Csor.00g021200.m01 (mRNA) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g021200.m01 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.4e-28
Identity = 86/253 (33.99%), Postives = 122/253 (48.22%), Query Frame = 0

Query: 38  GRDGLCLQTAPLSIY-YTPTKLTICDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKP 97
           GRDG C +         TP +L+ C E  S+Q W    DGTI++     CL   S     
Sbjct: 323 GRDGFCAEVKNGDEKDGTPVQLSSCGE-QSNQQWTFSTDGTIQSLG--KCLTTSS----- 382

Query: 98  IGTNVVLVDCTKEIRSDMKWTKTNDGTIHHDESGLVLT---GKQGQYVSLEVDEGAPSQG 157
              +V++ +C        KW  + DGTI +  SGLVLT     +G  VSLE +  A  QG
Sbjct: 383 ---SVMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAARQG 442

Query: 158 WEATERPYPKAANIKWHNNLCLQFGDGEFSLGLSACSSKYDKR--QRWVLYGDGTIRGNG 217
           W       P    I  +  +CL+   G   + L  CS K   +  Q+W LYGDGTIR N 
Sbjct: 443 W-IVGNVEPLVTFIVGYEQMCLETNPGNNDVSLGDCSVKSASKVDQKWALYGDGTIRVNN 502

Query: 218 DSNVCLTSLLSSS---VVVSKCADMPQQRWALAADNTIGHPNTNLVMDARTFVRLSPMVL 277
           D ++C+TS   SS   +++ KC     QRW    D TI +P++ LVM           ++
Sbjct: 503 DRSLCVTSEGKSSNEPIIILKCLGWANQRWVFNTDGTISNPDSKLVMHVDQNDVPLRKII 562

Query: 278 VAKRAGTDSQRWI 282
           ++  +GT +Q+WI
Sbjct: 563 LSHPSGTSNQQWI 563

BLAST of Csor.00g021200.m01 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 120.9 bits (302), Expect = 2.3e-26
Identity = 76/254 (29.92%), Postives = 125/254 (49.21%), Query Frame = 0

Query: 35  HLLGRDGLCLQTA-PLSIYYTPTKLTICDESDSDQLWAIVDDGTIRADNDRSCLVPHSRG 94
           +++GRDGLC+          TP +L  C  +  +Q W    D TIR+     C+   + G
Sbjct: 311 NIVGRDGLCVDVRNGYDTDGTPLQLWPCG-TQRNQRWTFDSDDTIRSMG--KCMT--ANG 370

Query: 95  IKPIGTNVVLVDCTKEIRSDMKWTKTNDGTIHHDESGLVLTGKQG---QYVSLEVDEGAP 154
           +   G+N+V+ +C+    + +KW    DG+I +  SGLV+T  +      + LE +  A 
Sbjct: 371 LNN-GSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLEDNIYAA 430

Query: 155 SQGWEATERPYPKAANIKWHNNLCLQFGDGEFSLGLSACSSKYDKRQRWVLYGDGTIRGN 214
           SQGW  T    P  A+I  +  +CLQ       + +  C +    +Q+W LYGD TIR N
Sbjct: 431 SQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEAT-SLQQQWALYGDRTIRVN 490

Query: 215 GDSNVCLTSLLSSS---VVVSKCADMPQQRWALAADNTIGHPNTNLVMDARTFVRLSPMV 274
               +C+T+   +S   +++ KC  +P QRW   +D  I +P +  VMD R        +
Sbjct: 491 STRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMDVRASNVSLREI 550

Query: 275 LVAKRAGTDSQRWI 282
           ++    G  +Q+W+
Sbjct: 551 IIFPATGNPNQQWV 557

BLAST of Csor.00g021200.m01 vs. ExPASy Swiss-Prot
Match: P82683 (Beta-galactoside-specific lectin 3 OS=Viscum album OX=3972 PE=1 SV=2)

HSP 1 Score: 112.8 bits (281), Expect = 6.2e-24
Identity = 76/255 (29.80%), Postives = 118/255 (46.27%), Query Frame = 0

Query: 36  LLGRDGLCLQTAPLSIYY-TPTKLTIC-DESDSDQLWAIVDDGTIRADNDRSCLVPHSRG 95
           ++GRDGLC+       +   P +L+ C   +D +QLW I  DGTIR++    CL  +   
Sbjct: 320 IVGRDGLCVDVRDGKFHNGNPIQLSPCKSNTDPNQLWTIRRDGTIRSNG--RCLTTYG-- 379

Query: 96  IKPIGTNVVLVDCTKEIRSDMKWTKTNDGTIHHDESGLVL---TGKQGQYVSLEVDEGAP 155
               G  V++ DC   +R    W    +GTI +  S LVL   +G  G  ++++    + 
Sbjct: 380 -YTAGVYVMIFDCNTAVREATLWQIWGNGTIINPRSNLVLGAASGSSGTTLTVQTQVYSL 439

Query: 156 SQGWEATERPYPKAANIKWHNNLCLQFGDGEFSLGLSACSSKYDKRQRWVLYGDGTIRGN 215
            QGW A     P+   I    +LC++      S+ +  C S   + Q W LYGDG+IR  
Sbjct: 440 GQGWLAGNDTAPREVTIYGFRDLCMEANGA--SVWVETCGSS-TENQNWALYGDGSIRPK 499

Query: 216 GDSNVCLT----SLLSSSVVVSKCADMPQQRWALAADNTIGHPNTNLVMDARTFVRLSPM 275
            + + CLT    S+ +   +VS  A    QRW    + TI + N  LVMD          
Sbjct: 500 QNQDQCLTCQGDSVATVINIVSCSAGSSGQRWVFTNEGTILNLNNGLVMDVAQSNPSLRR 559

Query: 276 VLVAKRAGTDSQRWI 282
           +++    G  +Q W+
Sbjct: 560 IIIYPATGNPNQMWL 566

BLAST of Csor.00g021200.m01 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.1e-23
Identity = 86/252 (34.13%), Postives = 119/252 (47.22%), Query Frame = 0

Query: 38  GRDGLCLQTA-PLSIYYTPTKLTICDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKP 97
           G DGLC+       I   P +L  C  ++ +QLW    DGTIR      CL   S     
Sbjct: 327 GWDGLCVDVRYGHYIDGNPVQLRPCG-NECNQLWTFRTDGTIRWLG--KCLTASS----- 386

Query: 98  IGTNVVLVDCTKEIRSDMKWTKTNDGTIHHDESGLVLTGKQ---GQYVSLEVDEGAPSQG 157
              +V++ DC        KW  + DGTI +  SGLVLT  Q   G  +SLE +  A  QG
Sbjct: 387 ---SVMIYDCNTVPPEATKWVVSIDGTITNPHSGLVLTAPQAAEGTALSLENNIHAARQG 446

Query: 158 WEATERPYPKAANIKWHNNLCLQFGDGEFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDS 217
           W   +   P    I  +  +CL+       + L  C      +Q W LYGDGTIR N + 
Sbjct: 447 WTVGD-VEPLVTFIVGYKQMCLRENGENNFVWLEDCVLN-RVQQEWALYGDGTIRVNSNR 506

Query: 218 NVCLTS---LLSSSVVVSKCADMPQQRWALAADNTIGHPNTNLVMD-ARTFVRLSPMVLV 277
           ++C+TS     S  +V+ KC     QRW    + TI +PN  L+MD A+  V L  ++L 
Sbjct: 507 SLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNPNAKLLMDVAQRDVSLRKIILY 564

Query: 278 AKRAGTDSQRWI 282
            +  G  +Q+WI
Sbjct: 567 -RPTGNPNQQWI 564

BLAST of Csor.00g021200.m01 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.1e-23
Identity = 88/253 (34.78%), Postives = 120/253 (47.43%), Query Frame = 0

Query: 38  GRDGLCLQTAP-LSIYYTPTKLTICDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKP 97
           G DGLC+       I     +L  C  ++ +QLW    DGTIR      CL   S     
Sbjct: 327 GWDGLCVDVRDGHYIDGNTVQLGPCG-NECNQLWTFRTDGTIRWLG--KCLTTSS----- 386

Query: 98  IGTNVVLVDCTKEIRSDMKWTKTNDGTIHHDESGLVLTGKQ---GQYVSLEVDEGAPSQG 157
              +V++ DC        KW  + DGTI +  SGLVLT  Q   G  +SLE +  A  QG
Sbjct: 387 ---SVMIYDCNTVPPEATKWVVSTDGTITNPRSGLVLTAPQAAEGTALSLENNIHAARQG 446

Query: 158 WEATERPYPKAANIKWHNNLCL-QFGDGEFSLGLSACSSKYDKRQRWVLYGDGTIRGNGD 217
           W   +   P    I  +  +CL + G+  F + L  C       Q W LYGDGTIR N +
Sbjct: 447 WTVGD-VEPLVTFIVGYKQMCLTENGENNF-VWLEDCVLN-RVEQEWALYGDGTIRVNSN 506

Query: 218 SNVCLTS---LLSSSVVVSKCADMPQQRWALAADNTIGHPNTNLVMD-ARTFVRLSPMVL 277
            ++C+TS     S  +V+ KC     QRW    + TI +PN  LVMD A++ V L  ++L
Sbjct: 507 RSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNPNAKLVMDVAQSNVSLRKIIL 564

Query: 278 VAKRAGTDSQRWI 282
                G  +Q+WI
Sbjct: 567 YPP-TGNPNQQWI 564

BLAST of Csor.00g021200.m01 vs. NCBI nr
Match: KAG6600357.1 (hypothetical protein SDJN03_05590, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 589 bits (1519), Expect = 1.07e-212
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0

Query: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60
           MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI
Sbjct: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60

Query: 61  CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN
Sbjct: 61  CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG
Sbjct: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180

Query: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240
           EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL
Sbjct: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240

Query: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWIVY 283
           AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWIVY
Sbjct: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWIVY 283

BLAST of Csor.00g021200.m01 vs. NCBI nr
Match: KAG7031016.1 (hypothetical protein SDJN02_05055, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 581 bits (1498), Expect = 1.05e-208
Identity = 279/281 (99.29%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60
           MEKTRQVVLCMVVAFSLATHI TALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI
Sbjct: 1   MEKTRQVVLCMVVAFSLATHISTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60

Query: 61  CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           CDES+SDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN
Sbjct: 61  CDESESDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG
Sbjct: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180

Query: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240
           EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL
Sbjct: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240

Query: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWI 281
           AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWI
Sbjct: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWI 281

BLAST of Csor.00g021200.m01 vs. NCBI nr
Match: XP_023513493.1 (ribosome-inactivating protein SNAI'-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 569 bits (1467), Expect = 8.75e-205
Identity = 274/283 (96.82%), Postives = 280/283 (98.94%), Query Frame = 0

Query: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60
           MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHL+GRDGLCLQTAPLSIYYTPTKLT 
Sbjct: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60

Query: 61  CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           CDESDSDQLWAIVDDGTIRADNDR+CLVPHSRGIKPIGTNVVL+DCTKEIRSDMKWTKTN
Sbjct: 61  CDESDSDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLIDCTKEIRSDMKWTKTN 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTI HD+SGLVLTGKQGQYV+LEVDEGAPSQGWEATERPYPKAANIKWH+NLCLQFGDG
Sbjct: 121 DGTIRHDKSGLVLTGKQGQYVTLEVDEGAPSQGWEATERPYPKAANIKWHDNLCLQFGDG 180

Query: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240
           EFSLGLSACSSKYDKRQRWVLYGDGTIR NGDSNVCLTSLLSSSVVVSKCADMPQQRWAL
Sbjct: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIR-NGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240

Query: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWIVY 283
           AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWIVY
Sbjct: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWIVY 282

BLAST of Csor.00g021200.m01 vs. NCBI nr
Match: XP_022988756.1 (ribosome-inactivating protein SNAI'-like [Cucurbita maxima])

HSP 1 Score: 561 bits (1447), Expect = 1.02e-201
Identity = 267/283 (94.35%), Postives = 274/283 (96.82%), Query Frame = 0

Query: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60
           MEKTRQV+LCMVVAFSLATHIGT LGYGAFGGRAHL+GRDGLCLQTAPLSIYYTPTKLT 
Sbjct: 1   MEKTRQVLLCMVVAFSLATHIGTTLGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60

Query: 61  CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           CDESDSDQLWAIVDDGTIR DNDR+CLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN
Sbjct: 61  CDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTI HDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPK ANIKWHNNLCLQFGDG
Sbjct: 121 DGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNNLCLQFGDG 180

Query: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240
           EFSLGLSACSSKY+K QRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRW L
Sbjct: 181 EFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWGL 240

Query: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWIVY 283
           AADNTIGHPNTNLVMDARTFVRLSP++LVAKR GT SQ+WIVY
Sbjct: 241 AADNTIGHPNTNLVMDARTFVRLSPLILVAKRTGTYSQQWIVY 283

BLAST of Csor.00g021200.m01 vs. NCBI nr
Match: XP_022941585.1 (nigrin b-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 356 bits (913), Expect = 2.43e-120
Identity = 181/288 (62.85%), Postives = 213/288 (73.96%), Query Frame = 0

Query: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60
           MEKTRQV+LCMVVAFSLATHIG ALGYGAFGGR HL+GRDGLCL T  L  YY PT L+ 
Sbjct: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRTHLVGRDGLCLDTMLLLGYYPPTHLSQ 60

Query: 61  CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           C++  S QLW+I++DGTIR  + R CLVP S+  + +G NVV +DC+KEIR DMKWTK  
Sbjct: 61  CEQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTKNK 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTIHHDESGLVLT K G+ V++EV+E  PSQ WEATE   P  ANIKW +NLCL+  +G
Sbjct: 121 DGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEG 180

Query: 181 EFS-LGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSL---LSSSVVVSKCADMPQQ 240
           E S + L+ CS + DK QRW LYGDGTIR N   ++CLTS      + VVVS+CAD PQQ
Sbjct: 181 ENSYVTLNECS-RDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKPQQ 240

Query: 241 RWALAADNTIGHPNTNLVMDARTFVRL-SPMVLVAKRAGTDSQRWIVY 283
           RW LA D+TI HPNTNLVMD R  V L  PM++V K  G  SQRW +Y
Sbjct: 241 RWGLAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTIY 287

BLAST of Csor.00g021200.m01 vs. ExPASy TrEMBL
Match: A0A6J1JI54 (ribosome-inactivating protein SNAI'-like OS=Cucurbita maxima OX=3661 GN=LOC111485998 PE=4 SV=1)

HSP 1 Score: 561 bits (1447), Expect = 4.92e-202
Identity = 267/283 (94.35%), Postives = 274/283 (96.82%), Query Frame = 0

Query: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60
           MEKTRQV+LCMVVAFSLATHIGT LGYGAFGGRAHL+GRDGLCLQTAPLSIYYTPTKLT 
Sbjct: 1   MEKTRQVLLCMVVAFSLATHIGTTLGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60

Query: 61  CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           CDESDSDQLWAIVDDGTIR DNDR+CLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN
Sbjct: 61  CDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTI HDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPK ANIKWHNNLCLQFGDG
Sbjct: 121 DGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNNLCLQFGDG 180

Query: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240
           EFSLGLSACSSKY+K QRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRW L
Sbjct: 181 EFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWGL 240

Query: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWIVY 283
           AADNTIGHPNTNLVMDARTFVRLSP++LVAKR GT SQ+WIVY
Sbjct: 241 AADNTIGHPNTNLVMDARTFVRLSPLILVAKRTGTYSQQWIVY 283

BLAST of Csor.00g021200.m01 vs. ExPASy TrEMBL
Match: A0A6J1FU34 (nigrin b-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=4 SV=1)

HSP 1 Score: 356 bits (913), Expect = 1.17e-120
Identity = 181/288 (62.85%), Postives = 213/288 (73.96%), Query Frame = 0

Query: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60
           MEKTRQV+LCMVVAFSLATHIG ALGYGAFGGR HL+GRDGLCL T  L  YY PT L+ 
Sbjct: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRTHLVGRDGLCLDTMLLLGYYPPTHLSQ 60

Query: 61  CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           C++  S QLW+I++DGTIR  + R CLVP S+  + +G NVV +DC+KEIR DMKWTK  
Sbjct: 61  CEQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTKNK 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTIHHDESGLVLT K G+ V++EV+E  PSQ WEATE   P  ANIKW +NLCL+  +G
Sbjct: 121 DGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEG 180

Query: 181 EFS-LGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSL---LSSSVVVSKCADMPQQ 240
           E S + L+ CS + DK QRW LYGDGTIR N   ++CLTS      + VVVS+CAD PQQ
Sbjct: 181 ENSYVTLNECS-RDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKPQQ 240

Query: 241 RWALAADNTIGHPNTNLVMDARTFVRL-SPMVLVAKRAGTDSQRWIVY 283
           RW LA D+TI HPNTNLVMD R  V L  PM++V K  G  SQRW +Y
Sbjct: 241 RWGLAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTIY 287

BLAST of Csor.00g021200.m01 vs. ExPASy TrEMBL
Match: A0A6J1IYQ6 (ribosome-inactivating protein SNAI-like OS=Cucurbita maxima OX=3661 GN=LOC111481109 PE=4 SV=1)

HSP 1 Score: 336 bits (861), Expect = 8.73e-113
Identity = 174/288 (60.42%), Postives = 204/288 (70.83%), Query Frame = 0

Query: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60
           MEK RQV+LC+ +AFSLA  I     YGAFGGR HL+GRDGLCL T  LS YY  T L+ 
Sbjct: 1   MEKMRQVLLCIALAFSLA--IAAPSEYGAFGGRTHLVGRDGLCLDTMLLSGYYPQTDLSQ 60

Query: 61  CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           C      QLW I++DGTIRA + R CLVP ++ I P+G NVV++DCTKE+R DMKWTK  
Sbjct: 61  CQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEVRHDMKWTKKK 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTIHHDESGLVLT K G+ V+LE +E APSQ WEATE  YP  ANIKW +NLCL+  +G
Sbjct: 121 DGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLCLKSREG 180

Query: 181 EFS-LGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSL---LSSSVVVSKCADMPQQ 240
           E S + L+ CS + DK QRW LYGDGTIR N   ++CLTS      + VVVS+CAD PQQ
Sbjct: 181 ENSYVTLNECS-RDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSRCADKPQQ 240

Query: 241 RWALAADNTIGHPNTNLVMDA-RTFVRLSPMVLVAKRAGTDSQRWIVY 283
           RW LA DNTI HPNTNLVMD  R    L PM++V K  G  SQRW +Y
Sbjct: 241 RWGLAEDNTINHPNTNLVMDVHREVPLLPPMIVVDKHDGAPSQRWSIY 285

BLAST of Csor.00g021200.m01 vs. ExPASy TrEMBL
Match: A0A6J1FNV0 (nigrin b-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=3 SV=1)

HSP 1 Score: 331 bits (849), Expect = 6.65e-111
Identity = 170/290 (58.62%), Postives = 206/290 (71.03%), Query Frame = 0

Query: 1   MEKTRQVVLCMVVAFSLATH--IGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKL 60
           M+K  QV+ C+ +AFSLATH  I  A GYG+FG R HL+GRDGLCL T  L  YY PT L
Sbjct: 1   MDKMTQVLFCIALAFSLATHTAIAAASGYGSFGSRTHLVGRDGLCLDTMLLLGYYPPTHL 60

Query: 61  TICDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTK 120
           + C++  S QLW+I++DGTIR  + R CLVP S+  + +G NVV +DC+KEIR DMKWTK
Sbjct: 61  SQCEQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTK 120

Query: 121 TNDGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFG 180
             DGTIHHDESGLVLT K G+ V++EV+E  PSQ WEATE   P  ANIKW +NLCL+  
Sbjct: 121 NKDGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSI 180

Query: 181 DGEFS-LGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSL---LSSSVVVSKCADMP 240
           +GE S + L+ CS + DK QRW LYGDGTIR N   ++CLTS      + VVVS+CAD P
Sbjct: 181 EGENSYVTLNECS-RDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKP 240

Query: 241 QQRWALAADNTIGHPNTNLVMDARTFVRL-SPMVLVAKRAGTDSQRWIVY 283
           QQRW LA D+TI HPNTNLVMD R  V L  PM++V K  G  SQRW +Y
Sbjct: 241 QQRWGLAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTIY 289

BLAST of Csor.00g021200.m01 vs. ExPASy TrEMBL
Match: A0A5A7T854 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001540 PE=3 SV=1)

HSP 1 Score: 245 bits (626), Expect = 4.53e-77
Identity = 138/296 (46.62%), Postives = 181/296 (61.15%), Query Frame = 0

Query: 5   RQVVLCMVVAFSLATHIGTALG--------YGAFGGRAHLLGRDGLCLQTAPLSIYYT-- 64
           +++VL ++VAFSL TH+  A+         YG   G  HL+GRDGLCL+ +P   +Y   
Sbjct: 2   KEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPGATHLVGRDGLCLEMSPW--FYRGN 61

Query: 65  --PTKLTICDESDSD-QLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIR 124
             PT+L+ CDE     QLW +V DGTIR  ND+ CLV     +  +    V+ +C KE  
Sbjct: 62  NFPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADV--LVGVINKAVVRECAKESN 121

Query: 125 SDMKWTKTNDGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHN 184
           S+ +WT   DGTI H ESG+VLTG    YV+L+ ++  PSQ WEATE   P  ANI+W N
Sbjct: 122 SNKRWTHKKDGTITHVESGMVLTGDS-DYVTLQSNKNTPSQSWEATESSTPMIANIEWLN 181

Query: 185 NLCLQFGDGEFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTS--LLSSSVVVSK 244
           NLCLQ  D    +GL+ C+ + +K QRW LYGDGTIR N + N CLTS   L   +V+SK
Sbjct: 182 NLCLQSTDDSNHVGLNGCNRE-NKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSK 241

Query: 245 CADMPQQRWALAA-DNTIGHPNTNLVMDAR--TFVRLSPMVLVAKRAGTDSQRWIV 282
           C D PQQRW L A D+TI HPNT++VMD     +   S  V+  +R G+ SQRW +
Sbjct: 242 CEDKPQQRWNLGAKDSTIDHPNTDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291