Csor.00g066680.m01 (mRNA) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g066680.m01
Type	mRNA
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	aspartic proteinase CDR1-like
Location	Csor_Chr04: 12012445 .. 12013803 (+)
Sequence length	1200
RNA-Seq Expression	Csor.00g066680.m01
Synteny	Csor.00g066680.m01

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAATCCCATGGCGCCTACCATATTTATCGTTCTCGCACTACTTTCCATCGCCGAGTCCACCGTCGGCAAAGGCGGTGGTCTCAAGCTGGAACTCATCCAACGCCGACTCTCACCAGGCAACGTTTCACCGATGGCAGCCAAATCACAAATTTGGCCGGAAACCAGCGAATTTATAGTGAAAATCGCCGTCGGAACGCCGCCGACGGAGGTGCATGCAATCCTCGACACTGGCAGCGATTTATTTTGGGCTCAGTGTCGTCCATGTGCAAAATGTTACCGGCAAACGAATCGATTTACGACCCTTCGAAATCGTCAACCTTTCGAACCCTTTCTTGCAAGTCGCCGCAGTGCCATTTGAGGGGGTCCGGTGCGGCGTGCTCCGGCACCGACACGTGTAAGTACGGCTATGGGTATGGAAGCGGATCTACGCAGGGAGAATTGGCGACTGAAAAAATGGCTGTAACTTCGAGGTCTGGAGCGACGACGCCGTTTTCGGGGGTGGTGTTTGGTTGCGGACATAATAATAGTGGAACGTTTAATGCCAATGAAATGGGATTGATCGGATTTGGAAGAGGAGCGATTTCCTTCGTTTCTCAGGTATATATATATATATATATATATTATCACTTTTAATTATTATTAATATTTTTAAAAACATTATTAGCATTAAACAGTGAAACCAACCCTTAATTATATTCTTATTCAAATAACTATAAAGTTCAAAACCTTATAGTTAACGTAAATTCATATTCACAGATAGGTCCATCGGTCGGCGGCAGAAAGTTCTCCCTTTGTCTGATGCCATACAACACCGACCCGAGAATCTCAAGTAGTCTCTCTATCGGGTCGGGTTCTGAAGTTAAAGGACCCGGAGTCATCACAGCCCAACTCGTTCGAACACCCGACCAGACATCTTACTCTCTCACTCTCACGGGAATCTCCGTTGGAAAAACCCTTGTTCCGTACAGTATGTCGGGACCTCCGGCCAAGGGGAACGCGGTTCTCGATACCGGCACGCCGCCGACTCTCCTCCCCAAAGAATTGTACGGACGATTGGCTGCCGAAGTTCGGCGGCATATCCCGTCGAAGCCCGTTGACGATGATACCCTTTGCTACAAAGATAATTTGGGGGATTTGGTTATGACTCTACACTTCGAGGGCGGCGTGGATCTGCGATTGAGTACGGTTCAGACTTTCAATAAGATGTCGGATGGGTCCTTTTGCTTCACCGCGATGGGCGTTGACGACAAGGACGCACTCATCGGGAACAGTATGATGGCGAATTTTTTGGTTGGGTATGATATTGACAATATGACGGTGTCGTTTAAGCCCACTGATTGCACAAAAATTGGTTGA

mRNA sequence

ATGCAATCCCATGGCGCCTACCATATTTATCGTTCTCGCACTACTTTCCATCGCCGAGTCCACCGTCGGCAAAGGCGGTGGTCTCAAGCTGGAACTCATCCAACGCCGACTCTCACCAGGCAACGTTTCACCGATGGCAGCCAAATCACAAATTTGGCCGGAAACCAGCGAATTTATAGTGAAAATCGCCGTCGGAACGCCGCCGACGGAGGTGCATGCAATCCTCGACACTGGCAGCGATTTATTTTGGGCTCAGTGTCGTCCATGTGCAAAATGTTACCGGCAAACGAATCGATTTACGACCCTTCGAAATCGTCAACCTTTCGAACCCTTTCTTGCAAGTCGCCGCAGTGCCATTTGAGGGGGTCCGGTGCGGCGTGCTCCGGCACCGACACGTGTAAGTACGGCTATGGGTATGGAAGCGGATCTACGCAGGGAGAATTGGCGACTGAAAAAATGGCTGTAACTTCGAGGTCTGGAGCGACGACGCCGTTTTCGGGGGTGGTGTTTGGTTGCGGACATAATAATAGTGGAACGTTTAATGCCAATGAAATGGGATTGATCGGATTTGGAAGAGGAGCGATTTCCTTCGTTTCTCAGATAGGTCCATCGGTCGGCGGCAGAAAGTTCTCCCTTTGTCTGATGCCATACAACACCGACCCGAGAATCTCAAGTAGTCTCTCTATCGGGTCGGGTTCTGAAGTTAAAGGACCCGGAGTCATCACAGCCCAACTCGTTCGAACACCCGACCAGACATCTTACTCTCTCACTCTCACGGGAATCTCCGTTGGAAAAACCCTTGTTCCGTACAGTATGTCGGGACCTCCGGCCAAGGGGAACGCGGTTCTCGATACCGGCACGCCGCCGACTCTCCTCCCCAAAGAATTGTACGGACGATTGGCTGCCGAAGTTCGGCGGCATATCCCGTCGAAGCCCGTTGACGATGATACCCTTTGCTACAAAGATAATTTGGGGGATTTGGTTATGACTCTACACTTCGAGGGCGGCGTGGATCTGCGATTGAGTACGGTTCAGACTTTCAATAAGATGTCGGATGGGTCCTTTTGCTTCACCGCGATGGGCGTTGACGACAAGGACGCACTCATCGGGAACAGTATGATGGCGAATTTTTTGGTTGGGTATGATATTGACAATATGACGGTGTCGTTTAAGCCCACTGATTGCACAAAAATTGGTTGA

Coding sequence (CDS)

ATGCAATCCCATGGCGCCTACCATATTTATCGTTCTCGCACTACTTTCCATCGCCGAGTCCACCGTCGGCAAAGGCGGTGGTCTCAAGCTGGAACTCATCCAACGCCGACTCTCACCAGGCAACGTTTCACCGATGGCAGCCAAATCACAAATTTGGCCGGAAACCAGCGAATTTATAGTGAAAATCGCCGTCGGAACGCCGCCGACGGAGGTGCATGCAATCCTCGACACTGGCAGCGATTTATTTTGGGCTCAGTGTCGTCCATGTGCAAAATGTTACCGGCAAACGAATCGATTTACGACCCTTCGAAATCGTCAACCTTTCGAACCCTTTCTTGCAAGTCGCCGCAGTGCCATTTGAGGGGGTCCGGTGCGGCGTGCTCCGGCACCGACACGTGTAAGTACGGCTATGGGTATGGAAGCGGATCTACGCAGGGAGAATTGGCGACTGAAAAAATGGCTGTAACTTCGAGGTCTGGAGCGACGACGCCGTTTTCGGGGGTGGTGTTTGGTTGCGGACATAATAATAGTGGAACGTTTAATGCCAATGAAATGGGATTGATCGGATTTGGAAGAGGAGCGATTTCCTTCGTTTCTCAGATAGGTCCATCGGTCGGCGGCAGAAAGTTCTCCCTTTGTCTGATGCCATACAACACCGACCCGAGAATCTCAAGTAGTCTCTCTATCGGGTCGGGTTCTGAAGTTAAAGGACCCGGAGTCATCACAGCCCAACTCGTTCGAACACCCGACCAGACATCTTACTCTCTCACTCTCACGGGAATCTCCGTTGGAAAAACCCTTGTTCCGTACAGTATGTCGGGACCTCCGGCCAAGGGGAACGCGGTTCTCGATACCGGCACGCCGCCGACTCTCCTCCCCAAAGAATTGTACGGACGATTGGCTGCCGAAGTTCGGCGGCATATCCCGTCGAAGCCCGTTGACGATGATACCCTTTGCTACAAAGATAATTTGGGGGATTTGGTTATGACTCTACACTTCGAGGGCGGCGTGGATCTGCGATTGAGTACGGTTCAGACTTTCAATAAGATGTCGGATGGGTCCTTTTGCTTCACCGCGATGGGCGTTGACGACAAGGACGCACTCATCGGGAACAGTATGATGGCGAATTTTTTGGTTGGGTATGATATTGACAATATGACGGTGTCGTTTAAGCCCACTGATTGCACAAAAATTGGTTGA

Protein sequence

MQSHGAYHIYRSRTTFHRRVHRRQRRWSQAGTHPTPTLTRQRFTDGSQITNLAGNQRIYSENRRRNAADGGACNPRHWQRFILGSVSSMCKMLPANESIYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSRSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYNTDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAKGNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKIG

Homology

[Close]

BLAST of Csor.00g066680.m01 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.1e-50
Identity = 120/313 (38.34%), Postives = 176/313 (56.23%), Query Frame = 0


Query: 97  ESIYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTD-TCKYGYGYGSGS-TQGELATEKMA 156
           + ++DP  SST++ +SC S QC    + A+CS  D TC Y   YG  S T+G +A + + 
Sbjct: 129 DPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLT 188

Query: 157 VTSRSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCL 216
           + S          ++ GCGHNN+GTFN    G++G G G +S + Q+G S+ G KFS CL
Sbjct: 189 LGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDG-KFSYCL 248

Query: 217 MPYNTDPRISSSLSIGSGSEVKGPGVITAQLV-RTPDQTSYSLTLTGISVGKTLVPYSMS 276
           +P  +    +S ++ G+ + V G GV++  L+ +   +T Y LTL  ISVG   + YS S
Sbjct: 249 VPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSGS 308

Query: 277 -GPPAKGNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDD----TLCYKDNLGDL- 336
               ++GN ++D+GT  TLLP E Y  L   V   I ++   D     +LCY    GDL 
Sbjct: 309 DSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSAT-GDLK 368

Query: 337 --VMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDN 396
             V+T+HF+ G D++L +   F ++S+   CF   G     ++ GN    NFLVGYD  +
Sbjct: 369 VPVITMHFD-GADVKLDSSNAFVQVSEDLVCFAFRG-SPSFSIYGNVAQMNFLVGYDTVS 428

Query: 397 MTVSFKPTDCTKI 399
            TVSFKPTDC K+
Sbjct: 429 KTVSFKPTDCAKM 437

[Close]

BLAST of Csor.00g066680.m01 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.4e-42
Identity = 115/325 (35.38%), Postives = 175/325 (53.85%), Query Frame = 0


Query: 96  NESIYDPSKSSTFRTLSCKSPQCH-LRGSGAAC-SGTDTCKYGYGYGSGS-TQGELATEK 155
           N  I+D  KSST+++  C S  C  L  +   C    + CKY Y YG  S ++G++ATE 
Sbjct: 123 NGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATET 182

Query: 156 MAVTSRSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSL 215
           +++ S SG+   F G VFGCG+NN GTF+    G+IG G G +S +SQ+G S+  +KFS 
Sbjct: 183 VSIDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLGSSI-SKKFSY 242

Query: 216 CLMPYNTDPRISSSLSIGS----GSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLV 275
           CL   +     +S +++G+     S  K  GV++  LV     T Y LTL  ISVGK  +
Sbjct: 243 CLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKKI 302

Query: 276 PYSMSG---------PPAKGNAVLDTGTPPTLLPKELYGRLAAEVRRHIP-SKPVDDD-- 335
           PY+ S              GN ++D+GT  TLL    + + ++ V   +  +K V D   
Sbjct: 303 PYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQG 362

Query: 336 --TLCYKDNLGDL---VMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGN 395
             + C+K    ++    +T+HF  G D+RLS +  F K+S+   C + +   +  A+ GN
Sbjct: 363 LLSHCFKSGSAEIGLPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMVPTTEV-AIYGN 422

Query: 396 SMMANFLVGYDIDNMTVSFKPTDCT 397
               +FLVGYD++  TVSF+  DC+
Sbjct: 423 FAQMDFLVGYDLETRTVSFQHMDCS 444

[Close]

BLAST of Csor.00g066680.m01 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.1e-29
Identity = 102/313 (32.59%), Postives = 143/313 (45.69%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGS-TQGELATEKMAVTS 158
           I++P  SS+F TL C S  C    S   CS  + C+Y YGYG GS TQG + TE +   S
Sbjct: 136 IFNPQGSSSFSTLPCSSQLCQAL-SSPTCS-NNFCQYTYGYGDGSETQGSMGTETLTFGS 195

Query: 159 RSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPY 218
            S        + FGCG NN G    N  GL+G GRG +S  SQ+  +    KFS C+ P 
Sbjct: 196 VS-----IPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVT----KFSYCMTPI 255

Query: 219 NTDPRISSSLSIGSGSEVKGPGVITAQLVRTPD-QTSYSLTLTGISVGKTLVP-----YS 278
            +     S+L +GS +     G     L+++    T Y +TL G+SVG T +P     ++
Sbjct: 256 GSS--TPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFA 315

Query: 279 MSGPPAKGNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDT----LCYK-----D 338
           ++     G  ++D+GT  T      Y  +  E    I    V+  +    LC++      
Sbjct: 316 LNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPS 375

Query: 339 NLGDLVMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYD 396
           NL      +HF+GG DL L +   F   S+G  C          ++ GN    N LV YD
Sbjct: 376 NLQIPTFVMHFDGG-DLELPSENYFISPSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYD 434

[Close]

BLAST of Csor.00g066680.m01 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 4.2e-26
Identity = 104/314 (33.12%), Postives = 153/314 (48.73%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGS-TQGELATEKMAVTS 158
           I++P  SS+F TL C+S  C    S   C+  + C+Y YGYG GS TQG +ATE      
Sbjct: 137 IFNPQDSSSFSTLPCESQYCQDLPS-ETCNNNE-CQYTYGYGDGSTTQGYMATETFTF-- 196

Query: 159 RSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPY 218
               T+    + FGCG +N G    N  GLIG G G +S  SQ+G  VG  +FS C+  Y
Sbjct: 197 ---ETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLG--VG--QFSYCMTSY 256

Query: 219 NTDPRISSSLSIGSGSEVKGPGVITAQLVRTP-DQTSYSLTLTGISVG--KTLVPYS--M 278
            +     S+L++GS +     G  +  L+ +  + T Y +TL GI+VG     +P S   
Sbjct: 257 GSSS--PSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQ 316

Query: 279 SGPPAKGNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDD----TLCYKD-NLGDL 338
                 G  ++D+GT  T LP++ Y  +A      I    VD+     + C++  + G  
Sbjct: 317 LQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGST 376

Query: 339 V----MTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAMGVDDK--DALIGNSMMANFLVGY 396
           V    +++ F+GGV L L         ++G  C  AMG   +   ++ GN       V Y
Sbjct: 377 VQVPEISMQFDGGV-LNLGEQNILISPAEGVICL-AMGSSSQLGISIFGNIQQQETQVLY 435

[Close]

BLAST of Csor.00g066680.m01 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 7.9e-25
Identity = 96/315 (30.48%), Postives = 136/315 (43.17%), Query Frame = 0


Query: 96  NESIYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGS-TQGELATEKMA 155
           ++ I+DP KS T+ T+ C SP C    S    +   TC Y   YG GS T G+ +TE + 
Sbjct: 180 SDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLT 239

Query: 156 VTSRSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCL 215
                       GV  GCGH+N G F     GL+G G+G +SF  Q G     +KFS CL
Sbjct: 240 FRRNR-----VKGVALGCGHDNEGLF-VGAAGLLGLGKGKLSFPGQTGHRF-NQKFSYCL 299

Query: 216 MPYNTDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSG 275
           +  +   + SS   +   + V      T  L      T Y + L GISVG T VP   + 
Sbjct: 300 VDRSASSKPSS--VVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTAS 359

Query: 276 -----PPAKGNAVLDTGTPPTLLPKELY------GRLAAEVRRHIPSKPVDDDTLCYKDN 335
                    G  ++D+GT  T L +  Y       R+ A+  +  P   +  DT     N
Sbjct: 360 LFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSL-FDTCFDLSN 419

Query: 336 LGDL---VMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVG 395
           + ++    + LHF G      +T       ++G FCF   G     ++IGN     F V 
Sbjct: 420 MNEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVV 479

[Close]

BLAST of Csor.00g066680.m01 vs. NCBI nr
Match: KAG6601729.1 (Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 804 bits (2076), Expect = 1.12e-293
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0


Query: 1   MQSHGAYHIYRSRTTFHRRVHRRQRRWSQAGTHPTPTLTRQRFTDGSQITNLAGNQRIYS 60
           MQSHGAYHIYRSRTTFHRRVHRRQRRWSQAGTHPTPTLTRQRFTDGSQITNLAGNQRIYS
Sbjct: 1   MQSHGAYHIYRSRTTFHRRVHRRQRRWSQAGTHPTPTLTRQRFTDGSQITNLAGNQRIYS 60

Query: 61  ENRRRNAADGGACNPRHWQRFILGSVSSMCKMLPANESIYDPSKSSTFRTLSCKSPQCHL 120
           ENRRRNAADGGACNPRHWQRFILGSVSSMCKMLPANESIYDPSKSSTFRTLSCKSPQCHL
Sbjct: 61  ENRRRNAADGGACNPRHWQRFILGSVSSMCKMLPANESIYDPSKSSTFRTLSCKSPQCHL 120

Query: 121 RGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSRSGATTPFSGVVFGCGHNNSGTF 180
           RGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSRSGATTPFSGVVFGCGHNNSGTF
Sbjct: 121 RGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSRSGATTPFSGVVFGCGHNNSGTF 180

Query: 181 NANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYNTDPRISSSLSIGSGSEVKGPGV 240
           NANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYNTDPRISSSLSIGSGSEVKGPGV
Sbjct: 181 NANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYNTDPRISSSLSIGSGSEVKGPGV 240

Query: 241 ITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAKGNAVLDTGTPPTLLPKELYGRL 300
           ITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAKGNAVLDTGTPPTLLPKELYGRL
Sbjct: 241 ITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAKGNAVLDTGTPPTLLPKELYGRL 300

Query: 301 AAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAM 360
           AAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAM
Sbjct: 301 AAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAM 360

Query: 361 GVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKIG 399
           GVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKIG
Sbjct: 361 GVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKIG 399

[Close]

BLAST of Csor.00g066680.m01 vs. NCBI nr
Match: KAG6601733.1 (Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 603 bits (1554), Expect = 3.33e-214
Identity = 301/301 (100.00%), Postives = 301/301 (100.00%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 158
           IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR
Sbjct: 96  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 155

Query: 159 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 218
           SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN
Sbjct: 156 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 215

Query: 219 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 278
           TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK
Sbjct: 216 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 275

Query: 279 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 338
           GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD
Sbjct: 276 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 335

Query: 339 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 398
           LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI
Sbjct: 336 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 395

[Close]

BLAST of Csor.00g066680.m01 vs. NCBI nr
Match: KAG6601726.1 (Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 603 bits (1554), Expect = 3.33e-214
Identity = 301/301 (100.00%), Postives = 301/301 (100.00%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 158
           IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR
Sbjct: 96  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 155

Query: 159 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 218
           SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN
Sbjct: 156 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 215

Query: 219 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 278
           TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK
Sbjct: 216 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 275

Query: 279 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 338
           GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD
Sbjct: 276 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 335

Query: 339 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 398
           LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI
Sbjct: 336 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 395

[Close]

BLAST of Csor.00g066680.m01 vs. NCBI nr
Match: XP_023525703.1 (aspartic proteinase CDR1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 593 bits (1528), Expect = 2.61e-210
Identity = 295/301 (98.01%), Postives = 298/301 (99.00%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 158
           IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR
Sbjct: 92  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 151

Query: 159 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 218
           SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN
Sbjct: 152 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 211

Query: 219 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 278
           TDPRISSSLSIGSGSEVKGPGVITAQLVRT DQTSYSLTLTGISVGKTLVPYS SGPPAK
Sbjct: 212 TDPRISSSLSIGSGSEVKGPGVITAQLVRTADQTSYSLTLTGISVGKTLVPYSTSGPPAK 271

Query: 279 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 338
           GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKP+DDDTLCYKDNLGDLVMTLHF+GGVD
Sbjct: 272 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPIDDDTLCYKDNLGDLVMTLHFDGGVD 331

Query: 339 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 398
           LRLSTVQTFNKM DGSFCFTAMGVDDKDA+IGNSMMANFLVGYDIDNMTVSFKPTDCTKI
Sbjct: 332 LRLSTVQTFNKMPDGSFCFTAMGVDDKDAVIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 391

[Close]

BLAST of Csor.00g066680.m01 vs. NCBI nr
Match: XP_022929935.1 (aspartic proteinase CDR1-like [Cucurbita moschata])

HSP 1 Score: 588 bits (1516), Expect = 2.02e-208
Identity = 293/301 (97.34%), Postives = 296/301 (98.34%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 158
           IYDPSKS TFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR
Sbjct: 96  IYDPSKSLTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 155

Query: 159 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 218
           SGA T FSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN
Sbjct: 156 SGAKTSFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 215

Query: 219 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 278
           TDPRISSSLSIGSGSEVKGPGVIT QLVRTPDQTSYSLTLTGISVGKTLVPYS SGPPAK
Sbjct: 216 TDPRISSSLSIGSGSEVKGPGVITTQLVRTPDQTSYSLTLTGISVGKTLVPYSTSGPPAK 275

Query: 279 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 338
           GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKP+DDDTLCYKDNLGDLVMTLHF+GGVD
Sbjct: 276 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPIDDDTLCYKDNLGDLVMTLHFDGGVD 335

Query: 339 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 398
           LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNS+MANFLVGYDIDNMTVSFKPTDCTKI
Sbjct: 336 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSIMANFLVGYDIDNMTVSFKPTDCTKI 395

[Close]

BLAST of Csor.00g066680.m01 vs. ExPASy TrEMBL
Match: A0A6J1EVM9 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111436390 PE=3 SV=1)

HSP 1 Score: 588 bits (1516), Expect = 9.79e-209
Identity = 293/301 (97.34%), Postives = 296/301 (98.34%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 158
           IYDPSKS TFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR
Sbjct: 96  IYDPSKSLTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 155

Query: 159 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 218
           SGA T FSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN
Sbjct: 156 SGAKTSFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 215

Query: 219 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 278
           TDPRISSSLSIGSGSEVKGPGVIT QLVRTPDQTSYSLTLTGISVGKTLVPYS SGPPAK
Sbjct: 216 TDPRISSSLSIGSGSEVKGPGVITTQLVRTPDQTSYSLTLTGISVGKTLVPYSTSGPPAK 275

Query: 279 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 338
           GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKP+DDDTLCYKDNLGDLVMTLHF+GGVD
Sbjct: 276 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPIDDDTLCYKDNLGDLVMTLHFDGGVD 335

Query: 339 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 398
           LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNS+MANFLVGYDIDNMTVSFKPTDCTKI
Sbjct: 336 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSIMANFLVGYDIDNMTVSFKPTDCTKI 395

[Close]

BLAST of Csor.00g066680.m01 vs. ExPASy TrEMBL
Match: A0A6J1EQ75 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111436389 PE=3 SV=1)

HSP 1 Score: 587 bits (1513), Expect = 2.80e-208
Identity = 293/301 (97.34%), Postives = 298/301 (99.00%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 158
           IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGT+TCKYGYGYGSGSTQGELATEKMAVTSR
Sbjct: 96  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTNTCKYGYGYGSGSTQGELATEKMAVTSR 155

Query: 159 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 218
           SGATTPFSGVVFGCGHNNSGTFNANEMGLIG GRGAISFVSQIGPSVGG+KFSLCLMPYN
Sbjct: 156 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGLGRGAISFVSQIGPSVGGKKFSLCLMPYN 215

Query: 219 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 278
           TDPRISSSLSIGSGSEVKG GVITAQLVRTPDQTSYSLTLTGISVGKTLVPYS SGPPAK
Sbjct: 216 TDPRISSSLSIGSGSEVKGLGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSTSGPPAK 275

Query: 279 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 338
           GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKD+LGDLVMTLHF+GGVD
Sbjct: 276 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDHLGDLVMTLHFDGGVD 335

Query: 339 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 398
           LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGN+MMANFLVGYDIDNMTVSFKPTDCTKI
Sbjct: 336 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNNMMANFLVGYDIDNMTVSFKPTDCTKI 395

[Close]

BLAST of Csor.00g066680.m01 vs. ExPASy TrEMBL
Match: A0A6J1JIJ5 (aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111484909 PE=3 SV=1)

HSP 1 Score: 583 bits (1503), Expect = 9.31e-207
Identity = 291/301 (96.68%), Postives = 294/301 (97.67%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 158
           IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKY YGYGSGSTQGELA+EKMAVTSR
Sbjct: 96  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYSYGYGSGSTQGELASEKMAVTSR 155

Query: 159 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 218
           SGATTPF GVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN
Sbjct: 156 SGATTPFPGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 215

Query: 219 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 278
           TDPRISSSLSIGSGSEVKGPGVITAQLVRT DQTSYSLTLTGISV KTLVPYS SGPPAK
Sbjct: 216 TDPRISSSLSIGSGSEVKGPGVITAQLVRTSDQTSYSLTLTGISVRKTLVPYSTSGPPAK 275

Query: 279 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 338
           GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKP+DDDTLCYKDNLGDLVMTLHF+GGVD
Sbjct: 276 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPIDDDTLCYKDNLGDLVMTLHFDGGVD 335

Query: 339 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 398
           LRLSTVQTFNKM DGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTK 
Sbjct: 336 LRLSTVQTFNKMPDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKA 395

[Close]

BLAST of Csor.00g066680.m01 vs. ExPASy TrEMBL
Match: A0A6J1J4I9 (aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111482577 PE=3 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 1.26e-204
Identity = 289/301 (96.01%), Postives = 292/301 (97.01%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 158
           IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKY YGYGSGSTQGELA+EKMAVTSR
Sbjct: 96  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYNYGYGSGSTQGELASEKMAVTSR 155

Query: 159 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 218
           SGATTPF GVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN
Sbjct: 156 SGATTPFPGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 215

Query: 219 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 278
           TDPRISSSLSIGSGSEVKGPGVITAQLVRT DQTSYSLTLTGISV KTLVPYS S PPAK
Sbjct: 216 TDPRISSSLSIGSGSEVKGPGVITAQLVRTSDQTSYSLTLTGISVRKTLVPYSTSRPPAK 275

Query: 279 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 338
           GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKP+DDDTLCYKDNLGDLVMTLHF+GGVD
Sbjct: 276 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPIDDDTLCYKDNLGDLVMTLHFDGGVD 335

Query: 339 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 398
           LRLSTVQTFNKM DGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCT  
Sbjct: 336 LRLSTVQTFNKMPDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTTA 395

[Close]

BLAST of Csor.00g066680.m01 vs. ExPASy TrEMBL
Match: A0A6J1ID07 (aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111473899 PE=3 SV=1)

HSP 1 Score: 575 bits (1483), Expect = 1.03e-203
Identity = 288/301 (95.68%), Postives = 291/301 (96.68%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGSTQGELATEKMAVTSR 158
           IYDPSKSSTFRTLSCK PQCHLRGSGAACSGTDTCKY YGYGSGSTQGELA+EKMAVTSR
Sbjct: 96  IYDPSKSSTFRTLSCKLPQCHLRGSGAACSGTDTCKYSYGYGSGSTQGELASEKMAVTSR 155

Query: 159 SGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 218
           SGATTPF GVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN
Sbjct: 156 SGATTPFPGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPYN 215

Query: 219 TDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPYSMSGPPAK 278
           TDPRISSSLSIGSGSEVKGPGVITAQLVRT DQTSYSLTLTGISV KTLVPYS SGPPAK
Sbjct: 216 TDPRISSSLSIGSGSEVKGPGVITAQLVRTSDQTSYSLTLTGISVRKTLVPYSTSGPPAK 275

Query: 279 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDDTLCYKDNLGDLVMTLHFEGGVD 338
           GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKP+DDDTLCYKDNLGDLVMTLHF+ GVD
Sbjct: 276 GNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPIDDDTLCYKDNLGDLVMTLHFDDGVD 335

Query: 339 LRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKI 398
           LRLSTVQTFNKM DGSFCFTAMGVD KDALIGNSMMANFLVGYDIDNMTVSFKPTDCTK 
Sbjct: 336 LRLSTVQTFNKMPDGSFCFTAMGVDHKDALIGNSMMANFLVGYDIDNMTVSFKPTDCTKA 395

[Close]

BLAST of Csor.00g066680.m01 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 202.2 bits (513), Expect = 7.7e-52
Identity = 120/313 (38.34%), Postives = 176/313 (56.23%), Query Frame = 0


Query: 97  ESIYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTD-TCKYGYGYGSGS-TQGELATEKMA 156
           + ++DP  SST++ +SC S QC    + A+CS  D TC Y   YG  S T+G +A + + 
Sbjct: 129 DPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLT 188

Query: 157 VTSRSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCL 216
           + S          ++ GCGHNN+GTFN    G++G G G +S + Q+G S+ G KFS CL
Sbjct: 189 LGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDG-KFSYCL 248

Query: 217 MPYNTDPRISSSLSIGSGSEVKGPGVITAQLV-RTPDQTSYSLTLTGISVGKTLVPYSMS 276
           +P  +    +S ++ G+ + V G GV++  L+ +   +T Y LTL  ISVG   + YS S
Sbjct: 249 VPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSGS 308

Query: 277 -GPPAKGNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDD----TLCYKDNLGDL- 336
               ++GN ++D+GT  TLLP E Y  L   V   I ++   D     +LCY    GDL 
Sbjct: 309 DSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSAT-GDLK 368

Query: 337 --VMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDN 396
             V+T+HF+ G D++L +   F ++S+   CF   G     ++ GN    NFLVGYD  +
Sbjct: 369 VPVITMHFD-GADVKLDSSNAFVQVSEDLVCFAFRG-SPSFSIYGNVAQMNFLVGYDTVS 428

Query: 397 MTVSFKPTDCTKI 399
            TVSFKPTDC K+
Sbjct: 429 KTVSFKPTDCAKM 437

[Close]

BLAST of Csor.00g066680.m01 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 193.0 bits (489), Expect = 4.7e-49
Identity = 113/308 (36.69%), Postives = 175/308 (56.82%), Query Frame = 0


Query: 99  IYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGS-TQGELATEKMAVTS 158
           ++DP +SST+R +SC S QC      +  +  +TC Y   YG  S T+G++A + + + S
Sbjct: 127 LFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAVDTVTMGS 186

Query: 159 RSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCLMPY 218
                     ++ GCGH N+GTF+    G+IG G G+ S VSQ+  S+ G KFS CL+P+
Sbjct: 187 SGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSING-KFSYCLVPF 246

Query: 219 NTDPRISSSLSIGSGSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLVPY-SMSGPP 278
            ++  ++S ++ G+   V G GV++  +V+    T Y L L  ISVG   + + S     
Sbjct: 247 TSETGLTSKINFGTNGIVSGDGVVSTSMVKKDPATYYFLNLEAISVGSKKIQFTSTIFGT 306

Query: 279 AKGNAVLDTGTPPTLLPKELYGRLAAEVRRHIPSKPVDDD----TLCYKDNLGDLV--MT 338
            +GN V+D+GT  TLLP   Y  L + V   I ++ V D     +LCY+D+    V  +T
Sbjct: 307 GEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILSLCYRDSSSFKVPDIT 366

Query: 339 LHFEGGVDLRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSMMANFLVGYDIDNMTVSF 398
           +HF+GG D++L  + TF  +S+   CF A   +++  + GN    NFLVGYD  + TVSF
Sbjct: 367 VHFKGG-DVKLGNLNTFVAVSEDVSCF-AFAANEQLTIFGNLAQMNFLVGYDTVSGTVSF 426

[Close]

BLAST of Csor.00g066680.m01 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 185.7 bits (470), Expect = 7.5e-47
Identity = 118/323 (36.53%), Postives = 178/323 (55.11%), Query Frame = 0


Query: 96  NESIYDPSKSSTFRTLSCKSPQCH-LRGSGAAC-SGTDTCKYGYGYGSGS-TQGELATEK 155
           N  ++D  KSST++T SC S  C  L      C    D CKY Y YG  S T+G++ATE 
Sbjct: 123 NSPLFDKKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTKGDVATET 182

Query: 156 MAVTSRSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSL 215
           +++ S SG++  F G VFGCG+NN GTF     G+IG G G +S VSQ+G S+ G+KFS 
Sbjct: 183 ISIDSSSGSSVSFPGTVFGCGYNNGGTFEETGSGIIGLGGGPLSLVSQLGSSI-GKKFSY 242

Query: 216 CLMPYNTDPRISSSLSIGSGSEVKGP----GVITAQLVRTPDQTSYSLTLTGISVGKTLV 275
           CL         +S +++G+ S    P      +T  L++   +T Y LTL  ++VGKT +
Sbjct: 243 CLSHTAATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTLEAVTVGKTKL 302

Query: 276 PYS-----MSGPPAK--GNAVLDTGTPPTLLPKELYGRLAAEVRRHIP-SKPVDDD---- 335
           PY+     ++G  +K  GN ++D+GT  TLL    Y      V   +  +K V D     
Sbjct: 303 PYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKRVSDPQGLL 362

Query: 336 TLCYKD---NLGDLVMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGNSM 395
           T C+K     +G   +T+HF    D++LS +  F K+++ + C + +   +  A+ GN +
Sbjct: 363 THCFKSGDKEIGLPAITMHFT-NADVKLSPINAFVKLNEDTVCLSMIPTTEV-AIYGNMV 422

Query: 396 MANFLVGYDIDNMTVSFKPTDCT 397
             +FLVGYD++  TVSF+  DC+
Sbjct: 423 QMDFLVGYDLETKTVSFQRMDCS 442

[Close]

BLAST of Csor.00g066680.m01 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 175.3 bits (443), Expect = 1.0e-43
Identity = 115/325 (35.38%), Postives = 175/325 (53.85%), Query Frame = 0


Query: 96  NESIYDPSKSSTFRTLSCKSPQCH-LRGSGAAC-SGTDTCKYGYGYGSGS-TQGELATEK 155
           N  I+D  KSST+++  C S  C  L  +   C    + CKY Y YG  S ++G++ATE 
Sbjct: 123 NGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATET 182

Query: 156 MAVTSRSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSL 215
           +++ S SG+   F G VFGCG+NN GTF+    G+IG G G +S +SQ+G S+  +KFS 
Sbjct: 183 VSIDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLGSSI-SKKFSY 242

Query: 216 CLMPYNTDPRISSSLSIGS----GSEVKGPGVITAQLVRTPDQTSYSLTLTGISVGKTLV 275
           CL   +     +S +++G+     S  K  GV++  LV     T Y LTL  ISVGK  +
Sbjct: 243 CLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKKI 302

Query: 276 PYSMSG---------PPAKGNAVLDTGTPPTLLPKELYGRLAAEVRRHIP-SKPVDDD-- 335
           PY+ S              GN ++D+GT  TLL    + + ++ V   +  +K V D   
Sbjct: 303 PYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQG 362

Query: 336 --TLCYKDNLGDL---VMTLHFEGGVDLRLSTVQTFNKMSDGSFCFTAMGVDDKDALIGN 395
             + C+K    ++    +T+HF  G D+RLS +  F K+S+   C + +   +  A+ GN
Sbjct: 363 LLSHCFKSGSAEIGLPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMVPTTEV-AIYGN 422

Query: 396 SMMANFLVGYDIDNMTVSFKPTDCT 397
               +FLVGYD++  TVSF+  DC+
Sbjct: 423 FAQMDFLVGYDLETRTVSFQHMDCS 444

[Close]

BLAST of Csor.00g066680.m01 vs. TAIR 10
Match: AT2G28010.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 137.5 bits (345), Expect = 2.3e-32
Identity = 102/315 (32.38%), Postives = 150/315 (47.62%), Query Frame = 0


Query: 96  NESIYDPSKSSTFRTLSCKSPQCHLRGSGAACSGTDTCKYGYGYGSGS-TQGELATEKMA 155
           N  I+DPSKSSTF+   C                  +C Y   Y   + T G LATE + 
Sbjct: 103 NAPIFDPSKSSTFKEKRCDG---------------HSCPYEVDYFDHTYTMGTLATETIT 162

Query: 156 VTSRSGATTPFSGVVFGCGHNNSGTFNANEMGLIGFGRGAISFVSQIGPSVGGRKFSLCL 215
           + S SG        + GCGHNNS  F  +  G++G   G  S ++Q+G    G      L
Sbjct: 163 LHSTSGEPFVMPETIIGCGHNNS-WFKPSFSGMVGLNWGPSSLITQMGGEYPG------L 222

Query: 216 MPYNTDPRISSSLSIGSGSEVKGPGVI-TAQLVRTPDQTSYSLTLTGISVGKTLV-PYSM 275
           M Y    + +S ++ G+ + V G GV+ T   + T     Y L L  +SVG T +     
Sbjct: 223 MSYCFSGQGTSKINFGANAIVAGDGVVSTTMFMTTAKPGFYYLNLDAVSVGNTRIETMGT 282

Query: 276 SGPPAKGNAVLDTGTPPTLLPKELYGRLAAEVRRHI-----PSKPVDDDTLCYKDNLGDL 335
           +    +GN V+D+GT  T  P   Y  L  +   H+      + P  +D LCY  +  D+
Sbjct: 283 TFHALEGNIVIDSGTTLTYFPVS-YCNLVRQAVEHVVTAVRAADPTGNDMLCYNSDTIDI 342

Query: 336 --VMTLHFEGGVDLRLSTVQTFNKMSDGS-FCFTAM-GVDDKDALIGNSMMANFLVGYDI 395
             V+T+HF GGVDL L     + + ++G  FC   +     ++A+ GN    NFLVGYD 
Sbjct: 343 FPVITMHFSGGVDLVLDKYNMYMESNNGGVFCLAIICNSPTQEAIFGNRAQNNFLVGYDS 394

Query: 396 DNMTVSFKPTDCTKI 399
            ++ VSF PT+C+ +
Sbjct: 403 SSLLVSFSPTNCSAL 394

The following BLAST results are available for this feature:

BLAST of Csor.00g066680.m01 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6XBF8	1.1e-50	38.34	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q3EBM5	1.4e-42	35.38	Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561...	[more]
Q766C3	1.1e-29	32.59	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	4.2e-26	33.12	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	7.9e-25	30.48	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]

BLAST of Csor.00g066680.m01 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6601729.1	1.12e-293	100.00	Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6601733.1	3.33e-214	100.00	Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6601726.1	3.33e-214	100.00	Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023525703.1	2.61e-210	98.01	aspartic proteinase CDR1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022929935.1	2.02e-208	97.34	aspartic proteinase CDR1-like [Cucurbita moschata]	[more]

BLAST of Csor.00g066680.m01 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EVM9	9.79e-209	97.34	aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111436390 PE=3...	[more]
A0A6J1EQ75	2.80e-208	97.34	aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111436389 PE=3...	[more]
A0A6J1JIJ5	9.31e-207	96.68	aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111484909 PE=3 S...	[more]
A0A6J1J4I9	1.26e-204	96.01	aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111482577 PE=3 S...	[more]
A0A6J1ID07	1.03e-203	95.68	aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111473899 PE=3 S...	[more]

BLAST of Csor.00g066680.m01 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G33340.1	7.7e-52	38.34	Eukaryotic aspartyl protease family protein	[more]
AT1G64830.1	4.7e-49	36.69	Eukaryotic aspartyl protease family protein	[more]
AT1G31450.1	7.5e-47	36.53	Eukaryotic aspartyl protease family protein	[more]
AT2G35615.1	1.0e-43	35.38	Eukaryotic aspartyl protease family protein	[more]
AT2G28010.1	2.3e-32	32.38	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 237..398 e-value: 4.0E-33 score: 116.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 72..230 e-value: 2.9E-26 score: 94.5
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 94..395
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 90..231 e-value: 1.0E-32 score: 113.8
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 254..391 e-value: 4.4E-18 score: 65.6
None	No IPR available	PANTHER	PTHR47967:SF39	ASPARTYL PROTEASE FAMILY PROTEIN, PUTATIVE-RELATED	coord: 96..397
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 96..397
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 66..391 score: 22.148329
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 133..395 e-value: 1.71473E-58 score: 189.781

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csor.00g066680	Csor.00g066680	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g066680.m01.CDS02	Csor.00g066680.m01.CDS02	CDS
Csor.00g066680.m01.CDS01	Csor.00g066680.m01.CDS01	CDS

The following initial feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g066680.m01.initial01	Csor.00g066680.m01.initial01	initial

The following start_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g066680.m01.start_codon01	Csor.00g066680.m01.start_codon01	start_codon

The following intron feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g066680.m01.intron01	Csor.00g066680.m01.intron01	intron

The following terminal feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g066680.m01.terminal01	Csor.00g066680.m01.terminal01	terminal

The following stop_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csor.00g066680.m01.stop_codon01	Csor.00g066680.m01.stop_codon01	stop_codon

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csor.00g066680.m01	Csor.00g066680.m01-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity