CsGy6G006340.1 (mRNA) Cucumber (Gy14) v2.1

Overview


Name	CsGy6G006340.1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	PMEI domain-containing protein
Location	Gy14Chr6: 5532807 .. 5533373 (-)
Sequence length	567
RNA-Seq Expression	CsGy6G006340.1
Synteny	CsGy6G006340.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATACCTCATTATTCATCTGTTTTTATTCTCTCAGTTTTCTTCTTCTCCAACTTCCTCATTATACAATCCTCCAAAACCATCAAAACAGCTGATCTAATCTACAAAACTTGCAAGAAAATCTCAAGAGAAGACCCTAACGTTTCCTTCAACTTTTGCTTAGCATCTCTAAAACTTGCCACCAACCATAGCCGATGCACCGACGTTCGCCACCTCGGCCTATTCTCGATTGGGTTCTTATGTAGGAATGTGACCAGCACGTACCACCATATCACAAAGTTGGTGAGGAACAAGAAGCTCGACCCTTTTGTTAAGTTATGTTTGGATGATTGTTTGGAGCTTTATACTGATGCGATACCAACGGTGAAGCAAGCGATGAAGGATTATAAGTCGAAGAGATATGACGATGCGAATGTAGCTATTAGTTCGGTGATGGATGCAGCAACGACTTGTGAGGATGGGTTTAAGGAGAGGAAAGGGGTAGCTTCACCATTGAAGAAGAGGGATGGAGATGCTTTTGAATTGGGAGCCATTGCATTGTCAATTATGAGTTTGCTTGGTTGA

mRNA sequence

ATGGCCATACCTCATTATTCATCTGTTTTTATTCTCTCAGTTTTCTTCTTCTCCAACTTCCTCATTATACAATCCTCCAAAACCATCAAAACAGCTGATCTAATCTACAAAACTTGCAAGAAAATCTCAAGAGAAGACCCTAACGTTTCCTTCAACTTTTGCTTAGCATCTCTAAAACTTGCCACCAACCATAGCCGATGCACCGACGTTCGCCACCTCGGCCTATTCTCGATTGGGTTCTTATGTAGGAATGTGACCAGCACGTACCACCATATCACAAAGTTGGTGAGGAACAAGAAGCTCGACCCTTTTGTTAAGTTATGTTTGGATGATTGTTTGGAGCTTTATACTGATGCGATACCAACGGTGAAGCAAGCGATGAAGGATTATAAGTCGAAGAGATATGACGATGCGAATGTAGCTATTAGTTCGGTGATGGATGCAGCAACGACTTGTGAGGATGGGTTTAAGGAGAGGAAAGGGGTAGCTTCACCATTGAAGAAGAGGGATGGAGATGCTTTTGAATTGGGAGCCATTGCATTGTCAATTATGAGTTTGCTTGGTTGA

Coding sequence (CDS)

ATGGCCATACCTCATTATTCATCTGTTTTTATTCTCTCAGTTTTCTTCTTCTCCAACTTCCTCATTATACAATCCTCCAAAACCATCAAAACAGCTGATCTAATCTACAAAACTTGCAAGAAAATCTCAAGAGAAGACCCTAACGTTTCCTTCAACTTTTGCTTAGCATCTCTAAAACTTGCCACCAACCATAGCCGATGCACCGACGTTCGCCACCTCGGCCTATTCTCGATTGGGTTCTTATGTAGGAATGTGACCAGCACGTACCACCATATCACAAAGTTGGTGAGGAACAAGAAGCTCGACCCTTTTGTTAAGTTATGTTTGGATGATTGTTTGGAGCTTTATACTGATGCGATACCAACGGTGAAGCAAGCGATGAAGGATTATAAGTCGAAGAGATATGACGATGCGAATGTAGCTATTAGTTCGGTGATGGATGCAGCAACGACTTGTGAGGATGGGTTTAAGGAGAGGAAAGGGGTAGCTTCACCATTGAAGAAGAGGGATGGAGATGCTTTTGAATTGGGAGCCATTGCATTGTCAATTATGAGTTTGCTTGGTTGA

Protein sequence

MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLLG*

Homology

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BLAST of CsGy6G006340.1 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 5.9e-31
Identity = 72/179 (40.22%), Postives = 119/179 (66.48%), Query Frame = 0


Query: 10  FILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTD 69
           F L +FFF+  L++Q+   + +AD++  TCKK+++  PNV+++FC+ SL  A   S   D
Sbjct: 5   FSLCIFFFNLLLLLQA---VISADIVQGTCKKVAQRSPNVNYDFCVKSLG-ADPKSHTAD 64

Query: 70  VRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKD 129
           ++ LG+ S     ++ +     I +++++ K+DP +K  L+DC+ LY DA  +V++A+ D
Sbjct: 65  LQGLGVISANLAIQHGSKIQTFIGRILKS-KVDPALKKYLNDCVGLYADAKSSVQEAIAD 124

Query: 130 YKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLLG 189
           +KSK Y  ANV +S+ +D + TCEDGFKE+KG+ SP+ K + D  +L AI+L+I  LLG
Sbjct: 125 FKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKLLG 178

[Close]

BLAST of CsGy6G006340.1 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 3.3e-29
Identity = 66/158 (41.77%), Postives = 106/158 (67.09%), Query Frame = 0


Query: 31  TADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTDVRHLGLFSIGFLCRNVTSTYH 90
           +AD++  TCKK+++  PNV+++FC+ SL  A   S   D++ LG+ S     +  +    
Sbjct: 14  SADIVQGTCKKVAQRSPNVNYDFCVKSLG-ADPKSHSADLQGLGVISANLAIQQGSKIQT 73

Query: 91  HITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKDYKSKRYDDANVAISSVMDAAT 150
            I +++++ K+DP +K  L+DC+ LY DA  +V++A+ D+KSK Y  ANV +S+ +D + 
Sbjct: 74  FIGRILKS-KVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSV 133

Query: 151 TCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLLG 189
           TCEDGFKE+KG+ASP+ K + D  +L AI+L+I  LLG
Sbjct: 134 TCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKLLG 169

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BLAST of CsGy6G006340.1 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.7e-12
Identity = 53/178 (29.78%), Postives = 88/178 (49.44%), Query Frame = 0


Query: 10  FILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTD 69
           F++S+  FS FL      T +T  LI  +CKK   +DP +S++FC+ SL         T 
Sbjct: 3   FLVSLVIFSLFL--NGFATAQT--LIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATT 62

Query: 70  VRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKD 129
           +  L L S       +T+    + + +++++    V+  L  CL  Y DA   +  A+ +
Sbjct: 63  LESLVLASTKTAAAKITNLKGIVAQDLKDQRYQDIVE-DLKLCLGFYNDANDDLTTALAN 122

Query: 130 YKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 188
            KS+ Y  AN+ +S+ +D    CED FKE K   SP+   +   F+   I L+  ++L
Sbjct: 123 IKSRDYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNML 174

[Close]

BLAST of CsGy6G006340.1 vs. NCBI nr
Match: XP_004145026.1 (putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 371 bits (952), Expect = 2.09e-129
Identity = 187/188 (99.47%), Postives = 187/188 (99.47%), Query Frame = 0


Query: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL
Sbjct: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           A NHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI
Sbjct: 61  AANHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
           PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA
Sbjct: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180

Query: 181 LSIMSLLG 188
           LSIMSLLG
Sbjct: 181 LSIMSLLG 188

[Close]

BLAST of CsGy6G006340.1 vs. NCBI nr
Match: KAE8646718.1 (hypothetical protein Csa_005009 [Cucumis sativus])

HSP 1 Score: 349 bits (895), Expect = 5.13e-119
Identity = 175/180 (97.22%), Postives = 177/180 (98.33%), Query Frame = 0


Query: 9   VFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCT 68
           VF  ++FFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLA NHSRCT
Sbjct: 121 VFCKAIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLAANHSRCT 180

Query: 69  DVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMK 128
           DVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMK
Sbjct: 181 DVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMK 240

Query: 129 DYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLLG 188
           DYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLLG
Sbjct: 241 DYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLLG 300

[Close]

BLAST of CsGy6G006340.1 vs. NCBI nr
Match: XP_008460048.1 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 338 bits (866), Expect = 2.70e-116
Identity = 170/187 (90.91%), Postives = 176/187 (94.12%), Query Frame = 0


Query: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAIPHY SVFILS+FFFSNFLIIQSSKTIKTADLIYKTCKKISREDPN+SFNFCL SLKL
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           ATNHSRCTDVRHLGL SIG L RNVTST HHITKLV+NKKLDPFVK CLDDCLELY+DAI
Sbjct: 61  ATNHSRCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
           PTVKQAMKDYKSKRYDD NVAI SVMDAATTCEDGFKERKGVASPLKKRD +AFELGAI 
Sbjct: 121 PTVKQAMKDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAIV 180

Query: 181 LSIMSLL 187
           LSIMSL+
Sbjct: 181 LSIMSLV 187

[Close]

BLAST of CsGy6G006340.1 vs. NCBI nr
Match: KAA0039956.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK24547.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 337 bits (863), Expect = 7.75e-116
Identity = 169/187 (90.37%), Postives = 176/187 (94.12%), Query Frame = 0


Query: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAIPHY SVFILS+FFFSNFLIIQSSKTIKTADLIYKTCKKISREDPN+SFNFCL SLKL
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           ATNHSRCTDVRHLGL SIG L RNVTST HHITKLV+NKKLDPFVK CLDDCLELY+DAI
Sbjct: 61  ATNHSRCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
           PTVKQAM+DYKSKRYDD NVAI SVMDAATTCEDGFKERKGVASPLKKRD +AFELGAI 
Sbjct: 121 PTVKQAMRDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAIV 180

Query: 181 LSIMSLL 187
           LSIMSL+
Sbjct: 181 LSIMSLV 187

[Close]

BLAST of CsGy6G006340.1 vs. NCBI nr
Match: XP_038875573.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 268 bits (684), Expect = 1.44e-88
Identity = 137/187 (73.26%), Postives = 153/187 (81.82%), Query Frame = 0


Query: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAI  Y S FILS+FFFSNFLI QSS TI T DLI +TC KISREDPN+S++FCL SL+ 
Sbjct: 1   MAISLYFSAFILSIFFFSNFLITQSSITINTTDLIQETCMKISREDPNISYDFCLTSLES 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           AT    C ++RHLGL SIG L RNVTST HHI  L+ N KL PFVK CL+DCLELY+DA+
Sbjct: 61  ATKDIECINLRHLGLISIGLLRRNVTSTRHHIISLMENTKLYPFVKFCLEDCLELYSDAV 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
           PTV QAM DYK K+YDDAN+A+S VMDAA TCEDGFKERKGV SPLKKRD DAFELGAIA
Sbjct: 121 PTVTQAMWDYKLKQYDDANIAVSLVMDAAETCEDGFKERKGVVSPLKKRDEDAFELGAIA 180

Query: 181 LSIMSLL 187
           LSI+SLL
Sbjct: 181 LSIISLL 187

[Close]

BLAST of CsGy6G006340.1 vs. ExPASy TrEMBL
Match: A0A0A0K951 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080380 PE=4 SV=1)

HSP 1 Score: 371 bits (952), Expect = 1.01e-129
Identity = 187/188 (99.47%), Postives = 187/188 (99.47%), Query Frame = 0


Query: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL
Sbjct: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           A NHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI
Sbjct: 61  AANHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
           PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA
Sbjct: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180

Query: 181 LSIMSLLG 188
           LSIMSLLG
Sbjct: 181 LSIMSLLG 188

[Close]

BLAST of CsGy6G006340.1 vs. ExPASy TrEMBL
Match: A0A1S3CBQ9 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103498981 PE=4 SV=1)

HSP 1 Score: 338 bits (866), Expect = 1.31e-116
Identity = 170/187 (90.91%), Postives = 176/187 (94.12%), Query Frame = 0


Query: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAIPHY SVFILS+FFFSNFLIIQSSKTIKTADLIYKTCKKISREDPN+SFNFCL SLKL
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           ATNHSRCTDVRHLGL SIG L RNVTST HHITKLV+NKKLDPFVK CLDDCLELY+DAI
Sbjct: 61  ATNHSRCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
           PTVKQAMKDYKSKRYDD NVAI SVMDAATTCEDGFKERKGVASPLKKRD +AFELGAI 
Sbjct: 121 PTVKQAMKDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAIV 180

Query: 181 LSIMSLL 187
           LSIMSL+
Sbjct: 181 LSIMSLV 187

[Close]

BLAST of CsGy6G006340.1 vs. ExPASy TrEMBL
Match: A0A5A7TCY0 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00920 PE=4 SV=1)

HSP 1 Score: 337 bits (863), Expect = 3.75e-116
Identity = 169/187 (90.37%), Postives = 176/187 (94.12%), Query Frame = 0


Query: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAIPHY SVFILS+FFFSNFLIIQSSKTIKTADLIYKTCKKISREDPN+SFNFCL SLKL
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           ATNHSRCTDVRHLGL SIG L RNVTST HHITKLV+NKKLDPFVK CLDDCLELY+DAI
Sbjct: 61  ATNHSRCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
           PTVKQAM+DYKSKRYDD NVAI SVMDAATTCEDGFKERKGVASPLKKRD +AFELGAI 
Sbjct: 121 PTVKQAMRDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAIV 180

Query: 181 LSIMSLL 187
           LSIMSL+
Sbjct: 181 LSIMSLV 187

[Close]

BLAST of CsGy6G006340.1 vs. ExPASy TrEMBL
Match: A0A6J1KH00 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494271 PE=4 SV=1)

HSP 1 Score: 235 bits (600), Expect = 3.83e-76
Identity = 126/187 (67.38%), Postives = 150/187 (80.21%), Query Frame = 0


Query: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAI  +S +FILS+FFFSNFLI QS   I T  LI+KTC KI+ +DPNVSFNFCL SL+ 
Sbjct: 1   MAISPFS-LFILSIFFFSNFLITQSFSNIDTP-LIHKTCNKIAHQDPNVSFNFCLTSLQF 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           AT HS   D+R LGL S+G +C NVT+TYH IT+L+RNKKLD  VKLCL DCLELY+DAI
Sbjct: 61  ATKHSYYVDLRRLGLLSMGLVCSNVTNTYHFITELIRNKKLDSSVKLCLADCLELYSDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
            TVK AM++Y+ KRY + NVAIS+VM+AA TCE+GFKE+K V SPLKKRD D FELGAI 
Sbjct: 121 STVKLAMRNYRLKRYKNDNVAISAVMNAAETCENGFKEKK-VNSPLKKRDEDVFELGAIV 180

Query: 181 LSIMSLL 187
           L+IMS+L
Sbjct: 181 LAIMSML 184

[Close]

BLAST of CsGy6G006340.1 vs. ExPASy TrEMBL
Match: A0A6J1HN05 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111464420 PE=4 SV=1)

HSP 1 Score: 234 bits (598), Expect = 7.71e-76
Identity = 125/187 (66.84%), Postives = 149/187 (79.68%), Query Frame = 0


Query: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAI  +S +FILS+FFFSNFLI +S     T  LI+KTC KI R+DPNVSFNFCL SL+ 
Sbjct: 1   MAISPFS-LFILSIFFFSNFLITRSVSNTDTP-LIHKTCNKIVRQDPNVSFNFCLTSLQF 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           A  HS   D+R LGL SIG +C NVT+TYH IT+L+RNK LD  +KLCL DCLELY+DAI
Sbjct: 61  AIKHSYYVDLRRLGLLSIGLVCSNVTNTYHFITELIRNKNLDSSIKLCLVDCLELYSDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
            TVK AM++Y+ +RY+DANVAIS+VMDAA TCE+GFKE+K V SPLKKRD D FELGAI 
Sbjct: 121 STVKLAMRNYRLRRYNDANVAISAVMDAAETCENGFKEKK-VNSPLKKRDEDVFELGAIV 180

Query: 181 LSIMSLL 187
           L+IMS+L
Sbjct: 181 LAIMSML 184

[Close]

BLAST of CsGy6G006340.1 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.1e-19
Identity = 49/154 (31.82%), Postives = 82/154 (53.25%), Query Frame = 0


Query: 34  LIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHIT 93
           LI  +CKK +   P   +N C+ SL+         D+  L + S        T+    + 
Sbjct: 23  LIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLVMASTKNAVTKATTLKGTVD 82

Query: 94  KLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKDYKSKRYDDANVAISSVMDAATTCE 153
           K+++ KK++    + L DCL+LYTDAI ++ +A+   KS+ Y      +S+ MD  +TCE
Sbjct: 83  KIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRNYPTVKTVLSAAMDTPSTCE 142

Query: 154 DGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 188
            GFKERK   SP+ K + + +++  I L+  ++L
Sbjct: 143 TGFKERK-APSPVTKENDNLYQMILIPLAFTNML 175

[Close]

BLAST of CsGy6G006340.1 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 7.7e-18
Identity = 52/156 (33.33%), Postives = 86/156 (55.13%), Query Frame = 0


Query: 14  VFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTDVRHL 73
           +F+F+ F ++ ++ TI    LI   CKK + ++P + +NFC+ SL+          +  L
Sbjct: 4   LFYFAMFFLLLNAFTI-AQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRL 63

Query: 74  GLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKDYKSK 133
            + S        TS    + K+++  + + + +  L DCLELY+DA  ++K+A+   KS+
Sbjct: 64  VMLSTKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDATNSLKEALTIIKSR 123

Query: 134 RYDDANVAISSVMDAATTCEDGFKE-RKGVASPLKK 169
            Y  ANV IS+ M A  +CE GFKE +K + SP  K
Sbjct: 124 DYKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTK 158

[Close]

BLAST of CsGy6G006340.1 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.9e-17
Identity = 64/192 (33.33%), Postives = 98/192 (51.04%), Query Frame = 0


Query: 12  LSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRC---- 71
           LS  F+  FL    S+   T+D+I +TCK  + +     +NFC++SL    N+S      
Sbjct: 3   LSQVFYIIFLFFLVSQVKSTSDMIDQTCKSCAAKSTIFDYNFCVSSL----NNSPIALPS 62

Query: 72  -TDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQA 131
            T++  L L  +     N T+T   I +L+ +     F   CL DCLELY DA   +++A
Sbjct: 63  PTNLSSLALVPMLQALDNATATASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEA 122

Query: 132 MKDY-KSKRYDDANVAISSVMDAATTCEDGFKERK-------GV---ASPLKKRDGDAFE 188
           ++ +   K     NV +S+ M++A TCE+GF+ER        GV    SP+   +   FE
Sbjct: 123 VRVFITRKELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFE 182

[Close]

BLAST of CsGy6G006340.1 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 9.4e-16
Identity = 54/176 (30.68%), Postives = 91/176 (51.70%), Query Frame = 0


Query: 13  SVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTDVRH 72
           S++    FL++    T ++  LI  +CKK + +DP + ++FC+ SL+          +  
Sbjct: 4   SLYLVMFFLLLNCFATAQS--LIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEG 63

Query: 73  LGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKDYKS 132
           L   S        TS    + K+++  K +  V+  L DCLELYT+AI ++ Q++   KS
Sbjct: 64  LVFVSTKNAVSKTTSLKGMVDKILKEDKYE--VERPLLDCLELYTEAIDSLNQSLDTVKS 123

Query: 133 KRYDDANVAISSVMDAATTCEDGF-KERKGVASPLKKRDGDAFELGAIALSIMSLL 188
           + Y  A + +S+ MDA  +CE  F K +K V SP  K +   F +  I ++  S+L
Sbjct: 124 RDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSML 175

[Close]

BLAST of CsGy6G006340.1 vs. TAIR 10
Match: AT5G46950.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.6e-13
Identity = 53/178 (29.78%), Postives = 91/178 (51.12%), Query Frame = 0


Query: 10  FILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTD 69
           F++S+  FS FL      T +T  LI  +CKK   +DP  S++FC+ SL         T 
Sbjct: 3   FLVSLVIFSLFL--NGFATAQT--LIQDSCKKAFAKDPQSSYDFCVQSLTQDPQSKAATT 62

Query: 70  VRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKD 129
           +  L L S   +   +T+    + + +++++    V+  L  CL  Y DA  ++K A+ +
Sbjct: 63  LEDLALASTKNVAAKITNLKGIVAQDLKDQRYQDIVE-DLKLCLGFYKDANDSLKTALAN 122

Query: 130 YKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 188
            KS+ YD AN  +S+ ++A   CED FKE +   SP+   +   ++   I L+  ++L
Sbjct: 123 IKSRDYDGANSNLSAALNAPGDCEDDFKEAE-KKSPITNENNILYKTIVIPLAFTTML 174

The following BLAST results are available for this feature:

BLAST of CsGy6G006340.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	5.9e-31	40.22	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	3.3e-29	41.77	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	4.7e-12	29.78	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]

BLAST of CsGy6G006340.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145026.1	2.09e-129	99.47	putative invertase inhibitor [Cucumis sativus]	[more]
KAE8646718.1	5.13e-119	97.22	hypothetical protein Csa_005009 [Cucumis sativus]	[more]
XP_008460048.1	2.70e-116	90.91	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
KAA0039956.1	7.75e-116	90.37	putative invertase inhibitor [Cucumis melo var. makuwa] >TYK24547.1 putative inv...	[more]
XP_038875573.1	1.44e-88	73.26	putative invertase inhibitor [Benincasa hispida]	[more]

BLAST of CsGy6G006340.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K951	1.01e-129	99.47	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080380 PE=4 S...	[more]
A0A1S3CBQ9	1.31e-116	90.91	putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103498981 PE=4 SV=1	[more]
A0A5A7TCY0	3.75e-116	90.37	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A6J1KH00	3.83e-76	67.38	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494271 PE=4 SV...	[more]
A0A6J1HN05	7.71e-76	66.84	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111464420 PE=4 ...	[more]

BLAST of CsGy6G006340.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46940.1	1.1e-19	31.82	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	7.7e-18	33.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	2.9e-17	33.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	9.4e-16	30.68	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46950.1	2.6e-13	29.78	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..183 e-value: 3.0E-26 score: 103.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..185 e-value: 1.9E-34 score: 116.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 33..183 e-value: 5.5E-23 score: 82.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 30..187 e-value: 1.8E-33 score: 117.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..187
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 28..187
None	No IPR available	PANTHER	PTHR31080:SF226	PECTINESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN-RELATED	coord: 28..187
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 39..183 e-value: 1.7826E-37 score: 124.785

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CsGy6G006340	CsGy6G006340	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy6G006340.1.exon1	CsGy6G006340.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.CsGy6G006340.1	cds.CsGy6G006340.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CsGy6G006340.1	CsGy6G006340.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity