CsGy3G001930.1 (mRNA) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G001930.1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	protein N-lysine methyltransferase METTL21A-like
Location	Gy14Chr3: 1490640 .. 1491305 (-)
Sequence length	666
RNA-Seq Expression	CsGy3G001930.1
Synteny	CsGy3G001930.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTTAAAGAGTTCCAAATCAGTGATCGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGAAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTCGTCCTAGCCAAGTGGATGGCAACTCAGTGCAACCTATTCGACTTCGACTTCCGGCAGAAGAACGTAATCGAGCTTGGCGCTGGGACTGGACTTCCTGGTTTGACAGCAGCTTTACTTGGTGCTAATCGTGTTCTACTTACCGATGTAGAGCCATTGCTTCCTGGGTTGTTGGAGAATGTAGATGCTAATGGAGTTGGCGATCGAGTGGAAGTAAGGGAACTCGTGTGGGGCTCAAACGACTTGCCAAGTCAAGCAAATGAGTTGGGGGAGTTTGACCTGATTTTGATGTCAGATTTGTTCTACAACTCGGAGGAAATGCCCCATCTAGCACAAGTACTAAAGATCATCTCTGGGACAGATACCAAAATATGGGCAGCATCGGAGATCCGACCATGGACTATTGAATGCATTACAGAGTTGATAAATCAAGGATTCAAGATCACAGAGTCCCCAGTTCAACATGATGAATCTCATGAGGAAGACAACCATAAGATTTTGGACTCGTTTTCTATATTCCATCTCAGACAAGAAGAAAGAAACCTTTAA

mRNA sequence

ATGGTTGTTAAAGAGTTCCAAATCAGTGATCGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGAAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTCGTCCTAGCCAAGTGGATGGCAACTCAGTGCAACCTATTCGACTTCGACTTCCGGCAGAAGAACGTAATCGAGCTTGGCGCTGGGACTGGACTTCCTGGTTTGACAGCAGCTTTACTTGGTGCTAATCGTGTTCTACTTACCGATGTAGAGCCATTGCTTCCTGGGTTGTTGGAGAATGTAGATGCTAATGGAGTTGGCGATCGAGTGGAAGTAAGGGAACTCGTGTGGGGCTCAAACGACTTGCCAAGTCAAGCAAATGAGTTGGGGGAGTTTGACCTGATTTTGATGTCAGATTTGTTCTACAACTCGGAGGAAATGCCCCATCTAGCACAAGTACTAAAGATCATCTCTGGGACAGATACCAAAATATGGGCAGCATCGGAGATCCGACCATGGACTATTGAATGCATTACAGAGTTGATAAATCAAGGATTCAAGATCACAGAGTCCCCAGTTCAACATGATGAATCTCATGAGGAAGACAACCATAAGATTTTGGACTCGTTTTCTATATTCCATCTCAGACAAGAAGAAAGAAACCTTTAA

Coding sequence (CDS)

ATGGTTGTTAAAGAGTTCCAAATCAGTGATCGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGAAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTCGTCCTAGCCAAGTGGATGGCAACTCAGTGCAACCTATTCGACTTCGACTTCCGGCAGAAGAACGTAATCGAGCTTGGCGCTGGGACTGGACTTCCTGGTTTGACAGCAGCTTTACTTGGTGCTAATCGTGTTCTACTTACCGATGTAGAGCCATTGCTTCCTGGGTTGTTGGAGAATGTAGATGCTAATGGAGTTGGCGATCGAGTGGAAGTAAGGGAACTCGTGTGGGGCTCAAACGACTTGCCAAGTCAAGCAAATGAGTTGGGGGAGTTTGACCTGATTTTGATGTCAGATTTGTTCTACAACTCGGAGGAAATGCCCCATCTAGCACAAGTACTAAAGATCATCTCTGGGACAGATACCAAAATATGGGCAGCATCGGAGATCCGACCATGGACTATTGAATGCATTACAGAGTTGATAAATCAAGGATTCAAGATCACAGAGTCCCCAGTTCAACATGATGAATCTCATGAGGAAGACAACCATAAGATTTTGGACTCGTTTTCTATATTCCATCTCAGACAAGAAGAAAGAAACCTTTAA

Protein sequence

MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSNDLPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITELINQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL*

Homology

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BLAST of CsGy3G001930.1 vs. ExPASy Swiss-Prot
Match: Q5BLD8 (Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.4e-18
Identity = 69/169 (40.83%), Postives = 89/169 (52.66%), Query Frame = 0


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A VL  ++     +   D + K VIELGAGTGL G+ AALLGAN V +TD EP L 
Sbjct: 46  VWDAAVVLCMFL----EMGKVDLKGKRVIELGAGTGLVGIVAALLGAN-VTITDREPALE 105

Query: 95  GLLENVDAN---GVGDRVEVRELVWGSN-DLPSQANELGEFDLILMSDLFYNSEEMPHLA 154
            L  NV  N   G    V+V EL WG N DL  Q    G +DLIL +D+ Y  E  P L 
Sbjct: 106 FLTANVHENIPQGRQKAVQVSELTWGENLDLYPQ----GGYDLILGADIVYLEETFPALL 165

Query: 155 QVLKIISGTDTKIWAASEIRPWTIECITELINQGFKITESPVQHDESHE 200
           Q L+ +S  DT +  +  IR    E     + Q F + E  V +D   +
Sbjct: 166 QTLEHLSSGDTVVLLSCRIRYERDERFLTELRQRFSVQE--VHYDSQRD 203

[Close]

BLAST of CsGy3G001930.1 vs. ExPASy Swiss-Prot
Match: A4FV98 (EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 7.0e-15
Identity = 59/166 (35.54%), Postives = 88/166 (53.01%), Query Frame = 0


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A  L  +  +Q    + DFR K VIELGAGTG+ G+ AAL G + V +TD+  +L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLVLE 115

Query: 95  GLLENVDAN-GVGDRVEVRELVWGSNDLPSQANELGEFDLILMSDLFYNSEEMPHLAQVL 154
            +  NV AN   G R +VR L WG +    Q    G++DL+L +D+ Y     P L   L
Sbjct: 116 QIQGNVQANVPPGGRAQVRALSWGID----QHVFPGDYDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KIISGTDTKIWAASEIRP--WTIECITELINQGFKITESPVQHDES 198
           + + G    I+ AS++R    T      L+ Q F++  +    DE+
Sbjct: 176 RHLCGPHGTIYLASKMREEHGTESFFQHLLPQHFQLELAQRDEDEN 212

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BLAST of CsGy3G001930.1 vs. ExPASy Swiss-Prot
Match: Q8C436 (Protein-lysine methyltransferase METTL21D OS=Mus musculus OX=10090 GN=Vcpkmt PE=2 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 1.2e-14
Identity = 66/183 (36.07%), Postives = 96/183 (52.46%), Query Frame = 0


Query: 32  GSWVWNSAFVLAKWMATQCNLFD--FDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDV 91
           G  VW++A VL+K++ T     D      +++V+ELG+GTG  GL AA LGA+ V++TD+
Sbjct: 39  GCVVWDAAIVLSKYLETPGFSGDGAHALSRRSVLELGSGTGAVGLMAATLGAD-VIVTDL 98

Query: 92  EPLLPGLLENVDANG--VGDRVEVRELVWGSNDLPSQANELGEFDLILMSDLFYNSEEMP 151
           E L   L  N+D N   V   V+ + L WG +       +L   D ILM+D  Y  E + 
Sbjct: 99  EELQDLLKMNIDMNKHLVTGSVQAKVLKWGED-----IEDLMSPDYILMADCIYYEESLE 158

Query: 152 HLAQVLKIISGTDTKIWAASEIR-----PWTIECITELINQGFKITESPV-QHDESH-EE 204
            L + LK +SG++T I    E R     P   +   EL+   F   E P+ +HDE +  E
Sbjct: 159 PLLKTLKDLSGSETCIICCYEQRTMGKNPEIEKKYFELLQLDFDFEEIPLDKHDEEYRSE 215

[Close]

BLAST of CsGy3G001930.1 vs. ExPASy Swiss-Prot
Match: Q8WXB1 (Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A PE=1 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 4.5e-14
Identity = 54/139 (38.85%), Postives = 75/139 (53.96%), Query Frame = 0


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A VL    +T   +   + R ++ +ELGAGTGL G+ AALLGA+ V +TD +  L 
Sbjct: 46  VWDAAIVL----STYLEMGAVELRGRSAVELGAGTGLVGIVAALLGAH-VTITDRKVALE 105

Query: 95  GLLENVDAN---GVGDRVEVRELVWGSNDLPSQANELGEFDLILMSDLFYNSEEMPHLAQ 154
            L  NV AN    +  +  V+EL WG N     +   GEFDLIL +D+ Y  E    L Q
Sbjct: 106 FLKSNVQANLPPHIQTKTVVKELTWGQN---LGSFSPGEFDLILGADIIYLEETFTDLLQ 165

Query: 155 VLKIISGTDTKIWAASEIR 171
            L+ +    + I  A  IR
Sbjct: 166 TLEHLCSNHSVILLACRIR 176

[Close]

BLAST of CsGy3G001930.1 vs. ExPASy Swiss-Prot
Match: D3YWP0 (EEF1A lysine methyltransferase 3 OS=Mus musculus OX=10090 GN=eef1akmt3 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.7e-13
Identity = 56/156 (35.90%), Postives = 82/156 (52.56%), Query Frame = 0


Query: 16  HELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLT 75
           HEL  +  +   R+   + VW++A  L  +  +Q    + DFR K VIELGAGTG+ G+ 
Sbjct: 38  HEL-SITQNFGSRLGVAARVWDAALSLCDYFESQ----NVDFRGKKVIELGAGTGIVGIL 97

Query: 76  AALLGANRVLLTDVEPLLPGLLENVDAN-GVGDRVEVRELVWGSNDLPSQANELGEFDLI 135
           AAL G + V +TD+   L  + +NV AN   G R  V  L WG +    Q    G +DL+
Sbjct: 98  AALQGGD-VTITDLPVALEQIQDNVHANVPPGGRARVCALSWGID----QHVFPGNYDLV 157

Query: 136 LMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIR 171
           L +D+ Y     P L   L+ + G    I+ AS++R
Sbjct: 158 LGADIVYLEPTFPLLLGTLRHLCGPHGTIYLASKMR 183

[Close]

BLAST of CsGy3G001930.1 vs. NCBI nr
Match: XP_004150176.1 (protein N-lysine methyltransferase METTL21A [Cucumis sativus])

HSP 1 Score: 449 bits (1155), Expect = 2.91e-159
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND
Sbjct: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120

Query: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180
           LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL
Sbjct: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 221
           INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 221

[Close]

BLAST of CsGy3G001930.1 vs. NCBI nr
Match: XP_008448745.1 (PREDICTED: protein N-lysine methyltransferase METTL21A-like [Cucumis melo] >KAA0053074.1 protein N-lysine methyltransferase METTL21A-like [Cucumis melo var. makuwa])

HSP 1 Score: 416 bits (1069), Expect = 3.91e-146
Identity = 206/222 (92.79%), Postives = 215/222 (96.85%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFD RQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAG GLPGLTAALLGANRV+LTDV+PLL GLLENVDAN +GDRVEVRELVWGSN+
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180
           L SQANELGEFDLILMSDLFY+SEEMPHLAQVLKIISGTD+KIWAASEIRPWTIECITEL
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQHD-ESHEEDNHKILDSFSIFHLRQEERNL 221
           INQGFKITESPVQHD ESHEEDNHK+LDS+SIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLRQEERNL 222

[Close]

BLAST of CsGy3G001930.1 vs. NCBI nr
Match: XP_022923423.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata])

HSP 1 Score: 350 bits (897), Expect = 6.80e-120
Identity = 174/223 (78.03%), Postives = 195/223 (87.44%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M V+EFQISD N+VIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFDFR K
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
            VIELGAG GLPGL AALLGA RV+LTDVEPLLPGLL NVDANG+ DRVEVRELVWG ++
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180
             S+ANE GEFDLILMSDLFY+SE+M  LA +LKIISG ++KIWAASEIRPWT+EC+TE 
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 INQGFKITESPVQHDE-SHEEDNHKILDSFSIFHLR--QEERN 220
           +N+GFK+ ES VQ DE S E+D+H ILDSFSIFHL+   EERN
Sbjct: 181 MNEGFKVEESQVQLDEPSEEDDDHAILDSFSIFHLQPPDEERN 223

[Close]

BLAST of CsGy3G001930.1 vs. NCBI nr
Match: XP_023553140.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350 bits (897), Expect = 1.52e-119
Identity = 175/223 (78.48%), Postives = 193/223 (86.55%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M V+EFQISDRN+VIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFDFR K
Sbjct: 24  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 83

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
            VIELGAG GLPGL AALLGA RV+LTDVEPLLPGLL NVDANG+ DRVEVRELVWG ++
Sbjct: 84  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 143

Query: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180
             S+ANE GEFDLILMSDLFY+SE+M  LA +LKIISG  +KIWAASEIRPWT+EC+TE 
Sbjct: 144 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKHSKIWAASEIRPWTVECLTEF 203

Query: 181 INQGFKITESPVQHDESHEEDN-HKILDSFSIFHLR--QEERN 220
            N+GFK+ ES VQ DE  EED+ H ILDSFSIFHL+   EERN
Sbjct: 204 TNEGFKVEESQVQLDEPREEDDDHTILDSFSIFHLQPPDEERN 246

[Close]

BLAST of CsGy3G001930.1 vs. NCBI nr
Match: KAG7015681.1 (EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 347 bits (891), Expect = 5.57e-119
Identity = 173/223 (77.58%), Postives = 194/223 (87.00%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M V+EFQISD N+VIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMAT+C LFDFDFR K
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATRCKLFDFDFRDK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
            VIELGAG GLPGL AALLGA RV+LTDVEPLLPGLL NVDANG+ DRVEVRELVWG ++
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180
             S+ANE GEFDLILMSDLFY+SE+M  LA +LKIISG ++KIWAASEIRPWT+EC+TE 
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 INQGFKITESPVQHDESHEE-DNHKILDSFSIFHLR--QEERN 220
           +N+GFK+ ES VQ DE  EE D+H ILDSFSIFHL+   EERN
Sbjct: 181 MNEGFKVEESQVQLDEPREEVDDHAILDSFSIFHLQPPDEERN 223

[Close]

BLAST of CsGy3G001930.1 vs. ExPASy TrEMBL
Match: A0A0A0L6N4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016950 PE=4 SV=1)

HSP 1 Score: 449 bits (1155), Expect = 1.41e-159
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND
Sbjct: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120

Query: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180
           LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL
Sbjct: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 221
           INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 221

[Close]

BLAST of CsGy3G001930.1 vs. ExPASy TrEMBL
Match: A0A5A7UCU5 (Protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G001920 PE=4 SV=1)

HSP 1 Score: 416 bits (1069), Expect = 1.89e-146
Identity = 206/222 (92.79%), Postives = 215/222 (96.85%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFD RQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAG GLPGLTAALLGANRV+LTDV+PLL GLLENVDAN +GDRVEVRELVWGSN+
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180
           L SQANELGEFDLILMSDLFY+SEEMPHLAQVLKIISGTD+KIWAASEIRPWTIECITEL
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQHD-ESHEEDNHKILDSFSIFHLRQEERNL 221
           INQGFKITESPVQHD ESHEEDNHK+LDS+SIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLRQEERNL 222

[Close]

BLAST of CsGy3G001930.1 vs. ExPASy TrEMBL
Match: A0A1S3BKF2 (protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo OX=3656 GN=LOC103490817 PE=4 SV=1)

HSP 1 Score: 416 bits (1069), Expect = 1.89e-146
Identity = 206/222 (92.79%), Postives = 215/222 (96.85%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFD RQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAG GLPGLTAALLGANRV+LTDV+PLL GLLENVDAN +GDRVEVRELVWGSN+
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180
           L SQANELGEFDLILMSDLFY+SEEMPHLAQVLKIISGTD+KIWAASEIRPWTIECITEL
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQHD-ESHEEDNHKILDSFSIFHLRQEERNL 221
           INQGFKITESPVQHD ESHEEDNHK+LDS+SIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLRQEERNL 222

[Close]

BLAST of CsGy3G001930.1 vs. ExPASy TrEMBL
Match: A0A6J1E9L9 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 GN=LOC111431122 PE=4 SV=1)

HSP 1 Score: 350 bits (897), Expect = 3.29e-120
Identity = 174/223 (78.03%), Postives = 195/223 (87.44%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M V+EFQISD N+VIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFDFR K
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
            VIELGAG GLPGL AALLGA RV+LTDVEPLLPGLL NVDANG+ DRVEVRELVWG ++
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180
             S+ANE GEFDLILMSDLFY+SE+M  LA +LKIISG ++KIWAASEIRPWT+EC+TE 
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 INQGFKITESPVQHDE-SHEEDNHKILDSFSIFHLR--QEERN 220
           +N+GFK+ ES VQ DE S E+D+H ILDSFSIFHL+   EERN
Sbjct: 181 MNEGFKVEESQVQLDEPSEEDDDHAILDSFSIFHLQPPDEERN 223

[Close]

BLAST of CsGy3G001930.1 vs. ExPASy TrEMBL
Match: A0A6J1HN15 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=LOC111465095 PE=4 SV=1)

HSP 1 Score: 345 bits (884), Expect = 8.97e-118
Identity = 173/223 (77.58%), Postives = 193/223 (86.55%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M V+EFQISDRN+VIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFD R K
Sbjct: 31  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDIRDK 90

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
            VIELGAG GLPGL AALLGA RV+LTDVEPLLPGLL NVDANG+ DRV+VRELVWG ++
Sbjct: 91  TVIELGAGVGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLVDRVQVRELVWGLDE 150

Query: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180
             S+ANELGEFDLILMSDLFY+SE+M  LA VLKIISG ++KIWAASEIRPWT+EC+ E 
Sbjct: 151 SVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTVECLKEF 210

Query: 181 INQGFKITESPVQHDESHEEDN-HKILDSFSIFHLR--QEERN 220
           +N+GFK+ ES VQ DE  EED+ H ILD FSIFHL+   EERN
Sbjct: 211 MNEGFKVEESQVQLDEPREEDDDHAILDLFSIFHLQPPDEERN 253

[Close]

BLAST of CsGy3G001930.1 vs. TAIR 10
Match: AT5G44170.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.9e-15
Identity = 54/193 (27.98%), Postives = 92/193 (47.67%), Query Frame = 0


Query: 27  GRVLTGSWVWNSAFVLAKWMATQCNLFD-------------FDFRQKNVIELGAGTGLPG 86
           G +  G+ VW  + +L+K+      L               FDFR++  IELG G G+ G
Sbjct: 25  GSMHVGTSVWPCSLILSKFAERWSTLDSSSSTTSPNPYAELFDFRRRRGIELGTGCGVAG 84

Query: 87  LTAALLGANRVLLTDVEPLLPGLLENVDAN--GVGDRVEVRELVWGSNDLPSQANELGEF 146
           +   LLG   ++LTD+ P++P L  N+  N   +G  ++   + W + D  S       F
Sbjct: 85  MAFYLLGLTEIVLTDIAPVMPALKHNLKRNKTALGKSLKTSIVYWNNRDQISALKP--PF 144

Query: 147 DLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIR-PWTIECITELINQGFKITES 201
           DL++ +D+ Y  E +  L   ++++   D  +    +IR P   +   EL +  FKI + 
Sbjct: 145 DLVIAADVVYIEESVGQLVTAMELLVADDGAVLLGYQIRSPEADKLFWELCDIVFKIEKV 204

[Close]

BLAST of CsGy3G001930.1 vs. TAIR 10
Match: AT1G73320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 79.0 bits (193), Expect = 5.5e-15
Identity = 50/145 (34.48%), Postives = 73/145 (50.34%), Query Frame = 0


Query: 30  LTGSWVWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDV 89
           +TGS +W+S  VL K++    +        K ++ELG+G GL G  AALLG N V LTD+
Sbjct: 117 VTGSVMWDSGVVLGKFLEHSVDSKVLSLEGKKIVELGSGCGLVGCIAALLGGNAV-LTDL 176

Query: 90  EPLLPGLLENVDAN----GVGDRVEVRELVWGSNDLPSQANELGEFDLILMSDLFYNSEE 149
              L  L +N+  N           V+ELVWG +  P         D +L SD+ Y+ E 
Sbjct: 177 PDRLRLLKKNIQTNLHRGNTRGSAIVQELVWGDDPDPDLIEPFP--DYVLGSDVIYSEEA 236

Query: 150 MPHLAQVLKIISGTDTKIWAASEIR 171
           + HL + L  +    T I+ + E+R
Sbjct: 237 VHHLVKTLLQLCSDQTTIFLSGELR 258

[Close]

BLAST of CsGy3G001930.1 vs. TAIR 10
Match: AT1G08125.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 6.7e-13
Identity = 54/200 (27.00%), Postives = 92/200 (46.00%), Query Frame = 0


Query: 16  HELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFD---FRQKNVIELGAGTGLP 75
           HELD   D  +  +  G+ VW+++ V AK++        F     + K  IELGAG G+ 
Sbjct: 21  HELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVA 80

Query: 76  GLTAALLGANRVLLTDVEPLLPGLLENVDAN----------GVGDRVEVRELVWGSNDLP 135
           G   A+LG + V+ TD + +LP L  NV+ N               + V EL WG+ D  
Sbjct: 81  GFALAMLGCD-VVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHI 140

Query: 136 SQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTI-ECITELI 195
           +       FD ++ +D+ Y+ + +  L + +  +SG  T +    EIR   + E + ++ 
Sbjct: 141 TAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMW 200

Query: 196 NQGFKITESPVQHDESHEED 202
              F++   P    +   +D
Sbjct: 201 KDNFEVKTIPRSKMDGEYQD 215

[Close]

BLAST of CsGy3G001930.1 vs. TAIR 10
Match: AT1G08125.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 6.7e-13
Identity = 54/200 (27.00%), Postives = 92/200 (46.00%), Query Frame = 0


Query: 16  HELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFD---FRQKNVIELGAGTGLP 75
           HELD   D  +  +  G+ VW+++ V AK++        F     + K  IELGAG G+ 
Sbjct: 28  HELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVA 87

Query: 76  GLTAALLGANRVLLTDVEPLLPGLLENVDAN----------GVGDRVEVRELVWGSNDLP 135
           G   A+LG + V+ TD + +LP L  NV+ N               + V EL WG+ D  
Sbjct: 88  GFALAMLGCD-VVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHI 147

Query: 136 SQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTI-ECITELI 195
           +       FD ++ +D+ Y+ + +  L + +  +SG  T +    EIR   + E + ++ 
Sbjct: 148 TAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMW 207

Query: 196 NQGFKITESPVQHDESHEED 202
              F++   P    +   +D
Sbjct: 208 KDNFEVKTIPRSKMDGEYQD 222

[Close]

BLAST of CsGy3G001930.1 vs. TAIR 10
Match: AT3G50850.1 (Putative methyltransferase family protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.8e-10
Identity = 44/126 (34.92%), Postives = 68/126 (53.97%), Query Frame = 0


Query: 61  NVIELGAGTGLPGL-TAALLGANRVLLTDVEPLLPGLLENVDANG-----VGDRVEVREL 120
           N++ELG+GTG+ G+  AA LGAN V +TD+  ++  L  N DAN       G +V V  L
Sbjct: 91  NIVELGSGTGIVGIAAAATLGAN-VTVTDLPNVIENLKFNADANAQVVAKFGGKVHVASL 150

Query: 121 VWGSNDLPSQANELGE-FDLILMSDLFYNSEEMPHLAQVLK--IISGTDTKIWAASEIRP 178
            WG  D       LG+  DLIL SD+ Y+      L + L+  ++ G+  +++  + ++ 
Sbjct: 151 RWGEID---DVESLGQNVDLILASDVVYHVHLYEPLLKTLRFLLLEGSSERVFLMAHLKR 210

The following BLAST results are available for this feature:

BLAST of CsGy3G001930.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5BLD8	1.4e-18	40.83	Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a P...	[more]
A4FV98	7.0e-15	35.54	EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1	[more]
Q8C436	1.2e-14	36.07	Protein-lysine methyltransferase METTL21D OS=Mus musculus OX=10090 GN=Vcpkmt PE=...	[more]
Q8WXB1	4.5e-14	38.85	Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A ...	[more]
D3YWP0	1.7e-13	35.90	EEF1A lysine methyltransferase 3 OS=Mus musculus OX=10090 GN=eef1akmt3 PE=3 SV=1	[more]

BLAST of CsGy3G001930.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150176.1	2.91e-159	100.00	protein N-lysine methyltransferase METTL21A [Cucumis sativus]	[more]
XP_008448745.1	3.91e-146	92.79	PREDICTED: protein N-lysine methyltransferase METTL21A-like [Cucumis melo] >KAA0...	[more]
XP_022923423.1	6.80e-120	78.03	protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata]	[more]
XP_023553140.1	1.52e-119	78.48	protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo]	[more]
KAG7015681.1	5.57e-119	77.58	EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyros...	[more]

BLAST of CsGy3G001930.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6N4	1.41e-159	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016950 PE=4 SV=1	[more]
A0A5A7UCU5	1.89e-146	92.79	Protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BKF2	1.89e-146	92.79	protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1E9L9	3.29e-120	78.03	protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1HN15	8.97e-118	77.58	protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=...	[more]

BLAST of CsGy3G001930.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44170.1	1.9e-15	27.98	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G73320.1	5.5e-15	34.48	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G08125.1	6.7e-13	27.00	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G08125.2	6.7e-13	27.00	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT3G50850.1	1.8e-10	34.92	Putative methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019410	Lysine methyltransferase	PFAM	PF10294	Methyltransf_16	coord: 31..170 e-value: 9.9E-30 score: 103.5
IPR019410	Lysine methyltransferase	PANTHER	PTHR14614	HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN	coord: 1..218
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..208 e-value: 2.9E-44 score: 153.1
None	No IPR available	PANTHER	PTHR14614:SF123	OSJNBA0070O11.4 PROTEIN	coord: 1..218
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 61..154 e-value: 1.67668E-6 score: 43.5727
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 48..156

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CsGy3G001930	CsGy3G001930	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy3G001930.1.exon1	CsGy3G001930.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.CsGy3G001930.1	cds.CsGy3G001930.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CsGy3G001930.1	CsGy3G001930.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity