CsaV3_3G041040.1 (mRNA) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_3G041040.1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	tRNA (Guanosine(18)-2'-O)-methyltransferase
Location	chr3: 33569060 .. 33572151 (+)
Sequence length	1218
RNA-Seq Expression	CsaV3_3G041040.1
Synteny	CsaV3_3G041040.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAGCTAGAAATGATGATTCCGTGCAATGTCAGTGGTATTATGTGAGTTTTTCCAAGTGAGTGAGAACGAAAAGATGAAGAAGTAAGGGAGAGCAACAAAAGAAGAAGTAATATTAAATTTTGGTCAAAACCTATACTTTTAAGAAAAGTTATATCAGTCATCTGCCTGTTGCAAATATGGTAAAATTAGTAATATGAGATGGAATTGTGTTATTTCCTTGTGGTTGGTACCCCTAAAAAAAGATCTTTCTTGTTAGTTCTTAGTAGCTGCTGGGTTGGTTTCTTCTTACCTCTGTCTGAACCGTTGAACCTCTTTCAACGCACATCCATGGCGTCCTGCAGAATCGTATCCCGTTCTTCTCTTTTTCTGCTCCAAAGCTGCAGTCCCCGAAGAACTCAACGGATTTTCTCTTCTGTTTTTCTCCCTTCTCTACACCTTCCCTCCTTCTCTCTGTTCTTCACTAACAGATGTACTGTCTTCTCTCTTTCCCTCTCTTGGAGCCCTATCTTTACATTTCTTTTTATTAGTCTCTTCTTTACTTTATTTCTCTTTTTATTGCTACATAGTCTCAAGAAGCAACCCTAACGCAGTTTCTACAAGATGTTTCGCTTCAATTTCCGCCGCAACATCGCCACTAGAAGTAGAAGATATTGTGGCTGACGTAGAACCACCGGAGGTTCGTACACAAGACACGGTGGAGCACCTGCTCACGCATTCCAATGACGTTGCTAGGTTGATGAAGATGGAACGGAGGTCGGTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGTGGTTTCCGTATCTGGATAAGTATAGCTGTGGAAGTTCTTGGTTGAATAGTGGTGAGATTTTGGAGGCCTTGGATTCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTGGTGCGGAATAGAAGCTACTCCGTTTGTTTGGTGGTGGAGGGATTGACAGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCTGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAACAACCATGGAGGTGAAATTGTTTTCGAATGCATTTGTTTTTTTTTTTTAATTTCTTTCGGGAAAATTGTTTGATTCTGGAATTTGGAAGCTGGTTTGTTTTCAATGTGATGGTGTGGAATTTCATTACTGTTCTTGTGCTTTTACTTTGTTGTTCAATGGACCATTTGAATTCAATCAATTAGTCGAATGCCCTTGCTGGTTAATATGGTTAGAAGGTTAAACCGATCAAATACGTTTGATTTTGATTGGTAATTTTTAAATACGGATGACAATGTGACACAGATTGAGCAATGGAATTCCTGCAAATGTGCATCTCTTGTATATATTGTATCTTTATATGCACATAACCATTGAAAACTTTATACCAAATGTCGATTTTAATGTATTATTTTTCATTGCACAACGTTTATTTTCCATGTGAAAGTATATGTTCATATGTTTTTTCTTCCTTCATTTGCCATCTTTATCTCGCCATTTCATAACATTAAGTCATATTTGTCTTGGGCATGAAACCTTTTCCAGTGCTTGTATTCTCATGCTTACCCTGAATGAGATCATGCTTTGATAAATTAGGGTGCAACATGAGTTCGTTCAGCTTACTAAGAATGGCAAAAATTCCCATTGCTTACATTTTCTTTGTGAATATTTTCTCGTATTTCTTCATCCGACATCATTGCTATTCAATTCAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCTACCCCAGAATGTTTTAAAATTCTGAAATCCCGGGGTTATAGAATAGTCTCTACTCATCTAGGAGCGAATGCGGTATTGATTGATTTTGTAAGCCCTTCTTTCACATTAGAAATTTTGTAAGCTTGTCCTGACAGCTGAATTGCGATTGTCCTGTTGTAGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGTAATTATCTTCTTGCTCCAGTAATATGGAGTGTGATCAAGTTCAAAGCAATTAGTTACGGGATGATTGCAATAGCTGTTTTCTCTATGTTATTCGATGAACATTTTTGAATCACTGCAAGTTGGGAGATAATAGATATCAGTAAAATTTAGGAAATCATAACTGAATACACGCTTAAAAGTAGCAACATTTTTTTGAAATTGACAATTATCTCATGCTTCAGTTAGCAAGTTTCGACGCATGAATGATGTCGGAGTCTGGAGAAACTATACTTGTAGCTATGTTGATTTCTGAGGTTAAGATTTCTTCCTTTTTTAGGGGAATAAGTAATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTTTCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCAATCGGACTTCTCGGCTGGTAACTATAAAAAACCATGTTTGAATACTCTTGGATTCATCAGGCCATAAGGGTTTGGATAGCTCAATAAGGGAGAATTCTCATCGTCTCTCTCAGAGCTGGAATTCGAAAATGCTTCTATCATAGAATTGATGGATTATGATGTTCTTTATCAAACCTTTCAGGGCTGTCATGGAGATCTTACATCAGAAGAAAGACAAATTCTACTGGCGGAGTTCTCTCTTAGGCATAGCAAGAGTGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGCAATCTCTAGTACGGAGGTATTGCTGTAGAATGCCAAAAGTTTTGGGCATTTGATGTGTTGGAAATGTTTACGAGAAGCAGAAACACAGGGAATCTCTTTCAAAGTTTACACTAAAACGGGGCTCCGGGAATTTGCAATGAATTCGAAAGCAGTTTGTAAAGGCTGCGTTCTATCCCAAAAACTTCTACATCAACTCATATGCCTCTGTATAATGTTATTGTAGTTCTCCATTTGACTTGGAGAAACGTTACCTTCGGCTTCAATTGGTAATTTTTTTAAAAAAGAATTTAGCTTATAAATACTATTTTTACCTTC

mRNA sequence

ATGGAATTGTGTTATTTCCTTGTGGTTGGTACCCCTAAAAAAAGATCTTTCTTGTTAGTTCTTAGTAGCTGCTGGGTTGGTTTCTTCTTACCTCTGTCTGAACCGTTGAACCTCTTTCAACGCACATCCATGGCGTCCTGCAGAATCGTATCCCGTTCTTCTCTTTTTCTGCTCCAAAGCTGCAGTCCCCGAAGAACTCAACGGATTTTCTCTTCTGTTTTTCTCCCTTCTCTACACCTTCCCTCCTTCTCTCTGTTCTTCACTAACAGATTTTCTACAAGATGTTTCGCTTCAATTTCCGCCGCAACATCGCCACTAGAAGTAGAAGATATTGTGGCTGACGTAGAACCACCGGAGGTTCGTACACAAGACACGGTGGAGCACCTGCTCACGCATTCCAATGACGTTGCTAGGTTGATGAAGATGGAACGGAGGTCGGTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGTGGTTTCCGTATCTGGATAAGTATAGCTGTGGAAGTTCTTGGTTGAATAGTGGTGAGATTTTGGAGGCCTTGGATTCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTGGTGCGGAATAGAAGCTACTCCGTTTGTTTGGTGGTGGAGGGATTGACAGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCTGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCTACCCCAGAATGTTTTAAAATTCTGAAATCCCGGGGTTATAGAATAGTCTCTACTCATCTAGGAGCGAATGCGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGGGAATAAGTAATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTTTCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCAATCGGACTTCTCGGCTGGGCTGTCATGGAGATCTTACATCAGAAGAAAGACAAATTCTACTGGCGGAGTTCTCTCTTAGGCATAGCAAGAGTGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGCAATCTCTAGTACGGAGGTATTGCTGTAG

Coding sequence (CDS)

ATGGAATTGTGTTATTTCCTTGTGGTTGGTACCCCTAAAAAAAGATCTTTCTTGTTAGTTCTTAGTAGCTGCTGGGTTGGTTTCTTCTTACCTCTGTCTGAACCGTTGAACCTCTTTCAACGCACATCCATGGCGTCCTGCAGAATCGTATCCCGTTCTTCTCTTTTTCTGCTCCAAAGCTGCAGTCCCCGAAGAACTCAACGGATTTTCTCTTCTGTTTTTCTCCCTTCTCTACACCTTCCCTCCTTCTCTCTGTTCTTCACTAACAGATTTTCTACAAGATGTTTCGCTTCAATTTCCGCCGCAACATCGCCACTAGAAGTAGAAGATATTGTGGCTGACGTAGAACCACCGGAGGTTCGTACACAAGACACGGTGGAGCACCTGCTCACGCATTCCAATGACGTTGCTAGGTTGATGAAGATGGAACGGAGGTCGGTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGTGGTTTCCGTATCTGGATAAGTATAGCTGTGGAAGTTCTTGGTTGAATAGTGGTGAGATTTTGGAGGCCTTGGATTCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTGGTGCGGAATAGAAGCTACTCCGTTTGTTTGGTGGTGGAGGGATTGACAGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCTGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCTACCCCAGAATGTTTTAAAATTCTGAAATCCCGGGGTTATAGAATAGTCTCTACTCATCTAGGAGCGAATGCGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGGGAATAAGTAATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTTTCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCAATCGGACTTCTCGGCTGGGCTGTCATGGAGATCTTACATCAGAAGAAAGACAAATTCTACTGGCGGAGTTCTCTCTTAGGCATAGCAAGAGTGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGCAATCTCTAGTACGGAGGTATTGCTGTAG

Protein sequence

MELCYFLVVGTPKKRSFLLVLSSCWVGFFLPLSEPLNLFQRTSMASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSTRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSAISSTEVLL*

Homology

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BLAST of CsaV3_3G041040.1 vs. NCBI nr
Match: XP_011652219.1 (uncharacterized protein LOC101218911 isoform X2 [Cucumis sativus] >KAE8651207.1 hypothetical protein Csa_001530 [Cucumis sativus])

HSP 1 Score: 806.6 bits (2082), Expect = 9.8e-230
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0


Query: 1   MELCYFLVVGTPKKRSFLLVLSSCWVGFFLPLSEPLNLFQRTSMASCRIVSRSSLFLLQS 60
           MELCYFLVVGTPKKRSFLLVLSSCWVGFFLPLSEPLNLFQRTSMASCRIVSRSSLFLLQS
Sbjct: 1   MELCYFLVVGTPKKRSFLLVLSSCWVGFFLPLSEPLNLFQRTSMASCRIVSRSSLFLLQS 60

Query: 61  CSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSTRCFASISAATSPLEVEDIVADVEPPEV 120
           CSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSTRCFASISAATSPLEVEDIVADVEPPEV
Sbjct: 61  CSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSTRCFASISAATSPLEVEDIVADVEPPEV 120

Query: 121 RTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYPHKGRWFPYLDKYSCGSSWLNSGEIL 180
           RTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYPHKGRWFPYLDKYSCGSSWLNSGEIL
Sbjct: 121 RTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYPHKGRWFPYLDKYSCGSSWLNSGEIL 180

Query: 181 EALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCD 240
           EALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCD
Sbjct: 181 EALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCD 240

Query: 241 ASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSC 300
           ASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSC
Sbjct: 241 ASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSC 300

Query: 301 PTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSRLGCH 360
           PTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSRLGCH
Sbjct: 301 PTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSRLGCH 360

Query: 361 GDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSAISSTEVLL 406
           GDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSAISSTEVLL
Sbjct: 361 GDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSAISSTEVLL 405

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BLAST of CsaV3_3G041040.1 vs. NCBI nr
Match: XP_004136796.2 (uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus])

HSP 1 Score: 799.3 bits (2063), Expect = 1.6e-227
Identity = 405/413 (98.06%), Postives = 405/413 (98.06%), Query Frame = 0


Query: 1   MELCYFLVVGTPKKRSFLLVLSSCWVGFFLPLSEPLNLFQRTSMASCRIVSRSSLFLLQS 60
           MELCYFLVVGTPKKRSFLLVLSSCWVGFFLPLSEPLNLFQRTSMASCRIVSRSSLFLLQS
Sbjct: 1   MELCYFLVVGTPKKRSFLLVLSSCWVGFFLPLSEPLNLFQRTSMASCRIVSRSSLFLLQS 60

Query: 61  CSPRRTQRIFSSVFLPSLHLPSFSLFFTNRF--------STRCFASISAATSPLEVEDIV 120
           CSPRRTQRIFSSVFLPSLHLPSFSLFFTNRF        STRCFASISAATSPLEVEDIV
Sbjct: 61  CSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRCFASISAATSPLEVEDIV 120

Query: 121 ADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYPHKGRWFPYLDKYSCGSS 180
           ADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYPHKGRWFPYLDKYSCGSS
Sbjct: 121 ADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYPHKGRWFPYLDKYSCGSS 180

Query: 181 WLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQ 240
           WLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQ
Sbjct: 181 WLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQ 240

Query: 241 SVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVS 300
           SVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVS
Sbjct: 241 SVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVS 300

Query: 301 VYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCN 360
           VYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCN
Sbjct: 301 VYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCN 360

Query: 361 RTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSAISSTEVLL 406
           RTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSAISSTEVLL
Sbjct: 361 RTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSAISSTEVLL 413

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BLAST of CsaV3_3G041040.1 vs. NCBI nr
Match: XP_008443364.1 (PREDICTED: tRNA (guanosine(18)-2'-O)-methyltransferase [Cucumis melo] >KAA0053795.1 tRNA (guanosine(18)-2'-O)-methyltransferase [Cucumis melo var. makuwa] >TYK25606.1 tRNA (guanosine(18)-2'-O)-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 661.8 bits (1706), Expect = 3.9e-186
Identity = 341/370 (92.16%), Postives = 349/370 (94.32%), Query Frame = 0


Query: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRF--------STRC 103
           MASCRIVSRSSL LLQS SPRRTQR+F SVFLPSLHLPS S FFTN          S+RC
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFTNTLSRSNPNAVSSRC 60

Query: 104 FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 163
           FASISAATSPLE+ DIVADVEPPEV TQDTVEHLLTHS+DV+RLMKMERR VELDGAEYP
Sbjct: 61  FASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYP 120

Query: 164 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 223
           HKGRWFPYLDKYS GSSWLNSGEILEALDSLILD+RKERFKNVVRNRSYSVCLVVEGL+D
Sbjct: 121 HKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSD 180

Query: 224 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 283
           FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNST ECFKILK
Sbjct: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECFKILK 240

Query: 284 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 343
           SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300

Query: 344 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 403
           FNVSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 301 FNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360

Query: 404 SAISSTEVLL 406
           SAISST VLL
Sbjct: 361 SAISSTGVLL 369

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BLAST of CsaV3_3G041040.1 vs. NCBI nr
Match: XP_038905163.1 (uncharacterized protein LOC120091275 isoform X3 [Benincasa hispida])

HSP 1 Score: 645.2 bits (1663), Expect = 3.8e-181
Identity = 325/365 (89.04%), Postives = 338/365 (92.60%), Query Frame = 0


Query: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFF---TNRFSTRCFASIS 103
           MASCRI +RSSL LL+SCS RR Q IF SV LPSL L SFS FF   TNR STRCFAS S
Sbjct: 1   MASCRIAARSSLILLESCSSRRPQSIFPSVSLPSLPLSSFSPFFTIVTNRLSTRCFASFS 60

Query: 104 AATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYPHKGRW 163
           AATS +EV D V D EPPE+ TQDTVEHLLTHS+DVARLMKMERR  ELDGAEYPH+GRW
Sbjct: 61  AATSSIEVADAVGDREPPEIHTQDTVEHLLTHSDDVARLMKMERRPFELDGAEYPHRGRW 120

Query: 164 FPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVS 223
           FPYLDK+SCGSSWLNSGEILEALD LILDSRKERFKNVVRNRSYSVCLVVEGL+DFGNVS
Sbjct: 121 FPYLDKFSCGSSWLNSGEILEALDPLILDSRKERFKNVVRNRSYSVCLVVEGLSDFGNVS 180

Query: 224 AAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYR 283
           AAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF++LKSRGYR
Sbjct: 181 AAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFRVLKSRGYR 240

Query: 284 IVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSV 343
           IVSTHLG NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNGMVDSFNVSV
Sbjct: 241 IVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSV 300

Query: 344 AAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSAISS 403
           AAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSKSAIS+ANE AKRKGSAIS 
Sbjct: 301 AAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISVANEFAKRKGSAISG 360

Query: 404 TEVLL 406
           TEVLL
Sbjct: 361 TEVLL 365

[Close]

BLAST of CsaV3_3G041040.1 vs. NCBI nr
Match: XP_038905162.1 (uncharacterized protein LOC120091275 isoform X2 [Benincasa hispida])

HSP 1 Score: 639.4 bits (1648), Expect = 2.1e-179
Identity = 324/373 (86.86%), Postives = 337/373 (90.35%), Query Frame = 0


Query: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT-----------NRFS 103
           MASCRI +RSSL LL+SCS RR Q IF SV LPSL L SFS FFT           N  S
Sbjct: 1   MASCRIAARSSLILLESCSSRRPQSIFPSVSLPSLPLSSFSPFFTIVTNRLSRSNPNAVS 60

Query: 104 TRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGA 163
           TRCFAS SAATS +EV D V D EPPE+ TQDTVEHLLTHS+DVARLMKMERR  ELDGA
Sbjct: 61  TRCFASFSAATSSIEVADAVGDREPPEIHTQDTVEHLLTHSDDVARLMKMERRPFELDGA 120

Query: 164 EYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEG 223
           EYPH+GRWFPYLDK+SCGSSWLNSGEILEALD LILDSRKERFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHRGRWFPYLDKFSCGSSWLNSGEILEALDPLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 224 LTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFK 283
           L+DFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF+
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFR 240

Query: 284 ILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGM 343
           +LKSRGYRIVSTHLG NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNGM
Sbjct: 241 VLKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM 300

Query: 344 VDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAK 403
           VDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSKSAIS+ANE AK
Sbjct: 301 VDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISVANEFAK 360

Query: 404 RKGSAISSTEVLL 406
           RKGSAIS TEVLL
Sbjct: 361 RKGSAISGTEVLL 373

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BLAST of CsaV3_3G041040.1 vs. ExPASy Swiss-Prot
Match: O67577 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trmH PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.2e-23
Identity = 61/195 (31.28%), Postives = 113/195 (57.95%), Query Frame = 0


Query: 183 LDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDAS 242
           ++ L+L+ R +R + V+  R   + +  + + +  N SA  R+ DA+G   ++    +  
Sbjct: 3   MEYLVLEKRLKRLREVLEKRQKDLIVFADNVKNEHNFSAIVRTCDAVGVLYLYYYHAE-G 62

Query: 243 KRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPT 302
           K+ + N  ++ G+ KW+ IE  ++  +     K+RG++IV+T L   +V+  ++D++ PT
Sbjct: 63  KKAKINEGITQGSHKWVFIEKVDNPVQKLLEFKNRGFQIVATWLSKESVNFREVDYTKPT 122

Query: 303 AIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSR-LGCHG 362
            +VVGNE +G+S E +E++D    IPM GM  S NVSVA GI+++ A   R  + +    
Sbjct: 123 VLVVGNELQGVSPEIVEIADKKIVIPMYGMAQSLNVSVATGIILYEAQRQREEKGMYSRP 182

Query: 363 DLTSEERQILLAEFS 377
            L+ EE Q +L +++
Sbjct: 183 SLSEEEIQKILKKWA 196

[Close]

BLAST of CsaV3_3G041040.1 vs. ExPASy Swiss-Prot
Match: P0AGJ3 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334 GN=trmH PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.1e-21
Identity = 69/209 (33.01%), Postives = 108/209 (51.67%), Query Frame = 0


Query: 190 SRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 249
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 250 HVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNE 309
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 310 SRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSR---LGCHGDLTSE 369
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 370 ERQILLAEFSLRHSKSAISIANELAKRKG 396
           E+Q LL E           +  ++AKRKG
Sbjct: 184 EQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of CsaV3_3G041040.1 vs. ExPASy Swiss-Prot
Match: P0AGJ2 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=trmH PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.1e-21
Identity = 69/209 (33.01%), Postives = 108/209 (51.67%), Query Frame = 0


Query: 190 SRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 249
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 250 HVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNE 309
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 310 SRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSR---LGCHGDLTSE 369
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 370 ERQILLAEFSLRHSKSAISIANELAKRKG 396
           E+Q LL E           +  ++AKRKG
Sbjct: 184 EQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of CsaV3_3G041040.1 vs. ExPASy Swiss-Prot
Match: P0AGJ4 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.1e-21
Identity = 69/209 (33.01%), Postives = 108/209 (51.67%), Query Frame = 0


Query: 190 SRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 249
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 250 HVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNE 309
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 310 SRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSR---LGCHGDLTSE 369
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 370 ERQILLAEFSLRHSKSAISIANELAKRKG 396
           E+Q LL E           +  ++AKRKG
Sbjct: 184 EQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of CsaV3_3G041040.1 vs. ExPASy Swiss-Prot
Match: Q72GI1 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=trmH PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.0e-20
Identity = 55/161 (34.16%), Postives = 90/161 (55.90%), Query Frame = 0


Query: 189 DSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 248
           ++R+ R + V+R R   + +++E +    N+SA  R+ DA+G    H V  + +      
Sbjct: 6   EARRRRIEEVLRRRQPDLTVLLENVHKPHNLSAILRTCDAVGVLEAHAV--NPTGGVPTF 65

Query: 249 RHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGN 308
              S G+ KW+ + +     E F+ LK RG+ + +T L  +A    ++D++ PTA++ G 
Sbjct: 66  NETSGGSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFGA 125

Query: 309 ESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHA 350
           E  G+S EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 126 EKWGVSEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

[Close]

BLAST of CsaV3_3G041040.1 vs. ExPASy TrEMBL
Match: A0A0A0LC15 (SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824750 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 9.3e-202
Identity = 362/370 (97.84%), Postives = 362/370 (97.84%), Query Frame = 0


Query: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRF--------STRC 103
           MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRF        STRC
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 60

Query: 104 FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 163
           FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP
Sbjct: 61  FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 120

Query: 164 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 223
           HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD
Sbjct: 121 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 180

Query: 224 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 283
           FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK
Sbjct: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 240

Query: 284 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 343
           SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300

Query: 344 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 403
           FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 301 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360

Query: 404 SAISSTEVLL 406
           SAISSTEVLL
Sbjct: 361 SAISSTEVLL 370

[Close]

BLAST of CsaV3_3G041040.1 vs. ExPASy TrEMBL
Match: A0A5A7UJ80 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007650 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.9e-186
Identity = 341/370 (92.16%), Postives = 349/370 (94.32%), Query Frame = 0


Query: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRF--------STRC 103
           MASCRIVSRSSL LLQS SPRRTQR+F SVFLPSLHLPS S FFTN          S+RC
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFTNTLSRSNPNAVSSRC 60

Query: 104 FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 163
           FASISAATSPLE+ DIVADVEPPEV TQDTVEHLLTHS+DV+RLMKMERR VELDGAEYP
Sbjct: 61  FASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYP 120

Query: 164 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 223
           HKGRWFPYLDKYS GSSWLNSGEILEALDSLILD+RKERFKNVVRNRSYSVCLVVEGL+D
Sbjct: 121 HKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSD 180

Query: 224 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 283
           FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNST ECFKILK
Sbjct: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECFKILK 240

Query: 284 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 343
           SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300

Query: 344 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 403
           FNVSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 301 FNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360

Query: 404 SAISSTEVLL 406
           SAISST VLL
Sbjct: 361 SAISSTGVLL 369

[Close]

BLAST of CsaV3_3G041040.1 vs. ExPASy TrEMBL
Match: A0A1S3B8M3 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486969 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.9e-186
Identity = 341/370 (92.16%), Postives = 349/370 (94.32%), Query Frame = 0


Query: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRF--------STRC 103
           MASCRIVSRSSL LLQS SPRRTQR+F SVFLPSLHLPS S FFTN          S+RC
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFTNTLSRSNPNAVSSRC 60

Query: 104 FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 163
           FASISAATSPLE+ DIVADVEPPEV TQDTVEHLLTHS+DV+RLMKMERR VELDGAEYP
Sbjct: 61  FASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYP 120

Query: 164 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 223
           HKGRWFPYLDKYS GSSWLNSGEILEALDSLILD+RKERFKNVVRNRSYSVCLVVEGL+D
Sbjct: 121 HKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSD 180

Query: 224 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 283
           FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNST ECFKILK
Sbjct: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECFKILK 240

Query: 284 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 343
           SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300

Query: 344 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 403
           FNVSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 301 FNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360

Query: 404 SAISSTEVLL 406
           SAISST VLL
Sbjct: 361 SAISSTGVLL 369

[Close]

BLAST of CsaV3_3G041040.1 vs. ExPASy TrEMBL
Match: A0A6J1HYK4 (uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 4.1e-165
Identity = 305/375 (81.33%), Postives = 330/375 (88.00%), Query Frame = 0


Query: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT---NRFS-------- 103
           MASCRI  R SLFLL SCS R TQ I  SV  PS  L S+S FFT   N  S        
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60

Query: 104 TRCFASISAATSPLEVEDIV-ADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDG 163
           TRCF+S SAA S +EV D V +D+EPPE+ TQDTVEHLLTHS+DVARLMKMERR + LDG
Sbjct: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 164 AEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVE 223
           AEYPH+GRWFPYLDK+SC +SWLNSGE+L+ALD LILDSRKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 224 GLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 283
           GL+DFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 284 KILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 343
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHC+IPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300

Query: 344 MVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 403
           MVDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 404 KRKGSAISS-TEVLL 406
           KRK S+ SS  EVLL
Sbjct: 361 KRKSSSTSSGLEVLL 375

[Close]

BLAST of CsaV3_3G041040.1 vs. ExPASy TrEMBL
Match: A0A6J1F0V5 (uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC111441136 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 5.3e-165
Identity = 304/375 (81.07%), Postives = 328/375 (87.47%), Query Frame = 0


Query: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT-----------NRFS 103
           MASCRI  R SLFLL SCSPR TQRI  SV   S  L S+S FFT              S
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 104 TRCFASISAATSPLEVEDIV-ADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDG 163
            RCF+S SAA S +EV D V +D+EPPE+ TQDTVEHLLTHS+DVARLMKMERR + LDG
Sbjct: 61  IRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 164 AEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVE 223
            EYP +GRWFPYLDK+SC SSWLNSGE+L+ALD LILDSRKERFKNVVR+RSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 224 GLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 283
           GL+DFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 284 KILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 343
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300

Query: 344 MVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 403
           MVDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 404 KRKGSAISS-TEVLL 406
           KRK S+ SS +EVLL
Sbjct: 361 KRKSSSTSSGSEVLL 375

[Close]

BLAST of CsaV3_3G041040.1 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 391.3 bits (1004), Expect = 9.2e-109
Identity = 212/349 (60.74%), Postives = 254/349 (72.78%), Query Frame = 0


Query: 58  LQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSTRCFASISA--------ATSPLEVE 117
           + SC       I S  F+P L  P+FS+ F    S R F+ +S          T+     
Sbjct: 1   MNSCKSLIRASISSFPFVP-LPNPNFSITF---ISVRAFSPLSVLHPNSSCIVTARRTFH 60

Query: 118 DIVADVEP---PEVRTQDTVEHLL-THSNDVARLMKMERRSVELDGAEYPHKGRWFPYLD 177
             VA + P    E   +DTV+ LL T+  + + LMKMERR   L   E   +G WFPY D
Sbjct: 61  GAVA-LSPESLTEESPKDTVKGLLTTNRGEASSLMKMERR-CSLSNGEGDCRGSWFPYED 120

Query: 178 KYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTDFGNVSAAFRS 237
           ++ CG   L+S E+LEA+   +++ R +RF+ VV NRSYSVCLVVEGL+DFGN+SAAFRS
Sbjct: 121 RFRCGEVHLSSREVLEAVSPHMMEERTDRFRRVVENRSYSVCLVVEGLSDFGNISAAFRS 180

Query: 238 ADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTH 297
           ADALG QSVHVVSCD+SKRY  NRHVSMGAEKWLDIE W++  ECFK+LKSRGYRI +TH
Sbjct: 181 ADALGIQSVHVVSCDSSKRYNGNRHVSMGAEKWLDIEFWDTPKECFKVLKSRGYRIATTH 240

Query: 298 LGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGIL 357
           LG + VS+YDMDWSCPTAIVVGNE RGIS+EALELSDL CSIPMNGMVDSFNVSVAAGIL
Sbjct: 241 LGMDTVSIYDMDWSCPTAIVVGNEGRGISDEALELSDLRCSIPMNGMVDSFNVSVAAGIL 300

Query: 358 MHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRK 395
           MHHAV +RT+RLG HGDL+  E++IL+AEFSLRHS+S+I IA E AKRK
Sbjct: 301 MHHAVSDRTTRLGSHGDLSEAEKEILMAEFSLRHSRSSICIAYEFAKRK 343

[Close]

BLAST of CsaV3_3G041040.1 vs. TAIR 10
Match: AT2G19870.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 57.8 bits (138), Expect = 2.4e-08
Identity = 41/173 (23.70%), Postives = 78/173 (45.09%), Query Frame = 0


Query: 204 YSVCLVVEGLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIEL 263
           YS+ + ++ +TD  N+ A  RSA   G   V V + +++         S G+ + +++  
Sbjct: 416 YSLWVALDEVTDPQNLGAIIRSAYFFGATGVVVCAKNSAPLSAVVSKASAGSLEVMELRY 475

Query: 264 WNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRG----ISNEALE 323
             +  +  +     G+R+V   +   AV++ ++    PT +V+GNE  G    +     +
Sbjct: 476 CKNMMQFLEASAENGWRVVGGSVSPKAVALNEVLPGSPTILVLGNEGTGLRPLVERSCTD 535

Query: 324 LSDLHCSIPMNG-----------------MVDSFNVSVAAGILMHHAVCNRTS 356
           L  +  ++P                     V+S NVSVAAG+ +HH + N+ S
Sbjct: 536 LVRISGNMPNEVAVTESDDAEGEGFRSFLAVESLNVSVAAGLFLHHLIGNKAS 588

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BLAST of CsaV3_3G041040.1 vs. TAIR 10
Match: AT4G38020.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 46.6 bits (109), Expect = 5.5e-05
Identity = 42/153 (27.45%), Postives = 74/153 (48.37%), Query Frame = 0


Query: 208 LVVEGLTDFGNVSAAFRSADALGFQSVHVV--SCDA--SKRYRENRHVS----MGAEKWL 267
           LV++ + D GN+    RSA A  +    ++   CD    K  R +R  S    + +  W 
Sbjct: 199 LVLDSIQDPGNLGTLVRSAMAFNWDGAFLLPGCCDPYNDKALRASRGASFQLPIVSGNWN 258

Query: 268 DIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWS-----CPTAIVVGNESRGIS 327
            ++L  +    F++    G+   +T      VS   ++++      P  +++G+E  G+S
Sbjct: 259 HLKLLENE---FQMKLLAGHPATTTQ-KLKPVSKLSVEFAQSLAEKPLCLILGSEGNGLS 318

Query: 328 NEALELSDLHCSIPMNGMVDSFNVSVAAGILMH 348
            +A ++  L  SIPM G  +S NVSVA GI ++
Sbjct: 319 EQARKVCVL-VSIPMAGDFESLNVSVAGGIFLY 346

The following BLAST results are available for this feature:

BLAST of CsaV3_3G041040.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652219.1	9.8e-230	100.00	uncharacterized protein LOC101218911 isoform X2 [Cucumis sativus] >KAE8651207.1 ...	[more]
XP_004136796.2	1.6e-227	98.06	uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus]	[more]
XP_008443364.1	3.9e-186	92.16	PREDICTED: tRNA (guanosine(18)-2'-O)-methyltransferase [Cucumis melo] >KAA005379...	[more]
XP_038905163.1	3.8e-181	89.04	uncharacterized protein LOC120091275 isoform X3 [Benincasa hispida]	[more]
XP_038905162.1	2.1e-179	86.86	uncharacterized protein LOC120091275 isoform X2 [Benincasa hispida]	[more]

BLAST of CsaV3_3G041040.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O67577	1.2e-23	31.28	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=...	[more]
P0AGJ3	1.1e-21	33.01	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334...	[more]
P0AGJ2	1.1e-21	33.01	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=...	[more]
P0AGJ4	1.1e-21	33.01	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH ...	[more]
Q72GI1	2.0e-20	34.16	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC...	[more]

BLAST of CsaV3_3G041040.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LC15	9.3e-202	97.84	SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824...	[more]
A0A5A7UJ80	1.9e-186	92.16	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B8M3	1.9e-186	92.16	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486...	[more]
A0A6J1HYK4	4.1e-165	81.33	uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788...	[more]
A0A6J1F0V5	5.3e-165	81.07	uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC1114411...	[more]

BLAST of CsaV3_3G041040.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15390.1	9.2e-109	60.74	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT2G19870.1	2.4e-08	23.70	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT4G38020.1	5.5e-05	27.45	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 206..347 e-value: 8.3E-35 score: 120.0
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 182..388 e-value: 9.1E-60 score: 203.4
None	No IPR available	PIRSR	PIRSR029256-1	PIRSR029256-1	coord: 258..349 e-value: 9.7E-5 score: 19.9
None	No IPR available	PANTHER	PTHR43453:SF1	RRNA METHYLASE-LIKE PROTEIN	coord: 44..398
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	PANTHER	PTHR43453	RRNA METHYLASE-LIKE	coord: 44..398
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	HAMAP	MF_02060	tRNA_methyltr_TrmH	coord: 188..382 score: 26.93264
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	CDD	cd18092	SpoU-like_TrmH	coord: 190..349 e-value: 8.23595E-79 score: 238.521
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 189..357

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CsaV3_3G041040	CsaV3_3G041040	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsaV3_3G041040.1.exon1	CsaV3_3G041040.1.exon1	exon
CsaV3_3G041040.1.exon2	CsaV3_3G041040.1.exon2	exon
CsaV3_3G041040.1.exon3	CsaV3_3G041040.1.exon3	exon
CsaV3_3G041040.1.exon4	CsaV3_3G041040.1.exon4	exon
CsaV3_3G041040.1.exon5	CsaV3_3G041040.1.exon5	exon
CsaV3_3G041040.1.exon6	CsaV3_3G041040.1.exon6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsaV3_3G041040.1.cds1	CsaV3_3G041040.1.cds1	CDS
CsaV3_3G041040.1.cds2	CsaV3_3G041040.1.cds2	CDS
CsaV3_3G041040.1.cds3	CsaV3_3G041040.1.cds3	CDS
CsaV3_3G041040.1.cds4	CsaV3_3G041040.1.cds4	CDS
CsaV3_3G041040.1.cds5	CsaV3_3G041040.1.cds5	CDS
CsaV3_3G041040.1.cds6	CsaV3_3G041040.1.cds6	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CsaV3_3G041040.1	CsaV3_3G041040.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006396	RNA processing
biological_process	GO:0030488	tRNA methylation
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity