CsGy2G004840.1 (mRNA) Cucumber (Gy14) v2.1

Overview


Name	CsGy2G004840.1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	nicotianamine synthase
Location	Gy14Chr2: 3331053 .. 3331706 (+)
Sequence length	654
RNA-Seq Expression	CsGy2G004840.1
Synteny	CsGy2G004840.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGATCCCATCTCATCAAGCTCTGTGGCGAGGCCGAGTCCCTTCTCGAGCATCACTACTCCACCATTTTATCTTCCCACCAAAACCCTCTCGACCATCTCTCCCTCTTCCCTTATTACTCCAACTACCTCAAACTCACTCACTTAGAATTCTCCATTTTGAACCACCACGCCCCCCACAGTACTACTACGCCTCCCTCTAAAGTGGCATTTGTTGGCTCCGGTGCACTTCCCTTCTCGTCTATCATCATGGCCTTGAAGTACTTCCCAAAAACCGAGTTTCACAACTTCGACCTCGACCCGTCTGCCAATGCGAAAGCATCGATGCTGGTAGCAGGCGACGTGGAGCTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGTCGGGTCGGAAGAGTTGAGGGAGTTCGAGGTGGTGTTTCTGGCGGCGTTGGTGGGGATGGAGAGAGAAGAGAAGGGGAAGGTGATCGAGCATTTGAGGAAGAATATGAGTGGTGGGGCGTTGTTGATGTTGAGGAGCGCATATGGTGCACGGGCGTTTGTTTATCCGGTGGTGGAAGCTTGTGATCTTCGGGGGTTCGACATTCTTACGGTGTTTCATCCAACGGATGAAGTTATTAATTCAATTGTGCTGGCACGATTATAA

mRNA sequence

ATGAGATCCCATCTCATCAAGCTCTGTGGCGAGGCCGAGTCCCTTCTCGAGCATCACTACTCCACCATTTTATCTTCCCACCAAAACCCTCTCGACCATCTCTCCCTCTTCCCTTATTACTCCAACTACCTCAAACTCACTCACTTAGAATTCTCCATTTTGAACCACCACGCCCCCCACAGTACTACTACGCCTCCCTCTAAAGTGGCATTTGTTGGCTCCGGTGCACTTCCCTTCTCGTCTATCATCATGGCCTTGAAGTACTTCCCAAAAACCGAGTTTCACAACTTCGACCTCGACCCGTCTGCCAATGCGAAAGCATCGATGCTGGTAGCAGGCGACGTGGAGCTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGTCGGGTCGGAAGAGTTGAGGGAGTTCGAGGTGGTGTTTCTGGCGGCGTTGGTGGGGATGGAGAGAGAAGAGAAGGGGAAGGTGATCGAGCATTTGAGGAAGAATATGAGTGGTGGGGCGTTGTTGATGTTGAGGAGCGCATATGGTGCACGGGCGTTTGTTTATCCGGTGGTGGAAGCTTGTGATCTTCGGGGGTTCGACATTCTTACGGTGTTTCATCCAACGGATGAAGTTATTAATTCAATTGTGCTGGCACGATTATAA

Coding sequence (CDS)

ATGAGATCCCATCTCATCAAGCTCTGTGGCGAGGCCGAGTCCCTTCTCGAGCATCACTACTCCACCATTTTATCTTCCCACCAAAACCCTCTCGACCATCTCTCCCTCTTCCCTTATTACTCCAACTACCTCAAACTCACTCACTTAGAATTCTCCATTTTGAACCACCACGCCCCCCACAGTACTACTACGCCTCCCTCTAAAGTGGCATTTGTTGGCTCCGGTGCACTTCCCTTCTCGTCTATCATCATGGCCTTGAAGTACTTCCCAAAAACCGAGTTTCACAACTTCGACCTCGACCCGTCTGCCAATGCGAAAGCATCGATGCTGGTAGCAGGCGACGTGGAGCTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGTCGGGTCGGAAGAGTTGAGGGAGTTCGAGGTGGTGTTTCTGGCGGCGTTGGTGGGGATGGAGAGAGAAGAGAAGGGGAAGGTGATCGAGCATTTGAGGAAGAATATGAGTGGTGGGGCGTTGTTGATGTTGAGGAGCGCATATGGTGCACGGGCGTTTGTTTATCCGGTGGTGGAAGCTTGTGATCTTCGGGGGTTCGACATTCTTACGGTGTTTCATCCAACGGATGAAGTTATTAATTCAATTGTGCTGGCACGATTATAA

Protein sequence

MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL*

Homology

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BLAST of CsGy2G004840.1 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.0e-70
Identity = 134/217 (61.75%), Postives = 174/217 (80.18%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFSILNHHAP 60
           MRS+LIK+CGEAE  LEHH+S+IL+S + NPL HL+LFPYY+NYLKL+ LEF +L  +  
Sbjct: 62  MRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNL- 121

Query: 61  HSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSR 120
                 P  VAF+GSG LP +S+++A  +   + FHNFD+DPSAN  A+ LV+ D +LS+
Sbjct: 122 --NGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQ 181

Query: 121 RMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYG 180
           RM FHT DIMDV +E L+ F+VVFLAALVGM+++EK KV+EHL K+MS GALLMLRSA+G
Sbjct: 182 RMFFHTVDIMDV-TESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHG 241

Query: 181 ARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLAR 217
            RAF+YP+VE CDL GF++L+V+HPTDEVINSIV++R
Sbjct: 242 PRAFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of CsGy2G004840.1 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 3.4e-70
Identity = 128/217 (58.99%), Postives = 174/217 (80.18%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFSILNHHAP 60
           MRSHLIKLCGEAE  LE H+S IL S + NPL+HL +FPYY+NYLKL  LEF +L+ H  
Sbjct: 62  MRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH-- 121

Query: 61  HSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSR 120
             TT  P+KVAF+GSG +P +SI++A  + P T FHNFD+D  AN  AS LV+ D +LS+
Sbjct: 122 --TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSK 181

Query: 121 RMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYG 180
           RM+FHTTD+++   E L +++VVFLAALVGM++E K K IEHL K+M+ GA++MLRSA+G
Sbjct: 182 RMIFHTTDVLN-AKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHG 241

Query: 181 ARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLAR 217
            RAF+YP+V++CDL+GF++LT++HP+D+V+NS+V+AR
Sbjct: 242 LRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

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BLAST of CsGy2G004840.1 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 2.2e-69
Identity = 129/219 (58.90%), Postives = 173/219 (79.00%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSS---HQNPLDHLSLFPYYSNYLKLTHLEFSILNHH 60
           MR++LIKLCGEAE  LE H+STIL S    QNPLDHL +FPYYSNYLKL  LEF +L+ H
Sbjct: 61  MRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQH 120

Query: 61  APHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVEL 120
           + H     P+K+AFVGSG +P +SI++A  + P T FHNFD+D  AN  AS LV+ D +L
Sbjct: 121 SSH----VPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDL 180

Query: 121 SRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSA 180
           S+RM+FHTTD+++  +E L +++VVFLAALVGM++E K K IEHL K+M+ GA+LMLRSA
Sbjct: 181 SKRMIFHTTDVLN-ATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSA 240

Query: 181 YGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLAR 217
           +  RAF+YP+V++ DL+GF +LT++HPTD+V+NS+V+AR
Sbjct: 241 HALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of CsGy2G004840.1 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 4.8e-69
Identity = 128/216 (59.26%), Postives = 173/216 (80.09%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           +RSHLIKLCG+AE LLE H+S ILSS++NPL HL +FPY+ NY+KL+ LE++IL      
Sbjct: 61  IRSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILT----K 120

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
           +TT  P K+AF+GSG LP +S+++A K+   T FHN+D+D  AN  AS LVA D ++S R
Sbjct: 121 NTTNIPKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSR 180

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           M FHT D+MDV +  L++++VVFLAALVGM++E+K KV++HL K MS GA LMLRSA+GA
Sbjct: 181 MTFHTADVMDV-TCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGA 240

Query: 181 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLAR 217
           RAF+YPV++  DLRGF++L+V+HPTDEVINS+++AR
Sbjct: 241 RAFLYPVLDPRDLRGFEVLSVYHPTDEVINSVIIAR 271

[Close]

BLAST of CsGy2G004840.1 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 9.1e-68
Identity = 128/217 (58.99%), Postives = 175/217 (80.65%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFSILNHHAP 60
           +R +LIK+CG AE  LE+H+S+IL+S+Q NPL HL++FPYY+NYLKL  LEF +L  +  
Sbjct: 61  IRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL- 120

Query: 61  HSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSR 120
                 P  VAF+GSG LP +SI++A  +   T FHNFD+DPSAN+ AS+LV+ D ++S+
Sbjct: 121 --NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQ 180

Query: 121 RMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYG 180
           RM FHT DIMDV +E L+ F+VVFLAALVGM +EEK KVIEHL+K+M+ GA+LMLRSA+G
Sbjct: 181 RMFFHTVDIMDV-TESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHG 240

Query: 181 ARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLAR 217
            RAF+YP+VE CDL+GF++L+++HPTD+VINS+V+++
Sbjct: 241 PRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of CsGy2G004840.1 vs. NCBI nr
Match: XP_004144812.1 (nicotianamine synthase 1 [Cucumis sativus])

HSP 1 Score: 431 bits (1107), Expect = 4.02e-151
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH
Sbjct: 62  MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 121

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
           STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR
Sbjct: 122 STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 181

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA
Sbjct: 182 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 241

Query: 181 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 217
           RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL
Sbjct: 242 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

[Close]

BLAST of CsGy2G004840.1 vs. NCBI nr
Match: KAA0048068.1 (nicotianamine synthase 1-like [Cucumis melo var. makuwa] >TYJ96468.1 nicotianamine synthase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 398 bits (1022), Expect = 7.19e-139
Identity = 204/217 (94.01%), Postives = 208/217 (95.85%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           MRS+LIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH   
Sbjct: 21  MRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHI-- 80

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
             T PPSKVAFVGSGALPFSSIIMALKY PKTEFHNFDLDPSANAKASMLVAGDVELSRR
Sbjct: 81  --TPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELSRR 140

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           MVFHTTDIM+VGSEELREFEVVFLAALVGMEREEK KVIEHLRKNM GGALLMLRSAYGA
Sbjct: 141 MVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAYGA 200

Query: 181 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 217
           RAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 201 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 233

[Close]

BLAST of CsGy2G004840.1 vs. NCBI nr
Match: XP_008453494.1 (PREDICTED: nicotianamine synthase 1-like [Cucumis melo])

HSP 1 Score: 398 bits (1022), Expect = 3.70e-138
Identity = 204/217 (94.01%), Postives = 208/217 (95.85%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           MRS+LIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH   
Sbjct: 67  MRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHI-- 126

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
             T PPSKVAFVGSGALPFSSIIMALKY PKTEFHNFDLDPSANAKASMLVAGDVELSRR
Sbjct: 127 --TPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELSRR 186

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           MVFHTTDIM+VGSEELREFEVVFLAALVGMEREEK KVIEHLRKNM GGALLMLRSAYGA
Sbjct: 187 MVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAYGA 246

Query: 181 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 217
           RAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 247 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

[Close]

BLAST of CsGy2G004840.1 vs. NCBI nr
Match: XP_038891237.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 394 bits (1011), Expect = 1.63e-136
Identity = 198/217 (91.24%), Postives = 208/217 (95.85%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           MRSHLIKLCGEAE+LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH
Sbjct: 64  MRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 123

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
           +   PPSKVAFVGSGALPFSSIIMALKY PKTE HNFD+DPSANAKA+MLVAGD ELS+R
Sbjct: 124 A---PPSKVAFVGSGALPFSSIIMALKYLPKTELHNFDIDPSANAKAAMLVAGDAELSKR 183

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           MVFHTTDIMDV S+ELREFEVVFLAALVGMEREEK KVIEHLRK+M GGALLMLRSAYGA
Sbjct: 184 MVFHTTDIMDVASDELREFEVVFLAALVGMEREEKVKVIEHLRKSMGGGALLMLRSAYGA 243

Query: 181 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 217
           RAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 244 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 277

[Close]

BLAST of CsGy2G004840.1 vs. NCBI nr
Match: KAE8651625.1 (hypothetical protein Csa_021256 [Cucumis sativus])

HSP 1 Score: 391 bits (1004), Expect = 7.83e-136
Identity = 197/198 (99.49%), Postives = 197/198 (99.49%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH
Sbjct: 54  MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 113

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
           STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR
Sbjct: 114 STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 173

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA
Sbjct: 174 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 233

Query: 181 RAFVYPVVEACDLRGFDI 198
           RAFVYPVVEACDLRG DI
Sbjct: 234 RAFVYPVVEACDLRGLDI 251

[Close]

BLAST of CsGy2G004840.1 vs. ExPASy TrEMBL
Match: A0A0A0LJ09 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1)

HSP 1 Score: 431 bits (1107), Expect = 1.94e-151
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH
Sbjct: 62  MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 121

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
           STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR
Sbjct: 122 STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 181

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA
Sbjct: 182 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 241

Query: 181 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 217
           RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL
Sbjct: 242 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

[Close]

BLAST of CsGy2G004840.1 vs. ExPASy TrEMBL
Match: A0A5A7TYM0 (Nicotianamine synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001120 PE=3 SV=1)

HSP 1 Score: 398 bits (1022), Expect = 3.48e-139
Identity = 204/217 (94.01%), Postives = 208/217 (95.85%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           MRS+LIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH   
Sbjct: 21  MRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHI-- 80

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
             T PPSKVAFVGSGALPFSSIIMALKY PKTEFHNFDLDPSANAKASMLVAGDVELSRR
Sbjct: 81  --TPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELSRR 140

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           MVFHTTDIM+VGSEELREFEVVFLAALVGMEREEK KVIEHLRKNM GGALLMLRSAYGA
Sbjct: 141 MVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAYGA 200

Query: 181 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 217
           RAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 201 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 233

[Close]

BLAST of CsGy2G004840.1 vs. ExPASy TrEMBL
Match: A0A1S3BXJ6 (nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1)

HSP 1 Score: 398 bits (1022), Expect = 1.79e-138
Identity = 204/217 (94.01%), Postives = 208/217 (95.85%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           MRS+LIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH   
Sbjct: 67  MRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHI-- 126

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
             T PPSKVAFVGSGALPFSSIIMALKY PKTEFHNFDLDPSANAKASMLVAGDVELSRR
Sbjct: 127 --TPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELSRR 186

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           MVFHTTDIM+VGSEELREFEVVFLAALVGMEREEK KVIEHLRKNM GGALLMLRSAYGA
Sbjct: 187 MVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAYGA 246

Query: 181 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 217
           RAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 247 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

[Close]

BLAST of CsGy2G004840.1 vs. ExPASy TrEMBL
Match: A0A6J1HS54 (nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1)

HSP 1 Score: 353 bits (907), Expect = 6.39e-121
Identity = 180/217 (82.95%), Postives = 196/217 (90.32%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           MRSHLIKLCG+AE LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFSIL  +   
Sbjct: 69  MRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILTRYISP 128

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
           +    PSKVAF+GSGALPFSSIIMALKY P TEFHNFD+D SANAKA+MLVA DV+LSRR
Sbjct: 129 AL---PSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDVDLSRR 188

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           M FHTTDIMDV S+ LREFEVVFLAALVGME+EEK KVIEHLRKNM+ G+LLMLRSAYGA
Sbjct: 189 MTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGA 248

Query: 181 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 217
           RAF+YPVVEA DLRGF+ILT FHPTD+V+NSIVLARL
Sbjct: 249 RAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of CsGy2G004840.1 vs. ExPASy TrEMBL
Match: A0A6J1FHL8 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 SV=1)

HSP 1 Score: 349 bits (896), Expect = 3.02e-119
Identity = 179/217 (82.49%), Postives = 194/217 (89.40%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPH 60
           MRSHLIKLCG+AE LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFSIL  +   
Sbjct: 69  MRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILTRYISS 128

Query: 61  STTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRR 120
           S    PSKVAF+GSGALPFSSIIMALKY P TEFHNFD+D SANAKA+ LVA DV+LSRR
Sbjct: 129 SL---PSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDVDLSRR 188

Query: 121 MVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGA 180
           M FHTTDIMDV S+ L EFEVVFLAALVGME+EEK KVIEHLRKNM+ G+LLMLRSAYGA
Sbjct: 189 MSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGA 248

Query: 181 RAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 217
           RAF+YPVVEA DLRGF+ILT FHPTD+V+NSIVLARL
Sbjct: 249 RAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of CsGy2G004840.1 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 266.9 bits (681), Expect = 1.4e-71
Identity = 134/217 (61.75%), Postives = 174/217 (80.18%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFSILNHHAP 60
           MRS+LIK+CGEAE  LEHH+S+IL+S + NPL HL+LFPYY+NYLKL+ LEF +L  +  
Sbjct: 62  MRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNL- 121

Query: 61  HSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSR 120
                 P  VAF+GSG LP +S+++A  +   + FHNFD+DPSAN  A+ LV+ D +LS+
Sbjct: 122 --NGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQ 181

Query: 121 RMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYG 180
           RM FHT DIMDV +E L+ F+VVFLAALVGM+++EK KV+EHL K+MS GALLMLRSA+G
Sbjct: 182 RMFFHTVDIMDV-TESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHG 241

Query: 181 ARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLAR 217
            RAF+YP+VE CDL GF++L+V+HPTDEVINSIV++R
Sbjct: 242 PRAFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of CsGy2G004840.1 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 266.2 bits (679), Expect = 2.4e-71
Identity = 128/217 (58.99%), Postives = 174/217 (80.18%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFSILNHHAP 60
           MRSHLIKLCGEAE  LE H+S IL S + NPL+HL +FPYY+NYLKL  LEF +L+ H  
Sbjct: 62  MRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH-- 121

Query: 61  HSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSR 120
             TT  P+KVAF+GSG +P +SI++A  + P T FHNFD+D  AN  AS LV+ D +LS+
Sbjct: 122 --TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSK 181

Query: 121 RMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYG 180
           RM+FHTTD+++   E L +++VVFLAALVGM++E K K IEHL K+M+ GA++MLRSA+G
Sbjct: 182 RMIFHTTDVLN-AKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHG 241

Query: 181 ARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLAR 217
            RAF+YP+V++CDL+GF++LT++HP+D+V+NS+V+AR
Sbjct: 242 LRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of CsGy2G004840.1 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 263.5 bits (672), Expect = 1.5e-70
Identity = 129/219 (58.90%), Postives = 173/219 (79.00%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSS---HQNPLDHLSLFPYYSNYLKLTHLEFSILNHH 60
           MR++LIKLCGEAE  LE H+STIL S    QNPLDHL +FPYYSNYLKL  LEF +L+ H
Sbjct: 61  MRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQH 120

Query: 61  APHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVEL 120
           + H     P+K+AFVGSG +P +SI++A  + P T FHNFD+D  AN  AS LV+ D +L
Sbjct: 121 SSH----VPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDL 180

Query: 121 SRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSA 180
           S+RM+FHTTD+++  +E L +++VVFLAALVGM++E K K IEHL K+M+ GA+LMLRSA
Sbjct: 181 SKRMIFHTTDVLN-ATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSA 240

Query: 181 YGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLAR 217
           +  RAF+YP+V++ DL+GF +LT++HPTD+V+NS+V+AR
Sbjct: 241 HALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of CsGy2G004840.1 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 258.1 bits (658), Expect = 6.5e-69
Identity = 128/217 (58.99%), Postives = 175/217 (80.65%), Query Frame = 0


Query: 1   MRSHLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFSILNHHAP 60
           +R +LIK+CG AE  LE+H+S+IL+S+Q NPL HL++FPYY+NYLKL  LEF +L  +  
Sbjct: 61  IRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL- 120

Query: 61  HSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSR 120
                 P  VAF+GSG LP +SI++A  +   T FHNFD+DPSAN+ AS+LV+ D ++S+
Sbjct: 121 --NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQ 180

Query: 121 RMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYG 180
           RM FHT DIMDV +E L+ F+VVFLAALVGM +EEK KVIEHL+K+M+ GA+LMLRSA+G
Sbjct: 181 RMFFHTVDIMDV-TESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHG 240

Query: 181 ARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLAR 217
            RAF+YP+VE CDL+GF++L+++HPTD+VINS+V+++
Sbjct: 241 PRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of CsGy2G004840.1 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 62.4 bits (150), Expect = 5.2e-10
Identity = 28/53 (52.83%), Postives = 41/53 (77.36%), Query Frame = 0


Query: 166 MSGGALLMLRSAYGARAFVYPVVEACDLRGFDILTVFHP--TDEVINSIVLAR 217
           M  GALLMLRSA G R+F+Y  V+ CDL+GF++L ++HP  +D  +NS+++AR
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVAR 53

The following BLAST results are available for this feature:

BLAST of CsGy2G004840.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7X5	2.0e-70	61.75	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9FKT9	3.4e-70	58.99	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]
Q9FF79	2.2e-69	58.90	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9XGI7	4.8e-69	59.26	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
O80483	9.1e-68	58.99	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]

BLAST of CsGy2G004840.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144812.1	4.02e-151	100.00	nicotianamine synthase 1 [Cucumis sativus]	[more]
KAA0048068.1	7.19e-139	94.01	nicotianamine synthase 1-like [Cucumis melo var. makuwa] >TYJ96468.1 nicotianami...	[more]
XP_008453494.1	3.70e-138	94.01	PREDICTED: nicotianamine synthase 1-like [Cucumis melo]	[more]
XP_038891237.1	1.63e-136	91.24	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
KAE8651625.1	7.83e-136	99.49	hypothetical protein Csa_021256 [Cucumis sativus]	[more]

BLAST of CsGy2G004840.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJ09	1.94e-151	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1	[more]
A0A5A7TYM0	3.48e-139	94.01	Nicotianamine synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BXJ6	1.79e-138	94.01	nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1	[more]
A0A6J1HS54	6.39e-121	82.95	nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1	[more]
A0A6J1FHL8	3.02e-119	82.49	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 S...	[more]

BLAST of CsGy2G004840.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	1.4e-71	61.75	nicotianamine synthase 4	[more]
AT5G56080.1	2.4e-71	58.99	nicotianamine synthase 2	[more]
AT5G04950.1	1.5e-70	58.90	nicotianamine synthase 1	[more]
AT1G09240.1	6.5e-69	58.99	nicotianamine synthase 3	[more]
AT4G26483.1	5.2e-10	52.83	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..217 e-value: 4.0E-57 score: 195.4
None	No IPR available	PANTHER	PTHR32266:SF17	SYNTHASE, PUTATIVE-RELATED	coord: 1..216
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 1..216 e-value: 8.9E-89 score: 297.5
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..216
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..217 score: 43.280315

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CsGy2G004840	CsGy2G004840	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy2G004840.1.exon1	CsGy2G004840.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.CsGy2G004840.1	cds.CsGy2G004840.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CsGy2G004840.1	CsGy2G004840.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity