Cp4.1LG17g08370.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG17g08370.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	Cp4.1LG17: 5325500 .. 5326491 (-)
Sequence length	718
RNA-Seq Expression	Cp4.1LG17g08370.1
Synteny	Cp4.1LG17g08370.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGCATAAAAGGAAGTGGAAACCCATAAACTCTCTTCATCATTGCGTTCTTCAATTCACAGAAAATGGGGTTTCATTTTGGTTGCAAATGGCTTTTGTTAGCACTCCTGTTAGCCTCACCGGCGGTGAGCTTCATAGCAGGAGATGCAGAGAAAGACAGGGAGGAATGCACGCAGCAATTGGCTGGAATGGCGACGTGTCTAGCGTACGTGAGCGGTGACGCAAAGGCTCCAACGCCGGACTGTTGCAGTGGGCTGAAGGGCGTGCTTAAAAGCAACAAGAAATGCTTGTGCGTTATAGTGAAAGACCGAAATGACCCTGACTTGGGGCTTCAGATTAATGTCACCCTTGCCTTGGGCCTGCCTGATGTCTGCCACGCCCCTGCCAATGTCTCAAATTGTCCTGGTAACTACTCCGTGTTCTTCGCGTTCTTCGTGTTCTTCGTGTTCTTCGTGTTCTTTTTTTTAAATGGGGTTGTTGCAGCTCTATTAAACTTGCCACCCAATTCACCGGAGGCTCAAGTTTTCTATCAATTTGGTAAGGCTCAAAGCTCCTCTGCTGCTGCAGCTCCCATAAGTCCTTCTCCTGCCATTAACTCCACTGGTAATTTGATGATCAATTTTCTACAGTTTTTTTAGTTTGGAAAGCAAAATTAAGGTGTTGTTCTTTAAAATTGCAGGTGGAGGAGGCAGTAGAAATGGAACAGATAAGGGTGAAGCTTACACCATTGGAAACAAATGGCTGCCATTGAAGAGTTTGGTTGTTGCAGCTCTTTTGGTTTTGTTTTTGTTGGATTCTTAAACTGTTTTGAGTATATTATTCATGATTTTGTTGTGTTCTGTTCGAATATTAGTATTGTAATTCGTGTTTTTAAACGGTTCTGTTTTACACGTGGAGTTGGAGATGGAATTTATATTGTGAGTGTCAAATTTTGGACAAATAAGGTAACGTAGCTGACGTTTACGTGACAAACAACAATAAAATTTGTTTGGG

mRNA sequence

ACGCATAAAAGGAAGTGGAAACCCATAAACTCTCTTCATCATTGCGTTCTTCAATTCACAGAAAATGGGGTTTCATTTTGGTTGCAAATGGCTTTTGTTAGCACTCCTGTTAGCCTCACCGGCGGTGAGCTTCATAGCAGGAGATGCAGAGAAAGACAGGGAGGAATGCACGCAGCAATTGGCTGGAATGGCGACGTGTCTAGCGTACGTGAGCGGTGACGCAAAGGCTCCAACGCCGGACTGTTGCAGTGGGCTGAAGGGCGTGCTTAAAAGCAACAAGAAATGCTTGTGCGTTATAGTGAAAGACCGAAATGACCCTGACTTGGGGCTTCAGATTAATGTCACCCTTGCCTTGGGCCTGCCTGATGTCTGCCACGCCCCTGCCAATGTCTCAAATTGTCCTGGTGGAGGAGGCAGTAGAAATGGAACAGATAAGGGTGAAGCTTACACCATTGGAAACAAATGGCTGCCATTGAAGAGTTTGGTTGTTGCAGCTCTTTTGGTTTTGTTTTTGTTGGATTCTTAAACTGTTTTGAGTATATTATTCATGATTTTGTTGTGTTCTGTTCGAATATTAGTATTGTAATTCGTGTTTTTAAACGGTTCTGTTTTACACGTGGAGTTGGAGATGGAATTTATATTGTGAGTGTCAAATTTTGGACAAATAAGGTAACGTAGCTGACGTTTACGTGACAAACAACAATAAAATTTGTTTGGG

Coding sequence (CDS)

ATGGGGTTTCATTTTGGTTGCAAATGGCTTTTGTTAGCACTCCTGTTAGCCTCACCGGCGGTGAGCTTCATAGCAGGAGATGCAGAGAAAGACAGGGAGGAATGCACGCAGCAATTGGCTGGAATGGCGACGTGTCTAGCGTACGTGAGCGGTGACGCAAAGGCTCCAACGCCGGACTGTTGCAGTGGGCTGAAGGGCGTGCTTAAAAGCAACAAGAAATGCTTGTGCGTTATAGTGAAAGACCGAAATGACCCTGACTTGGGGCTTCAGATTAATGTCACCCTTGCCTTGGGCCTGCCTGATGTCTGCCACGCCCCTGCCAATGTCTCAAATTGTCCTGGTGGAGGAGGCAGTAGAAATGGAACAGATAAGGGTGAAGCTTACACCATTGGAAACAAATGGCTGCCATTGAAGAGTTTGGTTGTTGCAGCTCTTTTGGTTTTGTTTTTGTTGGATTCTTAA

Protein sequence

MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPGGGGSRNGTDKGEAYTIGNKWLPLKSLVVAALLVLFLLDS

Homology

[Close]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.2e-36
Identity = 71/102 (69.61%), Postives = 84/102 (82.35%), Query Frame = 0


Query: 12  LALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSN 71
           +AL++ +  V+  A D  KD+EECT+QL GMATCL YV G AK+PTPDCCSGLK VL SN
Sbjct: 13  IALIVVAMVVA-AADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSN 72

Query: 72  KKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP 114
           KKCLCVI++DRNDPDLGLQINV+LAL LP VCHA A+V+ CP
Sbjct: 73  KKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCP 113

[Close]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.6e-34
Identity = 63/102 (61.76%), Postives = 80/102 (78.43%), Query Frame = 0


Query: 12  LALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSN 71
           +AL++ +  V     D  KD+EECT QL GMATCL YV G AK+PTPDCCSGLK V+ S+
Sbjct: 13  IALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSD 72

Query: 72  KKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP 114
            KCLC+I+++RNDPDLGLQ+NV+LAL LP VCHA A+++ CP
Sbjct: 73  MKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCP 114

[Close]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy Swiss-Prot
Match: F4I082 (Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=3702 GN=LTPG6 PE=2 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 2.3e-25
Identity = 49/104 (47.12%), Postives = 74/104 (71.15%), Query Frame = 0


Query: 10  LLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLK 69
           L + +++ S  + F   D  +DREECT QL  ++TC+ YV GDAKAPT DCC+G   V++
Sbjct: 8   LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 67

Query: 70  SNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPA-NVSNC 113
            ++KC+C++V+D++DP LG++IN TLA  LP  CH  A N+++C
Sbjct: 68  KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDC 111

[Close]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy Swiss-Prot
Match: Q9M2G1 (Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3702 GN=LTPG22 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.9e-17
Identity = 43/107 (40.19%), Postives = 65/107 (60.75%), Query Frame = 0


Query: 11  LLALLLASPAVSF--IAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVL 70
           +LAL + + AV F  +  +  +D + C   ++ + +CL +V+  AKAP   CCS LK  +
Sbjct: 13  MLALCI-TVAVMFLGVRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKI 72

Query: 71  K--SNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP 114
                +KCLC +VKDR+DP LG +++   A+ LP  CH PAN+S CP
Sbjct: 73  DKGQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCP 118

[Close]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy Swiss-Prot
Match: Q9LJ86 (Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=3702 GN=LTPG5 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.2e-11
Identity = 35/97 (36.08%), Postives = 52/97 (53.61%), Query Frame = 0


Query: 32  REECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSNKKCLCVIVKDRNDPDLGLQI 91
           +  CT  L  M+ CL Y++G++ +P   CC+ L  V++S+  CLC ++ +     LG+ +
Sbjct: 25  QSSCTNALISMSPCLNYITGNSTSPNQQCCNQLSRVVQSSPDCLCQVL-NGGGSQLGINV 84

Query: 92  NVTLALGLPDVCHAPA-NVSNCPGGGGSRNGTDKGEA 128
           N T ALGLP  C+     VS C  GGG    T    A
Sbjct: 85  NQTQALGLPRACNVQTPPVSRCNTGGGGGGSTSDSPA 120

[Close]

BLAST of Cp4.1LG17g08370.1 vs. NCBI nr
Match: XP_023513729.1 (protein YLS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 294 bits (752), Expect = 1.77e-99
Identity = 153/193 (79.27%), Postives = 153/193 (79.27%), Query Frame = 0


Query: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP------- 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP       
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 ---------------------------------GGGGSRNGTDKGEAYTIGNKWLPLKSL 153
                                            GGGGSRNGTDKGEAYTIGNKWLPLKSL
Sbjct: 121 NSPEAQVFYQFGKAQSSSAAAAPISPSPAINSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180

[Close]

BLAST of Cp4.1LG17g08370.1 vs. NCBI nr
Match: XP_022959693.1 (protein YLS3-like [Cucurbita moschata])

HSP 1 Score: 291 bits (746), Expect = 1.45e-98
Identity = 152/193 (78.76%), Postives = 152/193 (78.76%), Query Frame = 0


Query: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHF CKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP------- 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP       
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 ---------------------------------GGGGSRNGTDKGEAYTIGNKWLPLKSL 153
                                            GGGGSRNGTDKGEAYTIGNKWLPLKSL
Sbjct: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180

[Close]

BLAST of Cp4.1LG17g08370.1 vs. NCBI nr
Match: KAG6593339.1 (Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025687.1 Protein YLS3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 286 bits (732), Expect = 2.12e-96
Identity = 149/192 (77.60%), Postives = 150/192 (78.12%), Query Frame = 0


Query: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPG------ 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP       
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 ---------------------------------GGGSRNGTDKGEAYTIGNKWLPLKSLV 153
                                            GGGSRNGTDKGEAYTIGNKWLPLK+LV
Sbjct: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAINSTGGGSRNGTDKGEAYTIGNKWLPLKTLV 180

[Close]

BLAST of Cp4.1LG17g08370.1 vs. NCBI nr
Match: XP_023004343.1 (protein YLS3-like [Cucurbita maxima])

HSP 1 Score: 285 bits (729), Expect = 6.28e-96
Identity = 147/193 (76.17%), Postives = 149/193 (77.20%), Query Frame = 0


Query: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHFGCKWLLL LLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFGCKWLLLTLLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP------- 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVS CP       
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSTCPALLNLPP 120

Query: 121 ---------------------------------GGGGSRNGTDKGEAYTIGNKWLPLKSL 153
                                            GGGGSRNGTDKGEAYT+GNKWLPLK+L
Sbjct: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAINSTGGGGSRNGTDKGEAYTVGNKWLPLKTL 180

[Close]

BLAST of Cp4.1LG17g08370.1 vs. NCBI nr
Match: XP_038899115.1 (non-specific lipid transfer protein GPI-anchored 13 [Benincasa hispida])

HSP 1 Score: 237 bits (604), Expect = 7.54e-77
Identity = 124/194 (63.92%), Postives = 132/194 (68.04%), Query Frame = 0


Query: 2   GFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCC 61
           GFHF  KWLLL LLL SP VSF+ GD EKD+EECTQQLAGMATCL YVSG AKAPTPDCC
Sbjct: 3   GFHFTWKWLLLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGGAKAPTPDCC 62

Query: 62  SGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPG------- 121
           SGLK VLK+NKKCLCVI+KDRNDPDLGLQINVTLALGLPD+CHA ANVSNCP        
Sbjct: 63  SGLKEVLKNNKKCLCVIIKDRNDPDLGLQINVTLALGLPDICHATANVSNCPALLNLPPN 122

Query: 122 -----------------------------------GGGSRNGTDKGEAYTIGNKWLPLKS 153
                                              GGGSR G DKG AY IG KWLPL++
Sbjct: 123 SAEAQVFYQLGKGNSSSSAVAPAPISPSPTINSTVGGGSRIGPDKGGAYNIGKKWLPLET 182

[Close]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy TrEMBL
Match: A0A6J1H6P3 (protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111460690 PE=3 SV=1)

HSP 1 Score: 291 bits (746), Expect = 7.04e-99
Identity = 152/193 (78.76%), Postives = 152/193 (78.76%), Query Frame = 0


Query: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHF CKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP------- 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP       
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 ---------------------------------GGGGSRNGTDKGEAYTIGNKWLPLKSL 153
                                            GGGGSRNGTDKGEAYTIGNKWLPLKSL
Sbjct: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180

[Close]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy TrEMBL
Match: A0A6J1KZ76 (protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111497688 PE=3 SV=1)

HSP 1 Score: 285 bits (729), Expect = 3.04e-96
Identity = 147/193 (76.17%), Postives = 149/193 (77.20%), Query Frame = 0


Query: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHFGCKWLLL LLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFGCKWLLLTLLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP------- 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVS CP       
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSTCPALLNLPP 120

Query: 121 ---------------------------------GGGGSRNGTDKGEAYTIGNKWLPLKSL 153
                                            GGGGSRNGTDKGEAYT+GNKWLPLK+L
Sbjct: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAINSTGGGGSRNGTDKGEAYTVGNKWLPLKTL 180

[Close]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy TrEMBL
Match: A0A6J1D8S3 (protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111018659 PE=3 SV=1)

HSP 1 Score: 225 bits (574), Expect = 1.34e-72
Identity = 122/196 (62.24%), Postives = 129/196 (65.82%), Query Frame = 0


Query: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           M FHF  K LLL LLL SPAV  +  DAEKD+EECTQQLAGMATCL YVSGDAK+PTPDC
Sbjct: 1   MDFHFAFKLLLLTLLLTSPAVRLVTADAEKDKEECTQQLAGMATCLPYVSGDAKSPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPG------ 120
           C+GLK VLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPD CHA ANVSNCP       
Sbjct: 61  CNGLKEVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDSCHASANVSNCPALLNLPP 120

Query: 121 -------------------------------------GGGSRNGTDKGEAYTIGNKWLPL 153
                                                GGGSR G DKGEA   GNKWLPL
Sbjct: 121 NSPEAQVFYQLGKSNSSSAAASAAAPISPSSTTNSTVGGGSRIGPDKGEACYRGNKWLPL 180

[Close]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy TrEMBL
Match: A0A1S3CDS0 (protein YLS3-like OS=Cucumis melo OX=3656 GN=LOC103499877 PE=4 SV=1)

HSP 1 Score: 194 bits (492), Expect = 3.13e-60
Identity = 108/190 (56.84%), Postives = 119/190 (62.63%), Query Frame = 0


Query: 2   GFHFGCKWLLLALLLASPA-VSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 61
           GF    KW LL LLL SP+ V F+ GD +KD+EECT QLAGMATCL YVSGDAK PTPDC
Sbjct: 3   GFRLCFKWFLLMLLLRSPSSVRFVGGDDKKDKEECTPQLAGMATCLPYVSGDAKTPTPDC 62

Query: 62  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP------- 121
           CSGLK VLK+NKKCLCVIVKDRNDPDLGLQINVTLALGLPD+CHA ANVSNCP       
Sbjct: 63  CSGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDICHATANVSNCPALLNLPS 122

Query: 122 -----------GGGGSRN----------------------GTDKGEAYTIGNKWLPLKSL 150
                      G G S +                      G+ K   Y IG KW+P + L
Sbjct: 123 NSSEAQVFYQLGKGKSSSALAPAPIISPSSPATISSTVGGGSTKSGGYKIGKKWVPSEIL 182

[Close]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy TrEMBL
Match: A0A0A0K6P6 (Non-specific lipid-transfer protein OS=Cucumis sativus OX=3659 GN=Csa_7G430130 PE=4 SV=1)

HSP 1 Score: 184 bits (466), Expect = 3.25e-56
Identity = 106/193 (54.92%), Postives = 121/193 (62.69%), Query Frame = 0


Query: 4   HFGCKWL-LLALLLASPA-VSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCC 63
            F  KWL L+ LLL SP+ V F+ GD +KDREECT QLAGMATCL YVSGDAKAPTPDCC
Sbjct: 6   RFSFKWLWLIMLLLRSPSWVRFVGGDDKKDREECTPQLAGMATCLPYVSGDAKAPTPDCC 65

Query: 64  SGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPG------- 123
           SGLK VL+++KKCLCVIV+DRNDPDLGLQINVTLAL LPD+CHA ANVSNCP        
Sbjct: 66  SGLKEVLQNDKKCLCVIVRDRNDPDLGLQINVTLALSLPDICHATANVSNCPALLNMPSN 125

Query: 124 -------------------------------------GGGSRNGTDKGEAYTIGNKWLPL 150
                                                GGGS++G + GE Y  G K + L
Sbjct: 126 SSDAQVFYQLGKGKSSSALAPAPMLSPSSSPTINSTVGGGSKSGAE-GEGYKRGKKRVAL 185

[Close]

BLAST of Cp4.1LG17g08370.1 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 1.6e-37
Identity = 71/102 (69.61%), Postives = 84/102 (82.35%), Query Frame = 0


Query: 12  LALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSN 71
           +AL++ +  V+  A D  KD+EECT+QL GMATCL YV G AK+PTPDCCSGLK VL SN
Sbjct: 13  IALIVVAMVVA-AADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSN 72

Query: 72  KKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP 114
           KKCLCVI++DRNDPDLGLQINV+LAL LP VCHA A+V+ CP
Sbjct: 73  KKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCP 113

[Close]

BLAST of Cp4.1LG17g08370.1 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 3.3e-35
Identity = 63/102 (61.76%), Postives = 80/102 (78.43%), Query Frame = 0


Query: 12  LALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSN 71
           +AL++ +  V     D  KD+EECT QL GMATCL YV G AK+PTPDCCSGLK V+ S+
Sbjct: 13  IALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSD 72

Query: 72  KKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP 114
            KCLC+I+++RNDPDLGLQ+NV+LAL LP VCHA A+++ CP
Sbjct: 73  MKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCP 114

[Close]

BLAST of Cp4.1LG17g08370.1 vs. TAIR 10
Match: AT1G55260.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 1.6e-26
Identity = 49/104 (47.12%), Postives = 74/104 (71.15%), Query Frame = 0


Query: 10  LLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLK 69
           L + +++ S  + F   D  +DREECT QL  ++TC+ YV GDAKAPT DCC+G   V++
Sbjct: 51  LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 110

Query: 70  SNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPA-NVSNC 113
            ++KC+C++V+D++DP LG++IN TLA  LP  CH  A N+++C
Sbjct: 111 KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDC 154

[Close]

BLAST of Cp4.1LG17g08370.1 vs. TAIR 10
Match: AT1G55260.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 1.6e-26
Identity = 49/104 (47.12%), Postives = 74/104 (71.15%), Query Frame = 0


Query: 10  LLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLK 69
           L + +++ S  + F   D  +DREECT QL  ++TC+ YV GDAKAPT DCC+G   V++
Sbjct: 51  LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 110

Query: 70  SNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPA-NVSNC 113
            ++KC+C++V+D++DP LG++IN TLA  LP  CH  A N+++C
Sbjct: 111 KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDC 154

[Close]

BLAST of Cp4.1LG17g08370.1 vs. TAIR 10
Match: AT3G58550.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 2.8e-18
Identity = 43/107 (40.19%), Postives = 65/107 (60.75%), Query Frame = 0


Query: 11  LLALLLASPAVSF--IAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVL 70
           +LAL + + AV F  +  +  +D + C   ++ + +CL +V+  AKAP   CCS LK  +
Sbjct: 13  MLALCI-TVAVMFLGVRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKI 72

Query: 71  K--SNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCP 114
                +KCLC +VKDR+DP LG +++   A+ LP  CH PAN+S CP
Sbjct: 73  DKGQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCP 118

The following BLAST results are available for this feature:

BLAST of Cp4.1LG17g08370.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64865	2.2e-36	69.61	Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3...	[more]
O64864	4.6e-34	61.76	Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3...	[more]
F4I082	2.3e-25	47.12	Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=37...	[more]
Q9M2G1	3.9e-17	40.19	Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3...	[more]
Q9LJ86	3.2e-11	36.08	Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of Cp4.1LG17g08370.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023513729.1	1.77e-99	79.27	protein YLS3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022959693.1	1.45e-98	78.76	protein YLS3-like [Cucurbita moschata]	[more]
KAG6593339.1	2.12e-96	77.60	Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025687.1 Prot...	[more]
XP_023004343.1	6.28e-96	76.17	protein YLS3-like [Cucurbita maxima]	[more]
XP_038899115.1	7.54e-77	63.92	non-specific lipid transfer protein GPI-anchored 13 [Benincasa hispida]	[more]

BLAST of Cp4.1LG17g08370.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6P3	7.04e-99	78.76	protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111460690 PE=3 SV=1	[more]
A0A6J1KZ76	3.04e-96	76.17	protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111497688 PE=3 SV=1	[more]
A0A6J1D8S3	1.34e-72	62.24	protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111018659 PE=3 SV=1	[more]
A0A1S3CDS0	3.13e-60	56.84	protein YLS3-like OS=Cucumis melo OX=3656 GN=LOC103499877 PE=4 SV=1	[more]
A0A0A0K6P6	3.25e-56	54.92	Non-specific lipid-transfer protein OS=Cucumis sativus OX=3659 GN=Csa_7G430130 P...	[more]

BLAST of Cp4.1LG17g08370.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44300.1	1.6e-37	69.61	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44290.1	3.3e-35	61.76	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.1	1.6e-26	47.12	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.2	1.6e-26	47.12	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G58550.1	2.8e-18	40.19	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000528	Plant non-specific lipid-transfer protein/Par allergen	PRINTS	PR00382	LIPIDTRNSFER	coord: 55..69 score: 33.23 coord: 90..107 score: 28.63 coord: 34..50 score: 28.33
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 35..112 e-value: 1.3E-5 score: 34.7
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 16..112 e-value: 1.9E-14 score: 53.6
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 32..111 e-value: 3.6E-15 score: 57.6
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 34..113
None	No IPR available	PANTHER	PTHR33044:SF94	PROTEIN YLS3	coord: 12..116
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 42..104 e-value: 5.24337E-16 score: 65.911
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 12..116

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG17g08370	Cp4.1LG17g08370	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG17g08370.1:three_prime_utr:001	Cp4.1LG17g08370.1:three_prime_utr:001	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG17g08370.1:exon:002	Cp4.1LG17g08370.1:exon:002	exon
Cp4.1LG17g08370.1:exon:001	Cp4.1LG17g08370.1:exon:001	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG17g08370.1:cds:002	Cp4.1LG17g08370.1:cds:002	CDS
Cp4.1LG17g08370.1:cds:001	Cp4.1LG17g08370.1:cds:001	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG17g08370.1:five_prime_utr:001	Cp4.1LG17g08370.1:five_prime_utr:001	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG17g08370.1	Cp4.1LG17g08370.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006869	lipid transport
biological_process	GO:0008643	carbohydrate transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0008289	lipid binding
molecular_function	GO:0015293	symporter activity