CsGy3G031120.2 (mRNA) Cucumber (Gy14) v2.1

BLAST of CsGy3G031120.2 vs. ExPASy Swiss-Prot
Match: M4IQS1 (Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 3.0e-208
Identity = 360/543 (66.30%), Postives = 447/543 (82.32%), Query Frame = 0

Query: 5   MNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLTFRQL 64
           M K FNP++Q+Y+S RPP++FPTDP +S+ SFLFR+S+SYPN  AL+DADSG++LTF +L
Sbjct: 1   MEKCFNPETQIYSSHRPPVNFPTDPKLSLTSFLFRSSASYPNRTALIDADSGQTLTFLKL 60

Query: 65  QIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSAELSK 124
           + QVSKLAH  +QL I+K DVVLIFAPNSIHF VCFF+I A+GAI TTCNP+YT  ELS 
Sbjct: 61  KTQVSKLAHSLVQLNIKKNDVVLIFAPNSIHFPVCFFSIAALGAITTTCNPSYTFTELSN 120

Query: 125 QVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKF-SRSNIWAYSDLIKKAGDVSNL 184
           Q  +C P LVITVPELW+   KLNLP+IIL S  +SK  S+S  W +SDL +K+   S L
Sbjct: 121 QAKDCNPTLVITVPELWEKARKLNLPAIILPSPSNSKLSSKSEFWFFSDLTRKSDRFSEL 180

Query: 185 PVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCFLPM 244
           P+S+V Q+DVAALLYSSGTTG SKGV+L+H+NFIT SLMVT DQ+  GDP+N+ +CFLPM
Sbjct: 181 PISDVRQSDVAALLYSSGTTGKSKGVVLSHKNFITTSLMVTSDQDRYGDPQNICMCFLPM 240

Query: 245 FHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK-VV 304
           FH+FGLS++  SQL+RGN VVSM KFELE AL  V  Y++THL+VVPPV+IAL K+  VV
Sbjct: 241 FHIFGLSVIAYSQLRRGNGVVSMGKFELEGALRAVEMYRVTHLFVVPPVMIALAKESVVV 300

Query: 305 KNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVESTQS 364
           + YDLSS+++ILSGAAPLGK+VM++C++ +P A IIQGYGMTETCG+IS+E+       S
Sbjct: 301 RRYDLSSVKEILSGAAPLGKNVMEQCARNVPGAAIIQGYGMTETCGIISIEDSKKGCRFS 360

Query: 365 GATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH 424
           G+TG L  G+E+QI+  +    LPP + GEI +RGPNMM+GY NN +AT  TID+QGWV 
Sbjct: 361 GSTGMLAPGIESQIMDTKRWNPLPPNQEGEIWLRGPNMMQGYLNNLEATKSTIDEQGWVK 420

Query: 425 TGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAGE 484
           TGDIGYF+EEG+LFVVDR+KELIKCYGFQVAPAELEALLLSHP+I DA+VIP PD+KAGE
Sbjct: 421 TGDIGYFDEEGQLFVVDRLKELIKCYGFQVAPAELEALLLSHPEILDAVVIPFPDEKAGE 480

Query: 485 VPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQVR 544
           VPIA VVRSPNSS+SEEDV+ F+  QVAPFK+LR+VTF SSV KS +GK+LRRE+I +VR
Sbjct: 481 VPIANVVRSPNSSLSEEDVQRFIEKQVAPFKKLRRVTFVSSVLKSPAGKILRRELIQKVR 540

Query: 545 AKM 546
           +K+
Sbjct: 541 SKI 543

BLAST of CsGy3G031120.2 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 7.1e-194
Identity = 342/544 (62.87%), Postives = 433/544 (79.60%), Query Frame = 0

Query: 5   MNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLTFRQL 64
           M KS   +  V+ S RPP+  P D  +S+VSF+FRNSSSYP   AL+D+D+ E+L+F Q 
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPKDHNLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQF 60

Query: 65  QIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSAELSK 124
           +  V K++H F+ LG+QK DVVLIFAPNSIH  VCF  IVA GAIATT NP YT +ELSK
Sbjct: 61  KSMVIKVSHGFLNLGVQKNDVVLIFAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSK 120

Query: 125 QVANCKPKLVITVPELWDVIGKLNLPSIILG--SKISSKF-SRSNIWAYSDLIKKAGDVS 184
           QV +  PKL++TVPEL++ +   NLP+I++G  S+ SS   SR+ +  + DL+  +G VS
Sbjct: 121 QVKDSNPKLIVTVPELFEKVKGFNLPTILIGPNSEDSSPLKSRAKVLTFHDLVTLSGPVS 180

Query: 185 NLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCFL 244
           + P+ +  Q+D AALLYSSGTTG+SKGV+L+H+NFI +SLMVT +Q+  G+  NVFLCFL
Sbjct: 181 DFPMVDFKQSDTAALLYSSGTTGMSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLCFL 240

Query: 245 PMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQKV 304
           PMFHVFGL+I+  +QLQRGNTV+SMA+F+LEK L  V KYK+THL+VVPPVI+ALTK  +
Sbjct: 241 PMFHVFGLAIITYAQLQRGNTVISMARFDLEKILKDVEKYKVTHLWVVPPVILALTKNSI 300

Query: 305 VKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVESTQ 364
           VK YDLSSL+ I SGAAPLGKD+M+EC+KI+P   + QGYGMTETCG++SVE+       
Sbjct: 301 VKKYDLSSLKHIGSGAAPLGKDLMEECAKIVPHGIVAQGYGMTETCGIVSVEDTRGGKRH 360

Query: 365 SGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWV 424
           +G+ G L SGVEAQI+S++T K LPP + GEI VRGPNMM+GYFNN +AT  TID +GWV
Sbjct: 361 TGSAGMLSSGVEAQIVSVDTLKPLPPNQLGEIWVRGPNMMQGYFNNPRATKLTIDKKGWV 420

Query: 425 HTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAG 484
           HTGD+GYF+E+G L+VVDRIKELIK  GFQVAPAELE LL+SHP+I DA+VIP PD  AG
Sbjct: 421 HTGDLGYFDEDGHLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDADAG 480

Query: 485 EVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQV 544
           EVP+A+VVRSPNSS++E+DVK F+AGQVA FKRLRKVTF +SVPKSASGK+LRRE+I +V
Sbjct: 481 EVPVAYVVRSPNSSLTEDDVKKFIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQKV 540

Query: 545 RAKM 546
           R+ +
Sbjct: 541 RSNI 544

BLAST of CsGy3G031120.2 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 9.9e-188
Identity = 331/544 (60.85%), Postives = 424/544 (77.94%), Query Frame = 0

Query: 5   MNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLTFRQL 64
           M KS   +  +Y S RP +  P DP  S+VSFLFRNSSSYP+ LA+ D+D+G+SLTF QL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 65  QIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSAELSK 124
           +  V++LAH F +LGI+K DVVLIFAPNS  F +CF A+ AIG + TT NP YT  E+SK
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 125 QVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKF---SRSNIWAYSDLIKKAGDVS 184
           Q+ +  PK++I+V +L+D I   +LP ++LGSK + +    S S I ++ ++++ +  VS
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVS 180

Query: 185 NLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCFL 244
             P  E+ Q+D AALLYSSGTTG SKGV LTH NFI ASLMVT DQ+L+G+   VFLCFL
Sbjct: 181 EYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFL 240

Query: 245 PMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQKV 304
           PMFHVFGL+++  SQLQRGN +VSMA+FELE  L  + K+++THL+VVPPV +AL+KQ +
Sbjct: 241 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 300

Query: 305 VKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVESTQ 364
           VK +DLSSL+ I SGAAPLGKD+M+EC + IP   ++QGYGMTETCG++SVE+  +    
Sbjct: 301 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRN 360

Query: 365 SGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWV 424
           SG+ G L  GVEAQI+S+ET K  PP + GEI VRGPNMMKGY NN +AT +TID + WV
Sbjct: 361 SGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWV 420

Query: 425 HTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAG 484
           HTGD+GYFNE+G L+VVDRIKELIK  GFQVAPAELE LL+SHP I DA+VIP PD++AG
Sbjct: 421 HTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAG 480

Query: 485 EVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQV 544
           EVPIAFVVRSPNSSI+E+D++ F+A QVAP+KRLR+V+F S VPKSA+GK+LRRE++ QV
Sbjct: 481 EVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQV 540

Query: 545 RAKM 546
           R+KM
Sbjct: 541 RSKM 544

BLAST of CsGy3G031120.2 vs. ExPASy Swiss-Prot
Match: Q0DV32 (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 552.0 bits (1421), Expect = 7.7e-156
Identity = 293/538 (54.46%), Postives = 391/538 (72.68%), Query Frame = 0

Query: 15  VYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADS-GESLTFRQLQIQV-SKLA 74
           VY S RPP    +DP +S+   L R + + P+A+AL DA + G +LTF +L+  V S   
Sbjct: 16  VYRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRSAVLSTAV 75

Query: 75  HVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSAELSKQVANCKPK 134
            +  + G++ GD VL+ APN + + VCFFA+ A+GA+ TT NP YT  E++KQV++ + K
Sbjct: 76  ALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQVSDARAK 135

Query: 135 LVITVPELWDVIGKLNLPSIILGSKISSKFSR----SNIWAYSDLIK--KAGDVSNLPVS 194
           LVIT+  L   I  L LP I+L    ++  +     + +  Y++L+   K  D    P+ 
Sbjct: 136 LVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLVAGVKEADYRRPPIK 195

Query: 195 EVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCFLPMFHV 254
              Q+D AALLYSSGTTG SKGVILTHRNFI A+ MVT DQ+   +  NVFLCFLPMFH+
Sbjct: 196 ---QSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLCFLPMFHI 255

Query: 255 FGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQKVVKNYD 314
           FGLS++  +QL RGN +++M++F++   +  V ++++THL+ VPPVIIAL K      YD
Sbjct: 256 FGLSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKHGKAGKYD 315

Query: 315 LSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGV-ESTQSGAT 374
           LSSL+ I SGAAPLGKDVM+  +K  P + I+QGYGMTETCG+IS+E     ++ + G+T
Sbjct: 316 LSSLKFIGSGAAPLGKDVMEVVAKKFPDSEIVQGYGMTETCGIISLEYPEKGQAREFGST 375

Query: 375 GSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGD 434
           G+LVSGVEA+I+ I+T K LPP + GEICVRGPN+M+GYFNN +AT  TI  QGW+HTGD
Sbjct: 376 GTLVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTI-KQGWLHTGD 435

Query: 435 IGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAGEVPI 494
           +GYF+  G+LFVVDR+KELIK  GFQ+APAELE LLLSHP+I DA+VIP PD KAGEVPI
Sbjct: 436 LGYFDGGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDAKAGEVPI 495

Query: 495 AFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQVRA 544
           A+VVRSP+SS++E DV+ F+  QVA +KRL++VTF  SVPKSASGK+LRR++IAQVR+
Sbjct: 496 AYVVRSPDSSLTEVDVQKFIEKQVAYYKRLKRVTFVGSVPKSASGKILRRQLIAQVRS 549

BLAST of CsGy3G031120.2 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 5.5e-122
Identity = 231/536 (43.10%), Postives = 352/536 (65.67%), Query Frame = 0

Query: 13  SQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLTFRQLQIQVSKLA 72
           + ++ S+R P+  P + ++ + +F+  +S ++   +A +DA +G  LTF QL   V  +A
Sbjct: 19  NSIFYSKREPVQLPQNHSVDVTTFI--SSRAHHGKIAFIDAATGRHLTFPQLWRAVDSVA 78

Query: 73  HVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSAELSKQVANCKPK 132
                +GI+KGDV+L+ +PNSI+F V   A++++GAI TT NP  T  E++KQ+ + KP 
Sbjct: 79  TCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAKQITDSKPV 138

Query: 133 LVITVPELWDVIGKLNLPSIILGSKISSKFSRS-NIWAYSDLIKKAGDVSNLPVSEVGQN 192
           L  T+P+L   I   NLP +I+  ++ S   ++ NI +    + +     N     V Q 
Sbjct: 139 LAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMRKEPSPNRIGYRVNQE 198

Query: 193 DVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCFLPMFHVFGLSI 252
           D A LLYSSGTTG SKGV+ +H+N I A +     +    D  + F+C +PMFH++GL+ 
Sbjct: 199 DTATLLYSSGTTGASKGVVSSHKNLI-AMVQTILSRFGTEDGEHTFICTVPMFHIYGLAA 258

Query: 253 VVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTK--QKVVKNYDLSS 312
                L  G+T+V ++KFE+ + L  + KY+ T+L +VPP+++AL K    +   YDLSS
Sbjct: 259 FAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANHIRAKYDLSS 318

Query: 313 LRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVESTQSGATGSLV 372
           L+ +LSG APL K+V++   +  P   I+QGYG+TE+ G+ +  +   ES + G  G L 
Sbjct: 319 LQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESRRYGTAGMLS 378

Query: 373 SGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYF 432
             +EA+I++ ET + L    TGE+ +RGP +MKGYF+N++ATS TID +GW+ TGD+ Y 
Sbjct: 379 PSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEGWLRTGDLCYI 438

Query: 433 NEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAGEVPIAFVV 492
           +E+G +FVVDR+KELIK  G+QVAPAELEALLLSHP+I+DA VIP+PD +AG+ P+A+VV
Sbjct: 439 DEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKEAGQFPMAYVV 498

Query: 493 RSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQVRAKM 546
           R   S++SE  V  F+A  VAP+KR+RKV F +S+PK+ SGK+LR+++I    +K+
Sbjct: 499 RKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLIKLATSKL 551

BLAST of CsGy3G031120.2 vs. NCBI nr
Match: XP_011651966.2 (4-coumarate--CoA ligase-like 7 [Cucumis sativus] >KAE8651043.1 hypothetical protein Csa_001162 [Cucumis sativus])

HSP 1 Score: 1059 bits (2739), Expect = 0.0
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60
           MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT
Sbjct: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120
           FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA
Sbjct: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120

Query: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180
           ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV
Sbjct: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300
           LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK
Sbjct: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360
           VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360

Query: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420
           QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480

Query: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540
           GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ
Sbjct: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540

Query: 541 VRAKM 545
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CsGy3G031120.2 vs. NCBI nr
Match: XP_008442710.1 (PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo] >KAA0043992.1 4-coumarate--CoA ligase-like 7 [Cucumis melo var. makuwa] >TYK25148.1 4-coumarate--CoA ligase-like 7 [Cucumis melo var. makuwa])

HSP 1 Score: 1015 bits (2625), Expect = 0.0
Identity = 520/545 (95.41%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60
           M+ITMNKSFNPQS++Y+S RPPIHFPTDP ISIVSFLFRNSSSYPNALAL DADSGESLT
Sbjct: 1   MEITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120
           FRQLQIQVSKLA VF QLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYT A
Sbjct: 61  FRQLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180
           ELSKQVANC PKLVITVPELWDVIGKLNLPS+ILGSKISSKFSRSNIW+YSDLIKKAGDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFIT SLMVTQDQELLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300
           LPMFHVFGLS++V SQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK
Sbjct: 241 LPMFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360
           VVKNYDLSSLRQILSGAAPLGKDVM+ECSKIIPQARIIQGYGMTETCGVISVEN+ VEST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARIIQGYGMTETCGVISVENMVVEST 360

Query: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420
            SGATGSL SG+EAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGATGSLGSGIEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480

Query: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540
           GEVPIAFVVRSPNSSISEEDVK F+AGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ
Sbjct: 481 GEVPIAFVVRSPNSSISEEDVKKFIAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540

Query: 541 VRAKM 545
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CsGy3G031120.2 vs. NCBI nr
Match: XP_038905825.1 (4-coumarate--CoA ligase-like 7 isoform X1 [Benincasa hispida])

HSP 1 Score: 990 bits (2559), Expect = 0.0
Identity = 503/545 (92.29%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60
           M ITMNKSFNPQSQ+YTSRRPPIHFPTDPTISI SFLFRNSS+YPNALAL DADSGESLT
Sbjct: 1   MAITMNKSFNPQSQIYTSRRPPIHFPTDPTISIASFLFRNSSNYPNALALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120
           FRQ +IQVSKLAHV+IQLGI+KGDVVLIFAPNSIHF VCFFAIVAIGAI TTCNPAYT A
Sbjct: 61  FRQFKIQVSKLAHVYIQLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAITTTCNPAYTFA 120

Query: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180
           ELSKQVANC PKLVIT+PELWDV+GKLNLPSIILGSKISSKFSR NIW YSDLIKKAGDV
Sbjct: 121 ELSKQVANCNPKLVITIPELWDVVGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFI  SLMVTQDQ+LLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFIATSLMVTQDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300
           LPMFHVFGLSIVV +QLQRGNTVVSMAKFELEKALGLVMKY+ITHLYVVPPVIIAL KQ 
Sbjct: 241 LPMFHVFGLSIVVYAQLQRGNTVVSMAKFELEKALGLVMKYRITHLYVVPPVIIALAKQS 300

Query: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360
           VVKNYDLSSLRQILSGAAPLGKDVM+ECSKIIP ARIIQGYGMTETCGVISVENVG+EST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPHARIIQGYGMTETCGVISVENVGIEST 360

Query: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420
            SGATGSLVSGVEA+ILS ETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGATGSLVSGVEAEILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIP+PDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480

Query: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540
           GEVPIAFVV SP+S+I EEDVK F+A QVAPFKRLR+VTFT SVPKSASGKLLRREV+AQ
Sbjct: 481 GEVPIAFVVCSPDSTIKEEDVKKFIARQVAPFKRLRRVTFTRSVPKSASGKLLRREVVAQ 540

Query: 541 VRAKM 545
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CsGy3G031120.2 vs. NCBI nr
Match: XP_022982901.1 (4-coumarate--CoA ligase-like 7 [Cucurbita maxima])

HSP 1 Score: 950 bits (2456), Expect = 0.0
Identity = 478/545 (87.71%), Postives = 515/545 (94.50%), Query Frame = 0

Query: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60
           M I M KSFNPQSQ+YTS RPPIHFPTDPTISI+SFLFRNSSS+ ++LAL DADSGESLT
Sbjct: 1   MAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISILSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120
           FRQLQIQVSKLAH FI LGI+KGDVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYT A
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180
           E+SKQVA+C PKLVIT+PELWDVIGKLNLPSIILGSKISSK SR NIW YSDLIK +GDV
Sbjct: 121 EISKQVASCNPKLVITIPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240
           S+LPVSEVGQ+DVAALLYSSGTTGI+KGVILTHRNFIT SLMVT DQ+LLGDPRNVFLCF
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGINKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300
           LPMFHVFGLS+++ SQLQRGNTVVSMAKFELEKALG+VMKY+ITHLY+VPPVIIA+ KQ 
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360
           VVK YDLSSL+QILSGAAPLGKDVM+ECSK++PQ +I QGYGMTETCGVIS+EN+GVES 
Sbjct: 301 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420
            SG+TG LVSG+EAQILS ETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQI DAIVIP+PDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKA 480

Query: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540
           GEVPIAFVVRSP+SSI+EEDVK F+AGQVAPFKRL+ VTFTSS+PKSASGKLLRRE+IAQ
Sbjct: 481 GEVPIAFVVRSPSSSINEEDVKSFIAGQVAPFKRLKTVTFTSSIPKSASGKLLRRELIAQ 540

Query: 541 VRAKM 545
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CsGy3G031120.2 vs. NCBI nr
Match: XP_023526195.1 (4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 479/543 (88.21%), Postives = 513/543 (94.48%), Query Frame = 0

Query: 3   ITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLTFR 62
           I M KSFNPQSQ+YTS RPPIHFPTDPTISIVSFLFRNSSS+ + LAL DADSGESLTFR
Sbjct: 5   IAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFR 64

Query: 63  QLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSAEL 122
           QLQIQVS+LAH FI LGI+KGDVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYT AEL
Sbjct: 65  QLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAEL 124

Query: 123 SKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDVSN 182
           SKQVANC PKLVITVPELWDVIGKLNLPS+ILGSKISS+ SR NIW YSDLIK +GDVS+
Sbjct: 125 SKQVANCNPKLVITVPELWDVIGKLNLPSVILGSKISSESSRPNIWVYSDLIKNSGDVSD 184

Query: 183 LPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCFLP 242
           LPVSEVGQ+DVAALLYSSGTTGISKGVILTHRNFIT SLMVT DQ+LLGDPRNVFLCFLP
Sbjct: 185 LPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLCFLP 244

Query: 243 MFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQKVV 302
           MFHVFGLS+++ SQLQRGNTVVSMAKFELEKALG+VMKY+ITHLY+VPPVIIA+ KQ VV
Sbjct: 245 MFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSVV 304

Query: 303 KNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVESTQS 362
           K YDLSSL+QILSGAAPLGKDVM+ECSK++PQ +I QGYGMTETCGVIS+EN+GVES  S
Sbjct: 305 KRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRLS 364

Query: 363 GATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH 422
           G+TG LVSG+EAQILS ETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH
Sbjct: 365 GSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH 424

Query: 423 TGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAGE 482
           TGDIGYFNEEGELFVVDRIKELIKCYG+QVAPAELEALLLSHPQI DAIVIP+PDDKAGE
Sbjct: 425 TGDIGYFNEEGELFVVDRIKELIKCYGYQVAPAELEALLLSHPQIIDAIVIPYPDDKAGE 484

Query: 483 VPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQVR 542
           VPIAFVVRSPN+SI+EEDVK F+AGQVAPFKRL+ VTFTSSVPKSASGKLLRRE+IAQVR
Sbjct: 485 VPIAFVVRSPNNSINEEDVKSFIAGQVAPFKRLKIVTFTSSVPKSASGKLLRRELIAQVR 544

Query: 543 AKM 545
           AKM
Sbjct: 545 AKM 547

BLAST of CsGy3G031120.2 vs. ExPASy TrEMBL
Match: A0A0A0LDK0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740270 PE=4 SV=1)

HSP 1 Score: 1056 bits (2732), Expect = 0.0
Identity = 543/545 (99.63%), Postives = 544/545 (99.82%), Query Frame = 0

Query: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60
           MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT
Sbjct: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120
           FRQLQIQVSKLAHVFIQLGIQKGDVVLIF+PNSIHFLVCFFAIVAIGAIATTCNPAYTSA
Sbjct: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFSPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120

Query: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180
           ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIW YSDLIKKAGDV
Sbjct: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWGYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300
           LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK
Sbjct: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360
           VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360

Query: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420
           QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480

Query: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540
           GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ
Sbjct: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540

Query: 541 VRAKM 545
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CsGy3G031120.2 vs. ExPASy TrEMBL
Match: A0A5A7TKZ4 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002730 PE=4 SV=1)

HSP 1 Score: 1015 bits (2625), Expect = 0.0
Identity = 520/545 (95.41%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60
           M+ITMNKSFNPQS++Y+S RPPIHFPTDP ISIVSFLFRNSSSYPNALAL DADSGESLT
Sbjct: 1   MEITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120
           FRQLQIQVSKLA VF QLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYT A
Sbjct: 61  FRQLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180
           ELSKQVANC PKLVITVPELWDVIGKLNLPS+ILGSKISSKFSRSNIW+YSDLIKKAGDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFIT SLMVTQDQELLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300
           LPMFHVFGLS++V SQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK
Sbjct: 241 LPMFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360
           VVKNYDLSSLRQILSGAAPLGKDVM+ECSKIIPQARIIQGYGMTETCGVISVEN+ VEST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARIIQGYGMTETCGVISVENMVVEST 360

Query: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420
            SGATGSL SG+EAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGATGSLGSGIEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480

Query: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540
           GEVPIAFVVRSPNSSISEEDVK F+AGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ
Sbjct: 481 GEVPIAFVVRSPNSSISEEDVKKFIAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540

Query: 541 VRAKM 545
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CsGy3G031120.2 vs. ExPASy TrEMBL
Match: A0A1S3B5U8 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103486502 PE=4 SV=1)

HSP 1 Score: 1015 bits (2625), Expect = 0.0
Identity = 520/545 (95.41%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60
           M+ITMNKSFNPQS++Y+S RPPIHFPTDP ISIVSFLFRNSSSYPNALAL DADSGESLT
Sbjct: 1   MEITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120
           FRQLQIQVSKLA VF QLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYT A
Sbjct: 61  FRQLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180
           ELSKQVANC PKLVITVPELWDVIGKLNLPS+ILGSKISSKFSRSNIW+YSDLIKKAGDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFIT SLMVTQDQELLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300
           LPMFHVFGLS++V SQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK
Sbjct: 241 LPMFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360
           VVKNYDLSSLRQILSGAAPLGKDVM+ECSKIIPQARIIQGYGMTETCGVISVEN+ VEST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARIIQGYGMTETCGVISVENMVVEST 360

Query: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420
            SGATGSL SG+EAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGATGSLGSGIEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480

Query: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540
           GEVPIAFVVRSPNSSISEEDVK F+AGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ
Sbjct: 481 GEVPIAFVVRSPNSSISEEDVKKFIAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540

Query: 541 VRAKM 545
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CsGy3G031120.2 vs. ExPASy TrEMBL
Match: A0A6J1J0P0 (4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111481607 PE=4 SV=1)

HSP 1 Score: 950 bits (2456), Expect = 0.0
Identity = 478/545 (87.71%), Postives = 515/545 (94.50%), Query Frame = 0

Query: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60
           M I M KSFNPQSQ+YTS RPPIHFPTDPTISI+SFLFRNSSS+ ++LAL DADSGESLT
Sbjct: 1   MAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISILSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120
           FRQLQIQVSKLAH FI LGI+KGDVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYT A
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180
           E+SKQVA+C PKLVIT+PELWDVIGKLNLPSIILGSKISSK SR NIW YSDLIK +GDV
Sbjct: 121 EISKQVASCNPKLVITIPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240
           S+LPVSEVGQ+DVAALLYSSGTTGI+KGVILTHRNFIT SLMVT DQ+LLGDPRNVFLCF
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGINKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300
           LPMFHVFGLS+++ SQLQRGNTVVSMAKFELEKALG+VMKY+ITHLY+VPPVIIA+ KQ 
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360
           VVK YDLSSL+QILSGAAPLGKDVM+ECSK++PQ +I QGYGMTETCGVIS+EN+GVES 
Sbjct: 301 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420
            SG+TG LVSG+EAQILS ETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQI DAIVIP+PDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKA 480

Query: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540
           GEVPIAFVVRSP+SSI+EEDVK F+AGQVAPFKRL+ VTFTSS+PKSASGKLLRRE+IAQ
Sbjct: 481 GEVPIAFVVRSPSSSINEEDVKSFIAGQVAPFKRLKTVTFTSSIPKSASGKLLRRELIAQ 540

Query: 541 VRAKM 545
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CsGy3G031120.2 vs. ExPASy TrEMBL
Match: A0A6J1F2M7 (4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111441628 PE=4 SV=1)

HSP 1 Score: 947 bits (2448), Expect = 0.0
Identity = 478/545 (87.71%), Postives = 512/545 (93.94%), Query Frame = 0

Query: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60
           M I M KSF+PQSQ+YTS RPPIHFPTDPTISIVSF+FRNSSS+ ++LAL DADSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFVFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120
           FRQLQIQVSKLAH FI LGI+KGDVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYT  
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180
           ELSKQVANC PKLVITVPELWDVIGKLNLPSIILGS+ISSK SR NIW YSDLIK +GDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSEISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240
           S+ PVSEVGQ+DVAALLYSSGTTGISKGVILTHRNFI  SLMVT DQ+LLGDPRNVFLCF
Sbjct: 181 SDFPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300
           LPMFHVFGLS+++ SQLQRGNTVVSMAKFELEKALG+VMKY+ITHLY+VPPVIIA+ KQ 
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360
           VVK YDLSSL+QILSGAAPLGKDVM+ECSK++PQ +I QGYGMTETCGVIS+EN+GVES 
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420
            SG+TG LVSG+EAQILS ETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGSTGLLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQ+ DAIVIP+PDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQVIDAIVIPYPDDKA 480

Query: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540
           GEVPIAFVVRSPNSSI+EEDVK F+AGQVAPFKRL+ VTFTSSVPKSASGKLLRRE+IAQ
Sbjct: 481 GEVPIAFVVRSPNSSINEEDVKSFIAGQVAPFKRLKIVTFTSSVPKSASGKLLRRELIAQ 540

Query: 541 VRAKM 545
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CsGy3G031120.2 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 657.9 bits (1696), Expect = 7.1e-189
Identity = 331/544 (60.85%), Postives = 424/544 (77.94%), Query Frame = 0

Query: 5   MNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLTFRQL 64
           M KS   +  +Y S RP +  P DP  S+VSFLFRNSSSYP+ LA+ D+D+G+SLTF QL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 65  QIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSAELSK 124
           +  V++LAH F +LGI+K DVVLIFAPNS  F +CF A+ AIG + TT NP YT  E+SK
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 125 QVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKF---SRSNIWAYSDLIKKAGDVS 184
           Q+ +  PK++I+V +L+D I   +LP ++LGSK + +    S S I ++ ++++ +  VS
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVS 180

Query: 185 NLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCFL 244
             P  E+ Q+D AALLYSSGTTG SKGV LTH NFI ASLMVT DQ+L+G+   VFLCFL
Sbjct: 181 EYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFL 240

Query: 245 PMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQKV 304
           PMFHVFGL+++  SQLQRGN +VSMA+FELE  L  + K+++THL+VVPPV +AL+KQ +
Sbjct: 241 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 300

Query: 305 VKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVESTQ 364
           VK +DLSSL+ I SGAAPLGKD+M+EC + IP   ++QGYGMTETCG++SVE+  +    
Sbjct: 301 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRN 360

Query: 365 SGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWV 424
           SG+ G L  GVEAQI+S+ET K  PP + GEI VRGPNMMKGY NN +AT +TID + WV
Sbjct: 361 SGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWV 420

Query: 425 HTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAG 484
           HTGD+GYFNE+G L+VVDRIKELIK  GFQVAPAELE LL+SHP I DA+VIP PD++AG
Sbjct: 421 HTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAG 480

Query: 485 EVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQV 544
           EVPIAFVVRSPNSSI+E+D++ F+A QVAP+KRLR+V+F S VPKSA+GK+LRRE++ QV
Sbjct: 481 EVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQV 540

Query: 545 RAKM 546
           R+KM
Sbjct: 541 RSKM 544

BLAST of CsGy3G031120.2 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 420.6 bits (1080), Expect = 1.9e-117
Identity = 223/540 (41.30%), Postives = 349/540 (64.63%), Query Frame = 0

Query: 3   ITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLTFR 62
           +     F   +  + S+R PI  P +P++ + +F+  +S ++   +A +DA +G++LTF 
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFI--SSQAHRGRIAFIDASTGQNLTFT 63

Query: 63  QLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSAEL 122
           +L   V  +A    ++GI+KG VVL+ +PNSI F V   +++++GAI TT NP  TS E+
Sbjct: 64  ELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEI 123

Query: 123 SKQVANCKPKLVITVPELWDVIGKL--NLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 182
           +KQ+ +  P L  T  +L   I      LP +++  +     S  ++    +++KK    
Sbjct: 124 AKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVD--SVGDVRRLVEMMKKEPS- 183

Query: 183 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 242
            N     V Q+D A LLYSSGTTG+SKGVI +HRN I A +    ++    D    F+C 
Sbjct: 184 GNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLI-AMVQTIVNRFGSDDGEQRFICT 243

Query: 243 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTK-- 302
           +PMFH++GL+      L  G+T++ ++KFE+ + +  + KY+ T L +VPP+++A+    
Sbjct: 244 VPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 303

Query: 303 QKVVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVE 362
            ++   YDLSS+  +L G APL K+V +  ++  P  +I+QGYG+TE+ G+ +  +   E
Sbjct: 304 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEE 363

Query: 363 STQSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQ 422
           S + G  G L + +E +I+   T + L P +TGE+ ++GP++MKGYF+N++ATS T+D +
Sbjct: 364 SRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSE 423

Query: 423 GWVHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDD 482
           GW+ TGD+ Y +E+G +FVVDR+KELIK  G+QVAPAELEALLL+HP+ITDA VIP PD 
Sbjct: 424 GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDK 483

Query: 483 KAGEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVI 539
           + G+ P+A+VVR   SS+SE+ +  FVA QVAP+KR+RKV F SS+PK+ SGK+LR+++I
Sbjct: 484 EVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLI 537

BLAST of CsGy3G031120.2 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 406.4 bits (1043), Expect = 3.7e-113
Identity = 226/537 (42.09%), Postives = 342/537 (63.69%), Query Frame = 0

Query: 11  PQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLTFRQLQIQVSK 70
           P  +++ S+ P I  P    + + ++ F   SS  +   L+   +G+S T+ +  +   +
Sbjct: 30  PTPRIFRSKLPDIDIPNH--LPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRR 89

Query: 71  LAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSAELSKQVANCK 130
           +A    +LGI+KGDV++I   NS  F+  F     IGA++TT NP YTS EL KQ+ +  
Sbjct: 90  VASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSG 149

Query: 131 PKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDVSNLPVSEVGQ 190
            KL+IT  +  D +  L     ++    + + +  N   +S LI            ++G 
Sbjct: 150 AKLIITHSQYVDKLKNLGENLTLI---TTDEPTPENCLPFSTLITDDETNPFQETVDIGG 209

Query: 191 NDVAALLYSSGTTGISKGVILTHRNFIT--ASLMVTQDQELLGDPRNVFLCFLPMFHVFG 250
           +D AAL +SSGTTG+ KGV+LTH++ IT  A  +   +  L     +V LC LP+FH++ 
Sbjct: 210 DDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYS 269

Query: 251 LSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQKVVKNYDLS 310
           L+ V+ + L+ G TV+ M KFE+   L L+ ++++T   +VPP++IAL K   V +YDLS
Sbjct: 270 LNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLS 329

Query: 311 SLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVES----TQSGA 370
           S+R +LSGAAPLGK++ D   + +PQA + QGYGMTE   V+S+ ++G       T+SG+
Sbjct: 330 SVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSM-SLGFAKEPIPTKSGS 389

Query: 371 TGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTG 430
            G++V   E +++ +ET+  L   + GEIC+RG  +MK Y N+ +ATS TID++GW+HTG
Sbjct: 390 CGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTG 449

Query: 431 DIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAGEVP 490
           DIGY +E+ E+F+VDR+KE+IK  GFQV PAELE+LL++H  I DA V+P  D+ AGEVP
Sbjct: 450 DIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVP 509

Query: 491 IAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQV 542
           +AFVVRS  + I+EEDVK +VA QV  +KRL KV F +S+PKS SGK+LR+++ A++
Sbjct: 510 VAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CsGy3G031120.2 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 7.0e-112
Identity = 217/534 (40.64%), Postives = 340/534 (63.67%), Query Frame = 0

Query: 9   FNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLTFRQLQIQV 68
           F+ ++ +YTS+ P +H P DP +  VS LF  S  +    AL+D+ +G S++  +LQI V
Sbjct: 25  FSSKTGIYTSKFPSLHLPVDPNLDAVSALF--SHKHHGDTALIDSLTGFSISHTELQIMV 84

Query: 69  -SKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSAELSKQVA 128
            S  A ++  LG+++GDVV +  PNS++F + F +++++GAI TT NP+ +  E+ KQV+
Sbjct: 85  QSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQVS 144

Query: 129 NCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDVSNLPVSE 188
            C   L  T  E  + +  L +  I +         R     +  ++K++     +P   
Sbjct: 145 ECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMKES--FGFVPKPL 204

Query: 189 VGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQ---DQELLGDPRNVFLCFLPMF 248
           + Q+DVAA++YSSGTTG SKGV+LTHRN I +  +  +    Q       NV+L  LP+ 
Sbjct: 205 IKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLC 264

Query: 249 HVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQ-KVVK 308
           H++GLS+ V   L  G+T+V M +F+    + ++ ++KITH  VVPP+++ALTK+ K V 
Sbjct: 265 HIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKGVC 324

Query: 309 NYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVESTQSG 368
                SL+Q+ SGAAPL +  +++  + +P   +IQGYGMTE+  V +      + ++  
Sbjct: 325 GEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYS 384

Query: 369 ATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHT 428
           + G L   ++A+++   +   LPPG  GE+ ++GP +MKGY NN KAT  +I +  W+ T
Sbjct: 385 SVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRT 444

Query: 429 GDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAGEV 488
           GDI YF+E+G LF+VDRIKE+IK  GFQ+APA+LEA+L+SHP I DA V   P+++ GE+
Sbjct: 445 GDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEI 504

Query: 489 PIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREV 538
           P+AFVVR   +++SEEDV  +VA QVAP++++RKV   +S+PKS +GK+LR+E+
Sbjct: 505 PVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILRKEL 554

BLAST of CsGy3G031120.2 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 396.7 bits (1018), Expect = 2.9e-110
Identity = 224/552 (40.58%), Postives = 340/552 (61.59%), Query Frame = 0

Query: 2   DITMNKSFNPQSQ-----VYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSG 61
           D+ +N   N Q Q     ++ SR P I+ P    + +  ++F N S +     L++  +G
Sbjct: 5   DVIVNDQ-NDQKQCSNDVIFRSRLPDIYIPNH--LPLHDYIFENISEFAAKPCLINGPTG 64

Query: 62  ESLTFRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPA 121
           E  T+  + +   KLA     LG+++ DVV+I  PNS   ++ F A   IGAI T+ NP 
Sbjct: 65  EVYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPF 124

Query: 122 YTSAELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIK- 181
           +T AE+SKQ      KL++T     D I  L    +++ +  S      N   +S+L + 
Sbjct: 125 FTPAEISKQAKASAAKLIVTQSRYVDKIKNLQNDGVLIVTTDSDAIP-ENCLRFSELTQS 184

Query: 182 KAGDVSNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFIT--ASLMVTQDQELLGDP 241
           +   V ++P  ++   DV AL +SSGTTG+ KGV+LTH+  +T  A  +  ++  L  + 
Sbjct: 185 EEPRVDSIP-EKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNR 244

Query: 242 RNVFLCFLPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVI 301
            +V LC LPMFH++ L+ ++   L+ G T++ M KFE+   L  + + K+T   VVPP++
Sbjct: 245 DDVILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIV 304

Query: 302 IALTKQKVVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVE 361
           +A+ K    + YDLSS+R + SGAAPLGK++ D  S   P A++ QGYGMTE  G +   
Sbjct: 305 LAIAKSPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTE-AGPVLAM 364

Query: 362 NVGVES----TQSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQK 421
           ++G        +SGA G++V   E +IL  +T   LP  + GEIC+RG  +MKGY N+  
Sbjct: 365 SLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPL 424

Query: 422 ATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITD 481
           AT+ TID  GW+HTGD+G+ +++ ELF+VDR+KELIK  GFQVAPAELE+LL+ HP+I D
Sbjct: 425 ATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEIND 484

Query: 482 AIVIPHPDDKAGEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSAS 541
             V+   ++ AGEVP+AFVVRS +S+ISE+++K FV+ QV  +KR+ KV FT S+PK+ S
Sbjct: 485 VAVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPS 544