Cp4.1LG20g01870.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g01870.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	RING-type domain-containing protein
Location	Cp4.1LG20: 1133120 .. 1133797 (+)
Sequence length	678
RNA-Seq Expression	Cp4.1LG20g01870.1
Synteny	Cp4.1LG20g01870.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCACCGTCGCCGCCGCCGCCGCCGTCTCCGGCCTCCACTGCGAGGTTTGGCAGCCCCAAGACTTATCTGAAACTCACTGTTCTTTTTCTTCTTCTTCTTCTGAATTCCCCGAGTTCTTCATCGATTTCTATGTCTCCTCTTATGCAACTCAAGGCGACCAAATCATCAGCACTCTTCGTTACAAGAATTTCAGGCAAAGATGTGACGTTTTAACTCAAGATTCCTTCTCTTGGTCCGCAATTTCCACTATGCTATCCGAAACCAATGTGCCTTACCATCTTCAACCCTTTTTCATTCAGCAGATTTCCATTAGCGCCCGCGGAATTGCAACCGAACCCACTAACGCTCTCTCCCGCACCATTCCCATGGTCGTCGAGCTGGTGCTGCCTGAGGAGGCCATGGAGGATTCGGGTTATGGGTCGGACACCATTATGGGAACTGGGTCGCGCCGGGCGAGTAGAGCTTCGATTCAGGAAATGGAGAGAGTTGAAATGAATGGGGTTTTGAGTGATTGTGTGATTTGTTTGGATGATATCGTTTCAGTTGGGTCTGAAATTGATGCACTTCGAATGCCTTGTTTACATATTTACCATCGAAATTGTATTCACAAATGGCTGCAACTCAGCAACCGCTGCCCCTTGTGTCGGTTTCAGATGCCATTGGAAGAGGAATGA

mRNA sequence

ATGTCCACCGTCGCCGCCGCCGCCGCCGTCTCCGGCCTCCACTGCGAGGTTTGGCAGCCCCAAGACTTATCTGAAACTCACTGTTCTTTTTCTTCTTCTTCTTCTGAATTCCCCGAGTTCTTCATCGATTTCTATGTCTCCTCTTATGCAACTCAAGGCGACCAAATCATCAGCACTCTTCGTTACAAGAATTTCAGGCAAAGATGTGACGTTTTAACTCAAGATTCCTTCTCTTGGTCCGCAATTTCCACTATGCTATCCGAAACCAATGTGCCTTACCATCTTCAACCCTTTTTCATTCAGCAGATTTCCATTAGCGCCCGCGGAATTGCAACCGAACCCACTAACGCTCTCTCCCGCACCATTCCCATGGTCGTCGAGCTGGTGCTGCCTGAGGAGGCCATGGAGGATTCGGGTTATGGGTCGGACACCATTATGGGAACTGGGTCGCGCCGGGCGAGTAGAGCTTCGATTCAGGAAATGGAGAGAGTTGAAATGAATGGGGTTTTGAGTGATTGTGTGATTTGTTTGGATGATATCGTTTCAGTTGGGTCTGAAATTGATGCACTTCGAATGCCTTGTTTACATATTTACCATCGAAATTGTATTCACAAATGGCTGCAACTCAGCAACCGCTGCCCCTTGTGTCGGTTTCAGATGCCATTGGAAGAGGAATGA

Coding sequence (CDS)

ATGTCCACCGTCGCCGCCGCCGCCGCCGTCTCCGGCCTCCACTGCGAGGTTTGGCAGCCCCAAGACTTATCTGAAACTCACTGTTCTTTTTCTTCTTCTTCTTCTGAATTCCCCGAGTTCTTCATCGATTTCTATGTCTCCTCTTATGCAACTCAAGGCGACCAAATCATCAGCACTCTTCGTTACAAGAATTTCAGGCAAAGATGTGACGTTTTAACTCAAGATTCCTTCTCTTGGTCCGCAATTTCCACTATGCTATCCGAAACCAATGTGCCTTACCATCTTCAACCCTTTTTCATTCAGCAGATTTCCATTAGCGCCCGCGGAATTGCAACCGAACCCACTAACGCTCTCTCCCGCACCATTCCCATGGTCGTCGAGCTGGTGCTGCCTGAGGAGGCCATGGAGGATTCGGGTTATGGGTCGGACACCATTATGGGAACTGGGTCGCGCCGGGCGAGTAGAGCTTCGATTCAGGAAATGGAGAGAGTTGAAATGAATGGGGTTTTGAGTGATTGTGTGATTTGTTTGGATGATATCGTTTCAGTTGGGTCTGAAATTGATGCACTTCGAATGCCTTGTTTACATATTTACCATCGAAATTGTATTCACAAATGGCTGCAACTCAGCAACCGCTGCCCCTTGTGTCGGTTTCAGATGCCATTGGAAGAGGAATGA

Protein sequence

MSTVAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEFPEFFIDFYVSSYATQGDQIISTLRYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE

Homology

[Close]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.8e-11
Identity = 33/75 (44.00%), Postives = 45/75 (60.00%), Query Frame = 0


Query: 149 GSRRASRASIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIHKWLQ 208
           G   AS+ASI  M  VE++G   +CVICL++     SE     MPC H +H  CI KWL 
Sbjct: 89  GRPPASKASIDAMPIVEIDGCEGECVICLEE---WKSEETVKEMPCKHRFHGGCIEKWLG 148

Query: 209 LSNRCPLCRFQMPLE 224
               CP+CR++MP++
Sbjct: 149 FHGSCPVCRYEMPVD 160

[Close]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 32/76 (42.11%), Postives = 52/76 (68.42%), Query Frame = 0


Query: 149 GSRRASRASIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIHKWLQ 208
           G+  A + +++ +  V++  V+S C +CLDD+  VGS+  A +MPC H +H +CI  WL+
Sbjct: 176 GTPPAKKEAVEALPTVKIEEVVS-CSVCLDDL-EVGSQ--AKQMPCEHKFHSSCILPWLE 235

Query: 209 LSNRCPLCRFQMPLEE 225
           L + CP+CRF++P EE
Sbjct: 236 LHSSCPVCRFELPSEE 247

[Close]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.3e-10
Identity = 29/81 (35.80%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 149 GSRRASRASIQEMERV-----EMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 208
           G+  AS+++I+ +  V      +N   + C +C+DD    G+E  A +MPC H+YH++C+
Sbjct: 195 GTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDF-EEGTE--AKQMPCKHLYHKDCL 254

Query: 209 HKWLQLSNRCPLCRFQMPLEE 225
             WL+L N CP+CR ++P ++
Sbjct: 255 LPWLELHNSCPVCRHELPTDD 272

[Close]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.9e-10
Identity = 29/80 (36.25%), Postives = 51/80 (63.75%), Query Frame = 0


Query: 148 TGSRRASRASIQEMERVEMN----GVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 207
           +G+  AS+++I+ + RVE++    G  ++C +C +      +E +A  MPC H++H +CI
Sbjct: 170 SGNPPASKSAIESLPRVEISDCHIGSEANCAVCTE---IFETETEAREMPCKHLFHDDCI 229

Query: 208 HKWLQLSNRCPLCRFQMPLE 224
             WL + N CP+CRF++P E
Sbjct: 230 VPWLSIRNSCPVCRFELPSE 246

[Close]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.6e-09
Identity = 27/81 (33.33%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 149 GSRRASRASIQEMERVE-----MNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 208
           G+  AS+++I  +  V+     +   ++ C +C+D+    GS  D  +MPC H++H++C+
Sbjct: 187 GTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFED-GS--DVKQMPCKHVFHQDCL 246

Query: 209 HKWLQLSNRCPLCRFQMPLEE 225
             WL+L N CP+CRF++P ++
Sbjct: 247 LPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of Cp4.1LG20g01870.1 vs. NCBI nr
Match: XP_023519803.1 (RING-H2 finger protein ATL77-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453 bits (1166), Expect = 7.99e-161
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MSTVAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEFPEFFIDFYVSSYATQGDQIISTL 60
           MSTVAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEFPEFFIDFYVSSYATQGDQIISTL
Sbjct: 1   MSTVAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEFPEFFIDFYVSSYATQGDQIISTL 60

Query: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120
           RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR
Sbjct: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120

Query: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI 180
           TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI
Sbjct: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI 180

Query: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225
           VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE
Sbjct: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Cp4.1LG20g01870.1 vs. NCBI nr
Match: KAG6584266.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 447 bits (1150), Expect = 2.20e-158
Identity = 222/225 (98.67%), Postives = 222/225 (98.67%), Query Frame = 0


Query: 1   MSTVAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEFPEFFIDFYVSSYATQGDQIISTL 60
           MST AAAAAVSGLHCEVWQPQD SETHCSFSSSSSE PEFFIDFYVSSYATQGDQIISTL
Sbjct: 1   MSTAAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60

Query: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120
           RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR
Sbjct: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120

Query: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI 180
           TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI
Sbjct: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI 180

Query: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225
           VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE
Sbjct: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Cp4.1LG20g01870.1 vs. NCBI nr
Match: KAG7019862.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 447 bits (1150), Expect = 2.20e-158
Identity = 222/225 (98.67%), Postives = 222/225 (98.67%), Query Frame = 0


Query: 1   MSTVAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEFPEFFIDFYVSSYATQGDQIISTL 60
           MSTVAAAAAVSGLHCEVWQPQD SETHCSFSSSSSE PEFFIDFYVSSYATQGDQIISTL
Sbjct: 1   MSTVAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60

Query: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120
           RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR
Sbjct: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120

Query: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI 180
           TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASR SIQEMERVEMNGVLSDCVICLDDI
Sbjct: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDI 180

Query: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225
           VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE
Sbjct: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Cp4.1LG20g01870.1 vs. NCBI nr
Match: XP_023001523.1 (uncharacterized protein LOC111495638 [Cucurbita maxima])

HSP 1 Score: 443 bits (1140), Expect = 7.36e-157
Identity = 220/225 (97.78%), Postives = 220/225 (97.78%), Query Frame = 0


Query: 1   MSTVAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEFPEFFIDFYVSSYATQGDQIISTL 60
           MST AAAAAVSGLHCEVWQPQD SETHCSFSSSSSE PEFFIDFYVSSYATQGDQIISTL
Sbjct: 1   MSTAAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60

Query: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120
           RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR
Sbjct: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120

Query: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI 180
           TIPMVVELVLPEEAMEDS YGSDTIMGTGSRRASRASIQEMERVEMNG LSDCVICLDDI
Sbjct: 121 TIPMVVELVLPEEAMEDSSYGSDTIMGTGSRRASRASIQEMERVEMNGALSDCVICLDDI 180

Query: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225
           VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE
Sbjct: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Cp4.1LG20g01870.1 vs. NCBI nr
Match: XP_008437461.1 (PREDICTED: uncharacterized protein LOC103482872 [Cucumis melo] >TYJ99097.1 E3 ubiquitin-protein ligase [Cucumis melo var. makuwa])

HSP 1 Score: 382 bits (980), Expect = 1.68e-132
Identity = 188/224 (83.93%), Postives = 206/224 (91.96%), Query Frame = 0


Query: 4   VAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEF--PEFFIDFYVSSYATQGDQIISTLR 63
           ++AAAA+SGLHCEVWQPQD SE  CS SSSSS+F  PEFFIDFYVSSYAT GD++ISTLR
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCS-SSSSSQFQPPEFFIDFYVSSYATHGDEVISTLR 60

Query: 64  YKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRT 123
           YKNFRQRCDVLTQDSFSWSAIS+MLSETNVPYHLQPFFI QISI ARGIATEP NALSRT
Sbjct: 61  YKNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRT 120

Query: 124 IPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDIV 183
           IPMVVEL+LPE+AME SGYGS+  MG GS RASRASIQEMER+E++GVLSDCVICLD+I 
Sbjct: 121 IPMVVELMLPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEIDGVLSDCVICLDEIG 180

Query: 184 SVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225
           S+G EIDA+RMPCLH+YH NCIHKWL+LSNRCPLCRFQMPLEEE
Sbjct: 181 SIGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy TrEMBL
Match: A0A6J1KQS3 (uncharacterized protein LOC111495638 OS=Cucurbita maxima OX=3661 GN=LOC111495638 PE=4 SV=1)

HSP 1 Score: 443 bits (1140), Expect = 3.57e-157
Identity = 220/225 (97.78%), Postives = 220/225 (97.78%), Query Frame = 0


Query: 1   MSTVAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEFPEFFIDFYVSSYATQGDQIISTL 60
           MST AAAAAVSGLHCEVWQPQD SETHCSFSSSSSE PEFFIDFYVSSYATQGDQIISTL
Sbjct: 1   MSTAAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60

Query: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120
           RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR
Sbjct: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120

Query: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI 180
           TIPMVVELVLPEEAMEDS YGSDTIMGTGSRRASRASIQEMERVEMNG LSDCVICLDDI
Sbjct: 121 TIPMVVELVLPEEAMEDSSYGSDTIMGTGSRRASRASIQEMERVEMNGALSDCVICLDDI 180

Query: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225
           VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE
Sbjct: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy TrEMBL
Match: A0A5D3BJ05 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003020 PE=4 SV=1)

HSP 1 Score: 382 bits (980), Expect = 8.12e-133
Identity = 188/224 (83.93%), Postives = 206/224 (91.96%), Query Frame = 0


Query: 4   VAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEF--PEFFIDFYVSSYATQGDQIISTLR 63
           ++AAAA+SGLHCEVWQPQD SE  CS SSSSS+F  PEFFIDFYVSSYAT GD++ISTLR
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCS-SSSSSQFQPPEFFIDFYVSSYATHGDEVISTLR 60

Query: 64  YKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRT 123
           YKNFRQRCDVLTQDSFSWSAIS+MLSETNVPYHLQPFFI QISI ARGIATEP NALSRT
Sbjct: 61  YKNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRT 120

Query: 124 IPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDIV 183
           IPMVVEL+LPE+AME SGYGS+  MG GS RASRASIQEMER+E++GVLSDCVICLD+I 
Sbjct: 121 IPMVVELMLPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEIDGVLSDCVICLDEIG 180

Query: 184 SVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225
           S+G EIDA+RMPCLH+YH NCIHKWL+LSNRCPLCRFQMPLEEE
Sbjct: 181 SIGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy TrEMBL
Match: A0A1S3AU71 (uncharacterized protein LOC103482872 OS=Cucumis melo OX=3656 GN=LOC103482872 PE=4 SV=1)

HSP 1 Score: 382 bits (980), Expect = 8.12e-133
Identity = 188/224 (83.93%), Postives = 206/224 (91.96%), Query Frame = 0


Query: 4   VAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEF--PEFFIDFYVSSYATQGDQIISTLR 63
           ++AAAA+SGLHCEVWQPQD SE  CS SSSSS+F  PEFFIDFYVSSYAT GD++ISTLR
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCS-SSSSSQFQPPEFFIDFYVSSYATHGDEVISTLR 60

Query: 64  YKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRT 123
           YKNFRQRCDVLTQDSFSWSAIS+MLSETNVPYHLQPFFI QISI ARGIATEP NALSRT
Sbjct: 61  YKNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRT 120

Query: 124 IPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDIV 183
           IPMVVEL+LPE+AME SGYGS+  MG GS RASRASIQEMER+E++GVLSDCVICLD+I 
Sbjct: 121 IPMVVELMLPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEIDGVLSDCVICLDEIG 180

Query: 184 SVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225
           S+G EIDA+RMPCLH+YH NCIHKWL+LSNRCPLCRFQMPLEEE
Sbjct: 181 SIGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy TrEMBL
Match: A0A0A0LR28 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045460 PE=4 SV=1)

HSP 1 Score: 380 bits (975), Expect = 4.69e-132
Identity = 183/219 (83.56%), Postives = 200/219 (91.32%), Query Frame = 0


Query: 9   AVSGLHCEVWQPQDLSETHCSFSSSSSEF--PEFFIDFYVSSYATQGDQIISTLRYKNFR 68
           A+SGLHCEVWQPQD SE  CS SSSSS+F  PEFFIDFYVSSYAT GD++ISTLRYKNFR
Sbjct: 5   AISGLHCEVWQPQDFSEPSCSSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRYKNFR 64

Query: 69  QRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVV 128
           QRCDVLTQDSFSWSAIS+MLSETNVPYHLQPFFI QIS  ARGIATEP NALSRTIPMVV
Sbjct: 65  QRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISTRARGIATEPINALSRTIPMVV 124

Query: 129 ELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDIVSVGSE 188
           EL+LPE+AMEDSGYGS+  MG GS RASRASIQEMER+E++GVLSDCVICLD+I S+G E
Sbjct: 125 ELILPEDAMEDSGYGSEPHMGIGSGRASRASIQEMERIEIDGVLSDCVICLDEIGSIGCE 184

Query: 189 IDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225
           ID ++MPCLH+YH NCIHKWL+LSNRCPLCRFQMPLEEE
Sbjct: 185 IDVVQMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy TrEMBL
Match: A0A6J1ECV4 (E3 ubiquitin-protein ligase SIRP1-like OS=Cucurbita moschata OX=3662 GN=LOC111431384 PE=4 SV=1)

HSP 1 Score: 287 bits (735), Expect = 9.74e-97
Identity = 140/141 (99.29%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 85  MLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVELVLPEEAMEDSGYGSDT 144
           MLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVELVLPEE MEDSGYGSDT
Sbjct: 1   MLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVELVLPEEVMEDSGYGSDT 60

Query: 145 IMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIH 204
           IMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIH
Sbjct: 61  IMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIH 120

Query: 205 KWLQLSNRCPLCRFQMPLEEE 225
           KWLQLSNRCPLCRFQMPLEEE
Sbjct: 121 KWLQLSNRCPLCRFQMPLEEE 141

[Close]

BLAST of Cp4.1LG20g01870.1 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 3.4e-12
Identity = 33/75 (44.00%), Postives = 45/75 (60.00%), Query Frame = 0


Query: 149 GSRRASRASIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIHKWLQ 208
           G   AS+ASI  M  VE++G   +CVICL++     SE     MPC H +H  CI KWL 
Sbjct: 89  GRPPASKASIDAMPIVEIDGCEGECVICLEE---WKSEETVKEMPCKHRFHGGCIEKWLG 148

Query: 209 LSNRCPLCRFQMPLE 224
               CP+CR++MP++
Sbjct: 149 FHGSCPVCRYEMPVD 160

[Close]

BLAST of Cp4.1LG20g01870.1 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 65.9 bits (159), Expect = 4.9e-11
Identity = 29/80 (36.25%), Postives = 51/80 (63.75%), Query Frame = 0


Query: 148 TGSRRASRASIQEMERVEMN----GVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 207
           +G+  AS+++I+ + RVE++    G  ++C +C +      +E +A  MPC H++H +CI
Sbjct: 170 SGNPPASKSAIESLPRVEISDCHIGSEANCAVCTE---IFETETEAREMPCKHLFHDDCI 229

Query: 208 HKWLQLSNRCPLCRFQMPLE 224
             WL + N CP+CRF++P E
Sbjct: 230 VPWLSIRNSCPVCRFELPSE 246

[Close]

BLAST of Cp4.1LG20g01870.1 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.9e-10
Identity = 29/79 (36.71%), Postives = 46/79 (58.23%), Query Frame = 0


Query: 149 GSRRASRASIQEMERVE-----MNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 208
           G   AS  +I  +  V+     +   +S C +C+++ +  G   DA  +PC HIYH++CI
Sbjct: 195 GPPPASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGG---DATELPCKHIYHKDCI 254

Query: 209 HKWLQLSNRCPLCRFQMPL 223
             WL+L+N CP+CR  +PL
Sbjct: 255 VPWLRLNNSCPICRRDLPL 270

[Close]

BLAST of Cp4.1LG20g01870.1 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.9e-10
Identity = 27/81 (33.33%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 149 GSRRASRASIQEMERVE-----MNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 208
           G+  AS+++I  +  V+     +   ++ C +C+D+    GS  D  +MPC H++H++C+
Sbjct: 187 GTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFED-GS--DVKQMPCKHVFHQDCL 246

Query: 209 HKWLQLSNRCPLCRFQMPLEE 225
             WL+L N CP+CRF++P ++
Sbjct: 247 LPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of Cp4.1LG20g01870.1 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-10
Identity = 33/91 (36.26%), Postives = 55/91 (60.44%), Query Frame = 0


Query: 135 MEDSGYGSDTIMGTGSRRASRASIQEMERVEMNG----VLSDCVICLDDIVSVGSEIDAL 194
           +E SG G   I  +G+  AS+++I+ + RVE++       ++C +C  ++   G  I+  
Sbjct: 176 IEASGNG---IGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVC-TEVFEAG--IEGR 235

Query: 195 RMPCLHIYHRNCIHKWLQLSNRCPLCRFQMP 222
            MPC HI+H +CI  WL + N CP+CRF++P
Sbjct: 236 EMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g01870.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	4.8e-11	44.00	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q6AVN2	1.8e-10	42.11	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
P0CH30	5.3e-10	35.80	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q940T5	6.9e-10	36.25	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q8LPN7	2.6e-09	33.33	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]

BLAST of Cp4.1LG20g01870.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023519803.1	7.99e-161	100.00	RING-H2 finger protein ATL77-like [Cucurbita pepo subsp. pepo]	[more]
KAG6584266.1	2.20e-158	98.67	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7019862.1	2.20e-158	98.67	E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023001523.1	7.36e-157	97.78	uncharacterized protein LOC111495638 [Cucurbita maxima]	[more]
XP_008437461.1	1.68e-132	83.93	PREDICTED: uncharacterized protein LOC103482872 [Cucumis melo] >TYJ99097.1 E3 ub...	[more]

BLAST of Cp4.1LG20g01870.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KQS3	3.57e-157	97.78	uncharacterized protein LOC111495638 OS=Cucurbita maxima OX=3661 GN=LOC111495638...	[more]
A0A5D3BJ05	8.12e-133	83.93	E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3AU71	8.12e-133	83.93	uncharacterized protein LOC103482872 OS=Cucumis melo OX=3656 GN=LOC103482872 PE=...	[more]
A0A0A0LR28	4.69e-132	83.56	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045460 P...	[more]
A0A6J1ECV4	9.74e-97	99.29	E3 ubiquitin-protein ligase SIRP1-like OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of Cp4.1LG20g01870.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26800.1	3.4e-12	44.00	RING/U-box superfamily protein	[more]
AT5G59550.1	4.9e-11	36.25	zinc finger (C3HC4-type RING finger) family protein	[more]
AT1G60360.1	1.9e-10	36.71	RING/U-box superfamily protein	[more]
AT3G19950.1	1.9e-10	33.33	RING/U-box superfamily protein	[more]
AT3G46620.1	3.2e-10	36.26	zinc finger (C3HC4-type RING finger) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 173..216 e-value: 2.2E-7 score: 40.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 172..217 e-value: 7.4E-12 score: 45.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 173..217 score: 12.415513
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 172..217 e-value: 0.003 score: 11.7
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 142..222 e-value: 3.6E-17 score: 63.8
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 38..221
None	No IPR available	PANTHER	PTHR15710:SF138	FINGER PROTEIN, PUTATIVE-RELATED	coord: 38..221
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 172..221

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG20g01870	Cp4.1LG20g01870	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG20g01870.1:exon:001	Cp4.1LG20g01870.1:exon:001	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG20g01870.1:cds:001	Cp4.1LG20g01870.1:cds:001	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG20g01870.1	Cp4.1LG20g01870.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity