Cp4.1LG15g02010.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG15g02010.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	E3 ubiquitin-protein ligase RNF14
Location	Cp4.1LG15: 1630985 .. 1632072 (-)
Sequence length	621
RNA-Seq Expression	Cp4.1LG15g02010.1
Synteny	Cp4.1LG15g02010.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTCTCTGTCCGATGCCATCATGCTCTTGTTCTGTATCCTCCAATTGAGCATTTCCAGCTATCGTGCTCGCAATTGTTCGAACATCTTACAAATGGTACTCTTCAACCTAGTTTTCCTTTTCCTTTCCCCATAGCGATTGATTCAACTCTATAAATAGCTTTGGCGAACTTCATCAAGTTTTGAATCAAGCTATTGGTCATTTCCTCTAGCATTACCGAAACCCGGGTCTCGATCTCTTTACTACATTCCTCTGTTTCTTATTGCTAATAATTTGCTTGATCTAGATCTCAACACCATCATGCTTTCATTCTTTATCCTCCAATTGAGCGTTTCCAGCTATCGTGCTTGCAATTGTTCGAACATCTTACAAATGGTACTCTTCAACCTAGTTTTCCTTTTCCTTTCCCCATAGCGATTGATTCAACTCTATAAATAGCTTTGGCGTGCTCCACATCAAGTTTTGAATCAAGCTATTGGTCTTTTCTTCTAGCATTACCGAAACCCGGGTCTCGATCTCTCTACTACATTCCTCCATTTCTTATCGCATATAATTTGCTTGATCTAGATCTTAACATGCTGATGAGTGAAAAAAAAATACTGTGTTTGTGATTCGACTTTATTTTGTTTTCATTCTTTTAATGAATGAACAGGTTGGCAATAACAATCACATACGTTGCTGGTCGTGTCAAAACCACTACTGCTATCTATGCAAAAAAGTTGTGAAGCGCAGCTCCCAGCATTATGGACCCAAGGGCTGCAAACAACACACAGCTGGTTAGTTTTACTGCTACACTTCCAAAAGCTAAGGCTTATTACTCATCAAAATTAAAGGGAAGAGCCATTTACAATCTCGTAAAGCTTTTATCTTCTTCTCCCACTTTTTCTTACCCCTTTTTCTTTCCCTCTTTCACTTCATTTTTCTGACTGATTTAGTTTAGTTGATCATAGGATAATGTTTTATTCAGCACTTCTTTTTTTTGTTGAAACAGATAGCAGAAATTTTTTAAGGTTTACCCATGTAAAGGTATTAGAATTACAGGCCTGTTCTAATAATACCCATGTTGTGGTTCACGTGTTTAGTT

mRNA sequence

ATGGATTTCTCTGTCCGATGCCATCATGCTCTTGTTCTGTATCCTCCAATTGAGCATTTCCAGCTATCGTGCTCGCAATTGTTCGAACATCTTACAAATGATCTCAACACCATCATGCTTTCATTCTTTATCCTCCAATTGAGCGTTTCCAGCTATCGTGCTTGCAATTGTTCGAACATCTTACAAATGGTTGGCAATAACAATCACATACGTTGCTGGTCGTGTCAAAACCACTACTGCTATCTATGCAAAAAAGTTGTGAAGCGCAGCTCCCAGCATTATGGACCCAAGGGCTGCAAACAACACACAGCTGGTTAGTTTTACTGCTACACTTCCAAAAGCTAAGGCTTATTACTCATCAAAATTAAAGGGAAGAGCCATTTACAATCTCGTAAAGCTTTTATCTTCTTCTCCCACTTTTTCTTACCCCTTTTTCTTTCCCTCTTTCACTTCATTTTTCTGACTGATTTAGTTTAGTTGATCATAGGATAATGTTTTATTCAGCACTTCTTTTTTTTGTTGAAACAGATAGCAGAAATTTTTTAAGGTTTACCCATGTAAAGGTATTAGAATTACAGGCCTGTTCTAATAATACCCATGTTGTGGTTCACGTGTTTAGTT

Coding sequence (CDS)

ATGGATTTCTCTGTCCGATGCCATCATGCTCTTGTTCTGTATCCTCCAATTGAGCATTTCCAGCTATCGTGCTCGCAATTGTTCGAACATCTTACAAATGATCTCAACACCATCATGCTTTCATTCTTTATCCTCCAATTGAGCGTTTCCAGCTATCGTGCTTGCAATTGTTCGAACATCTTACAAATGGTTGGCAATAACAATCACATACGTTGCTGGTCGTGTCAAAACCACTACTGCTATCTATGCAAAAAAGTTGTGAAGCGCAGCTCCCAGCATTATGGACCCAAGGGCTGCAAACAACACACAGCTGGTTAG

Protein sequence

MDFSVRCHHALVLYPPIEHFQLSCSQLFEHLTNDLNTIMLSFFILQLSVSSYRACNCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG

Homology

[Close]

BLAST of Cp4.1LG15g02010.1 vs. NCBI nr
Match: XP_023554779.1 (E3 ubiquitin-protein ligase RNF14 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 107 bits (267), Expect = 1.86e-24
Identity = 60/110 (54.55%), Postives = 67/110 (60.91%), Query Frame = 0


Query: 3   FSVRCHHALVLYPPIEHFQLSCSQLFEHLT-----NDLNTIMLSFFI-LQLSVSSYRAC- 62
           F  RC  A+  Y   +HF+    +LF   T       +N   L   I  +L   +   C 
Sbjct: 540 FCYRCSKAIDGY---DHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCP 599

Query: 63  NCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 105
           NC  I   VGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG
Sbjct: 600 NCGQINAKVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 646

[Close]

BLAST of Cp4.1LG15g02010.1 vs. NCBI nr
Match: KAG6571648.1 (E3 ubiquitin-protein ligase RNF14, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 107 bits (266), Expect = 2.48e-24
Identity = 45/50 (90.00%), Postives = 45/50 (90.00%), Query Frame = 0


Query: 56  NCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 105
           NC  I   VGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG
Sbjct: 570 NCGQINAKVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 619

[Close]

BLAST of Cp4.1LG15g02010.1 vs. NCBI nr
Match: XP_022963554.1 (E3 ubiquitin-protein ligase RNF14 [Cucurbita moschata])

HSP 1 Score: 105 bits (263), Expect = 6.51e-24
Identity = 44/50 (88.00%), Postives = 45/50 (90.00%), Query Frame = 0


Query: 56  NCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 105
           NC  I   VGNNNHIRCW+CQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG
Sbjct: 597 NCGQINAKVGNNNHIRCWACQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 646

[Close]

BLAST of Cp4.1LG15g02010.1 vs. NCBI nr
Match: KAG7011377.1 (E3 ubiquitin-protein ligase RNF14 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 105 bits (263), Expect = 6.51e-24
Identity = 44/50 (88.00%), Postives = 45/50 (90.00%), Query Frame = 0


Query: 56  NCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 105
           NC  I   VGNNNHIRCW+CQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG
Sbjct: 597 NCGQINAKVGNNNHIRCWACQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 646

[Close]

BLAST of Cp4.1LG15g02010.1 vs. NCBI nr
Match: XP_022967205.1 (E3 ubiquitin-protein ligase RNF14 [Cucurbita maxima])

HSP 1 Score: 105 bits (262), Expect = 8.89e-24
Identity = 43/50 (86.00%), Postives = 45/50 (90.00%), Query Frame = 0


Query: 56  NCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 105
           NC  I   VGNNNHIRCW+CQNHYCYLCKK+VKRSSQHYGPKGCKQHTAG
Sbjct: 597 NCGQINAKVGNNNHIRCWACQNHYCYLCKKIVKRSSQHYGPKGCKQHTAG 646

[Close]

BLAST of Cp4.1LG15g02010.1 vs. ExPASy TrEMBL
Match: A0A6J1HKI4 (E3 ubiquitin-protein ligase RNF14 OS=Cucurbita moschata OX=3662 GN=LOC111463847 PE=3 SV=1)

HSP 1 Score: 105 bits (263), Expect = 3.15e-24
Identity = 44/50 (88.00%), Postives = 45/50 (90.00%), Query Frame = 0


Query: 56  NCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 105
           NC  I   VGNNNHIRCW+CQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG
Sbjct: 597 NCGQINAKVGNNNHIRCWACQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 646

[Close]

BLAST of Cp4.1LG15g02010.1 vs. ExPASy TrEMBL
Match: A0A6J1HTS2 (E3 ubiquitin-protein ligase RNF14 OS=Cucurbita maxima OX=3661 GN=LOC111466811 PE=3 SV=1)

HSP 1 Score: 105 bits (262), Expect = 4.30e-24
Identity = 43/50 (86.00%), Postives = 45/50 (90.00%), Query Frame = 0


Query: 56  NCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 105
           NC  I   VGNNNHIRCW+CQNHYCYLCKK+VKRSSQHYGPKGCKQHTAG
Sbjct: 597 NCGQINAKVGNNNHIRCWACQNHYCYLCKKIVKRSSQHYGPKGCKQHTAG 646

[Close]

BLAST of Cp4.1LG15g02010.1 vs. ExPASy TrEMBL
Match: A0A314YBI4 (E3 ubiquitin-protein ligase RNF14 OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_39274 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 2.50e-23
Identity = 53/110 (48.18%), Postives = 67/110 (60.91%), Query Frame = 0


Query: 3   FSVRCHHALVLYPPIEHFQLSCSQLF--EHLTN---DLNTI-MLSFFILQLSVSSYRAC- 62
           F  RC  A+  Y   +HF+    +LF  E + N    +N   +L     +L  +  + C 
Sbjct: 21  FCYRCDKAIEGY---DHFRDGACELFPQEAIQNWEAQMNPRQVLGQIQAELFAAHGQLCP 80

Query: 63  NCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 105
           NC      +GNNNH+ CW+CQNHYCYLCKK+VKRSSQHYG KGCKQHTAG
Sbjct: 81  NCGQFNAKIGNNNHMFCWACQNHYCYLCKKIVKRSSQHYGRKGCKQHTAG 127

[Close]

BLAST of Cp4.1LG15g02010.1 vs. ExPASy TrEMBL
Match: A0A6J1DZW6 (E3 ubiquitin-protein ligase RNF14 OS=Momordica charantia OX=3673 GN=LOC111025870 PE=3 SV=1)

HSP 1 Score: 100 bits (248), Expect = 3.37e-22
Identity = 40/48 (83.33%), Postives = 43/48 (89.58%), Query Frame = 0


Query: 56  NCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHT 103
           NC  +   VGNNNHIRCW+CQNHYCYLC+KVVKRSSQHYGPKGCKQHT
Sbjct: 587 NCGQLNAKVGNNNHIRCWACQNHYCYLCRKVVKRSSQHYGPKGCKQHT 634

[Close]

BLAST of Cp4.1LG15g02010.1 vs. ExPASy TrEMBL
Match: A0A1S3CQI9 (E3 ubiquitin-protein ligase RNF14 OS=Cucumis melo OX=3656 GN=LOC103503565 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 6.31e-22
Identity = 54/110 (49.09%), Postives = 65/110 (59.09%), Query Frame = 0


Query: 3   FSVRCHHALVLYPPIEHFQLSCSQLF-----EHLTNDLNTI-MLSFFILQLSVSSYRAC- 62
           F  RC  A+  Y   +HF+    +LF     +     +N   +L     +L   + R C 
Sbjct: 535 FCYRCSKAIDGY---DHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPENGRPCP 594

Query: 63  NCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 105
           NC  +   VGNNNHIRCWSCQ HYCYLC+K VKRSSQHYGPKGCKQHT G
Sbjct: 595 NCGQLNAKVGNNNHIRCWSCQIHYCYLCRKAVKRSSQHYGPKGCKQHTVG 641

[Close]

BLAST of Cp4.1LG15g02010.1 vs. TAIR 10
Match: AT1G32340.1 (NDR1/HIN1-like 8 )

HSP 1 Score: 88.2 bits (217), Expect = 4.3e-18
Identity = 48/116 (41.38%), Postives = 61/116 (52.59%), Query Frame = 0


Query: 3   FSVRCHHALVLYPPIEHFQLSCSQLF-EHLTNDLNTIMLSFFIL------------QLSV 62
           F  RC+ A+  Y   EHF     +LF +    + N +M    ++            Q   
Sbjct: 576 FCYRCNEAITGY---EHFSEGKCELFPQEAIQEWNEMMNERQVIGQIQAQLFAQGGQFPQ 635

Query: 63  SSYRACNCSNILQMVGNNNHIRCWSCQNHYCYLCKKVVKRSSQHYGPKGCKQHTAG 106
                 NC       GNNNH+ CW+CQ H+CYLCKKVVK+S+QHYGPKGCKQHT G
Sbjct: 636 RGQLCPNCRQFNGKAGNNNHLFCWACQAHFCYLCKKVVKKSAQHYGPKGCKQHTDG 688

The following BLAST results are available for this feature:

BLAST of Cp4.1LG15g02010.1 vs.


Match Name	E-value	Identity	Description

BLAST of Cp4.1LG15g02010.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023554779.1	1.86e-24	54.55	E3 ubiquitin-protein ligase RNF14 [Cucurbita pepo subsp. pepo]	[more]
KAG6571648.1	2.48e-24	90.00	E3 ubiquitin-protein ligase RNF14, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022963554.1	6.51e-24	88.00	E3 ubiquitin-protein ligase RNF14 [Cucurbita moschata]	[more]
KAG7011377.1	6.51e-24	88.00	E3 ubiquitin-protein ligase RNF14 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022967205.1	8.89e-24	86.00	E3 ubiquitin-protein ligase RNF14 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG15g02010.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HKI4	3.15e-24	88.00	E3 ubiquitin-protein ligase RNF14 OS=Cucurbita moschata OX=3662 GN=LOC111463847 ...	[more]
A0A6J1HTS2	4.30e-24	86.00	E3 ubiquitin-protein ligase RNF14 OS=Cucurbita maxima OX=3661 GN=LOC111466811 PE...	[more]
A0A314YBI4	2.50e-23	48.18	E3 ubiquitin-protein ligase RNF14 OS=Prunus yedoensis var. nudiflora OX=2094558 ...	[more]
A0A6J1DZW6	3.37e-22	83.33	E3 ubiquitin-protein ligase RNF14 OS=Momordica charantia OX=3673 GN=LOC111025870...	[more]
A0A1S3CQI9	6.31e-22	49.09	E3 ubiquitin-protein ligase RNF14 OS=Cucumis melo OX=3656 GN=LOC103503565 PE=3 S...	[more]

BLAST of Cp4.1LG15g02010.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32340.1	4.3e-18	41.38	NDR1/HIN1-like 8	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 55..102

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG15g02010	Cp4.1LG15g02010	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG15g02010.1:three_prime_utr:001	Cp4.1LG15g02010.1:three_prime_utr:001	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG15g02010.1:exon:003	Cp4.1LG15g02010.1:exon:003	exon
Cp4.1LG15g02010.1:exon:002	Cp4.1LG15g02010.1:exon:002	exon
Cp4.1LG15g02010.1:exon:001	Cp4.1LG15g02010.1:exon:001	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG15g02010.1:cds:001	Cp4.1LG15g02010.1:cds:001	CDS
Cp4.1LG15g02010.1:cds:002	Cp4.1LG15g02010.1:cds:002	CDS
Cp4.1LG15g02010.1:cds:003	Cp4.1LG15g02010.1:cds:003	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG15g02010.1	Cp4.1LG15g02010.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity