%), Postives = 213/342 (62.28%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSS----PPYGSQ-----------H 60
           MSGY     GYGYGYGGG          PPP   PYSS    PPYGS             
Sbjct: 1   MSGYPPTSQGYGYGYGGG--------NQPPPPQPPYSSGGNNPPYGSSTTSSPYAVPYGA 60

Query: 61  PPPQSQQPYAPV--AAQYGA--PSAPYGSSPGE--KPPKDKP------------------ 120
             PQS    AP   ++ YGA  PSAPY  SPG+  KPPK+KP                  
Sbjct: 61  SKPQSSSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSSDYGS 120

Query: 121 ---------------------------NQSYGGS----ASGGH-----GYPPPSAYGSPF 180
                                        SYGG+    AS GH     GYPP ++YGSPF
Sbjct: 121 YGAGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPF 180

Query: 181 ASLMPSAFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFT 240
           ASL+PS F PGTDP+IVACFQ AD+D SGFIDDKELQG LSSY Q+FSMRTVHLLM+ FT
Sbjct: 181 ASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFT 240

Query: 241 NTSTRKIGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDL 268
           N++  KIGPKEF ALFY L +WR IFER D DRSG+ID  EL++AL SLGFSVSP++LDL
Sbjct: 241 NSNAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDL 300

BLAST of Csor.00g155610.m01 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 3.3e-81
Identity = 174/278 (62.59%), Postives = 199/278 (71.58%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPP---TSQPYSSPPYGSQHP----PPQSQQP 60
           M+GY   P G GY Y     GG GGYGAPPP   +S   S+PPYG++ P       S  P
Sbjct: 1   MAGYPPNP-GSGYPY-----GGAGGYGAPPPPYGSSPAPSAPPYGAKPPKEGKTSSSSAP 60

Query: 61  YAPVAAQYGAP--SAPYGSSPG-EKPPKDKP-NQSYGGSASGGHGYPPPSAYGSPFASLM 120
           Y      YGAP  + PYGS  G   PP  +P    YG        Y  P  YGSPFASL+
Sbjct: 61  YYGGGGGYGAPPSTQPYGSGGGYGAPPSSQPYGAPYGAPPPSSAPYGAPGGYGSPFASLV 120

Query: 121 PSAFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTST 180
           PSAFPPGTDP++VACFQ ADRD SG IDDKELQ  LS Y+Q FS+RTVHLLM+ FTNT+ 
Sbjct: 121 PSAFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSQSFSLRTVHLLMYLFTNTNV 180

Query: 181 RKIGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSK 240
           RKIGPKEF ++FY L +WR IFERFD DRSGKID+ EL++AL SLG+SVSP +LDLLVSK
Sbjct: 181 RKIGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDATELRDALLSLGYSVSPTVLDLLVSK 240

Query: 241 FDKSGGNSKAIEYDNFIECCLTVKGLTEKFKEKDTMYS 268
           FDK+GG +KAIEYDNFIECCLTVKGLTEKFKEKDT +S
Sbjct: 241 FDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAFS 272

BLAST of Csor.00g155610.m01 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 1.3e-29
Identity = 81/212 (38.21%), Postives = 125/212 (58.96%), Query Frame = 0

Query: 63  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYP--PPSAYGSPFASLMPSAFPPGTDPS 122
           APSAP       + P+    Q + G +   + YP   P+   S ++ +    F P T P 
Sbjct: 8   APSAP-------ELPESFVQQQHDGESRYTYAYPSYQPTQQFSSYSGM----FSPETHPE 67

Query: 123 IVACFQVADRDRSGFIDDKELQGVLS-SYNQKFSMRTVHLLMFHF--TNTSTRKIGPKEF 182
           IV  F+ ADR+RSGF+++ EL+  LS S     S RT+ LL+F +     S  ++GPKE+
Sbjct: 68  IVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLGPKEY 127

Query: 183 IALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNS 242
           + L+  L  WR IF R+D DRSGK++S +L++A ++LG  +   +  L+VS+FD   G +
Sbjct: 128 VELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDGTGKT 187

Query: 243 KAIEYDNFIECCLTVKGLTEKFKEKDTMYSAF 270
             + +D+F+EC + VKGLTEKF+E D  Y+ +
Sbjct: 188 VDLCFDSFLECGMIVKGLTEKFRENDPGYTGY 208

BLAST of Csor.00g155610.m01 vs. ExPASy Swiss-Prot
Match: Q9UBV8 (Peflin OS=Homo sapiens OX=9606 GN=PEF1 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 6.7e-26
Identity = 95/256 (37.11%), Postives = 132/256 (51.56%), Query Frame = 0

Query: 17  GGGGGGGGGYGAPPPT---SQPYSSPPYGSQHPPPQSQQPYAPVAAQYGAPS--APYGSS 76
           G  G  G   GAPP +     P S   YGS  PP       AP    YG P+   PYG  
Sbjct: 9   GCPGAAGQAPGAPPGSYYPGPPNSGGQYGSGLPPGGGYGGPAP-GGPYGPPAGGGPYGHP 68

Query: 77  PGEKPPKDKPNQSYGGSASGG-HGYPPPSAYGSPFASLM-PSAFPPGTDPSIVACFQVAD 136
                P   P   YGG+A GG +G PPPS+YG+    L      PP  DP   + FQ  D
Sbjct: 69  NPGMFPSGTPGGPYGGAAPGGPYGQPPPSSYGAQQPGLYGQGGAPPNVDPEAYSWFQSVD 128

Query: 137 RDRSGFIDDKELQGVLSSYN-QKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGLLSWR 196
            D SG+I  KEL+  L + N   F+  T  +++  F  T + +I    F AL+  +  W+
Sbjct: 129 SDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALWKFIQQWK 188

Query: 197 GIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIEC 256
            +F+++D DRSG I   EL++AL  +G+++SP    LLVS++     N  A++ D FI+ 
Sbjct: 189 NLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSAN-PAMQLDRFIQV 248

Query: 257 CLTVKGLTEKFKEKDT 265
           C  ++ LTE F+EKDT
Sbjct: 249 CTQLQVLTEAFREKDT 262

BLAST of Csor.00g155610.m01 vs. NCBI nr
Match: KAG6603192.1 (putative calcium-binding protein CML49, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 572 bits (1473), Expect = 1.51e-205
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ
Sbjct: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS
Sbjct: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL
Sbjct: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI
Sbjct: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYSAFVPSGHCVIYDVMCNHSLG 287
           EYDNFIECCLTVKGLTEKFKEKDTMYSAFVPSGHCVIYDVMCNHSLG
Sbjct: 241 EYDNFIECCLTVKGLTEKFKEKDTMYSAFVPSGHCVIYDVMCNHSLG 287

BLAST of Csor.00g155610.m01 vs. NCBI nr
Match: KAG7033501.1 (putative calcium-binding protein CML49, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 527 bits (1358), Expect = 5.11e-188
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ
Sbjct: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS
Sbjct: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL
Sbjct: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI
Sbjct: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 267
           EYDNFIECCLTVKGLTEKFKEKDTMYS
Sbjct: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 267

BLAST of Csor.00g155610.m01 vs. NCBI nr
Match: XP_023544753.1 (probable calcium-binding protein CML49 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521 bits (1343), Expect = 9.51e-186
Identity = 266/267 (99.63%), Postives = 266/267 (99.63%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGYRNQPSGYGYGYGGGGGGG GGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ
Sbjct: 1   MSGYRNQPSGYGYGYGGGGGGG-GGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS
Sbjct: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL
Sbjct: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI
Sbjct: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 267
           EYDNFIECCLTVKGLTEKFKEKDTMYS
Sbjct: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 266

BLAST of Csor.00g155610.m01 vs. NCBI nr
Match: XP_022928660.1 (probable calcium-binding protein CML49 [Cucurbita moschata])

HSP 1 Score: 520 bits (1339), Expect = 3.73e-185
Identity = 265/267 (99.25%), Postives = 265/267 (99.25%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGYRNQPSGYGYGYGGGGGG  GGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ
Sbjct: 1   MSGYRNQPSGYGYGYGGGGGG--GGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS
Sbjct: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL
Sbjct: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI
Sbjct: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 267
           EYDNFIECCLTVKGLTEKFKEKDTMYS
Sbjct: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 265

BLAST of Csor.00g155610.m01 vs. NCBI nr
Match: XP_022967874.1 (probable calcium-binding protein CML50 [Cucurbita maxima])

HSP 1 Score: 510 bits (1313), Expect = 3.06e-181
Identity = 260/267 (97.38%), Postives = 260/267 (97.38%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGYRNQPSGYGY Y     GGGGGYGAPPPTSQPYSSPPYGSQHPP QSQQPYAPVAAQ
Sbjct: 1   MSGYRNQPSGYGYSY-----GGGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQ 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS
Sbjct: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL
Sbjct: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI
Sbjct: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 267
           EYDNFIECCLTVKGLTEKFKEKDTMYS
Sbjct: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 262

BLAST of Csor.00g155610.m01 vs. ExPASy TrEMBL
Match: A0A6J1ES88 (probable calcium-binding protein CML49 OS=Cucurbita moschata OX=3662 GN=LOC111435509 PE=4 SV=1)

HSP 1 Score: 520 bits (1339), Expect = 1.81e-185
Identity = 265/267 (99.25%), Postives = 265/267 (99.25%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGYRNQPSGYGYGYGGGGGG  GGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ
Sbjct: 1   MSGYRNQPSGYGYGYGGGGGG--GGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS
Sbjct: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL
Sbjct: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI
Sbjct: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 267
           EYDNFIECCLTVKGLTEKFKEKDTMYS
Sbjct: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 265

BLAST of Csor.00g155610.m01 vs. ExPASy TrEMBL
Match: A0A6J1HXZ3 (probable calcium-binding protein CML50 OS=Cucurbita maxima OX=3661 GN=LOC111467251 PE=4 SV=1)

HSP 1 Score: 510 bits (1313), Expect = 1.48e-181
Identity = 260/267 (97.38%), Postives = 260/267 (97.38%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGYRNQPSGYGY Y     GGGGGYGAPPPTSQPYSSPPYGSQHPP QSQQPYAPVAAQ
Sbjct: 1   MSGYRNQPSGYGYSY-----GGGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQ 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS
Sbjct: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL
Sbjct: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI
Sbjct: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 267
           EYDNFIECCLTVKGLTEKFKEKDTMYS
Sbjct: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 262

BLAST of Csor.00g155610.m01 vs. ExPASy TrEMBL
Match: A0A6J1HP98 (probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC111466073 PE=4 SV=1)

HSP 1 Score: 456 bits (1172), Expect = 5.37e-160
Identity = 233/272 (85.66%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGY NQPSGYGYGY     GG GGYGAPPPT+QPY S PYGSQHPPP SQQPYAPVAA 
Sbjct: 1   MSGYPNQPSGYGYGY-----GGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAP 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGG-----SASGGHGYPPPSAYGSPFASLMPSAFPP 120
           YGAPSAPYGS+PGEKP KDKPNQS+GG      + GGHGYPPPSAYGSPFASL+PSAFPP
Sbjct: 61  YGAPSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPP 120

Query: 121 GTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPK 180
           GTDPSIV CFQVAD+D SGFIDDKELQGVLSSYNQKFS+RTVHLLM+HFTNT+TRKIGPK
Sbjct: 121 GTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPK 180

Query: 181 EFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGG 240
           EFIALFYGL SWRGIFERFDSDRSGKID  EL+EAL SLGF+VSPM+LDLLVSKFDKSGG
Sbjct: 181 EFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGG 240

Query: 241 NSKAIEYDNFIECCLTVKGLTEKFKEKDTMYS 267
            SKAIEYDNFIECCLTVKGLTEKFKEKDT YS
Sbjct: 241 KSKAIEYDNFIECCLTVKGLTEKFKEKDTTYS 267

BLAST of Csor.00g155610.m01 vs. ExPASy TrEMBL
Match: A0A6J1EBH1 (probable calcium-binding protein CML49 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432514 PE=4 SV=1)

HSP 1 Score: 453 bits (1166), Expect = 4.40e-159
Identity = 232/272 (85.29%), Postives = 244/272 (89.71%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGY NQPSGYGYGY     GG GGYGAPPPT+QPY S PYGSQHPPP SQQPYAPVAA 
Sbjct: 1   MSGYPNQPSGYGYGY-----GGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAP 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGG-----SASGGHGYPPPSAYGSPFASLMPSAFPP 120
           YGAPSAPYGS+P EKP KDKPNQS+GG      + GGHGYPPPSAYGSPFASL+PSAFPP
Sbjct: 61  YGAPSAPYGSNPSEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPP 120

Query: 121 GTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPK 180
           GTDPSIV CFQVAD+D SGFIDDKELQGVLSSYNQ+FS+RTVHLLM+HFTNT+TRKIGPK
Sbjct: 121 GTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQRFSIRTVHLLMYHFTNTNTRKIGPK 180

Query: 181 EFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGG 240
           EFIALFYGL SWRGIFERFDSDRSGKID  EL+EAL SLGFSVSPM+LDLLVSKFDKSGG
Sbjct: 181 EFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFSVSPMVLDLLVSKFDKSGG 240

Query: 241 NSKAIEYDNFIECCLTVKGLTEKFKEKDTMYS 267
            SKAIEYDNFIECCLTVKGLTEKFKEKDT YS
Sbjct: 241 KSKAIEYDNFIECCLTVKGLTEKFKEKDTTYS 267

BLAST of Csor.00g155610.m01 vs. ExPASy TrEMBL
Match: A0A6J1DIS2 (probable calcium-binding protein CML49 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021303 PE=4 SV=1)

HSP 1 Score: 447 bits (1149), Expect = 2.44e-156
Identity = 230/277 (83.03%), Postives = 248/277 (89.53%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGG-GGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAA 60
           MSGY NQPSGYGYGYGG G GGG GGYGAPPPT+QPY+S  YG Q PPP SQQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60

Query: 61  QYGAPSAPYGSSPGEKPPKDKPNQSYGGS---------ASGGHGYPPPSAYGSPFASLMP 120
            YGAPSAPYGSS G+KPPKDKP+QSYGG+         A GGH YPPP++YG+PFASL+P
Sbjct: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTR 180
           SAFPPGTDPSIVACFQVAD+D SGFIDDKELQGVLSSYNQKFS+RTVHLLM+HFTNT+TR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKF 240
           KIGPKEFIALFYGL SWRGIFERFDSDRSGKIDS EL+EAL SLGF+VSP++LDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240

Query: 241 DKSGGNSKAIEYDNFIECCLTVKGLTEKFKEKDTMYS 267
           DKSGG SKAIEYDNFIECCLTVKGLTEKFKEKDT YS
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYS 277

BLAST of Csor.00g155610.m01 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 313.5 bits (802), Expect = 1.7e-85
Identity = 189/327 (57.80%), Postives = 212/327 (64.83%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSS-----PPY---GSQHPPPQSQQ 60
           MSGY   PS  GYGYGG           PPP   PY S     PPY   GS  PPP    
Sbjct: 1   MSGY--PPSSQGYGYGGN----------PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSS 60

Query: 61  PYAPVAAQYGA--------PSAPYGSSPGE--KPPKDKPNQS------------------ 120
             +P A  YGA        PSAPY S PG+  KP K+KP+ +                  
Sbjct: 61  ASSPYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGP 120

Query: 121 ------------------YGGSA-----SGGHGYPPPSA-YGSPFASLMPSAFPPGTDPS 180
                             YGG+       GG+G PPP A YGSPFASL+PSAFPPGTDP+
Sbjct: 121 SDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPN 180

Query: 181 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 240
           IVACFQ ADRD SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIGPKEF +L
Sbjct: 181 IVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSL 240

Query: 241 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 268
           F+ L +WR IFERFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AI
Sbjct: 241 FFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAI 300

BLAST of Csor.00g155610.m01 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 308.9 bits (790), Expect = 4.2e-84
Identity = 191/342 (55.85%), Postives = 213/342 (62.28%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSS----PPYGSQ-----------H 60
           MSGY     GYGYGYGGG          PPP   PYSS    PPYGS             
Sbjct: 1   MSGYPPTSQGYGYGYGGG--------NQPPPPQPPYSSGGNNPPYGSSTTSSPYAVPYGA 60

Query: 61  PPPQSQQPYAPV--AAQYGA--PSAPYGSSPGE--KPPKDKP------------------ 120
             PQS    AP   ++ YGA  PSAPY  SPG+  KPPK+KP                  
Sbjct: 61  SKPQSSSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSSDYGS 120

Query: 121 ---------------------------NQSYGGS----ASGGH-----GYPPPSAYGSPF 180
                                        SYGG+    AS GH     GYPP ++YGSPF
Sbjct: 121 YGAGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPF 180

Query: 181 ASLMPSAFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFT 240
           ASL+PS F PGTDP+IVACFQ AD+D SGFIDDKELQG LSSY Q+FSMRTVHLLM+ FT
Sbjct: 181 ASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFT 240

Query: 241 NTSTRKIGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDL 268
           N++  KIGPKEF ALFY L +WR IFER D DRSG+ID  EL++AL SLGFSVSP++LDL
Sbjct: 241 NSNAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDL 300

BLAST of Csor.00g155610.m01 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.0e-74
Identity = 171/307 (55.70%), Postives = 194/307 (63.19%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSS-----PPY---GSQHPPPQSQQ 60
           MSGY   PS  GYGYGG           PPP   PY S     PPY   GS  PPP    
Sbjct: 1   MSGY--PPSSQGYGYGGN----------PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSS 60

Query: 61  PYAPVAAQYGA--------PSAPYGSSPGE--KPPKDKPNQS------------------ 120
             +P A  YGA        PSAPY S PG+  KP K+KP+ +                  
Sbjct: 61  ASSPYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGP 120

Query: 121 ------------------YGGSA-----SGGHGYPPPSA-YGSPFASLMPSAFPPGTDPS 180
                             YGG+       GG+G PPP A YGSPFASL+PSAFPPGTDP+
Sbjct: 121 SDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPN 180

Query: 181 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 240
           IVACFQ ADRD SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIGPKEF +L
Sbjct: 181 IVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSL 240

Query: 241 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 248
           F+ L +WR IFERFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AI
Sbjct: 241 FFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAI 295

BLAST of Csor.00g155610.m01 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.0e-74
Identity = 171/307 (55.70%), Postives = 194/307 (63.19%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSS-----PPY---GSQHPPPQSQQ 60
           MSGY   PS  GYGYGG           PPP   PY S     PPY   GS  PPP    
Sbjct: 1   MSGY--PPSSQGYGYGGN----------PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSS 60

Query: 61  PYAPVAAQYGA--------PSAPYGSSPGE--KPPKDKPNQS------------------ 120
             +P A  YGA        PSAPY S PG+  KP K+KP+ +                  
Sbjct: 61  ASSPYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGP 120

Query: 121 ------------------YGGSA-----SGGHGYPPPSA-YGSPFASLMPSAFPPGTDPS 180
                             YGG+       GG+G PPP A YGSPFASL+PSAFPPGTDP+
Sbjct: 121 SDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPN 180

Query: 181 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 240
           IVACFQ ADRD SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIGPKEF +L
Sbjct: 181 IVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSL 240

Query: 241 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 248
           F+ L +WR IFERFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AI
Sbjct: 241 FFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAI 295

BLAST of Csor.00g155610.m01 vs. TAIR 10
Match: AT3G10300.5 (Calcium-binding EF-hand family protein )

HSP 1 Score: 271.6 bits (693), Expect = 7.5e-73
Identity = 171/308 (55.52%), Postives = 194/308 (62.99%), Query Frame = 0

Query: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSS-----PPY---GSQHPPPQSQQ 60
           MSGY   PS  GYGYGG           PPP   PY S     PPY   GS  PPP    
Sbjct: 1   MSGY--PPSSQGYGYGGN----------PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSS 60

Query: 61  PYAPVAAQYGA--------PSAPYGSSPGE--KPPKDKPNQS------------------ 120
             +P A  YGA        PSAPY S PG+  KP K+KP+ +                  
Sbjct: 61  ASSPYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGP 120

Query: 121 ------------------YGGSA-----SGGHGYPPPSA-YGSPFASLMPSAFPPGTDPS 180
                             YGG+       GG+G PPP A YGSPFASL+PSAFPPGTDP+
Sbjct: 121 SDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPN 180

Query: 181 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIG-PKEFIA 240
           IVACFQ ADRD SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIG PKEF +
Sbjct: 181 IVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGRPKEFTS 240

Query: 241 LFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKA 248
           LF+ L +WR IFERFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++A
Sbjct: 241 LFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRA 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W4L02.4e-8457.80Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ... [more]
Q9FYE45.9e-8355.85Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ... [more]
Q2QY103.3e-8162.59Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2... [more]
Q9ZQH11.3e-2938.21Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ... [more]
Q9UBV86.7e-2637.11Peflin OS=Homo sapiens OX=9606 GN=PEF1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6603192.11.51e-205100.00putative calcium-binding protein CML49, partial [Cucurbita argyrosperma subsp. s... [more]
KAG7033501.15.11e-188100.00putative calcium-binding protein CML49, partial [Cucurbita argyrosperma subsp. a... [more]
XP_023544753.19.51e-18699.63probable calcium-binding protein CML49 [Cucurbita pepo subsp. pepo][more]
XP_022928660.13.73e-18599.25probable calcium-binding protein CML49 [Cucurbita moschata][more]
XP_022967874.13.06e-18197.38probable calcium-binding protein CML50 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1ES881.81e-18599.25probable calcium-binding protein CML49 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1HXZ31.48e-18197.38probable calcium-binding protein CML50 OS=Cucurbita maxima OX=3661 GN=LOC1114672... [more]
A0A6J1HP985.37e-16085.66probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC1114660... [more]
A0A6J1EBH14.40e-15985.29probable calcium-binding protein CML49 isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1DIS22.44e-15683.03probable calcium-binding protein CML49 isoform X1 OS=Momordica charantia OX=3673... [more]
Match NameE-valueIdentityDescription
AT3G10300.31.7e-8557.80Calcium-binding EF-hand family protein [more]
AT5G04170.14.2e-8455.85Calcium-binding EF-hand family protein [more]
AT3G10300.23.0e-7455.70Calcium-binding EF-hand family protein [more]
AT3G10300.43.0e-7455.70Calcium-binding EF-hand family protein [more]
AT3G10300.57.5e-7355.52Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 120..148
e-value: 0.19
score: 20.8
coord: 186..214
e-value: 0.012
score: 24.8
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 121..144
e-value: 8.1E-5
score: 21.9
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 187..215
e-value: 2.2E-5
score: 23.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 116..151
score: 10.384886
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 182..217
score: 11.75176
NoneNo IPR availableGENE3D1.10.238.10coord: 103..270
e-value: 1.4E-35
score: 124.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..53
NoneNo IPR availablePANTHERPTHR46824:SF1CALCIUM-BINDING PROTEIN CML49-RELATEDcoord: 1..268
NoneNo IPR availableCDDcd16180EFh_PEF_Group_Icoord: 120..270
e-value: 5.69698E-64
score: 196.595
IPR044590Probable calcium-binding protein CML48/49/50PANTHERPTHR46824CALCIUM-BINDING PROTEIN CML48-RELATEDcoord: 1..268
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 129..141
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 195..207
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 114..270

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Csor.00g155610Csor.00g155610gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g155610.m01.CDS07Csor.00g155610.m01.CDS07CDS
Csor.00g155610.m01.CDS06Csor.00g155610.m01.CDS06CDS
Csor.00g155610.m01.CDS05Csor.00g155610.m01.CDS05CDS
Csor.00g155610.m01.CDS04Csor.00g155610.m01.CDS04CDS
Csor.00g155610.m01.CDS03Csor.00g155610.m01.CDS03CDS
Csor.00g155610.m01.CDS02Csor.00g155610.m01.CDS02CDS
Csor.00g155610.m01.CDS01Csor.00g155610.m01.CDS01CDS


The following terminal feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g155610.m01.terminal01Csor.00g155610.m01.terminal01terminal


The following stop_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g155610.m01.stop_codon01Csor.00g155610.m01.stop_codon01stop_codon


The following intron feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g155610.m01.intron06Csor.00g155610.m01.intron06intron
Csor.00g155610.m01.intron05Csor.00g155610.m01.intron05intron
Csor.00g155610.m01.intron04Csor.00g155610.m01.intron04intron
Csor.00g155610.m01.intron03Csor.00g155610.m01.intron03intron
Csor.00g155610.m01.intron02Csor.00g155610.m01.intron02intron
Csor.00g155610.m01.intron01Csor.00g155610.m01.intron01intron


The following internal feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g155610.m01.internal05Csor.00g155610.m01.internal05internal
Csor.00g155610.m01.internal04Csor.00g155610.m01.internal04internal
Csor.00g155610.m01.internal03Csor.00g155610.m01.internal03internal
Csor.00g155610.m01.internal02Csor.00g155610.m01.internal02internal
Csor.00g155610.m01.internal01Csor.00g155610.m01.internal01internal


The following initial feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g155610.m01.initial01Csor.00g155610.m01.initial01initial


The following start_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g155610.m01.start_codon01Csor.00g155610.m01.start_codon01start_codon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Csor.00g155610.m01Csor.00g155610.m01-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding