CsGy3G007780.2 (mRNA) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G007780.2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	cytochrome b5-like
Location	Gy14Chr3: 6643353 .. 6645519 (-)
Sequence length	1111
RNA-Seq Expression	CsGy3G007780.2
Synteny	CsGy3G007780.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATGGGAAAATAAAGGAAGATTTGGGGGGGAGTTTGTAAAAGAGAGGGAAAACTAAGGACTGAAATGAAATAGTTTGTAACAACAAATTCATCTGAGTTGTCTTCAAGCGTAACCTGCCAAATAAGAGTCGCAAGAAATGGCTTGGCCGGTGGTGCTTCTAGTCAAATATAGAAGACAACATACCTTGCTCATTTGATTTTCTCCTTTCTTCATTTTCTTTCAAACTTTAATCTGTAAGAAACCCATCTTCTGTATTTTCAATCAGTTTTGAATTGGAGCTTCATTTTTGTTTGCACTTGATTGTTGCAGTAATGGCTTCGGATCCTAAGCTTTTCGTCTTCGATGAAGTGGCTAAACATAATCACCAGGCGGATTGTTGGCTTATAATCTCTGGAAAGGTCTGATATATTTTGTTTTTCTGGTATTTTGTGAAATTATATGTTTACAACTCAATCGTACTTCCCCATTGGAACAGTAGACTCATATGAGACCAGATTGAGGTATGGCAGATTAAGTTTCTTTGTTTCTTCTACCCATCTTCACAAACTGATAGACTGGGTATTCGGTTTAAATACTACTTTGACCGAATTTTTAACTTTGATTAAAGTGTCAGTTTCTCGAAAGTACAGGAACTAAGTAAAATACATCTTTACTGCATGGCATTTGATGCTCAAGAAACTTGTAGAGGTTGAGATACAGGACACTGATCTTTGTATAATGGAAACTAAATGCACAGAAAAGCTGTTGTTTAGCACTAGAGGAAAAATTTGGGCTTCCAAATTATTGAGTTTAGCTACTTAGTATGTGTAGGATTCGAAACTCTAACTTCTATTACCCACAGAGTCTATCTCTGAAGGTCTAGGTGAGCAGAGACTAAGAGTAATACTTGAAGAGTACATCTCAGAACTTCGACTAACATATTCTATGTAGAATATTGAAATGCTTTTATGGATTGTCGGGATGCCTGTTTAATGCAACAAGTCTCTCGGTCTCCCTTTTATACGTCTAAGTTTCAAGCATGGAACTTGGGAATGGAAGATACATTGTAAGCCATGACAACATATCACTAGAGAGTGGAATGAAATTACAGCTTCTGAAATTGTTTCTGCTGTTGTTATATCAGTACTCGTCTACCAGATATGCATATAATCAGACCTTTCTAACTTGACTGTCCAAATTTATATAGATATTTTCCTTATTTATTCGTATTGTCATTCTGTTTCACTGCTTTGTCCCTGTGCATTTCCCATGATAGGTTTATGATGTCACTCCTTTTCTGGAAGATCATCCAGGAGGTGATGAAGTCTTATTATTAGCTACTGGTAAGTTTTTGGGTCAACATGTTTTCTCCAAATTGACTACTTGTCACTGTATTTCAAGACATCCAACAAAGATGATTGCATCAAGTTATGAATGATTGGAAAAACAATTTTAAATCTGTGCAGAGAAAGATGCAACTGAAGACTTTGAGACTGTGAGTCACAGTTTAGATGCAACAGAGGAAATGGAAAAATATTATATTGGCAATATTGACATGTCTACGATTCCAAAGCCGGCTGATCATCGACCACCTGCATCCAAATCAGAATCGGCAGCAACCGAAGCAGCAACAGAAAAAACATCGTCGGCTCAATCTTCAGGATCTTTGATAAAAGTGTTGCAAGTTTTGATTCCTTTGTTGATTATAGGTGTTGCATTTTATCTGCAATACTATGGCAAAAAGCAGTAATCGACATTCTCAAATCATGGATGCCTTGAGTTTTGCAATAAGCCAGACATCTCCTCATCACATTCTGTTGAACACACTCTTATCTAGGTTTCTCTGCCCTTTTGACTCTTGCTAATTTGTGAGTAGAGAATTTTCATTTGTTTCTTGTTTCATAATCCTGCTTTACTCTGTCTTTTGTTTTACAATATTCAAGTTTCTGTGCTGATTTAACACACCAGATATATCAGGTTGAAGGTAGATGAAATAATGGATTGTCTAAATTTGGTCCTTTCCCACTTGCCAACCCTTCTTCATGGAATTATAGTAGGTGTGTTTTAATTTTAATTACACAAATGAGAATGACAAGCATCGTTACTGATTGGGAAAGGATTAGAGATATCGAAAATTGATCTGGTTGAAATGATATTGTTTTCTTTTCTAGTTCAATCATTGCAAG

mRNA sequence

GGATGGGAAAATAAAGGAAGATTTGGGGGGGAGTTTGTAAAAGAGAGGGAAAACTAAGGACTGAAATGAAATAGTTTGTAACAACAAATTCATCTGAGTTGTCTTCAAGCGTAACCTGCCAAATAAGAGTCGCAAGAAATGGCTTGGCCGGTGGTGCTTCTAGTCAAATATAGAAGACAACATACCTTGCTCATTTGATTTTCTCCTTTCTTCATTTTCTTTCAAACTTTAATCTTAATGGCTTCGGATCCTAAGCTTTTCGTCTTCGATGAAGTGGCTAAACATAATCACCAGGCGGATTGTTGGCTTATAATCTCTGGAAAGGTTTATGATGTCACTCCTTTTCTGGAAGATCATCCAGGAGGTGATGAAGTCTTATTATTAGCTACTGAGAAAGATGCAACTGAAGACTTTGAGACTGTGAGTCACAGTTTAGATGCAACAGAGGAAATGGAAAAATATTATATTGGCAATATTGACATGTCTACGATTCCAAAGCCGGCTGATCATCGACCACCTGCATCCAAATCAGAATCGGCAGCAACCGAAGCAGCAACAGAAAAAACATCGTCGGCTCAATCTTCAGGATCTTTGATAAAAGTGTTGCAAGTTTTGATTCCTTTGTTGATTATAGGTGTTGCATTTTATCTGCAATACTATGGCAAAAAGCAGTAATCGACATTCTCAAATCATGGATGCCTTGAGTTTTGCAATAAGCCAGACATCTCCTCATCACATTCTGTTGAACACACTCTTATCTAGGTTTCTCTGCCCTTTTGACTCTTGCTAATTTGTGAGTAGAGAATTTTCATTTGTTTCTTGTTTCATAATCCTGCTTTACTCTGTCTTTTGTTTTACAATATTCAAGTTTCTGTGCTGATTTAACACACCAGATATATCAGGTTGAAGGTAGATGAAATAATGGATTGTCTAAATTTGGTCCTTTCCCACTTGCCAACCCTTCTTCATGGAATTATAGTAGGTGTGTTTTAATTTTAATTACACAAATGAGAATGACAAGCATCGTTACTGATTGGGAAAGGATTAGAGATATCGAAAATTGATCTGGTTGAAATGATATTGTTTTCTTTTCTAGTTCAATCATTGCAAG

Coding sequence (CDS)

ATGGCTTCGGATCCTAAGCTTTTCGTCTTCGATGAAGTGGCTAAACATAATCACCAGGCGGATTGTTGGCTTATAATCTCTGGAAAGGTTTATGATGTCACTCCTTTTCTGGAAGATCATCCAGGAGGTGATGAAGTCTTATTATTAGCTACTGAGAAAGATGCAACTGAAGACTTTGAGACTGTGAGTCACAGTTTAGATGCAACAGAGGAAATGGAAAAATATTATATTGGCAATATTGACATGTCTACGATTCCAAAGCCGGCTGATCATCGACCACCTGCATCCAAATCAGAATCGGCAGCAACCGAAGCAGCAACAGAAAAAACATCGTCGGCTCAATCTTCAGGATCTTTGATAAAAGTGTTGCAAGTTTTGATTCCTTTGTTGATTATAGGTGTTGCATTTTATCTGCAATACTATGGCAAAAAGCAGTAA

Protein sequence

MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFETVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLIKVLQVLIPLLIIGVAFYLQYYGKKQ*

Homology

[Close]

BLAST of CsGy3G007780.2 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 7.6e-34
Identity = 75/145 (51.72%), Postives = 101/145 (69.66%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MAS+ K+  F+EV++HN   DCWLIISGKVYDVTPF++DHPGGDEVLL +T KDAT DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A + MEKYYIG ID ST+        PA+++  A  + A  +  + +    +I
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTV--------PATRTYVAPVQPAYNQDKTPE---FMI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           K+LQ L+P+LI+G+A  ++ Y KK+
Sbjct: 121 KILQFLVPILILGLALVVRQYTKKE 134

[Close]

BLAST of CsGy3G007780.2 vs. ExPASy Swiss-Prot
Match: Q9ZWT2 (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 4.9e-33
Identity = 71/143 (49.65%), Postives = 100/143 (69.93%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M  D K+F   EV++H+   DCW++I GKVYDVT FL+DHPGGDEV+L +T KDAT+DFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A   +++YY+G+ID +T+P  A   PP S +++ AT+         +SS  +I
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTS-TKAVATQ--------DKSSDFVI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGK 144
           K+LQ L+PLLI+G+AF ++YY K
Sbjct: 121 KLLQFLVPLLILGLAFGIRYYTK 134

[Close]

BLAST of CsGy3G007780.2 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 4.9e-33
Identity = 73/144 (50.69%), Postives = 101/144 (70.14%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M+SD K+  F+EV+KHN   DCWLIISGKVYDVTPF++DHPGGDEVLL +T KDAT DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A + M+KY+IG ID S++        PA+++  A  + A  +  + +    +I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSV--------PATRTYVAPQQPAYNQDKTPE---FII 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKK 145
           K+LQ L+P+LI+G+A  +++Y KK
Sbjct: 121 KILQFLVPILILGLALVVRHYTKK 133

[Close]

BLAST of CsGy3G007780.2 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 6.4e-33
Identity = 71/144 (49.31%), Postives = 98/144 (68.06%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M  + K+F   EV++HN+  DCWL+ISGKVYDVT FL+DHPGGDEVLL AT KDAT+DFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A   +++YY+G+ID +TIP    + PP     +       +KTS       ++
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYN------QDKTSE-----FVV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKK 145
           K+LQ L+PL+I+GVAF +++Y K+
Sbjct: 121 KLLQFLVPLIILGVAFGIRFYTKQ 133

[Close]

BLAST of CsGy3G007780.2 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.6e-31
Identity = 71/144 (49.31%), Postives = 93/144 (64.58%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M    K+F   EV+ HN+  DCWLIISGKVY+VT FLEDHPGG EVLL AT KDAT+DFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            + HS  A   +++YY+G+ID STIP    + PP  K      +  TE          ++
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPP--KQPHYNQDKTTE---------FIV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKK 145
           K+LQ L+PL+I+GVAF + +Y K+
Sbjct: 121 KLLQFLVPLIILGVAFGVHFYTKQ 133

[Close]

BLAST of CsGy3G007780.2 vs. NCBI nr
Match: XP_004133794.1 (cytochrome b5 [Cucumis sativus] >KGN56392.1 hypothetical protein Csa_010413 [Cucumis sativus])

HSP 1 Score: 282 bits (721), Expect = 1.36e-95
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145
           KVLQVLIPLLIIGVAFYLQYYGKKQ
Sbjct: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of CsGy3G007780.2 vs. NCBI nr
Match: XP_008437843.1 (PREDICTED: cytochrome b5-like [Cucumis melo])

HSP 1 Score: 247 bits (630), Expect = 9.88e-82
Identity = 129/145 (88.97%), Postives = 133/145 (91.72%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQ DCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TV HSL ATEEMEKYYIGNIDMST+PK  D+RPPASKS S  T+AA E TSS QSSGSLI
Sbjct: 61  TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKSASTETKAAAETTSSNQSSGSLI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145
           KVLQ+LIPLLII VAFYLQYYGKKQ
Sbjct: 121 KVLQLLIPLLIIVVAFYLQYYGKKQ 144

[Close]

BLAST of CsGy3G007780.2 vs. NCBI nr
Match: TYK20812.1 (cytochrome b5-like [Cucumis melo var. makuwa])

HSP 1 Score: 247 bits (630), Expect = 5.86e-80
Identity = 129/145 (88.97%), Postives = 133/145 (91.72%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQ DCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 124 MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 183

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TV HSL ATEEMEKYYIGNIDMST+PK  D+RPPASKS S  T+AA E TSS QSSGSLI
Sbjct: 184 TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKSASTETKAAAETTSSNQSSGSLI 243

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145
           KVLQ+LIPLLII VAFYLQYYGKKQ
Sbjct: 244 KVLQLLIPLLIIVVAFYLQYYGKKQ 267

[Close]

BLAST of CsGy3G007780.2 vs. NCBI nr
Match: KAA0048860.1 (cytochrome b5-like [Cucumis melo var. makuwa])

HSP 1 Score: 247 bits (630), Expect = 5.86e-80
Identity = 129/145 (88.97%), Postives = 133/145 (91.72%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQ DCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 124 MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 183

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TV HSL ATEEMEKYYIGNIDMST+PK  D+RPPASKS S  T+AA E TSS QSSGSLI
Sbjct: 184 TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKSASTETKAAAETTSSNQSSGSLI 243

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145
           KVLQ+LIPLLII VAFYLQYYGKKQ
Sbjct: 244 KVLQLLIPLLIIVVAFYLQYYGKKQ 267

[Close]

BLAST of CsGy3G007780.2 vs. NCBI nr
Match: XP_038901262.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 240 bits (613), Expect = 3.86e-79
Identity = 123/145 (84.83%), Postives = 132/145 (91.03%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPK+FVFDEVAKHNHQ DCWLIISGKVYDVTPFLE+HPGGDEVLLLATEKDAT+DFE
Sbjct: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TV HSL ATEEMEKYY+GN+DMST+PKP D+RP AS+S  A+  A T  TSSAQSSGSLI
Sbjct: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSAKASATA-TITTSSAQSSGSLI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145
           KVLQ LIPLLIIGVAFYLQYYGKKQ
Sbjct: 121 KVLQYLIPLLIIGVAFYLQYYGKKQ 144

[Close]

BLAST of CsGy3G007780.2 vs. ExPASy TrEMBL
Match: A0A0A0L720 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119260 PE=3 SV=1)

HSP 1 Score: 282 bits (721), Expect = 6.59e-96
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145
           KVLQVLIPLLIIGVAFYLQYYGKKQ
Sbjct: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of CsGy3G007780.2 vs. ExPASy TrEMBL
Match: A0A1S3AV33 (cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103483149 PE=3 SV=1)

HSP 1 Score: 247 bits (630), Expect = 4.78e-82
Identity = 129/145 (88.97%), Postives = 133/145 (91.72%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQ DCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TV HSL ATEEMEKYYIGNIDMST+PK  D+RPPASKS S  T+AA E TSS QSSGSLI
Sbjct: 61  TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKSASTETKAAAETTSSNQSSGSLI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145
           KVLQ+LIPLLII VAFYLQYYGKKQ
Sbjct: 121 KVLQLLIPLLIIVVAFYLQYYGKKQ 144

[Close]

BLAST of CsGy3G007780.2 vs. ExPASy TrEMBL
Match: A0A5A7U0D0 (Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00740 PE=3 SV=1)

HSP 1 Score: 247 bits (630), Expect = 2.84e-80
Identity = 129/145 (88.97%), Postives = 133/145 (91.72%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQ DCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 124 MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 183

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TV HSL ATEEMEKYYIGNIDMST+PK  D+RPPASKS S  T+AA E TSS QSSGSLI
Sbjct: 184 TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKSASTETKAAAETTSSNQSSGSLI 243

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145
           KVLQ+LIPLLII VAFYLQYYGKKQ
Sbjct: 244 KVLQLLIPLLIIVVAFYLQYYGKKQ 267

[Close]

BLAST of CsGy3G007780.2 vs. ExPASy TrEMBL
Match: A0A5D3DB41 (Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00730 PE=3 SV=1)

HSP 1 Score: 247 bits (630), Expect = 2.84e-80
Identity = 129/145 (88.97%), Postives = 133/145 (91.72%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQ DCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 124 MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 183

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TV HSL ATEEMEKYYIGNIDMST+PK  D+RPPASKS S  T+AA E TSS QSSGSLI
Sbjct: 184 TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKSASTETKAAAETTSSNQSSGSLI 243

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145
           KVLQ+LIPLLII VAFYLQYYGKKQ
Sbjct: 244 KVLQLLIPLLIIVVAFYLQYYGKKQ 267

[Close]

BLAST of CsGy3G007780.2 vs. ExPASy TrEMBL
Match: A0A6J1D2G0 (cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111016385 PE=3 SV=1)

HSP 1 Score: 214 bits (545), Expect = 3.41e-69
Identity = 104/145 (71.72%), Postives = 124/145 (85.52%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M SDPK+F FDEVAKHNHQ DCWLIISGKVYDVTPFLE+HPGGDEVL+LA+EKDAT+DFE
Sbjct: 1   MGSDPKVFAFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLILASEKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HSL A ++ME+YY+GN+DMST+PKP D+RPPAS+S +          +SAQSSGS +
Sbjct: 61  NVGHSLSAIDQMEQYYVGNVDMSTVPKPIDYRPPASQSATT--------LASAQSSGSSL 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145
           K+LQ LIPLL+IGVAFY+Q+YGKKQ
Sbjct: 121 KLLQFLIPLLLIGVAFYMQFYGKKQ 137

[Close]

BLAST of CsGy3G007780.2 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 142.1 bits (357), Expect = 3.5e-34
Identity = 73/144 (50.69%), Postives = 101/144 (70.14%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M+SD K+  F+EV+KHN   DCWLIISGKVYDVTPF++DHPGGDEVLL +T KDAT DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A + M+KY+IG ID S++        PA+++  A  + A  +  + +    +I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSV--------PATRTYVAPQQPAYNQDKTPE---FII 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKK 145
           K+LQ L+P+LI+G+A  +++Y KK
Sbjct: 121 KILQFLVPILILGLALVVRHYTKK 133

[Close]

BLAST of CsGy3G007780.2 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 142.1 bits (357), Expect = 3.5e-34
Identity = 71/143 (49.65%), Postives = 100/143 (69.93%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M  D K+F   EV++H+   DCW++I GKVYDVT FL+DHPGGDEV+L +T KDAT+DFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A   +++YY+G+ID +T+P  A   PP S +++ AT+         +SS  +I
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTS-TKAVATQ--------DKSSDFVI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGK 144
           K+LQ L+PLLI+G+AF ++YY K
Sbjct: 121 KLLQFLVPLLILGLAFGIRYYTK 134

[Close]

BLAST of CsGy3G007780.2 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 136.7 bits (343), Expect = 1.5e-32
Identity = 70/143 (48.95%), Postives = 94/143 (65.73%), Query Frame = 0


Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M  + K+F   EV++HN   DCW++I+GKVY+VT FLEDHPGGD+VLL +T KDAT+DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A E ME+YY+G ID +TIPK   + PP     +       +KTS       +I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYN------QDKTSE-----FII 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGK 144
           K+LQ L+PL I+G+A  ++ Y K
Sbjct: 121 KLLQFLVPLAILGLAVGIRIYTK 132

[Close]

BLAST of CsGy3G007780.2 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 130.2 bits (326), Expect = 1.4e-30
Identity = 69/138 (50.00%), Postives = 91/138 (65.94%), Query Frame = 0


Query: 7   LFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFETVSHSL 66
           L  F +VAKH  + DCW++I GKVYD++ F+++HPGGD VLL  T KDA+ DFE V+HS 
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  DATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLIKVLQVL 126
           DA E M+KY IG++D ST+P    + PP  K ES A E   E++          K+L  L
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEK-ESTAAETTKEESGK--------KLLIYL 123

Query: 127 IPLLIIGVAFYLQYYGKK 145
           IPLLI+GVAF L++Y  K
Sbjct: 124 IPLLILGVAFALRFYNNK 132

[Close]

BLAST of CsGy3G007780.2 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 110.2 bits (274), Expect = 1.5e-24
Identity = 46/82 (56.10%), Postives = 62/82 (75.61%), Query Frame = 0


Query: 6  KLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFETVSHS 65
          KL+  +E A HN Q DCW++I GKVYDV+ ++++HPGGD+VLL    KDAT+DFE   HS
Sbjct: 6  KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66 LDATEEMEKYYIGNIDMSTIPK 88
           DA E MEKY+IG +D S++P+
Sbjct: 66 KDARELMEKYFIGELDESSLPE 87

The following BLAST results are available for this feature:

BLAST of CsGy3G007780.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P40934	7.6e-34	51.72	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
Q9ZWT2	4.9e-33	49.65	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]
Q42342	4.9e-33	50.69	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P49098	6.4e-33	49.31	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49099	1.6e-31	49.31	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]

BLAST of CsGy3G007780.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004133794.1	1.36e-95	100.00	cytochrome b5 [Cucumis sativus] >KGN56392.1 hypothetical protein Csa_010413 [Cuc...	[more]
XP_008437843.1	9.88e-82	88.97	PREDICTED: cytochrome b5-like [Cucumis melo]	[more]
TYK20812.1	5.86e-80	88.97	cytochrome b5-like [Cucumis melo var. makuwa]	[more]
KAA0048860.1	5.86e-80	88.97	cytochrome b5-like [Cucumis melo var. makuwa]	[more]
XP_038901262.1	3.86e-79	84.83	cytochrome b5-like [Benincasa hispida]	[more]

BLAST of CsGy3G007780.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L720	6.59e-96	100.00	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A1S3AV33	4.78e-82	88.97	cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103483149 PE=3 SV=1	[more]
A0A5A7U0D0	2.84e-80	88.97	Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G0...	[more]
A0A5D3DB41	2.84e-80	88.97	Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G0...	[more]
A0A6J1D2G0	3.41e-69	71.72	cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111016385 PE=3 SV=1	[more]

BLAST of CsGy3G007780.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53560.1	3.5e-34	50.69	cytochrome B5 isoform E	[more]
AT5G48810.1	3.5e-34	49.65	cytochrome B5 isoform D	[more]
AT2G32720.1	1.5e-32	48.95	cytochrome B5 isoform B	[more]
AT2G46650.1	1.4e-30	50.00	cytochrome B5 isoform C	[more]
AT1G26340.1	1.5e-24	56.10	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 55..62 score: 50.57 coord: 30..40 score: 69.15 coord: 68..80 score: 35.43 coord: 40..54 score: 47.47
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 1.1E-25 score: 101.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 5.3E-23 score: 80.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 26.852125
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..85 e-value: 1.1E-30 score: 107.4
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..91
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..110
None	No IPR available	PANTHER	PTHR19359:SF81	SUBFAMILY NOT NAMED	coord: 1..143
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..143
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CsGy3G007780	CsGy3G007780	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy3G007780.2.exon4	CsGy3G007780.2.exon4	exon
CsGy3G007780.2.exon3	CsGy3G007780.2.exon3	exon
CsGy3G007780.2.exon2	CsGy3G007780.2.exon2	exon
CsGy3G007780.2.exon1	CsGy3G007780.2.exon1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy3G007780.2.utr3p1	CsGy3G007780.2.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.CsGy3G007780.2	cds.CsGy3G007780.2_3	CDS
cds.CsGy3G007780.2	cds.CsGy3G007780.2_2	CDS
cds.CsGy3G007780.2	cds.CsGy3G007780.2	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy3G007780.2.utr5p2	CsGy3G007780.2.utr5p2	five_prime_UTR
CsGy3G007780.2.utr5p1	CsGy3G007780.2.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CsGy3G007780.2	CsGy3G007780.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding