CsGy3G002990.2 (mRNA) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G002990.2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Gy14Chr3: 2475561 .. 2477935 (-)
Sequence length	645
RNA-Seq Expression	CsGy3G002990.2
Synteny	CsGy3G002990.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATCCTCATACTTTCCTCTTCCAGTCAAAGCCATTCTTCTCATCCTCCTAATAAACCAATCAAACATTATTGCAAATTCTCAACCAATAAATGACACCCAATTCATCAAAACCACTTGCCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCTGCCGCCACCATTCACAGTAGCTGTCATCTAATGACCGTCGCTGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGATCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGATAGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTGTGGAGGAGTTGAAGGATAATAACAAGAGTCGATCCGAAACTGAAGATATTCGTACGTGGGTTAGTGCTGCTCTAACAGACGACGACACGTGTATGGATGGGTTGGTTGGAGATGCCATGAATGGCAACGTAAAGGAATCGATAAAGGAAATGGTGGTGAATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTGTAAGTTTGTTGAAGTGAGATTGAATGTGTAGCAAACATTTTGGCAAATACGTACTTGAGCATTATTTACATATTCAATTCATTTTGTCTATTTGATAGTTTTATAAAGTTTTTATTATATATCTAAATTTCAAGTACTTTAATCAAATTATTTATGAATGTATTTTATTTAAAACATTTTACCCACTTTTGTAGAGTTACATTTGTGTAATTATTGTTGTAATGTATGATATGGTTTACAATATAATAGTTAATTTTGTAACAACAAAAATTGAAGATATATACCCTATTATCGGTTAAAATATATAGCAACGCTAACTAAAATTTTCTAAAGATATTAAACAAGAAGATTAAAATTCGAAATCTAGAAATATCTTGATTTTAAGATAAAAAATTCTTTTGGATAAGAATAAGAGAAATATATAGAATTATGTAAATTAGTTAATCTAGAGAAGCTTTTTGGCTTAGACTCGAACGAATTCATGCCTATAACTAGGAAGTGTAATTTGTTAACCAAACAAATCAAAGTAAGTGAGAAAGAAAGAGAGAACGAGAGTTAGTAAATTTTTTTGGGTTAAAGTGAGTGTGAGGGTTCTTTCTTAAAGTGTTCATAATAAAACTTTCTTTCTTTGTGAGTCAAGCCAATTTTTTAGCATTATCTTCTATAATATAATTAGGCATTTTAAAATATCAACTCAATATTTTTATTCTTCAAAAAAGCATACAATTAGTTAAGTTTTAAAATAATTCTAACAAATTAAATAAATAAATTGTTTTACCAAATAAAACACAAACATGATTAATATGATTTTGTTAAATCATGCTGTAAAATTTCTTATAAATGATTTCCAAATAGGTTAATTTTTTGTAAATATAACAAAGTTAAAATATTTATTGCTCGTATAACATTATCAAAAAACTCATTAAGCTTACTATTTTAAATATCTTACATTTGTCCTTCATTTTTATTATCTTTCTTCTCTTCCTCTTGTGCAATCTTTTTTTTTTCTCTCTATCATCATTCTTCTCTTTTTTTGCGATTTTTCTTTCTTTTATTTTTTTCAAACTGCCATTTGATTCAAGAAGATCGTATCTTGAAAAAAAATGTTGTTTATTTAATCGACTAAATCTAAATGTCAAATATAAACGATCATATAATAATTAAAAATTGTTTAGATTTGACTATCCAAATTTAAATTAAATATAAACGATGGTAGGAAAAAATCTTGAAAAAAAATCATTTGATCAAATCTAAAACAATTGTGTAGGTCAGAATTAAATATATTAAGTGCACTGCATGTCAATTAATAACATGCCTCTGATATTTTTCAGTGTGAGTCTATAACATTTTTTAGTTTTTGAAAATTTTCTAGAGTGTAAATATTTTTTAATGGACATTTATAATTGATTTTCAATAATTATGTTTGGTTATTTTAAAATTATCAAACTATATTTGGGATTTGTAAATTATTTAATTGAATAAAACAAAACCACTAAAAAGACAGAATTTATTACACTTCATTTTTGTATAAAATGTAAATATTTTGTCAAATGTTTATTATTCACAAATACCCATTATAAAGTTTTACATTTGAAAATTATTTACAATAAATATGTTTTTTATTCATTTATAATTACATTTATTTTTTAAAAATATTTTTATTAAAAAGTTGGCCACAAACAATTGGTGAAAAATGTAGTTGGAAATGGTAGCCGGCGATGGTGATTAGAGGTTCGCAACATTGGTCGTTGAAGTTCATCAAAGGGTTGGCAACGAAGCTGTGGCGAAGCGGGGACACTCTCCATCCCCGTGA

mRNA sequence

ATGAAATCCTCATACTTTCCTCTTCCAGTCAAAGCCATTCTTCTCATCCTCCTAATAAACCAATCAAACATTATTGCAAATTCTCAACCAATAAATGACACCCAATTCATCAAAACCACTTGCCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCTGCCGCCACCATTCACAGTAGCTGTCATCTAATGACCGTCGCTGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGATCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGATAGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTGTGGAGGAGTTGAAGGATAATAACAAGAGTCGATCCGAAACTGAAGATATTCGTACGTGGGTTAGTGCTGCTCTAACAGACGACGACACGTGTATGGATGGGTTGGTTGGAGATGCCATGAATGGCAACGTAAAGGAATCGATAAAGGAAATGGTGGTGAATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTAGGTTCGCAACATTGGTCGTTGAAGTTCATCAAAGGGTTGGCAACGAAGCTGTGGCGAAGCGGGGACACTCTCCATCCCCGTGA

Coding sequence (CDS)

ATGAAATCCTCATACTTTCCTCTTCCAGTCAAAGCCATTCTTCTCATCCTCCTAATAAACCAATCAAACATTATTGCAAATTCTCAACCAATAAATGACACCCAATTCATCAAAACCACTTGCCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCTGCCGCCACCATTCACAGTAGCTGTCATCTAATGACCGTCGCTGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGATCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGATAGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTGTGGAGGAGTTGAAGGATAATAACAAGAGTCGATCCGAAACTGAAGATATTCGTACGTGGGTTAGTGCTGCTCTAACAGACGACGACACGTGTATGGATGGGTTGGTTGGAGATGCCATGAATGGCAACGTAAAGGAATCGATAAAGGAAATGGTGGTGAATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTAGGTTCGCAACATTGGTCGTTGAAGTTCATCAAAGGGTTGGCAACGAAGCTGTGGCGAAGCGGGGACACTCTCCATCCCCGTGA

Protein sequence

MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLRFATLVVEVHQRVGNEAVAKRGHSPSP*

Homology

[Close]

BLAST of CsGy3G002990.2 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.4e-22
Identity = 76/192 (39.58%), Postives = 108/192 (56.25%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           MK S   L   A+L+IL        A++ P N  QFIKT+C  T YP +C  SLS  A T
Sbjct: 1   MKLSKSTLVFSALLVIL------AAASAAPAN--QFIKTSCTLTTYPAVCEQSLSAYAKT 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           I ++   +   AL V+LT T+   + ++ L K    L  R    I DC+EE  DS++ + 
Sbjct: 61  IQNNPQELASTALQVSLTRTQQAQTFMKRLNK-FKGLKARQYAAIHDCLEEVEDSLDRVS 120

Query: 121 MAVEELKD-----NNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVV 180
            + +E+K+      N       ++ TWVSAALTD+ TCMDG  G  M+G +KES++  VV
Sbjct: 121 RSCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVV 180

Query: 181 NVAQLTSIALSL 188
            VA++TS AL+L
Sbjct: 181 AVARVTSNALAL 183

[Close]

BLAST of CsGy3G002990.2 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.3e-21
Identity = 65/185 (35.14%), Postives = 102/185 (55.14%), Query Frame = 0


Query: 13  ILLILLINQSNII----ANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLM 72
           IL +L ++ + I+       +   DT+FIK +C++T YPD C  SLS  A+ I      +
Sbjct: 10  ILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKL 69

Query: 73  TVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD 132
              AL V++   +S  + +  +      +T R    + DC+EE GD+V+ L  +++ELK 
Sbjct: 70  AETALAVSIARAKSAKTYVSEMT-DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKH 129

Query: 133 NNKSRSETE------DIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTS 188
             +  S  +      ++RTW SAALTD+  CMDG  G AM G +K  I+  +V+VA+ TS
Sbjct: 130 LEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETS 189

[Close]

BLAST of CsGy3G002990.2 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.1e-20
Identity = 72/182 (39.56%), Postives = 108/182 (59.34%), Query Frame = 0


Query: 13  ILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATI-HSSCHLMTVA 72
           ILL + I QS+    ++  +D QFI ++CQ+T YP LC+ +LS  A  I H++   +   
Sbjct: 11  ILLSITILQSSSATPNRSESD-QFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQT 70

Query: 73  ALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEEL-KDNN 132
           AL ++L   +S +  +  L K +     R+   I+DCIE  G+SV+ L  +V+EL +  +
Sbjct: 71  ALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGH 130

Query: 133 KSRSE-----TEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIAL 188
              SE       +++TWVSAALTD+ TC+DG    AM G VK  I+  VV+VAQ+TS AL
Sbjct: 131 AVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNAL 190

[Close]

BLAST of CsGy3G002990.2 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.6e-19
Identity = 70/186 (37.63%), Postives = 106/186 (56.99%), Query Frame = 0


Query: 7   PLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCH 66
           P P  + L  L  + S+   ++Q   +  +IKT+C  T Y  +C +SLS  A+TI S+  
Sbjct: 120 PPPSSSPLSSLSPSSSSSTYSNQ--TNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQ 179

Query: 67  LMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEEL 126
            + V AL + L+  +S S  +++++     LT  +   + DC+EE GDSV  L+ ++ EL
Sbjct: 180 KLAVIALNLTLSSAKSASKFVKNIS-HGGGLTRLEVVAVADCVEEIGDSVTSLQDSIREL 239

Query: 127 -----KDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLT 186
                KD+ K      D+ TWVSAALT+DDTCMDG     +   VK+ ++  VV VA+LT
Sbjct: 240 DSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLT 299

Query: 187 SIALSL 188
           S AL+L
Sbjct: 300 SNALAL 300

[Close]

BLAST of CsGy3G002990.2 vs. ExPASy Swiss-Prot
Match: Q9SB38 (Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 6.6e-18
Identity = 58/187 (31.02%), Postives = 104/187 (55.61%), Query Frame = 0


Query: 10  VKAILLILLINQSNI--IANSQP---INDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSS 69
           V ++ L++ IN SN    A + P    N T ++KT C ST YP +C + LS  ++TI S 
Sbjct: 6   VLSLTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKSD 65

Query: 70  CHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVE 129
              +   +L + +   ++ +  + +L + + A    +  +++DC++E  D+++ELK AV 
Sbjct: 66  PIKLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVA 125

Query: 130 ELKDNNKSRSETED----IRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 188
           E+K        TE+    ++TWVS+ALTD+ TC DG     +N   K+ +K+ +  +++ 
Sbjct: 126 EMKYVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEGRVNVETKKKVKKAISELSKT 185

[Close]

BLAST of CsGy3G002990.2 vs. NCBI nr
Match: XP_004147977.1 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 345 bits (885), Expect = 1.12e-118
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT
Sbjct: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK
Sbjct: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120

Query: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180
           MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL
Sbjct: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180

Query: 181 TSIALSL 187
           TSIALSL
Sbjct: 181 TSIALSL 187

[Close]

BLAST of CsGy3G002990.2 vs. NCBI nr
Match: XP_008448897.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 310 bits (793), Expect = 1.16e-104
Identity = 170/188 (90.43%), Postives = 179/188 (95.21%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLIL-LINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAA 60
           MKSSYFPLPVKAILL++ LINQSNI ANSQP NDTQFIKTTCQSTPYPDLCLSSLSDSAA
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNI-ANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAA 60

Query: 61  TIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEEL 120
            IHSS  LMT+AALTVALTHTRSTSSA+ESLAKSSNALTPR+ YVIRDCIEEFGDSVEEL
Sbjct: 61  AIHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEEL 120

Query: 121 KMAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQ 180
           KMA+EELKDNNK+R ETEDIRTWVSAALTDDDTCMDGLVGDAMNGN+KESIK MVV+VAQ
Sbjct: 121 KMAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQ 180

Query: 181 LTSIALSL 187
           LTSIALSL
Sbjct: 181 LTSIALSL 187

[Close]

BLAST of CsGy3G002990.2 vs. NCBI nr
Match: KAE8650073.1 (hypothetical protein Csa_009584 [Cucumis sativus])

HSP 1 Score: 273 bits (699), Expect = 5.10e-91
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 68  MTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELK 127
           MTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELK
Sbjct: 1   MTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELK 60

Query: 128 DNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSL 187
           DNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSL
Sbjct: 61  DNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSL 120

Query: 188 RFATLVVEVHQRVGNEAVAKRGHSPSP 214
           RFATLVVEVHQRVGNEAVAKRGHSPSP
Sbjct: 121 RFATLVVEVHQRVGNEAVAKRGHSPSP 147

[Close]

BLAST of CsGy3G002990.2 vs. NCBI nr
Match: XP_038876812.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 267 bits (683), Expect = 6.02e-88
Identity = 147/187 (78.61%), Postives = 163/187 (87.17%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           M+SSY PLP+KAILLI+LINQSNI A SQPINDTQFIKTTCQSTPYP LCLSSLS  AAT
Sbjct: 1   MESSYLPLPIKAILLIILINQSNI-AKSQPINDTQFIKTTCQSTPYPSLCLSSLSADAAT 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           IH S  LMT+AALTVALT TRS SS I+SLAKS N LTPR+ YV+ DCIEEFGDS+EELK
Sbjct: 61  IHGSYRLMTIAALTVALTRTRSASSVIQSLAKS-NVLTPREGYVVSDCIEEFGDSIEELK 120

Query: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180
           MA+ ELKDN+K R E EDIRTWVSAALTDDDTCMDG VGD MN +VKES+K++VV++ QL
Sbjct: 121 MAIVELKDNDKMRFEIEDIRTWVSAALTDDDTCMDGFVGDTMNDSVKESMKKLVVDIVQL 180

Query: 181 TSIALSL 187
           TSIALSL
Sbjct: 181 TSIALSL 185

[Close]

BLAST of CsGy3G002990.2 vs. NCBI nr
Match: XP_023551923.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 235 bits (600), Expect = 2.35e-75
Identity = 129/180 (71.67%), Postives = 152/180 (84.44%), Query Frame = 0


Query: 8   LPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHL 67
           LP+ AIL ++LINQS  +A +QPINDTQFIKTTCQ+TPYPDLCLSSLS  A+ IH S  L
Sbjct: 6   LPITAILFMILINQSYTVA-TQPINDTQFIKTTCQTTPYPDLCLSSLSADASAIHGSHRL 65

Query: 68  MTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELK 127
           MT+AAL VALTHT   SS + SLAKS NALTPR+ Y + DCIEEFGDS+EELKMAVEELK
Sbjct: 66  MTIAALAVALTHTHDASSTVTSLAKS-NALTPREGYALSDCIEEFGDSIEELKMAVEELK 125

Query: 128 DNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSL 187
            N+K+R + +DI+TWVSAALTDD+TCMDG   DAMNG+VKES++ +VV+VAQLTSIALSL
Sbjct: 126 GNDKTRLDIKDIQTWVSAALTDDNTCMDGFAEDAMNGSVKESVRALVVDVAQLTSIALSL 183

[Close]

BLAST of CsGy3G002990.2 vs. ExPASy TrEMBL
Match: A0A0A0L1P3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 SV=1)

HSP 1 Score: 345 bits (885), Expect = 5.44e-119
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT
Sbjct: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK
Sbjct: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120

Query: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180
           MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL
Sbjct: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180

Query: 181 TSIALSL 187
           TSIALSL
Sbjct: 181 TSIALSL 187

[Close]

BLAST of CsGy3G002990.2 vs. ExPASy TrEMBL
Match: A0A1S3BLP3 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1)

HSP 1 Score: 310 bits (793), Expect = 5.63e-105
Identity = 170/188 (90.43%), Postives = 179/188 (95.21%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLIL-LINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAA 60
           MKSSYFPLPVKAILL++ LINQSNI ANSQP NDTQFIKTTCQSTPYPDLCLSSLSDSAA
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNI-ANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAA 60

Query: 61  TIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEEL 120
            IHSS  LMT+AALTVALTHTRSTSSA+ESLAKSSNALTPR+ YVIRDCIEEFGDSVEEL
Sbjct: 61  AIHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEEL 120

Query: 121 KMAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQ 180
           KMA+EELKDNNK+R ETEDIRTWVSAALTDDDTCMDGLVGDAMNGN+KESIK MVV+VAQ
Sbjct: 121 KMAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQ 180

Query: 181 LTSIALSL 187
           LTSIALSL
Sbjct: 181 LTSIALSL 187

[Close]

BLAST of CsGy3G002990.2 vs. ExPASy TrEMBL
Match: A0A6J1E6H9 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1)

HSP 1 Score: 234 bits (597), Expect = 3.25e-75
Identity = 129/180 (71.67%), Postives = 151/180 (83.89%), Query Frame = 0


Query: 8   LPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHL 67
           LP+ AI+ ++LINQS  +A +QPINDTQFIKTTCQ+TPYPDLCLSSLS  A+ I  S  L
Sbjct: 6   LPITAIVFVILINQSYTVA-TQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRL 65

Query: 68  MTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELK 127
           MT+AAL VALTHT   SS + SLAKS NA+T R+ Y + DCIEEFGDSVEELKMAVE LK
Sbjct: 66  MTIAALAVALTHTHDASSTVTSLAKS-NAITTREGYALSDCIEEFGDSVEELKMAVEALK 125

Query: 128 DNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSL 187
            N+K+R +TEDI+TW+SAALTDD+TCMDG  GDAMNG+VKES+K MVV+VAQLTSIALSL
Sbjct: 126 GNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSL 183

[Close]

BLAST of CsGy3G002990.2 vs. ExPASy TrEMBL
Match: A0A6J1DFW8 (pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 PE=4 SV=1)

HSP 1 Score: 226 bits (576), Expect = 4.41e-72
Identity = 128/187 (68.45%), Postives = 151/187 (80.75%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           M+ SYFPLP+ AILLI+LIN SN       I+DTQFIK TCQ+TPYPDLC SSLS  A +
Sbjct: 1   MEDSYFPLPITAILLIILINLSN-----ATIDDTQFIKATCQTTPYPDLCFSSLSADAVS 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           +H S   MT+AALTVALTHTRS SS + SLAKS NAL P++++ +RDCIEE GDSVEEL+
Sbjct: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAKS-NALAPQETWALRDCIEELGDSVEELQ 120

Query: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180
            +V ELKD + SR ET+DIRTWVSAALTDDDTCM+GLVGD   G VKES++ +VV+VAQL
Sbjct: 121 SSVAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDG--GGVKESVRGLVVDVAQL 179

Query: 181 TSIALSL 187
           TSIALSL
Sbjct: 181 TSIALSL 179

[Close]

BLAST of CsGy3G002990.2 vs. ExPASy TrEMBL
Match: A0A5D3D7I0 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001400 PE=4 SV=1)

HSP 1 Score: 209 bits (531), Expect = 4.35e-66
Identity = 110/120 (91.67%), Postives = 117/120 (97.50%), Query Frame = 0


Query: 68  MTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELK 127
           MT+AALTVALTHTRSTSSA+ESLAKSSNALTPR+ YVIRDCIEEFGDSVEELKMA+EELK
Sbjct: 1   MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK 60

Query: 128 DNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSL 187
           DNNK+R ETEDIRTWVSAALTDDDTCMDGLVGDAMNGN+KESIK MVV+VAQLTSIALSL
Sbjct: 61  DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSL 120

[Close]

BLAST of CsGy3G002990.2 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 112.5 bits (280), Expect = 4.3e-25
Identity = 70/177 (39.55%), Postives = 109/177 (61.58%), Query Frame = 0


Query: 13  ILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAA 72
           I+ +L +  +     +    +T+F+K++C  T YP LC SSLS  A+ I +S  LM  AA
Sbjct: 20  IIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAA 79

Query: 73  LTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKDNNKS 132
           L + L   + TS+ +  L  S++ L P++   +RDC+EE GD++EEL+ ++ E+   + S
Sbjct: 80  LNITLASAKVTSAMMVRL--SNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGS 139

Query: 133 RSET--EDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSL 188
             E    DI+TWVSAALTD +TC DG  G+ M+G VK  ++  ++ +A LTS AL+L
Sbjct: 140 NYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALAL 194

[Close]

BLAST of CsGy3G002990.2 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 4.5e-22
Identity = 65/185 (35.14%), Postives = 102/185 (55.14%), Query Frame = 0


Query: 13  ILLILLINQSNII----ANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLM 72
           IL +L ++ + I+       +   DT+FIK +C++T YPD C  SLS  A+ I      +
Sbjct: 10  ILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKL 69

Query: 73  TVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD 132
              AL V++   +S  + +  +      +T R    + DC+EE GD+V+ L  +++ELK 
Sbjct: 70  AETALAVSIARAKSAKTYVSEMT-DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKH 129

Query: 133 NNKSRSETE------DIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTS 188
             +  S  +      ++RTW SAALTD+  CMDG  G AM G +K  I+  +V+VA+ TS
Sbjct: 130 LEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETS 189

[Close]

BLAST of CsGy3G002990.2 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.9e-21
Identity = 72/182 (39.56%), Postives = 108/182 (59.34%), Query Frame = 0


Query: 13  ILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATI-HSSCHLMTVA 72
           ILL + I QS+    ++  +D QFI ++CQ+T YP LC+ +LS  A  I H++   +   
Sbjct: 11  ILLSITILQSSSATPNRSESD-QFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQT 70

Query: 73  ALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEEL-KDNN 132
           AL ++L   +S +  +  L K +     R+   I+DCIE  G+SV+ L  +V+EL +  +
Sbjct: 71  ALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGH 130

Query: 133 KSRSE-----TEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIAL 188
              SE       +++TWVSAALTD+ TC+DG    AM G VK  I+  VV+VAQ+TS AL
Sbjct: 131 AVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNAL 190

[Close]

BLAST of CsGy3G002990.2 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 98.6 bits (244), Expect = 6.5e-21
Identity = 63/168 (37.50%), Postives = 97/168 (57.74%), Query Frame = 0


Query: 26  ANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAALTVALTHTRSTSS 85
           A+ + IN   FI+++C++T YP LC+ SLS  A  I +S   +   A+ V L+  +ST  
Sbjct: 31  ASKKAIN---FIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKL 90

Query: 86  AIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEEL------KDNNKSRSETEDI 145
            +  L +    L  R+   I+DC+EE  D+V+ L  +V+EL      KD ++      + 
Sbjct: 91  FVSRLTR-MKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNA 150

Query: 146 RTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSL 188
           +TW SAALTD++TC DG  G  M+G +K S++  ++NV   TS ALSL
Sbjct: 151 QTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSL 194

[Close]

BLAST of CsGy3G002990.2 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-20
Identity = 70/186 (37.63%), Postives = 106/186 (56.99%), Query Frame = 0


Query: 7   PLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCH 66
           P P  + L  L  + S+   ++Q   +  +IKT+C  T Y  +C +SLS  A+TI S+  
Sbjct: 120 PPPSSSPLSSLSPSSSSSTYSNQ--TNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQ 179

Query: 67  LMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEEL 126
            + V AL + L+  +S S  +++++     LT  +   + DC+EE GDSV  L+ ++ EL
Sbjct: 180 KLAVIALNLTLSSAKSASKFVKNIS-HGGGLTRLEVVAVADCVEEIGDSVTSLQDSIREL 239

Query: 127 -----KDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLT 186
                KD+ K      D+ TWVSAALT+DDTCMDG     +   VK+ ++  VV VA+LT
Sbjct: 240 DSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLT 299

Query: 187 SIALSL 188
           S AL+L
Sbjct: 300 SNALAL 300

The following BLAST results are available for this feature:

BLAST of CsGy3G002990.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	3.4e-22	39.58	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB37	6.3e-21	35.14	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI72	4.1e-20	39.56	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SI74	1.6e-19	37.63	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9SB38	6.6e-18	31.02	Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1	[more]

BLAST of CsGy3G002990.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147977.1	1.12e-118	100.00	21 kDa protein [Cucumis sativus]	[more]
XP_008448897.1	1.16e-104	90.43	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
KAE8650073.1	5.10e-91	100.00	hypothetical protein Csa_009584 [Cucumis sativus]	[more]
XP_038876812.1	6.02e-88	78.61	21 kDa protein-like [Benincasa hispida]	[more]
XP_023551923.1	2.35e-75	71.67	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsGy3G002990.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1P3	5.44e-119	100.00	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 S...	[more]
A0A1S3BLP3	5.63e-105	90.43	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1	[more]
A0A6J1E6H9	3.25e-75	71.67	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1	[more]
A0A6J1DFW8	4.41e-72	68.45	pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 P...	[more]
A0A5D3D7I0	4.35e-66	91.67	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G...	[more]

BLAST of CsGy3G002990.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	4.3e-25	39.55	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	4.5e-22	35.14	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	2.9e-21	39.56	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	6.5e-21	37.50	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	1.1e-20	37.63	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 109..136
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 19..188
None	No IPR available	PANTHER	PTHR31080:SF161	21 KDA PROTEIN-LIKE	coord: 19..188
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 39..187 e-value: 3.9537E-36 score: 122.547
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 32..187 e-value: 1.4E-43 score: 160.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 13..187 e-value: 1.6E-31 score: 107.0
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 36..187 e-value: 2.4E-35 score: 122.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 31..189 e-value: 3.7E-37 score: 129.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 35..187

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CsGy3G002990	CsGy3G002990	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy3G002990.2.exon2	CsGy3G002990.2.exon2	exon
CsGy3G002990.2.exon1	CsGy3G002990.2.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.CsGy3G002990.2	cds.CsGy3G002990.2_2	CDS
cds.CsGy3G002990.2	cds.CsGy3G002990.2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CsGy3G002990.2	CsGy3G002990.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity