Cp4.1LG20g01960.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g01960.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	protein kinase 2B, chloroplastic-like
Location	Cp4.1LG20: 1128992 .. 1132681 (+)
Sequence length	1388
RNA-Seq Expression	Cp4.1LG20g01960.1
Synteny	Cp4.1LG20g01960.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTAATTGCCTCGAAAAAGCTTGCTCTAAGGTGGATAACTCCCTCAACTCTTTCCCTAATTCTGGTAATTCCTTTCCCCTGTTTCATTTTCATTGTCGATTCCATGAACTCCTTGCTTTTTTCTCCTTTTCTCCTTCTTTCTCGTGGTGGGTTCGTTTCTTTTGGGGTTATTTTGGATAAACTTTCTGGGAATTTTTGTCTGGGTGTGTTGCGAAATGGGTTTGGTTGAATGAAAGGGTTATAGGGATTTTCTTCTCCTTTTTCGACATTTTGTAGCTTGTTCAAATCCGTCTTAAGCTTTAGCTTCCTCTTGCTACCTCCTTTGAACTTTACATTATTGGGTATTGTACTGCGCTTTCGATACTCCCAAATCCATGTGTTTCTTAGCTTTGCAAGTTGAGTGAGTACACTATCCATGGCACTGAGATCTCAGTGCTCTACGTATGAAGCCCATGAAGAATTGTTCTGTATTTAGGGTCAAGTGGATTTCTGTTCAAGTTTAAATTGTTTGTGTAAATTGAACACAATCTATCGTGTCCTGTAAAGTGATAGATGTATGAACTGGCGGGTGAAGATTTCATGTTTCCATTTTGAAAATTACTTTTCGAGTAAATTGGTTATTTTATGAATGTAGTCTAAGGGGACATGGAACAATTACTTTTTTAGTCACTATGAGAGTTCTTATTGATCATGAGCCTGATGTGAAATGCACTAGAGATTATGGTATGATGATAAAAATTGGGTGGAACAAACTGGAGTTGGTTTGAAGATCACAATGAGTGGTAAGGGTGTTGCCCGGGAGGCTGATAGATGTTGGCTGTCGGACGATGGCGATGGTGTTCTGGAAGAGTCTGCTACTATCTCCCTTGTTTATCTGTATTAGATAGACATAGGGTCGGGTTCGATATACAGAGTTCCATTCCAAAACCGACTGACAACGAGAAGAGTAACCCACGTATATCTTATATCGATAGTGAGGTCTCTTCATTTTTGTGATATGAAATCTTCAAGATGTCCCCATAAGATGGTGCCTTTTCGGGTTCACCATTCTTACGTCGGATCCCAATTTTTTGGACCGAATACCCGCTTAGGCTTTATGTGCTCTAATACCTTATTCGATAAACATGAGGTTCCATCTTTAAGTTATAGTAAGCTGTGGAATTTTGGAGAAATCTTATAGAAGTTTTGGCTTGTGGCCAAGTGTAAAAGACAGGATTTGCTCAAGGGATCTATGTGCTCGTTAATGAACCAGCTGAAATATTGTTCTTTGAACTTCTATATCAATTGCTGCCTGTCAGCTAAATAGGATTAGTTTAGTTTCCCGTGCGCATACTGCACATACTTCACTATTCAGTCATTGTAATCGTTGTCGCTCTTATCCCGTGCAGAAGAACCAAAAAAACATAATAGGAAAGGTCATTCATCTGCTTCTTTGAGAGCTCCATCTCTTGGCGGGAGTACGCCATCTAGAGGTCTTCAAGCTCATAGATCCGAAGGTGAAATATTGCTGTCTCCAAATCTGAAAGCCTTCACATACAGTGAGCTTAGGAATGCTACTAAAAACTTCCGCCCTGACAGTCTAATTGGAGAAGGAGGGTTTGGTCATGTTTACAAAGGATGGATTGATGAACACACACTAGAAGCTGTGAGACCTGGAGCTGGAATGGTTGTTGCAGTGAAGAAGCTTAAACCTGAAGGCTTTCAAGGACACAAGGAATGGCTGGTACTTTGCTCTTTATTTTTACACGTCCTCGTAATCTTTCTGGCTCGATTTGCTTTATAAATCAGGTTGCTATTGATTGCAGTCCGAAGTTAACTACCTCGGTCAGCTTCATCATCCGAATCTGGTTAAACTAATCGGGTTCTGCCTTGATGGGGACAGCCGACTTTTAGTCTACGAGTATATGTCCAAAGGCAGCCTCGAAAATCATCTATTTAGAAGTAAGTAATGTTTTGGCCAACTCTAACTGTAACCCTGCTGCAATCATTAACCTTTTTGTGGCAATGTTTTTACTCATTAAGTAGGTTCTAAACCTCTGTTCTTGAATGTGCAATGGCATTGGCAGGGGGTGCGCGACCACTTTCATGGGCAATAAGGATCAAAGTTGCTATCGGTGCTGCTCGAGGGCTTTCCTTTCTGCATGACTCGGAAGAACCAGTTATCTATCGCGATTTCAAAGCATCGAACATCCTTTTAGATTCAGTATGTCATTTAAGTGCATCATGGCAAAGAGTTCTGTTACACCTTTATTTCGTTGCCATTCCCTCTAAGTTCATCTGTCTGTACACAGGAATTCAATGCAAAACTTTCAGATTTTGGTTTGGCAAAAGCAGGCCCAACTGGTGATCGGACTCATGTGTCCACTCAAGTTATGGGTACTCAAGGCTATGCCGCTCCAGAATATATTGCCACTGGTTTGTTCTTCATCTCAGTCTCATGATATGTGGAATTACTGTAGGATATGTGCATTCTAGATCATGATAGATACGTTGAGCTACCAGCTTGGTAATTCAACGTTTTTTCTCCTCCATCTTCTGTTTCGATCTATCGTTAAATTTCGAATATGCTCGTTTGTCTGGTAGATGAAATCTAATTATAATAAATCTTCTCAAGGGAAATCTTTGATGCCTTTTCTCTTGAAGTGAATTGATTGGCAAATCCTTGAGAAGTTTTGCAGCTACTAGAATAAGAATAGCAACTACTGAGCATGAAATCTGTCTACACACTTCTGATGGATAAATCTCTATGCTTCCAATTGCAGGTAGGCTAACAGCTAAGTGTGATGTGTATAGCTTTGGGGTTGTACTGCTGGAGCTTCTTTCGGGACGTCGTGCTGTTGATAAAACTAAAGTTGGTATCGAACAGAATCTTGTGGAATGGGCGAAACCGTACTTGGCTGATAAACGCAAGTTATTTCGGATAATGGACACGAAGTTAGAGGGCCAATACCCTCAAAAAGCTGCGTACATGACTACAGTACTTGCATTGCAATGCATTCGAGAGGCAAAATTACGACCACAGATGTCCGAAGTTCTATCTTCTCTTGAAGAGCTCCCCCTGGTTAAGCATGTCGATAACCAAAGATTAAACAGTCCGGGGCCGAAGTCCCCGCTGAGACATACACCAAAAGCGAGCTCTCCATTACCATCCAAATGAAAATTGTAGGGACCTTTGAGATGAGATGGATCACGTTTTTATTAATGACCCAAGTAAATAAATATTTTTTTGACTTAGGCACGTTCGGTTGGTAGACTGGAGCAGTCGAAATTCTCAACTCGTCTCCGACTATAACTTGGATAGAGCAAAGAGGCCAATATTCTGACCGAAGAGAACGAGCGCGATCAATGTTTCTATTCTGCAAACAACAGCAAATCAGTTAAATTTGATTGGTTTTTTGAGATGAAATGGAGGTGGGAAAAATGTTGAGCAGCTGCTATAATAAATTTTGCAGAGATAAGATAACACTAAACCTTTACATAGAAGGGATGGAAATAATGAGTAGCAAACAGTGATTAAGAGAGTTGCTTTAAAAGTTGCCATTTTTTCCAGACATTTATGCTTTGGCAATCAAACCTTAAACATATCATTGGCAGCATCATTTCTTATGTTGCAGACAACCCAACCAACCAAAGTTTTGGTTTTTATTGTCTTTGTTCATTGACCAATCACGGACAGAGAGAAGCAAA

mRNA sequence

ATGGAAGAACCAAAAAAACATAATAGGAAAGGTCATTCATCTGCTTCTTTGAGAGCTCCATCTCTTGGCGGGAGTACGCCATCTAGAGGTCTTCAAGCTCATAGATCCGAAGGTGAAATATTGCTGTCTCCAAATCTGAAAGCCTTCACATACAGTGAGCTTAGGAATGCTACTAAAAACTTCCGCCCTGACAGTCTAATTGGAGAAGGAGGGTTTGGTCATGTTTACAAAGGATGGATTGATGAACACACACTAGAAGCTGTGAGACCTGGAGCTGGAATGGTTGTTGCAGTGAAGAAGCTTAAACCTGAAGGCTTTCAAGGACACAAGGAATGGCTGGAATTCAATGCAAAACTTTCAGATTTTGGTTTGGCAAAAGCAGGCCCAACTGGTGATCGGACTCATGTGTCCACTCAAGTTATGGGTACTCAAGGCTATGCCGCTCCAGAATATATTGCCACTGGTAGGCTAACAGCTAAGTGTGATGTGTATAGCTTTGGGGTTGTACTGCTGGAGCTTCTTTCGGGACGTCGTGCTGTTGATAAAACTAAAGTTGGTATCGAACAGAATCTTGTGGAATGGGCGAAACCGTACTTGGCTGATAAACGCAAGTTATTTCGGATAATGGACACGAAGTTAGAGGGCCAATACCCTCAAAAAGCTGCGTACATGACTACAGTACTTGCATTGCAATGCATTCGAGAGGCAAAATTACGACCACAGATGTCCGAAGTTCTATCTTCTCTTGAAGAGCTCCCCCTGGTTAAGCATGTCGATAACCAAAGATTAAACAGTCCGGGGCCGAAGTCCCCGCTGAGACATACACCAAAAGCGAGCTCTCCATTACCATCCAAATGAAAATTGTAGGGACCTTTGAGATGAGATGGATCACGTTTTTATTAATGACCCAAGTAAATAAATATTTTTTTGACTTAGGCACGTTCGGTTGGTAGACTGGAGCAGTCGAAATTCTCAACTCGTCTCCGACTATAACTTGGATAGAGCAAAGAGGCCAATATTCTGACCGAAGAGAACGAGCGCGATCAATGTTTCTATTCTGCAAACAACAGCAAATCAGTTAAATTTGATTGGTTTTTTGAGATGAAATGGAGGTGGGAAAAATGTTGAGCAGCTGCTATAATAAATTTTGCAGAGATAAGATAACACTAAACCTTTACATAGAAGGGATGGAAATAATGAGTAGCAAACAGTGATTAAGAGAGTTGCTTTAAAAGTTGCCATTTTTTCCAGACATTTATGCTTTGGCAATCAAACCTTAAACATATCATTGGCAGCATCATTTCTTATGTTGCAGACAACCCAACCAACCAAAGTTTTGGTTTTTATTGTCTTTGTTCATTGACCAATCACGGACAGAGAGAAGCAAA

Coding sequence (CDS)

ATGGAAGAACCAAAAAAACATAATAGGAAAGGTCATTCATCTGCTTCTTTGAGAGCTCCATCTCTTGGCGGGAGTACGCCATCTAGAGGTCTTCAAGCTCATAGATCCGAAGGTGAAATATTGCTGTCTCCAAATCTGAAAGCCTTCACATACAGTGAGCTTAGGAATGCTACTAAAAACTTCCGCCCTGACAGTCTAATTGGAGAAGGAGGGTTTGGTCATGTTTACAAAGGATGGATTGATGAACACACACTAGAAGCTGTGAGACCTGGAGCTGGAATGGTTGTTGCAGTGAAGAAGCTTAAACCTGAAGGCTTTCAAGGACACAAGGAATGGCTGGAATTCAATGCAAAACTTTCAGATTTTGGTTTGGCAAAAGCAGGCCCAACTGGTGATCGGACTCATGTGTCCACTCAAGTTATGGGTACTCAAGGCTATGCCGCTCCAGAATATATTGCCACTGGTAGGCTAACAGCTAAGTGTGATGTGTATAGCTTTGGGGTTGTACTGCTGGAGCTTCTTTCGGGACGTCGTGCTGTTGATAAAACTAAAGTTGGTATCGAACAGAATCTTGTGGAATGGGCGAAACCGTACTTGGCTGATAAACGCAAGTTATTTCGGATAATGGACACGAAGTTAGAGGGCCAATACCCTCAAAAAGCTGCGTACATGACTACAGTACTTGCATTGCAATGCATTCGAGAGGCAAAATTACGACCACAGATGTCCGAAGTTCTATCTTCTCTTGAAGAGCTCCCCCTGGTTAAGCATGTCGATAACCAAAGATTAAACAGTCCGGGGCCGAAGTCCCCGCTGAGACATACACCAAAAGCGAGCTCTCCATTACCATCCAAATGA

Protein sequence

MEEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNFRPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMDTKLEGQYPQKAAYMTTVLALQCIREAKLRPQMSEVLSSLEELPLVKHVDNQRLNSPGPKSPLRHTPKASSPLPSK

Homology

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BLAST of Cp4.1LG20g01960.1 vs. ExPASy Swiss-Prot
Match: Q5PP29 (Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN=PBL4 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.6e-88
Identity = 179/333 (53.75%), Postives = 207/333 (62.16%), Query Frame = 0


Query: 13  SSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNFRPDSLIGEGGF 72
           SS ++++ S    T    LQ  RSEGE+L SP LKAFT++EL+ AT+NFRPDS+IGEGGF
Sbjct: 35  SSLTIQSSSYNDDTSVASLQTPRSEGELLASPTLKAFTFNELKTATRNFRPDSVIGEGGF 94

Query: 73  GHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL------------------- 132
           G+VYKGWIDE TL   +PG+GMVVAVKKLK EGFQGH++WL                   
Sbjct: 95  GYVYKGWIDERTLSPSKPGSGMVVAVKKLKEEGFQGHRQWLAEVDCLGRLHHMNLVKLIG 154

Query: 133 ------------------------------------------------------------ 192
                                                                       
Sbjct: 155 YCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLAFLHEAQVIYR 214

Query: 193 -----------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCD 252
                      EFNAKLSDFGLAK GPTGDRTHVSTQVMGTQGYAAPEY+ATGR+TAK D
Sbjct: 215 DFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYVATGRITAKSD 274

Query: 253 VYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMDTKLEGQYPQKAA 255
           VYSFGVVLLELLSGR  VDKTKVG+E+NLV+WA PYL DKRK+FRIMDTKL GQYP K A
Sbjct: 275 VYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTKLGGQYPHKGA 334

[Close]

BLAST of Cp4.1LG20g01960.1 vs. ExPASy Swiss-Prot
Match: O49840 (Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN=PBL3 PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 1.4e-87
Identity = 177/328 (53.96%), Postives = 205/328 (62.50%), Query Frame = 0


Query: 21  SLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNFRPDSLIGEGGFGHVYKGWI 80
           S+   +    L   R+EGEIL SPNLKAFT++EL+NAT+NFRPDSL+GEGGFG+V+KGWI
Sbjct: 43  SINSYSSVESLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWI 102

Query: 81  DEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL--------------------------- 140
           D  TL A +PG+G+VVAVKKLK EG+QGHKEWL                           
Sbjct: 103 DGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENR 162

Query: 141 ------------------------------------------------------------ 200
                                                                       
Sbjct: 163 LLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANI 222

Query: 201 ----EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCDVYSFGVV 257
               EFN+KLSDFGLAKAGPTGD+THVSTQVMGT GYAAPEY+ATGRLTAK DVYSFGVV
Sbjct: 223 LLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVV 282

[Close]

BLAST of Cp4.1LG20g01960.1 vs. ExPASy Swiss-Prot
Match: O49839 (Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN=PBL2 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 1.7e-82
Identity = 187/383 (48.83%), Postives = 220/383 (57.44%), Query Frame = 0


Query: 11  GHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNFRPDSLIGEG 70
           G S+ S    S  G  P+      R+EGEIL SPNLKAFT++EL+NATKNFR D+L+GEG
Sbjct: 41  GLSTTSYSTDSSFGPLPTL-----RTEGEILSSPNLKAFTFNELKNATKNFRQDNLLGEG 100

Query: 71  GFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL----------------- 130
           GFG V+KGWID+ +L A RPG+G+VVAVK+LKPEGFQGHKEWL                 
Sbjct: 101 GFGCVFKGWIDQTSLTASRPGSGIVVAVKQLKPEGFQGHKEWLTEVNYLGQLSHPNLVLL 160

Query: 131 ------------------------------------------------------------ 190
                                                                       
Sbjct: 161 VGYCAEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAVGAAKGLTFLHEAKSQV 220

Query: 191 --------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTA 250
                         +FNAKLSDFGLAKAGPTGD THVST+V+GT GYAAPEY+ATGRLTA
Sbjct: 221 IYRDFKAANILLDADFNAKLSDFGLAKAGPTGDNTHVSTKVIGTHGYAAPEYVATGRLTA 280

Query: 251 KCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMDTKLEGQYPQ 285
           K DVYSFGVVLLEL+SGRRA+D +  G E +LV+WA PYL DKRKLFRIMDTKL GQYPQ
Sbjct: 281 KSDVYSFGVVLLELISGRRAMDNSNGGNEYSLVDWATPYLGDKRKLFRIMDTKLGGQYPQ 340

[Close]

BLAST of Cp4.1LG20g01960.1 vs. ExPASy Swiss-Prot
Match: Q5XF79 (Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 GN=PBL18 PE=2 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.4e-79
Identity = 171/341 (50.15%), Postives = 204/341 (59.82%), Query Frame = 0


Query: 5   KKHNRKGHSSASLRAPSLGG---STPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 64
           +K N+    S SL  PS      +T S      RSEGE+L SP LKAFT++EL+ AT+NF
Sbjct: 26  RKPNQSSRLS-SLTIPSYSNNSFTTSSWSNLTPRSEGELLPSPTLKAFTFNELKTATRNF 85

Query: 65  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 124
           +P+S+IGEGGFG VYKGWI E +L   +PG+GMVVAVKKLK EGFQGHKEWL        
Sbjct: 86  KPNSMIGEGGFGCVYKGWIGERSLSPSKPGSGMVVAVKKLKSEGFQGHKEWLTEVHYLGR 145

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 146 LHHMNLVKLIGYCLEGEKRLLVYEYMPKGSLENHLFRRGAEPIPWKTRMKVAFSAARGLS 205

Query: 185 ---------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYI 244
                                +FNAKLSDFGLAKAGPTGDRTHV+TQV+GTQGYAAPEYI
Sbjct: 206 FLHEAKVIYRDFKASNILLDVDFNAKLSDFGLAKAGPTGDRTHVTTQVIGTQGYAAPEYI 265

Query: 245 ATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMDTK 253
           ATGRLT+K DVYSFGVVLLELLSGR  +DK+KVG+E+NLV+WA PYL D+RK+FRIMDTK
Sbjct: 266 ATGRLTSKSDVYSFGVVLLELLSGRPTLDKSKVGVERNLVDWAIPYLVDRRKVFRIMDTK 325

[Close]

BLAST of Cp4.1LG20g01960.1 vs. ExPASy Swiss-Prot
Match: Q06548 (Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN=PBL9 PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.1e-72
Identity = 152/327 (46.48%), Postives = 191/327 (58.41%), Query Frame = 0


Query: 21  SLGGSTPSRGLQ-AHRSEGEILLSPNLKAFTYSELRNATKNFRPDSLIGEGGFGHVYKGW 80
           SLG    S  ++ + R+EGEIL SPNLK+F+++EL++AT+NFRPDS++GEGGFG V+KGW
Sbjct: 27  SLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGW 86

Query: 81  IDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------------------------- 140
           IDE +L A RPG G+V+AVKKL  +G+QGH+EWL                          
Sbjct: 87  IDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEH 146

Query: 141 ------------------------------------------------------------ 200
                                                                       
Sbjct: 147 RLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKT 206

Query: 201 -------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCDVYSF 253
                  E+NAKLSDFGLAK GP GD++HVST+VMGT GYAAPEY+ATG LT K DVYSF
Sbjct: 207 SNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSF 266

[Close]

BLAST of Cp4.1LG20g01960.1 vs. NCBI nr
Match: KAG7019864.1 (putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 525 bits (1353), Expect = 7.21e-186
Identity = 284/375 (75.73%), Postives = 284/375 (75.73%), Query Frame = 0


Query: 2   EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 61
           EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF
Sbjct: 3   EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 62

Query: 62  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 121
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL        
Sbjct: 63  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 122

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 123 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 182

Query: 182 -----------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 241
                                  EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 183 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 242

Query: 242 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 285
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD
Sbjct: 243 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 302

[Close]

BLAST of Cp4.1LG20g01960.1 vs. NCBI nr
Match: XP_022923884.1 (probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita moschata] >XP_023519798.1 probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519799.1 probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525 bits (1353), Expect = 1.48e-185
Identity = 284/375 (75.73%), Postives = 284/375 (75.73%), Query Frame = 0


Query: 2   EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 61
           EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF
Sbjct: 23  EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 82

Query: 62  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 121
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL        
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 202

Query: 182 -----------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 241
                                  EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 242 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 285
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 322

[Close]

BLAST of Cp4.1LG20g01960.1 vs. NCBI nr
Match: XP_023001520.1 (probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 524 bits (1350), Expect = 4.23e-185
Identity = 283/375 (75.47%), Postives = 284/375 (75.73%), Query Frame = 0


Query: 2   EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 61
           EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF
Sbjct: 23  EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 82

Query: 62  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 121
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL        
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 202

Query: 182 -----------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 241
                                  EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 242 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 285
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWA+PYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWARPYLADKRKLFRIMD 322

[Close]

BLAST of Cp4.1LG20g01960.1 vs. NCBI nr
Match: KAG6584267.1 (putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 523 bits (1348), Expect = 8.53e-185
Identity = 283/375 (75.47%), Postives = 284/375 (75.73%), Query Frame = 0


Query: 2   EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 61
           EEPKKH+RKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF
Sbjct: 23  EEPKKHDRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 82

Query: 62  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 121
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL        
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 202

Query: 182 -----------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 241
                                  EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 242 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 285
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 322

[Close]

BLAST of Cp4.1LG20g01960.1 vs. NCBI nr
Match: XP_008437450.1 (PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo])

HSP 1 Score: 495 bits (1275), Expect = 1.51e-173
Identity = 269/384 (70.05%), Postives = 278/384 (72.40%), Query Frame = 0


Query: 2   EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 61
           E+PK H+RKGHSSASLRAPSLGG+TPSRGLQ HR+EGEILLSPNLKAFTYSEL+NATKNF
Sbjct: 23  EDPKAHDRKGHSSASLRAPSLGGNTPSRGLQVHRTEGEILLSPNLKAFTYSELKNATKNF 82

Query: 62  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 121
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL        
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLT 202

Query: 182 -----------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 241
                                  EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 242 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 285
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVG+EQNLVEWA+PYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMD 322

[Close]

BLAST of Cp4.1LG20g01960.1 vs. ExPASy TrEMBL
Match: A0A6J1E7M0 (probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431470 PE=3 SV=1)

HSP 1 Score: 525 bits (1353), Expect = 7.16e-186
Identity = 284/375 (75.73%), Postives = 284/375 (75.73%), Query Frame = 0


Query: 2   EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 61
           EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF
Sbjct: 23  EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 82

Query: 62  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 121
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL        
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 202

Query: 182 -----------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 241
                                  EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 242 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 285
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 322

[Close]

BLAST of Cp4.1LG20g01960.1 vs. ExPASy TrEMBL
Match: A0A6J1KLE7 (probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495635 PE=3 SV=1)

HSP 1 Score: 524 bits (1350), Expect = 2.05e-185
Identity = 283/375 (75.47%), Postives = 284/375 (75.73%), Query Frame = 0


Query: 2   EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 61
           EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF
Sbjct: 23  EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 82

Query: 62  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 121
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL        
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 202

Query: 182 -----------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 241
                                  EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 242 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 285
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWA+PYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWARPYLADKRKLFRIMD 322

[Close]

BLAST of Cp4.1LG20g01960.1 vs. ExPASy TrEMBL
Match: A0A1S3AU61 (protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103482862 PE=3 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 7.30e-174
Identity = 269/384 (70.05%), Postives = 278/384 (72.40%), Query Frame = 0


Query: 2   EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 61
           E+PK H+RKGHSSASLRAPSLGG+TPSRGLQ HR+EGEILLSPNLKAFTYSEL+NATKNF
Sbjct: 23  EDPKAHDRKGHSSASLRAPSLGGNTPSRGLQVHRTEGEILLSPNLKAFTYSELKNATKNF 82

Query: 62  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 121
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL        
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLT 202

Query: 182 -----------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 241
                                  EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 242 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 285
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVG+EQNLVEWA+PYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMD 322

[Close]

BLAST of Cp4.1LG20g01960.1 vs. ExPASy TrEMBL
Match: A0A6J1C6M0 (probable serine/threonine-protein kinase PBL3 OS=Momordica charantia OX=3673 GN=LOC111008885 PE=3 SV=1)

HSP 1 Score: 454 bits (1169), Expect = 1.10e-157
Identity = 246/367 (67.03%), Postives = 261/367 (71.12%), Query Frame = 0


Query: 3   EPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNFR 62
           EPKK   K HSSA+L+APSLG + PSR LQAHRSEGEILLSPNLKAFT+SEL+NATKNFR
Sbjct: 22  EPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFR 81

Query: 63  PDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL--------- 122
           PDSLIGEGGFG+VYKGWIDEHTLEAVRPGAGMV+AVKKLKPEGFQGHKEWL         
Sbjct: 82  PDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQL 141

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 142 HHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF 201

Query: 183 ----------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 242
                                 EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY
Sbjct: 202 LHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 261

Query: 243 IATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMDT 274
           IATGRLTA+CDVYSFGVVLLELLSGRRAVDKTKVG+EQ+LV+WAKPYLADKRKLFRIMDT
Sbjct: 262 IATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDT 321

[Close]

BLAST of Cp4.1LG20g01960.1 vs. ExPASy TrEMBL
Match: A0A6J1GSG8 (probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=LOC111457081 PE=3 SV=1)

HSP 1 Score: 433 bits (1114), Expect = 2.12e-149
Identity = 237/383 (61.88%), Postives = 262/383 (68.41%), Query Frame = 0


Query: 2   EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 61
           E P   +RKGHS+A+LRAP+LGG+TPSRGLQ  R+EGEILLSPNLKAFTYSEL+NATK+F
Sbjct: 22  ETPNNQDRKGHSTAALRAPALGGNTPSRGLQPKRTEGEILLSPNLKAFTYSELKNATKSF 81

Query: 62  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 121
           RPD+LIGEGGFGHVYKGW+DE TLE+V+PG GMVVAVKKLK EGFQGHKEWL        
Sbjct: 82  RPDTLIGEGGFGHVYKGWLDEQTLESVKPGTGMVVAVKKLKSEGFQGHKEWLSEVTHLSQ 141

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 142 LHHPHLVKLIGFCLDGESRLLVYEYMSKGSLENHLFRMGARPLSWARRIKVAIGAARGLS 201

Query: 182 -----------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 241
                                  EFNAKLSDFGLAKAGPTGDR+HVST+V+GTQGYAAPE
Sbjct: 202 FLHNSAEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRSHVSTRVIGTQGYAAPE 261

Query: 242 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 284
           YIATG LTAKCDVYSFGVVLLELLSGRR VDKTKVG+EQ+LV+WA+PYLADKRKLFRIMD
Sbjct: 262 YIATGMLTAKCDVYSFGVVLLELLSGRRVVDKTKVGVEQHLVDWARPYLADKRKLFRIMD 321

[Close]

BLAST of Cp4.1LG20g01960.1 vs. TAIR 10
Match: AT1G26970.1 (Protein kinase superfamily protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.1e-89
Identity = 179/333 (53.75%), Postives = 207/333 (62.16%), Query Frame = 0


Query: 13  SSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNFRPDSLIGEGGF 72
           SS ++++ S    T    LQ  RSEGE+L SP LKAFT++EL+ AT+NFRPDS+IGEGGF
Sbjct: 35  SSLTIQSSSYNDDTSVASLQTPRSEGELLASPTLKAFTFNELKTATRNFRPDSVIGEGGF 94

Query: 73  GHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL------------------- 132
           G+VYKGWIDE TL   +PG+GMVVAVKKLK EGFQGH++WL                   
Sbjct: 95  GYVYKGWIDERTLSPSKPGSGMVVAVKKLKEEGFQGHRQWLAEVDCLGRLHHMNLVKLIG 154

Query: 133 ------------------------------------------------------------ 192
                                                                       
Sbjct: 155 YCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLAFLHEAQVIYR 214

Query: 193 -----------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCD 252
                      EFNAKLSDFGLAK GPTGDRTHVSTQVMGTQGYAAPEY+ATGR+TAK D
Sbjct: 215 DFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYVATGRITAKSD 274

Query: 253 VYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMDTKLEGQYPQKAA 255
           VYSFGVVLLELLSGR  VDKTKVG+E+NLV+WA PYL DKRK+FRIMDTKL GQYP K A
Sbjct: 275 VYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTKLGGQYPHKGA 334

[Close]

BLAST of Cp4.1LG20g01960.1 vs. TAIR 10
Match: AT2G02800.1 (protein kinase 2B )

HSP 1 Score: 324.3 bits (830), Expect = 9.6e-89
Identity = 177/328 (53.96%), Postives = 205/328 (62.50%), Query Frame = 0


Query: 21  SLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNFRPDSLIGEGGFGHVYKGWI 80
           S+   +    L   R+EGEIL SPNLKAFT++EL+NAT+NFRPDSL+GEGGFG+V+KGWI
Sbjct: 43  SINSYSSVESLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWI 102

Query: 81  DEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL--------------------------- 140
           D  TL A +PG+G+VVAVKKLK EG+QGHKEWL                           
Sbjct: 103 DGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENR 162

Query: 141 ------------------------------------------------------------ 200
                                                                       
Sbjct: 163 LLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANI 222

Query: 201 ----EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCDVYSFGVV 257
               EFN+KLSDFGLAKAGPTGD+THVSTQVMGT GYAAPEY+ATGRLTAK DVYSFGVV
Sbjct: 223 LLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVV 282

[Close]

BLAST of Cp4.1LG20g01960.1 vs. TAIR 10
Match: AT2G02800.2 (protein kinase 2B )

HSP 1 Score: 324.3 bits (830), Expect = 9.6e-89
Identity = 177/328 (53.96%), Postives = 205/328 (62.50%), Query Frame = 0


Query: 21  SLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNFRPDSLIGEGGFGHVYKGWI 80
           S+   +    L   R+EGEIL SPNLKAFT++EL+NAT+NFRPDSL+GEGGFG+V+KGWI
Sbjct: 43  SINSYSSVESLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWI 102

Query: 81  DEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL--------------------------- 140
           D  TL A +PG+G+VVAVKKLK EG+QGHKEWL                           
Sbjct: 103 DGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENR 162

Query: 141 ------------------------------------------------------------ 200
                                                                       
Sbjct: 163 LLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANI 222

Query: 201 ----EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCDVYSFGVV 257
               EFN+KLSDFGLAKAGPTGD+THVSTQVMGT GYAAPEY+ATGRLTAK DVYSFGVV
Sbjct: 223 LLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVV 282

[Close]

BLAST of Cp4.1LG20g01960.1 vs. TAIR 10
Match: AT1G14370.1 (protein kinase 2A )

HSP 1 Score: 307.4 bits (786), Expect = 1.2e-83
Identity = 187/383 (48.83%), Postives = 220/383 (57.44%), Query Frame = 0


Query: 11  GHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNFRPDSLIGEG 70
           G S+ S    S  G  P+      R+EGEIL SPNLKAFT++EL+NATKNFR D+L+GEG
Sbjct: 41  GLSTTSYSTDSSFGPLPTL-----RTEGEILSSPNLKAFTFNELKNATKNFRQDNLLGEG 100

Query: 71  GFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL----------------- 130
           GFG V+KGWID+ +L A RPG+G+VVAVK+LKPEGFQGHKEWL                 
Sbjct: 101 GFGCVFKGWIDQTSLTASRPGSGIVVAVKQLKPEGFQGHKEWLTEVNYLGQLSHPNLVLL 160

Query: 131 ------------------------------------------------------------ 190
                                                                       
Sbjct: 161 VGYCAEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAVGAAKGLTFLHEAKSQV 220

Query: 191 --------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTA 250
                         +FNAKLSDFGLAKAGPTGD THVST+V+GT GYAAPEY+ATGRLTA
Sbjct: 221 IYRDFKAANILLDADFNAKLSDFGLAKAGPTGDNTHVSTKVIGTHGYAAPEYVATGRLTA 280

Query: 251 KCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMDTKLEGQYPQ 285
           K DVYSFGVVLLEL+SGRRA+D +  G E +LV+WA PYL DKRKLFRIMDTKL GQYPQ
Sbjct: 281 KSDVYSFGVVLLELISGRRAMDNSNGGNEYSLVDWATPYLGDKRKLFRIMDTKLGGQYPQ 340

[Close]

BLAST of Cp4.1LG20g01960.1 vs. TAIR 10
Match: AT1G69790.1 (Protein kinase superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 9.7e-81
Identity = 171/341 (50.15%), Postives = 204/341 (59.82%), Query Frame = 0


Query: 5   KKHNRKGHSSASLRAPSLGG---STPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 64
           +K N+    S SL  PS      +T S      RSEGE+L SP LKAFT++EL+ AT+NF
Sbjct: 26  RKPNQSSRLS-SLTIPSYSNNSFTTSSWSNLTPRSEGELLPSPTLKAFTFNELKTATRNF 85

Query: 65  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWL-------- 124
           +P+S+IGEGGFG VYKGWI E +L   +PG+GMVVAVKKLK EGFQGHKEWL        
Sbjct: 86  KPNSMIGEGGFGCVYKGWIGERSLSPSKPGSGMVVAVKKLKSEGFQGHKEWLTEVHYLGR 145

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 146 LHHMNLVKLIGYCLEGEKRLLVYEYMPKGSLENHLFRRGAEPIPWKTRMKVAFSAARGLS 205

Query: 185 ---------------------EFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYI 244
                                +FNAKLSDFGLAKAGPTGDRTHV+TQV+GTQGYAAPEYI
Sbjct: 206 FLHEAKVIYRDFKASNILLDVDFNAKLSDFGLAKAGPTGDRTHVTTQVIGTQGYAAPEYI 265

Query: 245 ATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMDTK 253
           ATGRLT+K DVYSFGVVLLELLSGR  +DK+KVG+E+NLV+WA PYL D+RK+FRIMDTK
Sbjct: 266 ATGRLTSKSDVYSFGVVLLELLSGRPTLDKSKVGVERNLVDWAIPYLVDRRKVFRIMDTK 325

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g01960.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5PP29	1.6e-88	53.75	Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49840	1.4e-87	53.96	Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49839	1.7e-82	48.83	Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q5XF79	1.4e-79	50.15	Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q06548	2.1e-72	46.48	Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of Cp4.1LG20g01960.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019864.1	7.21e-186	75.73	putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma s...	[more]
XP_022923884.1	1.48e-185	75.73	probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita moschata] >X...	[more]
XP_023001520.1	4.23e-185	75.47	probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita maxima]	[more]
KAG6584267.1	8.53e-185	75.47	putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma s...	[more]
XP_008437450.1	1.51e-173	70.05	PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo]	[more]

BLAST of Cp4.1LG20g01960.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E7M0	7.16e-186	75.73	probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita moschata O...	[more]
A0A6J1KLE7	2.05e-185	75.47	probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita maxima OX=...	[more]
A0A1S3AU61	7.30e-174	70.05	protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103482862 PE...	[more]
A0A6J1C6M0	1.10e-157	67.03	probable serine/threonine-protein kinase PBL3 OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1GSG8	2.12e-149	61.88	probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of Cp4.1LG20g01960.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26970.1	1.1e-89	53.75	Protein kinase superfamily protein	[more]
AT2G02800.1	9.6e-89	53.96	protein kinase 2B	[more]
AT2G02800.2	9.6e-89	53.96	protein kinase 2B	[more]
AT1G14370.1	1.2e-83	48.83	protein kinase 2A	[more]
AT1G69790.1	9.7e-81	50.15	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 118..266 e-value: 1.1E-24 score: 88.9
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 42..117 e-value: 3.3E-15 score: 58.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 14..32
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 260..285
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..32
None	No IPR available	PANTHER	PTHR45621:SF109	PROTEIN KINASE 2B, CHLOROPLASTIC	coord: 14..113
None	No IPR available	PANTHER	PTHR45621:SF109	PROTEIN KINASE 2B, CHLOROPLASTIC	coord: 114..275
None	No IPR available	PANTHER	PTHR45621	OS01G0588500 PROTEIN-RELATED	coord: 14..113
None	No IPR available	PANTHER	PTHR45621	OS01G0588500 PROTEIN-RELATED	coord: 114..275
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 116..192 e-value: 1.4E-13 score: 50.9
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..255 score: 13.898683
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 67..100
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 45..257

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG20g01960	Cp4.1LG20g01960	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG20g01960.1:exon:001	Cp4.1LG20g01960.1:exon:001	exon
Cp4.1LG20g01960.1:exon:002	Cp4.1LG20g01960.1:exon:002	exon
Cp4.1LG20g01960.1:exon:003	Cp4.1LG20g01960.1:exon:003	exon
Cp4.1LG20g01960.1:exon:004	Cp4.1LG20g01960.1:exon:004	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG20g01960.1:cds:001	Cp4.1LG20g01960.1:cds:001	CDS
Cp4.1LG20g01960.1:cds:002	Cp4.1LG20g01960.1:cds:002	CDS
Cp4.1LG20g01960.1:cds:003	Cp4.1LG20g01960.1:cds:003	CDS
Cp4.1LG20g01960.1:cds:004	Cp4.1LG20g01960.1:cds:004	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG20g01960.1:three_prime_utr:001	Cp4.1LG20g01960.1:three_prime_utr:001	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG20g01960.1	Cp4.1LG20g01960.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity